MD10G1341800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0017258
Physical Location & Seq
Forward (+)
41656590 .. 41658350
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1341800.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGAGCGTGACAGCAAAAAGAGAGTAAGAGAAGGAGATGAAGACCACAACGATTCTTCTTCTTCTTCTTCTTGTTATTCTTTTTCAGAGAAGCATGCGAAGACAAATTTAGTAGATCCTAACTGTGGTATTTTGGACTTCAAAAACGACCTTGAATACAACAGCAGCGGCAGCAGCACTAGTAGTAATAGTAGCAGTTATAAGACATCAGATTCGGATTCCAACTTGGCATTAGGAGTATTTGATTTTCCGTGGCTGAAAGATAGTGTAATATCCAAACCAGAGGACTTGAATTTTGAAGACTCATTTTCATCACCACTGGCAGTACTGGATGTCGACACATCCACCTTGACTACCAGTAGTACTACTCCTGGACATGGTAATTTTTCATCACCACTGGCAGTACTGGATGATCAATTTCCTTTCTCCGGGCAGTGCTTCTATCATGATCAGACTGATCATCCTGATGCACTCCTGGACTTTCCAAAGGACAAGCTTGATATAGATTTGTGGCGACCTCGAGATCTTAATCAAGATGATGGGTCGAAGACTGAGGCCGGGGAAGATTGCATTTGGAGTTCCCTGCTCAATCAGCCTCTTCAACAGCCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.59

Weight (kDa)

4.27

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00131
malus_domestica MD10G1341800.v1.1
prunus_persica Prupe.4G001400_v2.0.a1
pyrus_communis pycom10g29110
rosa_chinensis RchiOBHm_Chr5g0000161
rosa_laevigata RLG00000030846
rosa_roxburghii Rroxscaffold_1G00075930
rosa_rugosa Rorug04G0382000
rosa_samantha Rh5AG001100 Rh5BG002500 Rh5CG001300 Rh5DG001200
rosa_wichuraiana Rw5G000150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 201
AccI GTMKAC 1 cut(s) 336
AciI CCGC 1 cut(s) 168
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 2 cut(s) 106, 292
AfaI GTAC 3 cut(s) 327, 364, 405
AfiI CCNNNNNNNGG 3 cut(s) 125, 377, 428
AgsI TTSAA 5 cut(s) 142, 155, 292, 299, 602
AhlI ACTAGT 1 cut(s) 179
AjnI CCWGG 2 cut(s) 370, 474
AluBI AGCT 1 cut(s) 496
AluI AGCT 1 cut(s) 496
AlwI GGATC 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 519
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 3 cut(s) 165, 171, 174
ApoI RAATTY 2 cut(s) 106, 292
ArsI GACNNNNNNTTYG 4 cut(s) 196, 228, 278, 310
AsuC2I CCSGG 2 cut(s) 430, 559
AsuHPI GGTGA 2 cut(s) 306, 384
AvaI CYCGRG 1 cut(s) 519
BbsI GAAGAC 4 cut(s) 48, 107, 306, 554
BbvI GCAGC 3 cut(s) 177, 183, 186
BccI CCATC 1 cut(s) 533
BciT130I CCWGG 2 cut(s) 372, 476
BclI TGATCA 3 cut(s) 412, 448, 457
BcnI CCSGG 2 cut(s) 430, 559
BcuI ACTAGT 1 cut(s) 179
BfaI CTAG 1 cut(s) 180
BglII AGATCT 1 cut(s) 523
BisI GCNGC 4 cut(s) 166, 169, 172, 175
BlsI GCNGC 4 cut(s) 167, 170, 173, 176
BmcAI AGTACT 3 cut(s) 327, 364, 405
Bme1390I CCNGG 4 cut(s) 372, 430, 476, 559
BmeT110I CYCGRG 1 cut(s) 519
BmrFI CCNGG 4 cut(s) 372, 430, 476, 559
BmsI GCATC 1 cut(s) 457
BpiI GAAGAC 4 cut(s) 48, 107, 306, 554
BpuMI CCSGG 2 cut(s) 430, 559
BsaBI GATNNNNATC 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 251, 558
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 377, 428
Bse1I ACTGG 5 cut(s) 324, 333, 357, 402, 411
Bse8I GATNNNNATC 1 cut(s) 528
BseBI CCWGG 2 cut(s) 372, 476
BseDI CCNNGG 2 cut(s) 251, 558
BseGI GGATG 4 cut(s) 337, 341, 415, 460
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 3 cut(s) 125, 377, 428
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 5 cut(s) 324, 333, 357, 402, 411
BseXI GCAGC 3 cut(s) 177, 183, 186
BshFI GGCC 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 519
BsiSI CCGG 2 cut(s) 429, 558
BslI CCNNNNNNNGG 3 cut(s) 125, 377, 428
BsnI GGCC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 519
Bsp143I GATC 5 cut(s) 115, 412, 448, 457, 523
BspACI CCGC 1 cut(s) 168
BspANI GGCC 1 cut(s) 557
BspCNI CTCAG 1 cut(s) 544
BspHI TCATGA 1 cut(s) 445
BspPI GGATC 1 cut(s) 110
BsrI ACTGG 5 cut(s) 324, 333, 357, 402, 411
BssECI CCNNGG 2 cut(s) 251, 558
BssMI GATC 5 cut(s) 115, 412, 448, 457, 523
Bst2UI CCWGG 2 cut(s) 372, 476
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 552
BstDSI CCRYGG 1 cut(s) 251
BstF5I GGATG 4 cut(s) 337, 341, 415, 460
BstKTI GATC 5 cut(s) 118, 415, 451, 460, 526
BstMBI GATC 5 cut(s) 115, 412, 448, 457, 523
BstMWI GCNNNNNNNGC 3 cut(s) 171, 174, 592
BstNI CCWGG 2 cut(s) 372, 476
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 4 cut(s) 370, 428, 474, 557
BstV1I GCAGC 3 cut(s) 177, 183, 186
BstV2I GAAGAC 4 cut(s) 48, 107, 306, 554
BstX2I RGATCY 2 cut(s) 115, 523
BstYI RGATCY 2 cut(s) 115, 523
BsuRI GGCC 1 cut(s) 557
BtgI CCRYGG 1 cut(s) 251
BtsCI GGATG 4 cut(s) 337, 341, 415, 460
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 3 cut(s) 317, 395, 440
Cac8I GCNNGC 1 cut(s) 96
CciI TCATGA 1 cut(s) 445
Csp6I GTAC 3 cut(s) 326, 363, 404
CviAII CATG 3 cut(s) 95, 377, 446
CviJI RGCY 5 cut(s) 256, 496, 557, 595, 607
CviKI_1 RGCY 5 cut(s) 256, 496, 557, 595, 607
CviQI GTAC 3 cut(s) 326, 363, 404
DdeI CTNAG 1 cut(s) 552
DpnI GATC 5 cut(s) 117, 414, 450, 459, 525
DpnII GATC 5 cut(s) 115, 412, 448, 457, 523
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 519
EcoRII CCWGG 2 cut(s) 370, 474
FaeI CATG 3 cut(s) 98, 380, 449
FaiI YATR 5 cut(s) 96, 201, 378, 447, 503
FatI CATG 3 cut(s) 94, 376, 445
FbaI TGATCA 3 cut(s) 412, 448, 457
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 4 cut(s) 166, 169, 172, 175
FokI GGATG 4 cut(s) 328, 344, 422, 447
Fsp4HI GCNGC 4 cut(s) 166, 169, 172, 175
FspBI CTAG 1 cut(s) 180
GluI GCNGC 4 cut(s) 166, 169, 172, 175
HaeIII GGCC 1 cut(s) 557
HapII CCGG 2 cut(s) 429, 558
Hin1II CATG 3 cut(s) 98, 380, 449
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 4 cut(s) 53, 212, 218, 302
HpaII CCGG 2 cut(s) 429, 558
HphI GGTGA 2 cut(s) 306, 384
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 4 cut(s) 88, 211, 217, 453
Hpy188III TCNNGA 4 cut(s) 446, 464, 521, 533
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 1 cut(s) 26
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4V TGCA 2 cut(s) 470, 570
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 174, 592
HpyF3I CTNAG 1 cut(s) 552
Hsp92II CATG 3 cut(s) 98, 380, 449
Ksp22I TGATCA 3 cut(s) 412, 448, 457
Kzo9I GATC 5 cut(s) 115, 412, 448, 457, 523
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 3 cut(s) 177, 183, 186
LweI GCATC 1 cut(s) 457
MaeI CTAG 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 8
MalI GATC 5 cut(s) 117, 414, 450, 459, 525
MboI GATC 5 cut(s) 115, 412, 448, 457, 523
MflI RGATCY 2 cut(s) 115, 523
MluCI AATT 4 cut(s) 106, 292, 382, 416
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 4 cut(s) 277, 528, 547, 606
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 1 cut(s) 465
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 2 cut(s) 429, 558
MspR9I CCNGG 4 cut(s) 372, 430, 476, 559
MvaI CCWGG 2 cut(s) 372, 476
MwoI GCNNNNNNNGC 3 cut(s) 171, 174, 592
NciI CCSGG 2 cut(s) 430, 559
NdeII GATC 5 cut(s) 115, 412, 448, 457, 523
NlaIII CATG 3 cut(s) 98, 380, 449
NmuCI GTSAC 1 cut(s) 8
NspI RCATGY 1 cut(s) 98
PaeI GCATGC 1 cut(s) 98
PaeR7I CTCGAG 1 cut(s) 519
PagI TCATGA 1 cut(s) 445
PfeI GAWTC 3 cut(s) 53, 212, 218
PfoI TCCNGGA 2 cut(s) 370, 474
PkrI GCNGC 4 cut(s) 167, 170, 173, 176
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PsiI TTATAA 1 cut(s) 201
Psp6I CCWGG 2 cut(s) 370, 474
PspGI CCWGG 2 cut(s) 370, 474
PsuI RGATCY 2 cut(s) 115, 523
RsaI GTAC 3 cut(s) 327, 364, 405
RsaNI GTAC 3 cut(s) 326, 363, 404
RseI CAYNNNNRTG 1 cut(s) 465
SalI GTCGAC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 4 cut(s) 166, 169, 172, 175
Sau3AI GATC 5 cut(s) 115, 412, 448, 457, 523
ScaI AGTACT 3 cut(s) 327, 364, 405
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 4 cut(s) 372, 430, 476, 559
SetI ASST 4 cut(s) 153, 350, 498, 520
SfaNI GCATC 1 cut(s) 457
Sfr274I CTCGAG 1 cut(s) 519
SlaI CTCGAG 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
SpeI ACTAGT 1 cut(s) 179
SphI GCATGC 1 cut(s) 98
Sse9I AATT 4 cut(s) 106, 292, 382, 416
SsiI CCGC 1 cut(s) 168
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 4 cut(s) 370, 428, 474, 557
TaaI ACNGT 1 cut(s) 125
TaqI TCGA 3 cut(s) 336, 520, 545
TasI AATT 4 cut(s) 106, 292, 382, 416
TatI WGTACW 3 cut(s) 325, 362, 403
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 3 cut(s) 53, 212, 218
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 3 cut(s) 324, 402, 440
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 3 cut(s) 165, 171, 174
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 3 cut(s) 54, 300, 378
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 3 cut(s) 324, 402, 440
XapI RAATTY 2 cut(s) 106, 292
XceI RCATGY 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 519
XmiI GTMKAC 1 cut(s) 336
XspI CTAG 1 cut(s) 180
ZrmI AGTACT 3 cut(s) 327, 364, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.