RLG00000031863

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013266
Physical Location & Seq
Reverse (-)
8862954 .. 8864303
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031863

Sequence Viewer

Length: 1350 bp
ATGGATACTATCAGACCCAAATCGGCTCCGATCAAGCGGAGCAAGCTGGTCAGGACTTTCCACAAGGTCATCAGCCTCCGAGCCGCCACCAAAATCGCTTCCAACAATGGCATTTGCAAGCTCAAACCGAATACCAAAGCCACCAAAGAGGAGTACGATTTCGAAAAGATGGAGGATCTCAAAGCCAAGAACAGAGCAGTCATGGAGGCTTTACTGGCCAAGCTCTTCGCCGGAATCACTTCCATTAAAGCAGCCTATGCCGAGCTCCAAATGGCCCAAGACCCTTACAACAACGAAGCCATTCAGGTGGCTGACCAGGCCGTAGTGGACGAGCTGAAAGCCATTTCAGAACTCAAACGCAGCTTCTTGAAGAAAGAGCTCGATCTCTCGCCGCAAGTCACTCTAATGCTGGCGGAGATTCAAGAACAACAGGGACTAATGAAGACATACGAGATCACTATCAAGAAACTAGAGTCCGAAGCTCACCGCAAAGGTTTAGGCATTTCCACACTCAAATCCGAACTCGAAGAATTAGTCTCAACCAACAACTCCTTCGAATACAAGCTCAAATCCAGCGGACCTCTTTCAATCTTCGACAATCTTCTCCTCTCGAAGCTAACCGCAACCCATTTCGCTCAGTTTCAGCAGCAGACGGTAAGATCCATACGAAGCTTTGTCAGATTGATGATCAAAGATATGGAATCCGCAAGCTGGGACCTCGACACAGCCATCAAGTTCATCGAGCCAGAAGCAGAGTTCTCGAAACAGAGCCATCGCTGCTTCGCATTCGAGTCCTTCGTCAGCAAAACCATGCTCGAAGGCTTCAACAACCCCAACTTCGGACTCCCCAGCGACTCATTCCCTCCCAATCACAAAACCCACCACCACTTTTTCGACCAGTTCAACAAGCTCAACCCCGCAAACCCAAACTCGTTCCTCGCCCAAAACCACAGCGCGTCGTTTTGCAAGTTCACCAGAGCCAAGTACCTCCAACTGGTACACGCCAAAATGGAGTGCTCCCTCTTCGGAAACTTAAACCAGAGAAAGCTCATAAACTCCGCCGAGGTTCCCGACACTCCGTTTTTCGAAGCCTTTGCAGAAATGTCGATGCGGGTTTGGCTTCTACACTGCTTGGCATTCTCGTTCGGTCAACAAGTCTCGATCTTTCAGGTCAACAAAAACCGCAGATTTTCAGAGGTGTTCATGGAGTCCGTCACCGTCAACAACGACTCCGACACCGAGTTTTCCGTCAGCTTCACCGTCATGCCGGGGTTCAGAATCGGGAAGACGGTGTTTCAGAGCCTGGTTTACTTGTCTCCGGTGACTAATCTCCGGCGAATTTACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

450

Amino Acids

51.19

Weight (kDa)

9.03

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 64 - 187 7.5e-40 Plant protein of unknown function (DUF641)
GIL1_IRKI_C PF24994 388 - 437 4.9e-17 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29300 AT2G32130
fragaria_vesca FvH4_3g07250 FvH4_3g07250
malus_domestica MD00G1008600.v1.1 MD10G1275000.v1.1
prunus_persica Prupe.4G067400_v2.0.a1
pyrus_communis pycom05g27380 pycom10g22960
rosa_chinensis RchiOBHm_Chr5g0011811
rosa_laevigata RLG00000031863
rosa_multiflora Rmu_ssc0000357.1_g000004
rosa_rugosa Rorug04G0459400
rosa_samantha Rh5AG090900 Rh5BG086500 Rh5CG100300 Rh5DG086500
rosa_wichuraiana Rw5G007930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 956
AclWI GGATC 2 cut(s) 183, 654
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 1 cut(s) 1338
AfaI GTAC 3 cut(s) 155, 986, 999
AfiI CCNNNNNNNGG 3 cut(s) 711, 839, 994
AgsI TTSAA 5 cut(s) 370, 422, 588, 826, 904
AjnI CCWGG 2 cut(s) 315, 1302
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
Alw21I GWGCWC 3 cut(s) 267, 381, 1019
Alw26I GTCTC 3 cut(s) 541, 1162, 1320
AlwI GGATC 2 cut(s) 183, 654
AlwNI CAGNNNCTG 1 cut(s) 1303
AoxI GGCC 3 cut(s) 216, 273, 318
ApeKI GCWGC 4 cut(s) 251, 360, 646, 777
ApoI RAATTY 1 cut(s) 1338
ArsI GACNNNNNNTTYG 2 cut(s) 519, 551
Asp700I GAANNNNTTC 2 cut(s) 238, 300
AspLEI GCGC 1 cut(s) 956
AspS9I GGNCC 3 cut(s) 274, 578, 715
AsuC2I CCSGG 1 cut(s) 1269
AsuHPI GGTGA 5 cut(s) 476, 964, 1207, 1249, 1333
AsuII TTCGAA 3 cut(s) 162, 555, 1086
AvaII GGWCC 2 cut(s) 578, 715
BalI TGGCCA 1 cut(s) 218
BanII GRGCYC 2 cut(s) 267, 381
BbsI GAAGAC 2 cut(s) 449, 1292
Bbv12I GWGCWC 3 cut(s) 267, 381, 1019
BbvI GCAGC 4 cut(s) 263, 372, 658, 764
BccI CCATC 3 cut(s) 163, 737, 780
BceAI ACGGC 1 cut(s) 305
BcgI CGANNNNNNTGC 6 cut(s) 741, 775, 1076, 1086, 1110, 1120
BciT130I CCWGG 2 cut(s) 317, 1304
BclI TGATCA 1 cut(s) 687
BcnI CCSGG 1 cut(s) 1269
BcoDI GTCTC 3 cut(s) 541, 1162, 1320
BfaI CTAG 1 cut(s) 470
BisI GCNGC 6 cut(s) 84, 252, 361, 392, 647, 778
BlsI GCNGC 6 cut(s) 85, 253, 362, 393, 648, 779
Bme1390I CCNGG 3 cut(s) 317, 1269, 1304
Bme18I GGWCC 2 cut(s) 578, 715
BmgT120I GGNCC 3 cut(s) 274, 578, 715
BmiI GGNNCC 3 cut(s) 27, 716, 1068
BmrFI CCNGG 3 cut(s) 317, 1269, 1304
BmsI GCATC 1 cut(s) 1098
BpiI GAAGAC 2 cut(s) 449, 1292
Bpu14I TTCGAA 3 cut(s) 162, 555, 1086
BpuMI CCSGG 1 cut(s) 1269
BsaBI GATNNNNATC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 1062, 1268
BsaWI WCCGGW 1 cut(s) 1318
BsaXI ACNNNNNCTCC 2 cut(s) 1214, 1244
Bsc4I CCNNNNNNNGG 3 cut(s) 711, 839, 994
Bse1I ACTGG 3 cut(s) 219, 898, 999
Bse8I GATNNNNATC 1 cut(s) 458
BseBI CCWGG 2 cut(s) 317, 1304
BseDI CCNNGG 2 cut(s) 1062, 1268
BseJI GATNNNNATC 1 cut(s) 458
BseLI CCNNNNNNNGG 3 cut(s) 711, 839, 994
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 3 cut(s) 219, 898, 999
BseRI GAGGAG 2 cut(s) 164, 596
BseXI GCAGC 4 cut(s) 263, 372, 658, 764
BseYI CCCAGC 2 cut(s) 711, 848
Bsh1236I CGCG 1 cut(s) 956
BshFI GGCC 3 cut(s) 218, 275, 320
BsiHKAI GWGCWC 3 cut(s) 267, 381, 1019
BsiSI CCGG 4 cut(s) 231, 1268, 1319, 1333
BslFI GGGAC 2 cut(s) 447, 728
BslI CCNNNNNNNGG 3 cut(s) 711, 839, 994
BsmAI GTCTC 3 cut(s) 541, 1162, 1320
BsmFI GGGAC 2 cut(s) 447, 728
BsmI GAATGC 2 cut(s) 785, 1136
BsnI GGCC 3 cut(s) 218, 275, 320
Bsp119I TTCGAA 3 cut(s) 162, 555, 1086
Bsp1286I GDGCHC 3 cut(s) 267, 381, 1019
Bsp143I GATC 7 cut(s) 30, 175, 382, 453, 659, 687, 1161
BspANI GGCC 3 cut(s) 218, 275, 320
BspCNI CTCAG 1 cut(s) 649
BspFNI CGCG 1 cut(s) 956
BspLI GGNNCC 3 cut(s) 27, 716, 1068
BspPI GGATC 2 cut(s) 183, 654
BspQI GCTCTTC 1 cut(s) 230
BspT104I TTCGAA 3 cut(s) 162, 555, 1086
BsrI ACTGG 3 cut(s) 219, 898, 999
BssECI CCNNGG 2 cut(s) 1062, 1268
BssMI GATC 7 cut(s) 30, 175, 382, 453, 659, 687, 1161
Bst2UI CCWGG 2 cut(s) 317, 1304
Bst4CI ACNGT 4 cut(s) 655, 1219, 1261, 1291
Bst6I CTCTTC 2 cut(s) 230, 1028
BstAPI GCANNNNNTGC 1 cut(s) 257
BstBI TTCGAA 3 cut(s) 162, 555, 1086
BstC8I GCNNGC 4 cut(s) 44, 119, 411, 709
BstDEI CTNAG 1 cut(s) 636
BstFNI CGCG 1 cut(s) 956
BstHHI GCGC 1 cut(s) 956
BstKTI GATC 7 cut(s) 33, 178, 385, 456, 662, 690, 1164
BstMAI GTCTC 3 cut(s) 541, 1162, 1320
BstMBI GATC 7 cut(s) 30, 175, 382, 453, 659, 687, 1161
BstMWI GCNNNNNNNGC 6 cut(s) 43, 215, 257, 317, 777, 1117
BstNI CCWGG 2 cut(s) 317, 1304
BstSCI CCNGG 3 cut(s) 315, 1267, 1302
BstUI CGCG 1 cut(s) 956
BstV1I GCAGC 4 cut(s) 263, 372, 658, 764
BstV2I GAAGAC 2 cut(s) 449, 1292
BstX2I RGATCY 2 cut(s) 175, 659
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 2 cut(s) 175, 659
BsuRI GGCC 3 cut(s) 218, 275, 320
BtgZI GCGATG 1 cut(s) 758
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 1 cut(s) 1126
Cac8I GCNNGC 4 cut(s) 44, 119, 411, 709
CaiI CAGNNNCTG 1 cut(s) 1303
CfoI GCGC 1 cut(s) 956
Cfr13I GGNCC 3 cut(s) 274, 578, 715
CseI GACGC 1 cut(s) 945
Csp6I GTAC 3 cut(s) 154, 985, 998
CviAII CATG 4 cut(s) 202, 811, 1204, 1264
CviQI GTAC 3 cut(s) 154, 985, 998
DdeI CTNAG 1 cut(s) 636
DpnI GATC 7 cut(s) 32, 177, 384, 455, 661, 689, 1163
DpnII GATC 7 cut(s) 30, 175, 382, 453, 659, 687, 1161
EaeI YGGCCR 1 cut(s) 216
Eam1104I CTCTTC 2 cut(s) 230, 1028
EarI CTCTTC 2 cut(s) 230, 1028
EciI GGCGGA 2 cut(s) 428, 1048
Ecl136II GAGCTC 2 cut(s) 265, 379
Eco24I GRGCYC 2 cut(s) 267, 381
Eco47I GGWCC 2 cut(s) 578, 715
Eco53kI GAGCTC 2 cut(s) 265, 379
EcoICRI GAGCTC 2 cut(s) 265, 379
EcoO109I RGGNCCY 1 cut(s) 715
EcoRII CCWGG 2 cut(s) 315, 1302
EcoT38I GRGCYC 2 cut(s) 267, 381
FaeI CATG 4 cut(s) 205, 814, 1207, 1267
FaiI YATR 9 cut(s) 203, 258, 448, 665, 698, 812, 1052, 1205, 1265
FaqI GGGAC 2 cut(s) 447, 728
FatI CATG 4 cut(s) 201, 810, 1203, 1263
FauI CCCGC 2 cut(s) 925, 1104
FbaI TGATCA 1 cut(s) 687
Fnu4HI GCNGC 6 cut(s) 84, 252, 361, 392, 647, 778
FriOI GRGCYC 2 cut(s) 267, 381
Fsp4HI GCNGC 6 cut(s) 84, 252, 361, 392, 647, 778
FspBI CTAG 1 cut(s) 470
GlaI GCGC 1 cut(s) 955
GluI GCNGC 6 cut(s) 84, 252, 361, 392, 647, 778
GsaI CCCAGC 2 cut(s) 715, 852
HaeIII GGCC 3 cut(s) 218, 275, 320
HapII CCGG 4 cut(s) 231, 1268, 1319, 1333
HgaI GACGC 1 cut(s) 945
HhaI GCGC 1 cut(s) 956
Hin1II CATG 4 cut(s) 205, 814, 1207, 1267
Hin6I GCGC 1 cut(s) 954
HinP1I GCGC 1 cut(s) 954
HincII GTYRAC 3 cut(s) 1151, 1174, 1222
HindII GTYRAC 3 cut(s) 1151, 1174, 1222
HindIII AAGCTT 1 cut(s) 670
HpaII CCGG 4 cut(s) 231, 1268, 1319, 1333
HphI GGTGA 5 cut(s) 476, 964, 1207, 1249, 1333
Hpy166II GTNNAC 7 cut(s) 328, 972, 1000, 1151, 1174, 1222, 1309
Hpy188III TCNNGA 9 cut(s) 52, 367, 422, 463, 610, 760, 1070, 1159, 1282
Hpy8I GTNNAC 7 cut(s) 328, 972, 1000, 1151, 1174, 1222, 1309
Hpy99I CGWCG 1 cut(s) 961
HpyAV CCTTC 3 cut(s) 562, 805, 812
HpyCH4III ACNGT 4 cut(s) 655, 1219, 1261, 1291
HpyCH4V TGCA 3 cut(s) 117, 966, 1097
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 215, 257, 317, 777, 1117
HpyF3I CTNAG 1 cut(s) 636
Hsp92II CATG 4 cut(s) 205, 814, 1207, 1267
HspAI GCGC 1 cut(s) 954
Ksp22I TGATCA 1 cut(s) 687
Kzo9I GATC 7 cut(s) 30, 175, 382, 453, 659, 687, 1161
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 4 cut(s) 31, 39, 270, 1022
Lsp1109I GCAGC 4 cut(s) 263, 372, 658, 764
LweI GCATC 1 cut(s) 1098
MaeI CTAG 1 cut(s) 470
MaeIII GTNAC 3 cut(s) 397, 1213, 1321
MalI GATC 7 cut(s) 32, 177, 384, 455, 661, 689, 1163
MboI GATC 7 cut(s) 30, 175, 382, 453, 659, 687, 1161
MboII GAAGA 8 cut(s) 217, 382, 454, 539, 583, 593, 1015, 1297
MflI RGATCY 2 cut(s) 175, 659
MhlI GDGCHC 3 cut(s) 267, 381, 1019
MlsI TGGCCA 1 cut(s) 218
MluCI AATT 2 cut(s) 530, 1338
MluNI TGGCCA 1 cut(s) 218
MlyI GAGTC 6 cut(s) 482, 800, 837, 848, 1217, 1223
MmeI TCCRAC 3 cut(s) 126, 1015, 1257
Mox20I TGGCCA 1 cut(s) 218
MroXI GAANNNNTTC 2 cut(s) 238, 300
MscI TGGCCA 1 cut(s) 218
MseI TTAA 2 cut(s) 246, 1034
MslI CAYNNNNRTG 3 cut(s) 305, 404, 1262
Msp20I TGGCCA 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 576
MspI CCGG 4 cut(s) 231, 1268, 1319, 1333
MspR9I CCNGG 3 cut(s) 317, 1269, 1304
Mva1269I GAATGC 2 cut(s) 785, 1136
MvaI CCWGG 2 cut(s) 317, 1304
MvnI CGCG 1 cut(s) 956
MwoI GCNNNNNNNGC 6 cut(s) 43, 215, 257, 317, 777, 1117
NciI CCSGG 1 cut(s) 1269
NdeII GATC 7 cut(s) 30, 175, 382, 453, 659, 687, 1161
NlaIII CATG 4 cut(s) 205, 814, 1207, 1267
NlaIV GGNNCC 3 cut(s) 27, 716, 1068
NmeAIII GCCGAG 2 cut(s) 286, 1087
NmuCI GTSAC 3 cut(s) 397, 1213, 1321
NspV TTCGAA 3 cut(s) 162, 555, 1086
PciSI GCTCTTC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 1 cut(s) 795
PctI GAATGC 2 cut(s) 785, 1136
PdmI GAANNNNTTC 2 cut(s) 238, 300
PfeI GAWTC 4 cut(s) 234, 418, 701, 1278
PkrI GCNGC 6 cut(s) 85, 253, 362, 393, 648, 779
PleI GAGTC 6 cut(s) 481, 799, 837, 848, 1216, 1223
PpsI GAGTC 6 cut(s) 481, 799, 837, 848, 1216, 1223
PpuMI RGGWCCY 1 cut(s) 715
Psp124BI GAGCTC 2 cut(s) 267, 381
Psp5II RGGWCCY 1 cut(s) 715
Psp6I CCWGG 2 cut(s) 315, 1302
PspFI CCCAGC 2 cut(s) 711, 848
PspGI CCWGG 2 cut(s) 315, 1302
PspN4I GGNNCC 3 cut(s) 27, 716, 1068
PspPI GGNCC 3 cut(s) 274, 578, 715
PspPPI RGGWCCY 1 cut(s) 715
PstNI CAGNNNCTG 1 cut(s) 1303
PsuI RGATCY 2 cut(s) 175, 659
RsaI GTAC 3 cut(s) 155, 986, 999
RsaNI GTAC 3 cut(s) 154, 985, 998
RseI CAYNNNNRTG 3 cut(s) 305, 404, 1262
SacI GAGCTC 2 cut(s) 267, 381
SapI GCTCTTC 1 cut(s) 230
SaqAI TTAA 2 cut(s) 246, 1034
SatI GCNGC 6 cut(s) 84, 252, 361, 392, 647, 778
Sau3AI GATC 7 cut(s) 30, 175, 382, 453, 659, 687, 1161
Sau96I GGNCC 3 cut(s) 274, 578, 715
SchI GAGTC 6 cut(s) 482, 800, 837, 848, 1217, 1223
ScrFI CCNGG 3 cut(s) 317, 1269, 1304
SduI GDGCHC 3 cut(s) 267, 381, 1019
SfaNI GCATC 1 cut(s) 1098
SfuI TTCGAA 3 cut(s) 162, 555, 1086
SinI GGWCC 2 cut(s) 578, 715
SmiMI CAYNNNNRTG 3 cut(s) 305, 404, 1262
Sse9I AATT 2 cut(s) 530, 1338
SspMI CTAG 1 cut(s) 470
SstI GAGCTC 2 cut(s) 267, 381
StyD4I CCNGG 3 cut(s) 315, 1267, 1302
TaaI ACNGT 4 cut(s) 655, 1219, 1261, 1291
TaqII GACCGA 1 cut(s) 1136
TasI AATT 2 cut(s) 530, 1338
TauI GCSGC 2 cut(s) 86, 394
TfiI GAWTC 4 cut(s) 234, 418, 701, 1278
Tru1I TTAA 2 cut(s) 246, 1034
Tru9I TTAA 2 cut(s) 246, 1034
TscAI CASTG 1 cut(s) 1133
TseFI GTSAC 3 cut(s) 397, 1213, 1321
TseI GCWGC 4 cut(s) 251, 360, 646, 777
Tsp45I GTSAC 3 cut(s) 397, 1213, 1321
TspDTI ATGAA 3 cut(s) 455, 727, 1192
TspGWI ACGGA 3 cut(s) 1068, 1201, 1237
TspRI CASTG 1 cut(s) 1133
VpaK11BI GGWCC 2 cut(s) 578, 715
XapI RAATTY 1 cut(s) 1338
XmnI GAANNNNTTC 2 cut(s) 238, 300
XspI CTAG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.