Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007392

Physical Location & Seq

Genomic Coordinates Forward (+)

12905988 .. 12910685

Gene Structure 4698 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g09720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGTCCCTCCTGCGCAGAACCTTCCCTCTCCTCCCCCTCCGCCCCAAACACCTCCCTCTCCTATCCCACTCCTTCCTTTTCCTCCAACCATCCTTCCCTAAACCCCTTCCCAAACACACCTCCATCTTCTCTTCCTCCGCTTCCGCTTCCGCTTCCACTTCCACTTCCACACCCATTCCCAATCCCACCCCTAATCCCCCAATTTCTCCAACCCAACTCCAATGGATCACCAGAACCGCCTCCTGCGGCGACCTATCCACGGCCGACGTCGGCAAACGCGTCACTCTCTGCGGGTGGGTCGCCCTCCACCGTGTCCACGGCGGCCTCACCTTTCTCAACCTCCGTGACCACACCGGTTCCGTCCAGGTCACCACTCTCCCCGACACTTTCCCCGACGCTCATTCCGCCGTTAACGACCTGAGGCTCGAGTACGTCGTAGCCATTGAAGGACTCGTACGGTCTAGACCGGCCGACTCCGTCAACAAGAAGATGAAAACCGGGTTAATTGAGATTGCGGCGGAGAGCGTTCAAGTGTTGAATGCCGTTAGAACAAAGCTGCCATTTTTGGTCACTACTGCTGATGATGCTAAGGATTCTGCAAAGGAGGAGATACGGTTAAGGTACCGGTGCCTAGATTTACGCCGGCAGCAAATGCATTCGAACATAATGTTGCGGCATAAAGTTGTGAAATTGATTCGAAGACATCTAGAGGATGCACATGGTTTTGTGGAGATTGAGACTCCAATACTCTCTAGGTCTACACCCGAAGGTGCTCGAGATTATTTAGTGCCCTCGAGAGTTCAGCCGGGAACATTTTATGCTTTGCCTCAAAGTCCGCAACTGTTCAAGCAAATGTTGATGGTTTCTGGTTTTGATAAGTATTATCAAATAGCAAGGTGCTTTCGAGATGAAGATCTAAGAGCTGATAGACAACCTGAGTTCACACAGCTTGATATGGAGCTTGCTTTCACTCCTTTGGAGGAGATGCTGAAGCTTAATGAAGATTTGATTAGAAAGGTTTTTCTAGAGATCAAAGGTGTAAATCTCCCAAACCCTTTTCCTAGGCTTACGTATGCTGAAGCAATGAGTAGATATGGTTCAGACAGGCCAGATATTAGATTTGATCTAGAGTTAAAAGATGTATCTGATATTTTCTCAGATTCCCCGTTCCGGGTTTTTGCTGATACTTTGAAAAGTGGGGGAGTTATCAAAGTTTTATGCATACCTTCAGGTGGTAAAAGATATTCAAACACGGCGCTAAAGAAGGGTGATGTTTACAATGAAGCAATCAAATCTGGAGCAAAGGGTTTGCCTTTCCTGAAGGTCTTGAATGATGGGGGAATGGAGGGAATTCCTGCATTGGTATCTAGTTTGGACCCAACAAAGACACAGCAGTTTCTGAGTCGTTGCTCTGCCAGACCAGGTGATCTTATCTTGTTTGCAGTGGGTCATCATACATCAGTTAATAAAACTCTAGATCGACTACGGCTCTTTATGGCACATGAACTTGGTTTGGTTGATGATGCCTTGCACCACCCTTTCACTGCCCCGAATCCTGAAGACATACAAGATCTGTCTTCTGCTCGTGCTTTAGCTTATGACATGGTTTACAATGGAGTTGAGATTGGTGGGGGAAGTTTGAGAATTTATAAACGCGATGTGCAGCAAAAGGTTTTGGAGATTGTTGGCATCTCCCCTGAAGAGGCTGAAACGAAGTTTGGCTATCTTCTCGAGGCATTAGACATGGGTGCCCCTCCACATGGGGGAATTGCATATGGTTTGGACAGATTGGTTATGTTGTTAGCAGGGGCTAATTCCATTAGGGACGTCATAGCTTTCCCAAAGACAACCACCGCACAGTGTGCTCTCACTCGAACACCATCTCCGGTGGATCCCCAGCAGCTAAAAGACCTATCATACCAGACTCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

GAD tRNA-synt_2 tRNA_anti-codon

Protein Analysis

644

Amino Acids

71.38

Weight (kDa)

8.62

Isoelectric Point (pI)

48.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	94 - 179	2.1e-12	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	201 - 616	2.8e-116	tRNA synthetases class II (D, K and N)
GAD	PF02938	393 - 492	3.6e-17	GAD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33760 AT4G33760
fragaria_vesca	FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040
malus_domestica	MD10G1112200.v1.1
prunus_persica	Prupe.8G151800_v2.0.a1
pyrus_communis	pycom10g09720
rosa_chinensis	RchiOBHm_Chr6g0247831
rosa_laevigata	RLG00000015175
rosa_multiflora	Rmu_sc0001048.1_g000005 Rmu_sc0035971.1_g000001
rosa_roxburghii	Rroxscaffold_180G00433200 Rroxscaffold_7G00213610
rosa_rugosa	Rorug05G0529400
rosa_samantha	Rh6AG044100 Rh6BG038800 Rh6CG037600 Rh6DG034600
rosa_wichuraiana	Rw6G003820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1650
AatII	GACGTC	2 cut(s)	270, 1830
Acc16I	TGCGCA	1 cut(s)	14
Acc65I	GGTACC	1 cut(s)	621
AccB1I	GGYRCC	3 cut(s)	621, 627, 1748
AccI	GTMKAC	1 cut(s)	758
AccII	CGCG	2 cut(s)	279, 1656
AclWI	GGATC	3 cut(s)	233, 1886, 1899
AcoI	YGGCCR	2 cut(s)	261, 468
AcsI	RAATTY	2 cut(s)	1350, 1644
AcuI	CTGAAG	6 cut(s)	1010, 1098, 1212, 1340, 1578, 1719
AcyI	GRCGYC	2 cut(s)	267, 1827
AdeI	CACNNNGTG	1 cut(s)	1862
AfaI	GTAC	3 cut(s)	431, 456, 623
AfiI	CCNNNNNNNGG	7 cut(s)	259, 1170, 1171, 1232, 1702, 1760, 1763
AflIII	ACRYGT	1 cut(s)	277
AgeI	ACCGGT	2 cut(s)	353, 624
AgsI	TTSAA	7 cut(s)	446, 530, 538, 847, 1192, 1248, 1330
AjnI	CCWGG	2 cut(s)	363, 1420
AjuI	GAANNNNNNNTTGG	2 cut(s)	1701, 1733
AloI	GAACNNNNNNTCC	2 cut(s)	1867, 1899
AluBI	AGCT	8 cut(s)	556, 923, 949, 961, 994, 1595, 1835, 1903
AluI	AGCT	8 cut(s)	556, 923, 949, 961, 994, 1595, 1835, 1903
Alw21I	GWGCWC	2 cut(s)	775, 1867
Alw26I	GTCTC	1 cut(s)	731
AlwI	GGATC	3 cut(s)	233, 1886, 1899
AlwNI	CAGNNNCTG	1 cut(s)	1399
Ama87I	CYCGRG	4 cut(s)	425, 774, 793, 1730
AoxI	GGCC	4 cut(s)	261, 322, 468, 1106
ApeKI	GCWGC	4 cut(s)	556, 646, 1663, 1900
ApoI	RAATTY	2 cut(s)	1350, 1644
AsiGI	ACCGGT	2 cut(s)	353, 624
Asp718I	GGTACC	1 cut(s)	621
AspA2I	CCTAGG	1 cut(s)	1061
AspLEI	GCGC	2 cut(s)	15, 1258
AspS9I	GGNCC	1 cut(s)	1375
AsuC2I	CCSGG	3 cut(s)	499, 807, 1172
AsuHPI	GGTGA	5 cut(s)	220, 319, 361, 1280, 1436
AsuII	TTCGAA	2 cut(s)	659, 697
AvaI	CYCGRG	4 cut(s)	425, 774, 793, 1730
AvaII	GGWCC	1 cut(s)	1375
AvrII	CCTAGG	1 cut(s)	1061
AxyI	CCTNAGG	1 cut(s)	419
BaeGI	GKGCMC	2 cut(s)	792, 1753
BamHI	GGATCC	1 cut(s)	1891
BanI	GGYRCC	3 cut(s)	621, 627, 1748
BarI	GAAGNNNNNNTAC	2 cut(s)	438, 470
BauI	CACGAG	1 cut(s)	1584
BbsI	GAAGAC	3 cut(s)	706, 1566, 1569
Bbv12I	GWGCWC	2 cut(s)	775, 1867
BbvI	GCAGC	4 cut(s)	543, 658, 1675, 1912
BccI	CCATC	5 cut(s)	97, 131, 853, 1328, 1888
BceAI	ACGGC	6 cut(s)	276, 334, 392, 527, 1269, 1502
BciT130I	CCWGG	2 cut(s)	365, 1422
BcnI	CCSGG	3 cut(s)	499, 807, 1172
BcoDI	GTCTC	1 cut(s)	731
BfaI	CTAG	9 cut(s)	462, 632, 707, 753, 1025, 1062, 1127, 1368, 1475
BfoI	RGCGCY	1 cut(s)	1259
BglII	AGATCT	2 cut(s)	913, 1570
BisI	GCNGC	8 cut(s)	247, 322, 516, 557, 647, 674, 1664, 1901
BlnI	CCTAGG	1 cut(s)	1061
BlsI	GCNGC	8 cut(s)	248, 323, 517, 558, 648, 675, 1665, 1902
Bme1390I	CCNGG	5 cut(s)	365, 499, 807, 1172, 1422
Bme18I	GGWCC	1 cut(s)	1375
BmeT110I	CYCGRG	4 cut(s)	425, 774, 793, 1730
BmgT120I	GGNCC	1 cut(s)	1375
BmiI	GGNNCC	6 cut(s)	358, 623, 629, 1377, 1750, 1893
BmrFI	CCNGG	5 cut(s)	365, 499, 807, 1172, 1422
BmsI	GCATC	5 cut(s)	574, 703, 975, 1513, 1698
BpiI	GAAGAC	3 cut(s)	706, 1566, 1569
BpmI	CTGGAG	1 cut(s)	1317
Bpu10I	CCTNAGC	1 cut(s)	588
Bpu14I	TTCGAA	2 cut(s)	659, 697
BpuMI	CCSGG	3 cut(s)	499, 807, 1172
BsaAI	YACGTR	1 cut(s)	1071
BsaBI	GATNNNNATC	1 cut(s)	912
BsaHI	GRCGYC	2 cut(s)	267, 1827
BsaJI	CCNNGG	3 cut(s)	258, 316, 1061
BsaWI	WCCGGW	3 cut(s)	353, 624, 1885
BsaXI	ACNNNNNCTCC	4 cut(s)	599, 629, 1867, 1897
Bsc4I	CCNNNNNNNGG	7 cut(s)	259, 1170, 1171, 1232, 1702, 1760, 1763
Bse118I	RCCGGY	4 cut(s)	353, 466, 624, 642
Bse21I	CCTNAGG	1 cut(s)	419
Bse3DI	GCAATG	1 cut(s)	1089
Bse8I	GATNNNNATC	1 cut(s)	912
BseBI	CCWGG	2 cut(s)	365, 1422
BseDI	CCNNGG	3 cut(s)	258, 316, 1061
BseGI	GGATG	2 cut(s)	89, 718
BseJI	GATNNNNATC	1 cut(s)	912
BseLI	CCNNNNNNNGG	7 cut(s)	259, 1170, 1171, 1232, 1702, 1760, 1763
BseMI	GCAATG	1 cut(s)	1089
BseMII	CTCAG	4 cut(s)	410, 927, 1170, 1391
BseRI	GAGGAG	3 cut(s)	20, 620, 995
BseSI	GKGCMC	2 cut(s)	792, 1753
BseX3I	CGGCCG	2 cut(s)	261, 468
BseXI	GCAGC	4 cut(s)	543, 658, 1675, 1912
BseYI	CCCAGC	1 cut(s)	1896
BsgI	GTGCAG	1 cut(s)	1682
Bsh1236I	CGCG	2 cut(s)	279, 1656
Bsh1285I	CGRYCG	2 cut(s)	264, 471
BshFI	GGCC	4 cut(s)	263, 324, 470, 1108
BshNI	GGYRCC	3 cut(s)	621, 627, 1748
BshTI	ACCGGT	2 cut(s)	353, 624
BsiEI	CGRYCG	2 cut(s)	264, 471
BsiHKAI	GWGCWC	2 cut(s)	775, 1867
BsiHKCI	CYCGRG	4 cut(s)	425, 774, 793, 1730
BsiSI	CCGG	8 cut(s)	354, 467, 498, 625, 643, 806, 1171, 1886
BsiWI	CGTACG	1 cut(s)	454
BslFI	GGGAC	1 cut(s)	1838
BslI	CCNNNNNNNGG	7 cut(s)	259, 1170, 1171, 1232, 1702, 1760, 1763
BsmAI	GTCTC	1 cut(s)	731
BsmFI	GGGAC	1 cut(s)	1838
BsmI	GAATGC	2 cut(s)	544, 655
BsnI	GGCC	4 cut(s)	263, 324, 470, 1108
BsoBI	CYCGRG	4 cut(s)	425, 774, 793, 1730
Bsp119I	TTCGAA	2 cut(s)	659, 697
Bsp1286I	GDGCHC	4 cut(s)	775, 792, 1753, 1867
Bsp143I	GATC	8 cut(s)	225, 913, 1029, 1123, 1426, 1477, 1570, 1891
BspANI	GGCC	4 cut(s)	263, 324, 470, 1108
BspCNI	CTCAG	4 cut(s)	411, 928, 1169, 1392
BspFNI	CGCG	2 cut(s)	279, 1656
BspLI	GGNNCC	6 cut(s)	358, 623, 629, 1377, 1750, 1893
BspPI	GGATC	3 cut(s)	233, 1886, 1899
BspT104I	TTCGAA	2 cut(s)	659, 697
BspT107I	GGYRCC	3 cut(s)	621, 627, 1748
BsrDI	GCAATG	1 cut(s)	1089
BsrFI	RCCGGY	4 cut(s)	353, 466, 624, 642
BssAI	RCCGGY	4 cut(s)	353, 466, 624, 642
BssECI	CCNNGG	3 cut(s)	258, 316, 1061
BssMI	GATC	8 cut(s)	225, 913, 1029, 1123, 1426, 1477, 1570, 1891
BssNI	GRCGYC	2 cut(s)	267, 1827
BssSI	CACGAG	1 cut(s)	1584
BssT1I	CCWWGG	1 cut(s)	1061
Bst2BI	CACGAG	1 cut(s)	1584
Bst2UI	CCWGG	2 cut(s)	365, 1422
Bst4CI	ACNGT	5 cut(s)	311, 459, 615, 843, 1860
Bst6I	CTCTTC	2 cut(s)	136, 1695
BstACI	GRCGYC	2 cut(s)	267, 1827
BstAPI	GCANNNNNTGC	2 cut(s)	652, 1862
BstBAI	YACGTR	1 cut(s)	1071
BstBI	TTCGAA	2 cut(s)	659, 697
BstC8I	GCNNGC	2 cut(s)	644, 963
BstDEI	CTNAG	6 cut(s)	419, 588, 917, 936, 1156, 1400
BstDSI	CCRYGG	2 cut(s)	258, 316
BstEII	GGTNACC	1 cut(s)	367
BstF5I	GGATG	2 cut(s)	89, 718
BstFNI	CGCG	2 cut(s)	279, 1656
BstH2I	RGCGCY	1 cut(s)	1259
BstHHI	GCGC	2 cut(s)	15, 1258
BstKTI	GATC	8 cut(s)	228, 916, 1032, 1126, 1429, 1480, 1573, 1894
BstMAI	GTCTC	1 cut(s)	731
BstMBI	GATC	8 cut(s)	225, 913, 1029, 1123, 1426, 1477, 1570, 1891
BstMCI	CGRYCG	2 cut(s)	264, 471
BstMWI	GCNNNNNNNGC	4 cut(s)	404, 584, 652, 1862
BstNI	CCWGG	2 cut(s)	365, 1422
BstPI	GGTNACC	1 cut(s)	367
BstSCI	CCNGG	5 cut(s)	363, 497, 805, 1170, 1420
BstSLI	GKGCMC	2 cut(s)	792, 1753
BstSNI	TACGTA	1 cut(s)	1071
BstUI	CGCG	2 cut(s)	279, 1656
BstV1I	GCAGC	4 cut(s)	543, 658, 1675, 1912
BstV2I	GAAGAC	3 cut(s)	706, 1566, 1569
BstX2I	RGATCY	3 cut(s)	913, 1570, 1891
BstYI	RGATCY	3 cut(s)	913, 1570, 1891
BstZI	CGGCCG	2 cut(s)	261, 468
Bsu36I	CCTNAGG	1 cut(s)	419
BsuRI	GGCC	4 cut(s)	263, 324, 470, 1108
BtgI	CCRYGG	2 cut(s)	258, 316
BtgZI	GCGATG	1 cut(s)	1671
BtsCI	GGATG	2 cut(s)	89, 718
BtsI	GCAGTG	2 cut(s)	1449, 1542
BtsIMutI	CAGTG	3 cut(s)	1449, 1542, 1865
Cac8I	GCNNGC	2 cut(s)	644, 963
CaiI	CAGNNNCTG	1 cut(s)	1399
CfoI	GCGC	2 cut(s)	15, 1258
Cfr10I	RCCGGY	4 cut(s)	353, 466, 624, 642
Cfr13I	GGNCC	1 cut(s)	1375
CseI	GACGC	2 cut(s)	268, 404
CsiI	ACCWGGT	1 cut(s)	1420
Csp6I	GTAC	3 cut(s)	430, 455, 622
CspAI	ACCGGT	2 cut(s)	353, 624
CviAII	CATG	5 cut(s)	719, 1502, 1603, 1744, 1760
CviQI	GTAC	3 cut(s)	430, 455, 622
DdeI	CTNAG	6 cut(s)	419, 588, 917, 936, 1156, 1400
DpnI	GATC	8 cut(s)	227, 915, 1031, 1125, 1428, 1479, 1572, 1893
DpnII	GATC	8 cut(s)	225, 913, 1029, 1123, 1426, 1477, 1570, 1891
DraIII	CACNNNGTG	1 cut(s)	1862
EaeI	YGGCCR	2 cut(s)	261, 468
EagI	CGGCCG	2 cut(s)	261, 468
Eam1104I	CTCTTC	2 cut(s)	136, 1695
EarI	CTCTTC	2 cut(s)	136, 1695
EciI	GGCGGA	3 cut(s)	29, 394, 533
EclXI	CGGCCG	2 cut(s)	261, 468
Eco105I	TACGTA	1 cut(s)	1071
Eco130I	CCWWGG	1 cut(s)	1061
Eco47I	GGWCC	1 cut(s)	1375
Eco52I	CGGCCG	2 cut(s)	261, 468
Eco57I	CTGAAG	6 cut(s)	1010, 1098, 1212, 1340, 1578, 1719
Eco81I	CCTNAGG	1 cut(s)	419
Eco88I	CYCGRG	4 cut(s)	425, 774, 793, 1730
Eco91I	GGTNACC	1 cut(s)	367
EcoO65I	GGTNACC	1 cut(s)	367
EcoRI	GAATTC	1 cut(s)	1350
EcoRII	CCWGG	2 cut(s)	363, 1420
EcoT14I	CCWWGG	1 cut(s)	1061
EcoT22I	ATGCAT	2 cut(s)	657, 1223
ErhI	CCWWGG	1 cut(s)	1061
FaeI	CATG	5 cut(s)	722, 1505, 1606, 1747, 1763
FaqI	GGGAC	1 cut(s)	1838
FatI	CATG	5 cut(s)	718, 1501, 1602, 1743, 1759
FauI	CCCGC	1 cut(s)	284
FauNDI	CATATG	1 cut(s)	1774
FblI	GTMKAC	1 cut(s)	758
Fnu4HI	GCNGC	8 cut(s)	247, 322, 516, 557, 647, 674, 1664, 1901
FokI	GGATG	2 cut(s)	76, 725
Fsp4HI	GCNGC	8 cut(s)	247, 322, 516, 557, 647, 674, 1664, 1901
FspBI	CTAG	9 cut(s)	462, 632, 707, 753, 1025, 1062, 1127, 1368, 1475
FspI	TGCGCA	1 cut(s)	14
GlaI	GCGC	2 cut(s)	14, 1257
GluI	GCNGC	8 cut(s)	247, 322, 516, 557, 647, 674, 1664, 1901
GsaI	CCCAGC	1 cut(s)	1900
GsuI	CTGGAG	1 cut(s)	1317
HaeII	RGCGCY	1 cut(s)	1259
HaeIII	GGCC	4 cut(s)	263, 324, 470, 1108
HapII	CCGG	8 cut(s)	354, 467, 498, 625, 643, 806, 1171, 1886
HgaI	GACGC	2 cut(s)	268, 404
HhaI	GCGC	2 cut(s)	15, 1258
Hin1I	GRCGYC	2 cut(s)	267, 1827
Hin1II	CATG	5 cut(s)	722, 1505, 1606, 1747, 1763
Hin6I	GCGC	2 cut(s)	13, 1256
HinP1I	GCGC	2 cut(s)	13, 1256
HincII	GTYRAC	2 cut(s)	412, 481
HindII	GTYRAC	2 cut(s)	412, 481
HindIII	AAGCTT	1 cut(s)	992
HinfI	GANTC	9 cut(s)	450, 473, 593, 694, 739, 1160, 1402, 1552, 1924
HpaI	GTTAAC	1 cut(s)	412
HpaII	CCGG	8 cut(s)	354, 467, 498, 625, 643, 806, 1171, 1886
HphI	GGTGA	5 cut(s)	220, 319, 361, 1280, 1436
Hpy166II	GTNNAC	7 cut(s)	316, 412, 481, 759, 942, 1276, 1609
Hpy188I	TCNGA	4 cut(s)	1102, 1147, 1159, 1401
Hpy8I	GTNNAC	7 cut(s)	316, 412, 481, 759, 942, 1276, 1609
Hpy99I	CGWCG	4 cut(s)	269, 272, 398, 437
HpyAV	CCTTC	9 cut(s)	31, 82, 103, 116, 440, 761, 1236, 1258, 1315
HpyCH4III	ACNGT	5 cut(s)	311, 459, 615, 843, 1860
HpyCH4IV	ACGT	4 cut(s)	267, 432, 1070, 1827
HpyCH4V	TGCA	9 cut(s)	599, 655, 716, 1221, 1358, 1442, 1531, 1663, 1772
HpyF10VI	GCNNNNNNNGC	4 cut(s)	404, 584, 652, 1862
HpyF3I	CTNAG	6 cut(s)	419, 588, 917, 936, 1156, 1400
HpySE526I	ACGT	4 cut(s)	267, 432, 1070, 1827
Hsp92I	GRCGYC	2 cut(s)	267, 1827
Hsp92II	CATG	5 cut(s)	722, 1505, 1606, 1747, 1763
HspAI	GCGC	2 cut(s)	13, 1256
KpnI	GGTACC	1 cut(s)	625
KroI	GCCGGC	1 cut(s)	642
KroNI	GCCGGC	1 cut(s)	644
KspAI	GTTAAC	1 cut(s)	412
Kzo9I	GATC	8 cut(s)	225, 913, 1029, 1123, 1426, 1477, 1570, 1891
LmnI	GCTCC	2 cut(s)	958, 1298
Lsp1109I	GCAGC	4 cut(s)	543, 658, 1675, 1912
LweI	GCATC	5 cut(s)	574, 703, 975, 1513, 1698
MabI	ACCWGGT	1 cut(s)	1420
MaeI	CTAG	9 cut(s)	462, 632, 707, 753, 1025, 1062, 1127, 1368, 1475
MaeII	ACGT	4 cut(s)	267, 432, 1070, 1827
MaeIII	GTNAC	4 cut(s)	280, 344, 367, 568
MalI	GATC	8 cut(s)	227, 915, 1031, 1125, 1428, 1479, 1572, 1893
MboI	GATC	8 cut(s)	225, 913, 1029, 1123, 1426, 1477, 1570, 1891
MflI	RGATCY	3 cut(s)	913, 1570, 1891
MhlI	GDGCHC	4 cut(s)	775, 792, 1753, 1867
MluCI	AATT	7 cut(s)	201, 504, 689, 1350, 1644, 1767, 1813
MluI	ACGCGT	1 cut(s)	277
MlyI	GAGTC	5 cut(s)	444, 467, 733, 1411, 1918
MmeI	TCCRAC	2 cut(s)	109, 233
Mph1103I	ATGCAT	2 cut(s)	657, 1223
MroNI	GCCGGC	1 cut(s)	642
MseI	TTAA	6 cut(s)	411, 503, 617, 996, 1133, 1464
MspI	CCGG	8 cut(s)	354, 467, 498, 625, 643, 806, 1171, 1886
MspR9I	CCNGG	5 cut(s)	365, 499, 807, 1172, 1422
Mva1269I	GAATGC	2 cut(s)	544, 655
MvaI	CCWGG	2 cut(s)	365, 1422
MvnI	CGCG	2 cut(s)	279, 1656
MwoI	GCNNNNNNNGC	4 cut(s)	404, 584, 652, 1862
NaeI	GCCGGC	1 cut(s)	644
NciI	CCSGG	3 cut(s)	499, 807, 1172
NdeI	CATATG	1 cut(s)	1774
NdeII	GATC	8 cut(s)	225, 913, 1029, 1123, 1426, 1477, 1570, 1891
NgoMIV	GCCGGC	1 cut(s)	642
NlaIII	CATG	5 cut(s)	722, 1505, 1606, 1747, 1763
NlaIV	GGNNCC	6 cut(s)	358, 623, 629, 1377, 1750, 1893
NmuCI	GTSAC	4 cut(s)	280, 344, 367, 568
NsbI	TGCGCA	1 cut(s)	14
NsiI	ATGCAT	2 cut(s)	657, 1223
NspV	TTCGAA	2 cut(s)	659, 697
PaeR7I	CTCGAG	4 cut(s)	425, 774, 793, 1730
PcsI	WCGNNNNNNNCGW	2 cut(s)	276, 432
PctI	GAATGC	2 cut(s)	544, 655
PdiI	GCCGGC	1 cut(s)	644
PfeI	GAWTC	4 cut(s)	593, 694, 1160, 1552
Pfl23II	CGTACG	1 cut(s)	454
PflFI	GACNNNGTC	1 cut(s)	476
PinAI	ACCGGT	2 cut(s)	353, 624
PkrI	GCNGC	8 cut(s)	248, 323, 517, 558, 648, 675, 1665, 1902
PleI	GAGTC	5 cut(s)	444, 467, 733, 1410, 1918
PpsI	GAGTC	5 cut(s)	444, 467, 733, 1410, 1918
Ppu21I	YACGTR	1 cut(s)	1071
PsiI	TTATAA	1 cut(s)	1650
Psp6I	CCWGG	2 cut(s)	363, 1420
PspEI	GGTNACC	1 cut(s)	367
PspFI	CCCAGC	1 cut(s)	1896
PspGI	CCWGG	2 cut(s)	363, 1420
PspLI	CGTACG	1 cut(s)	454
PspN4I	GGNNCC	6 cut(s)	358, 623, 629, 1377, 1750, 1893
PspPI	GGNCC	1 cut(s)	1375
PspXI	VCTCGAGB	1 cut(s)	425
PsrI	GAACNNNNNNTAC	2 cut(s)	1081, 1113
PstNI	CAGNNNCTG	1 cut(s)	1399
PsuI	RGATCY	3 cut(s)	913, 1570, 1891
PsyI	GACNNNGTC	1 cut(s)	476
RsaI	GTAC	3 cut(s)	431, 456, 623
RsaNI	GTAC	3 cut(s)	430, 455, 622
SaqAI	TTAA	6 cut(s)	411, 503, 617, 996, 1133, 1464
SatI	GCNGC	8 cut(s)	247, 322, 516, 557, 647, 674, 1664, 1901
Sau3AI	GATC	8 cut(s)	225, 913, 1029, 1123, 1426, 1477, 1570, 1891
Sau96I	GGNCC	1 cut(s)	1375
SchI	GAGTC	5 cut(s)	444, 467, 733, 1411, 1918
ScrFI	CCNGG	5 cut(s)	365, 499, 807, 1172, 1422
SduI	GDGCHC	4 cut(s)	775, 792, 1753, 1867
SexAI	ACCWGGT	1 cut(s)	1420
SfaNI	GCATC	5 cut(s)	574, 703, 975, 1513, 1698
Sfr274I	CTCGAG	4 cut(s)	425, 774, 793, 1730
SfuI	TTCGAA	2 cut(s)	659, 697
SinI	GGWCC	1 cut(s)	1375
SlaI	CTCGAG	4 cut(s)	425, 774, 793, 1730
SmlI	CTYRAG	4 cut(s)	425, 774, 793, 1730
SmoI	CTYRAG	4 cut(s)	425, 774, 793, 1730
SnaBI	TACGTA	1 cut(s)	1071
Sse9I	AATT	7 cut(s)	201, 504, 689, 1350, 1644, 1767, 1813
SspMI	CTAG	9 cut(s)	462, 632, 707, 753, 1025, 1062, 1127, 1368, 1475
StyD4I	CCNGG	5 cut(s)	363, 497, 805, 1170, 1420
StyI	CCWWGG	1 cut(s)	1061
TaaI	ACNGT	5 cut(s)	311, 459, 615, 843, 1860
TaiI	ACGT	4 cut(s)	270, 435, 1073, 1830
TaqI	TCGA	9 cut(s)	426, 659, 697, 775, 794, 904, 1480, 1731, 1873
TasI	AATT	7 cut(s)	201, 504, 689, 1350, 1644, 1767, 1813
TauI	GCSGC	4 cut(s)	249, 324, 518, 676
TfiI	GAWTC	4 cut(s)	593, 694, 1160, 1552
Tru1I	TTAA	6 cut(s)	411, 503, 617, 996, 1133, 1464
Tru9I	TTAA	6 cut(s)	411, 503, 617, 996, 1133, 1464
TscAI	CASTG	3 cut(s)	1449, 1549, 1865
TseFI	GTSAC	4 cut(s)	280, 344, 367, 568
TseI	GCWGC	4 cut(s)	556, 646, 1663, 1900
Tsp45I	GTSAC	4 cut(s)	280, 344, 367, 568
TspDTI	ATGAA	5 cut(s)	506, 924, 1014, 1296, 1518
TspGWI	ACGGA	3 cut(s)	332, 349, 466
TspRI	CASTG	3 cut(s)	1449, 1549, 1865
Tth111I	GACNNNGTC	1 cut(s)	476
VpaK11BI	GGWCC	1 cut(s)	1375
XapI	RAATTY	2 cut(s)	1350, 1644
XbaI	TCTAGA	5 cut(s)	461, 706, 1024, 1126, 1474
XhoI	CTCGAG	4 cut(s)	425, 774, 793, 1730
XmaJI	CCTAGG	1 cut(s)	1061
XmiI	GTMKAC	1 cut(s)	758
XspI	CTAG	9 cut(s)	462, 632, 707, 753, 1025, 1062, 1127, 1368, 1475
ZraI	GACGTC	2 cut(s)	268, 1828
Zsp2I	ATGCAT	2 cut(s)	657, 1223

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.