Rroxscaffold_1G00012530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010549
Physical Location & Seq
Reverse (-)
15392952 .. 15394091
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00012530.1

Sequence Viewer

Length: 507 bp
ATGGAGTGTTGTGGGAGGCCTAACAGGAGTGACGTTCATTTGTCAAGTGAAGAAGAGGCAAAGATTGAGTCCGAGACAAGAGAACACTTTGATGGAGTAGCTCCCAAACGTCACTCGAAGCCTCAACGCAGCGAATACGCATCTCAATATGTCGATGTTCTCTCTAAAGATGGTAAACAAGAGTCCAGTCCTGAACTTGTTGAATTCCAACGACTCGAAAATGATTCACAGCAGAAATTGGTTCTGAATGGAAGTGAGGTGAGCGAGGAATTTGTGGAGACAGACTACTACAAGGATCTGAATTGCATTGACAAGCAACACCACACAACAGGAACAGGGTTTATCAAAGTTCAAAACCCTGGCAACAGAGGTTACCGCCTTGAACCTGATTCCGACACCGGCATTCATGCCTCTTGCCAGTGCAACCCAGCAACTAATGACTGGGTACCAGATGCTGCTAGTGCGGTACCTTCTGCTTCAGGCAAGCCAGGCAGAAGTGACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

18.69

Weight (kDa)

4.95

Isoelectric Point (pI)

52.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 445, 466
AccB1I GGYRCC 2 cut(s) 445, 466
AciI CCGC 2 cut(s) 376, 464
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 2 cut(s) 203, 269
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 2 cut(s) 447, 468
AgsI TTSAA 3 cut(s) 203, 353, 383
AjnI CCWGG 2 cut(s) 358, 487
AluBI AGCT 1 cut(s) 101
AluI AGCT 1 cut(s) 101
Alw26I GTCTC 2 cut(s) 68, 272
AlwI GGATC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 455
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 2 cut(s) 129, 455
ApoI RAATTY 2 cut(s) 203, 269
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
Asp718I GGTACC 2 cut(s) 445, 466
AsuHPI GGTGA 1 cut(s) 271
BanI GGYRCC 2 cut(s) 445, 466
BbvI GCAGC 2 cut(s) 141, 442
BccI CCATC 2 cut(s) 86, 164
BciT130I CCWGG 2 cut(s) 360, 489
BcoDI GTCTC 2 cut(s) 68, 272
BfaI CTAG 1 cut(s) 459
BisI GCNGC 2 cut(s) 130, 456
BlsI GCNGC 2 cut(s) 131, 457
Bme1390I CCNGG 2 cut(s) 360, 489
BmiI GGNNCC 2 cut(s) 447, 468
BmrFI CCNGG 2 cut(s) 360, 489
BmrI ACTGGG 1 cut(s) 451
BmsI GCATC 2 cut(s) 149, 442
BmuI ACTGGG 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 358
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 3 cut(s) 186, 418, 446
BseBI CCWGG 2 cut(s) 360, 489
BseDI CCNNGG 1 cut(s) 358
BseNI ACTGG 3 cut(s) 186, 418, 446
BseXI GCAGC 2 cut(s) 141, 442
BseYI CCCAGC 1 cut(s) 427
BshFI GGCC 1 cut(s) 19
BshNI GGYRCC 2 cut(s) 445, 466
BsiSI CCGG 1 cut(s) 399
BsmAI GTCTC 2 cut(s) 68, 272
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 1 cut(s) 19
Bsp143I GATC 1 cut(s) 295
BspACI CCGC 2 cut(s) 376, 464
BspANI GGCC 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 447, 468
BspPI GGATC 1 cut(s) 303
BspT107I GGYRCC 2 cut(s) 445, 466
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 3 cut(s) 186, 418, 446
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 1 cut(s) 295
Bst2UI CCWGG 2 cut(s) 360, 489
Bst6I CTCTTC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 485
BstEII GGTNACC 1 cut(s) 371
BstKTI GATC 1 cut(s) 298
BstMAI GTCTC 2 cut(s) 68, 272
BstMBI GATC 1 cut(s) 295
BstMWI GCNNNNNNNGC 2 cut(s) 461, 489
BstNI CCWGG 2 cut(s) 360, 489
BstPI GGTNACC 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 358, 487
BstV1I GCAGC 2 cut(s) 141, 442
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 1 cut(s) 485
CaiI CAGNNNCTG 1 cut(s) 455
Cfr10I RCCGGY 1 cut(s) 398
Csp6I GTAC 2 cut(s) 446, 467
CviAII CATG 1 cut(s) 407
CviJI RGCY 4 cut(s) 19, 101, 121, 487
CviKI_1 RGCY 4 cut(s) 19, 101, 121, 487
CviQI GTAC 2 cut(s) 446, 467
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Eco147I AGGCCT 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 462
Eco91I GGTNACC 1 cut(s) 371
EcoO65I GGTNACC 1 cut(s) 371
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 358, 487
FaeI CATG 1 cut(s) 410
FaiI YATR 2 cut(s) 150, 408
FatI CATG 1 cut(s) 406
Fnu4HI GCNGC 2 cut(s) 130, 456
Fsp4HI GCNGC 2 cut(s) 130, 456
FspBI CTAG 1 cut(s) 459
GluI GCNGC 2 cut(s) 130, 456
GsaI CCCAGC 1 cut(s) 431
HaeIII GGCC 1 cut(s) 19
HapII CCGG 1 cut(s) 399
Hin1II CATG 1 cut(s) 410
HinfI GANTC 5 cut(s) 68, 182, 213, 224, 389
HpaII CCGG 1 cut(s) 399
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 1 cut(s) 176
Hpy188I TCNGA 4 cut(s) 73, 246, 300, 394
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 1 cut(s) 176
HpyAV CCTTC 1 cut(s) 480
HpyCH4IV ACGT 2 cut(s) 33, 109
HpyCH4V TGCA 2 cut(s) 306, 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 489
HpySE526I ACGT 2 cut(s) 33, 109
Hsp92II CATG 1 cut(s) 410
KpnI GGTACC 2 cut(s) 449, 470
Kzo9I GATC 1 cut(s) 295
LmnI GCTCC 1 cut(s) 106
Lsp1109I GCAGC 2 cut(s) 141, 442
LweI GCATC 2 cut(s) 149, 442
MaeI CTAG 1 cut(s) 459
MaeII ACGT 2 cut(s) 33, 109
MaeIII GTNAC 4 cut(s) 29, 110, 371, 497
MalI GATC 1 cut(s) 297
MboI GATC 1 cut(s) 295
MboII GAAGA 2 cut(s) 62, 65
MflI RGATCY 1 cut(s) 295
MluCI AATT 4 cut(s) 203, 236, 269, 301
MlyI GAGTC 3 cut(s) 77, 191, 207
MmeI TCCRAC 2 cut(s) 232, 417
MnlI CCTC 7 cut(s) 9, 49, 132, 250, 259, 362, 421
MslI CAYNNNNRTG 1 cut(s) 90
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 2 cut(s) 360, 489
Mva1269I GAATGC 1 cut(s) 402
MvaI CCWGG 2 cut(s) 360, 489
MwoI GCNNNNNNNGC 2 cut(s) 461, 489
NdeII GATC 1 cut(s) 295
NlaIII CATG 1 cut(s) 410
NlaIV GGNNCC 2 cut(s) 447, 468
NmuCI GTSAC 3 cut(s) 29, 110, 497
PceI AGGCCT 1 cut(s) 19
PctI GAATGC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 224, 389
PkrI GCNGC 2 cut(s) 131, 457
PleI GAGTC 3 cut(s) 76, 190, 207
PpsI GAGTC 3 cut(s) 76, 190, 207
Psp6I CCWGG 2 cut(s) 358, 487
PspEI GGTNACC 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 427
PspGI CCWGG 2 cut(s) 358, 487
PspN4I GGNNCC 2 cut(s) 447, 468
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 2 cut(s) 447, 468
RsaNI GTAC 2 cut(s) 446, 467
RseI CAYNNNNRTG 1 cut(s) 90
SatI GCNGC 2 cut(s) 130, 456
Sau3AI GATC 1 cut(s) 295
SchI GAGTC 3 cut(s) 77, 191, 207
ScrFI CCNGG 2 cut(s) 360, 489
SetI ASST 7 cut(s) 36, 103, 112, 261, 373, 388, 472
SfaNI GCATC 2 cut(s) 149, 442
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 4 cut(s) 203, 236, 269, 301
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 2 cut(s) 376, 464
SspMI CTAG 1 cut(s) 459
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 2 cut(s) 358, 487
TaiI ACGT 2 cut(s) 36, 112
TaqI TCGA 3 cut(s) 116, 153, 216
TasI AATT 4 cut(s) 203, 236, 269, 301
TfiI GAWTC 2 cut(s) 224, 389
TscAI CASTG 1 cut(s) 425
TseFI GTSAC 3 cut(s) 29, 110, 497
TseI GCWGC 2 cut(s) 129, 455
Tsp45I GTSAC 3 cut(s) 29, 110, 497
TspDTI ATGAA 2 cut(s) 26, 395
TspRI CASTG 1 cut(s) 425
XapI RAATTY 2 cut(s) 203, 269
XspI CTAG 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.