Rroxscaffold_7G00184340

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010915
Physical Location & Seq
Forward (+)
23559724 .. 23564590
4867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184340.1

Sequence Viewer

Length: 2613 bp
ATGAATTACACCGATTCATCCAATATCTCATGGATTCCAGACAATGAATTCATAGCCACAGGCAACACCACTACCATCAGTTACATTGAGGGGACCTCATTTAGTGTCCCGGTTAGGTTTTTCCCAATTCTTCAATCCCACAACTGTTATAGGCTACCAGTGAGTAATGTGTCATTTTTGGTTCTTGTCAGAGCTCAATTTGTGTACAAAGACTATGATGGACTTGGGAAACCCCCTTCATTCTCTGTATCTCTTGGCACAGCCATTGTTAGTACTATAGATCTCAGAAAAACCGATCCTTGGATAGAAGAGTTTTTATGGCCAGTTGATAAGGACACAGTCTCTTTTTGTTTGCATAATATACCAGATAGAGGGGCTCCTGTAATTACTTCAATTGAAGTTAGGCCACTTCCCCAAGGAGCTTACACAAGTGGCATGGAAGATTTCCCACATAAGTCACTGAGGAAAAGTTATCGAATAGATTGTGGCAACACAAATGGATCCTTGAGGTATCCGTTGGATCCATATGATCGTATATGGGATGCTGATCAAAGCTTTGCACCATTTCATGTGTCAACTGGTTTTAAGACTCAACTTAATATCTTCAATGTTTGGGGGTTAAAGGAGAATCTCCCGGCTGATATCCTTCAAACCGCAAGAGTGTTGGCACGGAGGGATGTCTTGACATATAACTTATCTCTTGACACCTTGGCAGACTACTACATTGTCCTCTACTTTGCTGGGATTCTACCTGTGTTTCCTTCGTTCGACATCTTGATTAATGGTGATGTTGCACAATCAAATTATACTGTAAGAAGCTCAGAAGTTAGTGCACTATACTTCACGCAGAGAGCAATAAAGAGCTTGAACATCACACTTAAGAGCATCAGCTTCTATCCTCAAATCAATGCAATTGAGGTGTATGAAATTCTTGATATTCCAGAAGAAGCCTCCTCCACCACAGTTTCAGCCCTTCAGGTTATCCAACAGTCAATTGGTTTAGATTTGGGATGGCAAGGTGACCCTTGCTCTCCGACAAAATGGGATCAAGTTGAATGTGAAGGAAACATGGTTACCTCATTGAAGCTTTCAGACATCAACTTGAGGTCAATTAGTCCAGCCATTGGTGACTTGCTGGATCTTAAAACACTGGATTTGCATAACACATCGCTTACCGGTGAACTACAGAATCTGGGTAGCTTGACACGTCTTGAGAAACTGAATCTGAGCTTCAATCAGTTAACTTCCTTTGGAACTGAGTTGGACAGCTTGGTTAGCCTTCAAATTCTGGACTTGCAAAACAATACTTTACAGGGAATGGTTCCGGATAGCTTGGGAGAGTTAGAGGATCTTCACTTGTTGAATGTAGAAAATAACAAACTGCAAGGTGTTCTTCCCCAGTCTTTGAACACAGAAAGCTTGGAAATTAGAGCATCCGGAAATCTGTGCCTATCTTTCTCAACAATGACATGCAATGATTTCTCAGGCTCAGACAACTCTTCTATTGAGGCGCCACAAGTTACTATCTTTCCCGGAAAGAAACACACGAGGCACAGTAATATAGCAGTTATACTTGGTGCTATTGGAGGAGCCTTACTCGCCCTGCTGATACTTGCCTCCATTTCTGTAATGTTGTATGTGAAGAAAAGAAGAACTGATATCACATCAATGGAGAGGGCAATGTTAGATATGCGTAACTGGAATTCAGCAAAGGTTTTTAGCCACAAAGAAATTAAAGCAGCCACCAACAATTTCAAGCAACTTTTAGGCCGTGGAAGTTTTGGTTCAGTTTACCTTGGAAAACTTCCCAATGGAAGAATGGTGGCTGTGAAAGTGCGATTTGATAAAAGCCAACTTGGGGCTGATTCCTTTGTCAATGAGGTGCATCTCTTGTCTCGAATTCGCCATCAAAATCTTGTATGCTTGGAAGGATTCTGTCATGAATCAAAGCAGCAAATACTTGTTTACGAGTATCTTCATTGTGGTTCGTTGGCTGACCACCTTTATGGTCCAAAGAGTAAAAAAGTTTCACTGAGCTGGGTTCGCCGACTGAAAATTGCTGTTGATGCTGCAAAAGGATTGGACTATCTACACAATGGAAATGAACCACGAATCATACATCGTGATATAAAATGCAGTAACATACTTTTGGACAAGCAGATGAATGCTAAGGTTTGTGATTTTGGCCTCTCTAAGCAAGTAATGCGGCCAGATGCAACCCACGTGACCACTGTTGTCAAGGGAACTGCTGGCTATCTTGATCCTGAATATTACTCAACACAACAGCTTACTGAGAAAAGCGATGTCTATAGCTTCGGAGTAGTTCTTCTGGAGCTCATTTGTGGAAGAGAACCATTGAGTCACTCAGGCACTCCAGACTCCTTTAATTTGGTGTTATGGGCCAAGCCTTATTTACAAGCAGGTGCTTATGAAATAGTGGATGAGAGCCTAGAGGGAACATTTGATGTTGAAAGCATGAGAAAGGCAGCCCTAGTTGCTGTAAGGTCCGTAGAAAGAGATGCATCAAAAAGACCAACCATAGCAGAGGTTTTGGCTGAGCTGAAAGAAGCCTATAGCATTCAGCTATCATATCTCCAATCTCGAGACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

870

Amino Acids

96.89

Weight (kDa)

5.77

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 2 - 310 1.7e-50 Malectin-like domain
LRR_4 PF12799 381 - 419 6e-06 Leucine Rich repeats (2 copies)
LRR_4 PF12799 402 - 437 7.9e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 403 - 461 1e-06 Leucine rich repeat
Pkinase PF00069 585 - 852 3.2e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 585 - 790 8.7e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010915)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48740
fragaria_vesca FvH4_2g19020 FvH4_2g19020 FvH4_2g19020 FvH4_2g19020
malus_domestica MD10G1164000.v1.1
prunus_persica Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1 Prupe.8G202400_v2.0.a1
pyrus_communis pycom10g14140
rosa_chinensis RchiOBHm_Chr6g0284821
rosa_laevigata RLG00000012713
rosa_multiflora Rmu_sc0000686.1_g000012
rosa_roxburghii Rroxscaffold_7G00184340
rosa_rugosa Rorug06G0167200
rosa_samantha Rh6AG279900 Rh6BG279900 Rh6CG282100 Rh6DG275000
rosa_wichuraiana Rw6G024060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2411
Acc36I ACCTGC 1 cut(s) 2411
AccB1I GGYRCC 1 cut(s) 1510
AccB7I CCANNNNNTGG 1 cut(s) 1124
AccIII TCCGGA 2 cut(s) 1324, 1436
AciI CCGC 2 cut(s) 654, 2206
AclWI GGATC 9 cut(s) 290, 495, 508, 515, 528, 1053, 1146, 1356, 2255
AcoI YGGCCR 2 cut(s) 320, 2207
AcsI RAATTY 5 cut(s) 47, 927, 1284, 1702, 1899
AcuI CTGAAG 1 cut(s) 959
AcvI CACGTG 1 cut(s) 2224
AcyI GRCGYC 1 cut(s) 1511
AfaI GTAC 2 cut(s) 206, 274
AfiI CCNNNNNNNGG 4 cut(s) 300, 371, 1124, 1858
AflII CTTAAG 1 cut(s) 878
AflIII ACRYGT 1 cut(s) 1205
AgeI ACCGGT 1 cut(s) 1175
AjiI CACGTC 1 cut(s) 1208
Alw21I GWGCWC 3 cut(s) 196, 835, 2337
Alw26I GTCTC 3 cut(s) 346, 1899, 2598
Alw44I GTGCAC 1 cut(s) 831
AlwI GGATC 9 cut(s) 290, 495, 508, 515, 528, 1053, 1146, 1356, 2255
AlwNI CAGNNNCTG 1 cut(s) 1192
Ama87I CYCGRG 1 cut(s) 2601
Aor13HI TCCGGA 2 cut(s) 1324, 1436
AoxI GGCC 6 cut(s) 320, 404, 1768, 2185, 2207, 2400
ApaLI GTGCAC 1 cut(s) 831
ApeKI GCWGC 4 cut(s) 1739, 1951, 2069, 2486
ApoI RAATTY 5 cut(s) 47, 927, 1284, 1702, 1899
AseI ATTAAT 1 cut(s) 780
AsiGI ACCGGT 1 cut(s) 1175
AspLEI GCGC 1 cut(s) 1513
AspS9I GGNCC 4 cut(s) 93, 2009, 2400, 2505
AsuC2I CCSGG 3 cut(s) 110, 635, 1533
AsuHPI GGTGA 4 cut(s) 797, 1031, 1139, 1190
AvaI CYCGRG 1 cut(s) 2601
AvaII GGWCC 3 cut(s) 93, 2009, 2505
BaeGI GKGCMC 1 cut(s) 835
BalI TGGCCA 1 cut(s) 322
BamHI GGATCC 2 cut(s) 500, 520
BanI GGYRCC 1 cut(s) 1510
BanII GRGCYC 3 cut(s) 196, 379, 2337
BauI CACGAG 1 cut(s) 1546
BbrPI CACGTG 1 cut(s) 2224
Bbv12I GWGCWC 3 cut(s) 196, 835, 2337
BbvI GCAGC 4 cut(s) 1751, 1963, 2056, 2498
BccI CCATC 4 cut(s) 83, 212, 1005, 1914
BceAI ACGGC 1 cut(s) 1755
BciVI GTATCC 1 cut(s) 522
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 3 cut(s) 110, 635, 1533
BcoDI GTCTC 3 cut(s) 346, 1899, 2598
BfaI CTAG 2 cut(s) 2450, 2492
BfmI CTRYAG 4 cut(s) 276, 1184, 2308, 2572
BfoI RGCGCY 1 cut(s) 1514
BfrI CTTAAG 1 cut(s) 878
BfuAI ACCTGC 1 cut(s) 2411
BfuI GTATCC 1 cut(s) 522
BglII AGATCT 1 cut(s) 280
BisI GCNGC 5 cut(s) 1740, 1952, 2070, 2207, 2487
BlpI GCTNAGC 1 cut(s) 2556
BlsI GCNGC 5 cut(s) 1741, 1953, 2071, 2208, 2488
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 3 cut(s) 110, 635, 1533
Bme18I GGWCC 3 cut(s) 93, 2009, 2505
BmeT110I CYCGRG 1 cut(s) 2601
BmgBI CACGTC 1 cut(s) 1208
BmgT120I GGNCC 4 cut(s) 93, 2009, 2400, 2505
BmiI GGNNCC 7 cut(s) 94, 378, 502, 522, 1323, 1512, 1591
BmrFI CCNGG 3 cut(s) 110, 635, 1533
BmrI ACTGGG 1 cut(s) 1393
BmsI GCATC 8 cut(s) 532, 894, 1442, 1894, 2056, 2203, 2509, 2531
BmuI ACTGGG 1 cut(s) 1393
BplI GAGNNNNNCTC 4 cut(s) 80, 112, 1581, 1613
BpmI CTGGAG 2 cut(s) 2351, 2358
Bpu10I CCTNAGC 1 cut(s) 2169
Bpu1102I GCTNAGC 1 cut(s) 2556
BpuEI CTTGAG 3 cut(s) 526, 1123, 1232
BpuMI CCSGG 3 cut(s) 110, 635, 1533
BsaAI YACGTR 1 cut(s) 2224
BsaBI GATNNNNATC 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 1511
BsaJI CCNNGG 5 cut(s) 299, 415, 708, 1771, 1795
BsaWI WCCGGW 3 cut(s) 1175, 1324, 1436
Bsc4I CCNNNNNNNGG 4 cut(s) 300, 371, 1124, 1858
Bse118I RCCGGY 1 cut(s) 1175
Bse1I ACTGG 6 cut(s) 158, 323, 583, 1155, 1399, 1703
Bse3DI GCAATG 2 cut(s) 1480, 1686
Bse8I GATNNNNATC 1 cut(s) 546
BseAI TCCGGA 2 cut(s) 1324, 1436
BseDI CCNNGG 5 cut(s) 299, 415, 708, 1771, 1795
BseGI GGATG 6 cut(s) 17, 547, 682, 1016, 1433, 2446
BseJI GATNNNNATC 1 cut(s) 546
BseLI CCNNNNNNNGG 4 cut(s) 300, 371, 1124, 1858
BseMI GCAATG 2 cut(s) 1480, 1686
BseNI ACTGG 6 cut(s) 158, 323, 583, 1155, 1399, 1703
BseRI GAGGAG 2 cut(s) 943, 1602
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 4 cut(s) 1751, 1963, 2056, 2498
BseYI CCCAGC 2 cut(s) 740, 2037
BshFI GGCC 6 cut(s) 322, 406, 1770, 2187, 2209, 2402
BshNI GGYRCC 1 cut(s) 1510
BshTI ACCGGT 1 cut(s) 1175
BsiHKAI GWGCWC 3 cut(s) 196, 835, 2337
BsiHKCI CYCGRG 1 cut(s) 2601
BsiSI CCGG 6 cut(s) 110, 635, 1176, 1325, 1437, 1533
BslFI GGGAC 2 cut(s) 92, 106
BslI CCNNNNNNNGG 4 cut(s) 300, 371, 1124, 1858
BsmAI GTCTC 3 cut(s) 346, 1899, 2598
BsmBI CGTCTC 1 cut(s) 2598
BsmFI GGGAC 2 cut(s) 92, 106
BsmI GAATGC 2 cut(s) 2170, 2577
BsnI GGCC 6 cut(s) 322, 406, 1770, 2187, 2209, 2402
BsoBI CYCGRG 1 cut(s) 2601
Bsp1286I GDGCHC 4 cut(s) 196, 379, 835, 2337
Bsp13I TCCGGA 2 cut(s) 1324, 1436
Bsp1407I TGTACA 1 cut(s) 204
Bsp1720I GCTNAGC 1 cut(s) 2556
BspACI CCGC 2 cut(s) 654, 2206
BspANI GGCC 6 cut(s) 322, 406, 1770, 2187, 2209, 2402
BspEI TCCGGA 2 cut(s) 1324, 1436
BspHI TCATGA 1 cut(s) 1939
BspLI GGNNCC 7 cut(s) 94, 378, 502, 522, 1323, 1512, 1591
BspMI ACCTGC 1 cut(s) 2411
BspPI GGATC 9 cut(s) 290, 495, 508, 515, 528, 1053, 1146, 1356, 2255
BspT107I GGYRCC 1 cut(s) 1510
BspTI CTTAAG 1 cut(s) 878
BsrDI GCAATG 2 cut(s) 1480, 1686
BsrFI RCCGGY 1 cut(s) 1175
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 6 cut(s) 158, 323, 583, 1155, 1399, 1703
BssAI RCCGGY 1 cut(s) 1175
BssECI CCNNGG 5 cut(s) 299, 415, 708, 1771, 1795
BssNI GRCGYC 1 cut(s) 1511
BssSI CACGAG 1 cut(s) 1546
BssT1I CCWWGG 4 cut(s) 299, 415, 708, 1795
Bst2BI CACGAG 1 cut(s) 1546
Bst4CI ACNGT 7 cut(s) 146, 340, 811, 964, 990, 1556, 2233
Bst6I CTCTTC 3 cut(s) 303, 1504, 2341
BstACI GRCGYC 1 cut(s) 1511
BstAFI CTTAAG 1 cut(s) 878
BstAPI GCANNNNNTGC 1 cut(s) 2203
BstAUI TGTACA 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 2224
BstC8I GCNNGC 1 cut(s) 2251
BstDSI CCRYGG 1 cut(s) 1771
BstEII GGTNACC 2 cut(s) 1019, 1072
BstF5I GGATG 6 cut(s) 17, 547, 682, 1016, 1433, 2446
BstH2I RGCGCY 1 cut(s) 1514
BstHHI GCGC 1 cut(s) 1513
BstMAI GTCTC 3 cut(s) 346, 1899, 2598
BstMWI GCNNNNNNNGC 6 cut(s) 1275, 1598, 2043, 2066, 2203, 2495
BstNSI RCATGY 1 cut(s) 1473
BstPI GGTNACC 2 cut(s) 1019, 1072
BstSCI CCNGG 3 cut(s) 108, 633, 1531
BstSFI CTRYAG 4 cut(s) 276, 1184, 2308, 2572
BstSLI GKGCMC 1 cut(s) 835
BstV1I GCAGC 4 cut(s) 1751, 1963, 2056, 2498
BstX2I RGATCY 5 cut(s) 280, 500, 520, 1138, 1348
BstXI CCANNNNNNTGG 1 cut(s) 2006
BstYI RGATCY 5 cut(s) 280, 500, 520, 1138, 1348
BsuI GTATCC 1 cut(s) 522
BsuRI GGCC 6 cut(s) 322, 406, 1770, 2187, 2209, 2402
BtgI CCRYGG 1 cut(s) 1771
BtgZI GCGATG 2 cut(s) 1152, 2316
BtrI CACGTC 1 cut(s) 1208
BtsCI GGATG 6 cut(s) 17, 547, 682, 1016, 1433, 2446
BtsIMutI CAGTG 5 cut(s) 165, 458, 1148, 2030, 2229
BveI ACCTGC 1 cut(s) 2411
Cac8I GCNNGC 1 cut(s) 2251
CaiI CAGNNNCTG 1 cut(s) 1192
CciI TCATGA 1 cut(s) 1939
CfoI GCGC 1 cut(s) 1513
Cfr10I RCCGGY 1 cut(s) 1175
Cfr13I GGNCC 4 cut(s) 93, 2009, 2400, 2505
Csp6I GTAC 2 cut(s) 205, 273
CspAI ACCGGT 1 cut(s) 1175
CviAII CATG 7 cut(s) 30, 436, 569, 1069, 1470, 1940, 2476
CviQI GTAC 2 cut(s) 205, 273
DinI GGCGCC 1 cut(s) 1512
EaeI YGGCCR 2 cut(s) 320, 2207
Eam1104I CTCTTC 3 cut(s) 303, 1504, 2341
EarI CTCTTC 3 cut(s) 303, 1504, 2341
Ecl136II GAGCTC 2 cut(s) 194, 2335
Eco130I CCWWGG 4 cut(s) 299, 415, 708, 1795
Eco24I GRGCYC 3 cut(s) 196, 379, 2337
Eco32I GATATC 2 cut(s) 643, 1660
Eco47I GGWCC 3 cut(s) 93, 2009, 2505
Eco53kI GAGCTC 2 cut(s) 194, 2335
Eco57I CTGAAG 1 cut(s) 959
Eco72I CACGTG 1 cut(s) 2224
Eco88I CYCGRG 1 cut(s) 2601
Eco91I GGTNACC 2 cut(s) 1019, 1072
EcoICRI GAGCTC 2 cut(s) 194, 2335
EcoO109I RGGNCCY 1 cut(s) 93
EcoO65I GGTNACC 2 cut(s) 1019, 1072
EcoRI GAATTC 3 cut(s) 47, 1702, 1899
EcoRV GATATC 2 cut(s) 643, 1660
EcoT14I CCWWGG 4 cut(s) 299, 415, 708, 1795
EcoT22I ATGCAT 1 cut(s) 2524
EcoT38I GRGCYC 3 cut(s) 196, 379, 2337
EgeI GGCGCC 1 cut(s) 1512
EheI GGCGCC 1 cut(s) 1512
ErhI CCWWGG 4 cut(s) 299, 415, 708, 1795
Esp3I CGTCTC 1 cut(s) 2598
FaeI CATG 7 cut(s) 33, 439, 572, 1072, 1473, 1943, 2479
FalI AAGNNNNNCTT 2 cut(s) 1377, 1409
FaqI GGGAC 2 cut(s) 92, 106
FatI CATG 7 cut(s) 29, 435, 568, 1068, 1469, 1939, 2475
FauNDI CATATG 1 cut(s) 526
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 5 cut(s) 1740, 1952, 2070, 2207, 2487
FokI GGATG 6 cut(s) 4, 554, 689, 1023, 1420, 2453
FriOI GRGCYC 3 cut(s) 196, 379, 2337
Fsp4HI GCNGC 5 cut(s) 1740, 1952, 2070, 2207, 2487
FspBI CTAG 2 cut(s) 2450, 2492
GlaI GCGC 1 cut(s) 1512
GluI GCNGC 5 cut(s) 1740, 1952, 2070, 2207, 2487
GsaI CCCAGC 2 cut(s) 744, 2041
GsuI CTGGAG 2 cut(s) 2351, 2358
HaeII RGCGCY 1 cut(s) 1514
HaeIII GGCC 6 cut(s) 322, 406, 1770, 2187, 2209, 2402
HapII CCGG 6 cut(s) 110, 635, 1176, 1325, 1437, 1533
HhaI GCGC 1 cut(s) 1513
Hin1I GRCGYC 1 cut(s) 1511
Hin1II CATG 7 cut(s) 33, 439, 572, 1072, 1473, 1943, 2479
Hin6I GCGC 1 cut(s) 1511
HinP1I GCGC 1 cut(s) 1511
HincII GTYRAC 2 cut(s) 576, 1242
HindII GTYRAC 2 cut(s) 576, 1242
HindIII AAGCTT 3 cut(s) 553, 1085, 1417
HpaI GTTAAC 1 cut(s) 1242
HpaII CCGG 6 cut(s) 110, 635, 1176, 1325, 1437, 1533
HphI GGTGA 4 cut(s) 797, 1031, 1139, 1190
Hpy166II GTNNAC 7 cut(s) 205, 576, 833, 1181, 1242, 1792, 1966
Hpy188I TCNGA 8 cut(s) 191, 287, 823, 1035, 1093, 1227, 1492, 2318
Hpy8I GTNNAC 7 cut(s) 205, 576, 833, 1181, 1242, 1792, 1966
HpyAV CCTTC 7 cut(s) 246, 656, 771, 983, 1055, 1289, 1922
HpyCH4III ACNGT 7 cut(s) 146, 340, 811, 964, 990, 1556, 2233
HpyCH4IV ACGT 3 cut(s) 1207, 2223, 2607
HpyF10VI GCNNNNNNNGC 6 cut(s) 1275, 1598, 2043, 2066, 2203, 2495
HpySE526I ACGT 3 cut(s) 1207, 2223, 2607
Hsp92I GRCGYC 1 cut(s) 1511
Hsp92II CATG 7 cut(s) 33, 439, 572, 1072, 1473, 1943, 2479
HspAI GCGC 1 cut(s) 1511
KasI GGCGCC 1 cut(s) 1510
Kpn2I TCCGGA 2 cut(s) 1324, 1436
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 1242
LmnI GCTCC 4 cut(s) 382, 419, 1589, 2332
Lsp1109I GCAGC 4 cut(s) 1751, 1963, 2056, 2498
LweI GCATC 8 cut(s) 532, 894, 1442, 1894, 2056, 2203, 2509, 2531
MaeI CTAG 2 cut(s) 2450, 2492
MaeII ACGT 3 cut(s) 1207, 2223, 2607
MfeI CAATTG 3 cut(s) 393, 912, 993
MflI RGATCY 5 cut(s) 280, 500, 520, 1138, 1348
MhlI GDGCHC 4 cut(s) 196, 379, 835, 2337
MlsI TGGCCA 1 cut(s) 322
MluNI TGGCCA 1 cut(s) 322
Mly113I GGCGCC 1 cut(s) 1511
MlyI GAGTC 3 cut(s) 583, 2368, 2372
MmeI TCCRAC 4 cut(s) 498, 1009, 1058, 1242
Mox20I TGGCCA 1 cut(s) 322
Mph1103I ATGCAT 1 cut(s) 2524
MroI TCCGGA 2 cut(s) 1324, 1436
MscI TGGCCA 1 cut(s) 322
MseI TTAA 9 cut(s) 585, 597, 620, 780, 879, 1143, 1241, 1734, 2385
MslI CAYNNNNRTG 2 cut(s) 1667, 2004
Msp20I TGGCCA 1 cut(s) 322
MspCI CTTAAG 1 cut(s) 878
MspI CCGG 6 cut(s) 110, 635, 1176, 1325, 1437, 1533
MspR9I CCNGG 3 cut(s) 110, 635, 1533
MunI CAATTG 3 cut(s) 393, 912, 993
Mva1269I GAATGC 2 cut(s) 2170, 2577
MwoI GCNNNNNNNGC 6 cut(s) 1275, 1598, 2043, 2066, 2203, 2495
NarI GGCGCC 1 cut(s) 1511
NciI CCSGG 3 cut(s) 110, 635, 1533
NdeI CATATG 1 cut(s) 526
NlaIII CATG 7 cut(s) 33, 439, 572, 1072, 1473, 1943, 2479
NlaIV GGNNCC 7 cut(s) 94, 378, 502, 522, 1323, 1512, 1591
NmuCI GTSAC 5 cut(s) 456, 1019, 1127, 2224, 2360
NsiI ATGCAT 1 cut(s) 2524
NspI RCATGY 1 cut(s) 1473
PaeR7I CTCGAG 1 cut(s) 2601
PagI TCATGA 1 cut(s) 1939
PaqCI CACCTGC 1 cut(s) 2411
PctI GAATGC 2 cut(s) 2170, 2577
PflFI GACNNNGTC 1 cut(s) 338
PflMI CCANNNNNTGG 1 cut(s) 1124
PfoI TCCNGGA 1 cut(s) 1531
PinAI ACCGGT 1 cut(s) 1175
PkrI GCNGC 5 cut(s) 1741, 1953, 2071, 2208, 2488
PleI GAGTC 3 cut(s) 583, 2367, 2372
PluTI GGCGCC 1 cut(s) 1514
PmaCI CACGTG 1 cut(s) 2224
PmlI CACGTG 1 cut(s) 2224
PpsI GAGTC 3 cut(s) 583, 2367, 2372
Ppu21I YACGTR 1 cut(s) 2224
PpuMI RGGWCCY 1 cut(s) 93
PshBI ATTAAT 1 cut(s) 780
Psp124BI GAGCTC 2 cut(s) 196, 2337
Psp5II RGGWCCY 1 cut(s) 93
PspCI CACGTG 1 cut(s) 2224
PspEI GGTNACC 2 cut(s) 1019, 1072
PspFI CCCAGC 2 cut(s) 740, 2037
PspN4I GGNNCC 7 cut(s) 94, 378, 502, 522, 1323, 1512, 1591
PspPI GGNCC 4 cut(s) 93, 2009, 2400, 2505
PspPPI RGGWCCY 1 cut(s) 93
PstNI CAGNNNCTG 1 cut(s) 1192
PsuI RGATCY 5 cut(s) 280, 500, 520, 1138, 1348
PsyI GACNNNGTC 1 cut(s) 338
RsaI GTAC 2 cut(s) 206, 274
RsaNI GTAC 2 cut(s) 205, 273
RseI CAYNNNNRTG 2 cut(s) 1667, 2004
SacI GAGCTC 2 cut(s) 196, 2337
SaqAI TTAA 9 cut(s) 585, 597, 620, 780, 879, 1143, 1241, 1734, 2385
SatI GCNGC 5 cut(s) 1740, 1952, 2070, 2207, 2487
Sau96I GGNCC 4 cut(s) 93, 2009, 2400, 2505
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 3 cut(s) 583, 2368, 2372
ScrFI CCNGG 3 cut(s) 110, 635, 1533
SduI GDGCHC 4 cut(s) 196, 379, 835, 2337
SfaNI GCATC 8 cut(s) 532, 894, 1442, 1894, 2056, 2203, 2509, 2531
SfcI CTRYAG 4 cut(s) 276, 1184, 2308, 2572
SfoI GGCGCC 1 cut(s) 1512
Sfr274I CTCGAG 1 cut(s) 2601
SinI GGWCC 3 cut(s) 93, 2009, 2505
SlaI CTCGAG 1 cut(s) 2601
SmiMI CAYNNNNRTG 2 cut(s) 1667, 2004
SmlI CTYRAG 5 cut(s) 505, 878, 1102, 1211, 2601
SmoI CTYRAG 5 cut(s) 505, 878, 1102, 1211, 2601
SsiI CCGC 2 cut(s) 654, 2206
SspDI GGCGCC 1 cut(s) 1510
SspI AATATT 1 cut(s) 2270
SspMI CTAG 2 cut(s) 2450, 2492
SstI GAGCTC 2 cut(s) 196, 2337
StyD4I CCNGG 3 cut(s) 108, 633, 1531
StyI CCWWGG 4 cut(s) 299, 415, 708, 1795
TaaI ACNGT 7 cut(s) 146, 340, 811, 964, 990, 1556, 2233
TaiI ACGT 3 cut(s) 1210, 2226, 2610
TaqI TCGA 4 cut(s) 475, 768, 1897, 2602
TatI WGTACW 2 cut(s) 204, 272
TauI GCSGC 1 cut(s) 2209
Tru1I TTAA 9 cut(s) 585, 597, 620, 780, 879, 1143, 1241, 1734, 2385
Tru9I TTAA 9 cut(s) 585, 597, 620, 780, 879, 1143, 1241, 1734, 2385
TscAI CASTG 5 cut(s) 165, 465, 1155, 2037, 2236
TseFI GTSAC 5 cut(s) 456, 1019, 1127, 2224, 2360
TseI GCWGC 4 cut(s) 1739, 1951, 2069, 2486
Tsp45I GTSAC 5 cut(s) 456, 1019, 1127, 2224, 2360
TspGWI ACGGA 3 cut(s) 504, 685, 2497
TspRI CASTG 5 cut(s) 165, 465, 1155, 2037, 2236
Tth111I GACNNNGTC 1 cut(s) 338
Van91I CCANNNNNTGG 1 cut(s) 1124
Vha464I CTTAAG 1 cut(s) 878
VneI GTGCAC 1 cut(s) 831
VpaK11BI GGWCC 3 cut(s) 93, 2009, 2505
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 5 cut(s) 47, 927, 1284, 1702, 1899
XceI RCATGY 1 cut(s) 1473
XcmI CCANNNNNNNNNTGG 2 cut(s) 992, 1816
XhoI CTCGAG 1 cut(s) 2601
XspI CTAG 2 cut(s) 2450, 2492
ZrmI AGTACT 1 cut(s) 274
Zsp2I ATGCAT 1 cut(s) 2524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.