Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0013266

Physical Location & Seq

Genomic Coordinates Forward (+)

36646786 .. 36648132

Gene Structure 1347 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1275000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1347 bp

ATGGACATCGCCATGAGACCAAAACAGAGCAGATTATCCAGAACTTTCAGCAAGGTCATCAACCTCCGCACCGCCACGAGCAGAATCGCTTCCAGCAATGGCATTTGCCTCCTTGCGTTGAACGGAAAGTTCAAGGACAATCTCGACCGGCGGAAGGATGACAAGAAGGAAGAAGAGTTCAGAGTCAGGAATAGAGCGGTTATGGAAGCGCTTCTGGCCAAGCTCTTCGCCGGAATCACTTCCATAAAAGCAGCTTACGCGGAGCTCCAAATGGCGCAGGATCCGTACAACAACGAAGCGATTCAGACGGCCGACCAGTCCGTGGTGAACGAGCTGAAATCGATATCGGAGTTGAAACGCAGCTTTTTGAAAAAGGAGCTCGATCTCTCGCCGCAGGTCACTCTGATGCTCGCGGAGATTCAGGAGCAGCAGGCGATGATGAAAACGTACGAGATTACAATCAAGAAACTGGAGTCCGAATCCGAAAAGAAGGATTCCGACATTTCGTCCCTCCAGAAAAAGCTTCAAGATTTGGTCTTGTCCAACAAGTGCTCGGAGAAAAGGCTAAACGACAGCGGCTCTTCGTCTCTCTCCATTTTCGACAACATTCGGCATTCGGAGCTGAATCCGACGCATTTCGTTCAATTCCAGCAGCAGGCTTTGAAATCCATCAAAAGCTTTGTGAGATTGATGATCCGGGAAATGGAATCCGCCAACTGGGATCTCTCCATAGCGGTCGGATTCATTGAACCCGGTTCTGTTTTCGAAAAACAAAGCCATCGATGCTTCGCATTCGAATCCTTCGTTTCCAAAACATTGCTCGAAGGATTCAACAATCCAAACTTCGGCCTACCCAGTGATTCTTTATCCCCAAGCAACAAAACCCACCTTCTGTTTTTCCAGAAATTCAAGAAACTCGTATCCGAAAACCCAAAAAATTTCCTCACCCAGAACCCGAGTTCTTCGTTTTGCAAGTTCACCAAGGCAAAGTACCTCCAGCTCGTTCATGCGAAGATGGAGTGCTCGCTTTTTGGAAATTTGAACATGAGGAAGGTCGTGAGCTCCGGCGGAGTTCCGGACTCTGCTTTTTTCGCGGCGTTTGCAGATGCTGCTATGCGGGTTTGGCTTCTGCATTGCTTGGCGTTCTCGTTTGAGCAGCAAGTTTCGATTTTTCAGGTGAAGAAAGGGTCGAGATTTTCGGAGGTGTTCATGGAGTCAGTGACTGCGGACGACGTCGACATGGAGCCGTTGGTGAGTTTCACGGTTGTTCCCGGGTTTAAGATTGGGAAGACGGTGGTGCAGAGCCTGGTTTACTTGTCTCCGGTCCCTTCTCCGGCGGGAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF641

Protein Analysis

449

Amino Acids

50.42

Weight (kDa)

9.14

Isoelectric Point (pI)

47.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF641	PF04859	64 - 188	1.2e-40	Plant protein of unknown function (DUF641)
GIL1_IRKI_C	PF24994	390 - 438	5.8e-20	GIL1/IRKI C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013266)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29300 AT2G32130
fragaria_vesca	FvH4_3g07250 FvH4_3g07250
malus_domestica	MD00G1008600.v1.1 MD10G1275000.v1.1
prunus_persica	Prupe.4G067400_v2.0.a1
pyrus_communis	pycom05g27380 pycom10g22960
rosa_chinensis	RchiOBHm_Chr5g0011811
rosa_laevigata	RLG00000031863
rosa_multiflora	Rmu_ssc0000357.1_g000004
rosa_rugosa	Rorug04G0459400
rosa_samantha	Rh5AG090900 Rh5BG086500 Rh5CG100300 Rh5DG086500
rosa_wichuraiana	Rw5G007930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1236
Acc36I	ACCTGC	1 cut(s)	385
AccB7I	CCANNNNNTGG	1 cut(s)	322
AccBSI	CCGCTC	1 cut(s)	197
AccI	GTMKAC	1 cut(s)	1236
AccII	CGCG	3 cut(s)	260, 413, 1094
AccIII	TCCGGA	1 cut(s)	1075
AclWI	GGATC	4 cut(s)	275, 288, 688, 729
AcoI	YGGCCR	2 cut(s)	216, 309
AcsI	RAATTY	3 cut(s)	905, 937, 1036
AcyI	GRCGYC	1 cut(s)	1233
AfaI	GTAC	3 cut(s)	287, 449, 992
AfeI	AGCGCT	1 cut(s)	210
AfiI	CCNNNNNNNGG	7 cut(s)	154, 322, 655, 703, 717, 845, 1333
AhdI	GACNNNNNGTC	1 cut(s)	505
AjnI	CCWGG	1 cut(s)	1305
AloI	GAACNNNNNNTCC	2 cut(s)	161, 193
Alw21I	GWGCWC	5 cut(s)	267, 381, 554, 1025, 1064
Alw26I	GTCTC	3 cut(s)	10, 591, 1323
AlwI	GGATC	4 cut(s)	275, 288, 688, 729
AlwNI	CAGNNNCTG	3 cut(s)	1109, 1223, 1306
Ama87I	CYCGRG	2 cut(s)	955, 1271
Aor13HI	TCCGGA	1 cut(s)	1075
Aor51HI	AGCGCT	1 cut(s)	210
AoxI	GGCC	3 cut(s)	216, 309, 847
ApeKI	GCWGC	6 cut(s)	251, 360, 427, 652, 1109, 1156
ApoI	RAATTY	3 cut(s)	905, 937, 1036
Asp700I	GAANNNNTTC	4 cut(s)	88, 210, 238, 300
AspLEI	GCGC	2 cut(s)	211, 277
AspS9I	GGNCC	1 cut(s)	1324
AsuC2I	CCSGG	4 cut(s)	698, 753, 1272, 1273
AsuHPI	GGTGA	5 cut(s)	337, 937, 970, 1189, 1264
AsuII	TTCGAA	2 cut(s)	765, 795
AvaI	CYCGRG	2 cut(s)	955, 1271
AvaII	GGWCC	1 cut(s)	1324
BalI	TGGCCA	1 cut(s)	218
BamHI	GGATCC	1 cut(s)	280
BanII	GRGCYC	3 cut(s)	267, 381, 1064
BauI	CACGAG	1 cut(s)	76
BbsI	GAAGAC	1 cut(s)	1295
Bbv12I	GWGCWC	5 cut(s)	267, 381, 554, 1025, 1064
BbvI	GCAGC	6 cut(s)	263, 372, 439, 664, 1096, 1168
BccI	CCATC	3 cut(s)	677, 786, 1009
BceAI	ACGGC	2 cut(s)	324, 1231
BciT130I	CCWGG	1 cut(s)	1307
BciVI	GTATCC	1 cut(s)	931
BcnI	CCSGG	4 cut(s)	698, 753, 1272, 1273
BcoDI	GTCTC	3 cut(s)	10, 591, 1323
BfoI	RGCGCY	1 cut(s)	212
BfuAI	ACCTGC	1 cut(s)	385
BfuI	GTATCC	1 cut(s)	931
BisI	GCNGC	9 cut(s)	252, 361, 392, 428, 577, 653, 1095, 1110, 1157
BlsI	GCNGC	9 cut(s)	253, 362, 393, 429, 578, 654, 1096, 1111, 1158
Bme1390I	CCNGG	5 cut(s)	698, 753, 1272, 1273, 1307
Bme18I	GGWCC	1 cut(s)	1324
BmeRI	GACNNNNNGTC	1 cut(s)	505
BmeT110I	CYCGRG	2 cut(s)	955, 1271
BmgT120I	GGNCC	1 cut(s)	1324
BmiI	GGNNCC	3 cut(s)	282, 1245, 1326
BmrFI	CCNGG	5 cut(s)	698, 753, 1272, 1273, 1307
BmrI	ACTGGG	2 cut(s)	727, 849
BmsI	GCATC	3 cut(s)	396, 773, 1096
BmuI	ACTGGG	2 cut(s)	727, 849
BpiI	GAAGAC	1 cut(s)	1295
BpmI	CTGGAG	3 cut(s)	491, 497, 980
Bpu14I	TTCGAA	2 cut(s)	765, 795
BpuMI	CCSGG	4 cut(s)	698, 753, 1272, 1273
Bsa29I	ATCGAT	2 cut(s)	341, 781
BsaBI	GATNNNNATC	1 cut(s)	458
BsaHI	GRCGYC	1 cut(s)	1233
BsaI	GGTCTC	1 cut(s)	10
BsaJI	CCNNGG	3 cut(s)	321, 981, 1271
BsaWI	WCCGGW	2 cut(s)	1075, 1321
Bsc4I	CCNNNNNNNGG	7 cut(s)	154, 322, 655, 703, 717, 845, 1333
Bse118I	RCCGGY	1 cut(s)	147
Bse1I	ACTGG	4 cut(s)	316, 474, 722, 855
Bse3DI	GCAATG	3 cut(s)	103, 815, 1132
Bse8I	GATNNNNATC	1 cut(s)	458
BseAI	TCCGGA	1 cut(s)	1075
BseBI	CCWGG	1 cut(s)	1307
BseCI	ATCGAT	2 cut(s)	341, 781
BseDI	CCNNGG	3 cut(s)	321, 981, 1271
BseGI	GGATG	1 cut(s)	163
BseJI	GATNNNNATC	1 cut(s)	458
BseLI	CCNNNNNNNGG	7 cut(s)	154, 322, 655, 703, 717, 845, 1333
BseMI	GCAATG	3 cut(s)	103, 815, 1132
BseNI	ACTGG	4 cut(s)	316, 474, 722, 855
BseX3I	CGGCCG	1 cut(s)	309
BseXI	GCAGC	6 cut(s)	263, 372, 439, 664, 1096, 1168
BsgI	GTGCAG	1 cut(s)	1319
Bsh1236I	CGCG	3 cut(s)	260, 413, 1094
Bsh1285I	CGRYCG	3 cut(s)	148, 312, 738
BshFI	GGCC	3 cut(s)	218, 311, 849
BshVI	ATCGAT	2 cut(s)	341, 781
BsiEI	CGRYCG	3 cut(s)	148, 312, 738
BsiHKAI	GWGCWC	5 cut(s)	267, 381, 554, 1025, 1064
BsiHKCI	CYCGRG	2 cut(s)	955, 1271
BsiSI	CCGG	9 cut(s)	148, 231, 697, 753, 1065, 1076, 1272, 1322, 1334
BsiWI	CGTACG	1 cut(s)	447
BslFI	GGGAC	2 cut(s)	493, 1310
BslI	CCNNNNNNNGG	7 cut(s)	154, 322, 655, 703, 717, 845, 1333
BsmAI	GTCTC	3 cut(s)	10, 591, 1323
BsmBI	CGTCTC	1 cut(s)	591
BsmFI	GGGAC	2 cut(s)	493, 1310
BsmI	GAATGC	2 cut(s)	613, 791
BsnI	GGCC	3 cut(s)	218, 311, 849
Bso31I	GGTCTC	1 cut(s)	10
BsoBI	CYCGRG	2 cut(s)	955, 1271
Bsp119I	TTCGAA	2 cut(s)	765, 795
Bsp1286I	GDGCHC	5 cut(s)	267, 381, 554, 1025, 1064
Bsp13I	TCCGGA	1 cut(s)	1075
Bsp143I	GATC	4 cut(s)	280, 382, 693, 721
BspANI	GGCC	3 cut(s)	218, 311, 849
BspDI	ATCGAT	2 cut(s)	341, 781
BspEI	TCCGGA	1 cut(s)	1075
BspFNI	CGCG	3 cut(s)	260, 413, 1094
BspLI	GGNNCC	3 cut(s)	282, 1245, 1326
BspMI	ACCTGC	1 cut(s)	385
BspPI	GGATC	4 cut(s)	275, 288, 688, 729
BspQI	GCTCTTC	2 cut(s)	230, 586
BspT104I	TTCGAA	2 cut(s)	765, 795
BspTNI	GGTCTC	1 cut(s)	10
BsrBI	CCGCTC	1 cut(s)	197
BsrDI	GCAATG	3 cut(s)	103, 815, 1132
BsrFI	RCCGGY	1 cut(s)	147
BsrI	ACTGG	4 cut(s)	316, 474, 722, 855
BssAI	RCCGGY	1 cut(s)	147
BssECI	CCNNGG	3 cut(s)	321, 981, 1271
BssMI	GATC	4 cut(s)	280, 382, 693, 721
BssNI	GRCGYC	1 cut(s)	1233
BssSI	CACGAG	1 cut(s)	76
BssT1I	CCWWGG	1 cut(s)	981
Bst2BI	CACGAG	1 cut(s)	76
Bst2UI	CCWGG	1 cut(s)	1307
Bst4CI	ACNGT	2 cut(s)	1264, 1294
Bst6I	CTCTTC	3 cut(s)	168, 230, 586
BstACI	GRCGYC	1 cut(s)	1233
BstAPI	GCANNNNNTGC	1 cut(s)	1109
BstBI	TTCGAA	2 cut(s)	765, 795
BstC8I	GCNNGC	4 cut(s)	411, 432, 657, 1025
BstDSI	CCRYGG	1 cut(s)	321
BstF5I	GGATG	1 cut(s)	163
BstFNI	CGCG	3 cut(s)	260, 413, 1094
BstH2I	RGCGCY	1 cut(s)	212
BstHHI	GCGC	2 cut(s)	211, 277
BstKTI	GATC	4 cut(s)	283, 385, 696, 724
BstMAI	GTCTC	3 cut(s)	10, 591, 1323
BstMBI	GATC	4 cut(s)	280, 382, 693, 721
BstMCI	CGRYCG	3 cut(s)	148, 312, 738
BstMWI	GCNNNNNNNGC	8 cut(s)	215, 257, 619, 783, 1091, 1100, 1109, 1123
BstNI	CCWGG	1 cut(s)	1307
BstSCI	CCNGG	5 cut(s)	696, 751, 1270, 1271, 1305
BstUI	CGCG	3 cut(s)	260, 413, 1094
BstV1I	GCAGC	6 cut(s)	263, 372, 439, 664, 1096, 1168
BstV2I	GAAGAC	1 cut(s)	1295
BstX2I	RGATCY	2 cut(s)	280, 721
BstYI	RGATCY	2 cut(s)	280, 721
BstZI	CGGCCG	1 cut(s)	309
Bsu15I	ATCGAT	2 cut(s)	341, 781
BsuI	GTATCC	1 cut(s)	931
BsuRI	GGCC	3 cut(s)	218, 311, 849
BsuTUI	ATCGAT	2 cut(s)	341, 781
BtgI	CCRYGG	1 cut(s)	321
BtgZI	GCGATG	1 cut(s)	449
BtsCI	GGATG	1 cut(s)	163
BtsIMutI	CAGTG	2 cut(s)	862, 1224
BveI	ACCTGC	1 cut(s)	385
Cac8I	GCNNGC	4 cut(s)	411, 432, 657, 1025
CaiI	CAGNNNCTG	3 cut(s)	1109, 1223, 1306
CfoI	GCGC	2 cut(s)	211, 277
Cfr10I	RCCGGY	1 cut(s)	147
Cfr13I	GGNCC	1 cut(s)	1324
Cfr9I	CCCGGG	1 cut(s)	1271
ClaI	ATCGAT	2 cut(s)	341, 781
CseI	GACGC	1 cut(s)	640
Csp6I	GTAC	3 cut(s)	286, 448, 991
CviAII	CATG	5 cut(s)	13, 1007, 1045, 1210, 1240
CviQI	GTAC	3 cut(s)	286, 448, 991
DpnI	GATC	4 cut(s)	282, 384, 695, 723
DpnII	GATC	4 cut(s)	280, 382, 693, 721
DriI	GACNNNNNGTC	1 cut(s)	505
EaeI	YGGCCR	2 cut(s)	216, 309
EagI	CGGCCG	1 cut(s)	309
Eam1104I	CTCTTC	3 cut(s)	168, 230, 586
Eam1105I	GACNNNNNGTC	1 cut(s)	505
EarI	CTCTTC	3 cut(s)	168, 230, 586
EciI	GGCGGA	3 cut(s)	166, 700, 1083
Ecl136II	GAGCTC	3 cut(s)	265, 379, 1062
EclXI	CGGCCG	1 cut(s)	309
Eco130I	CCWWGG	1 cut(s)	981
Eco24I	GRGCYC	3 cut(s)	267, 381, 1064
Eco31I	GGTCTC	1 cut(s)	10
Eco32I	GATATC	1 cut(s)	345
Eco47I	GGWCC	1 cut(s)	1324
Eco47III	AGCGCT	1 cut(s)	210
Eco52I	CGGCCG	1 cut(s)	309
Eco53kI	GAGCTC	3 cut(s)	265, 379, 1062
Eco88I	CYCGRG	2 cut(s)	955, 1271
EcoICRI	GAGCTC	3 cut(s)	265, 379, 1062
EcoRII	CCWGG	1 cut(s)	1305
EcoRV	GATATC	1 cut(s)	345
EcoT14I	CCWWGG	1 cut(s)	981
EcoT38I	GRGCYC	3 cut(s)	267, 381, 1064
ErhI	CCWWGG	1 cut(s)	981
Esp3I	CGTCTC	1 cut(s)	591
FaeI	CATG	5 cut(s)	16, 1010, 1048, 1213, 1243
FaiI	YATR	9 cut(s)	14, 203, 245, 731, 1008, 1046, 1115, 1211, 1241
FaqI	GGGAC	2 cut(s)	493, 1310
FatI	CATG	5 cut(s)	12, 1006, 1044, 1209, 1239
FauI	CCCGC	2 cut(s)	1110, 1330
FblI	GTMKAC	1 cut(s)	1236
Fnu4HI	GCNGC	9 cut(s)	252, 361, 392, 428, 577, 653, 1095, 1110, 1157
FokI	GGATG	1 cut(s)	170
FriOI	GRGCYC	3 cut(s)	267, 381, 1064
Fsp4HI	GCNGC	9 cut(s)	252, 361, 392, 428, 577, 653, 1095, 1110, 1157
GlaI	GCGC	2 cut(s)	210, 276
GluI	GCNGC	9 cut(s)	252, 361, 392, 428, 577, 653, 1095, 1110, 1157
GsuI	CTGGAG	3 cut(s)	491, 497, 980
HaeII	RGCGCY	1 cut(s)	212
HaeIII	GGCC	3 cut(s)	218, 311, 849
HapII	CCGG	9 cut(s)	148, 231, 697, 753, 1065, 1076, 1272, 1322, 1334
HgaI	GACGC	1 cut(s)	640
HhaI	GCGC	2 cut(s)	211, 277
Hin1I	GRCGYC	1 cut(s)	1233
Hin1II	CATG	5 cut(s)	16, 1010, 1048, 1213, 1243
Hin6I	GCGC	2 cut(s)	209, 275
HinP1I	GCGC	2 cut(s)	209, 275
HincII	GTYRAC	1 cut(s)	1237
HindII	GTYRAC	1 cut(s)	1237
HindIII	AAGCTT	2 cut(s)	521, 676
HpaII	CCGG	9 cut(s)	148, 231, 697, 753, 1065, 1076, 1272, 1322, 1334
HphI	GGTGA	5 cut(s)	337, 937, 970, 1189, 1264
Hpy166II	GTNNAC	4 cut(s)	328, 978, 1237, 1312
Hpy8I	GTNNAC	4 cut(s)	328, 978, 1237, 1312
Hpy99I	CGWCG	3 cut(s)	634, 1235, 1238
HpyAV	CCTTC	8 cut(s)	148, 160, 484, 811, 818, 899, 1045, 1338
HpyCH4III	ACNGT	2 cut(s)	1264, 1294
HpyCH4IV	ACGT	2 cut(s)	446, 1233
HpyCH4V	TGCA	4 cut(s)	972, 1103, 1132, 1300
HpyF10VI	GCNNNNNNNGC	8 cut(s)	215, 257, 619, 783, 1091, 1100, 1109, 1123
HpySE526I	ACGT	2 cut(s)	446, 1233
Hsp92I	GRCGYC	1 cut(s)	1233
Hsp92II	CATG	5 cut(s)	16, 1010, 1048, 1213, 1243
HspAI	GCGC	2 cut(s)	209, 275
Kpn2I	TCCGGA	1 cut(s)	1075
Kzo9I	GATC	4 cut(s)	280, 382, 693, 721
LguI	GCTCTTC	2 cut(s)	230, 586
LmnI	GCTCC	7 cut(s)	262, 270, 376, 424, 619, 1067, 1243
Lsp1109I	GCAGC	6 cut(s)	263, 372, 439, 664, 1096, 1168
LweI	GCATC	3 cut(s)	396, 773, 1096
MaeII	ACGT	2 cut(s)	446, 1233
MaeIII	GTNAC	2 cut(s)	397, 1219
MalI	GATC	4 cut(s)	282, 384, 695, 723
MbiI	CCGCTC	1 cut(s)	197
MboI	GATC	4 cut(s)	280, 382, 693, 721
MboII	GAAGA	8 cut(s)	182, 185, 217, 573, 954, 1024, 1192, 1300
MflI	RGATCY	2 cut(s)	280, 721
MhlI	GDGCHC	5 cut(s)	267, 381, 554, 1025, 1064
MlsI	TGGCCA	1 cut(s)	218
MluCI	AATT	4 cut(s)	644, 905, 937, 1036
MluNI	TGGCCA	1 cut(s)	218
MlyI	GAGTC	4 cut(s)	192, 482, 1073, 1223
MmeI	TCCRAC	4 cut(s)	522, 567, 653, 718
MnlI	CCTC	7 cut(s)	74, 119, 521, 953, 1004, 1041, 1195
Mox20I	TGGCCA	1 cut(s)	218
MroI	TCCGGA	1 cut(s)	1075
MroXI	GAANNNNTTC	4 cut(s)	88, 210, 238, 300
MscI	TGGCCA	1 cut(s)	218
MseI	TTAA	2 cut(s)	1278, 1345
MslI	CAYNNNNRTG	2 cut(s)	11, 404
Msp20I	TGGCCA	1 cut(s)	218
MspA1I	CMGCKG	1 cut(s)	576
MspI	CCGG	9 cut(s)	148, 231, 697, 753, 1065, 1076, 1272, 1322, 1334
MspR9I	CCNGG	5 cut(s)	698, 753, 1272, 1273, 1307
Mva1269I	GAATGC	2 cut(s)	613, 791
MvaI	CCWGG	1 cut(s)	1307
MvnI	CGCG	3 cut(s)	260, 413, 1094
MwoI	GCNNNNNNNGC	8 cut(s)	215, 257, 619, 783, 1091, 1100, 1109, 1123
NciI	CCSGG	4 cut(s)	698, 753, 1272, 1273
NdeII	GATC	4 cut(s)	280, 382, 693, 721
NlaIII	CATG	5 cut(s)	16, 1010, 1048, 1213, 1243
NlaIV	GGNNCC	3 cut(s)	282, 1245, 1326
NmuCI	GTSAC	2 cut(s)	397, 1219
NspV	TTCGAA	2 cut(s)	765, 795
PciSI	GCTCTTC	2 cut(s)	230, 586
PcsI	WCGNNNNNNNCGW	1 cut(s)	801
PctI	GAATGC	2 cut(s)	613, 791
PdmI	GAANNNNTTC	4 cut(s)	88, 210, 238, 300
Pfl23II	CGTACG	1 cut(s)	447
PflFI	GACNNNGTC	1 cut(s)	1232
PflMI	CCANNNNNTGG	1 cut(s)	322
PfoI	TCCNGGA	1 cut(s)	696
PkrI	GCNGC	9 cut(s)	253, 362, 393, 429, 578, 654, 1096, 1111, 1158
PleI	GAGTC	4 cut(s)	191, 481, 1073, 1222
PpsI	GAGTC	4 cut(s)	191, 481, 1073, 1222
Psp124BI	GAGCTC	3 cut(s)	267, 381, 1064
Psp6I	CCWGG	1 cut(s)	1305
PspGI	CCWGG	1 cut(s)	1305
PspLI	CGTACG	1 cut(s)	447
PspN4I	GGNNCC	3 cut(s)	282, 1245, 1326
PspPI	GGNCC	1 cut(s)	1324
PstNI	CAGNNNCTG	3 cut(s)	1109, 1223, 1306
PsuI	RGATCY	2 cut(s)	280, 721
PsyI	GACNNNGTC	1 cut(s)	1232
RsaI	GTAC	3 cut(s)	287, 449, 992
RsaNI	GTAC	3 cut(s)	286, 448, 991
RseI	CAYNNNNRTG	2 cut(s)	11, 404
SacI	GAGCTC	3 cut(s)	267, 381, 1064
SalI	GTCGAC	1 cut(s)	1235
SapI	GCTCTTC	2 cut(s)	230, 586
SaqAI	TTAA	2 cut(s)	1278, 1345
SatI	GCNGC	9 cut(s)	252, 361, 392, 428, 577, 653, 1095, 1110, 1157
Sau3AI	GATC	4 cut(s)	280, 382, 693, 721
Sau96I	GGNCC	1 cut(s)	1324
SchI	GAGTC	4 cut(s)	192, 482, 1073, 1223
ScrFI	CCNGG	5 cut(s)	698, 753, 1272, 1273, 1307
SduI	GDGCHC	5 cut(s)	267, 381, 554, 1025, 1064
SfaNI	GCATC	3 cut(s)	396, 773, 1096
SfuI	TTCGAA	2 cut(s)	765, 795
SinI	GGWCC	1 cut(s)	1324
SmaI	CCCGGG	1 cut(s)	1273
SmiMI	CAYNNNNRTG	2 cut(s)	11, 404
Sse9I	AATT	4 cut(s)	644, 905, 937, 1036
SstI	GAGCTC	3 cut(s)	267, 381, 1064
StyD4I	CCNGG	5 cut(s)	696, 751, 1270, 1271, 1305
StyI	CCWWGG	1 cut(s)	981
TaaI	ACNGT	2 cut(s)	1264, 1294
TaiI	ACGT	2 cut(s)	449, 1236
TasI	AATT	4 cut(s)	644, 905, 937, 1036
TauI	GCSGC	3 cut(s)	394, 579, 1097
Tru1I	TTAA	2 cut(s)	1278, 1345
Tru9I	TTAA	2 cut(s)	1278, 1345
TscAI	CASTG	2 cut(s)	862, 1224
TseFI	GTSAC	2 cut(s)	397, 1219
TseI	GCWGC	6 cut(s)	251, 360, 427, 652, 1109, 1156
Tsp45I	GTSAC	2 cut(s)	397, 1219
TspDTI	ATGAA	4 cut(s)	455, 733, 995, 1198
TspGWI	ACGGA	3 cut(s)	138, 273, 310
TspMI	CCCGGG	1 cut(s)	1271
TspRI	CASTG	2 cut(s)	862, 1224
Tth111I	GACNNNGTC	1 cut(s)	1232
Van91I	CCANNNNNTGG	1 cut(s)	322
VpaK11BI	GGWCC	1 cut(s)	1324
XapI	RAATTY	3 cut(s)	905, 937, 1036
XmaI	CCCGGG	1 cut(s)	1271
XmiI	GTMKAC	1 cut(s)	1236
XmnI	GAANNNNTTC	4 cut(s)	88, 210, 238, 300
ZraI	GACGTC	1 cut(s)	1234

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.