Rorug05G0388200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010549
Physical Location & Seq
Reverse (-)
52725082 .. 52726507
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0388200.1

Sequence Viewer

Length: 594 bp
ATGGAGAGCTTGCTCCAACATCCATTCCCGAAAGTGCCCCAATTGAGACAATTTTTTGGCTCCGCCACTGCTTTTGCTGCACAGGGAATACTAACTAAGGTGACTTCTCTTGCTGCTCAAGAATTCAGTCTCGTATTGGGTTTCAAAGCTGATCTAGCACAGCTGCGTGACTCGTTCCTCAAGATTCAAGCTATGCTAAGAGACTTAGACCATTCACAAGTTCGGGGGGAGGTTGTGGAAATGTGGGTGAAGGATCTTGAAGACATAGCTCACGAAGGCGATGATGTGTTAGATGAAATTGGGTATGAATTTATCCGACGCAAAGTAGAAATGCAATACCAGATGAAGAAAAAGGTGAAAAGTTTCTTCTCCCACTCCAATCCCATTGCATTTCGCTCAAAAATGGCACATAAAATCAAGAAAATCAACACATCTTTGGTGAAATTATTCAATCAGGCAGTTGCCACTATTGGGTTAGTTGCTAGGACATTTGCTGATGCAAGTCATGCAATCTCACAAGATGTTGAAGTACTTGACAAGGAGACCTTCTCCAACTTTGATAGCGATGAGAAGCTCATCATTGGAAGGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

22.35

Weight (kDa)

6.6

Isoelectric Point (pI)

25.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 30 - 117 1.3e-19 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 63
AclWI GGATC 1 cut(s) 261
AcsI RAATTY 2 cut(s) 122, 308
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 1 cut(s) 471
AgsI TTSAA 5 cut(s) 145, 188, 260, 451, 527
AluBI AGCT 6 cut(s) 9, 149, 163, 191, 269, 574
AluI AGCT 6 cut(s) 9, 149, 163, 191, 269, 574
Alw26I GTCTC 4 cut(s) 40, 134, 195, 536
AlwI GGATC 1 cut(s) 261
ApeKI GCWGC 3 cut(s) 77, 113, 163
ApoI RAATTY 2 cut(s) 122, 308
Asp700I GAANNNNTTC 2 cut(s) 362, 446
AsuHPI GGTGA 4 cut(s) 112, 259, 367, 451
BaeGI GKGCMC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 267
BbvI GCAGC 3 cut(s) 64, 100, 150
BcoDI GTCTC 4 cut(s) 40, 134, 195, 536
BfaI CTAG 2 cut(s) 155, 483
BisI GCNGC 3 cut(s) 78, 114, 164
BlsI GCNGC 3 cut(s) 79, 115, 165
BmcAI AGTACT 1 cut(s) 531
BmiI GGNNCC 1 cut(s) 61
BmsI GCATC 1 cut(s) 487
BpiI GAAGAC 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 533, 565
BpuEI CTTGAG 2 cut(s) 102, 164
BsaI GGTCTC 1 cut(s) 536
Bsc4I CCNNNNNNNGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 384
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 1 cut(s) 471
BseMI GCAATG 1 cut(s) 384
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 3 cut(s) 64, 100, 150
BsgI GTGCAG 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 471
BsmAI GTCTC 4 cut(s) 40, 134, 195, 536
Bso31I GGTCTC 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 2 cut(s) 151, 253
BspACI CCGC 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 61
BspPI GGATC 1 cut(s) 261
BspTNI GGTCTC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 384
BssMI GATC 2 cut(s) 151, 253
BstAPI GCANNNNNTGC 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 11
BstDEI CTNAG 3 cut(s) 96, 197, 205
BstF5I GGATG 1 cut(s) 19
BstKTI GATC 2 cut(s) 154, 256
BstMAI GTCTC 4 cut(s) 40, 134, 195, 536
BstMBI GATC 2 cut(s) 151, 253
BstMWI GCNNNNNNNGC 3 cut(s) 77, 155, 506
BstSLI GKGCMC 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 64, 100, 150
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BtgZI GCGATG 2 cut(s) 294, 579
BtsCI GGATG 1 cut(s) 19
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 1 cut(s) 11
CseI GACGC 1 cut(s) 327
Csp6I GTAC 1 cut(s) 530
CspCI CAANNNNNGTGG 2 cut(s) 55, 90
CviAII CATG 1 cut(s) 506
CviJI RGCY 7 cut(s) 9, 60, 149, 163, 191, 269, 574
CviKI_1 RGCY 7 cut(s) 9, 60, 149, 163, 191, 269, 574
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 3 cut(s) 96, 197, 205
DpnI GATC 2 cut(s) 153, 255
DpnII GATC 2 cut(s) 151, 253
EciI GGCGGA 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 122
FaeI CATG 1 cut(s) 509
FaiI YATR 5 cut(s) 194, 266, 306, 411, 507
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FatI CATG 1 cut(s) 505
Fnu4HI GCNGC 3 cut(s) 78, 114, 164
FokI GGATG 1 cut(s) 6
Fsp4HI GCNGC 3 cut(s) 78, 114, 164
FspBI CTAG 2 cut(s) 155, 483
GluI GCNGC 3 cut(s) 78, 114, 164
HgaI GACGC 1 cut(s) 327
Hin1II CATG 1 cut(s) 509
HinfI GANTC 2 cut(s) 170, 184
HphI GGTGA 4 cut(s) 112, 259, 367, 451
Hpy188I TCNGA 1 cut(s) 317
Hpy188III TCNNGA 6 cut(s) 28, 119, 181, 257, 272, 418
Hpy99I CGWCG 1 cut(s) 321
HpyAV CCTTC 4 cut(s) 244, 269, 556, 579
HpyCH4V TGCA 5 cut(s) 80, 334, 389, 500, 509
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 155, 506
HpyF3I CTNAG 3 cut(s) 96, 197, 205
Hsp92II CATG 1 cut(s) 509
Kzo9I GATC 2 cut(s) 151, 253
LmnI GCTCC 2 cut(s) 18, 65
LpnPI CCDG 3 cut(s) 68, 353, 440
Lsp1109I GCAGC 3 cut(s) 64, 100, 150
LweI GCATC 1 cut(s) 487
MaeI CTAG 2 cut(s) 155, 483
MaeIII GTNAC 2 cut(s) 100, 167
MalI GATC 2 cut(s) 153, 255
MboI GATC 2 cut(s) 151, 253
MboII GAAGA 3 cut(s) 272, 358, 358
MfeI CAATTG 1 cut(s) 41
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 6 cut(s) 41, 50, 122, 297, 308, 443
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 3 cut(s) 40, 340, 576
MnlI CCTC 2 cut(s) 188, 223
MroXI GAANNNNTTC 2 cut(s) 362, 446
MspA1I CMGCKG 1 cut(s) 163
MunI CAATTG 1 cut(s) 41
MwoI GCNNNNNNNGC 3 cut(s) 77, 155, 506
NdeII GATC 2 cut(s) 151, 253
NlaIII CATG 1 cut(s) 509
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 2 cut(s) 100, 167
PdmI GAANNNNTTC 2 cut(s) 362, 446
PfeI GAWTC 1 cut(s) 184
PkrI GCNGC 3 cut(s) 79, 115, 165
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
PspN4I GGNNCC 1 cut(s) 61
PsuI RGATCY 1 cut(s) 253
PvuII CAGCTG 1 cut(s) 163
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
SatI GCNGC 3 cut(s) 78, 114, 164
Sau3AI GATC 2 cut(s) 151, 253
ScaI AGTACT 1 cut(s) 531
SchI GAGTC 1 cut(s) 164
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 1 cut(s) 487
SmlI CTYRAG 2 cut(s) 117, 179
SmoI CTYRAG 2 cut(s) 117, 179
Sse9I AATT 6 cut(s) 41, 50, 122, 297, 308, 443
SsiI CCGC 1 cut(s) 63
SspMI CTAG 2 cut(s) 155, 483
TasI AATT 6 cut(s) 41, 50, 122, 297, 308, 443
TatI WGTACW 1 cut(s) 529
TfiI GAWTC 1 cut(s) 184
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 2 cut(s) 100, 167
TseI GCWGC 3 cut(s) 77, 113, 163
Tsp45I GTSAC 2 cut(s) 100, 167
TspDTI ATGAA 3 cut(s) 309, 321, 359
TspRI CASTG 1 cut(s) 73
XapI RAATTY 2 cut(s) 122, 308
XmnI GAANNNNTTC 2 cut(s) 362, 446
XspI CTAG 2 cut(s) 155, 483
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.