Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0014923

Physical Location & Seq

Genomic Coordinates Forward (+)

34600339 .. 34606877

Gene Structure 6539 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1253500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2556 bp

ATGGCGCTGGTAATGCACCGAGACTCGCCGGGAAGCAGCAGCGGCGGGAGGCAGCAGATGGATTCGAGCAAGTACGTTCGGTACACGCCGGAGCAGGTGGAGGCGTTGGAGAGAGTCTACTCAGAGTGCCCAAAGCCCAGCTCCCTGAGAAGGCAGCAGCTCATCAGAGAGTGCCCCATTCTCTCCAACATTGAGCCTAAACAGATCAAAGTCTGGTTCCAGAACCGCAGATGCCGAGAGAAGCAGCGGAAGGAATCTTCTCGACTCCAGACAGTGAACAGAAAGCTGACTGCGATGAACAAGCTGTTAATGGAAGAAAATGACCGCTTACAGAAGCAAGTTTCTCATTTGGTTTATGAGAATGGATTTATGAAGCAGCAACTGCATACCGCATCAGGAACGACCACAGACAATAGCTGCGAGTCTGTGGTCATGAGTGGTCAGCACCAACAACAGCAAAACCCAACTCCCCAGCACCCCCAAAGGGATGCTAACAACCCAGCTGGTCTTCTTGCAATAGCAGAAGAGACCTTGGCAGAGTTCCTTTCTAAGGCTACTGGAACTGCTGTCGACTGGGTCCAGATGATTGGGATGAAGCCTGGTCCGGATTCTATTGGCATCGTTGCTGTCTCCCGCAACTGCAGTGGAGTAGCAGCACGAGCTTGCGGTCTTGTGAGCCTAGAGCCCACAAAGGTCGCCGAAATACTCAAAGATCGTAAATCTTGGTTTCGCGATTGCCGATGCCTTGAGGTATTAAGTCTAATCCCTGCGGGGAATGGTGGAACAATTGAGCTCGTATACATGCAGACTTATGCACCCACAACATTGGCTGCGGCACGTGACTTTTGGACACTAAGATATACTACAAGTTTGGAAGACGGCAGTCTTGTGGTATGCGAGAGGTCATTGACTTCCTCTACTGGTGGCCCACCTGGGCCAACATCTGCAAGTTTTGTCAGAGCTGAAATGCTTCCTAGTGGTTATCTTATCCGACCTTGTGAGGGTGGAGGTTCCATTATCAACATTGTGGATCATGTTGATTTAGACGCTTGGAGCGTTCCTGAAGTTCTCAGGTCACTTTATGAATCCTCCAAAATCCTTGCTCAGAAGACGACCATTTCTGCCCTGCGACACATAAGACAGATAGCTCAAGAGTCTAGTGGAGAAATTCAGCATGGTGGAGGTCGCCAACCTGCTGTTCTGAGGACATTTTGTCAGAGGCTGTGTAGTGGGTTTAACGATGCTGTTAATGGGTTTGCGGATGACGGTTGGACACTTATGGGTAGTGATGGTGTGGAAGATGTTACCATTGCTATTAACTCATCTCCGAAAGTGCTTGGTTCCCAGTATAACACTTCAATTTTCCCACCTTTTGGAGGAGGGGTGCTGTGTGCCAAGGCATCAATGCTGCTGCAGAGTGTTCCCCCTGCTTTGCTTGTTCGTTTCCTGAGGGAGCACCGGGCTGAGTGGGCTGACTATGGGGTTGATGCATACTCTGCTGCATGTCTTAAAGCTAGTCCATATGCAGTTCCTCTTGCAAGAGGTGGTGGCTTTCCGAGCACCCAGAATATTTTACCGCTTGCACAAACTGTGGAGCATGAGGAGTTTTTAGAGGTAGTTCGGCTAGAGGGTCCTGCTTTCTCTCCTGAAGATGTTGCTTTGACACGGGATATGTACTTATTACAGTTGTGCAGTGGAGTTGATGAGAATGCAGTTGGTGCCTGCGCTCAGCTTGTCTTTGCACCTATTGATGAATCATTTGCTGATGATGCTCCTCTGTTGCCCTCCGGTTTTCATGTCATACCTTTGGATCCCAAGGCAGATGGACCTACTGCAACTCGCACTTTAGATTTGGCCTCTACGCTTGAAGTAGGAACCAGTGGTGCTCGTGCCATTAATGACGCTGATGGGAATAGTTACAACCGTAGGTCAGTGCTAACCATAGCCTTCCAATTTACCTTTGAGAACCACTTGCGAGACAATGTGGCTGCAATGGCTCGCCAGTATGTGCGTAGTGTTGTAAGCTCTGTTCAGAGGGTAGCTATGGCTATTGCCCCATCCCCGCTCAGCTCCCAAATAGGACCAAAACCCCTTCCTGGATGTCCCGAGGCTCATACTTTGGCACGATGGATTTTCAGAAGCTTCAGGATTCACACTGGGGGAGAGCTCTTTCGAGTCGATATCTCATCTAGTGATGCTATTTTGAAGCAACTTTGGCGCCATTCGGATGCCATCATGTGCTGCTCTGTTAAAACAAATACGTCGCCAGTGTTCACCTTTGCAAACCAGGCTGGCCTTGACATGCTCGAAACCACTCTTGTTGCCCTCCAAGACATCATGCTCGACAAGATTCTAGACGAAGCTGGACGAAAATTTCTCTGCTCGGAGTTCTCCAAAATCATGCAACAGGGCTTTGCATATCTGCCAGCCGGAATATGTGCCTCCAGCATGGGGAGACCGGTGTCTTACGAGCAAGCTGTTGCATGGAAAGTTGTTAACGACGACGATTCCAATCACTGCCTTGCATTGATGTTCATGAACTGGTCCTTTATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009799 GO:0009855 GO:0009880 GO:0009888 GO:0009889 GO:0009908 GO:0009933 GO:0009943 GO:0009944 GO:0009955 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0080060 GO:0080090 GO:0090421 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox MEKHLA START

Protein Analysis

852

Amino Acids

93.08

Weight (kDa)

6.03

Isoelectric Point (pI)

50.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	24 - 82	1.4e-15	Homeodomain
START	PF01852	173 - 380	1e-50	START domain
MEKHLA	PF08670	706 - 850	1.8e-44	MEKHLA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014923)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30490 AT2G34710
fragaria_vesca	FvH4_3g10070 FvH4_3g10070
malus_domestica	MD05G1273700.v1.1 MD10G1253500.v1.1
prunus_persica	Prupe.4G090100_v2.0.a1
pyrus_communis	pycom05g25510 pycom10g20910
rosa_chinensis	RchiOBHm_Chr5g0015931
rosa_laevigata	RLG00000032232
rosa_multiflora	Rmu_sc0001878.1_g000010
rosa_roxburghii	Rroxscaffold_1G00060660
rosa_rugosa	Rorug05G0030500
rosa_samantha	Rh5BG122800
rosa_wichuraiana	Rw5G010700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	85
Acc36I	ACCTGC	2 cut(s)	85, 1201
AccB1I	GGYRCC	2 cut(s)	1718, 2217
AccB7I	CCANNNNNTGG	1 cut(s)	1373
AccBSI	CCGCTC	1 cut(s)	2065
AccI	GTMKAC	3 cut(s)	117, 570, 798
AccII	CGCG	1 cut(s)	732
AccIII	TCCGGA	1 cut(s)	604
AclWI	GGATC	3 cut(s)	1038, 1805, 1818
AcsI	RAATTY	2 cut(s)	1167, 2372
AcuI	CTGAAG	3 cut(s)	1083, 1668, 2128
AcvI	CACGTG	1 cut(s)	839
AcyI	GRCGYC	1 cut(s)	2218
AfaI	GTAC	3 cut(s)	74, 83, 1676
AfiI	CCNNNNNNNGG	8 cut(s)	150, 484, 550, 1001, 1373, 1376, 2096, 2451
AgeI	ACCGGT	1 cut(s)	2458
AgsI	TTSAA	3 cut(s)	1359, 1868, 2206
AhdI	GACNNNNNGTC	1 cut(s)	1212
AjnI	CCWGG	4 cut(s)	598, 931, 2095, 2286
AjuI	GAANNNNNNNTTGG	2 cut(s)	1182, 1214
Alw21I	GWGCWC	5 cut(s)	795, 1458, 1562, 1888, 2169
Alw26I	GTCTC	5 cut(s)	15, 521, 634, 1971, 2449
AlwI	GGATC	3 cut(s)	1038, 1805, 1818
AlwNI	CAGNNNCTG	2 cut(s)	382, 1222
Ama87I	CYCGRG	1 cut(s)	2105
Aor13HI	TCCGGA	1 cut(s)	604
AoxI	GGCC	4 cut(s)	925, 935, 1854, 2293
ApoI	RAATTY	2 cut(s)	1167, 2372
AseI	ATTAAT	1 cut(s)	1896
AsiGI	ACCGGT	1 cut(s)	2458
Asp700I	GAANNNNTTC	1 cut(s)	969
AspLEI	GCGC	3 cut(s)	7, 1727, 2220
AspS9I	GGNCC	8 cut(s)	577, 602, 926, 935, 1631, 1826, 2081, 2544
AsuC2I	CCSGG	2 cut(s)	30, 1460
AsuHPI	GGTGA	1 cut(s)	2266
AvaI	CYCGRG	1 cut(s)	2105
AvaII	GGWCC	6 cut(s)	577, 602, 1631, 1826, 2081, 2544
AxyI	CCTNAGG	1 cut(s)	1448
BaeGI	GKGCMC	2 cut(s)	131, 176
BamHI	GGATCC	1 cut(s)	1810
BanI	GGYRCC	2 cut(s)	1718, 2217
BanII	GRGCYC	3 cut(s)	687, 795, 2169
BauI	CACGAG	2 cut(s)	657, 1887
BbrPI	CACGTG	1 cut(s)	839
BbsI	GAAGAC	3 cut(s)	500, 882, 1115
Bbv12I	GWGCWC	5 cut(s)	795, 1458, 1562, 1888, 2169
BccI	CCATC	7 cut(s)	52, 1283, 1817, 1901, 2065, 2121, 2240
BceAI	ACGGC	1 cut(s)	895
BciT130I	CCWGG	4 cut(s)	600, 933, 2097, 2288
BcnI	CCSGG	2 cut(s)	30, 1460
BcoDI	GTCTC	5 cut(s)	15, 521, 634, 1971, 2449
BfaI	CTAG	7 cut(s)	680, 975, 1158, 1515, 1625, 2190, 2354
BfmI	CTRYAG	2 cut(s)	640, 1412
BfoI	RGCGCY	2 cut(s)	8, 2221
BfuAI	ACCTGC	2 cut(s)	85, 1201
BlpI	GCTNAGC	2 cut(s)	1728, 2066
Bme1390I	CCNGG	6 cut(s)	30, 600, 933, 1460, 2097, 2288
Bme18I	GGWCC	6 cut(s)	577, 602, 1631, 1826, 2081, 2544
BmeRI	GACNNNNNGTC	1 cut(s)	1212
BmeT110I	CYCGRG	1 cut(s)	2105
BmgT120I	GGNCC	8 cut(s)	577, 602, 926, 935, 1631, 1826, 2081, 2544
BmiI	GGNNCC	9 cut(s)	218, 578, 1012, 1342, 1632, 1720, 1812, 1876, 2219
BmrFI	CCNGG	6 cut(s)	30, 600, 933, 1460, 2097, 2288
BmrI	ACTGGG	3 cut(s)	583, 1339, 2166
BmuI	ACTGGG	3 cut(s)	583, 1339, 2166
BoxI	GACNNNNGTC	2 cut(s)	882, 2461
BpiI	GAAGAC	3 cut(s)	500, 882, 1115
BpmI	CTGGAG	2 cut(s)	251, 2428
Bpu1102I	GCTNAGC	2 cut(s)	1728, 2066
BpuEI	CTTGAG	2 cut(s)	767, 1134
BpuMI	CCSGG	2 cut(s)	30, 1460
BsaAI	YACGTR	1 cut(s)	839
BsaBI	GATNNNNATC	1 cut(s)	2511
BsaHI	GRCGYC	1 cut(s)	2218
BsaI	GGTCTC	2 cut(s)	521, 2449
BsaJI	CCNNGG	5 cut(s)	531, 932, 1395, 1815, 2106
BsaWI	WCCGGW	3 cut(s)	604, 1787, 2458
BsaXI	ACNNNNNCTCC	2 cut(s)	1368, 1398
Bsc4I	CCNNNNNNNGG	8 cut(s)	150, 484, 550, 1001, 1373, 1376, 2096, 2451
Bse118I	RCCGGY	1 cut(s)	2458
Bse1I	ACTGG	9 cut(s)	562, 578, 925, 1345, 1878, 2002, 2161, 2267, 2546
Bse21I	CCTNAGG	1 cut(s)	1448
Bse3DI	GCAATG	2 cut(s)	1308, 1998
Bse8I	GATNNNNATC	1 cut(s)	2511
BseAI	TCCGGA	1 cut(s)	604
BseBI	CCWGG	4 cut(s)	600, 933, 2097, 2288
BseDI	CCNNGG	5 cut(s)	531, 932, 1395, 1815, 2106
BseGI	GGATG	6 cut(s)	493, 597, 1267, 2057, 2105, 2233
BseJI	GATNNNNATC	1 cut(s)	2511
BseLI	CCNNNNNNNGG	8 cut(s)	150, 484, 550, 1001, 1373, 1376, 2096, 2451
BseMI	GCAATG	2 cut(s)	1308, 1998
BseMII	CTCAG	9 cut(s)	135, 137, 1084, 1118, 1193, 1439, 1455, 1742, 2080
BseNI	ACTGG	9 cut(s)	562, 578, 925, 1345, 1878, 2002, 2161, 2267, 2546
BseRI	GAGGAG	3 cut(s)	1392, 1616, 1764
BseSI	GKGCMC	2 cut(s)	131, 176
BseYI	CCCAGC	3 cut(s)	137, 471, 499
BsgI	GTGCAG	1 cut(s)	1711
Bsh1236I	CGCG	1 cut(s)	732
BshFI	GGCC	4 cut(s)	927, 937, 1856, 2295
BshNI	GGYRCC	2 cut(s)	1718, 2217
BshTI	ACCGGT	1 cut(s)	2458
BsiHKAI	GWGCWC	5 cut(s)	795, 1458, 1562, 1888, 2169
BsiHKCI	CYCGRG	1 cut(s)	2105
BsiSI	CCGG	7 cut(s)	29, 89, 605, 1459, 1788, 2430, 2459
BslFI	GGGAC	1 cut(s)	2088
BslI	CCNNNNNNNGG	8 cut(s)	150, 484, 550, 1001, 1373, 1376, 2096, 2451
BsmAI	GTCTC	5 cut(s)	15, 521, 634, 1971, 2449
BsmFI	GGGAC	1 cut(s)	2088
BsmI	GAATGC	1 cut(s)	1714
BsnI	GGCC	4 cut(s)	927, 937, 1856, 2295
Bso31I	GGTCTC	2 cut(s)	521, 2449
BsoBI	CYCGRG	1 cut(s)	2105
Bsp1286I	GDGCHC	8 cut(s)	131, 176, 687, 795, 1458, 1562, 1888, 2169
Bsp13I	TCCGGA	1 cut(s)	604
Bsp143I	GATC	4 cut(s)	204, 712, 1030, 1810
Bsp1720I	GCTNAGC	2 cut(s)	1728, 2066
Bsp68I	TCGCGA	1 cut(s)	732
BspANI	GGCC	4 cut(s)	927, 937, 1856, 2295
BspCNI	CTCAG	9 cut(s)	134, 138, 1083, 1117, 1194, 1440, 1456, 1741, 2079
BspEI	TCCGGA	1 cut(s)	604
BspFNI	CGCG	1 cut(s)	732
BspHI	TCATGA	2 cut(s)	432, 2535
BspLI	GGNNCC	9 cut(s)	218, 578, 1012, 1342, 1632, 1720, 1812, 1876, 2219
BspMAI	CTGCAG	2 cut(s)	644, 1416
BspMI	ACCTGC	2 cut(s)	85, 1201
BspPI	GGATC	3 cut(s)	1038, 1805, 1818
BspT107I	GGYRCC	2 cut(s)	1718, 2217
BspTNI	GGTCTC	2 cut(s)	521, 2449
BsrBI	CCGCTC	1 cut(s)	2065
BsrDI	GCAATG	2 cut(s)	1308, 1998
BsrFI	RCCGGY	1 cut(s)	2458
BsrI	ACTGG	9 cut(s)	562, 578, 925, 1345, 1878, 2002, 2161, 2267, 2546
BssAI	RCCGGY	1 cut(s)	2458
BssECI	CCNNGG	5 cut(s)	531, 932, 1395, 1815, 2106
BssMI	GATC	4 cut(s)	204, 712, 1030, 1810
BssNAI	GTATAC	1 cut(s)	799
BssNI	GRCGYC	1 cut(s)	2218
BssSI	CACGAG	2 cut(s)	657, 1887
BssT1I	CCWWGG	3 cut(s)	531, 1395, 1815
Bst1107I	GTATAC	1 cut(s)	799
Bst2BI	CACGAG	2 cut(s)	657, 1887
Bst2UI	CCWGG	4 cut(s)	600, 933, 2097, 2288
Bst4CI	ACNGT	5 cut(s)	274, 1268, 1591, 1686, 1925
Bst6I	CTCTTC	1 cut(s)	519
BstACI	GRCGYC	1 cut(s)	2218
BstAPI	GCANNNNNTGC	3 cut(s)	382, 1496, 1718
BstBAI	YACGTR	1 cut(s)	839
BstC8I	GCNNGC	7 cut(s)	664, 1581, 1723, 1999, 2293, 2427, 2475
BstENI	CCTNNNNNAGG	2 cut(s)	548, 1374
BstF5I	GGATG	6 cut(s)	493, 597, 1267, 2057, 2105, 2233
BstFNI	CGCG	1 cut(s)	732
BstH2I	RGCGCY	2 cut(s)	8, 2221
BstHHI	GCGC	3 cut(s)	7, 1727, 2220
BstKTI	GATC	4 cut(s)	207, 715, 1033, 1813
BstMAI	GTCTC	5 cut(s)	15, 521, 634, 1971, 2449
BstMBI	GATC	4 cut(s)	204, 712, 1030, 1810
BstNI	CCWGG	4 cut(s)	600, 933, 2097, 2288
BstNSI	RCATGY	3 cut(s)	805, 1506, 2305
BstPAI	GACNNNNGTC	2 cut(s)	882, 2461
BstSCI	CCNGG	6 cut(s)	28, 598, 931, 1458, 2095, 2286
BstSFI	CTRYAG	2 cut(s)	640, 1412
BstSLI	GKGCMC	2 cut(s)	131, 176
BstUI	CGCG	1 cut(s)	732
BstV2I	GAAGAC	3 cut(s)	500, 882, 1115
BstX2I	RGATCY	1 cut(s)	1810
BstXI	CCANNNNNNTGG	2 cut(s)	587, 826
BstYI	RGATCY	1 cut(s)	1810
BstZ17I	GTATAC	1 cut(s)	799
Bsu36I	CCTNAGG	1 cut(s)	1448
BsuRI	GGCC	4 cut(s)	927, 937, 1856, 2295
BtgZI	GCGATG	1 cut(s)	308
BtsCI	GGATG	6 cut(s)	493, 597, 1267, 2057, 2105, 2233
BtsI	GCAGTG	3 cut(s)	649, 1699, 2515
BtsIMutI	CAGTG	8 cut(s)	279, 649, 1699, 1885, 1938, 2154, 2274, 2515
BtuMI	TCGCGA	1 cut(s)	732
BveI	ACCTGC	2 cut(s)	85, 1201
Cac8I	GCNNGC	7 cut(s)	664, 1581, 1723, 1999, 2293, 2427, 2475
CaiI	CAGNNNCTG	2 cut(s)	382, 1222
CciI	TCATGA	2 cut(s)	432, 2535
CfoI	GCGC	3 cut(s)	7, 1727, 2220
Cfr10I	RCCGGY	1 cut(s)	2458
Cfr13I	GGNCC	8 cut(s)	577, 602, 926, 935, 1631, 1826, 2081, 2544
CseI	GACGC	2 cut(s)	1055, 1910
Csp6I	GTAC	3 cut(s)	73, 82, 1675
CspAI	ACCGGT	1 cut(s)	2458
CspCI	CAANNNNNGTGG	4 cut(s)	625, 660, 2302, 2337
CviQI	GTAC	3 cut(s)	73, 82, 1675
DinI	GGCGCC	1 cut(s)	2219
DpnI	GATC	4 cut(s)	206, 714, 1032, 1812
DpnII	GATC	4 cut(s)	204, 712, 1030, 1810
DriI	GACNNNNNGTC	1 cut(s)	1212
Eam1104I	CTCTTC	1 cut(s)	519
Eam1105I	GACNNNNNGTC	1 cut(s)	1212
EarI	CTCTTC	1 cut(s)	519
Ecl136II	GAGCTC	2 cut(s)	793, 2167
Eco130I	CCWWGG	3 cut(s)	531, 1395, 1815
Eco24I	GRGCYC	3 cut(s)	687, 795, 2169
Eco31I	GGTCTC	2 cut(s)	521, 2449
Eco32I	GATATC	1 cut(s)	2182
Eco47I	GGWCC	6 cut(s)	577, 602, 1631, 1826, 2081, 2544
Eco53kI	GAGCTC	2 cut(s)	793, 2167
Eco57I	CTGAAG	3 cut(s)	1083, 1668, 2128
Eco72I	CACGTG	1 cut(s)	839
Eco81I	CCTNAGG	1 cut(s)	1448
Eco88I	CYCGRG	1 cut(s)	2105
EcoICRI	GAGCTC	2 cut(s)	793, 2167
EcoNI	CCTNNNNNAGG	2 cut(s)	548, 1374
EcoO109I	RGGNCCY	1 cut(s)	1631
EcoRII	CCWGG	4 cut(s)	598, 931, 2095, 2286
EcoRV	GATATC	1 cut(s)	2182
EcoT14I	CCWWGG	3 cut(s)	531, 1395, 1815
EcoT22I	ATGCAT	1 cut(s)	1492
EcoT38I	GRGCYC	3 cut(s)	687, 795, 2169
EgeI	GGCGCC	1 cut(s)	2219
EheI	GGCGCC	1 cut(s)	2219
ErhI	CCWWGG	3 cut(s)	531, 1395, 1815
FaqI	GGGAC	1 cut(s)	2088
FauI	CCCGC	4 cut(s)	38, 641, 763, 2070
FauNDI	CATATG	1 cut(s)	1522
FblI	GTMKAC	3 cut(s)	117, 570, 798
FokI	GGATG	6 cut(s)	500, 604, 1274, 2044, 2112, 2240
FriOI	GRGCYC	3 cut(s)	687, 795, 2169
FspBI	CTAG	7 cut(s)	680, 975, 1158, 1515, 1625, 2190, 2354
GlaI	GCGC	3 cut(s)	6, 1726, 2219
GsaI	CCCAGC	3 cut(s)	141, 475, 503
GsuI	CTGGAG	2 cut(s)	251, 2428
HaeII	RGCGCY	2 cut(s)	8, 2221
HaeIII	GGCC	4 cut(s)	927, 937, 1856, 2295
HapII	CCGG	7 cut(s)	29, 89, 605, 1459, 1788, 2430, 2459
HgaI	GACGC	2 cut(s)	1055, 1910
HhaI	GCGC	3 cut(s)	7, 1727, 2220
Hin1I	GRCGYC	1 cut(s)	2218
Hin6I	GCGC	3 cut(s)	5, 1725, 2218
HinP1I	GCGC	3 cut(s)	5, 1725, 2218
HincII	GTYRAC	2 cut(s)	571, 2497
HindII	GTYRAC	2 cut(s)	571, 2497
HindIII	AAGCTT	1 cut(s)	2140
HpaI	GTTAAC	1 cut(s)	2497
HpaII	CCGG	7 cut(s)	29, 89, 605, 1459, 1788, 2430, 2459
HphI	GGTGA	1 cut(s)	2266
Hpy166II	GTNNAC	7 cut(s)	84, 118, 277, 571, 799, 2274, 2497
Hpy8I	GTNNAC	7 cut(s)	84, 118, 277, 571, 799, 2274, 2497
Hpy99I	CGWCG	3 cut(s)	2266, 2504, 2507
HpyAV	CCTTC	4 cut(s)	144, 244, 1957, 2102
HpyCH4III	ACNGT	5 cut(s)	274, 1268, 1591, 1686, 1925
HpyCH4IV	ACGT	3 cut(s)	75, 838, 2261
HpySE526I	ACGT	3 cut(s)	75, 838, 2261
Hsp92I	GRCGYC	1 cut(s)	2218
HspAI	GCGC	3 cut(s)	5, 1725, 2218
KasI	GGCGCC	1 cut(s)	2217
Kpn2I	TCCGGA	1 cut(s)	604
KspAI	GTTAAC	1 cut(s)	2497
Kzo9I	GATC	4 cut(s)	204, 712, 1030, 1810
LmnI	GCTCC	7 cut(s)	91, 146, 1053, 1453, 1594, 1777, 2075
MaeI	CTAG	7 cut(s)	680, 975, 1158, 1515, 1625, 2190, 2354
MaeII	ACGT	3 cut(s)	75, 838, 2261
MaeIII	GTNAC	4 cut(s)	839, 1074, 1303, 1916
MalI	GATC	4 cut(s)	206, 714, 1032, 1812
MbiI	CCGCTC	1 cut(s)	2065
MboI	GATC	4 cut(s)	204, 712, 1030, 1810
MboII	GAAGA	8 cut(s)	249, 326, 500, 536, 887, 1120, 1310, 1661
MfeI	CAATTG	1 cut(s)	786
MflI	RGATCY	1 cut(s)	1810
MhlI	GDGCHC	8 cut(s)	131, 176, 687, 795, 1458, 1562, 1888, 2169
MluCI	AATT	5 cut(s)	786, 1167, 1359, 1952, 2372
Mly113I	GGCGCC	1 cut(s)	2218
MlyI	GAGTC	6 cut(s)	17, 123, 258, 431, 1163, 2184
MmeI	TCCRAC	4 cut(s)	87, 210, 1015, 1250
Mph1103I	ATGCAT	1 cut(s)	1492
MroI	TCCGGA	1 cut(s)	604
MroXI	GAANNNNTTC	1 cut(s)	969
MseI	TTAA	9 cut(s)	308, 755, 1236, 1248, 1317, 1509, 1896, 2250, 2496
MslI	CAYNNNNRTG	1 cut(s)	2226
MspA1I	CMGCKG	3 cut(s)	42, 247, 503
MspI	CCGG	7 cut(s)	29, 89, 605, 1459, 1788, 2430, 2459
MspR9I	CCNGG	6 cut(s)	30, 600, 933, 1460, 2097, 2288
MunI	CAATTG	1 cut(s)	786
Mva1269I	GAATGC	1 cut(s)	1714
MvaI	CCWGG	4 cut(s)	600, 933, 2097, 2288
MvnI	CGCG	1 cut(s)	732
NarI	GGCGCC	1 cut(s)	2218
NciI	CCSGG	2 cut(s)	30, 1460
NdeI	CATATG	1 cut(s)	1522
NdeII	GATC	4 cut(s)	204, 712, 1030, 1810
NlaIV	GGNNCC	9 cut(s)	218, 578, 1012, 1342, 1632, 1720, 1812, 1876, 2219
NmeAIII	GCCGAG	1 cut(s)	260
NmuCI	GTSAC	2 cut(s)	839, 1074
NruI	TCGCGA	1 cut(s)	732
NsiI	ATGCAT	1 cut(s)	1492
NspI	RCATGY	3 cut(s)	805, 1506, 2305
PagI	TCATGA	2 cut(s)	432, 2535
PaqCI	CACCTGC	1 cut(s)	85
PcsI	WCGNNNNNNNCGW	2 cut(s)	736, 1053
PctI	GAATGC	1 cut(s)	1714
PdmI	GAANNNNTTC	1 cut(s)	969
PfeI	GAWTC	8 cut(s)	62, 254, 608, 1085, 1754, 2149, 2350, 2507
PflFI	GACNNNGTC	1 cut(s)	575
PflMI	CCANNNNNTGG	1 cut(s)	1373
PfoI	TCCNGGA	1 cut(s)	2095
PinAI	ACCGGT	1 cut(s)	2458
PleI	GAGTC	6 cut(s)	17, 122, 258, 430, 1162, 2183
PluTI	GGCGCC	1 cut(s)	2221
PmaCI	CACGTG	1 cut(s)	839
PmlI	CACGTG	1 cut(s)	839
PpsI	GAGTC	6 cut(s)	17, 122, 258, 430, 1162, 2183
Ppu21I	YACGTR	1 cut(s)	839
PpuMI	RGGWCCY	1 cut(s)	1631
PshAI	GACNNNNGTC	2 cut(s)	882, 2461
PshBI	ATTAAT	1 cut(s)	1896
Psp124BI	GAGCTC	2 cut(s)	795, 2169
Psp5II	RGGWCCY	1 cut(s)	1631
Psp6I	CCWGG	4 cut(s)	598, 931, 2095, 2286
PspCI	CACGTG	1 cut(s)	839
PspFI	CCCAGC	3 cut(s)	137, 471, 499
PspGI	CCWGG	4 cut(s)	598, 931, 2095, 2286
PspN4I	GGNNCC	9 cut(s)	218, 578, 1012, 1342, 1632, 1720, 1812, 1876, 2219
PspPI	GGNCC	8 cut(s)	577, 602, 926, 935, 1631, 1826, 2081, 2544
PspPPI	RGGWCCY	1 cut(s)	1631
PsrI	GAACNNNNNNTAC	2 cut(s)	2013, 2045
PstI	CTGCAG	2 cut(s)	644, 1416
PstNI	CAGNNNCTG	2 cut(s)	382, 1222
PsuI	RGATCY	1 cut(s)	1810
PsyI	GACNNNGTC	1 cut(s)	575
PvuII	CAGCTG	1 cut(s)	503
RruI	TCGCGA	1 cut(s)	732
RsaI	GTAC	3 cut(s)	74, 83, 1676
RsaNI	GTAC	3 cut(s)	73, 82, 1675
RseI	CAYNNNNRTG	1 cut(s)	2226
SacI	GAGCTC	2 cut(s)	795, 2169
SalI	GTCGAC	1 cut(s)	569
SaqAI	TTAA	9 cut(s)	308, 755, 1236, 1248, 1317, 1509, 1896, 2250, 2496
Sau3AI	GATC	4 cut(s)	204, 712, 1030, 1810
Sau96I	GGNCC	8 cut(s)	577, 602, 926, 935, 1631, 1826, 2081, 2544
SchI	GAGTC	6 cut(s)	17, 123, 258, 431, 1163, 2184
ScrFI	CCNGG	6 cut(s)	30, 600, 933, 1460, 2097, 2288
SduI	GDGCHC	8 cut(s)	131, 176, 687, 795, 1458, 1562, 1888, 2169
SfcI	CTRYAG	2 cut(s)	640, 1412
SfoI	GGCGCC	1 cut(s)	2219
SinI	GGWCC	6 cut(s)	577, 602, 1631, 1826, 2081, 2544
SmiMI	CAYNNNNRTG	1 cut(s)	2226
SmlI	CTYRAG	2 cut(s)	746, 1149
SmoI	CTYRAG	2 cut(s)	746, 1149
Sse9I	AATT	5 cut(s)	786, 1167, 1359, 1952, 2372
SspDI	GGCGCC	1 cut(s)	2217
SspI	AATATT	1 cut(s)	1570
SspMI	CTAG	7 cut(s)	680, 975, 1158, 1515, 1625, 2190, 2354
SstI	GAGCTC	2 cut(s)	795, 2169
StyD4I	CCNGG	6 cut(s)	28, 598, 931, 1458, 2095, 2286
StyI	CCWWGG	3 cut(s)	531, 1395, 1815
TaaI	ACNGT	5 cut(s)	274, 1268, 1591, 1686, 1925
TaiI	ACGT	3 cut(s)	78, 841, 2264
TaqI	TCGA	7 cut(s)	65, 262, 570, 2173, 2178, 2307, 2343
TasI	AATT	5 cut(s)	786, 1167, 1359, 1952, 2372
TatI	WGTACW	1 cut(s)	1674
TauI	GCSGC	2 cut(s)	45, 836
TfiI	GAWTC	8 cut(s)	62, 254, 608, 1085, 1754, 2149, 2350, 2507
Tru1I	TTAA	9 cut(s)	308, 755, 1236, 1248, 1317, 1509, 1896, 2250, 2496
Tru9I	TTAA	9 cut(s)	308, 755, 1236, 1248, 1317, 1509, 1896, 2250, 2496
TscAI	CASTG	8 cut(s)	279, 649, 1699, 1885, 1938, 2161, 2274, 2522
TseFI	GTSAC	2 cut(s)	839, 1074
Tsp45I	GTSAC	2 cut(s)	839, 1074
TspDTI	ATGAA	8 cut(s)	311, 386, 608, 1098, 1767, 1784, 2524, 2552
TspRI	CASTG	8 cut(s)	279, 649, 1699, 1885, 1938, 2161, 2274, 2522
Tth111I	GACNNNGTC	1 cut(s)	575
Van91I	CCANNNNNTGG	1 cut(s)	1373
VpaK11BI	GGWCC	6 cut(s)	577, 602, 1631, 1826, 2081, 2544
VspI	ATTAAT	1 cut(s)	1896
XagI	CCTNNNNNAGG	2 cut(s)	548, 1374
XapI	RAATTY	2 cut(s)	1167, 2372
XbaI	TCTAGA	1 cut(s)	2353
XceI	RCATGY	3 cut(s)	805, 1506, 2305
XmiI	GTMKAC	3 cut(s)	117, 570, 798
XmnI	GAANNNNTTC	1 cut(s)	969
XspI	CTAG	7 cut(s)	680, 975, 1158, 1515, 1625, 2190, 2354
Zsp2I	ATGCAT	1 cut(s)	1492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.