Rorug06G0147000

YIF1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017243
Physical Location & Seq
Reverse (-)
20982775 .. 20988158
5384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0147000.1

Sequence Viewer

Length: 3945 bp
ATGGCCATCCAATTGAGAGCTTCTTCTTCTCTCTCCCCTGCTTCTTCCACCGGTTCGTGGAGACATGATGTGTTTCTGAGTTTCAGAGGCGAGGATACGCGCTACAATTTTACAGATCATTTGCACAGAAATTTGGTTCAAAGGGGGATCAACACTTTCATAGATGATGATCTTACAAGAGGAGAGGAAATATCAGAAGCACTTCTCCGAGCCATTGAAGGATCACAGTTCTCTCTCATTGTGTTCTCTGAAAACTATGCATCCTCAAAGTGGTGTTTGGATGAACTGGTTCATATCCTTGGATGTAGAAAATCAAAGAAACAAATGGTGTGGCCAATCTTTTACAAAGTGGATCCATCGGACGTAAGACACCAAAGAGGCAAATTTGGTGAGGCAGTTGCAGAGCATGAACTCAGATTCAAGGATGACATGGACAAGGTGTCAAGATGGAGAGCAGCTCTTTTAGAAGCTGCTAATTTGTCTGGGTGGCATTTCTCGAGCGGGCATGAAGCTAAATTTATTGAGGAAATTGTTGAAAAGGTCTCTACACAACTAAAAGATCGTATCAATTTGGATGTGGCAGAGTGTCCGGTTGGGATAGAATCTCGCCTACAAGACATGCTTGAAATTTTAGATGTTAAGGGAGGTGATCATATACACATGGTAGGGATATGGGGAAGTGGTGGAATAGGGAAGACAACAATTGCGAAAGCTGTTTACAATACAGTTGCCCATGAGTTTGATGGTAGCTGCTTTTTGCCAAATGTTAGAGAAGGTTCAGAGCAACATGGAGGTTTAGTCGACCTACAAAACACCATTCTTTCAGAGATTCTAGGGGCCAATGAACTGAGAGTAATCAATGTTGATAAAGGAATTACTTTGTTGCGGGAGAGGTTGAGATGTAAAAGGATTCTTTTAGTTCTTGATGATGTGGGTCAATTGGACCAGTTAAAGAAATTAGCCGGAGCAACTGATTGGTTTGGTCCTGGCAGTAGAGTTATAATAACAACTAGAGATAAGCAATTATTGATTGCTCATCGAGTGAAAAAAAATAAAATATACGAGGCCCGGAGACTAGATCATGATGAAGCTTGTGAGCTCTTCTGTGCAAATGCCTTGATGACTAATATGGATAATGATGAAAAACACGCAGTTAACACTATTGTTAACTATGCTGAAGGCCTTCCATTAGCGCTGAAAGTTTTAGGTTCACATCTATGTAGTAGATCTATAGATCAGTGGCAAGCTTTATTAGATGGTCATAGAAGAAGTCCTCTCCAAGGCATTCAAAAGATTCTCAAAATCAGTTACGATGCACTGGAACAACAAGAGAGAGAAATTTTCCTTGACATTGCGTGCTTCTTCAAAAATAAAAATAAGAACGATGTGATAGCAATATTAGAAGGTTGTGATTACAACAACCCTATGTCTAGTATTGAAGTTCTCGAAGAAAAGGCCCTCCTAAATGTTGGACATGCTGATATTTTCATGCATGATTTGCTAGAAGAAATGGGGAAAAAAATAGTTCATGAAGAGTCGCCTAAAGAGCCTGGTCGTCGCACCAGATTGTGGTATCACAAAGATGTTCTCCAAGTTCTAACAGAGAACACAGGAACAAGTAACATTGAAGGCATAATGGTAAAGATGCCAACAGCAGATGAGATACACTTGAGTCCTAAATGCTTCAAAAAGATGAGAAATCTTAAAATTTTTATAAACGTCAATGGACGGTTTTGTGGAAAGGTTGATTATTTTCCAAATCAATTGCGGTTACTTGATTGGCGTGATTGTCCGCTACAATCTTTGCCCTCCGATTTTAATACGAAGAATCTGGTTCAACTTAGTATGCCTTGCAGCCGCATCTCACGTTTTGGAGAAGGATTCAAGAGTATGGAAAATCTGAAATCCTTAAATTTGAAGAAATGTGAATTCCTAGTACAAAGCCCAGACCTGTCTGGAAGCCCAAACTTAGAGTTTCTGGATCTAAGTTTCTGTAAAAAGTTAAAGAAGATTGACCCTTCGGTTGGATCCCTCAAAAAGCTTGTTCGACTGGATCTAGGTTCCTGTACAAGTTTAAAGAAGATTCACCATTCGGTTGGATCCCTCAAAAAGCTTGTTCACCTGAATCTACATTGGTGTAAAAGGTTAGAGGAGGTTCACCCTTCGGTTGGATCCCTCGAAAAGCTTGTTCACCTGGATCTTGAAGGATGCTCTAAACTTCGGATGCTACCTAGCGAAGTCAACTGGAGATCCCTCCAAACCATTGTTCTTCACTGGTGCAGCAAGCTGGAGAGTTTTCCTGAAATTGTGGGAGAGATGAAATACATGACATCCTTGAGTCTAACCGGCACTGCCATCAAAGCATTGCCTTCATCCTTGAGTCTAATTAACCTTGAATCGTTGGAGTTATGTGATTGTGGAAACCTCACAGATCTACCTTATGAATTGCAAAATCTGAAATCCTTAAAATTGAAGGGATGTGGATTCCTACGAAGCCCAGACCTGTCTGGATGCCCAAACTTAGAGTTCCTGGATCTAAGTGGGTGTAAAAGGTTAGAGGAGGTTCACCCTTCGGTTGGATCCCTCGAAAAGCTTGTTGAACTGGATCTTGAAGGATGCTCTAACCTCGTGACGCTTCCTGAAAAAGTTAACTGGAGATCCCTCCAAACCATTAATCTTGACTATTGCAGAATGCTGGAGAGTTTTCCTGAAATTGTGGGAGAGATGAAATACATGACATCCTTGACTTTATTGGCCGTTGGCATCAAAGCATTGCCTTCATCCATTAGATATCTAATTAACCTTGAACGGTTGGTGTTAGATAAGTGTGGAAACCTCACAGATCTACCTTGCAGCATCTATGAATTGCAAAATCTGGAGGTGGTTCGTTTGATGAAGTGCCCAAAACTCGTAAGATTCCCCGAATGCAGCAACGAAATTGACGACTTGTGGTCACTCAGTTTGAGCGGCTGCACGAGGCTCGTAGAAATTCCAGAGCTTCCACCAAGTATTCGCCGGTTGGATGTGAGTGGTTGCGTATCATTGGAAAGAATTTCGAAGTTGTCAAACATTTTGGAGCGTAAAGAATCACAAATGATTGAGGAGATGGACTTGACTAATTGCTGGAGACTCTGTGAAAATTTGGTTCAGATGGCAAACAAGGATGATGATGATGATGATGATGAGGTGGGCGCTGATCTCTTCTCTCGACTCCTATCTTCTCAGCAATCTAAATTCACAATTAAATTTCCAGTTCCAAGAAGCGAGGTTCCACAGTGGTTCAGCTGTCAAATGGATTTCATGGGACATCAACGGTTTGAATTTTGTATTGAAACACTTGCAAATTTCAAATGGGACAACACAGGATTGGCTCTCTGTGTTGCTCTTGATCAAAACCTGGTAGGTACTCCTTTTGTGGTCCATATTCACATCAATGAAGTGAGTGTTTCAAATCTCTCCCACTACTCTCTGTGTGGTGATGAGAGACATTTGGTGGAACAGTCGGATCATGTGTGGCTGCACTATGTTCCCTTCCTTGAAATATGGCGATTTGGTTATATGCGTCCATTGCCCCCTTTTACGTGTCGAGTCATTATTTATGAATATTACGCCGAGTCCTCCATAAAAAGCTGTGGTGTCCACCTTGTAATGCCACCCAATGAAGACGTTTGTATGAAGCTAATCCGTGCAGAGAACCTCACCAGTGAACTTTCTGATGATGAATTTTCTGATGATGATGAAGTTGATAAGGACTTCTGGGACAATTATCGTCCCAAACAGAGGACTGACTGCAAAGAATTATATACTGATGCACAACTGGAAAACGAAAAGCTGGACTGCCCTGAGGAATCTAAGGAAGCCGTTGCATCACTAATGGTTACTGCAGCTAGATTTTCTGATTTACCAGAGTTTTGTGACATTAGGCCACTATTTCAAGAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YIF1

Protein Analysis

1314

Amino Acids

149.61

Weight (kDa)

5.6

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 187 1.5e-55 TIR domain
TIR_2 PF13676 24 - 119 3.4e-15 TIR domain
NB-ARC PF00931 216 - 376 1.6e-20 NB-ARC domain
WHD_ROQ1 PF23282 441 - 513 7.5e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 751 - 832 6.8e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 857 - 967 5.1e-06 Leucine-rich repeat region
LRR_13 PF23286 887 - 970 1.7e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017243)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17570
malus_domestica MD10G1149100.v1.1
prunus_persica Prupe.8G187900_v2.0.a1
pyrus_communis pycom10g12870
rosa_chinensis RchiOBHm_Chr6g0282511
rosa_multiflora Rmu_sc0000283.1_g000013 Rmu_sc0005697.1_g000030
rosa_roxburghii Rroxscaffold_7G00186300
rosa_rugosa Rorug06G0147000
rosa_samantha Rh6AG260400 Rh6CG262600 Rh6DG254500
rosa_wichuraiana Rw6G022510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1001, 1715
AccB7I CCANNNNNTGG 1 cut(s) 1569
AccBSI CCGCTC 2 cut(s) 501, 2967
AccI GTMKAC 1 cut(s) 801
AccII CGCG 1 cut(s) 100
AciI CCGC 6 cut(s) 501, 886, 1768, 1793, 1858, 2967
AcoI YGGCCR 3 cut(s) 3, 332, 2754
AcuI CTGAAG 1 cut(s) 1197
AfaI GTAC 3 cut(s) 1938, 2068, 3406
AfeI AGCGCT 1 cut(s) 1194
AfiI CCNNNNNNNGG 8 cut(s) 57, 270, 1280, 1569, 2024, 2168, 2576, 3780
AflIII ACRYGT 1 cut(s) 3581
AgeI ACCGGT 1 cut(s) 50
AhdI GACNNNNNGTC 2 cut(s) 439, 2950
AjnI CCWGG 5 cut(s) 985, 1549, 2193, 2529, 3396
AjuI GAANNNNNNNTTGG 2 cut(s) 2250, 2282
Alw21I GWGCWC 1 cut(s) 1101
Alw26I GTCTC 5 cut(s) 55, 547, 1066, 3121, 3478
Ama87I CYCGRG 1 cut(s) 496
Aor51HI AGCGCT 1 cut(s) 1194
AoxI GGCC 8 cut(s) 3, 332, 837, 1065, 1180, 1455, 2754, 3923
AseI ATTAAT 1 cut(s) 2673
AsiGI ACCGGT 1 cut(s) 50
Asp700I GAANNNNTTC 3 cut(s) 201, 288, 1182
AspLEI GCGC 3 cut(s) 102, 1195, 3194
AspS9I GGNCC 6 cut(s) 837, 943, 983, 1066, 1456, 3418
AsuC2I CCSGG 1 cut(s) 1069
AsuHPI GGTGA 9 cut(s) 401, 659, 2078, 2111, 2150, 2183, 2558, 3488, 3691
AsuII TTCGAA 1 cut(s) 3056
AvaI CYCGRG 1 cut(s) 496
AvaII GGWCC 3 cut(s) 943, 983, 3418
AxyI CCTNAGG 1 cut(s) 3843
BaeGI GKGCMC 1 cut(s) 2903
BalI TGGCCA 2 cut(s) 5, 334
BamHI GGATCC 5 cut(s) 352, 2027, 2099, 2171, 2579
BanII GRGCYC 1 cut(s) 1101
BauI CACGAG 2 cut(s) 2627, 2974
BbsI GAAGAC 2 cut(s) 701, 3669
Bbv12I GWGCWC 1 cut(s) 1101
BccI CCATC 8 cut(s) 14, 364, 441, 737, 1250, 2363, 3100, 3145
BceAI ACGGC 2 cut(s) 2741, 3845
BcgI CGANNNNNNTGC 2 cut(s) 2962, 2996
BciT130I CCWGG 5 cut(s) 987, 1551, 2195, 2531, 3398
BciVI GTATCC 1 cut(s) 88
BclI TGATCA 2 cut(s) 649, 3388
BcnI CCSGG 1 cut(s) 1069
BcoDI GTCTC 5 cut(s) 55, 547, 1066, 3121, 3478
BfaI CTAG 9 cut(s) 833, 1011, 1076, 1431, 1502, 1934, 2057, 2232, 3888
BfmI CTRYAG 2 cut(s) 1230, 3882
BfoI RGCGCY 2 cut(s) 1196, 3195
BfuI GTATCC 1 cut(s) 88
BglII AGATCT 3 cut(s) 1226, 2431, 2842
Bme1390I CCNGG 6 cut(s) 987, 1069, 1551, 2195, 2531, 3398
Bme18I GGWCC 3 cut(s) 943, 983, 3418
BmeRI GACNNNNNGTC 2 cut(s) 439, 2950
BmeT110I CYCGRG 1 cut(s) 496
BmgT120I GGNCC 6 cut(s) 837, 943, 983, 1066, 1456, 3418
BmiI GGNNCC 8 cut(s) 354, 838, 2029, 2062, 2101, 2173, 2581, 3270
BmrFI CCNGG 6 cut(s) 987, 1069, 1551, 2195, 2531, 3398
BpiI GAAGAC 2 cut(s) 701, 3669
BplI GAGNNNNNCTC 2 cut(s) 442, 474
BpmI CTGGAG 6 cut(s) 2266, 2309, 2674, 2717, 2897, 3145
Bpu14I TTCGAA 1 cut(s) 3056
BpuEI CTTGAG 3 cut(s) 1690, 2356, 2398
BpuMI CCSGG 1 cut(s) 1069
BsaAI YACGTR 1 cut(s) 3582
BsaBI GATNNNNATC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 547
BsaJI CCNNGG 2 cut(s) 298, 1279
BsaWI WCCGGW 2 cut(s) 50, 589
BsaXI ACNNNNNCTCC 4 cut(s) 783, 813, 3118, 3148
Bsc4I CCNNNNNNNGG 8 cut(s) 57, 270, 1280, 1569, 2024, 2168, 2576, 3780
Bse118I RCCGGY 3 cut(s) 50, 2345, 3015
Bse21I CCTNAGG 1 cut(s) 3843
Bse3DI GCAATG 4 cut(s) 1350, 2363, 2771, 3566
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 5 cut(s) 987, 1551, 2195, 2531, 3398
BseDI CCNNGG 2 cut(s) 298, 1279
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 8 cut(s) 57, 270, 1280, 1569, 2024, 2168, 2576, 3780
BseMI GCAATG 4 cut(s) 1350, 2363, 2771, 3566
BseMII CTCAG 6 cut(s) 68, 427, 839, 2971, 3236, 3834
BseRI GAGGAG 4 cut(s) 195, 2165, 2573, 3116
BseSI GKGCMC 1 cut(s) 2903
BsgI GTGCAG 4 cut(s) 2299, 2956, 3503, 3708
Bsh1236I CGCG 1 cut(s) 100
BshFI GGCC 8 cut(s) 5, 334, 839, 1067, 1182, 1457, 2756, 3925
BshTI ACCGGT 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 1101
BsiHKCI CYCGRG 1 cut(s) 496
BsiSI CCGG 6 cut(s) 51, 590, 963, 1069, 2346, 3016
BslFI GGGAC 4 cut(s) 3318, 3368, 3756, 3773
BslI CCNNNNNNNGG 8 cut(s) 57, 270, 1280, 1569, 2024, 2168, 2576, 3780
BsmAI GTCTC 5 cut(s) 55, 547, 1066, 3121, 3478
BsmFI GGGAC 4 cut(s) 3318, 3368, 3756, 3773
BsmI GAATGC 3 cut(s) 1284, 2697, 2930
BsnI GGCC 8 cut(s) 5, 334, 839, 1067, 1182, 1457, 2756, 3925
Bso31I GGTCTC 1 cut(s) 547
BsoBI CYCGRG 1 cut(s) 496
Bsp119I TTCGAA 1 cut(s) 3056
Bsp1286I GDGCHC 2 cut(s) 1101, 2903
Bsp1407I TGTACA 1 cut(s) 2066
BspACI CCGC 6 cut(s) 501, 886, 1768, 1793, 1858, 2967
BspANI GGCC 8 cut(s) 5, 334, 839, 1067, 1182, 1457, 2756, 3925
BspCNI CTCAG 6 cut(s) 69, 426, 840, 2970, 3235, 3835
BspFNI CGCG 1 cut(s) 100
BspHI TCATGA 2 cut(s) 1081, 1528
BspLI GGNNCC 8 cut(s) 354, 838, 2029, 2062, 2101, 2173, 2581, 3270
BspMAI CTGCAG 1 cut(s) 3886
BspQI GCTCTTC 1 cut(s) 1106
BspT104I TTCGAA 1 cut(s) 3056
BspTNI GGTCTC 1 cut(s) 547
BsrBI CCGCTC 2 cut(s) 501, 2967
BsrDI GCAATG 4 cut(s) 1350, 2363, 2771, 3566
BsrFI RCCGGY 3 cut(s) 50, 2345, 3015
BsrGI TGTACA 1 cut(s) 2066
BssAI RCCGGY 3 cut(s) 50, 2345, 3015
BssECI CCNNGG 2 cut(s) 298, 1279
BssSI CACGAG 2 cut(s) 2627, 2974
BssT1I CCWWGG 2 cut(s) 298, 1279
Bst2BI CACGAG 2 cut(s) 2627, 2974
Bst2UI CCWGG 5 cut(s) 987, 1551, 2195, 2531, 3398
Bst4CI ACNGT 7 cut(s) 228, 727, 1731, 2811, 3276, 3315, 3501
Bst6I CTCTTC 3 cut(s) 1106, 1527, 3206
BstAPI GCANNNNNTGC 1 cut(s) 1498
BstAUI TGTACA 1 cut(s) 2066
BstBAI YACGTR 1 cut(s) 3582
BstBI TTCGAA 1 cut(s) 3056
BstC8I GCNNGC 4 cut(s) 503, 1245, 1357, 2285
BstENI CCTNNNNNAGG 1 cut(s) 1278
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 2 cut(s) 1196, 3195
BstHHI GCGC 3 cut(s) 102, 1195, 3194
BstMAI GTCTC 5 cut(s) 55, 547, 1066, 3121, 3478
BstMWI GCNNNNNNNGC 4 cut(s) 1498, 1546, 2360, 3568
BstNI CCWGG 5 cut(s) 987, 1551, 2195, 2531, 3398
BstNSI RCATGY 2 cut(s) 622, 1478
BstSCI CCNGG 6 cut(s) 985, 1067, 1549, 2193, 2529, 3396
BstSFI CTRYAG 2 cut(s) 1230, 3882
BstSLI GKGCMC 1 cut(s) 2903
BstUI CGCG 1 cut(s) 100
BstV2I GAAGAC 2 cut(s) 701, 3669
BstXI CCANNNNNNTGG 1 cut(s) 2096
Bsu36I CCTNAGG 1 cut(s) 3843
BsuI GTATCC 1 cut(s) 88
BsuRI GGCC 8 cut(s) 5, 334, 839, 1067, 1182, 1457, 2756, 3925
BtsI GCAGTG 1 cut(s) 2349
BtsIMutI CAGTG 6 cut(s) 1244, 1316, 2272, 2349, 3281, 3709
Cac8I GCNNGC 4 cut(s) 503, 1245, 1357, 2285
CciI TCATGA 2 cut(s) 1081, 1528
CfoI GCGC 3 cut(s) 102, 1195, 3194
Cfr10I RCCGGY 3 cut(s) 50, 2345, 3015
Cfr13I GGNCC 6 cut(s) 837, 943, 983, 1066, 1456, 3418
CseI GACGC 2 cut(s) 2641, 3551
CsiI ACCWGGT 1 cut(s) 3396
Csp6I GTAC 3 cut(s) 1937, 2067, 3405
CspAI ACCGGT 1 cut(s) 50
CspCI CAANNNNNGTGG 2 cut(s) 311, 346
CviQI GTAC 3 cut(s) 1937, 2067, 3405
DraI TTTAAA 1 cut(s) 2076
DriI GACNNNNNGTC 2 cut(s) 439, 2950
EaeI YGGCCR 3 cut(s) 3, 332, 2754
Eam1104I CTCTTC 3 cut(s) 1106, 1527, 3206
Eam1105I GACNNNNNGTC 2 cut(s) 439, 2950
EarI CTCTTC 3 cut(s) 1106, 1527, 3206
Ecl136II GAGCTC 1 cut(s) 1099
Eco130I CCWWGG 2 cut(s) 298, 1279
Eco147I AGGCCT 1 cut(s) 1182
Eco24I GRGCYC 1 cut(s) 1101
Eco31I GGTCTC 1 cut(s) 547
Eco32I GATATC 1 cut(s) 2792
Eco47I GGWCC 3 cut(s) 943, 983, 3418
Eco47III AGCGCT 1 cut(s) 1194
Eco53kI GAGCTC 1 cut(s) 1099
Eco57I CTGAAG 1 cut(s) 1197
Eco81I CCTNAGG 1 cut(s) 3843
Eco88I CYCGRG 1 cut(s) 496
EcoICRI GAGCTC 1 cut(s) 1099
EcoNI CCTNNNNNAGG 1 cut(s) 1278
EcoO109I RGGNCCY 1 cut(s) 1456
EcoRI GAATTC 1 cut(s) 1928
EcoRII CCWGG 5 cut(s) 985, 1549, 2193, 2529, 3396
EcoRV GATATC 1 cut(s) 2792
EcoT14I CCWWGG 2 cut(s) 298, 1279
EcoT22I ATGCAT 2 cut(s) 262, 1494
EcoT38I GRGCYC 1 cut(s) 1101
ErhI CCWWGG 2 cut(s) 298, 1279
FalI AAGNNNNNCTT 2 cut(s) 606, 638
FaqI GGGAC 4 cut(s) 3318, 3368, 3756, 3773
FauI CCCGC 2 cut(s) 494, 879
FbaI TGATCA 2 cut(s) 649, 3388
FblI GTMKAC 1 cut(s) 801
FriOI GRGCYC 1 cut(s) 1101
FspBI CTAG 9 cut(s) 833, 1011, 1076, 1431, 1502, 1934, 2057, 2232, 3888
GlaI GCGC 3 cut(s) 101, 1194, 3193
GsuI CTGGAG 6 cut(s) 2266, 2309, 2674, 2717, 2897, 3145
HaeII RGCGCY 2 cut(s) 1196, 3195
HaeIII GGCC 8 cut(s) 5, 334, 839, 1067, 1182, 1457, 2756, 3925
HapII CCGG 6 cut(s) 51, 590, 963, 1069, 2346, 3016
HgaI GACGC 2 cut(s) 2641, 3551
HhaI GCGC 3 cut(s) 102, 1195, 3194
Hin6I GCGC 3 cut(s) 100, 1193, 3192
HinP1I GCGC 3 cut(s) 100, 1193, 3192
HincII GTYRAC 5 cut(s) 802, 1156, 1168, 2242, 2650
HindII GTYRAC 5 cut(s) 802, 1156, 1168, 2242, 2650
HindIII AAGCTT 6 cut(s) 1089, 1245, 2039, 2111, 2183, 2591
HpaI GTTAAC 3 cut(s) 1156, 1168, 2650
HpaII CCGG 6 cut(s) 51, 590, 963, 1069, 2346, 3016
HphI GGTGA 9 cut(s) 401, 659, 2078, 2111, 2150, 2183, 2558, 3488, 3691
Hpy99I CGWCG 1 cut(s) 1560
HpyCH4III ACNGT 7 cut(s) 228, 727, 1731, 2811, 3276, 3315, 3501
HpyCH4IV ACGT 5 cut(s) 363, 1719, 1867, 3581, 3666
HpyF10VI GCNNNNNNNGC 4 cut(s) 1498, 1546, 2360, 3568
HpySE526I ACGT 5 cut(s) 363, 1719, 1867, 3581, 3666
HspAI GCGC 3 cut(s) 100, 1193, 3192
Ksp22I TGATCA 2 cut(s) 649, 3388
KspAI GTTAAC 3 cut(s) 1156, 1168, 2650
LguI GCTCTTC 1 cut(s) 1106
LmnI GCTCC 2 cut(s) 965, 3076
MabI ACCWGGT 1 cut(s) 3396
MaeI CTAG 9 cut(s) 833, 1011, 1076, 1431, 1502, 1934, 2057, 2232, 3888
MaeII ACGT 5 cut(s) 363, 1719, 1867, 3581, 3666
MaeIII GTNAC 7 cut(s) 1307, 1619, 1770, 2629, 2952, 3877, 3914
MbiI CCGCTC 2 cut(s) 501, 2967
MfeI CAATTG 4 cut(s) 11, 702, 938, 1763
MhlI GDGCHC 2 cut(s) 1101, 2903
MlsI TGGCCA 2 cut(s) 5, 334
MluNI TGGCCA 2 cut(s) 5, 334
MlyI GAGTC 8 cut(s) 1544, 1681, 2347, 2389, 3123, 3204, 3597, 3623
MmeI TCCRAC 8 cut(s) 1450, 2005, 2077, 2149, 2382, 2557, 3000, 3483
Mox20I TGGCCA 2 cut(s) 5, 334
Mph1103I ATGCAT 2 cut(s) 262, 1494
MroXI GAANNNNTTC 3 cut(s) 201, 288, 1182
MscI TGGCCA 2 cut(s) 5, 334
MslI CAYNNNNRTG 3 cut(s) 1216, 1581, 3432
Msp20I TGGCCA 2 cut(s) 5, 334
MspA1I CMGCKG 1 cut(s) 3285
MspI CCGG 6 cut(s) 51, 590, 963, 1069, 2346, 3016
MspR9I CCNGG 6 cut(s) 987, 1069, 1551, 2195, 2531, 3398
MunI CAATTG 4 cut(s) 11, 702, 938, 1763
Mva1269I GAATGC 3 cut(s) 1284, 2697, 2930
MvaI CCWGG 5 cut(s) 987, 1551, 2195, 2531, 3398
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 4 cut(s) 1498, 1546, 2360, 3568
NciI CCSGG 1 cut(s) 1069
NlaIV GGNNCC 8 cut(s) 354, 838, 2029, 2062, 2101, 2173, 2581, 3270
NmeAIII GCCGAG 1 cut(s) 3637
NmuCI GTSAC 3 cut(s) 2629, 2952, 3914
NsiI ATGCAT 2 cut(s) 262, 1494
NspI RCATGY 2 cut(s) 622, 1478
NspV TTCGAA 1 cut(s) 3056
PaeR7I CTCGAG 1 cut(s) 496
PagI TCATGA 2 cut(s) 1081, 1528
PceI AGGCCT 1 cut(s) 1182
PciSI GCTCTTC 1 cut(s) 1106
PcsI WCGNNNNNNNCGW 1 cut(s) 2940
PctI GAATGC 3 cut(s) 1284, 2697, 2930
PdmI GAANNNNTTC 3 cut(s) 201, 288, 1182
PflMI CCANNNNNTGG 1 cut(s) 1569
PfoI TCCNGGA 1 cut(s) 2529
PinAI ACCGGT 1 cut(s) 50
PleI GAGTC 8 cut(s) 1543, 1680, 2346, 2388, 3123, 3204, 3596, 3622
PpsI GAGTC 8 cut(s) 1543, 1680, 2346, 2388, 3123, 3204, 3596, 3622
Ppu21I YACGTR 1 cut(s) 3582
PshBI ATTAAT 1 cut(s) 2673
PsiI TTATAA 2 cut(s) 1001, 1715
Psp124BI GAGCTC 1 cut(s) 1101
Psp6I CCWGG 5 cut(s) 985, 1549, 2193, 2529, 3396
PspGI CCWGG 5 cut(s) 985, 1549, 2193, 2529, 3396
PspN4I GGNNCC 8 cut(s) 354, 838, 2029, 2062, 2101, 2173, 2581, 3270
PspPI GGNCC 6 cut(s) 837, 943, 983, 1066, 1456, 3418
PstI CTGCAG 1 cut(s) 3886
PvuII CAGCTG 1 cut(s) 3285
RsaI GTAC 3 cut(s) 1938, 2068, 3406
RsaNI GTAC 3 cut(s) 1937, 2067, 3405
RseI CAYNNNNRTG 3 cut(s) 1216, 1581, 3432
SacI GAGCTC 1 cut(s) 1101
SalI GTCGAC 1 cut(s) 800
SapI GCTCTTC 1 cut(s) 1106
Sau96I GGNCC 6 cut(s) 837, 943, 983, 1066, 1456, 3418
SchI GAGTC 8 cut(s) 1544, 1681, 2347, 2389, 3123, 3204, 3597, 3623
ScrFI CCNGG 6 cut(s) 987, 1069, 1551, 2195, 2531, 3398
SduI GDGCHC 2 cut(s) 1101, 2903
SexAI ACCWGGT 1 cut(s) 3396
SfcI CTRYAG 2 cut(s) 1230, 3882
Sfr274I CTCGAG 1 cut(s) 496
SfuI TTCGAA 1 cut(s) 3056
SinI GGWCC 3 cut(s) 943, 983, 3418
SlaI CTCGAG 1 cut(s) 496
SmiMI CAYNNNNRTG 3 cut(s) 1216, 1581, 3432
SmlI CTYRAG 4 cut(s) 496, 1669, 2335, 2377
SmoI CTYRAG 4 cut(s) 496, 1669, 2335, 2377
SseBI AGGCCT 1 cut(s) 1182
SsiI CCGC 6 cut(s) 501, 886, 1768, 1793, 1858, 2967
SspI AATATT 2 cut(s) 1398, 3605
SspMI CTAG 9 cut(s) 833, 1011, 1076, 1431, 1502, 1934, 2057, 2232, 3888
SstI GAGCTC 1 cut(s) 1101
StuI AGGCCT 1 cut(s) 1182
StyD4I CCNGG 6 cut(s) 985, 1067, 1549, 2193, 2529, 3396
StyI CCWWGG 2 cut(s) 298, 1279
TaaI ACNGT 7 cut(s) 228, 727, 1731, 2811, 3276, 3315, 3501
TaiI ACGT 5 cut(s) 366, 1722, 1870, 3584, 3669
TatI WGTACW 2 cut(s) 1936, 2066
TauI GCSGC 2 cut(s) 1860, 2970
TscAI CASTG 6 cut(s) 1244, 1323, 2279, 2356, 3281, 3709
TseFI GTSAC 3 cut(s) 2629, 2952, 3914
Tsp45I GTSAC 3 cut(s) 2629, 2952, 3914
TspGWI ACGGA 1 cut(s) 3674
TspRI CASTG 6 cut(s) 1244, 1323, 2279, 2356, 3281, 3709
Van91I CCANNNNNTGG 1 cut(s) 1569
VpaK11BI GGWCC 3 cut(s) 943, 983, 3418
VspI ATTAAT 1 cut(s) 2673
XagI CCTNNNNNAGG 1 cut(s) 1278
XceI RCATGY 2 cut(s) 622, 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 740
XhoI CTCGAG 1 cut(s) 496
XmiI GTMKAC 1 cut(s) 801
XmnI GAANNNNTTC 3 cut(s) 201, 288, 1182
XspI CTAG 9 cut(s) 833, 1011, 1076, 1431, 1502, 1934, 2057, 2232, 3888
Zsp2I ATGCAT 2 cut(s) 262, 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.