MD10G1149100.v1.1

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0017243
Physical Location & Seq
Forward (+)
23631908 .. 23633307
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1149100.v1.1.491

Sequence Viewer

Length: 825 bp
ATGCCGGCTTCCATAAGCATGGATGAGAATATGGGGTTCCAGTACGGAGTTTCCAGGCCAGGGCTTAATCCACCAATTCAATTTGGGAATGGATTTTGTGGAGCAGGGTCAGGACTCATTCGAGGCGGATTGGGTGCGTATGGAAAGAAAATTTGTGGTTCAAGTTCTCAGTATGTTCAAAGCAATATAAGCAAATACTTCTCAGATCCTCAATACTACTTCCAAGTGAACTCCCACTACGTCCGAAACAAACTGAAGATAATTCTGTTTCCATTTCTGCACAGGGGGCACTGGACGAGGATGACAGAGCCAGTTGGAGGCAGGCTTTCGTATAACCCTCCAATTACTGACATACATGCCCCAGACCTGTACATTCCATTCATGACATTTGCAACGTACTTGGTTCTCGCTGGGATCTCACTAGGCCTTTCTGGGAAATTCAGCCCAGAGGCTATAAATTGGCAGTTTGTGAAGGGAATGGTGGGTTGGCTACTCCAAGTGATGTTGCTCAAGGCATCGTTGTCTTCGCTTGGGGGTGGAGAGGCACCTTTGTTAGACATGATAGCATATACTGGGTACACTTTCACAGGGTTGTGTGTGGCGGTTCTAGGGCGAATCACGCTAAGCTATGCTTACTACTTGATAATCATATGGACATCCATGTGCTCAGGCATCTTCTTGGCAAAGACAATTAAGATAACTTTGTATGCTGAGATCAACAGTTATGATGCAAACATGCATCATTATCTGTTGCTAGGTATCGCGTTTTCTCAGTTCCCGTTGATCATCTGGCTGAGCAACCCTACCGGGAATTGGTTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

275

Amino Acids

30.32

Weight (kDa)

9.06

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YIF1 PF03878 46 - 266 7.9e-53 YIF1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017243)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17570
malus_domestica MD10G1149100.v1.1
prunus_persica Prupe.8G187900_v2.0.a1
pyrus_communis pycom10g12870
rosa_chinensis RchiOBHm_Chr6g0282511
rosa_multiflora Rmu_sc0000283.1_g000013 Rmu_sc0005697.1_g000030
rosa_roxburghii Rroxscaffold_7G00186300
rosa_rugosa Rorug06G0147000
rosa_samantha Rh6AG260400 Rh6CG262600 Rh6DG254500
rosa_wichuraiana Rw6G022510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 544
AccII CGCG 1 cut(s) 764
AciI CCGC 2 cut(s) 126, 602
AclWI GGATC 2 cut(s) 200, 422
AcsI RAATTY 2 cut(s) 150, 437
AcuI CTGAAG 1 cut(s) 275
AfaI GTAC 4 cut(s) 44, 371, 398, 578
AfiI CCNNNNNNNGG 3 cut(s) 60, 317, 813
AgsI TTSAA 3 cut(s) 80, 162, 179
AjnI CCWGG 2 cut(s) 53, 58
AjuI GAANNNNNNNTTGG 2 cut(s) 469, 501
AluBI AGCT 1 cut(s) 627
AluI AGCT 1 cut(s) 627
Alw21I GWGCWC 1 cut(s) 668
AlwI GGATC 2 cut(s) 200, 422
AoxI GGCC 2 cut(s) 56, 424
ApoI RAATTY 2 cut(s) 150, 437
AsuC2I CCSGG 1 cut(s) 808
BaeGI GKGCMC 1 cut(s) 291
BanI GGYRCC 1 cut(s) 544
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 668
BciT130I CCWGG 2 cut(s) 55, 60
BclI TGATCA 1 cut(s) 783
BcnI CCSGG 1 cut(s) 808
BfaI CTAG 3 cut(s) 422, 608, 755
BlpI GCTNAGC 2 cut(s) 623, 794
Bme1390I CCNGG 3 cut(s) 55, 60, 808
BmiI GGNNCC 2 cut(s) 38, 546
BmrFI CCNGG 3 cut(s) 55, 60, 808
BmrI ACTGGG 1 cut(s) 582
BmsI GCATC 4 cut(s) 524, 681, 718, 748
BmuI ACTGGG 1 cut(s) 582
BpiI GAAGAC 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 667
Bpu1102I GCTNAGC 2 cut(s) 623, 794
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 808
BsaJI CCNNGG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 317, 813
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 4 cut(s) 40, 296, 311, 577
BseBI CCWGG 2 cut(s) 55, 60
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 3 cut(s) 28, 306, 656
BseLI CCNNNNNNNGG 3 cut(s) 60, 317, 813
BseMII CTCAG 6 cut(s) 182, 216, 681, 702, 785, 785
BseNI ACTGG 4 cut(s) 40, 296, 311, 577
BseSI GKGCMC 1 cut(s) 291
BseYI CCCAGC 1 cut(s) 410
BsgI GTGCAG 1 cut(s) 263
Bsh1236I CGCG 1 cut(s) 764
BshFI GGCC 2 cut(s) 58, 426
BshNI GGYRCC 1 cut(s) 544
BsiHKAI GWGCWC 1 cut(s) 668
BsiSI CCGG 2 cut(s) 5, 807
BslI CCNNNNNNNGG 3 cut(s) 60, 317, 813
BsnI GGCC 2 cut(s) 58, 426
Bsp1286I GDGCHC 2 cut(s) 291, 668
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 4 cut(s) 205, 414, 714, 783
Bsp1720I GCTNAGC 2 cut(s) 623, 794
BspACI CCGC 2 cut(s) 126, 602
BspANI GGCC 2 cut(s) 58, 426
BspCNI CTCAG 6 cut(s) 181, 215, 680, 703, 784, 786
BspFNI CGCG 1 cut(s) 764
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 38, 546
BspPI GGATC 2 cut(s) 200, 422
BspT107I GGYRCC 1 cut(s) 544
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 4 cut(s) 40, 296, 311, 577
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 4 cut(s) 205, 414, 714, 783
Bst2UI CCWGG 2 cut(s) 55, 60
Bst4CI ACNGT 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 6, 323
BstDEI CTNAG 7 cut(s) 168, 202, 623, 667, 711, 771, 794
BstF5I GGATG 3 cut(s) 28, 306, 656
BstFNI CGCG 1 cut(s) 764
BstKTI GATC 4 cut(s) 208, 417, 717, 786
BstMBI GATC 4 cut(s) 205, 414, 714, 783
BstMWI GCNNNNNNNGC 3 cut(s) 189, 286, 619
BstNI CCWGG 2 cut(s) 55, 60
BstNSI RCATGY 2 cut(s) 359, 739
BstSCI CCNGG 3 cut(s) 53, 58, 806
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 1 cut(s) 764
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 2 cut(s) 205, 414
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 2 cut(s) 205, 414
BsuRI GGCC 2 cut(s) 58, 426
BtsCI GGATG 3 cut(s) 28, 306, 656
BtsIMutI CAGTG 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 6, 323
CciI TCATGA 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 4
Csp6I GTAC 4 cut(s) 43, 370, 397, 577
CviAII CATG 6 cut(s) 19, 356, 382, 559, 661, 736
CviQI GTAC 4 cut(s) 43, 370, 397, 577
DdeI CTNAG 7 cut(s) 168, 202, 623, 667, 711, 771, 794
DpnI GATC 4 cut(s) 207, 416, 716, 785
DpnII GATC 4 cut(s) 205, 414, 714, 783
EciI GGCGGA 1 cut(s) 141
Eco147I AGGCCT 1 cut(s) 426
Eco57I CTGAAG 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 53, 58
EcoT22I ATGCAT 1 cut(s) 741
FaeI CATG 6 cut(s) 22, 359, 385, 562, 664, 739
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FatI CATG 6 cut(s) 18, 355, 381, 558, 660, 735
FauNDI CATATG 1 cut(s) 650
FbaI TGATCA 1 cut(s) 783
FokI GGATG 3 cut(s) 35, 313, 643
FspBI CTAG 3 cut(s) 422, 608, 755
GsaI CCCAGC 1 cut(s) 414
HaeIII GGCC 2 cut(s) 58, 426
HapII CCGG 2 cut(s) 5, 807
Hin1II CATG 6 cut(s) 22, 359, 385, 562, 664, 739
HinfI GANTC 2 cut(s) 114, 615
HpaII CCGG 2 cut(s) 5, 807
Hpy166II GTNNAC 2 cut(s) 229, 579
Hpy188I TCNGA 2 cut(s) 205, 245
Hpy188III TCNNGA 3 cut(s) 111, 382, 822
Hpy8I GTNNAC 2 cut(s) 229, 579
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 1 cut(s) 722
HpyCH4IV ACGT 2 cut(s) 240, 395
HpyCH4V TGCA 4 cut(s) 280, 392, 731, 739
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 286, 619
HpyF3I CTNAG 7 cut(s) 168, 202, 623, 667, 711, 771, 794
HpySE526I ACGT 2 cut(s) 240, 395
Hsp92II CATG 6 cut(s) 22, 359, 385, 562, 664, 739
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 783
Kzo9I GATC 4 cut(s) 205, 414, 714, 783
LmnI GCTCC 1 cut(s) 101
LweI GCATC 4 cut(s) 524, 681, 718, 748
MaeI CTAG 3 cut(s) 422, 608, 755
MaeII ACGT 2 cut(s) 240, 395
MalI GATC 4 cut(s) 207, 416, 716, 785
MboI GATC 4 cut(s) 205, 414, 714, 783
MboII GAAGA 3 cut(s) 268, 516, 667
MflI RGATCY 2 cut(s) 205, 414
MhlI GDGCHC 2 cut(s) 291, 668
MluCI AATT 9 cut(s) 75, 80, 150, 261, 342, 437, 457, 690, 811
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 7 cut(s) 116, 219, 291, 311, 348, 442, 535
Mph1103I ATGCAT 1 cut(s) 741
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 2 cut(s) 66, 693
MslI CAYNNNNRTG 2 cut(s) 17, 661
MspI CCGG 2 cut(s) 5, 807
MspR9I CCNGG 3 cut(s) 55, 60, 808
MvaI CCWGG 2 cut(s) 55, 60
MvnI CGCG 1 cut(s) 764
MwoI GCNNNNNNNGC 3 cut(s) 189, 286, 619
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 808
NdeI CATATG 1 cut(s) 650
NdeII GATC 4 cut(s) 205, 414, 714, 783
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 6 cut(s) 22, 359, 385, 562, 664, 739
NlaIV GGNNCC 2 cut(s) 38, 546
NsiI ATGCAT 1 cut(s) 741
NspI RCATGY 2 cut(s) 359, 739
PagI TCATGA 1 cut(s) 381
PceI AGGCCT 1 cut(s) 426
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 1 cut(s) 615
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 53, 58
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 2 cut(s) 53, 58
PspN4I GGNNCC 2 cut(s) 38, 546
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
PsuI RGATCY 2 cut(s) 205, 414
RsaI GTAC 4 cut(s) 44, 371, 398, 578
RsaNI GTAC 4 cut(s) 43, 370, 397, 577
RseI CAYNNNNRTG 2 cut(s) 17, 661
SaqAI TTAA 2 cut(s) 66, 693
Sau3AI GATC 4 cut(s) 205, 414, 714, 783
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 3 cut(s) 55, 60, 808
SduI GDGCHC 2 cut(s) 291, 668
SetI ASST 6 cut(s) 243, 369, 398, 550, 629, 760
SfaNI GCATC 4 cut(s) 524, 681, 718, 748
SmiMI CAYNNNNRTG 2 cut(s) 17, 661
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 9 cut(s) 75, 80, 150, 261, 342, 437, 457, 690, 811
SseBI AGGCCT 1 cut(s) 426
SsiI CCGC 2 cut(s) 126, 602
SspMI CTAG 3 cut(s) 422, 608, 755
StuI AGGCCT 1 cut(s) 426
StyD4I CCNGG 3 cut(s) 53, 58, 806
TaaI ACNGT 1 cut(s) 722
TaiI ACGT 2 cut(s) 243, 398
TaqI TCGA 1 cut(s) 121
TasI AATT 9 cut(s) 75, 80, 150, 261, 342, 437, 457, 690, 811
TatI WGTACW 1 cut(s) 369
TfiI GAWTC 1 cut(s) 615
Tru1I TTAA 2 cut(s) 66, 693
Tru9I TTAA 2 cut(s) 66, 693
TscAI CASTG 1 cut(s) 296
TspDTI ATGAA 1 cut(s) 370
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 1 cut(s) 296
XapI RAATTY 2 cut(s) 150, 437
XceI RCATGY 2 cut(s) 359, 739
XspI CTAG 3 cut(s) 422, 608, 755
Zsp2I ATGCAT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.