RLG00000015175

Aspartyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007392
Physical Location & Seq
Forward (+)
62683442 .. 62688817
5376 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015175

Sequence Viewer

Length: 1833 bp
ATGTCCCTCCTCCGCAGAACCTTCTTCCCTCTCCTCCCTCTCCGCTCTAAACCCCTCCCTCTTCTCTCCCACACCCTCCTCTTTCTTAAACCCTCTCTCTCTAAACCCCTCCCCATCCACATCTCCATTCTCTCCTCCCCAATTTCATCTTCAGCTTCAGCTTCAGCTTCTTCCACAACCCCACCTCCCACAGAAACCCTCACTCCCAAACCCCAATCTCCCCTCCTCTGGGTCCCCCGCACCGCCTTCTGCGGCCAGCTCTCCGCCGATGACGTCGGCAAACGCGTCACTCTCTGCGGCTGGGTCGCCCTCCACCGCGTCCACGGCGGCCTCACTTTCCTCAACCTCAGGGACCACACCGGCTCCGTCCAGGTCACCACTCTCCCCGACGCTTTTCCCGAGGCGCATTCCGCCGTAAACGAATTGAGGCTCGAGTACGTCGTCGCCGTTGAAGGCCTCGTCCGGTCTCGACCGGCCGACTCCGTCAACAAGAAAATGAAAACCGGCTCACTTGAGATTGCGGCGGAGAATGTTCAAGTGTTGAATGCAGTGAGGGCAAAGCTGCCATTTTTAGTTACTACTGTGGAGGATGCAAAGGATTCGGTGAAGGAGGAGATTCGGTTAAGGTACCGGTGTCTTGACTTACGCCGGCAGCAAATGCAATCCAACATAATGCTGCGGCATAAGGTGGTGAAACTGATGAGAAGGTATCTAGAGGATGTGCATGATTTTGTGGAGGTTGAGACTCCAATACTCTCAAGATCTACACCTGAAGGAGCCCGAGATTATTTAGTACCCTCGAGAGTTCAGCCAGGGACATTTTATGCTTTGCCTCAAAGTCCGCAACTGTTCAAGCAAATGTTGATGGTTTCTGGTTTTGATAAGTATTATCAGATAGCAAGATGTTTTCGAGATGAAGATTTACGAGCTGATAGGCAGCCTGAGTTTACACAGCTTGATATGGAGCTCGCTTTCACTCCTTTGGAGGACATGCTGAGGCTTAATGAAGATTTGATTAGAAAGGTTTTCTTAGAGATCAAAGGTGTACATCTCCCAAACCCTTTTCCTAGGCTTACGTATGCTGAAGCGATGAGTCGATATGGTTCGGACAGGCCAGATATTAGATTTGATCTTGAGCTGAAAGATGTATCTGATATTTTCTCAGATTCCACATTCAGGGTTTTTGCTGATACTTTGACAAGTAGAGGAATTATAAAAGTTTTATGTGTGCCTTCTGGAGCTAAAAGATATTCGAACACAGCTCTAAAGAAGGGAGATATTTATAATCAAGCAATCAAGTCTGGAGCCAAGGGTTTGCCTTTCCTGAAGGTCTTGGATGATGGTGGAATTGAGGGAATTCCTGCATTAACATCTAGTTTGGATCAAACAAAGATAGAGCAATTTCTGAGTCGTTGCTCTGCCAGACCAGGGGATCTGATCTTGTTTGCAGTGGGTCATCAGGCATCAGTTAATAAAACCCTAGATCGATTACGGGTCTTTGTTGCACATGAGCTTGATTTGATTGATGATTCCAGGCATTCAGTTCTGTGGGTGACTGATTTCCCAATGTTTGAATGGAATGATTTAGAGCAAAGGCTCGAGGCCTTGCACCACCCCTTCACTGCCCCGAACCCTGAAGACATAAATGATCTTTCTACAGCTCGAGGAATCGCCTATGGCTTGGATAGATTGGTCATGTTGTTGGCCGGTGCTAGTTCCATTAGGGATGTCATAGCTTTCCCGAAAACTACAACCGCACAATGTGCCCTTACCAGATCACCGTCTGAGGTGGATCCCCAGCAGCTAAAGGATTTGTCATTTCAGACTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
GAD tRNA-synt_2 tRNA_anti-codon

Protein Analysis

611

Amino Acids

68.46

Weight (kDa)

8.33

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 96 - 181 2.6e-13 OB-fold nucleic acid binding domain
tRNA-synt_2 PF00152 203 - 549 1.4e-82 tRNA synthetases class II (D, K and N)
GAD PF02938 395 - 494 8.9e-18 GAD domain
tRNA-synt_2 PF00152 556 - 583 4.6e-08 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1214, 1284
AatII GACGTC 1 cut(s) 276
Acc65I GGTACC 1 cut(s) 627
AccB1I GGYRCC 1 cut(s) 627
AccBSI CCGCTC 1 cut(s) 45
AccII CGCG 2 cut(s) 285, 318
AclWI GGATC 4 cut(s) 1389, 1440, 1787, 1800
AcoI YGGCCR 3 cut(s) 253, 474, 1704
AcsI RAATTY 1 cut(s) 1356
AcuI CTGAAG 7 cut(s) 135, 141, 147, 792, 1104, 1346, 1656
AcyI GRCGYC 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 1763
AfaI GTAC 4 cut(s) 437, 629, 795, 1047
AfiI CCNNNNNNNGG 4 cut(s) 228, 229, 1176, 1428
AflIII ACRYGT 1 cut(s) 283
AgeI ACCGGT 1 cut(s) 630
AgsI TTSAA 5 cut(s) 452, 536, 544, 853, 1574
AjnI CCWGG 4 cut(s) 369, 811, 1426, 1532
AjuI GAANNNNNNNTTGG 2 cut(s) 133, 165
Alw21I GWGCWC 1 cut(s) 969
Alw26I GTCTC 2 cut(s) 471, 737
AlwI GGATC 4 cut(s) 1389, 1440, 1787, 1800
Ama87I CYCGRG 6 cut(s) 398, 431, 780, 799, 1598, 1662
AoxI GGCC 7 cut(s) 253, 328, 454, 474, 1112, 1602, 1704
ApeKI GCWGC 5 cut(s) 562, 652, 676, 937, 1801
ApoI RAATTY 1 cut(s) 1356
AsiGI ACCGGT 1 cut(s) 630
Asp718I GGTACC 1 cut(s) 627
AspA2I CCTAGG 1 cut(s) 1067
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 2 cut(s) 232, 352
AsuHPI GGTGA 5 cut(s) 367, 616, 703, 1564, 1770
AsuII TTCGAA 1 cut(s) 1253
AvaI CYCGRG 6 cut(s) 398, 431, 780, 799, 1598, 1662
AvaII GGWCC 2 cut(s) 232, 352
AvrII CCTAGG 1 cut(s) 1067
AxyI CCTNAGG 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 1768
BaeI ACNNNNGTAYC 2 cut(s) 619, 652
BamHI GGATCC 1 cut(s) 1792
BanI GGYRCC 1 cut(s) 627
BanII GRGCYC 2 cut(s) 781, 969
BbsI GAAGAC 1 cut(s) 1644
Bbv12I GWGCWC 1 cut(s) 969
BbvCI CCTCAGC 1 cut(s) 995
BbvI GCAGC 5 cut(s) 549, 663, 664, 949, 1813
BccI CCATC 3 cut(s) 122, 859, 1334
BceAI ACGGC 3 cut(s) 340, 398, 431
BcgI CGANNNNNNTGC 2 cut(s) 582, 616
BciT130I CCWGG 4 cut(s) 371, 813, 1428, 1534
BcoDI GTCTC 2 cut(s) 471, 737
BfaI CTAG 5 cut(s) 713, 1068, 1374, 1481, 1713
BfmI CTRYAG 1 cut(s) 1656
BglII AGATCT 1 cut(s) 761
BlnI CCTAGG 1 cut(s) 1067
Bme1390I CCNGG 4 cut(s) 371, 813, 1428, 1534
Bme18I GGWCC 2 cut(s) 232, 352
BmeT110I CYCGRG 6 cut(s) 398, 431, 780, 799, 1598, 1662
BmgT120I GGNCC 2 cut(s) 232, 352
BmiI GGNNCC 8 cut(s) 233, 234, 353, 364, 629, 778, 1306, 1794
BmrFI CCNGG 4 cut(s) 371, 813, 1428, 1534
BmsI GCATC 2 cut(s) 580, 1472
BpiI GAAGAC 1 cut(s) 1644
BpmI CTGGAG 2 cut(s) 1257, 1323
Bpu10I CCTNAGC 1 cut(s) 995
Bpu14I TTCGAA 1 cut(s) 1253
BpuEI CTTGAG 3 cut(s) 533, 742, 1154
Bsa29I ATCGAT 1 cut(s) 1486
BsaAI YACGTR 1 cut(s) 1077
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 471
BsaJI CCNNGG 6 cut(s) 322, 399, 812, 1067, 1308, 1427
BsaWI WCCGGW 2 cut(s) 462, 630
BsaXI ACNNNNNCTCC 8 cut(s) 169, 187, 199, 217, 347, 377, 605, 635
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 229, 1176, 1428
Bse118I RCCGGY 6 cut(s) 359, 472, 503, 630, 648, 1706
Bse21I CCTNAGG 1 cut(s) 347
BseBI CCWGG 4 cut(s) 371, 813, 1428, 1534
BseCI ATCGAT 1 cut(s) 1486
BseDI CCNNGG 6 cut(s) 322, 399, 812, 1067, 1308, 1427
BseGI GGATG 5 cut(s) 114, 595, 724, 1342, 1732
BseLI CCNNNNNNNGG 4 cut(s) 228, 229, 1176, 1428
BseMII CTCAG 6 cut(s) 361, 933, 986, 1176, 1397, 1776
BseRI GAGGAG 5 cut(s) 23, 68, 124, 215, 626
BseSI GKGCMC 1 cut(s) 1768
BseX3I CGGCCG 1 cut(s) 474
BseXI GCAGC 5 cut(s) 549, 663, 664, 949, 1813
BseYI CCCAGC 2 cut(s) 300, 1797
Bsh1236I CGCG 2 cut(s) 285, 318
Bsh1285I CGRYCG 2 cut(s) 473, 477
BshFI GGCC 7 cut(s) 255, 330, 456, 476, 1114, 1604, 1706
BshNI GGYRCC 1 cut(s) 627
BshTI ACCGGT 1 cut(s) 630
BshVI ATCGAT 1 cut(s) 1486
BsiEI CGRYCG 2 cut(s) 473, 477
BsiHKAI GWGCWC 1 cut(s) 969
BsiHKCI CYCGRG 6 cut(s) 398, 431, 780, 799, 1598, 1662
BsiSI CCGG 7 cut(s) 360, 463, 473, 504, 631, 649, 1707
BslFI GGGAC 3 cut(s) 218, 365, 829
BslI CCNNNNNNNGG 4 cut(s) 228, 229, 1176, 1428
BsmAI GTCTC 2 cut(s) 471, 737
BsmFI GGGAC 3 cut(s) 218, 365, 829
BsmI GAATGC 3 cut(s) 406, 550, 1537
BsnI GGCC 7 cut(s) 255, 330, 456, 476, 1114, 1604, 1706
Bso31I GGTCTC 1 cut(s) 471
BsoBI CYCGRG 6 cut(s) 398, 431, 780, 799, 1598, 1662
Bsp119I TTCGAA 1 cut(s) 1253
Bsp1286I GDGCHC 3 cut(s) 781, 969, 1768
Bsp1407I TGTACA 1 cut(s) 1045
BspANI GGCC 7 cut(s) 255, 330, 456, 476, 1114, 1604, 1706
BspCNI CTCAG 6 cut(s) 360, 934, 987, 1175, 1398, 1777
BspDI ATCGAT 1 cut(s) 1486
BspFNI CGCG 2 cut(s) 285, 318
BspLI GGNNCC 8 cut(s) 233, 234, 353, 364, 629, 778, 1306, 1794
BspPI GGATC 4 cut(s) 1389, 1440, 1787, 1800
BspT104I TTCGAA 1 cut(s) 1253
BspT107I GGYRCC 1 cut(s) 627
BspTNI GGTCTC 1 cut(s) 471
BsrBI CCGCTC 1 cut(s) 45
BsrFI RCCGGY 6 cut(s) 359, 472, 503, 630, 648, 1706
BsrGI TGTACA 1 cut(s) 1045
BssAI RCCGGY 6 cut(s) 359, 472, 503, 630, 648, 1706
BssECI CCNNGG 6 cut(s) 322, 399, 812, 1067, 1308, 1427
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 2 cut(s) 1067, 1308
Bst2UI CCWGG 4 cut(s) 371, 813, 1428, 1534
Bst4CI ACNGT 3 cut(s) 583, 849, 1782
Bst6I CTCTTC 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 273
BstAPI GCANNNNNTGC 2 cut(s) 658, 1763
BstAUI TGTACA 1 cut(s) 1045
BstBAI YACGTR 1 cut(s) 1077
BstBI TTCGAA 1 cut(s) 1253
BstC8I GCNNGC 3 cut(s) 257, 650, 969
BstDEI CTNAG 7 cut(s) 347, 942, 995, 1030, 1162, 1406, 1785
BstDSI CCRYGG 1 cut(s) 322
BstEII GGTNACC 1 cut(s) 373
BstF5I GGATG 5 cut(s) 114, 595, 724, 1342, 1732
BstFNI CGCG 2 cut(s) 285, 318
BstHHI GCGC 1 cut(s) 406
BstMAI GTCTC 2 cut(s) 471, 737
BstMCI CGRYCG 2 cut(s) 473, 477
BstMWI GCNNNNNNNGC 5 cut(s) 324, 410, 554, 658, 1763
BstNI CCWGG 4 cut(s) 371, 813, 1428, 1534
BstNSI RCATGY 1 cut(s) 994
BstPI GGTNACC 1 cut(s) 373
BstSCI CCNGG 4 cut(s) 369, 811, 1426, 1532
BstSFI CTRYAG 1 cut(s) 1656
BstSLI GKGCMC 1 cut(s) 1768
BstSNI TACGTA 1 cut(s) 1077
BstUI CGCG 2 cut(s) 285, 318
BstV1I GCAGC 5 cut(s) 549, 663, 664, 949, 1813
BstV2I GAAGAC 1 cut(s) 1644
BstX2I RGATCY 3 cut(s) 761, 1432, 1792
BstYI RGATCY 3 cut(s) 761, 1432, 1792
BstZI CGGCCG 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 1486
Bsu36I CCTNAGG 1 cut(s) 347
BsuRI GGCC 7 cut(s) 255, 330, 456, 476, 1114, 1604, 1706
BsuTUI ATCGAT 1 cut(s) 1486
BtgI CCRYGG 1 cut(s) 322
BtgZI GCGATG 1 cut(s) 1103
BtsCI GGATG 5 cut(s) 114, 595, 724, 1342, 1732
BtsI GCAGTG 3 cut(s) 555, 1455, 1620
BtsIMutI CAGTG 3 cut(s) 555, 1455, 1620
Cac8I GCNNGC 3 cut(s) 257, 650, 969
CfoI GCGC 1 cut(s) 406
Cfr10I RCCGGY 6 cut(s) 359, 472, 503, 630, 648, 1706
Cfr13I GGNCC 2 cut(s) 232, 352
ClaI ATCGAT 1 cut(s) 1486
CseI GACGC 3 cut(s) 274, 307, 398
Csp6I GTAC 4 cut(s) 436, 628, 794, 1046
CspAI ACCGGT 1 cut(s) 630
CviAII CATG 4 cut(s) 725, 991, 1508, 1696
CviQI GTAC 4 cut(s) 436, 628, 794, 1046
DdeI CTNAG 7 cut(s) 347, 942, 995, 1030, 1162, 1406, 1785
DraIII CACNNNGTG 1 cut(s) 1763
EaeI YGGCCR 3 cut(s) 253, 474, 1704
EagI CGGCCG 1 cut(s) 474
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
EciI GGCGGA 3 cut(s) 253, 400, 539
Ecl136II GAGCTC 1 cut(s) 967
EclXI CGGCCG 1 cut(s) 474
Eco105I TACGTA 1 cut(s) 1077
Eco130I CCWWGG 2 cut(s) 1067, 1308
Eco147I AGGCCT 2 cut(s) 456, 1604
Eco24I GRGCYC 2 cut(s) 781, 969
Eco31I GGTCTC 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 232, 352
Eco52I CGGCCG 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 967
Eco57I CTGAAG 7 cut(s) 135, 141, 147, 792, 1104, 1346, 1656
Eco81I CCTNAGG 1 cut(s) 347
Eco88I CYCGRG 6 cut(s) 398, 431, 780, 799, 1598, 1662
Eco91I GGTNACC 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 967
EcoO109I RGGNCCY 1 cut(s) 232
EcoO65I GGTNACC 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 1356
EcoRII CCWGG 4 cut(s) 369, 811, 1426, 1532
EcoT14I CCWWGG 2 cut(s) 1067, 1308
EcoT38I GRGCYC 2 cut(s) 781, 969
ErhI CCWWGG 2 cut(s) 1067, 1308
FaeI CATG 4 cut(s) 728, 994, 1511, 1699
FalI AAGNNNNNCTT 2 cut(s) 1013, 1045
FaqI GGGAC 3 cut(s) 218, 365, 829
FatI CATG 4 cut(s) 724, 990, 1507, 1695
FauI CCCGC 1 cut(s) 245
FokI GGATG 5 cut(s) 101, 602, 731, 1349, 1739
FriOI GRGCYC 2 cut(s) 781, 969
FspBI CTAG 5 cut(s) 713, 1068, 1374, 1481, 1713
GlaI GCGC 1 cut(s) 405
GsaI CCCAGC 2 cut(s) 304, 1801
GsuI CTGGAG 2 cut(s) 1257, 1323
HaeIII GGCC 7 cut(s) 255, 330, 456, 476, 1114, 1604, 1706
HapII CCGG 7 cut(s) 360, 463, 473, 504, 631, 649, 1707
HgaI GACGC 3 cut(s) 274, 307, 398
HhaI GCGC 1 cut(s) 406
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 4 cut(s) 728, 994, 1511, 1699
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HpaII CCGG 7 cut(s) 360, 463, 473, 504, 631, 649, 1707
HphI GGTGA 5 cut(s) 367, 616, 703, 1564, 1770
Hpy166II GTNNAC 5 cut(s) 322, 418, 487, 948, 1046
Hpy188I TCNGA 8 cut(s) 894, 1108, 1153, 1165, 1407, 1437, 1786, 1824
Hpy8I GTNNAC 5 cut(s) 322, 418, 487, 948, 1046
Hpy99I CGWCG 4 cut(s) 278, 392, 443, 446
HpyCH4III ACNGT 3 cut(s) 583, 849, 1782
HpyCH4IV ACGT 3 cut(s) 273, 438, 1076
HpyCH4V TGCA 8 cut(s) 548, 593, 661, 724, 1364, 1448, 1505, 1609
HpyF10VI GCNNNNNNNGC 5 cut(s) 324, 410, 554, 658, 1763
HpyF3I CTNAG 7 cut(s) 347, 942, 995, 1030, 1162, 1406, 1785
HpySE526I ACGT 3 cut(s) 273, 438, 1076
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 4 cut(s) 728, 994, 1511, 1699
HspAI GCGC 1 cut(s) 404
KflI GGGWCCC 1 cut(s) 232
KpnI GGTACC 1 cut(s) 631
KroI GCCGGC 1 cut(s) 648
KroNI GCCGGC 1 cut(s) 650
LmnI GCTCC 5 cut(s) 368, 776, 964, 1238, 1304
Lsp1109I GCAGC 5 cut(s) 549, 663, 664, 949, 1813
LweI GCATC 2 cut(s) 580, 1472
MaeI CTAG 5 cut(s) 713, 1068, 1374, 1481, 1713
MaeII ACGT 3 cut(s) 273, 438, 1076
MaeIII GTNAC 4 cut(s) 286, 373, 574, 1552
MbiI CCGCTC 1 cut(s) 45
MboII GAAGA 7 cut(s) 16, 53, 141, 162, 929, 1019, 1649
MflI RGATCY 3 cut(s) 761, 1432, 1792
MhlI GDGCHC 3 cut(s) 781, 969, 1768
MluCI AATT 6 cut(s) 141, 422, 1209, 1347, 1356, 1400
MluI ACGCGT 1 cut(s) 283
MlyI GAGTC 5 cut(s) 473, 739, 1102, 1417, 1819
MmeI TCCRAC 1 cut(s) 690
MroNI GCCGGC 1 cut(s) 648
MseI TTAA 5 cut(s) 87, 623, 1002, 1367, 1470
MspI CCGG 7 cut(s) 360, 463, 473, 504, 631, 649, 1707
MspR9I CCNGG 4 cut(s) 371, 813, 1428, 1534
Mva1269I GAATGC 3 cut(s) 406, 550, 1537
MvaI CCWGG 4 cut(s) 371, 813, 1428, 1534
MvnI CGCG 2 cut(s) 285, 318
MwoI GCNNNNNNNGC 5 cut(s) 324, 410, 554, 658, 1763
NaeI GCCGGC 1 cut(s) 650
NgoMIV GCCGGC 1 cut(s) 648
NlaIII CATG 4 cut(s) 728, 994, 1511, 1699
NlaIV GGNNCC 8 cut(s) 233, 234, 353, 364, 629, 778, 1306, 1794
NmuCI GTSAC 3 cut(s) 286, 373, 1552
NspI RCATGY 1 cut(s) 994
NspV TTCGAA 1 cut(s) 1253
PaeR7I CTCGAG 4 cut(s) 431, 799, 1598, 1662
PceI AGGCCT 2 cut(s) 456, 1604
PcsI WCGNNNNNNNCGW 4 cut(s) 282, 396, 438, 444
PctI GAATGC 3 cut(s) 406, 550, 1537
PdiI GCCGGC 1 cut(s) 650
PfeI GAWTC 5 cut(s) 599, 616, 1166, 1529, 1668
PflFI GACNNNGTC 1 cut(s) 482
PinAI ACCGGT 1 cut(s) 630
PleI GAGTC 5 cut(s) 473, 739, 1101, 1416, 1819
PpsI GAGTC 5 cut(s) 473, 739, 1101, 1416, 1819
Ppu21I YACGTR 1 cut(s) 1077
PpuMI RGGWCCY 1 cut(s) 232
PsiI TTATAA 2 cut(s) 1214, 1284
Psp124BI GAGCTC 1 cut(s) 969
Psp5II RGGWCCY 1 cut(s) 232
Psp6I CCWGG 4 cut(s) 369, 811, 1426, 1532
PspEI GGTNACC 1 cut(s) 373
PspFI CCCAGC 2 cut(s) 300, 1797
PspGI CCWGG 4 cut(s) 369, 811, 1426, 1532
PspN4I GGNNCC 8 cut(s) 233, 234, 353, 364, 629, 778, 1306, 1794
PspPI GGNCC 2 cut(s) 232, 352
PspPPI RGGWCCY 1 cut(s) 232
PspXI VCTCGAGB 3 cut(s) 431, 1598, 1662
PsuI RGATCY 3 cut(s) 761, 1432, 1792
PsyI GACNNNGTC 1 cut(s) 482
RsaI GTAC 4 cut(s) 437, 629, 795, 1047
RsaNI GTAC 4 cut(s) 436, 628, 794, 1046
SacI GAGCTC 1 cut(s) 969
SaqAI TTAA 5 cut(s) 87, 623, 1002, 1367, 1470
Sau96I GGNCC 2 cut(s) 232, 352
SchI GAGTC 5 cut(s) 473, 739, 1102, 1417, 1819
ScrFI CCNGG 4 cut(s) 371, 813, 1428, 1534
SduI GDGCHC 3 cut(s) 781, 969, 1768
SfaNI GCATC 2 cut(s) 580, 1472
SfcI CTRYAG 1 cut(s) 1656
Sfr274I CTCGAG 4 cut(s) 431, 799, 1598, 1662
SfuI TTCGAA 1 cut(s) 1253
SinI GGWCC 2 cut(s) 232, 352
SlaI CTCGAG 4 cut(s) 431, 799, 1598, 1662
SmlI CTYRAG 7 cut(s) 431, 512, 757, 799, 1133, 1598, 1662
SmoI CTYRAG 7 cut(s) 431, 512, 757, 799, 1133, 1598, 1662
SnaBI TACGTA 1 cut(s) 1077
Sse9I AATT 6 cut(s) 141, 422, 1209, 1347, 1356, 1400
SseBI AGGCCT 2 cut(s) 456, 1604
SspMI CTAG 5 cut(s) 713, 1068, 1374, 1481, 1713
SstI GAGCTC 1 cut(s) 969
StuI AGGCCT 2 cut(s) 456, 1604
StyD4I CCNGG 4 cut(s) 369, 811, 1426, 1532
StyI CCWWGG 2 cut(s) 1067, 1308
TaaI ACNGT 3 cut(s) 583, 849, 1782
TaiI ACGT 3 cut(s) 276, 441, 1079
TaqI TCGA 9 cut(s) 432, 469, 800, 910, 1096, 1253, 1486, 1599, 1663
TasI AATT 6 cut(s) 141, 422, 1209, 1347, 1356, 1400
TatI WGTACW 1 cut(s) 1045
TauI GCSGC 5 cut(s) 255, 300, 330, 524, 682
TfiI GAWTC 5 cut(s) 599, 616, 1166, 1529, 1668
Tru1I TTAA 5 cut(s) 87, 623, 1002, 1367, 1470
Tru9I TTAA 5 cut(s) 87, 623, 1002, 1367, 1470
TscAI CASTG 3 cut(s) 555, 1455, 1627
TseFI GTSAC 3 cut(s) 286, 373, 1552
TseI GCWGC 5 cut(s) 562, 652, 676, 937, 1801
Tsp45I GTSAC 3 cut(s) 286, 373, 1552
TspDTI ATGAA 4 cut(s) 135, 512, 930, 1020
TspGWI ACGGA 2 cut(s) 355, 472
TspRI CASTG 3 cut(s) 555, 1455, 1627
Tth111I GACNNNGTC 1 cut(s) 482
VpaK11BI GGWCC 2 cut(s) 232, 352
XapI RAATTY 1 cut(s) 1356
XbaI TCTAGA 1 cut(s) 712
XceI RCATGY 1 cut(s) 994
XcmI CCANNNNNNNNNTGG 1 cut(s) 1572
XhoI CTCGAG 4 cut(s) 431, 799, 1598, 1662
XmaJI CCTAGG 1 cut(s) 1067
XspI CTAG 5 cut(s) 713, 1068, 1374, 1481, 1713
ZraI GACGTC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.