MD11G1001000.v1.1

Nuclear pore complex protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009160
Physical Location & Seq
Forward (+)
136707 .. 149465
12759 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1001000.v1.1.491

Sequence Viewer

Length: 5367 bp
ATGACAGATAGTGGGAAAGTGACTCGAGTGGAAGAAGAAGTTGAAGGAGAGCATGTGGAGTCGGCCGACTACATTTTCAAAAGGATCGGCGACCCCCTCCCAATCACGCCTCATGATTCCAGTTTCGATCCCCGAGGCAGCCCCTCCCGGCCCCTCGCCGTCTCCGAGAAACACGGCCTCGTCTTCGTCGCCCATTCCTCTGGGTTTTGCGTTGCGAGGACCAAAGATATTATGGCTTCGGCCGCCGAGATGGAGGAAAAGGGAAGCTATTCTTCTGTACAGGAACTGAGTATTGTGGATGTTCCCCTTCCCAACATACACATTCTCGCACTCTCTACCGACGGCTCTACTCTCGCAGCTACCGTAGATGCAGACATTCACTTCTTCTCTGTCGAATCACTTCTCCATAAGGGTCTCAAACCATCATTATCTTGTTCACTCAATGAATCCAACTCTATCAAGGATATGCAGTGGACAAGGAAACCTGAAAACCTTTATGTGGTCCTTTCGGATCTTGGGAAGTTATCTCATGGATCGGTTGGTGGCCTTATGGAAGATGTAATGGACAATGTTGATGCTGTTGGATGGAGTTCTAAAGGAAACTTAATTGTCGTGGCAAGAAAGGATAATATCAGCATTCTATCATCTAACTTTGAGGAGAGGTCATCTATGTTAATTTCGTTCAAACCCTGGGATGAAGATTCTGATGAAAACTGCATTGTAAAAGTGGATTCTATCAGGTGGGTACGCCATGATAGCATCATTCTAGGATGCTTTCAACTGACTGCTCAGGGCAATGAAAAAGGTTACCTTGTTCAAGTAATAAAAGTCAAGGATGGCAAATTTACTGATGATTCTTGCAAGCCAGTTGTGATATCTTTCTATGATTTGTTTTCAAATGTCATTGATGACATTCTGCCCTCTGCAAGTGGACCATACTTGTTATTGAGCTATCTGGAGCAATGTGAACTTGCAATTGTCGCTAACAGGAAGAATGTGGACGAGCATATTGTGTATCTCAGTTGGTCGCTAGGAGAGGAGAAGAATGAAGTTGTAGTTGTTGATATTTTCCAAGATAGTTTGCTACCAAGGATTGAGCTTCAAGATAATGGTGATGATAATTTGATTCTGGGGCTCTCAGTAGATAAAGTTTCAATAAGTAAAAAGATTAGTGTACGGTTTGGAGAGGAACACAAAGAACTCTCACCATTTTGTGTTCTGATGTGTCTTACGCTAGAGGGAAAACTGATTATGTTTCATGTTGCCAGTGTCAGCCGCATTACAGCCTCACCTGCTGTTGTTTCCTCCCTTTCTGATGGTGAGGAGGATTCCACTGCATTGGTACCTGTAGAAAGTGAATCAACTAAACCTTCTTTCCAGCTGGGGAAGGAACAATTTGAAAAGGCTTCTATGGATGTTCCATTGGTTAATGAGAGCAGAAAAGAACTTGACAGAAAAGTAGGTCTTGATGTCCATGTGAAAGATGACTTGAAATCTCTTAACGCGAATGAGACAGTGATGCCTGGACTTCTTACTCAAATACTCAATAAAGAAATTACTAGTTACAAGGAAGTGGAGCCCCTGAAAAACACTCAATCATTTAAAGCTGAAGGGAAACAAGAAGTTATTGTCCCAAAGCTACACGAAGACGTAGATGGAAACCAAATACAGCTTCCTGGACTGGGAAACAGAAATACTGAGCAAGCATCTACCAACGCTTCTCTTCAAGAAGGGCCTAGTTATGTGTTCAGGGACTTTAGCAAGACGGAGGCTAAAAATATTGAAGGACGAGGAACTGGCAGTGTCTCTTTTGGTGGTAACATTGCTGCTGATGCTGCTATAAAATCAAATTCAAATGATACACGTAATAATTTTGGGATGGGGACAGAATCTCCTGGAAAAATTGAGTCAGCGGGTTTCCAAAGTGTTTCATCTCAGTCACGGTTTGGTGGAAAAATCGTATCTTCAAAGGACACTGACGTGAAATCACCACTCATAACTTCAACTTCTATTCAAGGCAGCAGGTCTGGAAATGCTAGCCAAACTGTTGCTGCTTCAGGTGCTCATGGGAAACATTTTGGAAAACCTATTAATTTCAAGGATGTCTCTGGTAAACCTACTTCAGTCAACTCTTCTGATAGGCTTGGGGAGATTGGCAAATGGAGACCATCAGCGGCGACTGGAAATATTGTGTCAGTGCCTTCCATTTCAAGCTCCCAAACCTTATCACATGGAAGTTTTTCCTTTGCGAATTCTCCCAACCAGTCAAGGATGCCAAACTCCGAACCAAATTTATCAAAACAATTTGGCAATATCAAAGAGATGACCAAGGAGTTGGATATGCTTCTGCAATCTATAGAGGGAGCAGGTGGTTTCAGGGATGCCTGCACCGTTAATCAGAAAGGGTCAGTTGAAGAATTGGAGAGAGGCATAGAGACTCTGTCTGACAGATGCCGACTGTGGAAGAGCATTATGGATGAACGACTTCGAGAAATACAACATCTGCTTGACACAACCGTACAAGTATTGGCAAGGAAAATATACATGGAGGGCATTGTGAAGCAAGCTTCTGATAGACGATACTGGGACTTCTGGAATTGCCAGAAATTGAGTTCAGAACTTGAGCTTAAGCGGCGGCATATTTTAAAAATGAACCAGGATTTGACTGACCAATTAATTCAGTTAGAAAGGCATTTTAATGGCCTTGAACTCAATAAATTTGGTGAAAATGCTGGAGCTCGTGCTGGTGGAAGAGCCTTGCAGAGTCGATTTGGGCCCCCAAGACACATTCAGTCCTTGCATAGTTTACATAGCACAATGACGTCACAATTAGCCGCCGCTGATCAACTTTCTGAGTGCCTTTCAAAACAAATGGCAGCACTGAAGATAGAGTCACCTTCTGTAAAGAAGCAGAATGTAAAAAAAGAGTTGTTTGAAACAATAGGGATTCCCTATGATGCCTCCTTCAGTTCCCCTTCACGTACTCCAAATGAAAAACTATCATTTTCTTTTGGTTCTGCTGCTTCTAAAGATCAGCCCAGGAGAAATGTTAATGCCATAAAAAATTATGAACCAGAAACTGCAAGGAGGAGGAGAGACTCACTGGATAGGAGCTGGGAAAGTTATGAGCCTACAAAAGCAACTGTGAAAAGGTTGCTTTTGAAAGAGAGTGGCAAGGAAAGCATTAATAGATCATCATTTCCCATTGATAAGCAACAGTTAAGTCCTTGCCTGCTGGAGGGATCAGCAGTTACTCGCCCAAGGGACCACACAAGCCCTACATCCTTCTTGCATCCATCCGTAAACAAATGTATTCAGGGTACACAACCGAAACAGGCTTTTGAGAGCTCAGCAACGCCATTTGTTTGGGCTAGTGATCTTCCGGGACCATTCCAACCTACTGGGCTGAAGTCTCTTACGCAAGAACACAAAATGTTAGCAACATCCCAAGTATTCCCTGCAGCCAGGCAAAATTTTACAAGGGAAACTAGCATGACAGCTGAAAGATCTGGCGATGGCATGGCATATATTGGGAAGTATGATTCAGTTCCTATGAAGGAAAAGCCTGTCTTACCATCAGACACTACTCAGAAACCATCATTTTCTAAGGTATCTACACAGACACCATCTTTGCAGAAGAGACAGAATGATATGCTAAATGCATATGCTAAAGATACTGCACTTCCACCAAAAGAAAGTGTGAAGGATGGGCCTTTAACCACAAGAATGGCATCTACTGAAGCTGGGAAAAAACATGACTTTCCATTTTCTCCCTCATTTTCTGTTCCTGTGATTCCTAGCGAGCCTGGAAAGTTTGCCCAGACCGATGCTGCAACAAATAAAAGCCAGACTGTTAAAATGCCACCACCTACCACATTCTTAATGTCAGTTTCTGCACCCTCATCTCCAATAATTAGTTCATCATCGGCTCCTCTATCTTCGTCATCAATTCCGCAATCAGTTGCACCAACCTTTTCCTCAGCGATGCCCTTAAACAAATCATTGGCTAGTTCTGTCACTGCTGCAGATGAAAGCAAACCTGGTAAACCTGTATCAACATCCTCATCATCTTTTTTCTCCCCATTTGTTTCTTCTTCTAGTTCTCTCTCTTTTAACGTTTCAAATTCACTGGTGTCTTCATCTACCCCTTCACCTTTAGTGAGTTCAACATCAGAATCTCCAAAGACAGAGATCCAACCACCTTCAAAACCAGACATGAATGCTAATACTACATCACCACGCATGGAATTTGGGCCTTCCACGGTTGAAGGTAATTTGGAGCTTAAACCTTCAGTGTCATCTCCACCCTCAATTGAGACTTCAACAGGACTGTCAACTGGAGACGATCCGAGTCTTATCAAGGCTAGTCCTGCAGCAGTAGAGGCATCTGGGAGCCAGACCGGGATGAACGAAACTGCAGGCCCTAAACAGAATGTAACAGTCAATGATCAACAAGATCAGCCATCTGCTGGGCATTCTCCATTTCCAAATCTACCTACAACGTCAGGGAGTGTTACTGGTGGGATTGATGGCTTGGATTTACAAAATGCAGAGGAGGATGACATGGATGAGGAGACTCCGGACGCAAGTAGAGTAAGTGAACTTAGTTTGGGAAGCCTTGGTGGGTTTGGGCTTGGCTCTGGTCCTAAACCCAATCCATTTGGTGGATCATTTGGCAATGCACAAAATGCACAAACAAATGCGACTTCTCCTTTCTCCAGGCCTGTTGCTAGTGGAGAGTTATTTCAGCCTGCCTCCTTTAACTTCCAATCTCTACAGCCTTCCCAATCATCTCAGCCAGCAAATTCAGGTGCATTTGCTGGTGGCTTTGGCACCAGTACTACTGCCCAAGCTCCCATTCCGAGTGGGTTTGGCCAGCCAGCACAGGTTGGACCAGGGCAGCAAGCATTAGGGTCAGTTCTTGGTGCTTTTGGACAGTCAAGACAGATTGGTACTAGTCTACCAGGTACTGGTTTTGGATCACCCGGTGGTTTTGGTGGTGGCATTGCTGGCATTACTCCCACCGGTAGCTTCACAAGTGCTGCTACCGGAGGGTTTGCTGGTGCTGCTTCTGGTGGTGGTGGATTTGCTAGTTTGGCTTCAGGTGGCGGTGGTTCTGCTGTTGCGGCTTCAGGTGGTAGTGGTTTTGCTGCTGCAGCTTCGGGCGGTGGTGGATTTTCGGGTGCAGTTTCAGGCGGCGTGGGATTTGCTGGTGCAGCTTTCGGTGGAGGGGGCTTTGGTGGTGCAGCTTCAGGTGGTGGATTTGGGGCATTTGGTGGCCAACAAGGATCTGGCAGTTTCTCTGCTTTTGGTGGTGTAGGAGGAACGGGAAAGCCACCGGAGTTGTTCACACAGATGAGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05169 map03013 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1789

Amino Acids

189.46

Weight (kDa)

5.76

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_NUP159_NUP214 PF16755 49 - 255 2.4e-10 NUP159/214 beta propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1300, 2357
AatII GACGTC 1 cut(s) 2825
Acc36I ACCTGC 3 cut(s) 1300, 2013, 2357
Acc65I GGTACC 1 cut(s) 1342
AccB1I GGYRCC 2 cut(s) 1342, 4832
AccB7I CCANNNNNTGG 3 cut(s) 4469, 4664, 4970
AccI GTMKAC 1 cut(s) 4960
AccII CGCG 1 cut(s) 1505
AccIII TCCGGA 1 cut(s) 4579
AclI AACGTT 1 cut(s) 4116
AcoI YGGCCR 4 cut(s) 63, 240, 4873, 5278
AcsI RAATTY 9 cut(s) 842, 1849, 2251, 2290, 2717, 3472, 4123, 4247, 4804
AcyI GRCGYC 1 cut(s) 2822
AdeI CACNNNGTG 1 cut(s) 4988
AfiI CCNNNNNNNGG 6 cut(s) 499, 1682, 3238, 4469, 4664, 4970
AflII CTTAAG 1 cut(s) 2627
AflIII ACRYGT 1 cut(s) 1862
AgeI ACCGGT 1 cut(s) 5024
AhlI ACTAGT 2 cut(s) 1559, 4955
AjiI CACGTC 1 cut(s) 1981
AjuI GAANNNNNNNTTGG 6 cut(s) 1656, 1688, 3901, 3933, 4842, 4874
AleI CACNNNNGTG 1 cut(s) 1979
Alw21I GWGCWC 3 cut(s) 2065, 2740, 3350
AlwNI CAGNNNCTG 4 cut(s) 286, 3080, 3893, 4970
Ama87I CYCGRG 2 cut(s) 24, 132
Aor13HI TCCGGA 1 cut(s) 4579
ApaI GGGCCC 1 cut(s) 2778
ApoI RAATTY 9 cut(s) 842, 1849, 2251, 2290, 2717, 3472, 4123, 4247, 4804
ArsI GACNNNNNNTTYG 2 cut(s) 3613, 3645
AseI ATTAAT 3 cut(s) 2091, 2675, 3186
AsiGI ACCGGT 1 cut(s) 5024
Asp700I GAANNNNTTC 4 cut(s) 268, 399, 2239, 2484
Asp718I GGTACC 1 cut(s) 1342
AsuC2I CCSGG 4 cut(s) 148, 3384, 4402, 4986
AsuNHI GCTAGC 1 cut(s) 2036
AvaI CYCGRG 2 cut(s) 24, 132
AvaII GGWCC 7 cut(s) 219, 502, 932, 3265, 3386, 4643, 4892
BaeGI GKGCMC 1 cut(s) 2778
BalI TGGCCA 2 cut(s) 4875, 5280
BanI GGYRCC 2 cut(s) 1342, 4832
BanII GRGCYC 5 cut(s) 1137, 1581, 2740, 2778, 3350
BarI GAAGNNNNNNTAC 2 cut(s) 2104, 2136
BauI CACGAG 1 cut(s) 2739
BbsI GAAGAC 3 cut(s) 175, 1653, 4128
Bbv12I GWGCWC 3 cut(s) 2065, 2740, 3350
BbvCI CCTCAGC 1 cut(s) 3979
BceAI ACGGC 3 cut(s) 143, 190, 358
BclI TGATCA 2 cut(s) 2842, 4447
BcnI CCSGG 4 cut(s) 148, 3384, 4402, 4986
BcuI ACTAGT 2 cut(s) 1559, 4955
BfmI CTRYAG 8 cut(s) 1347, 2355, 3459, 4023, 4371, 4416, 4775, 5154
BfrI CTTAAG 1 cut(s) 2627
BfuAI ACCTGC 3 cut(s) 1300, 2013, 2357
BglII AGATCT 1 cut(s) 3506
BlpI GCTNAGC 1 cut(s) 3349
BmcAI AGTACT 1 cut(s) 4840
Bme18I GGWCC 7 cut(s) 219, 502, 932, 3265, 3386, 4643, 4892
BmeT110I CYCGRG 2 cut(s) 24, 132
BmgBI CACGTC 1 cut(s) 1981
BmrI ACTGGG 3 cut(s) 1691, 2593, 3411
BmtI GCTAGC 1 cut(s) 2040
BmuI ACTGGG 3 cut(s) 1691, 2593, 3411
BpiI GAAGAC 3 cut(s) 175, 1653, 4128
BpmI CTGGAG 5 cut(s) 977, 2754, 3257, 4359, 4702
Bpu10I CCTNAGC 2 cut(s) 789, 3979
Bpu1102I GCTNAGC 1 cut(s) 3349
BpuEI CTTGAG 1 cut(s) 2642
BpuMI CCSGG 4 cut(s) 148, 3384, 4402, 4986
BsaAI YACGTR 2 cut(s) 1865, 2981
BsaHI GRCGYC 1 cut(s) 2822
BsaI GGTCTC 2 cut(s) 419, 2158
BsaWI WCCGGW 4 cut(s) 4579, 5024, 5048, 5338
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 1876, 1906
Bsc4I CCNNNNNNNGG 6 cut(s) 499, 1682, 3238, 4469, 4664, 4970
Bse118I RCCGGY 1 cut(s) 5024
Bse3DI GCAATG 5 cut(s) 802, 968, 1821, 4684, 5004
BseAI TCCGGA 1 cut(s) 4579
BseLI CCNNNNNNNGG 6 cut(s) 499, 1682, 3238, 4469, 4664, 4970
BseMI GCAATG 5 cut(s) 802, 968, 1821, 4684, 5004
BseRI GAGGAG 8 cut(s) 671, 1052, 1337, 3103, 3106, 3921, 4568, 4586
BseSI GKGCMC 1 cut(s) 2778
BseX3I CGGCCG 2 cut(s) 63, 240
BseYI CCCAGC 4 cut(s) 1381, 3114, 3743, 4469
BsgI GTGCAG 6 cut(s) 2371, 3663, 3879, 5205, 5235, 5265
Bsh1236I CGCG 1 cut(s) 1505
Bsh1285I CGRYCG 2 cut(s) 66, 243
BshNI GGYRCC 2 cut(s) 1342, 4832
BshTI ACCGGT 1 cut(s) 5024
BsiEI CGRYCG 2 cut(s) 66, 243
BsiHKAI GWGCWC 3 cut(s) 2065, 2740, 3350
BsiHKCI CYCGRG 2 cut(s) 24, 132
BsiSI CCGG 8 cut(s) 148, 3383, 4401, 4580, 4986, 5025, 5049, 5339
BslFI GGGAC 6 cut(s) 1616, 1766, 1897, 2600, 3278, 3399
BslI CCNNNNNNNGG 6 cut(s) 499, 1682, 3238, 4469, 4664, 4970
BsmBI CGTCTC 2 cut(s) 166, 4335
BsmFI GGGAC 6 cut(s) 1616, 1766, 1897, 2600, 3278, 3399
BsmI GAATGC 3 cut(s) 636, 4225, 4474
Bso31I GGTCTC 2 cut(s) 419, 2158
BsoBI CYCGRG 2 cut(s) 24, 132
Bsp120I GGGCCC 1 cut(s) 2774
Bsp1286I GDGCHC 6 cut(s) 1137, 1581, 2065, 2740, 2778, 3350
Bsp13I TCCGGA 1 cut(s) 4579
Bsp1407I TGTACA 1 cut(s) 277
Bsp1720I GCTNAGC 1 cut(s) 3349
BspEI TCCGGA 1 cut(s) 4579
BspFNI CGCG 1 cut(s) 1505
BspHI TCATGA 1 cut(s) 112
BspMAI CTGCAG 5 cut(s) 3463, 4027, 4375, 4420, 5158
BspMI ACCTGC 3 cut(s) 1300, 2013, 2357
BspOI GCTAGC 1 cut(s) 2040
BspQI GCTCTTC 2 cut(s) 2459, 2746
BspT107I GGYRCC 2 cut(s) 1342, 4832
BspTI CTTAAG 1 cut(s) 2627
BspTNI GGTCTC 2 cut(s) 419, 2158
BsrDI GCAATG 5 cut(s) 802, 968, 1821, 4684, 5004
BsrFI RCCGGY 1 cut(s) 5024
BsrGI TGTACA 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 5024
BssNI GRCGYC 1 cut(s) 2822
BssSI CACGAG 1 cut(s) 2739
BssT1I CCWWGG 4 cut(s) 1088, 2328, 3260, 4618
Bst2BI CACGAG 1 cut(s) 2739
Bst6I CTCTTC 5 cut(s) 1728, 2137, 2459, 2746, 3632
BstACI GRCGYC 1 cut(s) 2822
BstAFI CTTAAG 1 cut(s) 2627
BstAPI GCANNNNNTGC 1 cut(s) 4688
BstAUI TGTACA 1 cut(s) 277
BstBAI YACGTR 2 cut(s) 1865, 2981
BstDSI CCRYGG 1 cut(s) 4260
BstEII GGTNACC 1 cut(s) 806
BstENI CCTNNNNNAGG 1 cut(s) 3236
BstFNI CGCG 1 cut(s) 1505
BstMCI CGRYCG 2 cut(s) 66, 243
BstNSI RCATGY 1 cut(s) 56
BstPI GGTNACC 1 cut(s) 806
BstSFI CTRYAG 8 cut(s) 1347, 2355, 3459, 4023, 4371, 4416, 4775, 5154
BstSLI GKGCMC 1 cut(s) 2778
BstUI CGCG 1 cut(s) 1505
BstV2I GAAGAC 3 cut(s) 175, 1653, 4128
BstX2I RGATCY 4 cut(s) 511, 3506, 4191, 5288
BstXI CCANNNNNNTGG 6 cut(s) 200, 1339, 2335, 3366, 3401, 3727
BstYI RGATCY 4 cut(s) 511, 3506, 4191, 5288
BstZI CGGCCG 2 cut(s) 63, 240
BtgI CCRYGG 1 cut(s) 4260
BtgZI GCGATG 2 cut(s) 3528, 3998
BtrI CACGTC 1 cut(s) 1981
BtsI GCAGTG 4 cut(s) 476, 1332, 1807, 4017
BveI ACCTGC 3 cut(s) 1300, 2013, 2357
CaiI CAGNNNCTG 4 cut(s) 286, 3080, 3893, 4970
CciI TCATGA 1 cut(s) 112
Cfr10I RCCGGY 1 cut(s) 5024
CseI GACGC 1 cut(s) 4592
CsiI ACCWGGT 2 cut(s) 4039, 4963
CspAI ACCGGT 1 cut(s) 5024
CspCI CAANNNNNGTGG 2 cut(s) 5325, 5360
DraI TTTAAA 2 cut(s) 1603, 2646
DraIII CACNNNGTG 1 cut(s) 4988
EaeI YGGCCR 4 cut(s) 63, 240, 4873, 5278
EagI CGGCCG 2 cut(s) 63, 240
Eam1104I CTCTTC 5 cut(s) 1728, 2137, 2459, 2746, 3632
EarI CTCTTC 5 cut(s) 1728, 2137, 2459, 2746, 3632
Ecl136II GAGCTC 2 cut(s) 2738, 3348
EclXI CGGCCG 2 cut(s) 63, 240
Eco130I CCWWGG 4 cut(s) 1088, 2328, 3260, 4618
Eco147I AGGCCT 1 cut(s) 4723
Eco24I GRGCYC 5 cut(s) 1137, 1581, 2740, 2778, 3350
Eco31I GGTCTC 2 cut(s) 419, 2158
Eco32I GATATC 1 cut(s) 876
Eco47I GGWCC 7 cut(s) 219, 502, 932, 3265, 3386, 4643, 4892
Eco52I CGGCCG 2 cut(s) 63, 240
Eco53kI GAGCTC 2 cut(s) 2738, 3348
Eco88I CYCGRG 2 cut(s) 24, 132
Eco91I GGTNACC 1 cut(s) 806
EcoICRI GAGCTC 2 cut(s) 2738, 3348
EcoNI CCTNNNNNAGG 1 cut(s) 3236
EcoO109I RGGNCCY 3 cut(s) 1733, 2775, 4421
EcoO65I GGTNACC 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 2251
EcoRV GATATC 1 cut(s) 876
EcoT14I CCWWGG 4 cut(s) 1088, 2328, 3260, 4618
EcoT22I ATGCAT 1 cut(s) 3664
EcoT38I GRGCYC 5 cut(s) 1137, 1581, 2740, 2778, 3350
ErhI CCWWGG 4 cut(s) 1088, 2328, 3260, 4618
Esp3I CGTCTC 2 cut(s) 166, 4335
FalI AAGNNNNNCTT 8 cut(s) 256, 288, 795, 827, 1449, 1481, 3554, 3586
FaqI GGGAC 6 cut(s) 1616, 1766, 1897, 2600, 3278, 3399
FauI CCCGC 1 cut(s) 1906
FauNDI CATATG 1 cut(s) 3664
FbaI TGATCA 2 cut(s) 2842, 4447
FblI GTMKAC 1 cut(s) 4960
FriOI GRGCYC 5 cut(s) 1137, 1581, 2740, 2778, 3350
GsaI CCCAGC 4 cut(s) 1385, 3118, 3747, 4473
GsuI CTGGAG 5 cut(s) 977, 2754, 3257, 4359, 4702
HapII CCGG 8 cut(s) 148, 3383, 4401, 4580, 4986, 5025, 5049, 5339
HgaI GACGC 1 cut(s) 4592
Hin1I GRCGYC 1 cut(s) 2822
HincII GTYRAC 2 cut(s) 2128, 4335
HindII GTYRAC 2 cut(s) 2128, 4335
HindIII AAGCTT 1 cut(s) 2565
HpaII CCGG 8 cut(s) 148, 3383, 4401, 4580, 4986, 5025, 5049, 5339
Hpy99I CGWCG 2 cut(s) 191, 344
HpyCH4IV ACGT 7 cut(s) 1650, 1864, 1980, 2822, 2980, 4116, 4502
HpySE526I ACGT 7 cut(s) 1650, 1864, 1980, 2822, 2980, 4116, 4502
Hsp92I GRCGYC 1 cut(s) 2822
Kpn2I TCCGGA 1 cut(s) 4579
KpnI GGTACC 1 cut(s) 1346
Ksp22I TGATCA 2 cut(s) 2842, 4447
LguI GCTCTTC 2 cut(s) 2459, 2746
MabI ACCWGGT 2 cut(s) 4039, 4963
MaeII ACGT 7 cut(s) 1650, 1864, 1980, 2822, 2980, 4116, 4502
MfeI CAATTG 2 cut(s) 975, 4311
MflI RGATCY 4 cut(s) 511, 3506, 4191, 5288
MhlI GDGCHC 6 cut(s) 1137, 1581, 2065, 2740, 2778, 3350
MlsI TGGCCA 2 cut(s) 4875, 5280
MluNI TGGCCA 2 cut(s) 4875, 5280
MlyI GAGTC 9 cut(s) 16, 68, 1916, 2431, 2773, 2900, 3092, 4360, 4570
MmeI TCCRAC 6 cut(s) 474, 562, 2316, 3418, 4219, 4870
Mox20I TGGCCA 2 cut(s) 4875, 5280
Mph1103I ATGCAT 1 cut(s) 3664
MroI TCCGGA 1 cut(s) 4579
MroXI GAANNNNTTC 4 cut(s) 268, 399, 2239, 2484
MscI TGGCCA 2 cut(s) 4875, 5280
MslI CAYNNNNRTG 4 cut(s) 1979, 2697, 3725, 5354
Msp20I TGGCCA 2 cut(s) 4875, 5280
MspA1I CMGCKG 5 cut(s) 1381, 1913, 2174, 2840, 3500
MspCI CTTAAG 1 cut(s) 2627
MspI CCGG 8 cut(s) 148, 3383, 4401, 4580, 4986, 5025, 5049, 5339
MunI CAATTG 2 cut(s) 975, 4311
Mva1269I GAATGC 3 cut(s) 636, 4225, 4474
MvnI CGCG 1 cut(s) 1505
NciI CCSGG 4 cut(s) 148, 3384, 4402, 4986
NdeI CATATG 1 cut(s) 3664
NheI GCTAGC 1 cut(s) 2036
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 5 cut(s) 19, 1938, 2823, 2892, 4015
NsiI ATGCAT 1 cut(s) 3664
NspI RCATGY 1 cut(s) 56
OliI CACNNNNGTG 1 cut(s) 1979
PaeR7I CTCGAG 1 cut(s) 24
PagI TCATGA 1 cut(s) 112
PaqCI CACCTGC 2 cut(s) 1300, 2357
PasI CCCWGGG 1 cut(s) 690
PceI AGGCCT 1 cut(s) 4723
PciSI GCTCTTC 2 cut(s) 2459, 2746
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 360
PctI GAATGC 3 cut(s) 636, 4225, 4474
PdmI GAANNNNTTC 4 cut(s) 268, 399, 2239, 2484
PflFI GACNNNGTC 1 cut(s) 2440
PflMI CCANNNNNTGG 3 cut(s) 4469, 4664, 4970
PfoI TCCNGGA 3 cut(s) 1675, 1894, 3382
PinAI ACCGGT 1 cut(s) 5024
PleI GAGTC 9 cut(s) 16, 67, 1915, 2431, 2772, 2899, 3092, 4359, 4570
PpsI GAGTC 9 cut(s) 16, 67, 1915, 2431, 2772, 2899, 3092, 4359, 4570
Ppu21I YACGTR 2 cut(s) 1865, 2981
PshBI ATTAAT 3 cut(s) 2091, 2675, 3186
Psp124BI GAGCTC 2 cut(s) 2740, 3350
Psp1406I AACGTT 1 cut(s) 4116
PspEI GGTNACC 1 cut(s) 806
PspFI CCCAGC 4 cut(s) 1381, 3114, 3743, 4469
PspOMI GGGCCC 1 cut(s) 2774
PspXI VCTCGAGB 1 cut(s) 24
PstI CTGCAG 5 cut(s) 3463, 4027, 4375, 4420, 5158
PstNI CAGNNNCTG 4 cut(s) 286, 3080, 3893, 4970
PsuI RGATCY 4 cut(s) 511, 3506, 4191, 5288
PsyI GACNNNGTC 1 cut(s) 2440
PvuII CAGCTG 2 cut(s) 1381, 3500
RseI CAYNNNNRTG 4 cut(s) 1979, 2697, 3725, 5354
SacI GAGCTC 2 cut(s) 2740, 3350
SapI GCTCTTC 2 cut(s) 2459, 2746
ScaI AGTACT 1 cut(s) 4840
SchI GAGTC 9 cut(s) 16, 68, 1916, 2431, 2773, 2900, 3092, 4360, 4570
SduI GDGCHC 6 cut(s) 1137, 1581, 2065, 2740, 2778, 3350
SexAI ACCWGGT 2 cut(s) 4039, 4963
SfcI CTRYAG 8 cut(s) 1347, 2355, 3459, 4023, 4371, 4416, 4775, 5154
Sfr274I CTCGAG 1 cut(s) 24
SinI GGWCC 7 cut(s) 219, 502, 932, 3265, 3386, 4643, 4892
SlaI CTCGAG 1 cut(s) 24
SmiMI CAYNNNNRTG 4 cut(s) 1979, 2697, 3725, 5354
SmlI CTYRAG 3 cut(s) 24, 2621, 2627
SmoI CTYRAG 3 cut(s) 24, 2621, 2627
SpeI ACTAGT 2 cut(s) 1559, 4955
SseBI AGGCCT 1 cut(s) 4723
SspI AATATT 2 cut(s) 1780, 2188
SstI GAGCTC 2 cut(s) 2740, 3350
StuI AGGCCT 1 cut(s) 4723
StyI CCWWGG 4 cut(s) 1088, 2328, 3260, 4618
TaiI ACGT 7 cut(s) 1653, 1867, 1983, 2825, 2983, 4119, 4505
TaqI TCGA 5 cut(s) 25, 126, 393, 2488, 2767
TaqII GACCGA 1 cut(s) 3839
TatI WGTACW 2 cut(s) 277, 4838
TauI GCSGC 9 cut(s) 245, 1278, 2177, 2635, 2638, 2837, 2840, 5129, 5199
TseFI GTSAC 5 cut(s) 19, 1938, 2823, 2892, 4015
Tsp45I GTSAC 5 cut(s) 19, 1938, 2823, 2892, 4015
TspGWI ACGGA 2 cut(s) 1781, 3289
Tth111I GACNNNGTC 1 cut(s) 2440
Van91I CCANNNNNTGG 3 cut(s) 4469, 4664, 4970
Vha464I CTTAAG 1 cut(s) 2627
VpaK11BI GGWCC 7 cut(s) 219, 502, 932, 3265, 3386, 4643, 4892
VspI ATTAAT 3 cut(s) 2091, 2675, 3186
XagI CCTNNNNNAGG 1 cut(s) 3236
XapI RAATTY 9 cut(s) 842, 1849, 2251, 2290, 2717, 3472, 4123, 4247, 4804
XceI RCATGY 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 2 cut(s) 229, 5288
XhoI CTCGAG 1 cut(s) 24
XmiI GTMKAC 1 cut(s) 4960
XmnI GAANNNNTTC 4 cut(s) 268, 399, 2239, 2484
ZraI GACGTC 1 cut(s) 2823
ZrmI AGTACT 1 cut(s) 4840
Zsp2I ATGCAT 1 cut(s) 3664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.