Rorug04G0394400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017934
Physical Location & Seq
Reverse (-)
56060084 .. 56061781
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0394400.1

Sequence Viewer

Length: 642 bp
ATGGTTGAATTGCGTTTACTGGTTGCTGCTGCTGTGATCCTAGGGTTTCTATTCTCCACATCCCAATCTATCCGCTTCGAGCTCCAATCGGGTCACACCAAATGCATCGTCGAAGACATGAAGGCCAATGCCATGACCGTCGGAAAGTACTCCGTCGTCAACCCCGACGAAGGCCAGCCCCTGCCGCCCTCACACAAGCTCACCGTTAGGGTGACTTCGAGCTATGGGAATAGCCATCACTACTCGGAGCTTGTGGAATCGGGTCAGTTTGCTTTTCAGGCGGTGGAGGCCGGCGATTACATGGCGTGTTTCTGGGCGCCCGATCACAAGCCCCCGACCACGTTGACCATTGAGTTTGATTGGAAGACGGGTGTGGCGGCCAAGGACTGGTCCAATGTGGCCAAGAAAGGCTCTGTTGATGTCATGGAATTAGAGCTTAAGAAGTTGTTCGACACTGTTACTTCAATTCACGAGGAGATGACCTACCTCCGTGAACGGGAAGAAGAAATGCAGGATCTTAATATAGCAACCAACTCCAAAATGGCCTGGTTGAGTTTTCTTTCGCTTTTTATTTGCTTATCGGTGGCAGGTTTGCAATGGTGGCACTTGAAGACCTTTTTCCAGAAAAAGAAGCTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

24.13

Weight (kDa)

5.98

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 24 - 208 8.5e-50 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017934)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22160 AT4G22160
fragaria_vesca FvH4_5g10500
malus_domestica MD10G1326900.v1.1
prunus_persica Prupe.4G015100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002091
rosa_laevigata RLG00000030993
rosa_multiflora Rmu_co8270171.1_g000001
rosa_rugosa Rorug04G0394400
rosa_samantha Rh5BG019200
rosa_wichuraiana Rw5G001550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 578
AccB1I GGYRCC 1 cut(s) 316
AccB7I CCANNNNNTGG 1 cut(s) 387
AciI CCGC 4 cut(s) 73, 185, 281, 377
AclWI GGATC 2 cut(s) 31, 522
AcoI YGGCCR 2 cut(s) 378, 399
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 3 cut(s) 170, 387, 496
AflII CTTAAG 1 cut(s) 437
AgsI TTSAA 3 cut(s) 8, 465, 610
AjnI CCWGG 1 cut(s) 545
AluBI AGCT 6 cut(s) 82, 199, 222, 250, 436, 634
AluI AGCT 6 cut(s) 82, 199, 222, 250, 436, 634
Alw21I GWGCWC 1 cut(s) 84
AlwI GGATC 2 cut(s) 31, 522
AlwNI CAGNNNCTG 1 cut(s) 181
AoxI GGCC 6 cut(s) 123, 172, 288, 378, 399, 543
ApeKI GCWGC 2 cut(s) 26, 29
ArsI GACNNNNNNTTYG 2 cut(s) 93, 125
Asp700I GAANNNNTTC 1 cut(s) 446
AspA2I CCTAGG 1 cut(s) 40
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 390
AsuHPI GGTGA 2 cut(s) 193, 223
AvaII GGWCC 1 cut(s) 390
AvrII CCTAGG 1 cut(s) 40
BalI TGGCCA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 316
BanII GRGCYC 1 cut(s) 84
BauI CACGAG 1 cut(s) 470
BbsI GAAGAC 3 cut(s) 120, 371, 617
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 13, 16
BccI CCATC 1 cut(s) 243
BciT130I CCWGG 1 cut(s) 547
BfaI CTAG 1 cut(s) 41
BfoI RGCGCY 1 cut(s) 320
BfrI CTTAAG 1 cut(s) 437
BfuAI ACCTGC 1 cut(s) 578
BisI GCNGC 4 cut(s) 27, 30, 185, 378
BlnI CCTAGG 1 cut(s) 40
BlsI GCNGC 4 cut(s) 28, 31, 186, 379
BmcAI AGTACT 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 547
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 547
BmsI GCATC 1 cut(s) 114
BpiI GAAGAC 3 cut(s) 120, 371, 617
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 40, 381
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 387, 496
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 2 cut(s) 24, 392
Bse3DI GCAATG 1 cut(s) 602
BseBI CCWGG 1 cut(s) 547
BseDI CCNNGG 2 cut(s) 40, 381
BseGI GGATG 1 cut(s) 59
BseLI CCNNNNNNNGG 3 cut(s) 170, 387, 496
BseMI GCAATG 1 cut(s) 602
BseNI ACTGG 2 cut(s) 24, 392
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 2 cut(s) 13, 16
BshFI GGCC 6 cut(s) 125, 174, 290, 380, 401, 545
BshNI GGYRCC 1 cut(s) 316
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 1 cut(s) 291
BslI CCNNNNNNNGG 3 cut(s) 170, 387, 496
BsnI GGCC 6 cut(s) 125, 174, 290, 380, 401, 545
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 3 cut(s) 36, 322, 514
BspACI CCGC 4 cut(s) 73, 185, 281, 377
BspANI GGCC 6 cut(s) 125, 174, 290, 380, 401, 545
BspLI GGNNCC 1 cut(s) 318
BspMI ACCTGC 1 cut(s) 578
BspPI GGATC 2 cut(s) 31, 522
BspT107I GGYRCC 1 cut(s) 316
BspTI CTTAAG 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 602
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 2 cut(s) 24, 392
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 40, 381
BssMI GATC 3 cut(s) 36, 322, 514
BssNI GRCGYC 1 cut(s) 317
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 2 cut(s) 40, 381
Bst2BI CACGAG 1 cut(s) 470
Bst2UI CCWGG 1 cut(s) 547
Bst4CI ACNGT 3 cut(s) 139, 205, 457
BstACI GRCGYC 1 cut(s) 317
BstAFI CTTAAG 1 cut(s) 437
BstC8I GCNNGC 2 cut(s) 176, 292
BstF5I GGATG 1 cut(s) 59
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 3 cut(s) 39, 325, 517
BstMBI GATC 3 cut(s) 36, 322, 514
BstMWI GCNNNNNNNGC 4 cut(s) 184, 278, 287, 601
BstNI CCWGG 1 cut(s) 547
BstSCI CCNGG 1 cut(s) 545
BstV1I GCAGC 2 cut(s) 13, 16
BstV2I GAAGAC 3 cut(s) 120, 371, 617
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 6 cut(s) 125, 174, 290, 380, 401, 545
BtsCI GGATG 1 cut(s) 59
BtsIMutI CAGTG 1 cut(s) 453
BveI ACCTGC 1 cut(s) 578
Cac8I GCNNGC 2 cut(s) 176, 292
CaiI CAGNNNCTG 1 cut(s) 181
CfoI GCGC 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 390
Csp6I GTAC 1 cut(s) 148
CviAII CATG 4 cut(s) 118, 133, 301, 424
CviQI GTAC 1 cut(s) 148
DinI GGCGCC 1 cut(s) 318
DpnI GATC 3 cut(s) 38, 324, 516
DpnII GATC 3 cut(s) 36, 322, 514
EaeI YGGCCR 2 cut(s) 378, 399
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 2 cut(s) 40, 381
Eco24I GRGCYC 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 390
Eco53kI GAGCTC 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 545
EcoT14I CCWWGG 2 cut(s) 40, 381
EcoT22I ATGCAT 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 84
EgeI GGCGCC 1 cut(s) 318
EheI GGCGCC 1 cut(s) 318
ErhI CCWWGG 2 cut(s) 40, 381
FaeI CATG 4 cut(s) 121, 136, 304, 427
FaiI YATR 6 cut(s) 119, 134, 225, 302, 425, 524
FatI CATG 4 cut(s) 117, 132, 300, 423
Fnu4HI GCNGC 4 cut(s) 27, 30, 185, 378
FokI GGATG 1 cut(s) 46
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 4 cut(s) 27, 30, 185, 378
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 318
GluI GCNGC 4 cut(s) 27, 30, 185, 378
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 6 cut(s) 125, 174, 290, 380, 401, 545
HapII CCGG 1 cut(s) 291
HhaI GCGC 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 4 cut(s) 121, 136, 304, 427
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HincII GTYRAC 2 cut(s) 160, 345
HindII GTYRAC 2 cut(s) 160, 345
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 1 cut(s) 257
HpaII CCGG 1 cut(s) 291
HphI GGTGA 2 cut(s) 193, 223
Hpy166II GTNNAC 4 cut(s) 17, 160, 345, 494
Hpy188I TCNGA 2 cut(s) 143, 247
Hpy188III TCNNGA 2 cut(s) 470, 622
Hpy8I GTNNAC 4 cut(s) 17, 160, 345, 494
Hpy99I CGWCG 4 cut(s) 113, 143, 158, 170
HpyAV CCTTC 2 cut(s) 115, 164
HpyCH4III ACNGT 3 cut(s) 139, 205, 457
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 3 cut(s) 105, 511, 595
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 278, 287, 601
HpySE526I ACGT 1 cut(s) 341
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 4 cut(s) 121, 136, 304, 427
HspAI GCGC 1 cut(s) 317
KasI GGCGCC 1 cut(s) 316
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
Kzo9I GATC 3 cut(s) 36, 322, 514
LmnI GCTCC 2 cut(s) 87, 247
Lsp1109I GCAGC 2 cut(s) 13, 16
LweI GCATC 1 cut(s) 114
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 3 cut(s) 92, 211, 457
MalI GATC 3 cut(s) 38, 324, 516
MboI GATC 3 cut(s) 36, 322, 514
MboII GAAGA 5 cut(s) 125, 376, 512, 515, 622
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 84
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 3 cut(s) 8, 428, 465
MluNI TGGCCA 1 cut(s) 401
Mly113I GGCGCC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 4 cut(s) 199, 280, 466, 497
Mox20I TGGCCA 1 cut(s) 401
Mph1103I ATGCAT 1 cut(s) 107
MroNI GCCGGC 1 cut(s) 290
MroXI GAANNNNTTC 1 cut(s) 446
MscI TGGCCA 1 cut(s) 401
MseI TTAA 2 cut(s) 438, 519
Msp20I TGGCCA 1 cut(s) 401
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 547
MvaI CCWGG 1 cut(s) 547
MwoI GCNNNNNNNGC 4 cut(s) 184, 278, 287, 601
NaeI GCCGGC 1 cut(s) 292
NarI GGCGCC 1 cut(s) 317
NdeII GATC 3 cut(s) 36, 322, 514
NgoMIV GCCGGC 1 cut(s) 290
NlaIII CATG 4 cut(s) 121, 136, 304, 427
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 92, 211
NsiI ATGCAT 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 1 cut(s) 257
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 4 cut(s) 28, 31, 186, 379
PluTI GGCGCC 1 cut(s) 320
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 545
PspGI CCWGG 1 cut(s) 545
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 438, 519
SatI GCNGC 4 cut(s) 27, 30, 185, 378
Sau3AI GATC 3 cut(s) 36, 322, 514
Sau96I GGNCC 1 cut(s) 390
ScaI AGTACT 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 547
SduI GDGCHC 1 cut(s) 84
SfaNI GCATC 1 cut(s) 114
SfoI GGCGCC 1 cut(s) 318
SinI GGWCC 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 3 cut(s) 8, 428, 465
SsiI CCGC 4 cut(s) 73, 185, 281, 377
SspDI GGCGCC 1 cut(s) 316
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 545
StyI CCWWGG 2 cut(s) 40, 381
TaaI ACNGT 3 cut(s) 139, 205, 457
TaiI ACGT 1 cut(s) 344
TaqI TCGA 4 cut(s) 78, 111, 218, 450
TasI AATT 3 cut(s) 8, 428, 465
TatI WGTACW 1 cut(s) 147
TauI GCSGC 2 cut(s) 187, 380
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 2 cut(s) 438, 519
Tru9I TTAA 2 cut(s) 438, 519
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 2 cut(s) 92, 211
TseI GCWGC 2 cut(s) 26, 29
Tsp45I GTSAC 2 cut(s) 92, 211
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 2 cut(s) 142, 479
TspRI CASTG 1 cut(s) 460
Van91I CCANNNNNTGG 1 cut(s) 387
Vha464I CTTAAG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 390
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XmaJI CCTAGG 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 446
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 149
Zsp2I ATGCAT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.