Rw6G024060

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010915
Physical Location & Seq
Reverse (-)
46837977 .. 46842444
4468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G024060.1

Sequence Viewer

Length: 2661 bp
ATGGACCTTCTGTGTTTTTGGGTTGGCTTCTTTCTGTTCCTTGGATTTTGGGTAGTTGCTTTCTCTGATCAAGATGGTTTCTTGAGCTTAACTTGTGGTGGAACTAGGAATTACACCGATTCCTCCAATATCTCATGGATTCCAGACAATGAATTCATAGCCACTGGAAACACCACTACCATCAGTTACATTGAGGGGACCTCATTTAGTGTCCCGGTTCGGTTTTTCCCAATTCTCCAATCTCACAACTGTTATAGGCTACCAGTGAGTAATGTGTCATTTTTGGTTCTTGTTAGAGCTCAATTTGTGTACAAAGACTATGATGGACTTGGGAAACCCCCATCATTCTCTGTATCTCTTGGCACAGCCATTGTTAGTACTATAGATCTCAGAAAAACCGATCCTTGGATAGAAGAGTTTTTATGGCCAGTTGATAAGGACACAGTCTCTTTTTGTTTGCATAATATACCAGATAGTGGGGTTCCTGTAATTACTTCAATTGAAGTTAGGCCACTTCCCCAAGGAGCTTACACAAGTGGCATGGAAGATTTCCCACATAAGTCACTGAGGAAAAGTTATCGAATAGATTGTGGCAACACAAATGGATCCTTGAGGTATCCGTTGGATCCATATGATCGTATATGGGATGCTGATCAAAGCTTTGCACCATTTCATGTGTCAACTGCTGATATCCTTCAAACTGCGAGAGTTTTGGCACGGAGGGATGCCTTGACATATAACTTATCTCTTGACACCTTGGCAGACTACTACATTGTCCTCTACTTTGCTGGGATTCTGCCTGTGTTTCCTTCATTCGACATCTTGATTAATGGTGATGTTGCACAATCGAATTATACTGTAAGAAGCTCAGAAGTTAGTGCACTATACTTCACGCAGAGGGGAATAAACAGCTTGAACATCACACTTAAGAGCATCAGCTTCTATCCTCAAATCAATGCAATTGAGGTGTATGAAATTCTTGATATTCCAGAAGAAGCCTCCTCCACCACAGTTTCAGCTCTTCAGGTTATCCAACAGTCAATTGGTTTAGATTTGGGATGGCAAGGTGACCCTTGCTCTCCGACAAAATGGGATCAAGTTGAATGTGAAGGAAACATAGTTACCTCATTGAAGCTTTCAGATATCAACTTGAGGTCAATTAGTCCAGCCATTGGTGACTTGCTGGATCTTAAAACACTGGATTTGCATAACACATCGCTTACCGGTGAACTACAGAATCTGGGTAGCTTGACACGTCTTGAGAAACTGAATCTGAGCTTCAATCAGTTAACTTCATTTGGAACTGAGTTGGACAGCTTGGTTAGCCTTCAAATTCTGGACTTGCAAAACAATACTTTACAGGGAATGGTTCCGGATAGCTTGGGAGAGTTGGAGGATCTTCACTTGTTGAATGTAGAAAATAACAAACTGCAAGGTGTTCTTCCCCAGTCTTTGAACAGAGAAAGCTTGGAAATTAGAGCATCCGGAAATCTGTGCCTATCTTTCTCAACAATGACATGCAATGATTTCTCAGGCTCAGACAACTCTTCTATTGAGGCACCACAAGTTACTATCTTTCCCGGAAAGAAACACACGAGGCACAGTAATATAGCAGTTATACTTGGTGCTATTGGAGGAGCCTTACTTGCCCTGCTGATACTTGCCACCATTTCTGTAATGTTGTATGTGAAGAAAAGAAGAACTGATGTCACATCAATGGAGAGGGCAATGTTAGATATGCGTAACTGGAATTCAGCAAAGGTTTTTAGCCACAAAGAAATTAAAGCAGCCACCAACAATTTCAAGCAACTTTTAGGCCGTGGAAGTTTTGGTTCAGTTTACCTTGGAAAACTTCCCGATGGAAGAATGGTGGCTGTGAAAGTGCGATTTGATAAAAGCCAACTTGGGGCTGATTCCTTTGTCAATGAGGTGCATCTCTTGTCTCGAATTCGCCATCAAAATCTTGTATGCTTGGAAGGATTCTGTCATGAATCAAAGCAGCAAATACTTGTTTACGAGTATCTTCATTGTGGTTCGTTGGCTGACCACCTTTATGGTCCAAAGAGTAAAAAAGTTTCACTGAGCTGGGTTCGCCGACTGAAAATTGCTGTTGATGCTGCAAAAGGATTGGACTATCTACACAATGGAAATGAACCACGAATCATACACCGTGATATAAAATGCAGTAACATACTTTTGGACAAGCAGATGAATGCTAAGGTTTGTGATTTTGGCCTCTCTAAGCAAGTAATGCAGGCAGATGCAACTCACGTCACCACTGTTGTCAAGGGAACTGCTGGCTATCTTGATCCTGAATATTACTCTACACAACAGCTTACTGAGAAAAGCGATGTCTATAGCTTCGGAGTAGTTCTTCTGGAGCTCATTTGTGGAAGAGAACCATTGAGTCACTCAGGAACTCCAGACTCCTTTAATTTGGTGTTATGGGCCAAGCCTTACTTACAAGCAGGTGCTTATGAAATAGTGGATGAGAGCCTAGAGGGAACGTTTGATGTTGAAAGCATGAGAAAGGCAGCCCTAGTTGCTGTAAGGTCCGTAGAAAGAGATGCATCAAAAAGACCAACCATAGCAGAGGTTTTGGCTGAGCTGAAAGAAGCCTATATCATTCAGCTTTCATATCTCCAATCTCGCGACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

886

Amino Acids

98.57

Weight (kDa)

5.44

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 31 - 326 5.5e-45 Malectin-like domain
LRR_4 PF12799 397 - 435 8.7e-06 Leucine Rich repeats (2 copies)
LRR_4 PF12799 418 - 453 5.1e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 419 - 477 9.8e-07 Leucine rich repeat
Pkinase PF00069 601 - 868 3e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 601 - 806 3.9e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010915)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48740
fragaria_vesca FvH4_2g19020 FvH4_2g19020 FvH4_2g19020 FvH4_2g19020
malus_domestica MD10G1164000.v1.1
prunus_persica Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1
pyrus_communis pycom10g14140
rosa_chinensis RchiOBHm_Chr6g0284821
rosa_laevigata RLG00000012713
rosa_multiflora Rmu_sc0000686.1_g000012
rosa_roxburghii Rroxscaffold_7G00184340
rosa_rugosa Rorug06G0167200
rosa_samantha Rh6AG279900 Rh6BG279900 Rh6CG282100 Rh6DG275000
rosa_wichuraiana Rw6G024060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2459
Acc36I ACCTGC 1 cut(s) 2459
AccB1I GGYRCC 1 cut(s) 1558
AccB7I CCANNNNNTGG 2 cut(s) 476, 1172
AccII CGCG 1 cut(s) 2652
AccIII TCCGGA 2 cut(s) 1372, 1484
AclI AACGTT 1 cut(s) 2507
AclWI GGATC 9 cut(s) 395, 600, 613, 620, 633, 1101, 1194, 1404, 2303
AcoI YGGCCR 1 cut(s) 425
AcsI RAATTY 5 cut(s) 152, 975, 1332, 1750, 1947
AcuI CTGAAG 1 cut(s) 1007
AfaI GTAC 2 cut(s) 311, 379
AfiI CCNNNNNNNGG 4 cut(s) 405, 476, 1172, 1906
AflII CTTAAG 1 cut(s) 926
AflIII ACRYGT 1 cut(s) 1253
AgeI ACCGGT 1 cut(s) 1223
AjiI CACGTC 2 cut(s) 1256, 2272
Alw21I GWGCWC 3 cut(s) 301, 883, 2385
Alw26I GTCTC 2 cut(s) 451, 1947
Alw44I GTGCAC 1 cut(s) 879
AlwI GGATC 9 cut(s) 395, 600, 613, 620, 633, 1101, 1194, 1404, 2303
AlwNI CAGNNNCTG 1 cut(s) 1240
Aor13HI TCCGGA 2 cut(s) 1372, 1484
AoxI GGCC 5 cut(s) 425, 509, 1816, 2233, 2448
ApaLI GTGCAC 1 cut(s) 879
ApeKI GCWGC 4 cut(s) 1787, 1999, 2117, 2534
ApoI RAATTY 5 cut(s) 152, 975, 1332, 1750, 1947
AseI ATTAAT 1 cut(s) 828
AsiGI ACCGGT 1 cut(s) 1223
AspS9I GGNCC 5 cut(s) 4, 198, 2057, 2448, 2553
AsuC2I CCSGG 2 cut(s) 215, 1581
AsuHPI GGTGA 5 cut(s) 845, 1079, 1187, 1238, 2266
AvaII GGWCC 4 cut(s) 4, 198, 2057, 2553
BaeGI GKGCMC 1 cut(s) 883
BalI TGGCCA 1 cut(s) 427
BamHI GGATCC 2 cut(s) 605, 625
BanI GGYRCC 1 cut(s) 1558
BanII GRGCYC 2 cut(s) 301, 2385
BauI CACGAG 1 cut(s) 1594
Bbv12I GWGCWC 3 cut(s) 301, 883, 2385
BbvI GCAGC 4 cut(s) 1799, 2011, 2104, 2546
BccI CCATC 7 cut(s) 68, 188, 317, 349, 1053, 1853, 1962
BceAI ACGGC 1 cut(s) 1803
BciVI GTATCC 1 cut(s) 627
BclI TGATCA 2 cut(s) 67, 652
BcnI CCSGG 2 cut(s) 215, 1581
BcoDI GTCTC 2 cut(s) 451, 1947
BfaI CTAG 3 cut(s) 105, 2498, 2540
BfmI CTRYAG 3 cut(s) 381, 1232, 2356
BfrI CTTAAG 1 cut(s) 926
BfuAI ACCTGC 1 cut(s) 2459
BfuI GTATCC 1 cut(s) 627
BglII AGATCT 1 cut(s) 385
BisI GCNGC 4 cut(s) 1788, 2000, 2118, 2535
BlpI GCTNAGC 1 cut(s) 2604
BlsI GCNGC 4 cut(s) 1789, 2001, 2119, 2536
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 2 cut(s) 215, 1581
Bme18I GGWCC 4 cut(s) 4, 198, 2057, 2553
BmgBI CACGTC 2 cut(s) 1256, 2272
BmgT120I GGNCC 5 cut(s) 4, 198, 2057, 2448, 2553
BmiI GGNNCC 7 cut(s) 199, 483, 607, 627, 1371, 1560, 1639
BmrFI CCNGG 2 cut(s) 215, 1581
BmrI ACTGGG 1 cut(s) 1441
BmsI GCATC 9 cut(s) 637, 715, 942, 1490, 1942, 2104, 2251, 2557, 2579
BmuI ACTGGG 1 cut(s) 1441
BplI GAGNNNNNCTC 2 cut(s) 185, 217
BpmI CTGGAG 2 cut(s) 2399, 2406
Bpu10I CCTNAGC 1 cut(s) 2217
Bpu1102I GCTNAGC 1 cut(s) 2604
BpuEI CTTGAG 4 cut(s) 103, 631, 1171, 1280
BpuMI CCSGG 2 cut(s) 215, 1581
BsaBI GATNNNNATC 1 cut(s) 651
BsaJI CCNNGG 6 cut(s) 40, 404, 520, 756, 1819, 1843
BsaWI WCCGGW 3 cut(s) 1223, 1372, 1484
Bsc4I CCNNNNNNNGG 4 cut(s) 405, 476, 1172, 1906
Bse118I RCCGGY 1 cut(s) 1223
Bse1I ACTGG 6 cut(s) 169, 263, 428, 1203, 1447, 1751
Bse3DI GCAATG 2 cut(s) 1528, 1734
Bse8I GATNNNNATC 1 cut(s) 651
BseAI TCCGGA 2 cut(s) 1372, 1484
BseDI CCNNGG 6 cut(s) 40, 404, 520, 756, 1819, 1843
BseGI GGATG 5 cut(s) 652, 730, 1064, 1481, 2494
BseJI GATNNNNATC 1 cut(s) 651
BseLI CCNNNNNNNGG 4 cut(s) 405, 476, 1172, 1906
BseMI GCAATG 2 cut(s) 1528, 1734
BseNI ACTGG 6 cut(s) 169, 263, 428, 1203, 1447, 1751
BseRI GAGGAG 2 cut(s) 991, 1650
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 4 cut(s) 1799, 2011, 2104, 2546
BseYI CCCAGC 2 cut(s) 788, 2085
Bsh1236I CGCG 1 cut(s) 2652
BshFI GGCC 5 cut(s) 427, 511, 1818, 2235, 2450
BshNI GGYRCC 1 cut(s) 1558
BshTI ACCGGT 1 cut(s) 1223
BsiHKAI GWGCWC 3 cut(s) 301, 883, 2385
BsiSI CCGG 5 cut(s) 215, 1224, 1373, 1485, 1581
BslFI GGGAC 2 cut(s) 197, 211
BslI CCNNNNNNNGG 4 cut(s) 405, 476, 1172, 1906
BsmAI GTCTC 2 cut(s) 451, 1947
BsmFI GGGAC 2 cut(s) 197, 211
BsmI GAATGC 1 cut(s) 2218
BsnI GGCC 5 cut(s) 427, 511, 1818, 2235, 2450
Bsp1286I GDGCHC 3 cut(s) 301, 883, 2385
Bsp13I TCCGGA 2 cut(s) 1372, 1484
Bsp1407I TGTACA 1 cut(s) 309
Bsp1720I GCTNAGC 1 cut(s) 2604
Bsp68I TCGCGA 1 cut(s) 2652
BspANI GGCC 5 cut(s) 427, 511, 1818, 2235, 2450
BspEI TCCGGA 2 cut(s) 1372, 1484
BspFNI CGCG 1 cut(s) 2652
BspHI TCATGA 1 cut(s) 1987
BspLI GGNNCC 7 cut(s) 199, 483, 607, 627, 1371, 1560, 1639
BspMI ACCTGC 1 cut(s) 2459
BspPI GGATC 9 cut(s) 395, 600, 613, 620, 633, 1101, 1194, 1404, 2303
BspQI GCTCTTC 1 cut(s) 1026
BspT107I GGYRCC 1 cut(s) 1558
BspTI CTTAAG 1 cut(s) 926
BsrDI GCAATG 2 cut(s) 1528, 1734
BsrFI RCCGGY 1 cut(s) 1223
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 6 cut(s) 169, 263, 428, 1203, 1447, 1751
BssAI RCCGGY 1 cut(s) 1223
BssECI CCNNGG 6 cut(s) 40, 404, 520, 756, 1819, 1843
BssSI CACGAG 1 cut(s) 1594
BssT1I CCWWGG 5 cut(s) 40, 404, 520, 756, 1843
Bst2BI CACGAG 1 cut(s) 1594
Bst4CI ACNGT 8 cut(s) 251, 445, 859, 1012, 1038, 1604, 2171, 2281
Bst6I CTCTTC 4 cut(s) 408, 1026, 1552, 2389
BstAFI CTTAAG 1 cut(s) 926
BstAPI GCANNNNNTGC 1 cut(s) 2251
BstAUI TGTACA 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 2256, 2299
BstDSI CCRYGG 1 cut(s) 1819
BstEII GGTNACC 1 cut(s) 1067
BstF5I GGATG 5 cut(s) 652, 730, 1064, 1481, 2494
BstFNI CGCG 1 cut(s) 2652
BstMAI GTCTC 2 cut(s) 451, 1947
BstMWI GCNNNNNNNGC 6 cut(s) 1323, 1646, 2091, 2114, 2251, 2543
BstNSI RCATGY 1 cut(s) 1521
BstPI GGTNACC 1 cut(s) 1067
BstSCI CCNGG 2 cut(s) 213, 1579
BstSFI CTRYAG 3 cut(s) 381, 1232, 2356
BstSLI GKGCMC 1 cut(s) 883
BstUI CGCG 1 cut(s) 2652
BstV1I GCAGC 4 cut(s) 1799, 2011, 2104, 2546
BstX2I RGATCY 5 cut(s) 385, 605, 625, 1186, 1396
BstXI CCANNNNNNTGG 1 cut(s) 2054
BstYI RGATCY 5 cut(s) 385, 605, 625, 1186, 1396
BsuI GTATCC 1 cut(s) 627
BsuRI GGCC 5 cut(s) 427, 511, 1818, 2235, 2450
BtgI CCRYGG 1 cut(s) 1819
BtgZI GCGATG 2 cut(s) 1200, 2364
BtrI CACGTC 2 cut(s) 1256, 2272
BtsCI GGATG 5 cut(s) 652, 730, 1064, 1481, 2494
BtsIMutI CAGTG 6 cut(s) 162, 270, 563, 1196, 2078, 2277
BtuMI TCGCGA 1 cut(s) 2652
BveI ACCTGC 1 cut(s) 2459
Cac8I GCNNGC 2 cut(s) 2256, 2299
CaiI CAGNNNCTG 1 cut(s) 1240
CciI TCATGA 1 cut(s) 1987
Cfr10I RCCGGY 1 cut(s) 1223
Cfr13I GGNCC 5 cut(s) 4, 198, 2057, 2448, 2553
Csp6I GTAC 2 cut(s) 310, 378
CspAI ACCGGT 1 cut(s) 1223
CviAII CATG 6 cut(s) 135, 541, 674, 1518, 1988, 2524
CviQI GTAC 2 cut(s) 310, 378
EaeI YGGCCR 1 cut(s) 425
Eam1104I CTCTTC 4 cut(s) 408, 1026, 1552, 2389
EarI CTCTTC 4 cut(s) 408, 1026, 1552, 2389
Ecl136II GAGCTC 2 cut(s) 299, 2383
Eco130I CCWWGG 5 cut(s) 40, 404, 520, 756, 1843
Eco24I GRGCYC 2 cut(s) 301, 2385
Eco32I GATATC 2 cut(s) 691, 1144
Eco47I GGWCC 4 cut(s) 4, 198, 2057, 2553
Eco53kI GAGCTC 2 cut(s) 299, 2383
Eco57I CTGAAG 1 cut(s) 1007
Eco91I GGTNACC 1 cut(s) 1067
EcoICRI GAGCTC 2 cut(s) 299, 2383
EcoO109I RGGNCCY 1 cut(s) 198
EcoO65I GGTNACC 1 cut(s) 1067
EcoRI GAATTC 3 cut(s) 152, 1750, 1947
EcoRV GATATC 2 cut(s) 691, 1144
EcoT14I CCWWGG 5 cut(s) 40, 404, 520, 756, 1843
EcoT22I ATGCAT 1 cut(s) 2572
EcoT38I GRGCYC 2 cut(s) 301, 2385
ErhI CCWWGG 5 cut(s) 40, 404, 520, 756, 1843
FaeI CATG 6 cut(s) 138, 544, 677, 1521, 1991, 2527
FalI AAGNNNNNCTT 4 cut(s) 1425, 1457, 2444, 2476
FaqI GGGAC 2 cut(s) 197, 211
FatI CATG 6 cut(s) 134, 540, 673, 1517, 1987, 2523
FauNDI CATATG 2 cut(s) 631, 2657
FbaI TGATCA 2 cut(s) 67, 652
Fnu4HI GCNGC 4 cut(s) 1788, 2000, 2118, 2535
FokI GGATG 5 cut(s) 659, 737, 1071, 1468, 2501
FriOI GRGCYC 2 cut(s) 301, 2385
Fsp4HI GCNGC 4 cut(s) 1788, 2000, 2118, 2535
FspBI CTAG 3 cut(s) 105, 2498, 2540
GluI GCNGC 4 cut(s) 1788, 2000, 2118, 2535
GsaI CCCAGC 2 cut(s) 792, 2089
GsuI CTGGAG 2 cut(s) 2399, 2406
HaeIII GGCC 5 cut(s) 427, 511, 1818, 2235, 2450
HapII CCGG 5 cut(s) 215, 1224, 1373, 1485, 1581
Hin1II CATG 6 cut(s) 138, 544, 677, 1521, 1991, 2527
HincII GTYRAC 2 cut(s) 681, 1290
HindII GTYRAC 2 cut(s) 681, 1290
HindIII AAGCTT 3 cut(s) 658, 1133, 1465
HpaI GTTAAC 1 cut(s) 1290
HpaII CCGG 5 cut(s) 215, 1224, 1373, 1485, 1581
HphI GGTGA 5 cut(s) 845, 1079, 1187, 1238, 2266
Hpy166II GTNNAC 7 cut(s) 310, 681, 881, 1229, 1290, 1840, 2014
Hpy188I TCNGA 8 cut(s) 67, 392, 871, 1083, 1141, 1275, 1540, 2366
Hpy8I GTNNAC 7 cut(s) 310, 681, 881, 1229, 1290, 1840, 2014
HpyAV CCTTC 6 cut(s) 17, 704, 819, 1103, 1337, 1970
HpyCH4III ACNGT 8 cut(s) 251, 445, 859, 1012, 1038, 1604, 2171, 2281
HpyCH4IV ACGT 3 cut(s) 1255, 2271, 2507
HpyF10VI GCNNNNNNNGC 6 cut(s) 1323, 1646, 2091, 2114, 2251, 2543
HpySE526I ACGT 3 cut(s) 1255, 2271, 2507
Hsp92II CATG 6 cut(s) 138, 544, 677, 1521, 1991, 2527
Kpn2I TCCGGA 2 cut(s) 1372, 1484
Ksp22I TGATCA 2 cut(s) 67, 652
KspAI GTTAAC 1 cut(s) 1290
LguI GCTCTTC 1 cut(s) 1026
LmnI GCTCC 3 cut(s) 524, 1637, 2380
Lsp1109I GCAGC 4 cut(s) 1799, 2011, 2104, 2546
LweI GCATC 9 cut(s) 637, 715, 942, 1490, 1942, 2104, 2251, 2557, 2579
MaeI CTAG 3 cut(s) 105, 2498, 2540
MaeII ACGT 3 cut(s) 1255, 2271, 2507
MfeI CAATTG 3 cut(s) 498, 960, 1041
MflI RGATCY 5 cut(s) 385, 605, 625, 1186, 1396
MhlI GDGCHC 3 cut(s) 301, 883, 2385
MlsI TGGCCA 1 cut(s) 427
MluNI TGGCCA 1 cut(s) 427
MlyI GAGTC 2 cut(s) 2416, 2420
MmeI TCCRAC 5 cut(s) 603, 1057, 1106, 1290, 1371
Mox20I TGGCCA 1 cut(s) 427
Mph1103I ATGCAT 1 cut(s) 2572
MroI TCCGGA 2 cut(s) 1372, 1484
MscI TGGCCA 1 cut(s) 427
MseI TTAA 7 cut(s) 89, 828, 927, 1191, 1289, 1782, 2433
MslI CAYNNNNRTG 2 cut(s) 1715, 2052
Msp20I TGGCCA 1 cut(s) 427
MspCI CTTAAG 1 cut(s) 926
MspI CCGG 5 cut(s) 215, 1224, 1373, 1485, 1581
MspR9I CCNGG 2 cut(s) 215, 1581
MunI CAATTG 3 cut(s) 498, 960, 1041
Mva1269I GAATGC 1 cut(s) 2218
MvnI CGCG 1 cut(s) 2652
MwoI GCNNNNNNNGC 6 cut(s) 1323, 1646, 2091, 2114, 2251, 2543
NciI CCSGG 2 cut(s) 215, 1581
NdeI CATATG 2 cut(s) 631, 2657
NlaIII CATG 6 cut(s) 138, 544, 677, 1521, 1991, 2527
NlaIV GGNNCC 7 cut(s) 199, 483, 607, 627, 1371, 1560, 1639
NmuCI GTSAC 6 cut(s) 561, 1067, 1175, 1708, 2272, 2408
NruI TCGCGA 1 cut(s) 2652
NsiI ATGCAT 1 cut(s) 2572
NspI RCATGY 1 cut(s) 1521
PagI TCATGA 1 cut(s) 1987
PaqCI CACCTGC 1 cut(s) 2459
PciSI GCTCTTC 1 cut(s) 1026
PctI GAATGC 1 cut(s) 2218
PfeI GAWTC 9 cut(s) 119, 139, 793, 1237, 1270, 1913, 1980, 1991, 2160
PflFI GACNNNGTC 1 cut(s) 443
PflMI CCANNNNNTGG 2 cut(s) 476, 1172
PfoI TCCNGGA 1 cut(s) 1579
PinAI ACCGGT 1 cut(s) 1223
PkrI GCNGC 4 cut(s) 1789, 2001, 2119, 2536
PleI GAGTC 2 cut(s) 2415, 2420
PpsI GAGTC 2 cut(s) 2415, 2420
PpuMI RGGWCCY 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 828
Psp124BI GAGCTC 2 cut(s) 301, 2385
Psp1406I AACGTT 1 cut(s) 2507
Psp5II RGGWCCY 1 cut(s) 198
PspEI GGTNACC 1 cut(s) 1067
PspFI CCCAGC 2 cut(s) 788, 2085
PspN4I GGNNCC 7 cut(s) 199, 483, 607, 627, 1371, 1560, 1639
PspPI GGNCC 5 cut(s) 4, 198, 2057, 2448, 2553
PspPPI RGGWCCY 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 1240
PsuI RGATCY 5 cut(s) 385, 605, 625, 1186, 1396
PsyI GACNNNGTC 1 cut(s) 443
RruI TCGCGA 1 cut(s) 2652
RsaI GTAC 2 cut(s) 311, 379
RsaNI GTAC 2 cut(s) 310, 378
RseI CAYNNNNRTG 2 cut(s) 1715, 2052
SacI GAGCTC 2 cut(s) 301, 2385
SapI GCTCTTC 1 cut(s) 1026
SaqAI TTAA 7 cut(s) 89, 828, 927, 1191, 1289, 1782, 2433
SatI GCNGC 4 cut(s) 1788, 2000, 2118, 2535
Sau96I GGNCC 5 cut(s) 4, 198, 2057, 2448, 2553
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 2 cut(s) 2416, 2420
ScrFI CCNGG 2 cut(s) 215, 1581
SduI GDGCHC 3 cut(s) 301, 883, 2385
SfaNI GCATC 9 cut(s) 637, 715, 942, 1490, 1942, 2104, 2251, 2557, 2579
SfcI CTRYAG 3 cut(s) 381, 1232, 2356
SinI GGWCC 4 cut(s) 4, 198, 2057, 2553
SmiMI CAYNNNNRTG 2 cut(s) 1715, 2052
SmlI CTYRAG 5 cut(s) 82, 610, 926, 1150, 1259
SmoI CTYRAG 5 cut(s) 82, 610, 926, 1150, 1259
SspI AATATT 1 cut(s) 2318
SspMI CTAG 3 cut(s) 105, 2498, 2540
SstI GAGCTC 2 cut(s) 301, 2385
StyD4I CCNGG 2 cut(s) 213, 1579
StyI CCWWGG 5 cut(s) 40, 404, 520, 756, 1843
TaaI ACNGT 8 cut(s) 251, 445, 859, 1012, 1038, 1604, 2171, 2281
TaiI ACGT 3 cut(s) 1258, 2274, 2510
TaqI TCGA 4 cut(s) 580, 816, 848, 1945
TatI WGTACW 2 cut(s) 309, 377
TfiI GAWTC 9 cut(s) 119, 139, 793, 1237, 1270, 1913, 1980, 1991, 2160
Tru1I TTAA 7 cut(s) 89, 828, 927, 1191, 1289, 1782, 2433
Tru9I TTAA 7 cut(s) 89, 828, 927, 1191, 1289, 1782, 2433
TscAI CASTG 6 cut(s) 169, 270, 570, 1203, 2085, 2284
TseFI GTSAC 6 cut(s) 561, 1067, 1175, 1708, 2272, 2408
TseI GCWGC 4 cut(s) 1787, 1999, 2117, 2534
Tsp45I GTSAC 6 cut(s) 561, 1067, 1175, 1708, 2272, 2408
TspGWI ACGGA 3 cut(s) 609, 733, 2545
TspRI CASTG 6 cut(s) 169, 270, 570, 1203, 2085, 2284
Tth111I GACNNNGTC 1 cut(s) 443
Van91I CCANNNNNTGG 2 cut(s) 476, 1172
Vha464I CTTAAG 1 cut(s) 926
VneI GTGCAC 1 cut(s) 879
VpaK11BI GGWCC 4 cut(s) 4, 198, 2057, 2553
VspI ATTAAT 1 cut(s) 828
XapI RAATTY 5 cut(s) 152, 975, 1332, 1750, 1947
XceI RCATGY 1 cut(s) 1521
XcmI CCANNNNNNNNNTGG 1 cut(s) 1040
XspI CTAG 3 cut(s) 105, 2498, 2540
ZrmI AGTACT 1 cut(s) 379
Zsp2I ATGCAT 1 cut(s) 2572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.