Rh5DG478500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010549
Physical Location & Seq
Forward (+)
75528992 .. 75530149
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG478500.1

Sequence Viewer

Length: 504 bp
ATGGAGTGTTGTGGGAGACCTAACAGGAGTGACGTTCATTTGTCAAGTGAAGAAGAGGCAAAGATTGAGTCCGAGACAAGAGAACACTTTGATGGAGTAGCTCCCAAACGTCACTCGAAGCCTCAACGCAGCGAATACGCGTCTCAATATGTCGATGTTCTCTCTAAAGATGATAAACAAGAGTCCAGTCCTGAACTTGTTGAGTTCCAACGACTCGAAAATGATTCACAGAAATTGGTTCTGAATGGAAGTGAGGTGAGCGAGGAATTTGTGGAGACAGACTACTACAAAGATCTGAATTGCATTGACAAGCAACACCACACAACAGGAACAGGATTTATCAAAGTTCAAAACCCTGGCAACAGAGGTTACCGCCTTGAACCTGATTCCGACACCAGCACTCATGCGTCTTGCCAATGCAACCCAGCAACTAATGACTGGGTACCAGATGCTGCTAGTGAGGTACCTTCTGCTTCAGGCAAGCCAGGCAGAAGTGACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.69

Weight (kDa)

4.83

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 442, 463
AccB1I GGYRCC 2 cut(s) 442, 463
AccII CGCG 1 cut(s) 140
AciI CCGC 1 cut(s) 373
AcsI RAATTY 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 2 cut(s) 444, 465
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 2 cut(s) 350, 380
AjnI CCWGG 2 cut(s) 355, 484
AluBI AGCT 1 cut(s) 101
AluI AGCT 1 cut(s) 101
Alw26I GTCTC 4 cut(s) 10, 68, 147, 269
AlwNI CAGNNNCTG 1 cut(s) 452
ApeKI GCWGC 2 cut(s) 129, 452
ApoI RAATTY 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
Asp718I GGTACC 2 cut(s) 442, 463
AsuHPI GGTGA 1 cut(s) 268
BanI GGYRCC 2 cut(s) 442, 463
BbvI GCAGC 2 cut(s) 141, 439
BccI CCATC 1 cut(s) 86
BciT130I CCWGG 2 cut(s) 357, 486
BcoDI GTCTC 4 cut(s) 10, 68, 147, 269
BfaI CTAG 1 cut(s) 456
BglII AGATCT 1 cut(s) 292
BisI GCNGC 2 cut(s) 130, 453
BlsI GCNGC 2 cut(s) 131, 454
Bme1390I CCNGG 2 cut(s) 357, 486
BmiI GGNNCC 2 cut(s) 444, 465
BmrFI CCNGG 2 cut(s) 357, 486
BmrI ACTGGG 1 cut(s) 448
BmsI GCATC 1 cut(s) 439
BmuI ACTGGG 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 355
Bse1I ACTGG 2 cut(s) 186, 443
BseBI CCWGG 2 cut(s) 357, 486
BseDI CCNNGG 1 cut(s) 355
BseNI ACTGG 2 cut(s) 186, 443
BseXI GCAGC 2 cut(s) 141, 439
BseYI CCCAGC 1 cut(s) 424
Bsh1236I CGCG 1 cut(s) 140
BshNI GGYRCC 2 cut(s) 442, 463
BsmAI GTCTC 4 cut(s) 10, 68, 147, 269
BsmBI CGTCTC 1 cut(s) 147
Bso31I GGTCTC 1 cut(s) 10
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 1 cut(s) 373
BspFNI CGCG 1 cut(s) 140
BspLI GGNNCC 2 cut(s) 444, 465
BspT107I GGYRCC 2 cut(s) 442, 463
BspTNI GGTCTC 1 cut(s) 10
BsrI ACTGG 2 cut(s) 186, 443
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 357, 486
Bst6I CTCTTC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 482
BstEII GGTNACC 1 cut(s) 368
BstFNI CGCG 1 cut(s) 140
BstKTI GATC 1 cut(s) 295
BstMAI GTCTC 4 cut(s) 10, 68, 147, 269
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 1 cut(s) 486
BstNI CCWGG 2 cut(s) 357, 486
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 355, 484
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 141, 439
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 482
CaiI CAGNNNCTG 1 cut(s) 452
CseI GACGC 2 cut(s) 129, 396
Csp6I GTAC 2 cut(s) 443, 464
CviAII CATG 1 cut(s) 404
CviJI RGCY 3 cut(s) 101, 121, 484
CviKI_1 RGCY 3 cut(s) 101, 121, 484
CviQI GTAC 2 cut(s) 443, 464
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 459
Eco91I GGTNACC 1 cut(s) 368
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 355, 484
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 1 cut(s) 407
FaiI YATR 2 cut(s) 150, 405
FatI CATG 1 cut(s) 403
Fnu4HI GCNGC 2 cut(s) 130, 453
Fsp4HI GCNGC 2 cut(s) 130, 453
FspBI CTAG 1 cut(s) 456
GluI GCNGC 2 cut(s) 130, 453
GsaI CCCAGC 1 cut(s) 428
HgaI GACGC 2 cut(s) 129, 396
Hin1II CATG 1 cut(s) 407
HinfI GANTC 5 cut(s) 68, 182, 213, 224, 386
HphI GGTGA 1 cut(s) 268
Hpy188I TCNGA 4 cut(s) 73, 243, 297, 391
Hpy188III TCNNGA 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 477
HpyCH4IV ACGT 2 cut(s) 33, 109
HpyCH4V TGCA 2 cut(s) 303, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 486
HpySE526I ACGT 2 cut(s) 33, 109
Hsp92II CATG 1 cut(s) 407
KpnI GGTACC 2 cut(s) 446, 467
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 106
Lsp1109I GCAGC 2 cut(s) 141, 439
LweI GCATC 1 cut(s) 439
MaeI CTAG 1 cut(s) 456
MaeII ACGT 2 cut(s) 33, 109
MaeIII GTNAC 4 cut(s) 29, 110, 368, 494
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 2 cut(s) 62, 65
MflI RGATCY 1 cut(s) 292
MluCI AATT 3 cut(s) 233, 266, 298
MluI ACGCGT 1 cut(s) 138
MlyI GAGTC 3 cut(s) 77, 191, 207
MmeI TCCRAC 2 cut(s) 232, 414
MnlI CCTC 6 cut(s) 49, 132, 247, 256, 359, 454
MslI CAYNNNNRTG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 357, 486
MvaI CCWGG 2 cut(s) 357, 486
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 486
NdeII GATC 1 cut(s) 292
NlaIII CATG 1 cut(s) 407
NlaIV GGNNCC 2 cut(s) 444, 465
NmuCI GTSAC 3 cut(s) 29, 110, 494
PfeI GAWTC 2 cut(s) 224, 386
PkrI GCNGC 2 cut(s) 131, 454
PleI GAGTC 3 cut(s) 76, 190, 207
PpsI GAGTC 3 cut(s) 76, 190, 207
Psp6I CCWGG 2 cut(s) 355, 484
PspEI GGTNACC 1 cut(s) 368
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 355, 484
PspN4I GGNNCC 2 cut(s) 444, 465
PstNI CAGNNNCTG 1 cut(s) 452
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 2 cut(s) 444, 465
RsaNI GTAC 2 cut(s) 443, 464
RseI CAYNNNNRTG 1 cut(s) 90
SatI GCNGC 2 cut(s) 130, 453
Sau3AI GATC 1 cut(s) 292
SchI GAGTC 3 cut(s) 77, 191, 207
ScrFI CCNGG 2 cut(s) 357, 486
SetI ASST 9 cut(s) 22, 36, 103, 112, 258, 370, 385, 465, 469
SfaNI GCATC 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 3 cut(s) 233, 266, 298
SsiI CCGC 1 cut(s) 373
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 355, 484
TaiI ACGT 2 cut(s) 36, 112
TaqI TCGA 3 cut(s) 116, 153, 216
TasI AATT 3 cut(s) 233, 266, 298
TfiI GAWTC 2 cut(s) 224, 386
TseFI GTSAC 3 cut(s) 29, 110, 494
TseI GCWGC 2 cut(s) 129, 452
Tsp45I GTSAC 3 cut(s) 29, 110, 494
TspDTI ATGAA 1 cut(s) 26
XapI RAATTY 1 cut(s) 266
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.