Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0015166

Physical Location & Seq

Genomic Coordinates Forward (+)

22777634 .. 22778743

Gene Structure 1110 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1142000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGCGATCTGCCACCAATCTCCGTTCAAGCTCTTCGCCGCAATCCCATCACCATCATCGAAGCCCAAACCCCTCCACCTCCGCTGCAACCTCTCCCTCCCCTCCAGACCTCCCCTCACCTCCACCGAGCCCGAACCCGTCTTCACTTCCGTCAAGGCCTTCGCCCCCGCCACCATCGCCAATCTCGGCCCCGGCTTCGACTTCTTGGGCTGCGCCGTCGACGGCCTCGGCGACTTCGTCTCTCTCACCCTCGACCCCCAGGTATCGCCGGGCGAGATCTCCATTTCCGAGATCTCCGGTGACCACAACTCCAAGAAGCTCAGCAAGAACCCTCTCTGGAACTGCGCCGGAATCGCTGCCATCGAAGTCATGAAGATGCTCGGAGTCCGATCGGTGGGCCTTTCCCTCACTCTGGAAAAGGGCTTGCCTTTGGGCTCCGGGCTCGGATCCAGTGCGGCGAGCGCGGCCGCCGCGGCTGTTGCGGTCAACGAAATTTTTGGCGGGAAATTGGGTATAGAGGAATTGGTGATCGCGGGGCTGAAATCGGAGGAGAAGGTTTCCGGTTACCACGCTGATAACATAGCTCCGGCTATTATGGGCGGGTTCGTATTGATTCGGAGCTATGAACCGTTGGATTTGATTCCGCTGATCTTTCCGGATGGTAAAGAATTGTTTTTTGTGTTGGCTACGCCGGAGTTTGAAGCTCCGACGAAGAAGATGAGAGCGGCGCTGCCTGCGGAGGTGGGGATGGCCCATCATGTCTGGAATTCGAGCCAGGCCGGGGCCCTTGTTGCGGCAGTGTTGCAGGGGGACTTGCCCGGGCTCGGCAGGGCTTTGTCGAGCGATAAGATCGTGGAGCCGCGGCGAGCTCCGCTGATTCCCGGTATGGACGCTGTAAAAAAGGCAGCAATCGAGGCCGGGGCATTCGGGTGCACCATTAGCGGGGCAGGTCCCACTGCCGTGGCGGTCACGGATGATCCCGAGAAAGGAAAGATGATCGGAGAAAAAATGGTGGCGGCCTTTCTAAAGGAGGGAAAGTTGAAGGCCGCGGCGAGTGTGAGTAGACTCGACCGGGTTGGCGCCAGACTTGTCAGCACCATTCCGAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0004413 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006555 GO:0006566 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009069 GO:0009086 GO:0009088 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019202 GO:0019752 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko01100 ko01110 ko01120 ko01230 map00260 map01100 map01110 map01120 map01230

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N

Protein Analysis

370

Amino Acids

38.19

Weight (kDa)

7.65

Isoelectric Point (pI)

45.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GHMP_kinases_N	PF00288	121 - 201	7.8e-16	GHMP kinases N terminal domain
GHMP_kinases_C	PF08544	264 - 338	5.5e-12	GHMP kinases C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17265
fragaria_vesca	FvH4_2g16830
malus_domestica	MD10G1142000.v1.1
prunus_persica	Prupe.8G180500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0280961
rosa_laevigata	RLG00000013012
rosa_multiflora	Rmu_sc0006173.1_g000036 Rmu_sc0020792.1_g000001 Rmu_sc0030424.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187450
rosa_rugosa	Rorug06G0137600
rosa_samantha	Rh6AG249600 Rh6BG251800 Rh6CG252300 Rh6DG244000
rosa_wichuraiana	Rw6G021590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	938
AccB1I	GGYRCC	1 cut(s)	1079
AccBSI	CCGCTC	1 cut(s)	725
AccI	GTMKAC	2 cut(s)	219, 1063
AccII	CGCG	5 cut(s)	464, 473, 533, 862, 1049
AccIII	TCCGGA	1 cut(s)	655
AclWI	GGATC	3 cut(s)	441, 454, 971
AcoI	YGGCCR	1 cut(s)	465
AcsI	RAATTY	2 cut(s)	492, 766
AcyI	GRCGYC	1 cut(s)	1080
AfiI	CCNNNNNNNGG	7 cut(s)	394, 412, 781, 793, 824, 942, 986
AgsI	TTSAA	3 cut(s)	28, 701, 1042
AjnI	CCWGG	2 cut(s)	258, 774
AleI	CACNNNNGTG	1 cut(s)	959
AluBI	AGCT	6 cut(s)	31, 319, 584, 621, 704, 869
AluI	AGCT	6 cut(s)	31, 319, 584, 621, 704, 869
Alw21I	GWGCWC	2 cut(s)	871, 935
Alw26I	GTCTC	1 cut(s)	244
Alw44I	GTGCAC	1 cut(s)	931
AlwI	GGATC	3 cut(s)	441, 454, 971
Ama87I	CYCGRG	2 cut(s)	818, 980
Aor13HI	TCCGGA	1 cut(s)	655
ApaI	GGGCCC	1 cut(s)	787
ApaLI	GTGCAC	1 cut(s)	931
ApeKI	GCWGC	5 cut(s)	84, 210, 356, 730, 905
ApoI	RAATTY	2 cut(s)	492, 766
AspLEI	GCGC	5 cut(s)	215, 347, 464, 730, 1082
AspS9I	GGNCC	6 cut(s)	188, 397, 751, 783, 784, 950
AsuC2I	CCSGG	9 cut(s)	192, 270, 439, 781, 819, 820, 882, 919, 1073
AsuHPI	GGTGA	5 cut(s)	42, 109, 238, 311, 538
AvaI	CYCGRG	2 cut(s)	818, 980
AvaII	GGWCC	1 cut(s)	950
BaeGI	GKGCMC	2 cut(s)	787, 935
BamHI	GGATCC	1 cut(s)	446
BanI	GGYRCC	1 cut(s)	1079
BanII	GRGCYC	6 cut(s)	132, 437, 444, 787, 825, 871
BbsI	GAAGAC	1 cut(s)	133
Bbv12I	GWGCWC	2 cut(s)	871, 935
BbvI	GCAGC	5 cut(s)	71, 197, 343, 717, 917
BccI	CCATC	7 cut(s)	55, 61, 182, 368, 653, 742, 762
BceAI	ACGGC	3 cut(s)	200, 238, 944
BciT130I	CCWGG	2 cut(s)	260, 776
BcnI	CCSGG	9 cut(s)	192, 270, 439, 781, 819, 820, 882, 919, 1073
BcoDI	GTCTC	1 cut(s)	244
BfoI	RGCGCY	2 cut(s)	731, 1083
BfuAI	ACCTGC	1 cut(s)	938
BglI	GCCNNNNNGGC	1 cut(s)	473
BglII	AGATCT	2 cut(s)	276, 291
BlpI	GCTNAGC	1 cut(s)	320
Bme18I	GGWCC	1 cut(s)	950
BmeT110I	CYCGRG	2 cut(s)	818, 980
BmgT120I	GGNCC	6 cut(s)	188, 397, 751, 783, 784, 950
BmiI	GGNNCC	8 cut(s)	190, 436, 448, 784, 785, 858, 952, 1081
BmsI	GCATC	1 cut(s)	366
BpiI	GAAGAC	1 cut(s)	133
BpmI	CTGGAG	1 cut(s)	88
Bpu1102I	GCTNAGC	1 cut(s)	320
BpuMI	CCSGG	9 cut(s)	192, 270, 439, 781, 819, 820, 882, 919, 1073
BsaHI	GRCGYC	1 cut(s)	1080
BsaWI	WCCGGW	3 cut(s)	296, 560, 655
BsaXI	ACNNNNNCTCC	4 cut(s)	293, 323, 1022, 1052
Bsc4I	CCNNNNNNNGG	7 cut(s)	394, 412, 781, 793, 824, 942, 986
Bse1I	ACTGG	1 cut(s)	450
BseAI	TCCGGA	1 cut(s)	655
BseBI	CCWGG	2 cut(s)	260, 776
BseGI	GGATG	3 cut(s)	664, 753, 979
BseLI	CCNNNNNNNGG	7 cut(s)	394, 412, 781, 793, 824, 942, 986
BseMII	CTCAG	1 cut(s)	334
BseNI	ACTGG	1 cut(s)	450
BseRI	GAGGAG	1 cut(s)	563
BseSI	GKGCMC	2 cut(s)	787, 935
BseX3I	CGGCCG	1 cut(s)	465
BseXI	GCAGC	5 cut(s)	71, 197, 343, 717, 917
Bsh1236I	CGCG	5 cut(s)	464, 473, 533, 862, 1049
Bsh1285I	CGRYCG	3 cut(s)	392, 468, 1072
BshNI	GGYRCC	1 cut(s)	1079
BsiEI	CGRYCG	3 cut(s)	392, 468, 1072
BsiHKAI	GWGCWC	2 cut(s)	871, 935
BsiHKCI	CYCGRG	2 cut(s)	818, 980
BslFI	GGGAC	2 cut(s)	824, 936
BslI	CCNNNNNNNGG	7 cut(s)	394, 412, 781, 793, 824, 942, 986
BsmAI	GTCTC	1 cut(s)	244
BsmBI	CGTCTC	1 cut(s)	244
BsmFI	GGGAC	2 cut(s)	824, 936
BsmI	GAATGC	1 cut(s)	923
BsoBI	CYCGRG	2 cut(s)	818, 980
Bsp120I	GGGCCC	1 cut(s)	783
Bsp1286I	GDGCHC	7 cut(s)	132, 437, 444, 787, 825, 871, 935
Bsp13I	TCCGGA	1 cut(s)	655
Bsp1720I	GCTNAGC	1 cut(s)	320
BspCNI	CTCAG	1 cut(s)	333
BspEI	TCCGGA	1 cut(s)	655
BspFNI	CGCG	5 cut(s)	464, 473, 533, 862, 1049
BspHI	TCATGA	1 cut(s)	369
BspLI	GGNNCC	8 cut(s)	190, 436, 448, 784, 785, 858, 952, 1081
BspMI	ACCTGC	1 cut(s)	938
BspPI	GGATC	3 cut(s)	441, 454, 971
BspQI	GCTCTTC	1 cut(s)	38
BspT107I	GGYRCC	1 cut(s)	1079
BsrBI	CCGCTC	1 cut(s)	725
BsrI	ACTGG	1 cut(s)	450
BssNI	GRCGYC	1 cut(s)	1080
Bst2UI	CCWGG	2 cut(s)	260, 776
Bst4CI	ACNGT	1 cut(s)	630
Bst6I	CTCTTC	1 cut(s)	38
BstACI	GRCGYC	1 cut(s)	1080
BstC8I	GCNNGC	4 cut(s)	425, 460, 735, 867
BstDEI	CTNAG	1 cut(s)	320
BstDSI	CCRYGG	4 cut(s)	471, 860, 960, 1047
BstEII	GGTNACC	2 cut(s)	299, 563
BstF5I	GGATG	3 cut(s)	664, 753, 979
BstFNI	CGCG	5 cut(s)	464, 473, 533, 862, 1049
BstH2I	RGCGCY	2 cut(s)	731, 1083
BstHHI	GCGC	5 cut(s)	215, 347, 464, 730, 1082
BstMAI	GTCTC	1 cut(s)	244
BstMCI	CGRYCG	3 cut(s)	392, 468, 1072
BstNI	CCWGG	2 cut(s)	260, 776
BstPI	GGTNACC	2 cut(s)	299, 563
BstSLI	GKGCMC	2 cut(s)	787, 935
BstUI	CGCG	5 cut(s)	464, 473, 533, 862, 1049
BstV1I	GCAGC	5 cut(s)	71, 197, 343, 717, 917
BstV2I	GAAGAC	1 cut(s)	133
BstX2I	RGATCY	3 cut(s)	276, 291, 446
BstXI	CCANNNNNNTGG	1 cut(s)	961
BstYI	RGATCY	3 cut(s)	276, 291, 446
BstZI	CGGCCG	1 cut(s)	465
BtgI	CCRYGG	4 cut(s)	471, 860, 960, 1047
BtgZI	GCGATG	1 cut(s)	160
BtsCI	GGATG	3 cut(s)	664, 753, 979
BtsI	GCAGTG	2 cut(s)	804, 954
BtsIMutI	CAGTG	3 cut(s)	457, 804, 954
BveI	ACCTGC	1 cut(s)	938
Cac8I	GCNNGC	4 cut(s)	425, 460, 735, 867
CciI	TCATGA	1 cut(s)	369
CciNI	GCGGCCGC	1 cut(s)	465
CfoI	GCGC	5 cut(s)	215, 347, 464, 730, 1082
Cfr13I	GGNCC	6 cut(s)	188, 397, 751, 783, 784, 950
Cfr42I	CCGCGG	3 cut(s)	474, 863, 1050
Cfr9I	CCCGGG	1 cut(s)	818
CseI	GACGC	1 cut(s)	899
CviAII	CATG	2 cut(s)	370, 758
DdeI	CTNAG	1 cut(s)	320
DinI	GGCGCC	1 cut(s)	1081
EaeI	YGGCCR	1 cut(s)	465
EagI	CGGCCG	1 cut(s)	465
Eam1104I	CTCTTC	1 cut(s)	38
EarI	CTCTTC	1 cut(s)	38
Ecl136II	GAGCTC	1 cut(s)	869
EclXI	CGGCCG	1 cut(s)	465
Eco147I	AGGCCT	1 cut(s)	158
Eco24I	GRGCYC	6 cut(s)	132, 437, 444, 787, 825, 871
Eco47I	GGWCC	1 cut(s)	950
Eco52I	CGGCCG	1 cut(s)	465
Eco53kI	GAGCTC	1 cut(s)	869
Eco88I	CYCGRG	2 cut(s)	818, 980
Eco91I	GGTNACC	2 cut(s)	299, 563
EcoICRI	GAGCTC	1 cut(s)	869
EcoO109I	RGGNCCY	3 cut(s)	783, 784, 950
EcoO65I	GGTNACC	2 cut(s)	299, 563
EcoRI	GAATTC	1 cut(s)	766
EcoRII	CCWGG	2 cut(s)	258, 774
EcoT38I	GRGCYC	6 cut(s)	132, 437, 444, 787, 825, 871
EgeI	GGCGCC	1 cut(s)	1081
EheI	GGCGCC	1 cut(s)	1081
Esp3I	CGTCTC	1 cut(s)	244
FaeI	CATG	2 cut(s)	373, 761
FaiI	YATR	7 cut(s)	371, 515, 581, 596, 624, 759, 887
FaqI	GGGAC	2 cut(s)	824, 936
FatI	CATG	2 cut(s)	369, 757
FauI	CCCGC	5 cut(s)	175, 494, 526, 593, 935
FblI	GTMKAC	2 cut(s)	219, 1063
FokI	GGATG	3 cut(s)	671, 760, 986
FriOI	GRGCYC	6 cut(s)	132, 437, 444, 787, 825, 871
GlaI	GCGC	5 cut(s)	214, 346, 463, 729, 1081
GsuI	CTGGAG	1 cut(s)	88
HaeII	RGCGCY	2 cut(s)	731, 1083
HgaI	GACGC	1 cut(s)	899
HhaI	GCGC	5 cut(s)	215, 347, 464, 730, 1082
Hin1I	GRCGYC	1 cut(s)	1080
Hin1II	CATG	2 cut(s)	373, 761
Hin6I	GCGC	5 cut(s)	213, 345, 462, 728, 1080
HinP1I	GCGC	5 cut(s)	213, 345, 462, 728, 1080
HincII	GTYRAC	2 cut(s)	220, 487
HindII	GTYRAC	2 cut(s)	220, 487
HinfI	GANTC	6 cut(s)	351, 384, 613, 640, 877, 1065
HphI	GGTGA	5 cut(s)	42, 109, 238, 311, 538
Hpy166II	GTNNAC	4 cut(s)	220, 487, 933, 1064
Hpy188I	TCNGA	9 cut(s)	289, 383, 389, 446, 547, 618, 708, 1001, 1104
Hpy188III	TCNNGA	7 cut(s)	105, 337, 370, 413, 656, 763, 980
Hpy8I	GTNNAC	4 cut(s)	220, 487, 933, 1064
Hpy99I	CGWCG	3 cut(s)	221, 224, 712
HpyAV	CCTTC	3 cut(s)	169, 547, 1036
HpyCH4III	ACNGT	1 cut(s)	630
HpyCH4V	TGCA	3 cut(s)	87, 805, 933
HpyF3I	CTNAG	1 cut(s)	320
Hsp92I	GRCGYC	1 cut(s)	1080
Hsp92II	CATG	2 cut(s)	373, 761
HspAI	GCGC	5 cut(s)	213, 345, 462, 728, 1080
KasI	GGCGCC	1 cut(s)	1079
Kpn2I	TCCGGA	1 cut(s)	655
KspI	CCGCGG	3 cut(s)	474, 863, 1050
LguI	GCTCTTC	1 cut(s)	38
LmnI	GCTCC	6 cut(s)	440, 589, 618, 709, 856, 874
Lsp1109I	GCAGC	5 cut(s)	71, 197, 343, 717, 917
LweI	GCATC	1 cut(s)	366
MaeIII	GTNAC	3 cut(s)	299, 563, 967
MbiI	CCGCTC	1 cut(s)	725
MboII	GAAGA	5 cut(s)	25, 133, 385, 724, 727
MflI	RGATCY	3 cut(s)	276, 291, 446
MhlI	GDGCHC	7 cut(s)	132, 437, 444, 787, 825, 871, 935
MluCI	AATT	5 cut(s)	492, 506, 521, 668, 766
Mly113I	GGCGCC	1 cut(s)	1080
MlyI	GAGTC	2 cut(s)	393, 1059
MmeI	TCCRAC	2 cut(s)	612, 731
MroI	TCCGGA	1 cut(s)	655
MslI	CAYNNNNRTG	3 cut(s)	374, 928, 959
MspA1I	CMGCKG	6 cut(s)	84, 473, 646, 862, 874, 1049
Mva1269I	GAATGC	1 cut(s)	923
MvaI	CCWGG	2 cut(s)	260, 776
MvnI	CGCG	5 cut(s)	464, 473, 533, 862, 1049
NarI	GGCGCC	1 cut(s)	1080
NciI	CCSGG	9 cut(s)	192, 270, 439, 781, 819, 820, 882, 919, 1073
NlaIII	CATG	2 cut(s)	373, 761
NlaIV	GGNNCC	8 cut(s)	190, 436, 448, 784, 785, 858, 952, 1081
NmeAIII	GCCGAG	3 cut(s)	165, 207, 804
NmuCI	GTSAC	2 cut(s)	299, 967
NotI	GCGGCCGC	1 cut(s)	465
OliI	CACNNNNGTG	1 cut(s)	959
PagI	TCATGA	1 cut(s)	369
PceI	AGGCCT	1 cut(s)	158
PciSI	GCTCTTC	1 cut(s)	38
PcsI	WCGNNNNNNNCGW	3 cut(s)	228, 234, 360
PctI	GAATGC	1 cut(s)	923
PfeI	GAWTC	4 cut(s)	351, 613, 640, 877
PflFI	GACNNNGTC	1 cut(s)	236
Ple19I	CGATCG	1 cut(s)	392
PleI	GAGTC	2 cut(s)	392, 1059
PluTI	GGCGCC	1 cut(s)	1083
PpsI	GAGTC	2 cut(s)	392, 1059
PpuMI	RGGWCCY	1 cut(s)	950
Psp124BI	GAGCTC	1 cut(s)	871
Psp5II	RGGWCCY	1 cut(s)	950
Psp6I	CCWGG	2 cut(s)	258, 774
PspEI	GGTNACC	2 cut(s)	299, 563
PspGI	CCWGG	2 cut(s)	258, 774
PspN4I	GGNNCC	8 cut(s)	190, 436, 448, 784, 785, 858, 952, 1081
PspOMI	GGGCCC	1 cut(s)	783
PspPI	GGNCC	6 cut(s)	188, 397, 751, 783, 784, 950
PspPPI	RGGWCCY	1 cut(s)	950
PsuI	RGATCY	3 cut(s)	276, 291, 446
PsyI	GACNNNGTC	1 cut(s)	236
PvuI	CGATCG	1 cut(s)	392
RseI	CAYNNNNRTG	3 cut(s)	374, 928, 959
SacI	GAGCTC	1 cut(s)	871
SacII	CCGCGG	3 cut(s)	474, 863, 1050
SalI	GTCGAC	1 cut(s)	218
SapI	GCTCTTC	1 cut(s)	38
Sau96I	GGNCC	6 cut(s)	188, 397, 751, 783, 784, 950
SchI	GAGTC	2 cut(s)	393, 1059
SduI	GDGCHC	7 cut(s)	132, 437, 444, 787, 825, 871, 935
SfaNI	GCATC	1 cut(s)	366
SfoI	GGCGCC	1 cut(s)	1081
Sfr303I	CCGCGG	3 cut(s)	474, 863, 1050
SgrBI	CCGCGG	3 cut(s)	474, 863, 1050
SgrDI	CGTCGACG	1 cut(s)	218
SinI	GGWCC	1 cut(s)	950
SmaI	CCCGGG	1 cut(s)	820
SmiMI	CAYNNNNRTG	3 cut(s)	374, 928, 959
SrfI	GCCCGGGC	1 cut(s)	820
Sse9I	AATT	5 cut(s)	492, 506, 521, 668, 766
SseBI	AGGCCT	1 cut(s)	158
SspDI	GGCGCC	1 cut(s)	1079
SstI	GAGCTC	1 cut(s)	871
StuI	AGGCCT	1 cut(s)	158
TaaI	ACNGT	1 cut(s)	630
TaqI	TCGA	9 cut(s)	59, 198, 219, 252, 363, 770, 839, 912, 1068
TasI	AATT	5 cut(s)	492, 506, 521, 668, 766
TfiI	GAWTC	4 cut(s)	351, 613, 640, 877
TscAI	CASTG	3 cut(s)	457, 804, 961
TseFI	GTSAC	2 cut(s)	299, 967
TseI	GCWGC	5 cut(s)	84, 210, 356, 730, 905
Tsp45I	GTSAC	2 cut(s)	299, 967
TspDTI	ATGAA	2 cut(s)	386, 639
TspGWI	ACGGA	3 cut(s)	12, 139, 986
TspMI	CCCGGG	1 cut(s)	818
TspRI	CASTG	3 cut(s)	457, 804, 961
Tth111I	GACNNNGTC	1 cut(s)	236
VneI	GTGCAC	1 cut(s)	931
VpaK11BI	GGWCC	1 cut(s)	950
XapI	RAATTY	2 cut(s)	492, 766
XmaI	CCCGGG	1 cut(s)	818
XmiI	GTMKAC	2 cut(s)	219, 1063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.