MD10G1229500.v1.1

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016189
Physical Location & Seq
Reverse (-)
32645091 .. 32647893
2803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1229500.v1.1.491

Sequence Viewer

Length: 888 bp
ATGGAGAGAGAGGGAAGGGTGGAGGTGGAAAAAGTGGGAGGAAGGTCAACAGTGACAAGATGCTTCTCAAAGTATCCTCTCAAGTTCATCATCCCCAGAAAGGTGGGTCCCTCCAAAACTGATGCTGTTTGGGTTTACAACCTCACCTATGGTGGTGGTATTGTTTCTGGAGATTCTATTTCATGTGAGTTTAACATTGGAGATGGCTGCACCACTGTCTTAACAACCCAAGCTTCCACTAAGGTTTACAAGTCTGTGGGATTCAAGTGCTGTGAACAAGTCTTGGAGGCAAGGGTTGGGAGCAATGCCCTTTTGGCGGTCATTCCTGATCCTGTGACATGTTTTTCCACGGCAAGATACTCTCAGAAACAAGTCTTTAGGGTAGTTTCCGACTCAAGTTTGGTCATTGTGGATTGGATTACCAGTGGGCGTCACGAAAGTGGAGAAAAATGGGATTTCGATCTTTACAAGAGCATCAACCACATATTTATGGAAAATGATGAACCTCTGTTTCTGGACGCGGTGCACCTCGAGCGTGGAAATATCTCTTCAATTGCTGAACGAATGCAGGACTACCAAGTAATTGCAATGGTTGTACTCTTGGGGCCAAAGATCAAGCACATTCAGAACCTAGTTCAAGAAAACGTGAAGAGGATCATGTCTGACCAATTACACATTCCTTCCACCGCGTCAGGTCACCAAATAAAAACAAATTCTGATAATCGCTTCGCCAAACCAAGCTTTATTGCTTCTTCAAGTGTTTTTGGTCCTAAGGGAATCGGGGTGGTTGTTCGTGTAGCTGCTACAACAACTGAATCAGTTTATAAGTTCCTGCAGCATCAATTGGCCGGCATGGAGCCACTACTTGGGGTATCGCCATATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0051336 GO:0051345 GO:0065007 GO:0065009 GO:1905181 GO:1905182
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UreD

Protein Analysis

296

Amino Acids

32.61

Weight (kDa)

8.45

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UreD PF01774 46 - 272 2e-54 UreD urease accessory protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35035 AT2G35035
fragaria_vesca FvH4_3g12650
malus_domestica MD10G1229500.v1.1
prunus_persica Prupe.4G115300_v2.0.a1
pyrus_communis pycom10g19300
rosa_chinensis RchiOBHm_Chr5g0020621
rosa_laevigata RLG00000032544
rosa_multiflora Rmu_sc0005607.1_g000014
rosa_roxburghii Rroxscaffold_1G00057670
rosa_rugosa Rorug05G0061900 Rorug05G0062000
rosa_samantha Rh5AG151700 Rh5BG151300
rosa_wichuraiana Rw5G013440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 825
AccB7I CCANNNNNTGG 1 cut(s) 866
AccII CGCG 2 cut(s) 521, 689
AciI CCGC 3 cut(s) 317, 521, 687
AclWI GGATC 2 cut(s) 323, 662
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 1 cut(s) 712
AcyI GRCGYC 1 cut(s) 430
AfaI GTAC 1 cut(s) 597
AfiI CCNNNNNNNGG 3 cut(s) 100, 316, 866
AflIII ACRYGT 1 cut(s) 338
AgsI TTSAA 4 cut(s) 265, 552, 638, 756
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AleI CACNNNNGTG 1 cut(s) 438
AluBI AGCT 3 cut(s) 233, 741, 800
AluI AGCT 3 cut(s) 233, 741, 800
Alw21I GWGCWC 1 cut(s) 528
Alw44I GTGCAC 1 cut(s) 524
AlwI GGATC 2 cut(s) 323, 662
Ama87I CYCGRG 1 cut(s) 530
AoxI GGCC 2 cut(s) 605, 846
ApaLI GTGCAC 1 cut(s) 524
ApeKI GCWGC 3 cut(s) 207, 800, 835
ApoI RAATTY 1 cut(s) 712
AspS9I GGNCC 3 cut(s) 107, 605, 767
AsuHPI GGTGA 2 cut(s) 136, 689
AvaI CYCGRG 1 cut(s) 530
AvaII GGWCC 2 cut(s) 107, 767
AxyI CCTNAGG 1 cut(s) 771
BaeGI GKGCMC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 194, 787, 847
BccI CCATC 1 cut(s) 197
BceAI ACGGC 1 cut(s) 366
BciVI GTATCC 1 cut(s) 84
BfaI CTAG 1 cut(s) 632
BfmI CTRYAG 1 cut(s) 833
BfuI GTATCC 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 314
BisI GCNGC 3 cut(s) 208, 801, 836
BlsI GCNGC 3 cut(s) 209, 802, 837
Bme18I GGWCC 2 cut(s) 107, 767
BmeT110I CYCGRG 1 cut(s) 530
BmgT120I GGNCC 3 cut(s) 107, 605, 767
BmiI GGNNCC 4 cut(s) 108, 109, 606, 858
BmsI GCATC 4 cut(s) 50, 112, 483, 847
BpmI CTGGAG 1 cut(s) 189
BpuEI CTTGAG 2 cut(s) 65, 379
BsaBI GATNNNNATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 430
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 316, 866
Bse118I RCCGGY 1 cut(s) 848
Bse1I ACTGG 1 cut(s) 423
Bse21I CCTNAGG 1 cut(s) 771
Bse3DI GCAATG 2 cut(s) 310, 594
Bse8I GATNNNNATC 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 1 cut(s) 90
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 3 cut(s) 100, 316, 866
BseMI GCAATG 2 cut(s) 310, 594
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 423
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 3 cut(s) 194, 787, 847
BsgI GTGCAG 1 cut(s) 193
Bsh1236I CGCG 2 cut(s) 521, 689
BshFI GGCC 2 cut(s) 607, 848
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 1 cut(s) 530
BsiSI CCGG 1 cut(s) 849
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 100, 316, 866
BsmFI GGGAC 1 cut(s) 93
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 2 cut(s) 607, 848
BsoBI CYCGRG 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 4 cut(s) 328, 460, 612, 654
BspACI CCGC 3 cut(s) 317, 521, 687
BspANI GGCC 2 cut(s) 607, 848
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 2 cut(s) 521, 689
BspLI GGNNCC 4 cut(s) 108, 109, 606, 858
BspMAI CTGCAG 1 cut(s) 837
BspPI GGATC 2 cut(s) 323, 662
BsrDI GCAATG 2 cut(s) 310, 594
BsrFI RCCGGY 1 cut(s) 848
BsrI ACTGG 1 cut(s) 423
BssAI RCCGGY 1 cut(s) 848
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 4 cut(s) 328, 460, 612, 654
BssNI GRCGYC 1 cut(s) 430
Bst4CI ACNGT 2 cut(s) 52, 217
Bst6I CTCTTC 2 cut(s) 553, 644
BstACI GRCGYC 1 cut(s) 430
BstC8I GCNNGC 1 cut(s) 850
BstDEI CTNAG 3 cut(s) 240, 363, 771
BstDSI CCRYGG 1 cut(s) 348
BstEII GGTNACC 1 cut(s) 695
BstF5I GGATG 1 cut(s) 90
BstFNI CGCG 2 cut(s) 521, 689
BstKTI GATC 4 cut(s) 331, 463, 615, 657
BstMBI GATC 4 cut(s) 328, 460, 612, 654
BstMWI GCNNNNNNNGC 2 cut(s) 314, 532
BstNSI RCATGY 1 cut(s) 342
BstPI GGTNACC 1 cut(s) 695
BstSFI CTRYAG 1 cut(s) 833
BstSLI GKGCMC 1 cut(s) 528
BstUI CGCG 2 cut(s) 521, 689
BstV1I GCAGC 3 cut(s) 194, 787, 847
BstXI CCANNNNNNTGG 1 cut(s) 103
Bsu36I CCTNAGG 1 cut(s) 771
BsuI GTATCC 1 cut(s) 84
BsuRI GGCC 2 cut(s) 607, 848
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 1 cut(s) 90
BtsIMutI CAGTG 4 cut(s) 57, 213, 430, 883
Cac8I GCNNGC 1 cut(s) 850
Cfr10I RCCGGY 1 cut(s) 848
Cfr13I GGNCC 3 cut(s) 107, 605, 767
CseI GACGC 3 cut(s) 419, 527, 678
Csp6I GTAC 1 cut(s) 596
CviAII CATG 4 cut(s) 183, 339, 658, 853
CviJI RGCY 7 cut(s) 207, 233, 607, 741, 800, 848, 859
CviKI_1 RGCY 7 cut(s) 207, 233, 607, 741, 800, 848, 859
CviQI GTAC 1 cut(s) 596
DdeI CTNAG 3 cut(s) 240, 363, 771
DpnI GATC 4 cut(s) 330, 462, 614, 656
DpnII GATC 4 cut(s) 328, 460, 612, 654
EaeI YGGCCR 1 cut(s) 846
Eam1104I CTCTTC 2 cut(s) 553, 644
EarI CTCTTC 2 cut(s) 553, 644
Eco47I GGWCC 2 cut(s) 107, 767
Eco81I CCTNAGG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 530
Eco91I GGTNACC 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 695
FaeI CATG 4 cut(s) 186, 342, 661, 856
FaiI YATR 9 cut(s) 150, 184, 340, 485, 491, 659, 825, 854, 880
FaqI GGGAC 1 cut(s) 93
FatI CATG 4 cut(s) 182, 338, 657, 852
Fnu4HI GCNGC 3 cut(s) 208, 801, 836
FokI GGATG 1 cut(s) 77
Fsp4HI GCNGC 3 cut(s) 208, 801, 836
FspBI CTAG 1 cut(s) 632
GluI GCNGC 3 cut(s) 208, 801, 836
GsuI CTGGAG 1 cut(s) 189
HaeIII GGCC 2 cut(s) 607, 848
HapII CCGG 1 cut(s) 849
HgaI GACGC 3 cut(s) 419, 527, 678
Hin1I GRCGYC 1 cut(s) 430
Hin1II CATG 4 cut(s) 186, 342, 661, 856
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HindIII AAGCTT 2 cut(s) 231, 739
HinfI GANTC 5 cut(s) 173, 261, 392, 777, 815
HpaII CCGG 1 cut(s) 849
HphI GGTGA 2 cut(s) 136, 689
Hpy166II GTNNAC 5 cut(s) 48, 136, 247, 275, 526
Hpy188I TCNGA 5 cut(s) 366, 391, 627, 664, 718
Hpy188III TCNNGA 5 cut(s) 168, 326, 434, 515, 638
Hpy8I GTNNAC 5 cut(s) 48, 136, 247, 275, 526
HpyAV CCTTC 3 cut(s) 9, 36, 690
HpyCH4III ACNGT 2 cut(s) 52, 217
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 5 cut(s) 210, 526, 568, 587, 835
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 532
HpyF3I CTNAG 3 cut(s) 240, 363, 771
HpySE526I ACGT 1 cut(s) 645
Hsp92I GRCGYC 1 cut(s) 430
Hsp92II CATG 4 cut(s) 186, 342, 661, 856
KflI GGGWCCC 1 cut(s) 107
KroI GCCGGC 1 cut(s) 848
KroNI GCCGGC 1 cut(s) 850
Kzo9I GATC 4 cut(s) 328, 460, 612, 654
LmnI GCTCC 2 cut(s) 300, 856
Lsp1109I GCAGC 3 cut(s) 194, 787, 847
LweI GCATC 4 cut(s) 50, 112, 483, 847
MaeI CTAG 1 cut(s) 632
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 4 cut(s) 52, 334, 431, 695
MalI GATC 4 cut(s) 330, 462, 614, 656
MboI GATC 4 cut(s) 328, 460, 612, 654
MboII GAAGA 3 cut(s) 540, 661, 744
MfeI CAATTG 2 cut(s) 552, 842
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 5 cut(s) 552, 582, 668, 712, 842
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 1 cut(s) 414
MroNI GCCGGC 1 cut(s) 848
MseI TTAA 2 cut(s) 192, 221
MslI CAYNNNNRTG 2 cut(s) 438, 488
MspI CCGG 1 cut(s) 849
MunI CAATTG 2 cut(s) 552, 842
Mva1269I GAATGC 1 cut(s) 570
MvnI CGCG 2 cut(s) 521, 689
MwoI GCNNNNNNNGC 2 cut(s) 314, 532
NaeI GCCGGC 1 cut(s) 850
NdeII GATC 4 cut(s) 328, 460, 612, 654
NgoMIV GCCGGC 1 cut(s) 848
NlaIII CATG 4 cut(s) 186, 342, 661, 856
NlaIV GGNNCC 4 cut(s) 108, 109, 606, 858
NmuCI GTSAC 4 cut(s) 52, 334, 431, 695
NspI RCATGY 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 438
PaeR7I CTCGAG 1 cut(s) 530
PciI ACATGT 1 cut(s) 338
PctI GAATGC 1 cut(s) 570
PdiI GCCGGC 1 cut(s) 850
PfeI GAWTC 4 cut(s) 173, 261, 777, 815
PflMI CCANNNNNTGG 1 cut(s) 866
PkrI GCNGC 3 cut(s) 209, 802, 837
PleI GAGTC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 386
PpuMI RGGWCCY 1 cut(s) 107
PscI ACATGT 1 cut(s) 338
PsiI TTATAA 1 cut(s) 825
Psp5II RGGWCCY 1 cut(s) 107
PspEI GGTNACC 1 cut(s) 695
PspN4I GGNNCC 4 cut(s) 108, 109, 606, 858
PspPI GGNCC 3 cut(s) 107, 605, 767
PspPPI RGGWCCY 1 cut(s) 107
PspXI VCTCGAGB 1 cut(s) 530
PstI CTGCAG 1 cut(s) 837
RsaI GTAC 1 cut(s) 597
RsaNI GTAC 1 cut(s) 596
RseI CAYNNNNRTG 2 cut(s) 438, 488
SaqAI TTAA 2 cut(s) 192, 221
SatI GCNGC 3 cut(s) 208, 801, 836
Sau3AI GATC 4 cut(s) 328, 460, 612, 654
Sau96I GGNCC 3 cut(s) 107, 605, 767
SchI GAGTC 1 cut(s) 386
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 4 cut(s) 50, 112, 483, 847
SfcI CTRYAG 1 cut(s) 833
Sfr274I CTCGAG 1 cut(s) 530
SinI GGWCC 2 cut(s) 107, 767
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 2 cut(s) 438, 488
SmlI CTYRAG 3 cut(s) 80, 394, 530
SmoI CTYRAG 3 cut(s) 80, 394, 530
Sse9I AATT 5 cut(s) 552, 582, 668, 712, 842
SsiI CCGC 3 cut(s) 317, 521, 687
SspMI CTAG 1 cut(s) 632
TaaI ACNGT 2 cut(s) 52, 217
TaiI ACGT 1 cut(s) 648
TaqI TCGA 2 cut(s) 459, 531
TasI AATT 5 cut(s) 552, 582, 668, 712, 842
TatI WGTACW 1 cut(s) 595
TfiI GAWTC 4 cut(s) 173, 261, 777, 815
Tru1I TTAA 2 cut(s) 192, 221
Tru9I TTAA 2 cut(s) 192, 221
TscAI CASTG 3 cut(s) 57, 220, 430
TseFI GTSAC 4 cut(s) 52, 334, 431, 695
TseI GCWGC 3 cut(s) 207, 800, 835
Tsp45I GTSAC 4 cut(s) 52, 334, 431, 695
TspDTI ATGAA 3 cut(s) 76, 171, 516
TspRI CASTG 3 cut(s) 57, 220, 430
Van91I CCANNNNNTGG 1 cut(s) 866
VneI GTGCAC 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 107, 767
XapI RAATTY 1 cut(s) 712
XceI RCATGY 1 cut(s) 342
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.