RchiOBHm_Chr6g0284821

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010915
Physical Location & Seq
Reverse (-)
48128861 .. 48133699
4839 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25544

Sequence Viewer

Length: 2721 bp
ATGGACCTTCTGTGTTTTTGGGTTGGCTTCTTTCTGTTCCTTGGATTTTGGGTAGTTGCTTTCTCTGATCAAGATGGTTTCTTGAGCTTAACTTGTGGTGGAACTACGAATTACACCGATTCCTCCAATATCTCATGGATTCCAGACAATGAATTCATAGCCACTGGAAAGACCACTACCATCAGTTACATTGAGGGGACCTCCTCATTTAGTGTCCCGGTTCGGTTTTTCCCAATTCTCCAATCTCACAACTGTTATAGGCTACCAGTGAGTAATGTGTCATTTTTGGTTCTTGTTAGAGCTCAATTTGTGTACAAAGACTATGATGGACTTGGGAAACCCCCATCATTCTCTGTATCTCTTGGCACAGCCATTGTTAGTACTATAGATCTCAGAAAAACCGATCCTTGGATAGAAGAGTTTTTATGGCCAGTTGATAAGGACACAGTCTCTTTTTGTTTGCATAATATACCAGATAGTGGGGTTCCTGTAATTACTTCAATTGAAGTTAGGCCACTTCCCCAAGGAGCTTACACAAGTGGCATGGAAGACTTCCCACATAAGTCACTAAGGAAAAGTTATCGAATAGATTGTGGCAACACAAATGGATCCTTGAGGTATCCGTTGGATCCATATGATCGTATATGGGATGCTGATCAAAGCTTTGCACCATTTCATGTATCAACTGGTTTTAAGACTCAACTTATTACCTTCAATTTTTTGGGGTTAAAGGAGAGTCTCCCAGCTGATATCCTTCAAACTGCAAGAGTTTTGGCACGGAGGGATGCCTTGACATATAACTTATCTCTTGACACCTTGGCAGACTACTACATTGTCCTCTACTTTGCTGGGATTCTGCCTGTGTTTCCTTCATTCGACATCTTGATTAATGGTGATGTTGCACAATCGGATTATACTGTAAGAAGCTCAGAAGTTAGTGCACTATACTTCACGCAGAGAGGAATAAACAGCTTGAACATCACACTTAAGAGCATCAGCTTCTATCCTCAAATCAATGCAATTGAGGTGTATGAAATTCTTGATATTCCAGAAGAAGCCTCCTCCACCACAGTTTCAGCCCTTCAGGTTATCCAACAGTCAATTGGTTTAGATTTGGGATGGCAAGGTGACCCTTGCTCTCCGACAAAATGGGATCAAGTTGAATGTGAAGGAAACATAGTTACCTCATTGAAGCTTTCAGACATCAACTTGAGGTCAATTAGTCCAGCCATTGGTGACTTGCTGGATCTTAAAACACTGGATTTGCATAACACATCGCTTACCGGTGAACTACAGAATCTGGGTAGCTTGACACGTCTTGAGAAACTGAATCTGAGCTTCAATCAGTTAACTTCCTTTGGAACTGAGTTGGACAGCTTGGTTAGCCTTCAAATTCTGGACTTGCAAAACAATACTTTACAGGGAATGGTTCCGGATAGCTTGGGAGAGTTGGAGGATCTTCACTTGTTGAATGTAGAAAATAACAAACTGCAAGGTGTTCTTCCCCAGTCTTTGAACACAGAAAGCTTGGAAATTAGAGCATCCGGAAATCTGTGCCTATCTTTCTCAACAATGACATGCAATGATTTCTCAGGCTCAGACAACTCTTCTATTGAGGCACCACAAGTTACTATCTTTCCCGGAAAGAAACACACGAGGCACAGTAATATAGCAGTTATACTTGGTGCTATTGGAGGAGCCTTACTCGCCCTGCTGATACTTGCCACCATTTCTGTAATGTTGTATGTGAAGAAAAGAAGAACTGATGTCACATCAATGGAGAGGGCAATGTTAGATATGCGTAACTGGAATTCAGCAAAGGTTTTTAGCCACAAAGAAATTAAAGCAGCCACCAACAATTTCAAGCAACTTTTAGGCCGTGGAAGTTTTGGTTCAGTTTACCTTGGAAAACTTCCCAATGGAAGAATGGTGGCTGTGAAAGTGCGATTTGATAAAAGCCAACTTGGGGCTGATTCCTTTGTCAATGAGGTGCATCTCTTGTCTCGAATTCGCCATCAAAATCTTGTATGCTTGGAAGGATTCTGTCATGAATCAAAGCAGCAAATACTTGTTTACGAGTATCTTCATTGTGGTTCGTTGGCTGACCACCTTTATGGTCCAAAGAGTAAAAAAGTTTCACTGAGCTGGGTTCGCCGACTGAAAATTGCTGTTGATGCTGCAAAAGGATTGGACTATCTACACAATGGAAATGAACCACGAATCATACACCGTGATATAAAATGCAGCAACATACTTTTGGACAAGCAGATGAATGCTAAGGTTTGTGATTTTGGCCTCTCTAAGCAAGTAACGCAGGCAGATGCAACCCACGTGACCACTGTTGTCAAGGGAACTGCTGGCTATCTTGATCCTGAATATTACTCAACACAACAGCTTACTGAGAAAAGTGATGTCTATAGCTTCGGAGTTGTTCTTCTGGAGCTCATTTGTGGAAGAGAACCATTAAGTCACTCAGGCACTCCAGACTCCTTTAATTTGGTGTTATGGGCCAAGCCTTATTTACAAGCAGGTGCTTACGAAATAGTGGATGAGAGCCTAGAGGGAACATTTGATGTTGAAAGCATGAGAAAGGCAGCCCTGGTTGCTGTAAGGTCCGTCGAAAGAGATGCATCAAAAAGACCAACCATAGCAGAGGTTTTGTCTGAGCTGAAAGAAGCCTATATCATTCAGCTATCATATCTCCAATCTCGCGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

906

Amino Acids

100.68

Weight (kDa)

5.44

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 31 - 346 6.3e-51 Malectin-like domain
LRR_4 PF12799 417 - 455 8.9e-06 Leucine Rich repeats (2 copies)
LRR_4 PF12799 438 - 473 6.9e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 439 - 497 1.1e-06 Leucine rich repeat
Pkinase PF00069 621 - 888 1.4e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 621 - 837 2.6e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010915)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48740
fragaria_vesca FvH4_2g19020 FvH4_2g19020 FvH4_2g19020 FvH4_2g19020
malus_domestica MD10G1164000.v1.1
prunus_persica Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1
pyrus_communis pycom10g14140
rosa_chinensis RchiOBHm_Chr6g0284821
rosa_laevigata RLG00000012713
rosa_multiflora Rmu_sc0000686.1_g000012
rosa_roxburghii Rroxscaffold_7G00184340
rosa_rugosa Rorug06G0167200
rosa_samantha Rh6AG279900 Rh6BG279900 Rh6CG282100 Rh6DG275000
rosa_wichuraiana Rw6G024060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2519
Acc36I ACCTGC 1 cut(s) 2519
AccB1I GGYRCC 1 cut(s) 1618
AccB7I CCANNNNNTGG 2 cut(s) 479, 1232
AccII CGCG 1 cut(s) 2712
AccIII TCCGGA 2 cut(s) 1432, 1544
AclWI GGATC 9 cut(s) 398, 603, 616, 623, 636, 1161, 1254, 1464, 2363
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 5 cut(s) 152, 1035, 1392, 1810, 2007
AcuI CTGAAG 1 cut(s) 1067
AcvI CACGTG 1 cut(s) 2332
AfaI GTAC 2 cut(s) 314, 382
AfiI CCNNNNNNNGG 4 cut(s) 408, 479, 1232, 1966
AflII CTTAAG 1 cut(s) 986
AflIII ACRYGT 1 cut(s) 1313
AgeI ACCGGT 1 cut(s) 1283
AjiI CACGTC 1 cut(s) 1316
AjnI CCWGG 1 cut(s) 2598
Alw21I GWGCWC 3 cut(s) 304, 943, 2445
Alw26I GTCTC 3 cut(s) 454, 743, 2007
Alw44I GTGCAC 1 cut(s) 939
AlwI GGATC 9 cut(s) 398, 603, 616, 623, 636, 1161, 1254, 1464, 2363
AlwNI CAGNNNCTG 1 cut(s) 1300
Aor13HI TCCGGA 2 cut(s) 1432, 1544
AoxI GGCC 5 cut(s) 428, 512, 1876, 2293, 2508
ApaLI GTGCAC 1 cut(s) 939
ApeKI GCWGC 5 cut(s) 1847, 2059, 2177, 2244, 2594
ApoI RAATTY 5 cut(s) 152, 1035, 1392, 1810, 2007
AseI ATTAAT 1 cut(s) 888
AsiGI ACCGGT 1 cut(s) 1283
AspS9I GGNCC 5 cut(s) 4, 198, 2117, 2508, 2613
AsuC2I CCSGG 2 cut(s) 218, 1641
AsuHPI GGTGA 4 cut(s) 905, 1139, 1247, 1298
AvaII GGWCC 4 cut(s) 4, 198, 2117, 2613
BaeGI GKGCMC 1 cut(s) 943
BalI TGGCCA 1 cut(s) 430
BamHI GGATCC 2 cut(s) 608, 628
BanI GGYRCC 1 cut(s) 1618
BanII GRGCYC 2 cut(s) 304, 2445
BauI CACGAG 1 cut(s) 1654
BbrPI CACGTG 1 cut(s) 2332
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 3 cut(s) 304, 943, 2445
BbvI GCAGC 5 cut(s) 1859, 2071, 2164, 2256, 2606
BccI CCATC 6 cut(s) 68, 188, 320, 352, 1113, 2022
BceAI ACGGC 1 cut(s) 1863
BcgI CGANNNNNNTGC 2 cut(s) 2609, 2643
BciT130I CCWGG 1 cut(s) 2600
BciVI GTATCC 1 cut(s) 630
BclI TGATCA 2 cut(s) 67, 655
BcnI CCSGG 2 cut(s) 218, 1641
BcoDI GTCTC 3 cut(s) 454, 743, 2007
BfaI CTAG 1 cut(s) 2558
BfmI CTRYAG 3 cut(s) 384, 1292, 2416
BfrI CTTAAG 1 cut(s) 986
BfuAI ACCTGC 1 cut(s) 2519
BfuI GTATCC 1 cut(s) 630
BglII AGATCT 1 cut(s) 388
BisI GCNGC 5 cut(s) 1848, 2060, 2178, 2245, 2595
BlsI GCNGC 5 cut(s) 1849, 2061, 2179, 2246, 2596
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 3 cut(s) 218, 1641, 2600
Bme18I GGWCC 4 cut(s) 4, 198, 2117, 2613
BmgBI CACGTC 1 cut(s) 1316
BmgT120I GGNCC 5 cut(s) 4, 198, 2117, 2508, 2613
BmiI GGNNCC 7 cut(s) 199, 486, 610, 630, 1431, 1620, 1699
BmrFI CCNGG 3 cut(s) 218, 1641, 2600
BmrI ACTGGG 1 cut(s) 1501
BmsI GCATC 9 cut(s) 640, 775, 1002, 1550, 2002, 2164, 2311, 2617, 2639
BmuI ACTGGG 1 cut(s) 1501
BpiI GAAGAC 1 cut(s) 555
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 1689, 1721
BpmI CTGGAG 2 cut(s) 2459, 2466
Bpu10I CCTNAGC 1 cut(s) 2277
BpuEI CTTGAG 4 cut(s) 103, 634, 1231, 1340
BpuMI CCSGG 2 cut(s) 218, 1641
BsaAI YACGTR 1 cut(s) 2332
BsaBI GATNNNNATC 1 cut(s) 654
BsaJI CCNNGG 7 cut(s) 40, 407, 523, 816, 1879, 1903, 2598
BsaWI WCCGGW 3 cut(s) 1283, 1432, 1544
Bsc4I CCNNNNNNNGG 4 cut(s) 408, 479, 1232, 1966
Bse118I RCCGGY 1 cut(s) 1283
Bse1I ACTGG 7 cut(s) 169, 266, 431, 691, 1263, 1507, 1811
Bse3DI GCAATG 2 cut(s) 1588, 1794
Bse8I GATNNNNATC 1 cut(s) 654
BseAI TCCGGA 2 cut(s) 1432, 1544
BseBI CCWGG 1 cut(s) 2600
BseDI CCNNGG 7 cut(s) 40, 407, 523, 816, 1879, 1903, 2598
BseGI GGATG 5 cut(s) 655, 790, 1124, 1541, 2554
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 4 cut(s) 408, 479, 1232, 1966
BseMI GCAATG 2 cut(s) 1588, 1794
BseNI ACTGG 7 cut(s) 169, 266, 431, 691, 1263, 1507, 1811
BseRI GAGGAG 3 cut(s) 193, 1051, 1710
BseSI GKGCMC 1 cut(s) 943
BseXI GCAGC 5 cut(s) 1859, 2071, 2164, 2256, 2606
BseYI CCCAGC 3 cut(s) 742, 848, 2145
Bsh1236I CGCG 1 cut(s) 2712
BshFI GGCC 5 cut(s) 430, 514, 1878, 2295, 2510
BshNI GGYRCC 1 cut(s) 1618
BshTI ACCGGT 1 cut(s) 1283
BsiHKAI GWGCWC 3 cut(s) 304, 943, 2445
BsiSI CCGG 5 cut(s) 218, 1284, 1433, 1545, 1641
BslFI GGGAC 2 cut(s) 200, 211
BslI CCNNNNNNNGG 4 cut(s) 408, 479, 1232, 1966
BsmAI GTCTC 3 cut(s) 454, 743, 2007
BsmFI GGGAC 2 cut(s) 200, 211
BsmI GAATGC 1 cut(s) 2278
BsnI GGCC 5 cut(s) 430, 514, 1878, 2295, 2510
Bsp1286I GDGCHC 3 cut(s) 304, 943, 2445
Bsp13I TCCGGA 2 cut(s) 1432, 1544
Bsp1407I TGTACA 1 cut(s) 312
Bsp68I TCGCGA 1 cut(s) 2712
BspANI GGCC 5 cut(s) 430, 514, 1878, 2295, 2510
BspEI TCCGGA 2 cut(s) 1432, 1544
BspFNI CGCG 1 cut(s) 2712
BspHI TCATGA 1 cut(s) 2047
BspLI GGNNCC 7 cut(s) 199, 486, 610, 630, 1431, 1620, 1699
BspMI ACCTGC 1 cut(s) 2519
BspPI GGATC 9 cut(s) 398, 603, 616, 623, 636, 1161, 1254, 1464, 2363
BspT107I GGYRCC 1 cut(s) 1618
BspTI CTTAAG 1 cut(s) 986
BsrDI GCAATG 2 cut(s) 1588, 1794
BsrFI RCCGGY 1 cut(s) 1283
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 7 cut(s) 169, 266, 431, 691, 1263, 1507, 1811
BssAI RCCGGY 1 cut(s) 1283
BssECI CCNNGG 7 cut(s) 40, 407, 523, 816, 1879, 1903, 2598
BssSI CACGAG 1 cut(s) 1654
BssT1I CCWWGG 5 cut(s) 40, 407, 523, 816, 1903
Bst2BI CACGAG 1 cut(s) 1654
Bst2UI CCWGG 1 cut(s) 2600
Bst4CI ACNGT 8 cut(s) 254, 448, 919, 1072, 1098, 1664, 2231, 2341
Bst6I CTCTTC 3 cut(s) 411, 1612, 2449
BstAFI CTTAAG 1 cut(s) 986
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 1 cut(s) 2332
BstC8I GCNNGC 2 cut(s) 2316, 2359
BstDSI CCRYGG 1 cut(s) 1879
BstEII GGTNACC 1 cut(s) 1127
BstF5I GGATG 5 cut(s) 655, 790, 1124, 1541, 2554
BstFNI CGCG 1 cut(s) 2712
BstMAI GTCTC 3 cut(s) 454, 743, 2007
BstMWI GCNNNNNNNGC 6 cut(s) 1383, 1706, 2151, 2174, 2311, 2603
BstNI CCWGG 1 cut(s) 2600
BstNSI RCATGY 1 cut(s) 1581
BstPI GGTNACC 1 cut(s) 1127
BstSCI CCNGG 3 cut(s) 216, 1639, 2598
BstSFI CTRYAG 3 cut(s) 384, 1292, 2416
BstSLI GKGCMC 1 cut(s) 943
BstUI CGCG 1 cut(s) 2712
BstV1I GCAGC 5 cut(s) 1859, 2071, 2164, 2256, 2606
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 5 cut(s) 388, 608, 628, 1246, 1456
BstXI CCANNNNNNTGG 1 cut(s) 2114
BstYI RGATCY 5 cut(s) 388, 608, 628, 1246, 1456
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 5 cut(s) 430, 514, 1878, 2295, 2510
BtgI CCRYGG 1 cut(s) 1879
BtgZI GCGATG 1 cut(s) 1260
BtrI CACGTC 1 cut(s) 1316
BtsCI GGATG 5 cut(s) 655, 790, 1124, 1541, 2554
BtsIMutI CAGTG 5 cut(s) 162, 273, 1256, 2138, 2337
BtuMI TCGCGA 1 cut(s) 2712
BveI ACCTGC 1 cut(s) 2519
Cac8I GCNNGC 2 cut(s) 2316, 2359
CaiI CAGNNNCTG 1 cut(s) 1300
CciI TCATGA 1 cut(s) 2047
Cfr10I RCCGGY 1 cut(s) 1283
Cfr13I GGNCC 5 cut(s) 4, 198, 2117, 2508, 2613
Csp6I GTAC 2 cut(s) 313, 381
CspAI ACCGGT 1 cut(s) 1283
CviAII CATG 6 cut(s) 135, 544, 677, 1578, 2048, 2584
CviQI GTAC 2 cut(s) 313, 381
EaeI YGGCCR 1 cut(s) 428
Eam1104I CTCTTC 3 cut(s) 411, 1612, 2449
EarI CTCTTC 3 cut(s) 411, 1612, 2449
Ecl136II GAGCTC 2 cut(s) 302, 2443
Eco130I CCWWGG 5 cut(s) 40, 407, 523, 816, 1903
Eco24I GRGCYC 2 cut(s) 304, 2445
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 4 cut(s) 4, 198, 2117, 2613
Eco53kI GAGCTC 2 cut(s) 302, 2443
Eco57I CTGAAG 1 cut(s) 1067
Eco72I CACGTG 1 cut(s) 2332
Eco91I GGTNACC 1 cut(s) 1127
EcoICRI GAGCTC 2 cut(s) 302, 2443
EcoO109I RGGNCCY 1 cut(s) 198
EcoO65I GGTNACC 1 cut(s) 1127
EcoRI GAATTC 3 cut(s) 152, 1810, 2007
EcoRII CCWGG 1 cut(s) 2598
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 5 cut(s) 40, 407, 523, 816, 1903
EcoT22I ATGCAT 1 cut(s) 2632
EcoT38I GRGCYC 2 cut(s) 304, 2445
ErhI CCWWGG 5 cut(s) 40, 407, 523, 816, 1903
FaeI CATG 6 cut(s) 138, 547, 680, 1581, 2051, 2587
FalI AAGNNNNNCTT 2 cut(s) 1485, 1517
FaqI GGGAC 2 cut(s) 200, 211
FatI CATG 6 cut(s) 134, 543, 676, 1577, 2047, 2583
FauNDI CATATG 1 cut(s) 634
FbaI TGATCA 2 cut(s) 67, 655
Fnu4HI GCNGC 5 cut(s) 1848, 2060, 2178, 2245, 2595
FokI GGATG 5 cut(s) 662, 797, 1131, 1528, 2561
FriOI GRGCYC 2 cut(s) 304, 2445
Fsp4HI GCNGC 5 cut(s) 1848, 2060, 2178, 2245, 2595
FspBI CTAG 1 cut(s) 2558
GluI GCNGC 5 cut(s) 1848, 2060, 2178, 2245, 2595
GsaI CCCAGC 3 cut(s) 746, 852, 2149
GsuI CTGGAG 2 cut(s) 2459, 2466
HaeIII GGCC 5 cut(s) 430, 514, 1878, 2295, 2510
HapII CCGG 5 cut(s) 218, 1284, 1433, 1545, 1641
Hin1II CATG 6 cut(s) 138, 547, 680, 1581, 2051, 2587
HincII GTYRAC 1 cut(s) 1350
HindII GTYRAC 1 cut(s) 1350
HindIII AAGCTT 3 cut(s) 661, 1193, 1525
HpaI GTTAAC 1 cut(s) 1350
HpaII CCGG 5 cut(s) 218, 1284, 1433, 1545, 1641
HphI GGTGA 4 cut(s) 905, 1139, 1247, 1298
Hpy166II GTNNAC 6 cut(s) 313, 941, 1289, 1350, 1900, 2074
Hpy8I GTNNAC 6 cut(s) 313, 941, 1289, 1350, 1900, 2074
Hpy99I CGWCG 1 cut(s) 2621
HpyAV CCTTC 8 cut(s) 17, 721, 764, 879, 1091, 1163, 1397, 2030
HpyCH4III ACNGT 8 cut(s) 254, 448, 919, 1072, 1098, 1664, 2231, 2341
HpyCH4IV ACGT 2 cut(s) 1315, 2331
HpyF10VI GCNNNNNNNGC 6 cut(s) 1383, 1706, 2151, 2174, 2311, 2603
HpySE526I ACGT 2 cut(s) 1315, 2331
Hsp92II CATG 6 cut(s) 138, 547, 680, 1581, 2051, 2587
Kpn2I TCCGGA 2 cut(s) 1432, 1544
Ksp22I TGATCA 2 cut(s) 67, 655
KspAI GTTAAC 1 cut(s) 1350
LmnI GCTCC 3 cut(s) 527, 1697, 2440
Lsp1109I GCAGC 5 cut(s) 1859, 2071, 2164, 2256, 2606
LweI GCATC 9 cut(s) 640, 775, 1002, 1550, 2002, 2164, 2311, 2617, 2639
MaeI CTAG 1 cut(s) 2558
MaeII ACGT 2 cut(s) 1315, 2331
MfeI CAATTG 3 cut(s) 501, 1020, 1101
MflI RGATCY 5 cut(s) 388, 608, 628, 1246, 1456
MhlI GDGCHC 3 cut(s) 304, 943, 2445
MlsI TGGCCA 1 cut(s) 430
MluNI TGGCCA 1 cut(s) 430
MlyI GAGTC 3 cut(s) 691, 745, 2480
MmeI TCCRAC 5 cut(s) 606, 1117, 1166, 1350, 1431
Mox20I TGGCCA 1 cut(s) 430
Mph1103I ATGCAT 1 cut(s) 2632
MroI TCCGGA 2 cut(s) 1432, 1544
MscI TGGCCA 1 cut(s) 430
MslI CAYNNNNRTG 2 cut(s) 1775, 2112
Msp20I TGGCCA 1 cut(s) 430
MspA1I CMGCKG 1 cut(s) 746
MspCI CTTAAG 1 cut(s) 986
MspI CCGG 5 cut(s) 218, 1284, 1433, 1545, 1641
MspR9I CCNGG 3 cut(s) 218, 1641, 2600
MunI CAATTG 3 cut(s) 501, 1020, 1101
Mva1269I GAATGC 1 cut(s) 2278
MvaI CCWGG 1 cut(s) 2600
MvnI CGCG 1 cut(s) 2712
MwoI GCNNNNNNNGC 6 cut(s) 1383, 1706, 2151, 2174, 2311, 2603
NciI CCSGG 2 cut(s) 218, 1641
NdeI CATATG 1 cut(s) 634
NlaIII CATG 6 cut(s) 138, 547, 680, 1581, 2051, 2587
NlaIV GGNNCC 7 cut(s) 199, 486, 610, 630, 1431, 1620, 1699
NmuCI GTSAC 6 cut(s) 564, 1127, 1235, 1768, 2332, 2468
NruI TCGCGA 1 cut(s) 2712
NsiI ATGCAT 1 cut(s) 2632
NspI RCATGY 1 cut(s) 1581
PagI TCATGA 1 cut(s) 2047
PaqCI CACCTGC 1 cut(s) 2519
PctI GAATGC 1 cut(s) 2278
PfeI GAWTC 9 cut(s) 119, 139, 853, 1297, 1330, 1973, 2040, 2051, 2220
PflFI GACNNNGTC 1 cut(s) 446
PflMI CCANNNNNTGG 2 cut(s) 479, 1232
PfoI TCCNGGA 1 cut(s) 1639
PinAI ACCGGT 1 cut(s) 1283
PkrI GCNGC 5 cut(s) 1849, 2061, 2179, 2246, 2596
PleI GAGTC 3 cut(s) 691, 744, 2480
PmaCI CACGTG 1 cut(s) 2332
PmlI CACGTG 1 cut(s) 2332
PpsI GAGTC 3 cut(s) 691, 744, 2480
Ppu21I YACGTR 1 cut(s) 2332
PpuMI RGGWCCY 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 888
Psp124BI GAGCTC 2 cut(s) 304, 2445
Psp5II RGGWCCY 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 2598
PspCI CACGTG 1 cut(s) 2332
PspEI GGTNACC 1 cut(s) 1127
PspFI CCCAGC 3 cut(s) 742, 848, 2145
PspGI CCWGG 1 cut(s) 2598
PspN4I GGNNCC 7 cut(s) 199, 486, 610, 630, 1431, 1620, 1699
PspPI GGNCC 5 cut(s) 4, 198, 2117, 2508, 2613
PspPPI RGGWCCY 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 1300
PsuI RGATCY 5 cut(s) 388, 608, 628, 1246, 1456
PsyI GACNNNGTC 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 746
RruI TCGCGA 1 cut(s) 2712
RsaI GTAC 2 cut(s) 314, 382
RsaNI GTAC 2 cut(s) 313, 381
RseI CAYNNNNRTG 2 cut(s) 1775, 2112
SacI GAGCTC 2 cut(s) 304, 2445
SatI GCNGC 5 cut(s) 1848, 2060, 2178, 2245, 2595
Sau96I GGNCC 5 cut(s) 4, 198, 2117, 2508, 2613
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 3 cut(s) 691, 745, 2480
ScrFI CCNGG 3 cut(s) 218, 1641, 2600
SduI GDGCHC 3 cut(s) 304, 943, 2445
SfaNI GCATC 9 cut(s) 640, 775, 1002, 1550, 2002, 2164, 2311, 2617, 2639
SfcI CTRYAG 3 cut(s) 384, 1292, 2416
SinI GGWCC 4 cut(s) 4, 198, 2117, 2613
SmiMI CAYNNNNRTG 2 cut(s) 1775, 2112
SmlI CTYRAG 5 cut(s) 82, 613, 986, 1210, 1319
SmoI CTYRAG 5 cut(s) 82, 613, 986, 1210, 1319
SspI AATATT 1 cut(s) 2378
SspMI CTAG 1 cut(s) 2558
SstI GAGCTC 2 cut(s) 304, 2445
StyD4I CCNGG 3 cut(s) 216, 1639, 2598
StyI CCWWGG 5 cut(s) 40, 407, 523, 816, 1903
TaaI ACNGT 8 cut(s) 254, 448, 919, 1072, 1098, 1664, 2231, 2341
TaiI ACGT 2 cut(s) 1318, 2334
TaqI TCGA 4 cut(s) 583, 876, 2005, 2619
TatI WGTACW 2 cut(s) 312, 380
TfiI GAWTC 9 cut(s) 119, 139, 853, 1297, 1330, 1973, 2040, 2051, 2220
TscAI CASTG 5 cut(s) 169, 273, 1263, 2145, 2344
TseFI GTSAC 6 cut(s) 564, 1127, 1235, 1768, 2332, 2468
TseI GCWGC 5 cut(s) 1847, 2059, 2177, 2244, 2594
Tsp45I GTSAC 6 cut(s) 564, 1127, 1235, 1768, 2332, 2468
TspDTI ATGAA 9 cut(s) 145, 165, 665, 861, 1047, 2064, 2075, 2226, 2285
TspGWI ACGGA 3 cut(s) 612, 793, 2605
TspRI CASTG 5 cut(s) 169, 273, 1263, 2145, 2344
Tth111I GACNNNGTC 1 cut(s) 446
Van91I CCANNNNNTGG 2 cut(s) 479, 1232
Vha464I CTTAAG 1 cut(s) 986
VneI GTGCAC 1 cut(s) 939
VpaK11BI GGWCC 4 cut(s) 4, 198, 2117, 2613
VspI ATTAAT 1 cut(s) 888
XapI RAATTY 5 cut(s) 152, 1035, 1392, 1810, 2007
XceI RCATGY 1 cut(s) 1581
XcmI CCANNNNNNNNNTGG 2 cut(s) 1100, 1924
XspI CTAG 1 cut(s) 2558
ZrmI AGTACT 1 cut(s) 382
Zsp2I ATGCAT 1 cut(s) 2632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.