Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0013867

Physical Location & Seq

Genomic Coordinates Reverse (-)

20301621 .. 20302659

Gene Structure 1039 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1124400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 465 bp

ATGGGTGTTTTGGATCATTTTTCGAATCTATTTGATTGCTCAAGTGGGAGATCCAGGCTCAAGAAACGCAGGCAATTCCAGACGGTGGAGATCAAGGTGAAGATGGACTGCGAAGGATGCGAGCGGAAGGTGAAGAGATCGGTGGAGGGAATGAGGGGCGTGACGCAGGTGGACGTGGAGCGCAAGGCCCACAAGCTGACGGTCGTCGGATACGTGGACCCCGGCAAGGTTCTGGCACGTGTCGCGCATCGGACGGGGAAGAGGGTGGAGTTCTGGCCCTACGTCCCGTACGACGTCGTGGCGCACCCTTACGCCGCCGGAGTGTACGACAGGAAGGCGCCGTCCGGGTACGTGCGGAACGTGGAGGACCCGCAGATGGGGAACCTGGCACGTGCGAGCTCGACGGAAGTGCGTTACACCACGGCGTTCAGTGATGAAAACCCCGCTGCCTGCACTGTCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009506 GO:0009628 GO:0009987 GO:0010286 GO:0016020 GO:0019725 GO:0030001 GO:0030003 GO:0030054 GO:0032791 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046870 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0071944 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

155

Amino Acids

17.34

Weight (kDa)

9.46

Isoelectric Point (pI)

38.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	32 - 86	2.6e-12	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013867)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38580 AT4G38580
fragaria_vesca	FvH4_2g01160
malus_domestica	MD05G1121800.v1.1 MD10G1124400.v1.1
prunus_persica	Prupe.8G165600_v2.0.a1
pyrus_communis	pycom05g11580
rosa_chinensis	RchiOBHm_Chr6g0251781
rosa_laevigata	RLG00000015430
rosa_multiflora	Rmu_ssc0000213.1_g000113
rosa_roxburghii	Rroxscaffold_7G00216390
rosa_rugosa	Rorug05G0503900
rosa_samantha	Rh6AG014700 Rh6BG013100 Rh6CG010100 Rh6DG011300
rosa_wichuraiana	Rw6G001460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	157
AatII	GACGTC	1 cut(s)	297
Acc36I	ACCTGC	1 cut(s)	157
AccB1I	GGYRCC	1 cut(s)	337
AccB7I	CCANNNNNTGG	1 cut(s)	85
AccBSI	CCGCTC	1 cut(s)	124
AccII	CGCG	1 cut(s)	245
AciI	CCGC	5 cut(s)	124, 315, 355, 371, 444
AclWI	GGATC	2 cut(s)	21, 45
AcvI	CACGTG	2 cut(s)	239, 392
AcyI	GRCGYC	2 cut(s)	294, 338
AfaI	GTAC	3 cut(s)	290, 326, 350
AfiI	CCNNNNNNNGG	4 cut(s)	85, 226, 376, 377
AflIII	ACRYGT	1 cut(s)	238
AjiI	CACGTC	1 cut(s)	175
AjnI	CCWGG	2 cut(s)	53, 384
AluBI	AGCT	2 cut(s)	196, 399
AluI	AGCT	2 cut(s)	196, 399
Alw21I	GWGCWC	1 cut(s)	401
AlwI	GGATC	2 cut(s)	21, 45
AoxI	GGCC	2 cut(s)	186, 275
ApeKI	GCWGC	1 cut(s)	446
AspLEI	GCGC	4 cut(s)	183, 247, 304, 340
AspS9I	GGNCC	4 cut(s)	187, 217, 276, 367
AsuC2I	CCSGG	2 cut(s)	222, 346
AsuHPI	GGTGA	2 cut(s)	109, 142
AsuII	TTCGAA	1 cut(s)	23
AvaII	GGWCC	2 cut(s)	217, 367
BanI	GGYRCC	1 cut(s)	337
BanII	GRGCYC	1 cut(s)	401
BbrPI	CACGTG	2 cut(s)	239, 392
Bbv12I	GWGCWC	1 cut(s)	401
BbvI	GCAGC	1 cut(s)	433
BccI	CCATC	2 cut(s)	97, 370
BceAI	ACGGC	2 cut(s)	325, 438
BcgI	CGANNNNNNTGC	2 cut(s)	391, 425
BciT130I	CCWGG	2 cut(s)	55, 386
BciVI	GTATCC	1 cut(s)	203
BcnI	CCSGG	2 cut(s)	222, 346
BfoI	RGCGCY	1 cut(s)	341
BfuAI	ACCTGC	1 cut(s)	157
BfuI	GTATCC	1 cut(s)	203
BisI	GCNGC	2 cut(s)	315, 447
BlsI	GCNGC	2 cut(s)	316, 448
Bme1390I	CCNGG	4 cut(s)	55, 222, 346, 386
Bme18I	GGWCC	2 cut(s)	217, 367
BmgBI	CACGTC	1 cut(s)	175
BmgT120I	GGNCC	4 cut(s)	187, 217, 276, 367
BmiI	GGNNCC	4 cut(s)	219, 339, 369, 383
BmrFI	CCNGG	4 cut(s)	55, 222, 346, 386
BmsI	GCATC	2 cut(s)	107, 256
BoxI	GACNNNNGTC	1 cut(s)	203
Bpu14I	TTCGAA	1 cut(s)	23
BpuEI	CTTGAG	2 cut(s)	25, 44
BpuMI	CCSGG	2 cut(s)	222, 346
BsaAI	YACGTR	4 cut(s)	214, 239, 352, 392
BsaHI	GRCGYC	2 cut(s)	294, 338
BsaJI	CCNNGG	2 cut(s)	220, 420
Bsc4I	CCNNNNNNNGG	4 cut(s)	85, 226, 376, 377
BseBI	CCWGG	2 cut(s)	55, 386
BseDI	CCNNGG	2 cut(s)	220, 420
BseGI	GGATG	1 cut(s)	122
BseLI	CCNNNNNNNGG	4 cut(s)	85, 226, 376, 377
BseXI	GCAGC	1 cut(s)	433
BsgI	GTGCAG	1 cut(s)	436
Bsh1236I	CGCG	1 cut(s)	245
Bsh1285I	CGRYCG	1 cut(s)	204
BshFI	GGCC	2 cut(s)	188, 277
BshNI	GGYRCC	1 cut(s)	337
BsiEI	CGRYCG	1 cut(s)	204
BsiHKAI	GWGCWC	1 cut(s)	401
BsiSI	CCGG	3 cut(s)	222, 318, 345
BsiWI	CGTACG	1 cut(s)	288
BslFI	GGGAC	1 cut(s)	269
BslI	CCNNNNNNNGG	4 cut(s)	85, 226, 376, 377
BsmFI	GGGAC	1 cut(s)	269
BsnI	GGCC	2 cut(s)	188, 277
Bsp119I	TTCGAA	1 cut(s)	23
Bsp1286I	GDGCHC	1 cut(s)	401
Bsp143I	GATC	4 cut(s)	13, 50, 90, 137
BspACI	CCGC	5 cut(s)	124, 315, 355, 371, 444
BspANI	GGCC	2 cut(s)	188, 277
BspFNI	CGCG	1 cut(s)	245
BspLI	GGNNCC	4 cut(s)	219, 339, 369, 383
BspMI	ACCTGC	1 cut(s)	157
BspPI	GGATC	2 cut(s)	21, 45
BspT104I	TTCGAA	1 cut(s)	23
BspT107I	GGYRCC	1 cut(s)	337
BsrBI	CCGCTC	1 cut(s)	124
BssECI	CCNNGG	2 cut(s)	220, 420
BssMI	GATC	4 cut(s)	13, 50, 90, 137
BssNI	GRCGYC	2 cut(s)	294, 338
Bst2UI	CCWGG	2 cut(s)	55, 386
Bst4CI	ACNGT	3 cut(s)	85, 202, 457
Bst6I	CTCTTC	2 cut(s)	128, 254
BstACI	GRCGYC	2 cut(s)	294, 338
BstBAI	YACGTR	4 cut(s)	214, 239, 352, 392
BstBI	TTCGAA	1 cut(s)	23
BstC8I	GCNNGC	4 cut(s)	71, 122, 397, 451
BstDSI	CCRYGG	1 cut(s)	420
BstF5I	GGATG	1 cut(s)	122
BstFNI	CGCG	1 cut(s)	245
BstH2I	RGCGCY	1 cut(s)	341
BstHHI	GCGC	4 cut(s)	183, 247, 304, 340
BstKTI	GATC	4 cut(s)	16, 53, 93, 140
BstMBI	GATC	4 cut(s)	13, 50, 90, 137
BstMCI	CGRYCG	1 cut(s)	204
BstMWI	GCNNNNNNNGC	2 cut(s)	117, 242
BstNI	CCWGG	2 cut(s)	55, 386
BstPAI	GACNNNNGTC	1 cut(s)	203
BstSCI	CCNGG	4 cut(s)	53, 220, 344, 384
BstUI	CGCG	1 cut(s)	245
BstV1I	GCAGC	1 cut(s)	433
BstX2I	RGATCY	1 cut(s)	50
BstYI	RGATCY	1 cut(s)	50
BsuI	GTATCC	1 cut(s)	203
BsuRI	GGCC	2 cut(s)	188, 277
BtgI	CCRYGG	1 cut(s)	420
BtrI	CACGTC	1 cut(s)	175
BtsCI	GGATG	1 cut(s)	122
BtsIMutI	CAGTG	2 cut(s)	436, 453
BveI	ACCTGC	1 cut(s)	157
Cac8I	GCNNGC	4 cut(s)	71, 122, 397, 451
CfoI	GCGC	4 cut(s)	183, 247, 304, 340
Cfr13I	GGNCC	4 cut(s)	187, 217, 276, 367
CseI	GACGC	1 cut(s)	172
Csp6I	GTAC	3 cut(s)	289, 325, 349
CviAII	CATG	1 cut(s)	460
CviJI	RGCY	5 cut(s)	58, 188, 196, 277, 399
CviKI_1	RGCY	5 cut(s)	58, 188, 196, 277, 399
CviQI	GTAC	3 cut(s)	289, 325, 349
DinI	GGCGCC	1 cut(s)	339
DpnI	GATC	4 cut(s)	15, 52, 92, 139
DpnII	GATC	4 cut(s)	13, 50, 90, 137
Eam1104I	CTCTTC	2 cut(s)	128, 254
EarI	CTCTTC	2 cut(s)	128, 254
Ecl136II	GAGCTC	1 cut(s)	399
Eco24I	GRGCYC	1 cut(s)	401
Eco47I	GGWCC	2 cut(s)	217, 367
Eco53kI	GAGCTC	1 cut(s)	399
Eco72I	CACGTG	2 cut(s)	239, 392
EcoICRI	GAGCTC	1 cut(s)	399
EcoO109I	RGGNCCY	1 cut(s)	367
EcoRII	CCWGG	2 cut(s)	53, 384
EcoT38I	GRGCYC	1 cut(s)	401
EgeI	GGCGCC	1 cut(s)	339
EheI	GGCGCC	1 cut(s)	339
FaeI	CATG	1 cut(s)	463
FaiI	YATR	1 cut(s)	461
FaqI	GGGAC	1 cut(s)	269
FatI	CATG	1 cut(s)	459
FauI	CCCGC	2 cut(s)	378, 451
Fnu4HI	GCNGC	2 cut(s)	315, 447
FokI	GGATG	1 cut(s)	129
FriOI	GRGCYC	1 cut(s)	401
Fsp4HI	GCNGC	2 cut(s)	315, 447
GlaI	GCGC	4 cut(s)	182, 246, 303, 339
GluI	GCNGC	2 cut(s)	315, 447
HaeII	RGCGCY	1 cut(s)	341
HaeIII	GGCC	2 cut(s)	188, 277
HapII	CCGG	3 cut(s)	222, 318, 345
HgaI	GACGC	1 cut(s)	172
HhaI	GCGC	4 cut(s)	183, 247, 304, 340
Hin1I	GRCGYC	2 cut(s)	294, 338
Hin1II	CATG	1 cut(s)	463
Hin6I	GCGC	4 cut(s)	181, 245, 302, 338
HinP1I	GCGC	4 cut(s)	181, 245, 302, 338
HinfI	GANTC	1 cut(s)	25
HpaII	CCGG	3 cut(s)	222, 318, 345
HphI	GGTGA	2 cut(s)	109, 142
Hpy166II	GTNNAC	3 cut(s)	172, 217, 325
Hpy188I	TCNGA	2 cut(s)	209, 252
Hpy188III	TCNNGA	2 cut(s)	61, 79
Hpy8I	GTNNAC	3 cut(s)	172, 217, 325
Hpy99I	CGWCG	4 cut(s)	209, 296, 299, 406
HpyAV	CCTTC	3 cut(s)	107, 121, 328
HpyCH4III	ACNGT	3 cut(s)	85, 202, 457
HpyCH4IV	ACGT	8 cut(s)	174, 213, 238, 282, 294, 351, 360, 391
HpyCH4V	TGCA	1 cut(s)	453
HpyF10VI	GCNNNNNNNGC	2 cut(s)	117, 242
HpySE526I	ACGT	8 cut(s)	174, 213, 238, 282, 294, 351, 360, 391
Hsp92I	GRCGYC	2 cut(s)	294, 338
Hsp92II	CATG	1 cut(s)	463
HspAI	GCGC	4 cut(s)	181, 245, 302, 338
KasI	GGCGCC	1 cut(s)	337
Kzo9I	GATC	4 cut(s)	13, 50, 90, 137
LmnI	GCTCC	1 cut(s)	178
Lsp1109I	GCAGC	1 cut(s)	433
LweI	GCATC	2 cut(s)	107, 256
MaeII	ACGT	8 cut(s)	174, 213, 238, 282, 294, 351, 360, 391
MaeIII	GTNAC	2 cut(s)	160, 413
MalI	GATC	4 cut(s)	15, 52, 92, 139
MbiI	CCGCTC	1 cut(s)	124
MboI	GATC	4 cut(s)	13, 50, 90, 137
MboII	GAAGA	3 cut(s)	112, 145, 271
MflI	RGATCY	1 cut(s)	50
MhlI	GDGCHC	1 cut(s)	401
MluCI	AATT	1 cut(s)	74
Mly113I	GGCGCC	1 cut(s)	338
MmeI	TCCRAC	1 cut(s)	187
MnlI	CCTC	4 cut(s)	139, 147, 255, 358
MslI	CAYNNNNRTG	1 cut(s)	458
MspA1I	CMGCKG	1 cut(s)	446
MspI	CCGG	3 cut(s)	222, 318, 345
MspR9I	CCNGG	4 cut(s)	55, 222, 346, 386
MvaI	CCWGG	2 cut(s)	55, 386
MvnI	CGCG	1 cut(s)	245
MwoI	GCNNNNNNNGC	2 cut(s)	117, 242
NarI	GGCGCC	1 cut(s)	338
NciI	CCSGG	2 cut(s)	222, 346
NdeII	GATC	4 cut(s)	13, 50, 90, 137
NlaIII	CATG	1 cut(s)	463
NlaIV	GGNNCC	4 cut(s)	219, 339, 369, 383
NmuCI	GTSAC	1 cut(s)	160
NspV	TTCGAA	1 cut(s)	23
PaqCI	CACCTGC	1 cut(s)	157
PcsI	WCGNNNNNNNCGW	3 cut(s)	210, 288, 357
PfeI	GAWTC	1 cut(s)	25
Pfl23II	CGTACG	1 cut(s)	288
PflMI	CCANNNNNTGG	1 cut(s)	85
PkrI	GCNGC	2 cut(s)	316, 448
PluTI	GGCGCC	1 cut(s)	341
PmaCI	CACGTG	2 cut(s)	239, 392
PmlI	CACGTG	2 cut(s)	239, 392
Ppu21I	YACGTR	4 cut(s)	214, 239, 352, 392
PpuMI	RGGWCCY	1 cut(s)	367
PshAI	GACNNNNGTC	1 cut(s)	203
Psp124BI	GAGCTC	1 cut(s)	401
Psp5II	RGGWCCY	1 cut(s)	367
Psp6I	CCWGG	2 cut(s)	53, 384
PspCI	CACGTG	2 cut(s)	239, 392
PspGI	CCWGG	2 cut(s)	53, 384
PspLI	CGTACG	1 cut(s)	288
PspN4I	GGNNCC	4 cut(s)	219, 339, 369, 383
PspPI	GGNCC	4 cut(s)	187, 217, 276, 367
PspPPI	RGGWCCY	1 cut(s)	367
PsuI	RGATCY	1 cut(s)	50
RsaI	GTAC	3 cut(s)	290, 326, 350
RsaNI	GTAC	3 cut(s)	289, 325, 349
RseI	CAYNNNNRTG	1 cut(s)	458
SacI	GAGCTC	1 cut(s)	401
SatI	GCNGC	2 cut(s)	315, 447
Sau3AI	GATC	4 cut(s)	13, 50, 90, 137
Sau96I	GGNCC	4 cut(s)	187, 217, 276, 367
ScrFI	CCNGG	4 cut(s)	55, 222, 346, 386
SduI	GDGCHC	1 cut(s)	401
SfaNI	GCATC	2 cut(s)	107, 256
SfoI	GGCGCC	1 cut(s)	339
SfuI	TTCGAA	1 cut(s)	23
SinI	GGWCC	2 cut(s)	217, 367
SmiMI	CAYNNNNRTG	1 cut(s)	458
SmlI	CTYRAG	2 cut(s)	40, 59
SmoI	CTYRAG	2 cut(s)	40, 59
Sse9I	AATT	1 cut(s)	74
SsiI	CCGC	5 cut(s)	124, 315, 355, 371, 444
SspDI	GGCGCC	1 cut(s)	337
SstI	GAGCTC	1 cut(s)	401
StyD4I	CCNGG	4 cut(s)	53, 220, 344, 384
TaaI	ACNGT	3 cut(s)	85, 202, 457
TaiI	ACGT	8 cut(s)	177, 216, 241, 285, 297, 354, 363, 394
TaqI	TCGA	2 cut(s)	23, 401
TasI	AATT	1 cut(s)	74
TauI	GCSGC	1 cut(s)	317
TfiI	GAWTC	1 cut(s)	25
TscAI	CASTG	2 cut(s)	436, 460
TseFI	GTSAC	1 cut(s)	160
TseI	GCWGC	1 cut(s)	446
Tsp45I	GTSAC	1 cut(s)	160
TspDTI	ATGAA	1 cut(s)	450
TspGWI	ACGGA	1 cut(s)	419
TspRI	CASTG	2 cut(s)	436, 460
Van91I	CCANNNNNTGG	1 cut(s)	85
VpaK11BI	GGWCC	2 cut(s)	217, 367
ZraI	GACGTC	1 cut(s)	295

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.