Prupe.4G001400_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017258
Physical Location & Seq
Reverse (-)
139369 .. 141582
2214 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G001400.1

Sequence Viewer

Length: 633 bp
ATGGAGCGTGACAGCAAGAAGAGAGTAAGAGAAGGAGACGAAGGCCACAATAATTCTTCTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAGAGAGAGAGAAGCATGTAAAGACAAAAGATGGTCCCATTAATTATGGTATTTTGGAGTTGAAAAATGACGTACTCGAATACAAGGACAGCAGCAGCAGTACCAGTAGCTGTTGTAAGACATCATCAGAATCCCACTGCGCATTAGGAGTATTTGATTTTCCGTGGCTGAAAGATGGTATAATCTCCCAATCGGAGGAGTGGATTAATTTTGAGGACGTCTTCTCATCACCAGTACTGGCACTGGATGACACACCCACCTTTACTACTAGTAGTAATCCTGGTCATGGTATAGGTATTGATGATCAATTTTCTTTCTCCGCGGCGGCGGGGCGGTGTTTCTATCAGACTCATGATCAATCCGATCATCAGGAACTACTCCTAGACTTTCCTCCAGCTAAGGATAAGCTTGAGGAAGATGCACGGCGAAGTCCTCGAGATCTTAATCAAGATGATGATGGCTTGGAGAATGAGGGTGCGGATTGCATTTGGACCTTCCTGCTCAATCAGCCGCTTCATCAACAGCCCAACCCAACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

23.34

Weight (kDa)

4.56

Isoelectric Point (pI)

60.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00131
malus_domestica MD10G1341800.v1.1
prunus_persica Prupe.4G001400_v2.0.a1
pyrus_communis pycom10g29110
rosa_chinensis RchiOBHm_Chr5g0000161
rosa_laevigata RLG00000030846
rosa_roxburghii Rroxscaffold_1G00075930
rosa_rugosa Rorug04G0382000
rosa_samantha Rh5AG001100 Rh5BG002500 Rh5CG001300 Rh5DG001200
rosa_wichuraiana Rw5G000150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
Acc16I TGCGCA 1 cut(s) 232
AccII CGCG 1 cut(s) 413
AciI CCGC 7 cut(s) 411, 413, 416, 419, 424, 569, 602
AcuI CTGAAG 1 cut(s) 75
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 3 cut(s) 165, 193, 327
AfiI CCNNNNNNNGG 2 cut(s) 286, 377
AgsI TTSAA 1 cut(s) 154
AhlI ACTAGT 1 cut(s) 359
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 3 cut(s) 201, 488, 499
AluI AGCT 3 cut(s) 201, 488, 499
Alw26I GTCTC 1 cut(s) 30
AlwNI CAGNNNCTG 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 525
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 2 cut(s) 183, 186
AseI ATTAAT 2 cut(s) 132, 297
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 125, 582
AsuHPI GGTGA 1 cut(s) 312
AvaI CYCGRG 1 cut(s) 525
AvaII GGWCC 2 cut(s) 125, 582
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 2 cut(s) 195, 198
BccI CCATC 3 cut(s) 116, 260, 542
BceAI ACGGC 1 cut(s) 530
BciT130I CCWGG 1 cut(s) 372
BclI TGATCA 2 cut(s) 394, 445
BcoDI GTCTC 1 cut(s) 30
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 2 cut(s) 360, 473
BglII AGATCT 1 cut(s) 529
BisI GCNGC 5 cut(s) 184, 187, 414, 417, 602
BlsI GCNGC 5 cut(s) 185, 188, 415, 418, 603
BmcAI AGTACT 1 cut(s) 327
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 2 cut(s) 125, 582
BmeT110I CYCGRG 1 cut(s) 525
BmgT120I GGNCC 2 cut(s) 125, 582
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 1 cut(s) 499
BpiI GAAGAC 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 468
Bpu10I CCTNAGC 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 521
BsaBI GATNNNNATC 1 cut(s) 534
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 254, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 377
Bse1I ACTGG 4 cut(s) 195, 323, 333, 339
Bse8I GATNNNNATC 1 cut(s) 534
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 254, 411
BseGI GGATG 1 cut(s) 343
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 2 cut(s) 286, 377
BseNI ACTGG 4 cut(s) 195, 323, 333, 339
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 2 cut(s) 195, 198
Bsh1236I CGCG 1 cut(s) 413
BshFI GGCC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 525
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 2 cut(s) 286, 377
BsmAI GTCTC 1 cut(s) 30
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 111
BsnI GGCC 1 cut(s) 45
BsoBI CYCGRG 1 cut(s) 525
Bsp143I GATC 4 cut(s) 394, 445, 454, 529
BspACI CCGC 7 cut(s) 411, 413, 416, 419, 424, 569, 602
BspANI GGCC 1 cut(s) 45
BspFNI CGCG 1 cut(s) 413
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 1 cut(s) 127
BsrI ACTGG 4 cut(s) 195, 323, 333, 339
BssECI CCNNGG 2 cut(s) 254, 411
BssMI GATC 4 cut(s) 394, 445, 454, 529
BssNI GRCGYC 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 372
Bst6I CTCTTC 1 cut(s) 14
BstACI GRCGYC 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 489
BstDSI CCRYGG 2 cut(s) 254, 411
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 1 cut(s) 413
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 4 cut(s) 397, 448, 457, 532
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 4 cut(s) 394, 445, 454, 529
BstMWI GCNNNNNNNGC 1 cut(s) 598
BstNI CCWGG 1 cut(s) 372
BstNSI RCATGY 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 370
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 2 cut(s) 195, 198
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 1 cut(s) 45
BtgI CCRYGG 2 cut(s) 254, 411
BtsCI GGATG 1 cut(s) 343
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 2 cut(s) 226, 332
CaiI CAGNNNCTG 1 cut(s) 201
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 125, 582
Cfr42I CCGCGG 1 cut(s) 414
Csp6I GTAC 3 cut(s) 164, 192, 326
CviAII CATG 3 cut(s) 107, 377, 443
CviJI RGCY 8 cut(s) 45, 201, 259, 488, 499, 552, 601, 616
CviKI_1 RGCY 8 cut(s) 45, 201, 259, 488, 499, 552, 601, 616
CviQI GTAC 3 cut(s) 164, 192, 326
DdeI CTNAG 1 cut(s) 489
DpnI GATC 4 cut(s) 396, 447, 456, 531
DpnII GATC 4 cut(s) 394, 445, 454, 529
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco47I GGWCC 2 cut(s) 125, 582
Eco57I CTGAAG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 370
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 3 cut(s) 110, 380, 446
FaiI YATR 6 cut(s) 108, 138, 272, 378, 383, 444
FaqI GGGAC 1 cut(s) 111
FatI CATG 3 cut(s) 106, 376, 442
FauI CCCGC 1 cut(s) 412
FbaI TGATCA 2 cut(s) 394, 445
Fnu4HI GCNGC 5 cut(s) 184, 187, 414, 417, 602
FokI GGATG 1 cut(s) 350
Fsp4HI GCNGC 5 cut(s) 184, 187, 414, 417, 602
FspBI CTAG 2 cut(s) 360, 473
FspI TGCGCA 1 cut(s) 232
GlaI GCGC 1 cut(s) 232
GluI GCNGC 5 cut(s) 184, 187, 414, 417, 602
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 1 cut(s) 45
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 3 cut(s) 110, 380, 446
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 2 cut(s) 221, 439
HphI GGTGA 1 cut(s) 312
Hpy188I TCNGA 5 cut(s) 94, 220, 286, 438, 454
Hpy188III TCNNGA 4 cut(s) 443, 461, 527, 539
HpyAV CCTTC 4 cut(s) 26, 35, 72, 595
HpyCH4IV ACGT 2 cut(s) 162, 309
HpyCH4V TGCA 2 cut(s) 512, 576
HpyF10VI GCNNNNNNNGC 1 cut(s) 598
HpyF3I CTNAG 1 cut(s) 489
HpySE526I ACGT 2 cut(s) 162, 309
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 3 cut(s) 110, 380, 446
HspAI GCGC 1 cut(s) 231
Ksp22I TGATCA 2 cut(s) 394, 445
KspI CCGCGG 1 cut(s) 414
Kzo9I GATC 4 cut(s) 394, 445, 454, 529
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 208, 314, 320, 336, 357, 384, 446, 498, 602
Lsp1109I GCAGC 2 cut(s) 195, 198
LweI GCATC 1 cut(s) 499
MaeI CTAG 2 cut(s) 360, 473
MaeII ACGT 2 cut(s) 162, 309
MaeIII GTNAC 1 cut(s) 8
MalI GATC 4 cut(s) 396, 447, 456, 531
MboI GATC 4 cut(s) 394, 445, 454, 529
MflI RGATCY 1 cut(s) 529
MluCI AATT 5 cut(s) 52, 133, 298, 398, 628
MlyI GAGTC 1 cut(s) 433
MnlI CCTC 6 cut(s) 280, 298, 492, 496, 534, 556
MseI TTAA 4 cut(s) 132, 297, 534, 631
MspA1I CMGCKG 1 cut(s) 413
MspR9I CCNGG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 1 cut(s) 598
NdeII GATC 4 cut(s) 394, 445, 454, 529
NlaIII CATG 3 cut(s) 110, 380, 446
NlaIV GGNNCC 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 8
NsbI TGCGCA 1 cut(s) 232
NspI RCATGY 1 cut(s) 110
PaeR7I CTCGAG 1 cut(s) 525
PagI TCATGA 1 cut(s) 442
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 5 cut(s) 185, 188, 415, 418, 603
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
PshBI ATTAAT 2 cut(s) 132, 297
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 2 cut(s) 125, 582
PstNI CAGNNNCTG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 3 cut(s) 165, 193, 327
RsaNI GTAC 3 cut(s) 164, 192, 326
SacII CCGCGG 1 cut(s) 414
SaqAI TTAA 4 cut(s) 132, 297, 534, 631
SatI GCNGC 5 cut(s) 184, 187, 414, 417, 602
Sau3AI GATC 4 cut(s) 394, 445, 454, 529
Sau96I GGNCC 2 cut(s) 125, 582
ScaI AGTACT 1 cut(s) 327
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 1 cut(s) 372
SetI ASST 8 cut(s) 165, 203, 312, 353, 388, 490, 501, 587
SfaNI GCATC 1 cut(s) 499
Sfr274I CTCGAG 1 cut(s) 525
Sfr303I CCGCGG 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 414
SinI GGWCC 2 cut(s) 125, 582
SlaI CTCGAG 1 cut(s) 525
SmlI CTYRAG 2 cut(s) 500, 525
SmoI CTYRAG 2 cut(s) 500, 525
SpeI ACTAGT 1 cut(s) 359
Sse9I AATT 5 cut(s) 52, 133, 298, 398, 628
SsiI CCGC 7 cut(s) 411, 413, 416, 419, 424, 569, 602
SspMI CTAG 2 cut(s) 360, 473
StyD4I CCNGG 1 cut(s) 370
TaiI ACGT 2 cut(s) 165, 312
TaqI TCGA 2 cut(s) 168, 526
TasI AATT 5 cut(s) 52, 133, 298, 398, 628
TatI WGTACW 1 cut(s) 325
TauI GCSGC 3 cut(s) 416, 419, 604
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 4 cut(s) 132, 297, 534, 631
Tru9I TTAA 4 cut(s) 132, 297, 534, 631
TscAI CASTG 2 cut(s) 233, 339
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 2 cut(s) 183, 186
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 1 cut(s) 596
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 2 cut(s) 233, 339
VpaK11BI GGWCC 2 cut(s) 125, 582
VspI ATTAAT 2 cut(s) 132, 297
XceI RCATGY 1 cut(s) 110
XhoI CTCGAG 1 cut(s) 525
XspI CTAG 2 cut(s) 360, 473
ZraI GACGTC 1 cut(s) 310
ZrmI AGTACT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.