MD10G1326900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0017934
Physical Location & Seq
Forward (+)
40686530 .. 40687093
564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1326900.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGCATTTCGCACCAGTCGAACAATACAGAACCGGAGCGCGCGCAACAGCGCGGGCCGTCTCAATCACTCCGTCGATGCGGGACTCTTATCCGCCGTACATCCAAACTTAGTCGATCGCGATGACGAAGATTCTCATGCGACGCTGAGTTCCGACTCGGACAGCTCCTCCGACTCCGATGGCGAGTCGACGCGGTTGTCGGGACTCGAGGCGAGCTTCCGAGTCGTGTCCGAGTCGATTCAGAGAATGGAGCAAGCGGAGCTGGAGATGGCCAAGGCAAGGGAGGCTCTGCGGCTGAAGGCGGAGAAGCAGCGGGTCGAGCTGGAGACCGAGTTGACACAGATGCTGCTGCAGACTCAGTTGCAGATCGCGTCGTTGGTTTCTCAGCGGCAGAGTCGTCCGAGTCGTAAAAGGAAGCGAGTCGAAGAAGACGAGCCGTCGTCGGAAGGAGCTTTGGGCTTGAGCCTCCTTCAATGCAATTTACTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

18.13

Weight (kDa)

5.48

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017934)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22160 AT4G22160
fragaria_vesca FvH4_5g10500
malus_domestica MD10G1326900.v1.1
prunus_persica Prupe.4G015100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002091
rosa_laevigata RLG00000030993
rosa_multiflora Rmu_co8270171.1_g000001
rosa_rugosa Rorug04G0394400
rosa_samantha Rh5BG019200
rosa_wichuraiana Rw5G001550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 188
AccII CGCG 6 cut(s) 41, 43, 53, 120, 193, 371
AciI CCGC 9 cut(s) 53, 80, 93, 193, 257, 292, 302, 313, 388
AcoI YGGCCR 1 cut(s) 270
AcuI CTGAAG 1 cut(s) 317
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 279
AgsI TTSAA 1 cut(s) 473
AhdI GACNNNNNGTC 1 cut(s) 436
AluBI AGCT 5 cut(s) 165, 216, 262, 322, 452
AluI AGCT 5 cut(s) 165, 216, 262, 322, 452
Alw26I GTCTC 2 cut(s) 65, 320
AlwNI CAGNNNCTG 1 cut(s) 346
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 2 cut(s) 55, 270
ApeKI GCWGC 3 cut(s) 310, 346, 349
AspLEI GCGC 4 cut(s) 41, 43, 45, 53
AspS9I GGNCC 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 206
BalI TGGCCA 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 435
BbvI GCAGC 3 cut(s) 322, 333, 336
BccI CCATC 2 cut(s) 173, 262
BceAI ACGGC 3 cut(s) 42, 80, 421
BcoDI GTCTC 2 cut(s) 65, 320
BfmI CTRYAG 1 cut(s) 350
BisI GCNGC 5 cut(s) 293, 311, 347, 350, 389
BlsI GCNGC 5 cut(s) 294, 312, 348, 351, 390
BmeRI GACNNNNNGTC 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 55
BmsI GCATC 2 cut(s) 67, 333
BpiI GAAGAC 1 cut(s) 435
BpmI CTGGAG 2 cut(s) 284, 344
BpuEI CTTGAG 1 cut(s) 481
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 33
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 317, 347
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 3 cut(s) 137, 371, 398
BseNI ACTGG 1 cut(s) 15
BsePI GCGCGC 2 cut(s) 39, 41
BseRI GAGGAG 1 cut(s) 157
BseXI GCAGC 3 cut(s) 322, 333, 336
Bsh1236I CGCG 6 cut(s) 41, 43, 53, 120, 193, 371
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 57, 272
BsiEI CGRYCG 1 cut(s) 118
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 2 cut(s) 96, 216
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 65, 320
BsmBI CGTCTC 1 cut(s) 65
BsmFI GGGAC 2 cut(s) 96, 216
BsnI GGCC 2 cut(s) 57, 272
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 206
Bsp143I GATC 2 cut(s) 115, 366
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 9 cut(s) 53, 80, 93, 193, 257, 292, 302, 313, 388
BspANI GGCC 2 cut(s) 57, 272
BspCNI CTCAG 3 cut(s) 138, 370, 397
BspFNI CGCG 6 cut(s) 41, 43, 53, 120, 193, 371
BspMAI CTGCAG 1 cut(s) 354
BspTNI GGTCTC 1 cut(s) 320
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 273
BssHII GCGCGC 2 cut(s) 39, 41
BssMI GATC 2 cut(s) 115, 366
BssT1I CCWWGG 1 cut(s) 273
BstC8I GCNNGC 5 cut(s) 41, 43, 55, 214, 255
BstDEI CTNAG 4 cut(s) 109, 146, 357, 384
BstF5I GGATG 1 cut(s) 100
BstFNI CGCG 6 cut(s) 41, 43, 53, 120, 193, 371
BstHHI GCGC 4 cut(s) 41, 43, 45, 53
BstKTI GATC 2 cut(s) 118, 369
BstMAI GTCTC 2 cut(s) 65, 320
BstMBI GATC 2 cut(s) 115, 366
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 3 cut(s) 259, 284, 319
BstSFI CTRYAG 1 cut(s) 350
BstUI CGCG 6 cut(s) 41, 43, 53, 120, 193, 371
BstV1I GCAGC 3 cut(s) 322, 333, 336
BstV2I GAAGAC 1 cut(s) 435
BsuRI GGCC 2 cut(s) 57, 272
BtgZI GCGATG 1 cut(s) 135
BtsCI GGATG 1 cut(s) 100
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 5 cut(s) 41, 43, 55, 214, 255
CaiI CAGNNNCTG 1 cut(s) 346
CfoI GCGC 4 cut(s) 41, 43, 45, 53
Cfr13I GGNCC 1 cut(s) 55
CseI GACGC 3 cut(s) 151, 199, 360
Csp6I GTAC 1 cut(s) 98
CviAII CATG 1 cut(s) 137
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 4 cut(s) 109, 146, 357, 384
DpnI GATC 2 cut(s) 117, 368
DpnII GATC 2 cut(s) 115, 366
DrdI GACNNNNNNGTC 1 cut(s) 196
DriI GACNNNNNGTC 1 cut(s) 436
DseDI GACNNNNNNGTC 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 270
Eam1105I GACNNNNNGTC 1 cut(s) 436
EciI GGCGGA 2 cut(s) 82, 317
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
Esp3I CGTCTC 1 cut(s) 65
FaeI CATG 1 cut(s) 140
FaiI YATR 1 cut(s) 138
FaqI GGGAC 2 cut(s) 96, 216
FatI CATG 1 cut(s) 136
FauI CCCGC 3 cut(s) 46, 73, 306
FblI GTMKAC 1 cut(s) 188
Fnu4HI GCNGC 5 cut(s) 293, 311, 347, 350, 389
FokI GGATG 1 cut(s) 87
Fsp4HI GCNGC 5 cut(s) 293, 311, 347, 350, 389
GlaI GCGC 4 cut(s) 40, 42, 44, 52
GluI GCNGC 5 cut(s) 293, 311, 347, 350, 389
GsuI CTGGAG 2 cut(s) 284, 344
HaeIII GGCC 2 cut(s) 57, 272
HapII CCGG 1 cut(s) 34
HgaI GACGC 3 cut(s) 151, 199, 360
HhaI GCGC 4 cut(s) 41, 43, 45, 53
Hin1II CATG 1 cut(s) 140
Hin6I GCGC 4 cut(s) 39, 41, 43, 51
HinP1I GCGC 4 cut(s) 39, 41, 43, 51
HincII GTYRAC 2 cut(s) 189, 336
HindII GTYRAC 2 cut(s) 189, 336
HpaII CCGG 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 189, 336
Hpy188I TCNGA 9 cut(s) 154, 160, 172, 178, 221, 232, 243, 402, 445
Hpy188III TCNNGA 2 cut(s) 119, 201
Hpy8I GTNNAC 2 cut(s) 189, 336
Hpy99I CGWCG 6 cut(s) 77, 145, 193, 376, 442, 445
HpyAV CCTTC 3 cut(s) 292, 440, 479
HpyCH4V TGCA 3 cut(s) 352, 364, 477
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 284, 319
HpyF3I CTNAG 4 cut(s) 109, 146, 357, 384
Hsp92II CATG 1 cut(s) 140
HspAI GCGC 4 cut(s) 39, 41, 43, 51
Kzo9I GATC 2 cut(s) 115, 366
LmnI GCTCC 5 cut(s) 36, 170, 250, 259, 449
LpnPI CCDG 4 cut(s) 28, 47, 248, 308
Lsp1109I GCAGC 3 cut(s) 322, 333, 336
LweI GCATC 2 cut(s) 67, 333
MalI GATC 2 cut(s) 117, 368
MboI GATC 2 cut(s) 115, 366
MboII GAAGA 3 cut(s) 140, 437, 440
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 1 cut(s) 478
MluNI TGGCCA 1 cut(s) 272
MmeI TCCRAC 3 cut(s) 177, 195, 423
MnlI CCTC 4 cut(s) 178, 202, 277, 476
Mox20I TGGCCA 1 cut(s) 272
MscI TGGCCA 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 272
MspA1I CMGCKG 2 cut(s) 313, 388
MspI CCGG 1 cut(s) 34
MvnI CGCG 6 cut(s) 41, 43, 53, 120, 193, 371
MwoI GCNNNNNNNGC 3 cut(s) 259, 284, 319
NdeII GATC 2 cut(s) 115, 366
NlaIII CATG 1 cut(s) 140
NruI TCGCGA 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 206
PauI GCGCGC 2 cut(s) 39, 41
PcsI WCGNNNNNNNCGW 3 cut(s) 16, 403, 429
PfeI GAWTC 2 cut(s) 131, 238
PkrI GCNGC 5 cut(s) 294, 312, 348, 351, 390
Ple19I CGATCG 1 cut(s) 118
PspPI GGNCC 1 cut(s) 55
PspXI VCTCGAGB 1 cut(s) 206
PstI CTGCAG 1 cut(s) 354
PstNI CAGNNNCTG 1 cut(s) 346
PteI GCGCGC 2 cut(s) 39, 41
PvuI CGATCG 1 cut(s) 118
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
SalI GTCGAC 1 cut(s) 187
SatI GCNGC 5 cut(s) 293, 311, 347, 350, 389
Sau3AI GATC 2 cut(s) 115, 366
Sau96I GGNCC 1 cut(s) 55
SetI ASST 5 cut(s) 167, 218, 264, 324, 454
SfaNI GCATC 2 cut(s) 67, 333
SfcI CTRYAG 1 cut(s) 350
Sfr274I CTCGAG 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 2 cut(s) 206, 460
SmoI CTYRAG 2 cut(s) 206, 460
Sse9I AATT 1 cut(s) 478
SsiI CCGC 9 cut(s) 53, 80, 93, 193, 257, 292, 302, 313, 388
StyI CCWWGG 1 cut(s) 273
TaqI TCGA 8 cut(s) 19, 75, 114, 188, 207, 236, 318, 423
TaqII GACCGA 1 cut(s) 344
TasI AATT 1 cut(s) 478
TauI GCSGC 2 cut(s) 295, 391
TfiI GAWTC 2 cut(s) 131, 238
TseI GCWGC 3 cut(s) 310, 346, 349
TspGWI ACGGA 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.