Rorug05G0024300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013297
Physical Location & Seq
Forward (+)
1543376 .. 1550030
6655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0024300.1

Sequence Viewer

Length: 3057 bp
ATGGAGTTCGAGAAGCGAAAAGCGGCGACGCTGACGTCACTGAGATCGGAGGTGACGGACAAGTCGCCGAAGGGCACGGTGGACACTCCGATCATTCCTCTCCTCGACGTCATCAACGGCCACCGCGACTACTTCACCACCAGCTCCTGCTCCGGCCGAATCTCCATCCTCTCTCAGCCCGCTCGCCGGGTCTCCAAGAAGAAAGCCTCCGGCGGCGCGTGGCTTTACATCTCTCACGACCCGGCCGATCCCGACTCCGTCCTCGACCTCGTCTTCTGCACCTCCGATCAGAATGAGCCGGAGCTCCAAGACGACCAAAACGACGTCGTTTTCAGATTCGAGCCTCTGATTATCGCCGTCGAGTGCAGGGACGTGGCGGCGGCGCAGGTTCTGGTGTCGAAAGCCATAGCGTCCGGGTTCAGAGAGTCCGGCATTACGAGTTCGAACAAGCGTGTGATTATCGCGATTCGCTGCTCGATACGATTGGAGGTTCCGCTGGGGAGTAGTCGCGAGATTATGGTGTCTCGCGAGTATTTGAGGTTCCTTGTGGGCGTGGCGAACGAGAAATTCGAGGCCAACCGGAAGAGGACCGAGGCTTTTCTGGAGGCTCTGCAGAGTGGGGAGAGCGGAGGAGGGTTTGCGCTGGCTCCGGCTGGTGGAAATTACGAGAATGCCTCTTCCGGGTTGAAGGGAGATAATGGATGCTCTAGTCTGTCTGTTGTTCCGACAGAGGTTTCCGGGGAGAATGAGGAGAATCTTTACCTCTGGGGTCATTCGGCTTGCGCGTTGGAGAAGAAGGTGGATAATGGGATTCTTGTGTTTGGTGGATTCGGAGGCATTGGAAGACATGCGAGGAGAAATCAGTGTTTGCTGGTTGATGCATTGTCTGGTAGTTTGAGAGGGATTAGCATAGAGAGTAGTCCTTCACCGAGATTGGGCCACACGGCTTCTTTGGTTGGACATTGTGTGTTTGTTATTGGAGGCAGGGCTGATCCTGAGAAAATTCTGAGTGATGTATGGGTCCTCGATATACAGAAGAAGGAATGGAAACTCGCCGAATGTTCTGGTGATGTATTTCCTCCCAGGCATCGACATGCTGCAGCCGTGGTAGGATCAAAGATATATGTGTTTGGTGGACTTAACAATGATGCAGTCACATCTTCCTTGCATGTACTGGATACAGATAATCTGCAGTGGAGAGAGATAGTTGTCACTGGGGAAGGTCCGTGTGCCCGACATTCACACTCAATGGTGCCATCGGGATCTCAGCTATATATGTTTGGAGGATATGATGGTGAGGAAGCACTTGGAGACTTGTACAGGTTTGATACTGAAAAATCTAACTGTAGATGGAAAAAAGTGAAGGCGTCTGGGAGAAGTCCCCATGCAAGGTTCTCGCACTCCATGTTTGTGTACAAAAATCATCTTGGAGTTATTGGTGGTTGTCCAGTTAGACAACATTGCCAAGAGTTGGCAATACTTGATCTACGGCTGTGCATGTGGAGGCATGTGAAACTAGAATCCACTGGTGAAGATTTGTTTGTACGTAGCACTGCCAATGTTGTTGGTGATGATCTTGTTATGATTGGTGGTGGGGCATCTTGTTATGCATTTGGTACAAAGTTCAGTAAGCCAATGAAAATCAACTTGCTTCCCTTGAAGCTTAGAGATGATAAACCACTTGTTAGAGAACGGCATACTGACCAAATTGATATCGTGAGGAGCGAAAAGAGTAGACATCCACAGGTTCAAAATGTTAAAACATTAACCGAAGCTCCTGACCTAAATTTTAAGAGTGAATTACCCATGGCTGATGGCATTGGTCAACAGGTGGATGCATATTGGGTTCTGAAACTTGAAAGAAAGTATGCGAAAATAGGGAAGGACATACTAAAGAAGTTTGGTTGGTTAGATCTTGCAAGGAAGGTTTACTCCTTGGAAGGTGGTTTACATATTTGTTTCCCTGTGAGTGCAAAATTTTCTGATGTATTGAAAGAAAATCAGCATCATATGGCAAATTCATTTGAAGGACAGAGTGGTCACATTTGTAAGCCAGATATAGGAAGGGAATGTTTGATTGATGAGGTCACCTGTTCAAAAGCCTTAGACATTCTAAAGGAATGTGGTGCCATGAAGCTGGTAGATGAGGTTGTTGAAGTCAGAAAAACTGCAAAATCTCCCTTGAAAATAATGAGTGATGCCGTGGGGTCTCTGCTAGAGGATAAAGGCCTACCTGCAGGACTGTTGGAGGAGCTACCAACCAGATGGGAGCGACTTGGAGATATTGTTGTGCTTCCAGTGACATCATTCAAGAATCCGTTATGGGACTCAATTGCTGAGGAGCTTTGGCCTGCTGTTGCCAAATCAGTAAATGCTGTTCGCCTTGCTCGCCAAGGCCGAGTTGCATCAACTGGTACAAGGGACAGTACTTTGGAGATTCTTATAGGAGATAATGGCTGGGTTGATCATCGTGAAAATGGAATTCTCTATTCTTTTGATGCTACTAAGTGCATGTTCTCCTGGGGCAACCTTTCTGAGAAGCTCCGCATGGGCTCTCTGGATTGTAGAGATGAGATCATTGTGGATTTATTTGCTGGAATTGGATATTTTATGCTGCCATTTCTTGTCAGGGCCAAGGCAAAACTAGTTTATGCTTGTGAATGGAATCCTCATGCTGTTGCGGCACTCCGGAGAAACGTACAAGCCAATTCTGTCAGTGATCGATGCATCATTCTTGAAGGAGATAACCGGACAACGGCACCTACAGGAGTGGCTGATCGGGTCTGCCTTGGTCTCATTCCGTCAAGTGAATCTAGCTGGGCCACTGCTGTTAGGGCATTAAGGGATGAGGGTGGGATGCTACATGTGCATGGGAATGTGGTGGACTCAGAGGAGAATTCATGGACCAATCACGTAACAGAATCAATAGCTGAAACTGCTAGATCTCAAGGTCATTGTTGGGAGGTTTCAATAGAACATTTGGAGAGAGTGAAATGGTACGCCCCACACATCCGCCACGTTGTTGCAGATGTAAGATGTAGACAGACCCAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1018

Amino Acids

112.24

Weight (kDa)

6.7

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 5 - 205 6e-67 Methyltransferase TYW3
Beta-prop_FBX42 PF13415 255 - 349 2.6e-11 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 256 - 418 1.5e-35 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 270 - 430 1.7e-14 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 273 - 352 1.1e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 300 - 400 2.9e-11 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 304 - 381 3.4e-17 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 308 - 543 1.3e-26 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 310 - 350 5.1e-08 Kelch motif
Kelch_2 PF07646 310 - 351 2.8e-06 Kelch motif
Beta-prop_Calicin PF13964 322 - 448 7e-08 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 337 - 418 7.7e-12 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 361 - 401 3.2e-08 Kelch motif
Kelch_1 PF01344 361 - 402 4.3e-09 Kelch motif
Beta-prop_FBX42 PF13415 362 - 532 1.4e-27 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 365 - 535 1.6e-39 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 397 - 483 1.4e-07 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 398 - 447 1.5e-06 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 412 - 448 4.3e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 424 - 538 1.9e-13 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 741 - 818 1.5e-14 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 823 - 962 4e-32 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013297)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03620 AT4G03620
fragaria_vesca FvH4_3g09520 FvH4_3g09520
malus_domestica MD05G1060100.v1.1 MD10G1256000.v1.1
prunus_persica Prupe.4G087600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0015101
rosa_laevigata RLG00000032170
rosa_multiflora Rmu_sc0002209.1_g000004
rosa_roxburghii Rroxscaffold_1G00061430
rosa_rugosa Rorug05G0024300 Rorug05G0024300
rosa_samantha Rh5AG118700 Rh5BG116600 Rh5CG127700 Rh5DG115600
rosa_wichuraiana Rw5G010270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 34, 61, 2037
AatII GACGTC 3 cut(s) 38, 111, 327
Acc36I ACCTGC 2 cut(s) 376, 2242
AccB1I GGYRCC 3 cut(s) 1252, 2126, 2758
AccB7I CCANNNNNTGG 1 cut(s) 1471
AccBSI CCGCTC 2 cut(s) 182, 627
AccI GTMKAC 2 cut(s) 1735, 3040
AccII CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
AccIII TCCGGA 1 cut(s) 2688
AclWI GGATC 4 cut(s) 242, 986, 1120, 1270
AcoI YGGCCR 3 cut(s) 118, 154, 243
AcsI RAATTY 7 cut(s) 566, 1002, 1786, 1976, 2017, 2481, 2896
AcyI GRCGYC 4 cut(s) 35, 108, 324, 1367
AfaI GTAC 9 cut(s) 1173, 1319, 1415, 1545, 1618, 2416, 2428, 2700, 2999
AfiI CCNNNNNNNGG 8 cut(s) 186, 656, 681, 935, 1389, 1471, 2060, 2755
AflIII ACRYGT 1 cut(s) 2863
AhlI ACTAGT 1 cut(s) 2644
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 2 cut(s) 1082, 2519
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 5 cut(s) 196, 528, 1305, 2214, 2798
AlwI GGATC 4 cut(s) 242, 986, 1120, 1270
AlwNI CAGNNNCTG 2 cut(s) 147, 391
Aor13HI TCCGGA 1 cut(s) 2688
ApeKI GCWGC 4 cut(s) 471, 1097, 1100, 2614
ApoI RAATTY 7 cut(s) 566, 1002, 1786, 1976, 2017, 2481, 2896
AspLEI GCGC 4 cut(s) 218, 385, 643, 785
AspS9I GGNCC 7 cut(s) 588, 937, 1021, 1223, 2631, 2820, 2904
AsuC2I CCSGG 5 cut(s) 188, 242, 415, 682, 739
AsuHPI GGTGA 8 cut(s) 64, 127, 918, 1079, 1307, 1541, 1580, 2080
AsuII TTCGAA 1 cut(s) 443
AvaII GGWCC 4 cut(s) 588, 1021, 1223, 2904
BaeGI GKGCMC 2 cut(s) 77, 1234
BanI GGYRCC 3 cut(s) 1252, 2126, 2758
BanII GRGCYC 2 cut(s) 306, 2555
BarI GAAGNNNNNNTAC 2 cut(s) 1932, 1964
BbsI GAAGAC 2 cut(s) 265, 850
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 2337
BbvI GCAGC 4 cut(s) 458, 1084, 1112, 2601
BccI CCATC 6 cut(s) 173, 1264, 1286, 1344, 1808, 2259
BceAI ACGGC 8 cut(s) 133, 341, 960, 1088, 1505, 1709, 2186, 2772
BcgI CGANNNNNNTGC 2 cut(s) 1377, 1411
BciT130I CCWGG 2 cut(s) 1084, 2521
BciVI GTATCC 1 cut(s) 1171
BclI TGATCA 1 cut(s) 2464
BcnI CCSGG 5 cut(s) 188, 242, 415, 682, 739
BcoDI GTCTC 5 cut(s) 196, 528, 1305, 2214, 2798
BcuI ACTAGT 1 cut(s) 2644
BfaI CTAG 6 cut(s) 708, 1517, 2216, 2645, 2814, 2940
BfmI CTRYAG 6 cut(s) 611, 1098, 1190, 1345, 2235, 2763
BfuAI ACCTGC 2 cut(s) 376, 2242
BfuI GTATCC 1 cut(s) 1171
BglII AGATCT 2 cut(s) 1912, 2942
BisI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
BlsI GCNGC 9 cut(s) 25, 215, 379, 382, 473, 1099, 1102, 2616, 2683
BmcAI AGTACT 1 cut(s) 2428
Bme1390I CCNGG 7 cut(s) 188, 242, 415, 682, 739, 1084, 2521
Bme18I GGWCC 4 cut(s) 588, 1021, 1223, 2904
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 7 cut(s) 588, 937, 1021, 1223, 2631, 2820, 2904
BmiI GGNNCC 7 cut(s) 492, 542, 648, 1022, 1254, 2128, 2760
BmrFI CCNGG 7 cut(s) 188, 242, 415, 682, 739, 1084, 2521
BmrI ACTGGG 1 cut(s) 1224
BmuI ACTGGG 1 cut(s) 1224
BpiI GAAGAC 2 cut(s) 265, 850
BplI GAGNNNNNCTC 2 cut(s) 659, 691
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 2337
Bpu14I TTCGAA 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 2931
BpuMI CCSGG 5 cut(s) 188, 242, 415, 682, 739
Bsa29I ATCGAT 1 cut(s) 2722
BsaAI YACGTR 2 cut(s) 1547, 2914
BsaHI GRCGYC 4 cut(s) 35, 108, 324, 1367
BsaI GGTCTC 3 cut(s) 196, 2214, 2798
BsaWI WCCGGW 3 cut(s) 579, 2688, 2748
BsaXI ACNNNNNCTCC 4 cut(s) 1759, 1789, 2271, 2301
Bsc4I CCNNNNNNNGG 8 cut(s) 186, 656, 681, 935, 1389, 1471, 2060, 2755
Bse1I ACTGG 6 cut(s) 1179, 1219, 1448, 1531, 2297, 2416
Bse3DI GCAATG 1 cut(s) 1459
BseAI TCCGGA 1 cut(s) 2688
BseBI CCWGG 2 cut(s) 1084, 2521
BseCI ATCGAT 1 cut(s) 2722
BseGI GGATG 7 cut(s) 165, 707, 1738, 1840, 2851, 2862, 3009
BseLI CCNNNNNNNGG 8 cut(s) 186, 656, 681, 935, 1389, 1471, 2060, 2755
BseMI GCAATG 1 cut(s) 1459
BseMII CTCAG 8 cut(s) 32, 188, 987, 998, 1280, 2328, 2526, 2901
BseNI ACTGG 6 cut(s) 1179, 1219, 1448, 1531, 2297, 2416
BseRI GAGGAG 8 cut(s) 92, 645, 764, 868, 1735, 2264, 2354, 2906
BseSI GKGCMC 2 cut(s) 77, 1234
BseX3I CGGCCG 2 cut(s) 154, 243
BseXI GCAGC 4 cut(s) 458, 1084, 1112, 2601
BseYI CCCAGC 3 cut(s) 496, 2457, 2817
BsgI GTGCAG 2 cut(s) 262, 385
Bsh1236I CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
Bsh1285I CGRYCG 2 cut(s) 157, 246
BshNI GGYRCC 3 cut(s) 1252, 2126, 2758
BshVI ATCGAT 1 cut(s) 2722
BsiEI CGRYCG 2 cut(s) 157, 246
BsiHKAI GWGCWC 1 cut(s) 306
BslFI GGGAC 4 cut(s) 383, 1365, 2339, 2435
BslI CCNNNNNNNGG 8 cut(s) 186, 656, 681, 935, 1389, 1471, 2060, 2755
BsmAI GTCTC 5 cut(s) 196, 528, 1305, 2214, 2798
BsmFI GGGAC 4 cut(s) 383, 1365, 2339, 2435
BsmI GAATGC 1 cut(s) 676
Bso31I GGTCTC 3 cut(s) 196, 2214, 2798
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 4 cut(s) 77, 306, 1234, 2555
Bsp13I TCCGGA 1 cut(s) 2688
Bsp1407I TGTACA 2 cut(s) 1317, 1413
Bsp19I CCATGG 1 cut(s) 1806
Bsp68I TCGCGA 3 cut(s) 464, 510, 528
BspCNI CTCAG 8 cut(s) 33, 187, 988, 999, 1279, 2329, 2527, 2900
BspDI ATCGAT 1 cut(s) 2722
BspEI TCCGGA 1 cut(s) 2688
BspFNI CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
BspLI GGNNCC 7 cut(s) 492, 542, 648, 1022, 1254, 2128, 2760
BspMAI CTGCAG 4 cut(s) 615, 1102, 1194, 2239
BspMI ACCTGC 2 cut(s) 376, 2242
BspPI GGATC 4 cut(s) 242, 986, 1120, 1270
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 3 cut(s) 1252, 2126, 2758
BspTNI GGTCTC 3 cut(s) 196, 2214, 2798
BsrBI CCGCTC 2 cut(s) 182, 627
BsrDI GCAATG 1 cut(s) 1459
BsrGI TGTACA 2 cut(s) 1317, 1413
BsrI ACTGG 6 cut(s) 1179, 1219, 1448, 1531, 2297, 2416
BssNI GRCGYC 4 cut(s) 35, 108, 324, 1367
BssT1I CCWWGG 5 cut(s) 1806, 1935, 2392, 2634, 2788
Bst2UI CCWGG 2 cut(s) 1084, 2521
Bst4CI ACNGT 4 cut(s) 79, 1346, 2244, 2426
Bst6I CTCTTC 2 cut(s) 578, 682
BstACI GRCGYC 4 cut(s) 35, 108, 324, 1367
BstAUI TGTACA 2 cut(s) 1317, 1413
BstBAI YACGTR 2 cut(s) 1547, 2914
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 6 cut(s) 180, 184, 645, 781, 2352, 2389
BstDSI CCRYGG 3 cut(s) 1104, 1806, 2202
BstEII GGTNACC 1 cut(s) 2086
BstF5I GGATG 7 cut(s) 165, 707, 1738, 1840, 2851, 2862, 3009
BstFNI CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
BstHHI GCGC 4 cut(s) 218, 385, 643, 785
BstMAI GTCTC 5 cut(s) 196, 528, 1305, 2214, 2798
BstMCI CGRYCG 2 cut(s) 157, 246
BstMWI GCNNNNNNNGC 5 cut(s) 2388, 2681, 2834, 2866, 2936
BstNI CCWGG 2 cut(s) 1084, 2521
BstNSI RCATGY 7 cut(s) 851, 1097, 1172, 1501, 1511, 2515, 2867
BstPI GGTNACC 1 cut(s) 2086
BstSCI CCNGG 7 cut(s) 186, 240, 413, 680, 737, 1082, 2519
BstSFI CTRYAG 6 cut(s) 611, 1098, 1190, 1345, 2235, 2763
BstSLI GKGCMC 2 cut(s) 77, 1234
BstSNI TACGTA 1 cut(s) 1547
BstUI CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
BstV1I GCAGC 4 cut(s) 458, 1084, 1112, 2601
BstV2I GAAGAC 2 cut(s) 265, 850
BstX2I RGATCY 3 cut(s) 1262, 1912, 2942
BstXI CCANNNNNNTGG 2 cut(s) 2137, 2265
BstYI RGATCY 3 cut(s) 1262, 1912, 2942
BstZI CGGCCG 2 cut(s) 154, 243
Bsu15I ATCGAT 1 cut(s) 2722
BsuI GTATCC 1 cut(s) 1171
BsuTUI ATCGAT 1 cut(s) 2722
BtgI CCRYGG 3 cut(s) 1104, 1806, 2202
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 7 cut(s) 165, 707, 1738, 1840, 2851, 2862, 3009
BtsI GCAGTG 3 cut(s) 1199, 1551, 2823
BtsIMutI CAGTG 9 cut(s) 38, 869, 1199, 1212, 1524, 1551, 2304, 2722, 2823
BtuMI TCGCGA 3 cut(s) 464, 510, 528
BveI ACCTGC 2 cut(s) 376, 2242
Cac8I GCNNGC 6 cut(s) 180, 184, 645, 781, 2352, 2389
CaiI CAGNNNCTG 2 cut(s) 147, 391
CfoI GCGC 4 cut(s) 218, 385, 643, 785
Cfr13I GGNCC 7 cut(s) 588, 937, 1021, 1223, 2631, 2820, 2904
ClaI ATCGAT 1 cut(s) 2722
CseI GACGC 3 cut(s) 37, 399, 1356
Csp6I GTAC 9 cut(s) 1172, 1318, 1414, 1544, 1617, 2415, 2427, 2699, 2998
CviQI GTAC 9 cut(s) 1172, 1318, 1414, 1544, 1617, 2415, 2427, 2699, 2998
DrdI GACNNNNNNGTC 3 cut(s) 34, 61, 2037
DseDI GACNNNNNNGTC 3 cut(s) 34, 61, 2037
EaeI YGGCCR 3 cut(s) 118, 154, 243
EagI CGGCCG 2 cut(s) 154, 243
Eam1104I CTCTTC 2 cut(s) 578, 682
EarI CTCTTC 2 cut(s) 578, 682
EciI GGCGGA 1 cut(s) 3002
Ecl136II GAGCTC 1 cut(s) 304
EclXI CGGCCG 2 cut(s) 154, 243
Eco105I TACGTA 1 cut(s) 1547
Eco130I CCWWGG 5 cut(s) 1806, 1935, 2392, 2634, 2788
Eco147I AGGCCT 1 cut(s) 2229
Eco24I GRGCYC 2 cut(s) 306, 2555
Eco31I GGTCTC 3 cut(s) 196, 2214, 2798
Eco32I GATATC 1 cut(s) 1714
Eco47I GGWCC 4 cut(s) 588, 1021, 1223, 2904
Eco52I CGGCCG 2 cut(s) 154, 243
Eco53kI GAGCTC 1 cut(s) 304
Eco91I GGTNACC 1 cut(s) 2086
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 1021
EcoO65I GGTNACC 1 cut(s) 2086
EcoRI GAATTC 2 cut(s) 2481, 2896
EcoRII CCWGG 2 cut(s) 1082, 2519
EcoRV GATATC 1 cut(s) 1714
EcoT14I CCWWGG 5 cut(s) 1806, 1935, 2392, 2634, 2788
EcoT22I ATGCAT 4 cut(s) 883, 1612, 1840, 2729
EcoT38I GRGCYC 2 cut(s) 306, 2555
ErhI CCWWGG 5 cut(s) 1806, 1935, 2392, 2634, 2788
FaqI GGGAC 4 cut(s) 383, 1365, 2339, 2435
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 1 cut(s) 2010
FbaI TGATCA 1 cut(s) 2464
FblI GTMKAC 2 cut(s) 1735, 3040
Fnu4HI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
FokI GGATG 7 cut(s) 152, 714, 1725, 1847, 2858, 2869, 2996
FriOI GRGCYC 2 cut(s) 306, 2555
Fsp4HI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
FspBI CTAG 6 cut(s) 708, 1517, 2216, 2645, 2814, 2940
GlaI GCGC 4 cut(s) 217, 384, 642, 784
GluI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
GsaI CCCAGC 3 cut(s) 500, 2461, 2821
GsuI CTGGAG 1 cut(s) 623
HgaI GACGC 3 cut(s) 37, 399, 1356
HhaI GCGC 4 cut(s) 218, 385, 643, 785
Hin1I GRCGYC 4 cut(s) 35, 108, 324, 1367
Hin6I GCGC 4 cut(s) 216, 383, 641, 783
HinP1I GCGC 4 cut(s) 216, 383, 641, 783
HincII GTYRAC 1 cut(s) 1826
HindII GTYRAC 1 cut(s) 1826
HindIII AAGCTT 1 cut(s) 1661
HphI GGTGA 8 cut(s) 64, 127, 918, 1079, 1307, 1541, 1580, 2080
Hpy166II GTNNAC 9 cut(s) 82, 1136, 1414, 1736, 1826, 1930, 1949, 2884, 3041
Hpy8I GTNNAC 9 cut(s) 82, 1136, 1414, 1736, 1826, 1930, 1949, 2884, 3041
Hpy99I CGWCG 5 cut(s) 31, 110, 326, 329, 362
HpyCH4III ACNGT 4 cut(s) 79, 1346, 2244, 2426
HpyCH4IV ACGT 8 cut(s) 35, 108, 324, 372, 1546, 2697, 2913, 3018
HpyF10VI GCNNNNNNNGC 5 cut(s) 2388, 2681, 2834, 2866, 2936
HpySE526I ACGT 8 cut(s) 35, 108, 324, 372, 1546, 2697, 2913, 3018
Hsp92I GRCGYC 4 cut(s) 35, 108, 324, 1367
HspAI GCGC 4 cut(s) 216, 383, 641, 783
Kpn2I TCCGGA 1 cut(s) 2688
Ksp22I TGATCA 1 cut(s) 2464
Lsp1109I GCAGC 4 cut(s) 458, 1084, 1112, 2601
MaeI CTAG 6 cut(s) 708, 1517, 2216, 2645, 2814, 2940
MaeII ACGT 8 cut(s) 35, 108, 324, 372, 1546, 2697, 2913, 3018
MaeIII GTNAC 8 cut(s) 36, 52, 1153, 1210, 2039, 2086, 2299, 2914
MbiI CCGCTC 2 cut(s) 182, 627
MboII GAAGA 9 cut(s) 211, 265, 595, 669, 805, 855, 1048, 1152, 1544
MfeI CAATTG 1 cut(s) 2331
MflI RGATCY 3 cut(s) 1262, 1912, 2942
MhlI GDGCHC 4 cut(s) 77, 306, 1234, 2555
MlyI GAGTC 4 cut(s) 248, 434, 2321, 2879
MmeI TCCRAC 4 cut(s) 749, 768, 937, 2226
Mph1103I ATGCAT 4 cut(s) 883, 1612, 1840, 2729
MroI TCCGGA 1 cut(s) 2688
MseI TTAA 5 cut(s) 1140, 1758, 1766, 1791, 2840
MslI CAYNNNNRTG 4 cut(s) 1092, 1409, 2868, 2874
MspA1I CMGCKG 1 cut(s) 496
MspR9I CCNGG 7 cut(s) 188, 242, 415, 682, 739, 1084, 2521
MunI CAATTG 1 cut(s) 2331
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 2 cut(s) 1084, 2521
MvnI CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
MwoI GCNNNNNNNGC 5 cut(s) 2388, 2681, 2834, 2866, 2936
NciI CCSGG 5 cut(s) 188, 242, 415, 682, 739
NcoI CCATGG 1 cut(s) 1806
NdeI CATATG 1 cut(s) 2010
NlaIV GGNNCC 7 cut(s) 492, 542, 648, 1022, 1254, 2128, 2760
NmeAIII GCCGAG 1 cut(s) 2423
NmuCI GTSAC 7 cut(s) 36, 52, 1153, 1210, 2039, 2086, 2299
NruI TCGCGA 3 cut(s) 464, 510, 528
NsiI ATGCAT 4 cut(s) 883, 1612, 1840, 2729
NspI RCATGY 7 cut(s) 851, 1097, 1172, 1501, 1511, 2515, 2867
NspV TTCGAA 1 cut(s) 443
PceI AGGCCT 1 cut(s) 2229
PciI ACATGT 1 cut(s) 2863
PcsI WCGNNNNNNNCGW 7 cut(s) 123, 243, 318, 567, 782, 1722, 2395
PctI GAATGC 1 cut(s) 676
PflFI GACNNNGTC 2 cut(s) 257, 269
PflMI CCANNNNNTGG 1 cut(s) 1471
PkrI GCNGC 9 cut(s) 25, 215, 379, 382, 473, 1099, 1102, 2616, 2683
PleI GAGTC 4 cut(s) 248, 433, 2321, 2879
PpsI GAGTC 4 cut(s) 248, 433, 2321, 2879
Ppu21I YACGTR 2 cut(s) 1547, 2914
PpuMI RGGWCCY 1 cut(s) 1021
PscI ACATGT 1 cut(s) 2863
Psp124BI GAGCTC 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 1021
Psp6I CCWGG 2 cut(s) 1082, 2519
PspEI GGTNACC 1 cut(s) 2086
PspFI CCCAGC 3 cut(s) 496, 2457, 2817
PspGI CCWGG 2 cut(s) 1082, 2519
PspN4I GGNNCC 7 cut(s) 492, 542, 648, 1022, 1254, 2128, 2760
PspPI GGNCC 7 cut(s) 588, 937, 1021, 1223, 2631, 2820, 2904
PspPPI RGGWCCY 1 cut(s) 1021
PsrI GAACNNNNNNTAC 4 cut(s) 524, 556, 2361, 2393
PstI CTGCAG 4 cut(s) 615, 1102, 1194, 2239
PstNI CAGNNNCTG 2 cut(s) 147, 391
PsuI RGATCY 3 cut(s) 1262, 1912, 2942
PsyI GACNNNGTC 2 cut(s) 257, 269
RruI TCGCGA 3 cut(s) 464, 510, 528
RsaI GTAC 9 cut(s) 1173, 1319, 1415, 1545, 1618, 2416, 2428, 2700, 2999
RsaNI GTAC 9 cut(s) 1172, 1318, 1414, 1544, 1617, 2415, 2427, 2699, 2998
RseI CAYNNNNRTG 4 cut(s) 1092, 1409, 2868, 2874
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 5 cut(s) 1140, 1758, 1766, 1791, 2840
SatI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
Sau96I GGNCC 7 cut(s) 588, 937, 1021, 1223, 2631, 2820, 2904
SbfI CCTGCAGG 1 cut(s) 2239
ScaI AGTACT 1 cut(s) 2428
SchI GAGTC 4 cut(s) 248, 434, 2321, 2879
ScrFI CCNGG 7 cut(s) 188, 242, 415, 682, 739, 1084, 2521
SdaI CCTGCAGG 1 cut(s) 2239
SduI GDGCHC 4 cut(s) 77, 306, 1234, 2555
SfcI CTRYAG 6 cut(s) 611, 1098, 1190, 1345, 2235, 2763
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 4 cut(s) 588, 1021, 1223, 2904
SmiMI CAYNNNNRTG 4 cut(s) 1092, 1409, 2868, 2874
SmlI CTYRAG 1 cut(s) 2946
SmoI CTYRAG 1 cut(s) 2946
SnaBI TACGTA 1 cut(s) 1547
SpeI ACTAGT 1 cut(s) 2644
Sse8387I CCTGCAGG 1 cut(s) 2239
SseBI AGGCCT 1 cut(s) 2229
SspMI CTAG 6 cut(s) 708, 1517, 2216, 2645, 2814, 2940
SstI GAGCTC 1 cut(s) 306
StuI AGGCCT 1 cut(s) 2229
StyD4I CCNGG 7 cut(s) 186, 240, 413, 680, 737, 1082, 2519
StyI CCWWGG 5 cut(s) 1806, 1935, 2392, 2634, 2788
TaaI ACNGT 4 cut(s) 79, 1346, 2244, 2426
TaiI ACGT 8 cut(s) 38, 111, 327, 375, 1549, 2700, 2916, 3021
TaqII GACCGA 1 cut(s) 605
TatI WGTACW 4 cut(s) 1171, 1317, 1413, 2426
TauI GCSGC 5 cut(s) 26, 216, 380, 383, 2684
Tru1I TTAA 5 cut(s) 1140, 1758, 1766, 1791, 2840
Tru9I TTAA 5 cut(s) 1140, 1758, 1766, 1791, 2840
TscAI CASTG 9 cut(s) 45, 869, 1199, 1219, 1531, 1558, 2304, 2722, 2830
TseFI GTSAC 7 cut(s) 36, 52, 1153, 1210, 2039, 2086, 2299
TseI GCWGC 4 cut(s) 471, 1097, 1100, 2614
Tsp45I GTSAC 7 cut(s) 36, 52, 1153, 1210, 2039, 2086, 2299
TspDTI ATGAA 4 cut(s) 1652, 2010, 2147, 2889
TspGWI ACGGA 5 cut(s) 71, 247, 1215, 2307, 2790
TspRI CASTG 9 cut(s) 45, 869, 1199, 1219, 1531, 1558, 2304, 2722, 2830
Tth111I GACNNNGTC 2 cut(s) 257, 269
Van91I CCANNNNNTGG 1 cut(s) 1471
VpaK11BI GGWCC 4 cut(s) 588, 1021, 1223, 2904
XapI RAATTY 7 cut(s) 566, 1002, 1786, 1976, 2017, 2481, 2896
XceI RCATGY 7 cut(s) 851, 1097, 1172, 1501, 1511, 2515, 2867
XmiI GTMKAC 2 cut(s) 1735, 3040
XspI CTAG 6 cut(s) 708, 1517, 2216, 2645, 2814, 2940
ZraI GACGTC 3 cut(s) 36, 109, 325
ZrmI AGTACT 1 cut(s) 2428
Zsp2I ATGCAT 4 cut(s) 883, 1612, 1840, 2729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.