Rorug06G0137600

homoserine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015166
Physical Location & Seq
Forward (+)
19474181 .. 19475456
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0137600.1

Sequence Viewer

Length: 1095 bp
ATGGATGGTGATGAAATCAAAAATTTCATCAAGGTATGGATCATAGCCATTGCATCTCAATGTTATTGTTATTACGTAGCAGCAAGAATCCCAACAAAGAGCATGATGAGGCTCATCTCTCTCCTCCCAATCTTGTACCTACTTCTCATGCTCCCCTTGAACCTCCACTCGATCCATCTTGGTGCCCCCTCAGCCTTCTTTCTTGGTTGTCTTGCCATCTTCAAACTCCTCCTCTTCTCCTTTGACTCTGGTCCTCTATCACCACCCCCAAAAGGACTTCTCCCCTTCATTTCAATAGCTCTTCTTCCCATCAACATTAAGAGACTCCCCAAAAGGCCTCGCTACGGTTACACTATCACAAAATTGGTGCCAAACAAGACAGCTTTGTGGGCATTGAAGGCCTTGCTTTTGGGTGAAATAATTCATGTACGCAAGGATTATAGACAAAATTTACATCCCTATGTACTCACATCCTTGTCCCTTTCCCACCTCTACCTTGGTCTCGAAATAGGCTTTGCTCTGAGTGTAGCCTTGCCTCAAGCCATGTTTGGGTTCGAACTCGAGCCACACTTCAACGAACCTTACTTTTCCACCTCTCTTCAGGACTTTTGGGGCCGTAGATGGAACCTTGCAGTCTCAAACACTCTACGCCCCCTTGTATACCATCCCATAAGGCGTATATCTACAGGCAAGAGCGGAGCCACCTACGAGCCAATAGATACCTCAAAACCTCCGCGGGCTCCAGTTCTGCCGGTCATCTCTGCTTTCACCATCTCGGGTCTAATGCACGAGCTCTTCTTTTACTACGTCACTCGTGCGCGTCCCACGGGGGAAATGATGTGCTTCTTTGTCCTACAAGGTGTGTGCTTGGCAATTGAACTAGAGGTGAAGAAGGCGTTGGCTCATAGGGTACGGTTTCACCCGCTAGTTTCAGGGCTGTTGACGTTGGTGTTTTTGATTGCCACTACTGATTGGCTTCTTTTCCCTCACATAATCAGAACTGGTGCGGATGCAAAGAGTTTAGAAGAGTGTGCCATTATGGTGGATTTTGTGAAGGCCAACATTTCCCCATTGTATTATTGGCAGAAGAGTTAG

Protein Analysis

364

Amino Acids

41.21

Weight (kDa)

9.51

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 188 - 284 1.6e-16 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015166)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17265
fragaria_vesca FvH4_2g16830
malus_domestica MD10G1142000.v1.1
prunus_persica Prupe.8G180500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0280961
rosa_laevigata RLG00000013012
rosa_multiflora Rmu_sc0006173.1_g000036 Rmu_sc0020792.1_g000001 Rmu_sc0030424.1_g000001
rosa_roxburghii Rroxscaffold_7G00187450
rosa_rugosa Rorug06G0137600
rosa_samantha Rh6AG249600 Rh6BG251800 Rh6CG252300 Rh6DG244000
rosa_wichuraiana Rw6G021590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 182, 367
AccBSI CCGCTC 1 cut(s) 696
AccI GTMKAC 1 cut(s) 660
AccII CGCG 2 cut(s) 736, 820
AciI CCGC 5 cut(s) 696, 734, 736, 923, 1007
AclWI GGATC 2 cut(s) 47, 166
AcsI RAATTY 2 cut(s) 22, 448
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 4 cut(s) 137, 429, 465, 912
AfiI CCNNNNNNNGG 3 cut(s) 272, 344, 549
AgsI TTSAA 6 cut(s) 160, 223, 294, 397, 574, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
AluBI AGCT 3 cut(s) 299, 383, 793
AluI AGCT 3 cut(s) 299, 383, 793
Alw21I GWGCWC 1 cut(s) 795
Alw26I GTCTC 3 cut(s) 316, 506, 640
AlwI GGATC 2 cut(s) 47, 166
Ama87I CYCGRG 2 cut(s) 560, 775
AoxI GGCC 4 cut(s) 335, 399, 613, 1056
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 2 cut(s) 22, 448
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 2 cut(s) 251, 613
AsuHPI GGTGA 6 cut(s) 20, 252, 425, 760, 898, 911
AsuII TTCGAA 1 cut(s) 555
AvaI CYCGRG 2 cut(s) 560, 775
AvaII GGWCC 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 187
BanI GGYRCC 2 cut(s) 182, 367
BanII GRGCYC 2 cut(s) 742, 795
BauI CACGAG 2 cut(s) 788, 813
Bbv12I GWGCWC 1 cut(s) 795
BbvCI CCTCAGC 1 cut(s) 190
BbvI GCAGC 1 cut(s) 92
BccI CCATC 6 cut(s) 183, 224, 317, 615, 672, 779
BceAI ACGGC 1 cut(s) 600
BcoDI GTCTC 3 cut(s) 316, 506, 640
BfaI CTAG 2 cut(s) 881, 926
BfmI CTRYAG 1 cut(s) 684
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 251
BmeT110I CYCGRG 2 cut(s) 560, 775
BmgT120I GGNCC 2 cut(s) 251, 613
BmiI GGNNCC 6 cut(s) 184, 369, 614, 626, 700, 741
BmsI GCATC 2 cut(s) 62, 1000
BoxI GACNNNNGTC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 726
Bpu10I CCTNAGC 1 cut(s) 190
Bpu14I TTCGAA 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 76
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 3 cut(s) 496, 734, 825
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 344, 549
Bse118I RCCGGY 1 cut(s) 751
Bse1I ACTGG 2 cut(s) 743, 1006
Bse3DI GCAATG 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 496, 734, 825
BseGI GGATG 5 cut(s) 10, 454, 470, 664, 1015
BseLI CCNNNNNNNGG 3 cut(s) 272, 344, 549
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 2 cut(s) 204, 512
BseNI ACTGG 2 cut(s) 743, 1006
BseRI GAGGAG 3 cut(s) 113, 218, 221
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 1 cut(s) 92
Bsh1236I CGCG 2 cut(s) 736, 820
BshFI GGCC 4 cut(s) 337, 401, 615, 1058
BshNI GGYRCC 2 cut(s) 182, 367
BsiHKAI GWGCWC 1 cut(s) 795
BsiHKCI CYCGRG 2 cut(s) 560, 775
BsiSI CCGG 1 cut(s) 752
BslFI GGGAC 2 cut(s) 463, 807
BslI CCNNNNNNNGG 3 cut(s) 272, 344, 549
BsmAI GTCTC 3 cut(s) 316, 506, 640
BsmFI GGGAC 2 cut(s) 463, 807
BsnI GGCC 4 cut(s) 337, 401, 615, 1058
Bso31I GGTCTC 1 cut(s) 506
BsoBI CYCGRG 2 cut(s) 560, 775
Bsp119I TTCGAA 1 cut(s) 555
Bsp1286I GDGCHC 3 cut(s) 187, 742, 795
Bsp143I GATC 2 cut(s) 39, 171
BspACI CCGC 5 cut(s) 696, 734, 736, 923, 1007
BspANI GGCC 4 cut(s) 337, 401, 615, 1058
BspCNI CTCAG 2 cut(s) 203, 513
BspFNI CGCG 2 cut(s) 736, 820
BspLI GGNNCC 6 cut(s) 184, 369, 614, 626, 700, 741
BspPI GGATC 2 cut(s) 47, 166
BspQI GCTCTTC 2 cut(s) 306, 800
BspT104I TTCGAA 1 cut(s) 555
BspT107I GGYRCC 2 cut(s) 182, 367
BspTNI GGTCTC 1 cut(s) 506
BsrBI CCGCTC 1 cut(s) 696
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 751
BsrI ACTGG 2 cut(s) 743, 1006
BssAI RCCGGY 1 cut(s) 751
BssECI CCNNGG 3 cut(s) 496, 734, 825
BssMI GATC 2 cut(s) 39, 171
BssNAI GTATAC 1 cut(s) 661
BssSI CACGAG 2 cut(s) 788, 813
BssT1I CCWWGG 1 cut(s) 496
Bst1107I GTATAC 1 cut(s) 661
Bst2BI CACGAG 2 cut(s) 788, 813
Bst4CI ACNGT 2 cut(s) 347, 915
Bst6I CTCTTC 6 cut(s) 239, 306, 603, 800, 1020, 1082
BstBAI YACGTR 1 cut(s) 76
BstBI TTCGAA 1 cut(s) 555
BstC8I GCNNGC 1 cut(s) 738
BstDEI CTNAG 2 cut(s) 190, 521
BstDSI CCRYGG 2 cut(s) 734, 825
BstF5I GGATG 5 cut(s) 10, 454, 470, 664, 1015
BstFNI CGCG 2 cut(s) 736, 820
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 2 cut(s) 42, 174
BstMAI GTCTC 3 cut(s) 316, 506, 640
BstMBI GATC 2 cut(s) 39, 171
BstMWI GCNNNNNNNGC 3 cut(s) 191, 389, 398
BstPAI GACNNNNGTC 1 cut(s) 249
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 187
BstSNI TACGTA 1 cut(s) 76
BstUI CGCG 2 cut(s) 736, 820
BstV1I GCAGC 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstZ17I GTATAC 1 cut(s) 661
BsuRI GGCC 4 cut(s) 337, 401, 615, 1058
BtgI CCRYGG 2 cut(s) 734, 825
BtsCI GGATG 5 cut(s) 10, 454, 470, 664, 1015
Cac8I GCNNGC 1 cut(s) 738
CfoI GCGC 1 cut(s) 820
Cfr10I RCCGGY 1 cut(s) 751
Cfr13I GGNCC 2 cut(s) 251, 613
Cfr42I CCGCGG 1 cut(s) 737
CseI GACGC 1 cut(s) 809
Csp6I GTAC 4 cut(s) 136, 428, 464, 911
CviAII CATG 4 cut(s) 103, 148, 425, 544
CviQI GTAC 4 cut(s) 136, 428, 464, 911
DdeI CTNAG 2 cut(s) 190, 521
DpnI GATC 2 cut(s) 41, 173
DpnII GATC 2 cut(s) 39, 171
Eam1104I CTCTTC 6 cut(s) 239, 306, 603, 800, 1020, 1082
EarI CTCTTC 6 cut(s) 239, 306, 603, 800, 1020, 1082
Ecl136II GAGCTC 1 cut(s) 793
Eco105I TACGTA 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 496
Eco147I AGGCCT 2 cut(s) 337, 401
Eco24I GRGCYC 2 cut(s) 742, 795
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 1 cut(s) 251
Eco53kI GAGCTC 1 cut(s) 793
Eco57I CTGAAG 1 cut(s) 584
Eco88I CYCGRG 2 cut(s) 560, 775
EcoICRI GAGCTC 1 cut(s) 793
EcoT14I CCWWGG 1 cut(s) 496
EcoT38I GRGCYC 2 cut(s) 742, 795
ErhI CCWWGG 1 cut(s) 496
FaeI CATG 4 cut(s) 106, 151, 428, 547
FaqI GGGAC 2 cut(s) 463, 807
FatI CATG 4 cut(s) 102, 147, 424, 543
FauI CCCGC 2 cut(s) 729, 930
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 5 cut(s) 17, 441, 457, 651, 1022
FriOI GRGCYC 2 cut(s) 742, 795
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 2 cut(s) 881, 926
GlaI GCGC 1 cut(s) 819
GluI GCNGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 726
HaeIII GGCC 4 cut(s) 337, 401, 615, 1058
HapII CCGG 1 cut(s) 752
HgaI GACGC 1 cut(s) 809
HhaI GCGC 1 cut(s) 820
Hin1II CATG 4 cut(s) 106, 151, 428, 547
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HincII GTYRAC 1 cut(s) 942
HindII GTYRAC 1 cut(s) 942
HinfI GANTC 3 cut(s) 87, 245, 324
HpaII CCGG 1 cut(s) 752
HphI GGTGA 6 cut(s) 20, 252, 425, 760, 898, 911
Hpy166II GTNNAC 2 cut(s) 661, 942
Hpy188I TCNGA 2 cut(s) 522, 998
Hpy188III TCNNGA 2 cut(s) 503, 602
Hpy8I GTNNAC 2 cut(s) 661, 942
HpyAV CCTTC 5 cut(s) 205, 295, 391, 886, 1048
HpyCH4III ACNGT 2 cut(s) 347, 915
HpyCH4IV ACGT 3 cut(s) 75, 807, 944
HpyCH4V TGCA 4 cut(s) 53, 632, 787, 1013
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 389, 398
HpyF3I CTNAG 2 cut(s) 190, 521
HpySE526I ACGT 3 cut(s) 75, 807, 944
Hsp92II CATG 4 cut(s) 106, 151, 428, 547
HspAI GCGC 1 cut(s) 818
KspI CCGCGG 1 cut(s) 737
Kzo9I GATC 2 cut(s) 39, 171
LguI GCTCTTC 2 cut(s) 306, 800
LmnI GCTCC 3 cut(s) 156, 698, 745
LpnPI CCDG 7 cut(s) 234, 587, 672, 756, 765, 918, 987
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 2 cut(s) 62, 1000
MaeI CTAG 2 cut(s) 881, 926
MaeII ACGT 3 cut(s) 75, 807, 944
MaeIII GTNAC 2 cut(s) 347, 808
MalI GATC 2 cut(s) 41, 173
MbiI CCGCTC 1 cut(s) 696
MboI GATC 2 cut(s) 39, 171
MboII GAAGA 8 cut(s) 211, 226, 293, 296, 590, 787, 901, 1037
MfeI CAATTG 1 cut(s) 873
MhlI GDGCHC 3 cut(s) 187, 742, 795
MluCI AATT 5 cut(s) 22, 362, 420, 448, 873
MlyI GAGTC 2 cut(s) 239, 318
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 4 cut(s) 58, 180, 459, 1040
MspA1I CMGCKG 1 cut(s) 736
MspI CCGG 1 cut(s) 752
MunI CAATTG 1 cut(s) 873
MvnI CGCG 2 cut(s) 736, 820
MwoI GCNNNNNNNGC 3 cut(s) 191, 389, 398
NdeII GATC 2 cut(s) 39, 171
NlaIII CATG 4 cut(s) 106, 151, 428, 547
NlaIV GGNNCC 6 cut(s) 184, 369, 614, 626, 700, 741
NmuCI GTSAC 1 cut(s) 808
NspV TTCGAA 1 cut(s) 555
PaeR7I CTCGAG 1 cut(s) 560
PceI AGGCCT 2 cut(s) 337, 401
PciSI GCTCTTC 2 cut(s) 306, 800
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 2 cut(s) 239, 318
PpsI GAGTC 2 cut(s) 239, 318
Ppu21I YACGTR 1 cut(s) 76
PshAI GACNNNNGTC 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 795
PspN4I GGNNCC 6 cut(s) 184, 369, 614, 626, 700, 741
PspPI GGNCC 2 cut(s) 251, 613
PspXI VCTCGAGB 1 cut(s) 560
RsaI GTAC 4 cut(s) 137, 429, 465, 912
RsaNI GTAC 4 cut(s) 136, 428, 464, 911
RseI CAYNNNNRTG 4 cut(s) 58, 180, 459, 1040
SacI GAGCTC 1 cut(s) 795
SacII CCGCGG 1 cut(s) 737
SapI GCTCTTC 2 cut(s) 306, 800
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 2 cut(s) 39, 171
Sau96I GGNCC 2 cut(s) 251, 613
SchI GAGTC 2 cut(s) 239, 318
SduI GDGCHC 3 cut(s) 187, 742, 795
SfaNI GCATC 2 cut(s) 62, 1000
SfcI CTRYAG 1 cut(s) 684
Sfr274I CTCGAG 1 cut(s) 560
Sfr303I CCGCGG 1 cut(s) 737
SfuI TTCGAA 1 cut(s) 555
SgrBI CCGCGG 1 cut(s) 737
SinI GGWCC 1 cut(s) 251
SlaI CTCGAG 1 cut(s) 560
SmiMI CAYNNNNRTG 4 cut(s) 58, 180, 459, 1040
SmlI CTYRAG 2 cut(s) 537, 560
SmoI CTYRAG 2 cut(s) 537, 560
SnaBI TACGTA 1 cut(s) 76
Sse9I AATT 5 cut(s) 22, 362, 420, 448, 873
SseBI AGGCCT 2 cut(s) 337, 401
SsiI CCGC 5 cut(s) 696, 734, 736, 923, 1007
SspMI CTAG 2 cut(s) 881, 926
SstI GAGCTC 1 cut(s) 795
StuI AGGCCT 2 cut(s) 337, 401
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 2 cut(s) 347, 915
TaiI ACGT 3 cut(s) 78, 810, 947
TaqI TCGA 4 cut(s) 170, 504, 555, 561
TasI AATT 5 cut(s) 22, 362, 420, 448, 873
TatI WGTACW 1 cut(s) 463
TfiI GAWTC 1 cut(s) 87
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 4 cut(s) 16, 27, 277, 413
VpaK11BI GGWCC 1 cut(s) 251
XapI RAATTY 2 cut(s) 22, 448
XcmI CCANNNNNNNNNTGG 2 cut(s) 494, 1077
XhoI CTCGAG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 660
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 2 cut(s) 881, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.