MD10G1256000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013297
Physical Location & Seq
Forward (+)
35051249 .. 35053124
1876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1256000.v1.1.491

Sequence Viewer

Length: 930 bp
ATGAACCCGATTCGAGCATTTTCATCTCCTGCAGAGTTGCCAGCTTCTTCCACAGCTTCTAAAGAACGCCCAGAAGATCATAGTTTTGATGGCATTGCCACCAATGTGAAGTTATTATTAAAGCTAATCCAAGAGCAAAATGAGGCCTGCACAAAAGACAATGATGACAGGAAGATGCAAAGGGTGGCGGGAATGATGACGATAATTGATGATGTTAAAACCCGGATTCAGAAATCTCAAACCGTTAAGAGGAGAGCAGAGCTCCGGCGATGCAATACAGATCTTAGGCCAAATGTTCCAAGGGACAAAAGGCCTCCGGAGCCAGTAATTGATGAGAAAGAAAGGTTGAGAAGACAGCTTAGCGCAAGCTTGGCAGCGCGAAAGAGCCTAGAAATAATGTGTTCAGGCTTGGGAAAGGAGAACGAGATTATTGCATCGGAGCTTGCGAGAAAGGTTCATGAATTGACTGGGATGGAGGAACATATCAATGATCTTCGAGCGCAAAATGAGATGCTGTTGGCAAAAGTACAAGCTTGTGCAGCAGAGCACAAGGAGAAGAGGTGTGCCGGTGGTGGGGTAGAGATTCATGGGAATGCAGCACTCCAGGAGCGAAACAAGGCACTTTCGGAGCAACTTCTTAAGTCCCTTGATGGGTGTAGATCCTTAAAGAGAAAGATCAAAGACGCACAAAAGGAAAACAATGGGATGCACTCGACCATGGAGGAGATGGGGGTGGAAGTTCAAGAAGGCCTAGAACGAATACGCGCACTCAAGCAAAAGATTGCCACAAGCAATGAGCAAGCAGCAGATATTGAAGAGGAGATCTCAGCATTGGAGCATCTGTTTGAGAGCTTCAGTATGAAGATTTCGAAGCATGGGTCGAAGAAAGGTGAAAGTGCAAAACACAACACTGAGATCGACACTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

34.65

Weight (kDa)

8.14

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03620 AT4G03620
fragaria_vesca FvH4_3g09520 FvH4_3g09520
malus_domestica MD05G1060100.v1.1 MD10G1256000.v1.1
prunus_persica Prupe.4G087600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0015101
rosa_laevigata RLG00000032170
rosa_multiflora Rmu_sc0002209.1_g000004
rosa_roxburghii Rroxscaffold_1G00061430
rosa_rugosa Rorug05G0024300 Rorug05G0024300
rosa_samantha Rh5AG118700 Rh5BG116600 Rh5CG127700 Rh5DG115600
rosa_wichuraiana Rw5G010270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 379, 765
AccIII TCCGGA 1 cut(s) 316
AciI CCGC 1 cut(s) 188
AclWI GGATC 1 cut(s) 654
AcuI CTGAAG 1 cut(s) 838
AfaI GTAC 1 cut(s) 528
AfiI CCNNNNNNNGG 3 cut(s) 249, 573, 651
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 2 cut(s) 743, 815
AjnI CCWGG 1 cut(s) 603
AleI CACNNNNGTG 1 cut(s) 104
AluBI AGCT 9 cut(s) 44, 56, 124, 262, 358, 369, 442, 533, 852
AluI AGCT 9 cut(s) 44, 56, 124, 262, 358, 369, 442, 533, 852
Alw21I GWGCWC 2 cut(s) 264, 549
AlwI GGATC 1 cut(s) 654
Aor13HI TCCGGA 1 cut(s) 316
AoxI GGCC 4 cut(s) 144, 287, 311, 748
ApeKI GCWGC 4 cut(s) 374, 539, 596, 803
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
AspLEI GCGC 4 cut(s) 365, 379, 502, 767
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 902
AsuII TTCGAA 1 cut(s) 869
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 358
Bbv12I GWGCWC 2 cut(s) 264, 549
BbvI GCAGC 4 cut(s) 386, 551, 608, 815
BccI CCATC 4 cut(s) 83, 466, 644, 721
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 1 cut(s) 605
BcnI CCSGG 1 cut(s) 223
BfaI CTAG 2 cut(s) 389, 752
BfmI CTRYAG 1 cut(s) 30
BfrI CTTAAG 1 cut(s) 638
BglII AGATCT 2 cut(s) 280, 822
BisI GCNGC 4 cut(s) 375, 540, 597, 804
BlpI GCTNAGC 1 cut(s) 359
BlsI GCNGC 4 cut(s) 376, 541, 598, 805
Bme1390I CCNGG 2 cut(s) 223, 605
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 2 cut(s) 223, 605
BmrI ACTGGG 1 cut(s) 477
BmsI GCATC 6 cut(s) 165, 260, 443, 501, 696, 847
BmuI ACTGGG 1 cut(s) 477
BpiI GAAGAC 1 cut(s) 358
BplI GAGNNNNNCTC 4 cut(s) 246, 278, 809, 841
BpmI CTGGAG 1 cut(s) 587
Bpu1102I GCTNAGC 1 cut(s) 359
Bpu14I TTCGAA 1 cut(s) 869
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 299, 717
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 4 cut(s) 545, 575, 827, 857
Bsc4I CCNNNNNNNGG 3 cut(s) 249, 573, 651
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 2 cut(s) 323, 472
Bse3DI GCAATG 2 cut(s) 93, 799
BseAI TCCGGA 1 cut(s) 316
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 299, 717
BseGI GGATG 2 cut(s) 477, 711
BseLI CCNNNNNNNGG 3 cut(s) 249, 573, 651
BseMI GCAATG 2 cut(s) 93, 799
BseMII CTCAG 2 cut(s) 840, 903
BseNI ACTGG 2 cut(s) 323, 472
BseRI GAGGAG 3 cut(s) 265, 737, 833
BseXI GCAGC 4 cut(s) 386, 551, 608, 815
BsgI GTGCAG 2 cut(s) 133, 558
Bsh1236I CGCG 2 cut(s) 379, 765
BshFI GGCC 4 cut(s) 146, 289, 313, 750
BsiHKAI GWGCWC 2 cut(s) 264, 549
BsiSI CCGG 4 cut(s) 223, 265, 317, 567
BslFI GGGAC 2 cut(s) 317, 628
BslI CCNNNNNNNGG 3 cut(s) 249, 573, 651
BsmFI GGGAC 2 cut(s) 317, 628
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 4 cut(s) 146, 289, 313, 750
Bsp119I TTCGAA 1 cut(s) 869
Bsp1286I GDGCHC 2 cut(s) 264, 549
Bsp13I TCCGGA 1 cut(s) 316
Bsp143I GATC 7 cut(s) 76, 280, 490, 659, 675, 822, 915
Bsp1720I GCTNAGC 1 cut(s) 359
Bsp19I CCATGG 1 cut(s) 717
BspACI CCGC 1 cut(s) 188
BspANI GGCC 4 cut(s) 146, 289, 313, 750
BspCNI CTCAG 2 cut(s) 839, 904
BspEI TCCGGA 1 cut(s) 316
BspFNI CGCG 2 cut(s) 379, 765
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 321
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 869
BspTI CTTAAG 1 cut(s) 638
BsrDI GCAATG 2 cut(s) 93, 799
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 2 cut(s) 323, 472
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 2 cut(s) 299, 717
BssMI GATC 7 cut(s) 76, 280, 490, 659, 675, 822, 915
BssT1I CCWWGG 2 cut(s) 299, 717
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 2 cut(s) 551, 810
BstAFI CTTAAG 1 cut(s) 638
BstBI TTCGAA 1 cut(s) 869
BstC8I GCNNGC 5 cut(s) 42, 148, 367, 444, 801
BstDEI CTNAG 4 cut(s) 284, 359, 826, 912
BstDSI CCRYGG 1 cut(s) 717
BstF5I GGATG 2 cut(s) 477, 711
BstFNI CGCG 2 cut(s) 379, 765
BstHHI GCGC 4 cut(s) 365, 379, 502, 767
BstKTI GATC 7 cut(s) 79, 283, 493, 662, 678, 825, 918
BstMBI GATC 7 cut(s) 76, 280, 490, 659, 675, 822, 915
BstMWI GCNNNNNNNGC 3 cut(s) 319, 371, 539
BstNI CCWGG 1 cut(s) 605
BstSCI CCNGG 2 cut(s) 221, 603
BstSFI CTRYAG 1 cut(s) 30
BstUI CGCG 2 cut(s) 379, 765
BstV1I GCAGC 4 cut(s) 386, 551, 608, 815
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 3 cut(s) 280, 659, 822
BstYI RGATCY 3 cut(s) 280, 659, 822
BsuRI GGCC 4 cut(s) 146, 289, 313, 750
BtgI CCRYGG 1 cut(s) 717
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 2 cut(s) 477, 711
BtsIMutI CAGTG 1 cut(s) 909
Cac8I GCNNGC 5 cut(s) 42, 148, 367, 444, 801
CciI TCATGA 1 cut(s) 457
CfoI GCGC 4 cut(s) 365, 379, 502, 767
Cfr10I RCCGGY 1 cut(s) 566
CseI GACGC 1 cut(s) 692
Csp6I GTAC 1 cut(s) 527
CviAII CATG 4 cut(s) 458, 587, 718, 875
CviQI GTAC 1 cut(s) 527
DdeI CTNAG 4 cut(s) 284, 359, 826, 912
DpnI GATC 7 cut(s) 78, 282, 492, 661, 677, 824, 917
DpnII GATC 7 cut(s) 76, 280, 490, 659, 675, 822, 915
Eam1104I CTCTTC 2 cut(s) 551, 810
EarI CTCTTC 2 cut(s) 551, 810
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 2 cut(s) 299, 717
Eco147I AGGCCT 3 cut(s) 146, 313, 750
Eco24I GRGCYC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 838
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 2 cut(s) 299, 717
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 2 cut(s) 299, 717
FaeI CATG 4 cut(s) 461, 590, 721, 878
FaiI YATR 7 cut(s) 81, 459, 483, 588, 719, 860, 876
FaqI GGGAC 2 cut(s) 317, 628
FatI CATG 4 cut(s) 457, 586, 717, 874
FauI CCCGC 1 cut(s) 181
Fnu4HI GCNGC 4 cut(s) 375, 540, 597, 804
FokI GGATG 2 cut(s) 484, 718
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 4 cut(s) 375, 540, 597, 804
FspBI CTAG 2 cut(s) 389, 752
GlaI GCGC 4 cut(s) 364, 378, 501, 766
GluI GCNGC 4 cut(s) 375, 540, 597, 804
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 4 cut(s) 146, 289, 313, 750
HapII CCGG 4 cut(s) 223, 265, 317, 567
HgaI GACGC 1 cut(s) 692
HhaI GCGC 4 cut(s) 365, 379, 502, 767
Hin1II CATG 4 cut(s) 461, 590, 721, 878
Hin6I GCGC 4 cut(s) 363, 377, 500, 765
HinP1I GCGC 4 cut(s) 363, 377, 500, 765
HindIII AAGCTT 2 cut(s) 367, 531
HinfI GANTC 3 cut(s) 10, 226, 583
HpaII CCGG 4 cut(s) 223, 265, 317, 567
HphI GGTGA 1 cut(s) 902
Hpy188I TCNGA 3 cut(s) 231, 439, 628
Hpy188III TCNNGA 3 cut(s) 317, 458, 743
HpyAV CCTTC 1 cut(s) 740
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 9 cut(s) 32, 150, 178, 273, 434, 539, 596, 709, 899
HpyF10VI GCNNNNNNNGC 3 cut(s) 319, 371, 539
HpyF3I CTNAG 4 cut(s) 284, 359, 826, 912
Hsp92II CATG 4 cut(s) 461, 590, 721, 878
HspAI GCGC 4 cut(s) 363, 377, 500, 765
Kpn2I TCCGGA 1 cut(s) 316
Kzo9I GATC 7 cut(s) 76, 280, 490, 659, 675, 822, 915
LmnI GCTCC 6 cut(s) 267, 319, 439, 607, 628, 835
Lsp1109I GCAGC 4 cut(s) 386, 551, 608, 815
LweI GCATC 6 cut(s) 165, 260, 443, 501, 696, 847
MaeI CTAG 2 cut(s) 389, 752
MalI GATC 7 cut(s) 78, 282, 492, 661, 677, 824, 917
MboI GATC 7 cut(s) 76, 280, 490, 659, 675, 822, 915
MboII GAAGA 9 cut(s) 39, 86, 184, 363, 485, 568, 827, 874, 895
MflI RGATCY 3 cut(s) 280, 659, 822
MhlI GDGCHC 2 cut(s) 264, 549
MluCI AATT 3 cut(s) 204, 327, 461
MnlI CCTC 7 cut(s) 136, 243, 324, 469, 552, 715, 811
MroI TCCGGA 1 cut(s) 316
MseI TTAA 6 cut(s) 119, 216, 246, 639, 665, 928
MslI CAYNNNNRTG 3 cut(s) 104, 486, 591
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 4 cut(s) 223, 265, 317, 567
MspR9I CCNGG 2 cut(s) 223, 605
Mva1269I GAATGC 1 cut(s) 598
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 2 cut(s) 379, 765
MwoI GCNNNNNNNGC 3 cut(s) 319, 371, 539
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 1 cut(s) 717
NdeII GATC 7 cut(s) 76, 280, 490, 659, 675, 822, 915
NlaIII CATG 4 cut(s) 461, 590, 721, 878
NlaIV GGNNCC 1 cut(s) 321
NspV TTCGAA 1 cut(s) 869
OliI CACNNNNGTG 1 cut(s) 104
PagI TCATGA 1 cut(s) 457
PceI AGGCCT 3 cut(s) 146, 313, 750
PcsI WCGNNNNNNNCGW 1 cut(s) 443
PctI GAATGC 1 cut(s) 598
PfeI GAWTC 3 cut(s) 10, 226, 583
PfoI TCCNGGA 1 cut(s) 603
PkrI GCNGC 4 cut(s) 376, 541, 598, 805
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 321
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 3 cut(s) 280, 659, 822
RsaI GTAC 1 cut(s) 528
RsaNI GTAC 1 cut(s) 527
RseI CAYNNNNRTG 3 cut(s) 104, 486, 591
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 6 cut(s) 119, 216, 246, 639, 665, 928
SatI GCNGC 4 cut(s) 375, 540, 597, 804
Sau3AI GATC 7 cut(s) 76, 280, 490, 659, 675, 822, 915
ScrFI CCNGG 2 cut(s) 223, 605
SduI GDGCHC 2 cut(s) 264, 549
SfaNI GCATC 6 cut(s) 165, 260, 443, 501, 696, 847
SfcI CTRYAG 1 cut(s) 30
SfuI TTCGAA 1 cut(s) 869
SmiMI CAYNNNNRTG 3 cut(s) 104, 486, 591
SmlI CTYRAG 2 cut(s) 638, 770
SmoI CTYRAG 2 cut(s) 638, 770
Sse9I AATT 3 cut(s) 204, 327, 461
SseBI AGGCCT 3 cut(s) 146, 313, 750
SsiI CCGC 1 cut(s) 188
SspMI CTAG 2 cut(s) 389, 752
SstI GAGCTC 1 cut(s) 264
StuI AGGCCT 3 cut(s) 146, 313, 750
StyD4I CCNGG 2 cut(s) 221, 603
StyI CCWWGG 2 cut(s) 299, 717
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 6 cut(s) 13, 496, 713, 869, 881, 918
TasI AATT 3 cut(s) 204, 327, 461
TatI WGTACW 1 cut(s) 526
TfiI GAWTC 3 cut(s) 10, 226, 583
Tru1I TTAA 6 cut(s) 119, 216, 246, 639, 665, 928
Tru9I TTAA 6 cut(s) 119, 216, 246, 639, 665, 928
TscAI CASTG 1 cut(s) 916
TseI GCWGC 4 cut(s) 374, 539, 596, 803
TspDTI ATGAA 6 cut(s) 12, 17, 446, 474, 575, 875
TspRI CASTG 1 cut(s) 916
Vha464I CTTAAG 1 cut(s) 638
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XspI CTAG 2 cut(s) 389, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.