MD10G1164000.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010915
Physical Location & Seq
Reverse (-)
25484062 .. 25488600
4539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1164000.v1.1.491

Sequence Viewer

Length: 2712 bp
ATGGACCTCCATTGTTTCTGGGTTGGCATCTGTCTGTTCTTTGGCTTTTCGCTAGTTGCCTTATGTGACCAAGATGGTTTCTTGAGCTTAACTTGCGGTGGAACTGCCGATTACACCGATTCATCTTCAATCTCATGGATTCCAGACAGTGCATACATCACCACAGGCAACACAACCAGTGTTGATTACATTGAAGGAACCTCCTCATCTAGTGTCCCGGTTCGGTACTTCCCAATTTCCCAAGGCCGGAAATGTTACAAGCTACCAGTCACCAATGTGTCATCTTTGGTTCTTGTGAGAGCTCAGTTTGTATATAAAAACTATGACGGGCATCGAAGGCCCCCGTCATTCTCCGTCTCTCTTGGGACAGCCATTGTTAGTACTATAGATCTCCTAAAAAATGATCCGTGGACAGAAGAGTTCGTTTGGCCAGTTGACAAGGACATGGTGTCGTTCTGTTTGCACGAAATTGCAGGTAGAGGAGCTCCTGTAATTTCTACACTTGAAGTTAGGCCACTTCCTCAAGAAGCTTACACAAGTGGCATGGAAGATTTCCCTAACAAGTCACTTAGGAAAAACTACCGGATAAATTGCGGTTACACGAATGGAACTTTGAGGTATCCTTTGGATCCGTATGATCGGATATGGGATGCTGATCAAAGCTTTTCGCCATTTCATACGTCCACCGAGTTCAAGACTCAGCTTAGCTTCAATTTTTCTGCTTTGAAGGAGGCTCCCCCTGCTGCCATTCTCCAAACAGCAAGAGTTTTGGCGCGAAGGACCATGTTGACATATACCTTTCCTCTTGATACCCTGGCAGACTACTACATTGTCCTCTATTTTGCTGGGATTCTTCCTGTTTTTCCTTCGTTCGATATCTTAATCAACGGTGCTGTTGTGCAGTCAAACTATACCATCAGAAGCTCAGAAGTTAGAACACTATATTTTAAACAGAGAGGAATAATGAGCTTGAACGTCACACTGAAGACCATCAGCTTCTATCCTCAAGTCAACGCAATCGAGGTGTATGAAATTCTCGATGTTCCAGAGGAAGCCTCCTCCACTACAGTATCAGCTCTTCAGGTTATCCAACAGTCTACCGGTTTAGATTTGGGGTGGCAAGATGATCCTTGCTCTCCTAAATCATGGGATCAAATTGGATGCGAAGGAAACATAGTCACATCACTGGAGCTTCCGGGAATCAGTTTGAGGTCGATTAGTGCAGCCATCGGCGATTTGCTTGATCTTAAAACATTGGATCTGCATAACACATCACTTGCTGGTGAAATACAGAATTTGGGCAGCTTGACACGCCTCGAGAAATTGAATCTGAGCTTCAATCGGCTAACATCCTTCGGTACTGAGTTGGAGAACTTGGTTAGCCTCCAAATTCTGGACTTGCAAAATAATACTTTGCAGGGAATCGTTCCAGACAGCTTGGGAGAGTTAGAAGAACTTCACTTATTGAACCTGGAAAACAACAAACTACAAGGCACTCTTCCGCTGTCCTTGAACCGAGAAAGTTTGGAAATCAGGACATCTGGAAATTTATGCCTCTCTTTTTCGACAATGTCATGCAATGATCCTTCATCCAACTCTTCAATTGAGGCACCACAAGTTACACTCTTTCCCCGAAAGAAACACACTGAACACAGCCGTGTAGCAATTATACTTGGCGCAATTGGAGGAGCCTTACTTGCACTTGCGATATTTGTCTTCCTTCTAGTATTCTTGCACGTGAAGAAAAGGAGAACTGAAATGACTTGCGCGGCAAGGGCGGTGGCGGATATGCGAAACTGGAATGCTGCAAGAGTTTTTACCTACAAAGAAATCAAGGCAGCTACAAACAAGTTTAAGGAGGTTCTAGGCCGGGGTAGTTTCGGTTGTGTTTACCTTGGAAAGCTTTCGGATGGAAAAATGGTGGCTGTGAAAGTACGATTCGATAAAAGCCAACTCGGGGCAGATTCTTTTATCAACGAGGTGCGTCTCTTGTCGGGAATTAGCCATCAAAATCTTGTAGGCTTGGAAGGATTCTGTCATGAAGCAAAGCAGCAAATACTTGTTTATGAGTATCTTCCTGGCGGATCGTTGGCTAACCATCTTTATGGTCCAAAGAGTAAAAAAGTTTCAATGAGTTGGGTTCGCCGGCTGAAAATTGCTGTCGATGCTGCAAAAGGATTGGACTATCTACACAACGGGAATGAGCCGCGAGTCATACACCGTGATGTGAAATGCAGTAACATCCTTCTGGACAAGGAGATGAATGCTAAGGTTTGTGATTTTGGCCTTTCTAAGCAAGTAATGCAAGCGGAAGCAACTCATGTGACCACTATCGTTAAGGGCACTGCCGGCTATATTGACCCTGAATATTACTCAACCCAGCAGCTTACTGAGAAAAGTGATGTCTATAGCTTTGGAGTTGTTCTTCTGGAGCTGATATGCGGGAGAGAACCATTGAGTCACTCTGGAACTCCAGACTCCTTTAACCTGGTGTTATGGGCCAAGCCTTACTTACAGGCAGGTGCATATGAGATAGTGGATGAGAGCCTGCAGGGCACATTTGATGTCGAAAGCATGAGAAAGGCAGCATTAGTTGCTATAAAATCTGTGGAGAGAGATGCATCGCAAAGGCCAACCATAGCAGAGGTTTTGGCCGAGCTCAAAGAAGCCTTCAGCATTCAGCTTTCGTATCTCGCATCCCGTGACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

904

Amino Acids

100.11

Weight (kDa)

5.83

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 31 - 344 2e-54 Malectin-like domain
LRR_8 PF13855 438 - 496 6.7e-09 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 617 - 883 5.1e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 618 - 885 2.9e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010915)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48740
fragaria_vesca FvH4_2g19020 FvH4_2g19020 FvH4_2g19020 FvH4_2g19020
malus_domestica MD10G1164000.v1.1
prunus_persica Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1
pyrus_communis pycom10g14140
rosa_chinensis RchiOBHm_Chr6g0284821
rosa_laevigata RLG00000012713
rosa_multiflora Rmu_sc0000686.1_g000012
rosa_roxburghii Rroxscaffold_7G00184340
rosa_rugosa Rorug06G0167200
rosa_samantha Rh6AG279900 Rh6BG279900 Rh6CG282100 Rh6DG275000
rosa_wichuraiana Rw6G024060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2510
Acc36I ACCTGC 2 cut(s) 464, 2510
AccB1I GGYRCC 1 cut(s) 1609
AccB7I CCANNNNNTGG 1 cut(s) 1393
AccI GTMKAC 1 cut(s) 1097
AccII CGCG 3 cut(s) 775, 1769, 2209
AclWI GGATC 8 cut(s) 398, 623, 636, 1121, 1158, 1266, 1577, 2092
AcoI YGGCCR 2 cut(s) 428, 2652
AcsI RAATTY 4 cut(s) 1032, 1294, 1389, 1546
AcuI CTGAAG 3 cut(s) 1004, 1064, 2656
AcvI CACGTG 1 cut(s) 1738
AfaI GTAC 4 cut(s) 227, 382, 1360, 1935
AfiI CCNNNNNNNGG 3 cut(s) 246, 1393, 1957
AflIII ACRYGT 1 cut(s) 2706
AgeI ACCGGT 1 cut(s) 1100
AhdI GACNNNNNGTC 1 cut(s) 448
AjnI CCWGG 4 cut(s) 813, 1470, 2077, 2487
AleI CACNNNNGTG 2 cut(s) 275, 1656
Alw21I GWGCWC 3 cut(s) 304, 487, 2661
Alw26I GTCTC 2 cut(s) 361, 1991
AlwI GGATC 8 cut(s) 398, 623, 636, 1121, 1158, 1266, 1577, 2092
Ama87I CYCGRG 2 cut(s) 1316, 1955
AoxI GGCC 9 cut(s) 244, 338, 428, 512, 1867, 2284, 2499, 2630, 2652
ApeKI GCWGC 9 cut(s) 743, 1223, 1302, 1805, 1838, 2050, 2168, 2383, 2585
ApoI RAATTY 4 cut(s) 1032, 1294, 1389, 1546
AsiGI ACCGGT 1 cut(s) 1100
Asp700I GAANNNNTTC 2 cut(s) 1455, 1903
AspLEI GCGC 3 cut(s) 775, 1679, 1769
AspS9I GGNCC 5 cut(s) 4, 339, 780, 2108, 2499
AsuC2I CCSGG 3 cut(s) 218, 1197, 1871
AsuHPI GGTGA 3 cut(s) 151, 262, 1295
AvaI CYCGRG 2 cut(s) 1316, 1955
AvaII GGWCC 3 cut(s) 4, 780, 2108
BaeGI GKGCMC 2 cut(s) 2345, 2558
BaeI ACNNNNGTAYC 2 cut(s) 1053, 1086
BalI TGGCCA 1 cut(s) 430
BamHI GGATCC 1 cut(s) 628
BanI GGYRCC 1 cut(s) 1609
BanII GRGCYC 3 cut(s) 304, 487, 2661
BarI GAAGNNNNNNTAC 2 cut(s) 1062, 1094
BbrPI CACGTG 1 cut(s) 1738
BbsI GAAGAC 2 cut(s) 992, 1708
Bbv12I GWGCWC 3 cut(s) 304, 487, 2661
BbvI GCAGC 9 cut(s) 730, 1235, 1314, 1792, 1850, 2062, 2155, 2395, 2597
BccI CCATC 7 cut(s) 68, 923, 998, 1235, 1904, 2013, 2106
BceAI ACGGC 1 cut(s) 1641
BciT130I CCWGG 4 cut(s) 815, 1472, 2079, 2489
BciVI GTATCC 1 cut(s) 630
BclI TGATCA 1 cut(s) 655
BcnI CCSGG 3 cut(s) 218, 1197, 1871
BcoDI GTCTC 2 cut(s) 361, 1991
BfaI CTAG 4 cut(s) 53, 210, 1724, 1865
BfmI CTRYAG 4 cut(s) 384, 1065, 2407, 2549
BfuAI ACCTGC 2 cut(s) 464, 2510
BfuI GTATCC 1 cut(s) 630
BglI GCCNNNNNGGC 1 cut(s) 2553
BglII AGATCT 1 cut(s) 388
BlpI GCTNAGC 1 cut(s) 704
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 7 cut(s) 218, 815, 1197, 1472, 1871, 2079, 2489
Bme18I GGWCC 3 cut(s) 4, 780, 2108
BmeRI GACNNNNNGTC 1 cut(s) 448
BmeT110I CYCGRG 2 cut(s) 1316, 1955
BmgT120I GGNCC 5 cut(s) 4, 339, 780, 2108, 2499
BmiI GGNNCC 6 cut(s) 199, 341, 630, 735, 1611, 1690
BmrFI CCNGG 7 cut(s) 218, 815, 1197, 1472, 1871, 2079, 2489
BmsI GCATC 8 cut(s) 36, 340, 640, 1151, 2155, 2608, 2630, 2705
BpiI GAAGAC 2 cut(s) 992, 1708
BplI GAGNNNNNCTC 2 cut(s) 1040, 1072
BpmI CTGGAG 3 cut(s) 1208, 2450, 2457
Bpu10I CCTNAGC 1 cut(s) 2268
Bpu1102I GCTNAGC 1 cut(s) 704
BpuEI CTTGAG 3 cut(s) 103, 507, 990
BpuMI CCSGG 3 cut(s) 218, 1197, 1871
BsaAI YACGTR 1 cut(s) 1738
BsaBI GATNNNNATC 1 cut(s) 654
BsaJI CCNNGG 5 cut(s) 241, 407, 813, 1870, 1894
BsaWI WCCGGW 2 cut(s) 582, 1100
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 3 cut(s) 246, 1393, 1957
Bse118I RCCGGY 3 cut(s) 1100, 2145, 2348
Bse1I ACTGG 5 cut(s) 177, 266, 431, 1191, 1802
Bse3DI GCAATG 1 cut(s) 1585
Bse8I GATNNNNATC 1 cut(s) 654
BseBI CCWGG 4 cut(s) 815, 1472, 2079, 2489
BseDI CCNNGG 5 cut(s) 241, 407, 813, 1870, 1894
BseGI GGATG 8 cut(s) 655, 1166, 1349, 1589, 1915, 2241, 2545, 2696
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 3 cut(s) 246, 1393, 1957
BseMI GCAATG 1 cut(s) 1585
BseMII CTCAG 6 cut(s) 317, 713, 939, 1322, 1353, 2382
BseNI ACTGG 5 cut(s) 177, 266, 431, 1191, 1802
BseRI GAGGAG 4 cut(s) 193, 495, 1048, 1701
BseSI GKGCMC 2 cut(s) 2345, 2558
BseXI GCAGC 9 cut(s) 730, 1235, 1314, 1792, 1850, 2062, 2155, 2395, 2597
BseYI CCCAGC 2 cut(s) 845, 2379
BsgI GTGCAG 2 cut(s) 920, 1242
Bsh1236I CGCG 3 cut(s) 775, 1769, 2209
BshFI GGCC 9 cut(s) 246, 340, 430, 514, 1869, 2286, 2501, 2632, 2654
BshNI GGYRCC 1 cut(s) 1609
BshTI ACCGGT 1 cut(s) 1100
BsiHKAI GWGCWC 3 cut(s) 304, 487, 2661
BsiHKCI CYCGRG 2 cut(s) 1316, 1955
BsiSI CCGG 8 cut(s) 218, 247, 583, 1101, 1196, 1870, 2146, 2349
BslFI GGGAC 2 cut(s) 200, 379
BslI CCNNNNNNNGG 3 cut(s) 246, 1393, 1957
BsmAI GTCTC 2 cut(s) 361, 1991
BsmBI CGTCTC 2 cut(s) 361, 1991
BsmFI GGGAC 2 cut(s) 200, 379
BsmI GAATGC 3 cut(s) 1807, 2269, 2676
BsnI GGCC 9 cut(s) 246, 340, 430, 514, 1869, 2286, 2501, 2632, 2654
BsoBI CYCGRG 2 cut(s) 1316, 1955
Bsp1286I GDGCHC 5 cut(s) 304, 487, 2345, 2558, 2661
Bsp1720I GCTNAGC 1 cut(s) 704
BspANI GGCC 9 cut(s) 246, 340, 430, 514, 1869, 2286, 2501, 2632, 2654
BspCNI CTCAG 6 cut(s) 316, 712, 938, 1323, 1354, 2383
BspFNI CGCG 3 cut(s) 775, 1769, 2209
BspHI TCATGA 1 cut(s) 2038
BspLI GGNNCC 6 cut(s) 199, 341, 630, 735, 1611, 1690
BspMAI CTGCAG 1 cut(s) 2553
BspMI ACCTGC 2 cut(s) 464, 2510
BspPI GGATC 8 cut(s) 398, 623, 636, 1121, 1158, 1266, 1577, 2092
BspQI GCTCTTC 1 cut(s) 1083
BspT107I GGYRCC 1 cut(s) 1609
BsrDI GCAATG 1 cut(s) 1585
BsrFI RCCGGY 3 cut(s) 1100, 2145, 2348
BsrI ACTGG 5 cut(s) 177, 266, 431, 1191, 1802
BssAI RCCGGY 3 cut(s) 1100, 2145, 2348
BssECI CCNNGG 5 cut(s) 241, 407, 813, 1870, 1894
BssT1I CCWWGG 2 cut(s) 241, 1894
Bst2UI CCWGG 4 cut(s) 815, 1472, 2079, 2489
Bst4CI ACNGT 5 cut(s) 149, 890, 1069, 1095, 2222
Bst6I CTCTTC 4 cut(s) 411, 1083, 1503, 1603
BstAPI GCANNNNNTGC 2 cut(s) 2302, 2594
BstBAI YACGTR 1 cut(s) 1738
BstC8I GCNNGC 4 cut(s) 2147, 2307, 2350, 2549
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 8 cut(s) 655, 1166, 1349, 1589, 1915, 2241, 2545, 2696
BstFNI CGCG 3 cut(s) 775, 1769, 2209
BstHHI GCGC 3 cut(s) 775, 1679, 1769
BstMAI GTCTC 2 cut(s) 361, 1991
BstNI CCWGG 4 cut(s) 815, 1472, 2079, 2489
BstNSI RCATGY 1 cut(s) 2710
BstSCI CCNGG 7 cut(s) 216, 813, 1195, 1470, 1869, 2077, 2487
BstSFI CTRYAG 4 cut(s) 384, 1065, 2407, 2549
BstSLI GKGCMC 2 cut(s) 2345, 2558
BstUI CGCG 3 cut(s) 775, 1769, 2209
BstV1I GCAGC 9 cut(s) 730, 1235, 1314, 1792, 1850, 2062, 2155, 2395, 2597
BstV2I GAAGAC 2 cut(s) 992, 1708
BstX2I RGATCY 3 cut(s) 388, 628, 1258
BstXI CCANNNNNNTGG 1 cut(s) 2105
BstYI RGATCY 3 cut(s) 388, 628, 1258
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 9 cut(s) 246, 340, 430, 514, 1869, 2286, 2501, 2632, 2654
BtgI CCRYGG 1 cut(s) 407
BtgZI GCGATG 1 cut(s) 2607
BtsCI GGATG 8 cut(s) 655, 1166, 1349, 1589, 1915, 2241, 2545, 2696
BtsI GCAGTG 1 cut(s) 2343
BtsIMutI CAGTG 6 cut(s) 154, 184, 980, 1184, 1644, 2343
BveI ACCTGC 2 cut(s) 464, 2510
Cac8I GCNNGC 4 cut(s) 2147, 2307, 2350, 2549
CciI TCATGA 1 cut(s) 2038
CfoI GCGC 3 cut(s) 775, 1679, 1769
Cfr10I RCCGGY 3 cut(s) 1100, 2145, 2348
Cfr13I GGNCC 5 cut(s) 4, 339, 780, 2108, 2499
CseI GACGC 1 cut(s) 1973
CsiI ACCWGGT 1 cut(s) 2487
Csp6I GTAC 4 cut(s) 226, 381, 1359, 1934
CspAI ACCGGT 1 cut(s) 1100
CspCI CAANNNNNGTGG 2 cut(s) 1761, 1796
CviQI GTAC 4 cut(s) 226, 381, 1359, 1934
DraI TTTAAA 1 cut(s) 949
DriI GACNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 2 cut(s) 428, 2652
Eam1104I CTCTTC 4 cut(s) 411, 1083, 1503, 1603
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 4 cut(s) 411, 1083, 1503, 1603
EciI GGCGGA 2 cut(s) 1799, 2097
Ecl136II GAGCTC 3 cut(s) 302, 485, 2659
Eco130I CCWWGG 2 cut(s) 241, 1894
Eco24I GRGCYC 3 cut(s) 304, 487, 2661
Eco32I GATATC 1 cut(s) 877
Eco47I GGWCC 3 cut(s) 4, 780, 2108
Eco53kI GAGCTC 3 cut(s) 302, 485, 2659
Eco57I CTGAAG 3 cut(s) 1004, 1064, 2656
Eco72I CACGTG 1 cut(s) 1738
Eco88I CYCGRG 2 cut(s) 1316, 1955
EcoICRI GAGCTC 3 cut(s) 302, 485, 2659
EcoO109I RGGNCCY 1 cut(s) 339
EcoRII CCWGG 4 cut(s) 813, 1470, 2077, 2487
EcoRV GATATC 1 cut(s) 877
EcoT14I CCWWGG 2 cut(s) 241, 1894
EcoT22I ATGCAT 1 cut(s) 2623
EcoT38I GRGCYC 3 cut(s) 304, 487, 2661
ErhI CCWWGG 2 cut(s) 241, 1894
Esp3I CGTCTC 2 cut(s) 361, 1991
FalI AAGNNNNNCTT 4 cut(s) 1482, 1514, 2495, 2527
FaqI GGGAC 2 cut(s) 200, 379
FauI CCCGC 1 cut(s) 2435
FauNDI CATATG 1 cut(s) 2527
FbaI TGATCA 1 cut(s) 655
FblI GTMKAC 1 cut(s) 1097
FokI GGATG 8 cut(s) 662, 1173, 1336, 1576, 1922, 2228, 2552, 2683
FriOI GRGCYC 3 cut(s) 304, 487, 2661
FspBI CTAG 4 cut(s) 53, 210, 1724, 1865
GlaI GCGC 3 cut(s) 774, 1678, 1768
GsaI CCCAGC 2 cut(s) 849, 2383
GsuI CTGGAG 3 cut(s) 1208, 2450, 2457
HaeIII GGCC 9 cut(s) 246, 340, 430, 514, 1869, 2286, 2501, 2632, 2654
HapII CCGG 8 cut(s) 218, 247, 583, 1101, 1196, 1870, 2146, 2349
HgaI GACGC 1 cut(s) 1973
HhaI GCGC 3 cut(s) 775, 1679, 1769
Hin6I GCGC 3 cut(s) 773, 1677, 1767
HinP1I GCGC 3 cut(s) 773, 1677, 1767
HincII GTYRAC 3 cut(s) 436, 789, 1012
HindII GTYRAC 3 cut(s) 436, 789, 1012
HindIII AAGCTT 3 cut(s) 528, 661, 1901
HpaII CCGG 8 cut(s) 218, 247, 583, 1101, 1196, 1870, 2146, 2349
HphI GGTGA 3 cut(s) 151, 262, 1295
Hpy166II GTNNAC 7 cut(s) 411, 436, 684, 789, 1012, 1098, 1891
Hpy188I TCNGA 5 cut(s) 642, 920, 928, 1332, 1909
Hpy8I GTNNAC 7 cut(s) 411, 436, 684, 789, 1012, 1098, 1891
HpyCH4III ACNGT 5 cut(s) 149, 890, 1069, 1095, 2222
HpyCH4IV ACGT 3 cut(s) 680, 975, 1737
HpySE526I ACGT 3 cut(s) 680, 975, 1737
HspAI GCGC 3 cut(s) 773, 1677, 1767
KroI GCCGGC 2 cut(s) 2145, 2348
KroNI GCCGGC 2 cut(s) 2147, 2350
Ksp22I TGATCA 1 cut(s) 655
LguI GCTCTTC 1 cut(s) 1083
LmnI GCTCC 6 cut(s) 482, 490, 739, 1189, 1688, 2431
Lsp1109I GCAGC 9 cut(s) 730, 1235, 1314, 1792, 1850, 2062, 2155, 2395, 2597
LweI GCATC 8 cut(s) 36, 340, 640, 1151, 2155, 2608, 2630, 2705
MabI ACCWGGT 1 cut(s) 2487
MaeI CTAG 4 cut(s) 53, 210, 1724, 1865
MaeII ACGT 3 cut(s) 680, 975, 1737
MfeI CAATTG 2 cut(s) 1602, 1680
MflI RGATCY 3 cut(s) 388, 628, 1258
MhlI GDGCHC 5 cut(s) 304, 487, 2345, 2558, 2661
MlsI TGGCCA 1 cut(s) 430
MluNI TGGCCA 1 cut(s) 430
MlyI GAGTC 4 cut(s) 691, 2220, 2467, 2471
MmeI TCCRAC 3 cut(s) 1114, 1347, 1617
Mox20I TGGCCA 1 cut(s) 430
Mph1103I ATGCAT 1 cut(s) 2623
MroNI GCCGGC 2 cut(s) 2145, 2348
MroXI GAANNNNTTC 2 cut(s) 1455, 1903
MscI TGGCCA 1 cut(s) 430
MseI TTAA 7 cut(s) 89, 881, 948, 1248, 1854, 2337, 2484
MslI CAYNNNNRTG 4 cut(s) 275, 1656, 2103, 2223
Msp20I TGGCCA 1 cut(s) 430
MspA1I CMGCKG 1 cut(s) 1504
MspI CCGG 8 cut(s) 218, 247, 583, 1101, 1196, 1870, 2146, 2349
MspR9I CCNGG 7 cut(s) 218, 815, 1197, 1472, 1871, 2079, 2489
MunI CAATTG 2 cut(s) 1602, 1680
Mva1269I GAATGC 3 cut(s) 1807, 2269, 2676
MvaI CCWGG 4 cut(s) 815, 1472, 2079, 2489
MvnI CGCG 3 cut(s) 775, 1769, 2209
NaeI GCCGGC 2 cut(s) 2147, 2350
NciI CCSGG 3 cut(s) 218, 1197, 1871
NdeI CATATG 1 cut(s) 2527
NgoMIV GCCGGC 2 cut(s) 2145, 2348
NlaIV GGNNCC 6 cut(s) 199, 341, 630, 735, 1611, 1690
NmeAIII GCCGAG 1 cut(s) 2680
NmuCI GTSAC 8 cut(s) 65, 268, 564, 976, 1177, 2323, 2459, 2702
NsiI ATGCAT 1 cut(s) 2623
NspI RCATGY 1 cut(s) 2710
OliI CACNNNNGTG 2 cut(s) 275, 1656
PaeR7I CTCGAG 1 cut(s) 1316
PagI TCATGA 1 cut(s) 2038
PaqCI CACCTGC 1 cut(s) 2510
PciI ACATGT 1 cut(s) 2706
PciSI GCTCTTC 1 cut(s) 1083
PctI GAATGC 3 cut(s) 1807, 2269, 2676
PdiI GCCGGC 2 cut(s) 2147, 2350
PdmI GAANNNNTTC 2 cut(s) 1455, 1903
PfeI GAWTC 9 cut(s) 119, 139, 850, 1200, 1327, 1422, 1938, 1964, 2031
PflFI GACNNNGTC 1 cut(s) 1570
PflMI CCANNNNNTGG 1 cut(s) 1393
PfoI TCCNGGA 1 cut(s) 1195
PinAI ACCGGT 1 cut(s) 1100
PleI GAGTC 4 cut(s) 691, 2219, 2466, 2471
PmaCI CACGTG 1 cut(s) 1738
PmlI CACGTG 1 cut(s) 1738
PpsI GAGTC 4 cut(s) 691, 2219, 2466, 2471
Ppu21I YACGTR 1 cut(s) 1738
PscI ACATGT 1 cut(s) 2706
Psp124BI GAGCTC 3 cut(s) 304, 487, 2661
Psp6I CCWGG 4 cut(s) 813, 1470, 2077, 2487
PspCI CACGTG 1 cut(s) 1738
PspFI CCCAGC 2 cut(s) 845, 2379
PspGI CCWGG 4 cut(s) 813, 1470, 2077, 2487
PspN4I GGNNCC 6 cut(s) 199, 341, 630, 735, 1611, 1690
PspPI GGNCC 5 cut(s) 4, 339, 780, 2108, 2499
PstI CTGCAG 1 cut(s) 2553
PsuI RGATCY 3 cut(s) 388, 628, 1258
PsyI GACNNNGTC 1 cut(s) 1570
RsaI GTAC 4 cut(s) 227, 382, 1360, 1935
RsaNI GTAC 4 cut(s) 226, 381, 1359, 1934
RseI CAYNNNNRTG 4 cut(s) 275, 1656, 2103, 2223
SacI GAGCTC 3 cut(s) 304, 487, 2661
SapI GCTCTTC 1 cut(s) 1083
SaqAI TTAA 7 cut(s) 89, 881, 948, 1248, 1854, 2337, 2484
Sau96I GGNCC 5 cut(s) 4, 339, 780, 2108, 2499
SbfI CCTGCAGG 1 cut(s) 2553
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 4 cut(s) 691, 2220, 2467, 2471
ScrFI CCNGG 7 cut(s) 218, 815, 1197, 1472, 1871, 2079, 2489
SdaI CCTGCAGG 1 cut(s) 2553
SduI GDGCHC 5 cut(s) 304, 487, 2345, 2558, 2661
SexAI ACCWGGT 1 cut(s) 2487
SfaNI GCATC 8 cut(s) 36, 340, 640, 1151, 2155, 2608, 2630, 2705
SfcI CTRYAG 4 cut(s) 384, 1065, 2407, 2549
Sfr274I CTCGAG 1 cut(s) 1316
SinI GGWCC 3 cut(s) 4, 780, 2108
SlaI CTCGAG 1 cut(s) 1316
SmiMI CAYNNNNRTG 4 cut(s) 275, 1656, 2103, 2223
SmlI CTYRAG 4 cut(s) 82, 522, 1005, 1316
SmoI CTYRAG 4 cut(s) 82, 522, 1005, 1316
Sse8387I CCTGCAGG 1 cut(s) 2553
SspI AATATT 1 cut(s) 2369
SspMI CTAG 4 cut(s) 53, 210, 1724, 1865
SstI GAGCTC 3 cut(s) 304, 487, 2661
StyD4I CCNGG 7 cut(s) 216, 813, 1195, 1470, 1869, 2077, 2487
StyI CCWWGG 2 cut(s) 241, 1894
TaaI ACNGT 5 cut(s) 149, 890, 1069, 1095, 2222
TaiI ACGT 3 cut(s) 683, 978, 1740
TatI WGTACW 1 cut(s) 380
TauI GCSGC 2 cut(s) 1772, 2209
TfiI GAWTC 9 cut(s) 119, 139, 850, 1200, 1327, 1422, 1938, 1964, 2031
Tru1I TTAA 7 cut(s) 89, 881, 948, 1248, 1854, 2337, 2484
Tru9I TTAA 7 cut(s) 89, 881, 948, 1248, 1854, 2337, 2484
TscAI CASTG 6 cut(s) 154, 184, 987, 1191, 1651, 2350
TseFI GTSAC 8 cut(s) 65, 268, 564, 976, 1177, 2323, 2459, 2702
TseI GCWGC 9 cut(s) 743, 1223, 1302, 1805, 1838, 2050, 2168, 2383, 2585
Tsp45I GTSAC 8 cut(s) 65, 268, 564, 976, 1177, 2323, 2459, 2702
TspDTI ATGAA 6 cut(s) 111, 665, 1044, 1578, 2055, 2276
TspGWI ACGGA 3 cut(s) 343, 396, 621
TspRI CASTG 6 cut(s) 154, 184, 987, 1191, 1651, 2350
Tth111I GACNNNGTC 1 cut(s) 1570
Van91I CCANNNNNTGG 1 cut(s) 1393
VpaK11BI GGWCC 3 cut(s) 4, 780, 2108
XapI RAATTY 4 cut(s) 1032, 1294, 1389, 1546
XceI RCATGY 1 cut(s) 2710
XhoI CTCGAG 1 cut(s) 1316
XmiI GTMKAC 1 cut(s) 1097
XmnI GAANNNNTTC 2 cut(s) 1455, 1903
XspI CTAG 4 cut(s) 53, 210, 1724, 1865
ZrmI AGTACT 1 cut(s) 382
Zsp2I ATGCAT 1 cut(s) 2623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.