Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0014923

Physical Location & Seq

Genomic Coordinates Forward (+)

26879872 .. 26885367

Gene Structure 5496 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g25510.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGCGCTGCTAATGCATCGAGACTCGCCTGGAAGCAGCAGCGGCGGGAGGCAGCAGATGGATTCGAGCAAGTACGTTCGGTACACGCCGGAGCAGGTGGAGGCGTTGGAGAGAGTCTACTCAGAGTGCCCAAAGCCCAGCTCTCTGAAAAGGCAGCAGCTCATCAGAGAGTGCCCCATTCTCTCCAACATTGAGCCTAAACAGATCAAAGTCTGGTTCCAGAACCGCAGATGCCGAGAGAAGCAGCGGAAGGAATCTTCTCGGCTCCAGACAGTGAACAGAAAGCTATCTGCAATGAACAAGCTGTTAATGGAAGAAAATGACCGCTTACAGAAGCAAGTTTCTCATTTGGTTTATGAGAACGGATTTATGAAGCAGCAACTGCATACCGCATCGGGAACGACCACAGACAATAGCTGCGAGTCTGTGGTCATGAGTGGTCAGCACCAACAACAGCAAAACCCAACTCCCCAGCACCCCAAAAGGGATGCTAACAACCCAGCTGGTCTTCTTGCAATAGCAGAAGAGACCCTGGCAGAGTTCCTTTCTAAGGCTACCGGAACTGCTGTCGACTGGGTCCAGATGATTGGGATGAAGCCTGGTCCGGATTCTATTGGCATCGTCGCTGTCTCCCGCAACTGCAGTGGAGTAGCAGCACGAGCTTGCGGTCTTGTGAGCCTAGAGCCCACAAAGGTCGCCGAAATCCTCAAAGATCGTAAGTCTTGGTTTCGCGATTGCCGATGCCTTGAGGTGTTAAGTTTAATCCCTGCGGGGAATGGTGGAACAATTGAGCTCGTATACATGCAGACTTATGCACCCACAACATTGGCTGCGGCACGTGACTTTTGGACGCTAAGATATACTACAAGTTTGGAAGACGGCAGTCTTGTGGTATGCGAGAGGTCGTTGACTTCTTCTACTGGTGGCCCAACAGGGCCAACATCGGCGAGTTTTGTCAGAGCTGAGATGCTTCCGAGTGGCTATCTTATCCGACCTTGTGAGGGTGGTGGCTCCATTATCAACATTGTTGATCATGTTGATTTAGATGCCTGGAGCGTTCCCGAAGTTCTCAGGTCACTTTATGAATCCTGCAAAATCCTTGCTCAAAAGACGACCATTTCTGCCTTGCGACACATACGACAGATAGCTCAAGAGTCTAGTGGAGAAATTCAGCATGGTGGAGGTCGCCAACCTGCTGTGCTGAGGACATTTTGTCAGAGGCTGTGCAGTGGGTTTAACGATGCTGTTAATGGGTTTGCTGATGACGGTTGGACACTAATGGGTAGTGATGGTGTGGAAGATGTTACCATTGCGATTAACTCATCTCCAAACAAAGTGCTTGGTTCGCAGTATAACACATCTATTTTCTCACCCTTTGGAGGAGGGGTGTTGTGTGCCAAGGCATCAATGCTGCTGCAGAGTGTTCCTCCTGCTTTGCTTGTTCGTTTTCTGAGGGAGCACCGCTCAGAGTGGGCTGACTATGGGGTTGATGCATACTCTGCGGCATGTCTCAAAGCTAGTCCATATGCAGTTCCTCTTGCAAGAGGTGGTGGCTTTCCTACCACCCAGAATATTTTACCGCTTGCACAAACTGTGGAGCATGAGGAGTTTTTAGAGGTAGTTCGGCTAGAGGGTCCTGCATTCTCTCCTGAAGATGTAGCTTTGGCACAGGATATGTACTTATTACAGTTGTGCAGTGGAGTTGATGAGAATGCAGTTGGTGCCTGTGCTCAGCTTGTCTTCGCACCTATCGATGAATCATTTGCTGATGATGCTCCTTTGTTGCCCTCTGGTTTTCATGTCATACCTTTGGATCCCAAGGCAGATGGACCTACTGCATCTCGCACATTAGATTTGGCCTCTACCCTTGAAATAGGAACCAGTGGTGCTCGCGCCGTTAATGACCCTGATGGGAATGGTTACAACCTTAGGTCAGTGCTAACCATAGCCTTCCAATTTACCTTTGAGAACCACTTGCGAGACAATGTGGCTGCTATGGCTCGCCAGTACGTGCGTAGTGTTGTAAGCTCTGTTCAGAGGGTAGCTATGGCTATTGCCCCTTCCAGGCTCAGCTCGCAAATAGGACCAAAGCCCCTTCCTGGATCTCCCGAAGCTCATACTTTGGCACGATGGATTTTCAGAAGCTACAGGATCCACACTGGGGGAGAGCTATTTCGTGTCGATATCTCATCTGGTGATGCTGTTTTGAAACAACTTTGGCGCCATTCGGATGCCATCATGTGCTGCTCTGTGAAAACAAATGCATCTCCAGTGTTCACCTTTGCAAACCAGGCTGGCCTTGACATGCTCGAAACTACCCTTGTTGCCCTCCAAGATATCACGCTTGACAAGATTCTAGACGAAGCTGGACGGAAACTTCTCTGCTCGGAGTTCTCCAAAATCACGCAGCAGGGCTTTGCATATCTGCCAGCCGGAATATGTGCATCCAGCATGGGTAGGCCGGTGTCCTACGAGCAAGCTGTTGCATGGAAAGTTGTTAACGATGACGATTCCAATCACTGCCTGGCATTGATGTTCATGAACTGGTCCTTCGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009799 GO:0009855 GO:0009880 GO:0009888 GO:0009889 GO:0009908 GO:0009933 GO:0009943 GO:0009944 GO:0009955 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0080060 GO:0080090 GO:0090421 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox MEKHLA START

Protein Analysis

853

Amino Acids

92.95

Weight (kDa)

6.03

Isoelectric Point (pI)

50.15

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014923)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30490 AT2G34710
fragaria_vesca	FvH4_3g10070 FvH4_3g10070
malus_domestica	MD05G1273700.v1.1 MD10G1253500.v1.1
prunus_persica	Prupe.4G090100_v2.0.a1
pyrus_communis	pycom05g25510 pycom10g20910
rosa_chinensis	RchiOBHm_Chr5g0015931
rosa_laevigata	RLG00000032232
rosa_multiflora	Rmu_sc0001878.1_g000010
rosa_roxburghii	Rroxscaffold_1G00060660
rosa_rugosa	Rorug05G0030500
rosa_samantha	Rh5BG122800
rosa_wichuraiana	Rw5G010700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	85
Acc36I	ACCTGC	2 cut(s)	85, 1201
AccB1I	GGYRCC	2 cut(s)	1721, 2220
AccBSI	CCGCTC	1 cut(s)	1464
AccI	GTMKAC	3 cut(s)	117, 570, 798
AccII	CGCG	2 cut(s)	732, 1893
AccIII	TCCGGA	1 cut(s)	604
AclWI	GGATC	5 cut(s)	1808, 1821, 2110, 2146, 2159
AcsI	RAATTY	1 cut(s)	1167
AcuI	CTGAAG	1 cut(s)	1671
AcvI	CACGTG	1 cut(s)	839
AcyI	GRCGYC	1 cut(s)	2221
AfaI	GTAC	4 cut(s)	74, 83, 1679, 2009
AfiI	CCNNNNNNNGG	7 cut(s)	484, 550, 1001, 1379, 2064, 2099, 2161
AgsI	TTSAA	2 cut(s)	1871, 2209
AhdI	GACNNNNNGTC	1 cut(s)	1212
AjnI	CCWGG	8 cut(s)	28, 531, 598, 1049, 2063, 2098, 2289, 2523
Alw21I	GWGCWC	4 cut(s)	795, 1461, 1732, 1891
Alw26I	GTCTC	5 cut(s)	15, 521, 634, 1514, 1974
AlwI	GGATC	5 cut(s)	1808, 1821, 2110, 2146, 2159
AlwNI	CAGNNNCTG	2 cut(s)	382, 1222
Aor13HI	TCCGGA	1 cut(s)	604
AoxI	GGCC	5 cut(s)	925, 935, 1857, 2296, 2459
ApoI	RAATTY	1 cut(s)	1167
AspLEI	GCGC	3 cut(s)	7, 1895, 2223
AspS9I	GGNCC	8 cut(s)	577, 602, 926, 935, 1634, 1829, 2084, 2547
AsuHPI	GGTGA	3 cut(s)	1362, 2207, 2269
AvaII	GGWCC	6 cut(s)	577, 602, 1634, 1829, 2084, 2547
AxyI	CCTNAGG	1 cut(s)	1928
BaeGI	GKGCMC	2 cut(s)	131, 176
BamHI	GGATCC	2 cut(s)	1813, 2151
BanI	GGYRCC	2 cut(s)	1721, 2220
BanII	GRGCYC	2 cut(s)	687, 795
BauI	CACGAG	1 cut(s)	657
BbrPI	CACGTG	1 cut(s)	839
BbsI	GAAGAC	3 cut(s)	500, 882, 1732
Bbv12I	GWGCWC	4 cut(s)	795, 1461, 1732, 1891
BbvCI	CCTCAGC	1 cut(s)	1202
BccI	CCATC	6 cut(s)	52, 1283, 1820, 1904, 2124, 2243
BceAI	ACGGC	2 cut(s)	895, 1880
BciT130I	CCWGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
BclI	TGATCA	1 cut(s)	1030
BcoDI	GTCTC	5 cut(s)	15, 521, 634, 1514, 1974
BfaI	CTAG	5 cut(s)	680, 1158, 1518, 1628, 2357
BfmI	CTRYAG	3 cut(s)	640, 1415, 2146
BfoI	RGCGCY	2 cut(s)	8, 2224
BfuAI	ACCTGC	2 cut(s)	85, 1201
BlpI	GCTNAGC	2 cut(s)	1731, 2069
Bme1390I	CCNGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
Bme18I	GGWCC	6 cut(s)	577, 602, 1634, 1829, 2084, 2547
BmeRI	GACNNNNNGTC	1 cut(s)	1212
BmgT120I	GGNCC	8 cut(s)	577, 602, 926, 935, 1634, 1829, 2084, 2547
BmrFI	CCNGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
BmrI	ACTGGG	2 cut(s)	583, 2169
BmuI	ACTGGG	2 cut(s)	583, 2169
BoxI	GACNNNNGTC	1 cut(s)	882
BpiI	GAAGAC	3 cut(s)	500, 882, 1732
BplI	GAGNNNNNCTC	4 cut(s)	1411, 1443, 1448, 1480
BpmI	CTGGAG	3 cut(s)	251, 1072, 2253
Bpu10I	CCTNAGC	1 cut(s)	1202
Bpu1102I	GCTNAGC	2 cut(s)	1731, 2069
BpuEI	CTTGAG	2 cut(s)	767, 1134
Bsa29I	ATCGAT	1 cut(s)	1752
BsaAI	YACGTR	2 cut(s)	839, 2011
BsaBI	GATNNNNATC	1 cut(s)	2514
BsaHI	GRCGYC	1 cut(s)	2221
BsaI	GGTCTC	1 cut(s)	521
BsaJI	CCNNGG	3 cut(s)	531, 1398, 1818
BsaWI	WCCGGW	2 cut(s)	557, 604
BsaXI	ACNNNNNCTCC	2 cut(s)	1371, 1401
Bsc4I	CCNNNNNNNGG	7 cut(s)	484, 550, 1001, 1379, 2064, 2099, 2161
Bse118I	RCCGGY	1 cut(s)	2461
Bse1I	ACTGG	7 cut(s)	578, 925, 1881, 2005, 2164, 2270, 2549
Bse21I	CCTNAGG	1 cut(s)	1928
Bse3DI	GCAATG	2 cut(s)	300, 1308
Bse8I	GATNNNNATC	1 cut(s)	2514
BseAI	TCCGGA	1 cut(s)	604
BseBI	CCWGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
BseCI	ATCGAT	1 cut(s)	1752
BseDI	CCNNGG	3 cut(s)	531, 1398, 1818
BseGI	GGATG	4 cut(s)	493, 597, 2236, 2444
BseJI	GATNNNNATC	1 cut(s)	2514
BseLI	CCNNNNNNNGG	7 cut(s)	484, 550, 1001, 1379, 2064, 2099, 2161
BseMI	GCAATG	2 cut(s)	300, 1308
BseMII	CTCAG	8 cut(s)	135, 954, 1084, 1193, 1442, 1479, 1745, 2083
BseNI	ACTGG	7 cut(s)	578, 925, 1881, 2005, 2164, 2270, 2549
BseRI	GAGGAG	2 cut(s)	1395, 1619
BseSI	GKGCMC	2 cut(s)	131, 176
BseYI	CCCAGC	3 cut(s)	137, 471, 499
BsgI	GTGCAG	2 cut(s)	1246, 1714
Bsh1236I	CGCG	2 cut(s)	732, 1893
BshFI	GGCC	5 cut(s)	927, 937, 1859, 2298, 2461
BshNI	GGYRCC	2 cut(s)	1721, 2220
BshVI	ATCGAT	1 cut(s)	1752
BsiHKAI	GWGCWC	4 cut(s)	795, 1461, 1732, 1891
BsiSI	CCGG	5 cut(s)	89, 558, 605, 2433, 2462
BslI	CCNNNNNNNGG	7 cut(s)	484, 550, 1001, 1379, 2064, 2099, 2161
BsmAI	GTCTC	5 cut(s)	15, 521, 634, 1514, 1974
BsmI	GAATGC	2 cut(s)	1640, 1717
BsnI	GGCC	5 cut(s)	927, 937, 1859, 2298, 2461
Bso31I	GGTCTC	1 cut(s)	521
Bsp1286I	GDGCHC	7 cut(s)	131, 176, 687, 795, 1461, 1732, 1891
Bsp13I	TCCGGA	1 cut(s)	604
Bsp143I	GATC	6 cut(s)	204, 712, 1030, 1813, 2102, 2151
Bsp1720I	GCTNAGC	2 cut(s)	1731, 2069
Bsp68I	TCGCGA	1 cut(s)	732
BspANI	GGCC	5 cut(s)	927, 937, 1859, 2298, 2461
BspCNI	CTCAG	8 cut(s)	134, 955, 1083, 1194, 1443, 1478, 1744, 2082
BspDI	ATCGAT	1 cut(s)	1752
BspEI	TCCGGA	1 cut(s)	604
BspFNI	CGCG	2 cut(s)	732, 1893
BspHI	TCATGA	2 cut(s)	432, 2538
BspMAI	CTGCAG	2 cut(s)	644, 1419
BspMI	ACCTGC	2 cut(s)	85, 1201
BspPI	GGATC	5 cut(s)	1808, 1821, 2110, 2146, 2159
BspT107I	GGYRCC	2 cut(s)	1721, 2220
BspTNI	GGTCTC	1 cut(s)	521
BsrBI	CCGCTC	1 cut(s)	1464
BsrDI	GCAATG	2 cut(s)	300, 1308
BsrFI	RCCGGY	1 cut(s)	2461
BsrI	ACTGG	7 cut(s)	578, 925, 1881, 2005, 2164, 2270, 2549
BssAI	RCCGGY	1 cut(s)	2461
BssECI	CCNNGG	3 cut(s)	531, 1398, 1818
BssMI	GATC	6 cut(s)	204, 712, 1030, 1813, 2102, 2151
BssNAI	GTATAC	1 cut(s)	799
BssNI	GRCGYC	1 cut(s)	2221
BssSI	CACGAG	1 cut(s)	657
BssT1I	CCWWGG	2 cut(s)	1398, 1818
Bst1107I	GTATAC	1 cut(s)	799
Bst2BI	CACGAG	1 cut(s)	657
Bst2UI	CCWGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
Bst4CI	ACNGT	4 cut(s)	274, 1268, 1594, 1689
Bst6I	CTCTTC	1 cut(s)	519
BstACI	GRCGYC	1 cut(s)	2221
BstAPI	GCANNNNNTGC	3 cut(s)	382, 1499, 1721
BstBAI	YACGTR	2 cut(s)	839, 2011
BstC8I	GCNNGC	8 cut(s)	664, 1584, 1891, 2002, 2075, 2296, 2430, 2478
BstENI	CCTNNNNNAGG	1 cut(s)	548
BstF5I	GGATG	4 cut(s)	493, 597, 2236, 2444
BstFNI	CGCG	2 cut(s)	732, 1893
BstH2I	RGCGCY	2 cut(s)	8, 2224
BstHHI	GCGC	3 cut(s)	7, 1895, 2223
BstKTI	GATC	6 cut(s)	207, 715, 1033, 1816, 2105, 2154
BstMAI	GTCTC	5 cut(s)	15, 521, 634, 1514, 1974
BstMBI	GATC	6 cut(s)	204, 712, 1030, 1813, 2102, 2151
BstNI	CCWGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
BstNSI	RCATGY	3 cut(s)	805, 1509, 2308
BstPAI	GACNNNNGTC	1 cut(s)	882
BstSCI	CCNGG	8 cut(s)	28, 531, 598, 1049, 2063, 2098, 2289, 2523
BstSFI	CTRYAG	3 cut(s)	640, 1415, 2146
BstSLI	GKGCMC	2 cut(s)	131, 176
BstUI	CGCG	2 cut(s)	732, 1893
BstV2I	GAAGAC	3 cut(s)	500, 882, 1732
BstX2I	RGATCY	3 cut(s)	1813, 2102, 2151
BstXI	CCANNNNNNTGG	2 cut(s)	587, 826
BstYI	RGATCY	3 cut(s)	1813, 2102, 2151
BstZ17I	GTATAC	1 cut(s)	799
Bsu15I	ATCGAT	1 cut(s)	1752
Bsu36I	CCTNAGG	1 cut(s)	1928
BsuRI	GGCC	5 cut(s)	927, 937, 1859, 2298, 2461
BsuTUI	ATCGAT	1 cut(s)	1752
BtsCI	GGATG	4 cut(s)	493, 597, 2236, 2444
BtsI	GCAGTG	4 cut(s)	649, 1234, 1702, 2518
BtsIMutI	CAGTG	9 cut(s)	279, 649, 1234, 1702, 1888, 1941, 2157, 2277, 2518
BtuMI	TCGCGA	1 cut(s)	732
BveI	ACCTGC	2 cut(s)	85, 1201
Cac8I	GCNNGC	8 cut(s)	664, 1584, 1891, 2002, 2075, 2296, 2430, 2478
CaiI	CAGNNNCTG	2 cut(s)	382, 1222
CciI	TCATGA	2 cut(s)	432, 2538
CfoI	GCGC	3 cut(s)	7, 1895, 2223
Cfr10I	RCCGGY	1 cut(s)	2461
Cfr13I	GGNCC	8 cut(s)	577, 602, 926, 935, 1634, 1829, 2084, 2547
ClaI	ATCGAT	1 cut(s)	1752
CseI	GACGC	1 cut(s)	859
Csp6I	GTAC	4 cut(s)	73, 82, 1678, 2008
CspCI	CAANNNNNGTGG	2 cut(s)	625, 660
CviQI	GTAC	4 cut(s)	73, 82, 1678, 2008
DinI	GGCGCC	1 cut(s)	2222
DpnI	GATC	6 cut(s)	206, 714, 1032, 1815, 2104, 2153
DpnII	GATC	6 cut(s)	204, 712, 1030, 1813, 2102, 2151
DriI	GACNNNNNGTC	1 cut(s)	1212
Eam1104I	CTCTTC	1 cut(s)	519
Eam1105I	GACNNNNNGTC	1 cut(s)	1212
EarI	CTCTTC	1 cut(s)	519
Ecl136II	GAGCTC	1 cut(s)	793
Eco130I	CCWWGG	2 cut(s)	1398, 1818
Eco24I	GRGCYC	2 cut(s)	687, 795
Eco31I	GGTCTC	1 cut(s)	521
Eco32I	GATATC	2 cut(s)	2185, 2338
Eco47I	GGWCC	6 cut(s)	577, 602, 1634, 1829, 2084, 2547
Eco53kI	GAGCTC	1 cut(s)	793
Eco57I	CTGAAG	1 cut(s)	1671
Eco72I	CACGTG	1 cut(s)	839
Eco81I	CCTNAGG	1 cut(s)	1928
EcoICRI	GAGCTC	1 cut(s)	793
EcoNI	CCTNNNNNAGG	1 cut(s)	548
EcoO109I	RGGNCCY	1 cut(s)	1634
EcoRII	CCWGG	8 cut(s)	28, 531, 598, 1049, 2063, 2098, 2289, 2523
EcoRV	GATATC	2 cut(s)	2185, 2338
EcoT14I	CCWWGG	2 cut(s)	1398, 1818
EcoT22I	ATGCAT	3 cut(s)	18, 1495, 2266
EcoT38I	GRGCYC	2 cut(s)	687, 795
EgeI	GGCGCC	1 cut(s)	2222
EheI	GGCGCC	1 cut(s)	2222
ErhI	CCWWGG	2 cut(s)	1398, 1818
FauI	CCCGC	3 cut(s)	38, 641, 763
FauNDI	CATATG	1 cut(s)	1525
FbaI	TGATCA	1 cut(s)	1030
FblI	GTMKAC	3 cut(s)	117, 570, 798
FokI	GGATG	4 cut(s)	500, 604, 2243, 2431
FriOI	GRGCYC	2 cut(s)	687, 795
FspBI	CTAG	5 cut(s)	680, 1158, 1518, 1628, 2357
GlaI	GCGC	3 cut(s)	6, 1894, 2222
GsaI	CCCAGC	3 cut(s)	141, 475, 503
GsuI	CTGGAG	3 cut(s)	251, 1072, 2253
HaeII	RGCGCY	2 cut(s)	8, 2224
HaeIII	GGCC	5 cut(s)	927, 937, 1859, 2298, 2461
HapII	CCGG	5 cut(s)	89, 558, 605, 2433, 2462
HgaI	GACGC	1 cut(s)	859
HhaI	GCGC	3 cut(s)	7, 1895, 2223
Hin1I	GRCGYC	1 cut(s)	2221
Hin6I	GCGC	3 cut(s)	5, 1893, 2221
HinP1I	GCGC	3 cut(s)	5, 1893, 2221
HincII	GTYRAC	3 cut(s)	571, 909, 2500
HindII	GTYRAC	3 cut(s)	571, 909, 2500
HpaI	GTTAAC	1 cut(s)	2500
HpaII	CCGG	5 cut(s)	89, 558, 605, 2433, 2462
HphI	GGTGA	3 cut(s)	1362, 2207, 2269
Hpy166II	GTNNAC	8 cut(s)	84, 118, 277, 571, 799, 909, 2277, 2500
Hpy8I	GTNNAC	8 cut(s)	84, 118, 277, 571, 799, 909, 2277, 2500
Hpy99I	CGWCG	1 cut(s)	626
HpyAV	CCTTC	4 cut(s)	244, 1960, 2070, 2105
HpyCH4III	ACNGT	4 cut(s)	274, 1268, 1594, 1689
HpyCH4IV	ACGT	3 cut(s)	75, 838, 2010
HpySE526I	ACGT	3 cut(s)	75, 838, 2010
Hsp92I	GRCGYC	1 cut(s)	2221
HspAI	GCGC	3 cut(s)	5, 1893, 2221
KasI	GGCGCC	1 cut(s)	2220
Kpn2I	TCCGGA	1 cut(s)	604
Ksp22I	TGATCA	1 cut(s)	1030
KspAI	GTTAAC	1 cut(s)	2500
Kzo9I	GATC	6 cut(s)	204, 712, 1030, 1813, 2102, 2151
LmnI	GCTCC	7 cut(s)	91, 270, 1016, 1053, 1456, 1597, 1780
MaeI	CTAG	5 cut(s)	680, 1158, 1518, 1628, 2357
MaeII	ACGT	3 cut(s)	75, 838, 2010
MaeIII	GTNAC	4 cut(s)	839, 1074, 1303, 1919
MalI	GATC	6 cut(s)	206, 714, 1032, 1815, 2104, 2153
MbiI	CCGCTC	1 cut(s)	1464
MboI	GATC	6 cut(s)	204, 712, 1030, 1813, 2102, 2151
MboII	GAAGA	9 cut(s)	249, 326, 500, 536, 887, 906, 1310, 1664, 1732
MfeI	CAATTG	1 cut(s)	786
MflI	RGATCY	3 cut(s)	1813, 2102, 2151
MhlI	GDGCHC	7 cut(s)	131, 176, 687, 795, 1461, 1732, 1891
MluCI	AATT	3 cut(s)	786, 1167, 1955
Mly113I	GGCGCC	1 cut(s)	2221
MlyI	GAGTC	4 cut(s)	17, 123, 431, 1163
MmeI	TCCRAC	4 cut(s)	87, 210, 1015, 1250
Mph1103I	ATGCAT	3 cut(s)	18, 1495, 2266
MroI	TCCGGA	1 cut(s)	604
MseI	TTAA	8 cut(s)	308, 755, 761, 1236, 1248, 1317, 1899, 2499
MslI	CAYNNNNRTG	1 cut(s)	2229
MspA1I	CMGCKG	3 cut(s)	42, 247, 503
MspI	CCGG	5 cut(s)	89, 558, 605, 2433, 2462
MspR9I	CCNGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
MunI	CAATTG	1 cut(s)	786
Mva1269I	GAATGC	2 cut(s)	1640, 1717
MvaI	CCWGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
MvnI	CGCG	2 cut(s)	732, 1893
NarI	GGCGCC	1 cut(s)	2221
NdeI	CATATG	1 cut(s)	1525
NdeII	GATC	6 cut(s)	204, 712, 1030, 1813, 2102, 2151
NmeAIII	GCCGAG	2 cut(s)	241, 260
NmuCI	GTSAC	2 cut(s)	839, 1074
NruI	TCGCGA	1 cut(s)	732
NsiI	ATGCAT	3 cut(s)	18, 1495, 2266
NspI	RCATGY	3 cut(s)	805, 1509, 2308
PagI	TCATGA	2 cut(s)	432, 2538
PaqCI	CACCTGC	1 cut(s)	85
PcsI	WCGNNNNNNNCGW	2 cut(s)	736, 1749
PctI	GAATGC	2 cut(s)	1640, 1717
PfeI	GAWTC	7 cut(s)	62, 254, 608, 1085, 1757, 2353, 2510
PflFI	GACNNNGTC	1 cut(s)	575
PfoI	TCCNGGA	1 cut(s)	2098
PleI	GAGTC	4 cut(s)	17, 122, 430, 1162
PluTI	GGCGCC	1 cut(s)	2224
PmaCI	CACGTG	1 cut(s)	839
PmlI	CACGTG	1 cut(s)	839
PpsI	GAGTC	4 cut(s)	17, 122, 430, 1162
Ppu21I	YACGTR	2 cut(s)	839, 2011
PpuMI	RGGWCCY	1 cut(s)	1634
PshAI	GACNNNNGTC	1 cut(s)	882
Psp124BI	GAGCTC	1 cut(s)	795
Psp5II	RGGWCCY	1 cut(s)	1634
Psp6I	CCWGG	8 cut(s)	28, 531, 598, 1049, 2063, 2098, 2289, 2523
PspCI	CACGTG	1 cut(s)	839
PspFI	CCCAGC	3 cut(s)	137, 471, 499
PspGI	CCWGG	8 cut(s)	28, 531, 598, 1049, 2063, 2098, 2289, 2523
PspPI	GGNCC	8 cut(s)	577, 602, 926, 935, 1634, 1829, 2084, 2547
PspPPI	RGGWCCY	1 cut(s)	1634
PsrI	GAACNNNNNNTAC	2 cut(s)	2016, 2048
PstI	CTGCAG	2 cut(s)	644, 1419
PstNI	CAGNNNCTG	2 cut(s)	382, 1222
PsuI	RGATCY	3 cut(s)	1813, 2102, 2151
PsyI	GACNNNGTC	1 cut(s)	575
PvuII	CAGCTG	1 cut(s)	503
RruI	TCGCGA	1 cut(s)	732
RsaI	GTAC	4 cut(s)	74, 83, 1679, 2009
RsaNI	GTAC	4 cut(s)	73, 82, 1678, 2008
RseI	CAYNNNNRTG	1 cut(s)	2229
SacI	GAGCTC	1 cut(s)	795
SalI	GTCGAC	1 cut(s)	569
SaqAI	TTAA	8 cut(s)	308, 755, 761, 1236, 1248, 1317, 1899, 2499
Sau3AI	GATC	6 cut(s)	204, 712, 1030, 1813, 2102, 2151
Sau96I	GGNCC	8 cut(s)	577, 602, 926, 935, 1634, 1829, 2084, 2547
SchI	GAGTC	4 cut(s)	17, 123, 431, 1163
ScrFI	CCNGG	8 cut(s)	30, 533, 600, 1051, 2065, 2100, 2291, 2525
SduI	GDGCHC	7 cut(s)	131, 176, 687, 795, 1461, 1732, 1891
SfcI	CTRYAG	3 cut(s)	640, 1415, 2146
SfoI	GGCGCC	1 cut(s)	2222
SinI	GGWCC	6 cut(s)	577, 602, 1634, 1829, 2084, 2547
SmiMI	CAYNNNNRTG	1 cut(s)	2229
SmlI	CTYRAG	2 cut(s)	746, 1149
SmoI	CTYRAG	2 cut(s)	746, 1149
Sse9I	AATT	3 cut(s)	786, 1167, 1955
SspDI	GGCGCC	1 cut(s)	2220
SspI	AATATT	1 cut(s)	1573
SspMI	CTAG	5 cut(s)	680, 1158, 1518, 1628, 2357
SstI	GAGCTC	1 cut(s)	795
StyD4I	CCNGG	8 cut(s)	28, 531, 598, 1049, 2063, 2098, 2289, 2523
StyI	CCWWGG	2 cut(s)	1398, 1818
TaaI	ACNGT	4 cut(s)	274, 1268, 1594, 1689
TaiI	ACGT	3 cut(s)	78, 841, 2013
TaqI	TCGA	6 cut(s)	19, 65, 570, 1752, 2181, 2310
TasI	AATT	3 cut(s)	786, 1167, 1955
TatI	WGTACW	1 cut(s)	1677
TauI	GCSGC	3 cut(s)	45, 836, 1505
TfiI	GAWTC	7 cut(s)	62, 254, 608, 1085, 1757, 2353, 2510
Tru1I	TTAA	8 cut(s)	308, 755, 761, 1236, 1248, 1317, 1899, 2499
Tru9I	TTAA	8 cut(s)	308, 755, 761, 1236, 1248, 1317, 1899, 2499
TscAI	CASTG	9 cut(s)	279, 649, 1234, 1702, 1888, 1941, 2164, 2277, 2525
TseFI	GTSAC	2 cut(s)	839, 1074
Tsp45I	GTSAC	2 cut(s)	839, 1074
TspDTI	ATGAA	8 cut(s)	311, 386, 608, 1098, 1770, 1787, 2527, 2555
TspGWI	ACGGA	2 cut(s)	378, 2386
TspRI	CASTG	9 cut(s)	279, 649, 1234, 1702, 1888, 1941, 2164, 2277, 2525
Tth111I	GACNNNGTC	1 cut(s)	575
VpaK11BI	GGWCC	6 cut(s)	577, 602, 1634, 1829, 2084, 2547
XagI	CCTNNNNNAGG	1 cut(s)	548
XapI	RAATTY	1 cut(s)	1167
XbaI	TCTAGA	1 cut(s)	2356
XceI	RCATGY	3 cut(s)	805, 1509, 2308
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2521
XmiI	GTMKAC	3 cut(s)	117, 570, 798
XspI	CTAG	5 cut(s)	680, 1158, 1518, 1628, 2357
Zsp2I	ATGCAT	3 cut(s)	18, 1495, 2266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.