MD11G1082600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010549
Physical Location & Seq
Reverse (-)
7026250 .. 7027336
1087 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1082600.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGAGTGCTGTGGGAGGCCAAACAGGAGTGACGCTCATTTGTCGAGAGAGGAAGAGGCAGAGATCGAGTCGAAGACAAGAGAGTACTTCGATGGAGTGACTCCCAAGCATCACTCCAAACCTCAACGCAGTGAGTATTCATCAAAGTATGTGGACGATTTGAAAAACAAAGACGAAATTCCCGAACTTGTTGAGTTCCACCGTCTCGAAAGCGATTCTCAGAAAATAGTGCTAAACGGAAGTGAGGTTGGTGAGGAGTTTGTGGAGACAGACTACTACAAGGACCTGAACGAGACTGACAAGCAGCACCACACCACGGGAACTGGGTTCATCAAAATGCCCAACAAAAGCGACAACCGCTACAATTTGGCACCGGATTCCGACACCGACGTCCATGCCTCTTGCCAGTGCAACCCAGCAACTAATGACTGGATCCCAGATGATTCTGCTGCCAATACAGTATATTCAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.96

Weight (kDa)

4.75

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 1 cut(s) 393
AccB1I GGYRCC 1 cut(s) 370
AciI CCGC 1 cut(s) 358
AclWI GGATC 2 cut(s) 427, 440
AcsI RAATTY 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 2 cut(s) 163, 468
Alw26I GTCTC 3 cut(s) 209, 260, 287
AlwI GGATC 2 cut(s) 427, 440
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 2 cut(s) 304, 449
ApoI RAATTY 1 cut(s) 177
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 263
AvaII GGWCC 1 cut(s) 283
BamHI GGATCC 1 cut(s) 432
BanI GGYRCC 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 316, 436
BccI CCATC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BcoDI GTCTC 3 cut(s) 209, 260, 287
BisI GCNGC 2 cut(s) 305, 450
BlsI GCNGC 2 cut(s) 306, 451
BmcAI AGTACT 1 cut(s) 86
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 2 cut(s) 372, 434
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 118
BmuI ACTGGG 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 80
BplI GAGNNNNNCTC 2 cut(s) 19, 51
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 373
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 3 cut(s) 328, 406, 434
BseDI CCNNGG 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 3 cut(s) 328, 406, 434
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 2 cut(s) 316, 436
BseYI CCCAGC 1 cut(s) 415
BshFI GGCC 1 cut(s) 19
BshNI GGYRCC 1 cut(s) 370
BsiSI CCGG 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 3 cut(s) 209, 260, 287
BsmBI CGTCTC 1 cut(s) 209
BsnI GGCC 1 cut(s) 19
Bsp143I GATC 2 cut(s) 63, 432
BspACI CCGC 1 cut(s) 358
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 372, 434
BspPI GGATC 2 cut(s) 427, 440
BspT107I GGYRCC 1 cut(s) 370
BsrI ACTGG 3 cut(s) 328, 406, 434
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 2 cut(s) 63, 432
BssNI GRCGYC 1 cut(s) 390
Bst4CI ACNGT 2 cut(s) 203, 460
Bst6I CTCTTC 1 cut(s) 48
BstACI GRCGYC 1 cut(s) 390
BstDEI CTNAG 1 cut(s) 219
BstDSI CCRYGG 1 cut(s) 315
BstKTI GATC 2 cut(s) 66, 435
BstMAI GTCTC 3 cut(s) 209, 260, 287
BstMBI GATC 2 cut(s) 63, 432
BstMWI GCNNNNNNNGC 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 316, 436
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
BsuRI GGCC 1 cut(s) 19
BtgI CCRYGG 1 cut(s) 315
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 413
Cfr13I GGNCC 1 cut(s) 283
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 85
CspCI CAANNNNNGTGG 2 cut(s) 132, 167
CviAII CATG 1 cut(s) 395
CviJI RGCY 1 cut(s) 19
CviKI_1 RGCY 1 cut(s) 19
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 219
DpnI GATC 2 cut(s) 65, 434
DpnII GATC 2 cut(s) 63, 432
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 1 cut(s) 398
FaiI YATR 3 cut(s) 150, 396, 463
FatI CATG 1 cut(s) 394
Fnu4HI GCNGC 2 cut(s) 305, 450
Fsp4HI GCNGC 2 cut(s) 305, 450
GluI GCNGC 2 cut(s) 305, 450
GsaI CCCAGC 1 cut(s) 419
HaeIII GGCC 1 cut(s) 19
HapII CCGG 1 cut(s) 374
HgaI GACGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 1 cut(s) 398
HinfI GANTC 5 cut(s) 68, 100, 215, 377, 443
HpaII CCGG 1 cut(s) 374
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 222, 382
Hpy188III TCNNGA 3 cut(s) 45, 182, 206
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 2 cut(s) 203, 460
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 1 cut(s) 411
HpyF10VI GCNNNNNNNGC 1 cut(s) 357
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 1 cut(s) 398
Kzo9I GATC 2 cut(s) 63, 432
LpnPI CCDG 8 cut(s) 10, 299, 309, 387, 415, 419, 429, 450
Lsp1109I GCAGC 2 cut(s) 316, 436
LweI GCATC 1 cut(s) 118
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 29, 97
MalI GATC 2 cut(s) 65, 434
MboI GATC 2 cut(s) 63, 432
MboII GAAGA 2 cut(s) 65, 85
MflI RGATCY 1 cut(s) 432
MluCI AATT 3 cut(s) 177, 364, 468
MlyI GAGTC 2 cut(s) 77, 94
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 7 cut(s) 9, 43, 49, 132, 238, 247, 409
MseI TTAA 1 cut(s) 472
MslI CAYNNNNRTG 1 cut(s) 335
MspI CCGG 1 cut(s) 374
MwoI GCNNNNNNNGC 1 cut(s) 357
NdeII GATC 2 cut(s) 63, 432
NlaIII CATG 1 cut(s) 398
NlaIV GGNNCC 2 cut(s) 372, 434
NmuCI GTSAC 2 cut(s) 29, 97
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfeI GAWTC 3 cut(s) 215, 377, 443
PkrI GCNGC 2 cut(s) 306, 451
PleI GAGTC 2 cut(s) 76, 94
PpsI GAGTC 2 cut(s) 76, 94
PpuMI RGGWCCY 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 283
PspFI CCCAGC 1 cut(s) 415
PspN4I GGNNCC 2 cut(s) 372, 434
PspPI GGNCC 1 cut(s) 283
PspPPI RGGWCCY 1 cut(s) 283
PsuI RGATCY 1 cut(s) 432
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 335
SaqAI TTAA 1 cut(s) 472
SatI GCNGC 2 cut(s) 305, 450
Sau3AI GATC 2 cut(s) 63, 432
Sau96I GGNCC 1 cut(s) 283
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 2 cut(s) 77, 94
SetI ASST 4 cut(s) 124, 249, 288, 393
SfaNI GCATC 1 cut(s) 118
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 335
Sse9I AATT 3 cut(s) 177, 364, 468
SsiI CCGC 1 cut(s) 358
TaaI ACNGT 2 cut(s) 203, 460
TaiI ACGT 1 cut(s) 393
TaqI TCGA 5 cut(s) 44, 66, 71, 90, 207
TasI AATT 3 cut(s) 177, 364, 468
TatI WGTACW 1 cut(s) 84
TfiI GAWTC 3 cut(s) 215, 377, 443
Tru1I TTAA 1 cut(s) 472
Tru9I TTAA 1 cut(s) 472
TscAI CASTG 2 cut(s) 136, 413
TseFI GTSAC 2 cut(s) 29, 97
TseI GCWGC 2 cut(s) 304, 449
Tsp45I GTSAC 2 cut(s) 29, 97
TspDTI ATGAA 2 cut(s) 129, 319
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 2 cut(s) 136, 413
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 1 cut(s) 177
ZraI GACGTC 1 cut(s) 391
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.