MD10G1139000.v1.1

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015545
Physical Location & Seq
Forward (+)
22391395 .. 22394613
3219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1139000.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGAGAACTCGTCGACTCTGAGAGAGTGGTTCTCGCGAGTTGACGCGGCCAATACCGGAAACATCACAGCGCCTCAGCTCAAGAGCGCTCTAGCTGTTGGAAACCTCGACTTCCCTCTCTCAGTTGTCCAGCAAATGATCAGGATGTATGATTTTGATAGGAATGGGACTATGAGCTTTGAAGAGTTTCTTGCGCTTAACAAGTTCCTCCTCAAGGTTCAACAAGCCTTCTCGGACCGTGAGAGGGGACGTGGCTATCTTGTTACTGATGATGTGAATGAGGCATTGGTGAAAATCGGCTTCTTGCTGGACACTCCTGCTTTCTACACAGTCTGTGAGAGCTTTGATCAAAAGAAGAAAGGAATGTTCAGACTAGATGACTTCATATCTCTTTGTATATTTTTGCAGTCTGCTCGTAATCTGTTTAATTCATTTGATACAGCCAAGCAAGGCAGAGTGACTCTGGATCTCAACCAGTTTGTCTACTGCACTGCCAATTGCAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_7 EF-hand_8

Protein Analysis

170

Amino Acids

19.32

Weight (kDa)

5.5

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 6 - 65 2e-08 EF-hand domain pair
EF-hand_8 PF13833 19 - 65 4.1e-06 EF-hand domain pair
EF-hand_1 PF00036 43 - 66 5.3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015545)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g16570
malus_domestica MD10G1139000.v1.1
prunus_persica Prupe.8G177800_v2.0.a1
pyrus_communis pycom10g11820
rosa_chinensis RchiOBHm_Chr6g0280641
rosa_laevigata RLG00000013041
rosa_multiflora Rmu_sc0000976.1_g000003
rosa_roxburghii Rroxscaffold_7G00187850
rosa_rugosa Rorug06G0133300 Rorug06G0133300 Rorug06G0133300
rosa_samantha Rh6AG245700 Rh6BG248100 Rh6CG249500 Rh6DG240800
rosa_wichuraiana Rw6G021270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 14, 483
AccII CGCG 2 cut(s) 37, 47
AciI CCGC 1 cut(s) 47
AclWI GGATC 1 cut(s) 474
AcoI YGGCCR 1 cut(s) 48
AfeI AGCGCT 1 cut(s) 88
AfiI CCNNNNNNNGG 2 cut(s) 214, 244
AgsI TTSAA 2 cut(s) 182, 221
AjiI CACGTC 1 cut(s) 251
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AluBI AGCT 4 cut(s) 79, 95, 177, 342
AluI AGCT 4 cut(s) 79, 95, 177, 342
AlwI GGATC 1 cut(s) 474
Aor51HI AGCGCT 1 cut(s) 88
AoxI GGCC 1 cut(s) 48
Asp700I GAANNNNTTC 1 cut(s) 186
AspLEI GCGC 3 cut(s) 73, 89, 196
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 1 cut(s) 235
BbvCI CCTCAGC 1 cut(s) 75
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BclI TGATCA 2 cut(s) 138, 346
BfaI CTAG 2 cut(s) 92, 374
BfoI RGCGCY 2 cut(s) 74, 90
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
Bme18I GGWCC 1 cut(s) 235
BmgBI CACGTC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 235
BplI GAGNNNNNCTC 2 cut(s) 17, 49
Bpu10I CCTNAGC 1 cut(s) 75
BpuEI CTTGAG 2 cut(s) 65, 197
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 244
Bse1I ACTGG 1 cut(s) 475
BseGI GGATG 1 cut(s) 150
BseLI CCNNNNNNNGG 2 cut(s) 214, 244
BseMII CTCAG 3 cut(s) 11, 89, 135
BseNI ACTGG 1 cut(s) 475
BseRI GAGGAG 1 cut(s) 200
BsgI GTGCAG 1 cut(s) 472
Bsh1236I CGCG 2 cut(s) 37, 47
BshFI GGCC 1 cut(s) 50
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 2 cut(s) 181, 261
BslI CCNNNNNNNGG 2 cut(s) 214, 244
BsmFI GGGAC 2 cut(s) 181, 261
BsnI GGCC 1 cut(s) 50
Bsp143I GATC 3 cut(s) 138, 346, 466
Bsp68I TCGCGA 1 cut(s) 37
BspACI CCGC 1 cut(s) 47
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 3 cut(s) 12, 88, 134
BspFNI CGCG 2 cut(s) 37, 47
BspPI GGATC 1 cut(s) 474
BsrI ACTGG 1 cut(s) 475
BssMI GATC 3 cut(s) 138, 346, 466
Bst4CI ACNGT 2 cut(s) 239, 331
Bst6I CTCTTC 1 cut(s) 177
BstDEI CTNAG 3 cut(s) 20, 75, 121
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 150
BstFNI CGCG 2 cut(s) 37, 47
BstH2I RGCGCY 2 cut(s) 74, 90
BstHHI GCGC 3 cut(s) 73, 89, 196
BstKTI GATC 3 cut(s) 141, 349, 469
BstMBI GATC 3 cut(s) 138, 346, 466
BstUI CGCG 2 cut(s) 37, 47
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 1 cut(s) 50
BtrI CACGTC 1 cut(s) 251
BtsCI GGATG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
BtuMI TCGCGA 1 cut(s) 37
CfoI GCGC 3 cut(s) 73, 89, 196
Cfr13I GGNCC 1 cut(s) 235
CpoI CGGWCCG 1 cut(s) 235
CseI GACGC 1 cut(s) 53
CspI CGGWCCG 1 cut(s) 235
CviJI RGCY 9 cut(s) 50, 79, 95, 177, 227, 255, 300, 342, 443
CviKI_1 RGCY 9 cut(s) 50, 79, 95, 177, 227, 255, 300, 342, 443
DdeI CTNAG 3 cut(s) 20, 75, 121
DpnI GATC 3 cut(s) 140, 348, 468
DpnII GATC 3 cut(s) 138, 346, 466
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 235
Eco47III AGCGCT 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 212
FaiI YATR 5 cut(s) 150, 173, 386, 398, 508
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 2 cut(s) 181, 261
FbaI TGATCA 2 cut(s) 138, 346
FblI GTMKAC 2 cut(s) 14, 483
Fnu4HI GCNGC 1 cut(s) 48
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 2 cut(s) 92, 374
GlaI GCGC 3 cut(s) 72, 88, 195
GluI GCNGC 1 cut(s) 48
HaeII RGCGCY 2 cut(s) 74, 90
HaeIII GGCC 1 cut(s) 50
HapII CCGG 1 cut(s) 57
HgaI GACGC 1 cut(s) 53
HhaI GCGC 3 cut(s) 73, 89, 196
Hin6I GCGC 3 cut(s) 71, 87, 194
HinP1I GCGC 3 cut(s) 71, 87, 194
HincII GTYRAC 2 cut(s) 15, 43
HindII GTYRAC 2 cut(s) 15, 43
HinfI GANTC 2 cut(s) 16, 460
HpaII CCGG 1 cut(s) 57
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 3 cut(s) 15, 43, 484
Hpy188I TCNGA 3 cut(s) 21, 235, 371
Hpy188III TCNNGA 4 cut(s) 36, 82, 142, 464
Hpy8I GTNNAC 3 cut(s) 15, 43, 484
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 2 cut(s) 239, 331
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 3 cut(s) 406, 489, 501
HpyF3I CTNAG 3 cut(s) 20, 75, 121
HpySE526I ACGT 1 cut(s) 250
HspAI GCGC 3 cut(s) 71, 87, 194
Ksp22I TGATCA 2 cut(s) 138, 346
Kzo9I GATC 3 cut(s) 138, 346, 466
LpnPI CCDG 7 cut(s) 70, 127, 143, 293, 330, 449, 488
MaeI CTAG 2 cut(s) 92, 374
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 2 cut(s) 262, 457
MalI GATC 3 cut(s) 140, 348, 468
MboI GATC 3 cut(s) 138, 346, 466
MboII GAAGA 2 cut(s) 194, 367
MfeI CAATTG 1 cut(s) 496
MflI RGATCY 1 cut(s) 466
MluCI AATT 2 cut(s) 427, 496
MlyI GAGTC 2 cut(s) 10, 454
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 7 cut(s) 84, 116, 126, 218, 221, 237, 274
MroXI GAANNNNTTC 1 cut(s) 186
MseI TTAA 2 cut(s) 198, 426
MspI CCGG 1 cut(s) 57
MunI CAATTG 1 cut(s) 496
MvnI CGCG 2 cut(s) 37, 47
NdeII GATC 3 cut(s) 138, 346, 466
NmuCI GTSAC 1 cut(s) 457
NruI TCGCGA 1 cut(s) 37
PdmI GAANNNNTTC 1 cut(s) 186
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 2 cut(s) 10, 454
PpsI GAGTC 2 cut(s) 10, 454
PspPI GGNCC 1 cut(s) 235
PsuI RGATCY 1 cut(s) 466
RruI TCGCGA 1 cut(s) 37
Rsr2I CGGWCCG 1 cut(s) 235
RsrII CGGWCCG 1 cut(s) 235
SalI GTCGAC 1 cut(s) 13
SaqAI TTAA 2 cut(s) 198, 426
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 3 cut(s) 138, 346, 466
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 10, 454
SetI ASST 7 cut(s) 81, 97, 108, 179, 219, 253, 344
SinI GGWCC 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 80, 212
SmoI CTYRAG 2 cut(s) 80, 212
Sse9I AATT 2 cut(s) 427, 496
SsiI CCGC 1 cut(s) 47
SspMI CTAG 2 cut(s) 92, 374
TaaI ACNGT 2 cut(s) 239, 331
TaiI ACGT 1 cut(s) 253
TaqI TCGA 2 cut(s) 14, 108
TasI AATT 2 cut(s) 427, 496
TauI GCSGC 1 cut(s) 50
Tru1I TTAA 2 cut(s) 198, 426
Tru9I TTAA 2 cut(s) 198, 426
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 2 cut(s) 373, 420
TspRI CASTG 1 cut(s) 496
VpaK11BI GGWCC 1 cut(s) 235
XagI CCTNNNNNAGG 1 cut(s) 212
XmiI GTMKAC 2 cut(s) 14, 483
XmnI GAANNNNTTC 1 cut(s) 186
XspI CTAG 2 cut(s) 92, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.