MD11G1185000.v1.1

Phosphoesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011842
Physical Location & Seq
Forward (+)
24769415 .. 24776358
6944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1185000.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGCAGTCGCCGCCGCCATTTCCGTCCTTCTCCAACTGCTCGTAGGACTCTATAGTCCAGTCCATGCCAATCCCATAAAAAATATAGTTGTTCTAGTGATGGAGAACAGGTCGTTCGACCACATGCTAGGGTGGATGAAGAAAATCAACCCAGAAATCAACGGAGTGGACGGCTCCGAGTCCAACCCTCTCTCCACCACCGACCCCAACTCCAAGCGCTTCTTCTTCAAGAACGAGTCTCTCTATGTGGACCCTGATCCTGGTCACTCCTTCCAAGCCATCCGAGAGCAGATATTCGGGTCGGATAACACGGCGGCCGACCCGCCTCCCATGAATGGGTTTGCTCAGCAGGCTTACTCCATGGACAACACCACCGCCATGTCTCAGGACGTTATGAATGGATTCGATCCTGATATGGTTGCTGTCTACAAAACTCTTGTTTCTGAATTTGCCGTCTTTGACAGGTGGTTCGCCTCACTGCCAACCTCAACACAGCCGAATCGGCTCTACGTGCATTCAGGGACATCAGCTGGTGCAACAAGCAACATCCCAGCCCTTCTCGCCAAGGGTTACCCTCAAAGAACCATCTTCCAGAACCTCGATGACGCGGGAATATCCTTCGGGATATACTACCAAAACATTCCATCTACATTGTTCTACAGGAACTTGAGGAAGGTCAAGTACATATTCAAGTTCCATCGGTACAAAACCTTCAAGAGACATGCAAACGAAGGGAAACTACCCGGGTACACCGTCATAGAACAGAGGTACTTTGACATCAAGAGTGCACCTGCAAATGACGATCATCCCTCCCATGACGTGTACCACGGGCAAATGTTTGTAAAGGAGGTGTACGAGACACTAAGAGCAAGCCCTCAGTGGAACGACACCCTATTCATCATCACGTATGATGAACATGGGGGTTTCTATGATCATGTGCCTACACCGGTTCGTGGAGTCCCCAGCCCTGACAGGATCGTGGGGCCGGAACCTTTCTTCTTTCAGTTTGACCGGTTGGGAGTTAGGGTTCCAACGATCATGGTCTCACCATGGATAGAGAAGGGTACAGTCGTTCATGGTCCTAATGGATCGCCATTTCTGACTTCGGAATTTGAACACTCGTCTATTCCCGCAACAGTCAAGAAGATCTTCAACCTAACTGCACCCTTCCTAACAAAGAGGGATGAATGGGCTTGTACCTTTGAAGGCATTGTCCAAACACGGACAGAACCCCGAACTGACTGCCCAGAGCAACTGCCAACACCAGTCGCCATCAGAAAGAGTGAGCCTAACGAAGATGCCAAGCTTACAGAATTTCAGCAGGAGATGATACAACTCGCGGCAGTTCTAAAAGGAGACAACATATTTACAAGTTACCCCCAAAAGACAGGGAAGGCGATGACGGTCAAGGATGGGAAGAAATACGCAGATGATGCAGTTAAAGGATTCTTTGAAGGGGGGCTTTACGCTAAGAGAATGGGAGTTAATGAGGAGCGGATTGTCCAGATGAAACCTCCCTTACTTCGAGATCGTCCAGAACTTCACCAGAACACCCATAGAAATCTGGCAGTGGATCCTCGGGTGCATATGTAA

Protein Analysis

531

Amino Acids

59.88

Weight (kDa)

6.61

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosphoesterase PF04185 26 - 386 9.6e-107 Phosphoesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26870
fragaria_vesca FvH4_3g27850
malus_domestica MD03G1165700.v1.1 MD11G1185000.v1.1
prunus_persica Prupe.4G266000_v2.0.a1
pyrus_communis pycom03g12040 pycom12g08790
rosa_chinensis RchiOBHm_Chr5g0050581
rosa_laevigata RLG00000034707 RLG00000034708 RLG00000034709
rosa_multiflora Rmu_sc0001954.1_g000003
rosa_roxburghii Rroxscaffold_1G00030270
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG333700 Rh5BG343500 Rh5CG371100 Rh5DG356300
rosa_wichuraiana Rw5G031500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 799
AasI GACNNNNNNGTC 1 cut(s) 54
Acc36I ACCTGC 1 cut(s) 799
AccBSI CCGCTC 1 cut(s) 1495
AccI GTMKAC 1 cut(s) 426
AccII CGCG 2 cut(s) 608, 1340
AciI CCGC 9 cut(s) 12, 15, 314, 323, 375, 608, 1131, 1340, 1495
AclWI GGATC 6 cut(s) 251, 401, 983, 1096, 1568, 1581
AcoI YGGCCR 1 cut(s) 315
AcsI RAATTY 3 cut(s) 446, 1109, 1313
AfaI GTAC 8 cut(s) 683, 704, 749, 770, 824, 854, 1066, 1198
AfeI AGCGCT 1 cut(s) 218
AfiI CCNNNNNNNGG 4 cut(s) 260, 335, 336, 953
AflIII ACRYGT 1 cut(s) 819
AgeI ACCGGT 2 cut(s) 946, 1011
AgsI TTSAA 7 cut(s) 229, 691, 715, 1115, 1153, 1205, 1454
AjiI CACGTC 1 cut(s) 820
AjnI CCWGG 1 cut(s) 259
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AloI GAACNNNNNNTCC 2 cut(s) 1011, 1043
AluBI AGCT 2 cut(s) 530, 1306
AluI AGCT 2 cut(s) 530, 1306
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 6 cut(s) 243, 387, 712, 851, 1048, 1350
Alw44I GTGCAC 1 cut(s) 786
AlwI GGATC 6 cut(s) 251, 401, 983, 1096, 1568, 1581
Ama87I CYCGRG 2 cut(s) 743, 1578
Aor51HI AGCGCT 1 cut(s) 218
AoxI GGCC 2 cut(s) 315, 983
ApaLI GTGCAC 1 cut(s) 786
ApoI RAATTY 3 cut(s) 446, 1109, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
AsiGI ACCGGT 2 cut(s) 946, 1011
Asp700I GAANNNNTTC 2 cut(s) 401, 1148
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 3 cut(s) 250, 983, 1079
AsuC2I CCSGG 2 cut(s) 744, 745
AsuHPI GGTGA 2 cut(s) 1038, 1535
AvaI CYCGRG 2 cut(s) 743, 1578
AvaII GGWCC 2 cut(s) 250, 1079
BaeGI GKGCMC 1 cut(s) 790
BaeI ACNNNNGTAYC 2 cut(s) 1056, 1089
BamHI GGATCC 1 cut(s) 1573
BarI GAAGNNNNNNTAC 6 cut(s) 665, 695, 697, 723, 727, 755
Bbv12I GWGCWC 1 cut(s) 790
BccI CCATC 7 cut(s) 94, 287, 593, 652, 705, 1280, 1406
BceAI ACGGC 3 cut(s) 187, 327, 437
BcgI CGANNNNNNTGC 2 cut(s) 1224, 1258
BciT130I CCWGG 1 cut(s) 261
BclI TGATCA 1 cut(s) 931
BcnI CCSGG 2 cut(s) 744, 745
BcoDI GTCTC 6 cut(s) 243, 387, 712, 851, 1048, 1350
BfaI CTAG 2 cut(s) 95, 128
BfmI CTRYAG 2 cut(s) 52, 658
BfoI RGCGCY 1 cut(s) 220
BfuAI ACCTGC 1 cut(s) 799
BglI GCCNNNNNGGC 1 cut(s) 502
BglII AGATCT 1 cut(s) 1146
BisI GCNGC 4 cut(s) 12, 15, 315, 1341
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 4 cut(s) 13, 16, 316, 1342
Bme1390I CCNGG 3 cut(s) 261, 744, 745
Bme18I GGWCC 2 cut(s) 250, 1079
BmeT110I CYCGRG 2 cut(s) 743, 1578
BmgBI CACGTC 1 cut(s) 820
BmgT120I GGNCC 3 cut(s) 250, 983, 1079
BmiI GGNNCC 6 cut(s) 175, 252, 984, 990, 1029, 1575
BmrFI CCNGG 3 cut(s) 261, 744, 745
BmsI GCATC 2 cut(s) 1288, 1423
Bpu1102I GCTNAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 688
BpuMI CCSGG 2 cut(s) 744, 745
BsaAI YACGTR 2 cut(s) 511, 906
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 6 cut(s) 360, 564, 743, 826, 1049, 1577
BsaWI WCCGGW 2 cut(s) 946, 1011
BsaXI ACNNNNNCTCC 8 cut(s) 95, 125, 176, 194, 206, 224, 1011, 1041
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 335, 336, 953
Bse118I RCCGGY 2 cut(s) 946, 1011
Bse1I ACTGG 2 cut(s) 59, 1265
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 6 cut(s) 360, 564, 743, 826, 1049, 1577
BseGI GGATG 6 cut(s) 141, 279, 546, 805, 1189, 1417
BseLI CCNNNNNNNGG 4 cut(s) 260, 335, 336, 953
BseMII CTCAG 3 cut(s) 359, 398, 890
BseNI ACTGG 2 cut(s) 59, 1265
BseRI GAGGAG 1 cut(s) 1505
BseSI GKGCMC 1 cut(s) 790
BseX3I CGGCCG 1 cut(s) 315
BseYI CCCAGC 2 cut(s) 550, 962
BsgI GTGCAG 1 cut(s) 1146
Bsh1236I CGCG 2 cut(s) 608, 1340
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 2 cut(s) 317, 985
BshTI ACCGGT 2 cut(s) 946, 1011
BsiEI CGRYCG 1 cut(s) 318
BsiHKAI GWGCWC 1 cut(s) 790
BsiHKCI CYCGRG 2 cut(s) 743, 1578
BsiSI CCGG 4 cut(s) 744, 947, 986, 1012
BslFI GGGAC 2 cut(s) 535, 944
BslI CCNNNNNNNGG 4 cut(s) 260, 335, 336, 953
BsmAI GTCTC 6 cut(s) 243, 387, 712, 851, 1048, 1350
BsmFI GGGAC 2 cut(s) 535, 944
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 2 cut(s) 317, 985
Bso31I GGTCTC 1 cut(s) 1048
BsoBI CYCGRG 2 cut(s) 743, 1578
Bsp1286I GDGCHC 1 cut(s) 790
Bsp1720I GCTNAGC 1 cut(s) 345
Bsp19I CCATGG 2 cut(s) 360, 1049
BspACI CCGC 9 cut(s) 12, 15, 314, 323, 375, 608, 1131, 1340, 1495
BspANI GGCC 2 cut(s) 317, 985
BspCNI CTCAG 3 cut(s) 358, 397, 889
BspFNI CGCG 2 cut(s) 608, 1340
BspLI GGNNCC 6 cut(s) 175, 252, 984, 990, 1029, 1575
BspMI ACCTGC 1 cut(s) 799
BspPI GGATC 6 cut(s) 251, 401, 983, 1096, 1568, 1581
BspTNI GGTCTC 1 cut(s) 1048
BsrBI CCGCTC 1 cut(s) 1495
BsrFI RCCGGY 2 cut(s) 946, 1011
BsrI ACTGG 2 cut(s) 59, 1265
BssAI RCCGGY 2 cut(s) 946, 1011
BssECI CCNNGG 6 cut(s) 360, 564, 743, 826, 1049, 1577
BssT1I CCWWGG 3 cut(s) 360, 564, 1049
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 4 cut(s) 754, 1069, 1138, 1405
BstAPI GCANNNNNTGC 1 cut(s) 1433
BstBAI YACGTR 2 cut(s) 511, 906
BstC8I GCNNGC 2 cut(s) 351, 871
BstDEI CTNAG 5 cut(s) 345, 384, 863, 876, 1470
BstDSI CCRYGG 3 cut(s) 360, 826, 1049
BstEII GGTNACC 1 cut(s) 569
BstF5I GGATG 6 cut(s) 141, 279, 546, 805, 1189, 1417
BstFNI CGCG 2 cut(s) 608, 1340
BstH2I RGCGCY 1 cut(s) 220
BstHHI GCGC 1 cut(s) 219
BstMAI GTCTC 6 cut(s) 243, 387, 712, 851, 1048, 1350
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 6 cut(s) 11, 350, 502, 511, 560, 1433
BstNI CCWGG 1 cut(s) 261
BstNSI RCATGY 2 cut(s) 127, 725
BstPI GGTNACC 1 cut(s) 569
BstSCI CCNGG 3 cut(s) 259, 742, 743
BstSFI CTRYAG 2 cut(s) 52, 658
BstSLI GKGCMC 1 cut(s) 790
BstUI CGCG 2 cut(s) 608, 1340
BstX2I RGATCY 2 cut(s) 1146, 1573
BstYI RGATCY 2 cut(s) 1146, 1573
BstZI CGGCCG 1 cut(s) 315
BsuRI GGCC 2 cut(s) 317, 985
BtgI CCRYGG 3 cut(s) 360, 826, 1049
BtgZI GCGATG 1 cut(s) 1412
BtrI CACGTC 1 cut(s) 820
BtsCI GGATG 6 cut(s) 141, 279, 546, 805, 1189, 1417
BtsI GCAGTG 2 cut(s) 476, 1575
BtsIMutI CAGTG 3 cut(s) 476, 884, 1575
BveI ACCTGC 1 cut(s) 799
Cac8I GCNNGC 2 cut(s) 351, 871
CfoI GCGC 1 cut(s) 219
Cfr10I RCCGGY 2 cut(s) 946, 1011
Cfr13I GGNCC 3 cut(s) 250, 983, 1079
Cfr9I CCCGGG 1 cut(s) 743
CseI GACGC 1 cut(s) 614
Csp6I GTAC 8 cut(s) 682, 703, 748, 769, 823, 853, 1065, 1197
CspAI ACCGGT 2 cut(s) 946, 1011
CviQI GTAC 8 cut(s) 682, 703, 748, 769, 823, 853, 1065, 1197
DdeI CTNAG 5 cut(s) 345, 384, 863, 876, 1470
DrdI GACNNNNNNGTC 1 cut(s) 54
DseDI GACNNNNNNGTC 1 cut(s) 54
EaeI YGGCCR 1 cut(s) 315
EagI CGGCCG 1 cut(s) 315
EclXI CGGCCG 1 cut(s) 315
Eco130I CCWWGG 3 cut(s) 360, 564, 1049
Eco31I GGTCTC 1 cut(s) 1048
Eco47I GGWCC 2 cut(s) 250, 1079
Eco47III AGCGCT 1 cut(s) 218
Eco52I CGGCCG 1 cut(s) 315
Eco88I CYCGRG 2 cut(s) 743, 1578
Eco91I GGTNACC 1 cut(s) 569
EcoO65I GGTNACC 1 cut(s) 569
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 3 cut(s) 360, 564, 1049
ErhI CCWWGG 3 cut(s) 360, 564, 1049
FalI AAGNNNNNCTT 6 cut(s) 206, 238, 1133, 1165, 1446, 1478
FaqI GGGAC 2 cut(s) 535, 944
FauI CCCGC 3 cut(s) 330, 601, 1138
FauNDI CATATG 1 cut(s) 1587
FbaI TGATCA 1 cut(s) 931
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 4 cut(s) 12, 15, 315, 1341
FokI GGATG 6 cut(s) 148, 266, 533, 792, 1196, 1424
Fsp4HI GCNGC 4 cut(s) 12, 15, 315, 1341
FspBI CTAG 2 cut(s) 95, 128
GlaI GCGC 1 cut(s) 218
GluI GCNGC 4 cut(s) 12, 15, 315, 1341
GsaI CCCAGC 2 cut(s) 554, 966
HaeII RGCGCY 1 cut(s) 220
HaeIII GGCC 2 cut(s) 317, 985
HapII CCGG 4 cut(s) 744, 947, 986, 1012
HgaI GACGC 1 cut(s) 614
HhaI GCGC 1 cut(s) 219
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 1304
HinfI GANTC 7 cut(s) 48, 179, 236, 402, 499, 957, 1446
HpaII CCGG 4 cut(s) 744, 947, 986, 1012
HphI GGTGA 2 cut(s) 1038, 1535
Hpy166II GTNNAC 7 cut(s) 169, 250, 427, 750, 788, 823, 853
Hpy188I TCNGA 7 cut(s) 178, 284, 304, 445, 1101, 1108, 1277
Hpy8I GTNNAC 7 cut(s) 169, 250, 427, 750, 788, 823, 853
HpyCH4III ACNGT 4 cut(s) 754, 1069, 1138, 1405
HpyCH4IV ACGT 4 cut(s) 390, 510, 819, 905
HpyCH4V TGCA 8 cut(s) 514, 536, 725, 788, 794, 1163, 1436, 1585
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 350, 502, 511, 560, 1433
HpyF3I CTNAG 5 cut(s) 345, 384, 863, 876, 1470
HpySE526I ACGT 4 cut(s) 390, 510, 819, 905
HspAI GCGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 931
LmnI GCTCC 2 cut(s) 179, 1492
LweI GCATC 2 cut(s) 1288, 1423
MaeI CTAG 2 cut(s) 95, 128
MaeII ACGT 4 cut(s) 390, 510, 819, 905
MaeIII GTNAC 3 cut(s) 263, 569, 1373
MbiI CCGCTC 1 cut(s) 1495
MboII GAAGA 9 cut(s) 151, 214, 217, 580, 988, 1141, 1156, 1307, 1429
MflI RGATCY 2 cut(s) 1146, 1573
MhlI GDGCHC 1 cut(s) 790
MluCI AATT 3 cut(s) 446, 1109, 1313
MlyI GAGTC 4 cut(s) 42, 188, 245, 966
MmeI TCCRAC 4 cut(s) 58, 207, 282, 1055
MroXI GAANNNNTTC 2 cut(s) 401, 1148
MseI TTAA 2 cut(s) 1440, 1485
MslI CAYNNNNRTG 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 4 cut(s) 744, 947, 986, 1012
MspR9I CCNGG 3 cut(s) 261, 744, 745
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 1 cut(s) 261
MvnI CGCG 2 cut(s) 608, 1340
MwoI GCNNNNNNNGC 6 cut(s) 11, 350, 502, 511, 560, 1433
NciI CCSGG 2 cut(s) 744, 745
NcoI CCATGG 2 cut(s) 360, 1049
NdeI CATATG 1 cut(s) 1587
NlaIV GGNNCC 6 cut(s) 175, 252, 984, 990, 1029, 1575
NmuCI GTSAC 1 cut(s) 263
NspI RCATGY 2 cut(s) 127, 725
PaqCI CACCTGC 1 cut(s) 799
PctI GAATGC 1 cut(s) 514
PdmI GAANNNNTTC 2 cut(s) 401, 1148
PfeI GAWTC 3 cut(s) 402, 499, 1446
PinAI ACCGGT 2 cut(s) 946, 1011
PkrI GCNGC 4 cut(s) 13, 16, 316, 1342
PleI GAGTC 4 cut(s) 42, 187, 244, 965
PpsI GAGTC 4 cut(s) 42, 187, 244, 965
Ppu21I YACGTR 2 cut(s) 511, 906
Psp6I CCWGG 1 cut(s) 259
PspEI GGTNACC 1 cut(s) 569
PspFI CCCAGC 2 cut(s) 550, 962
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 6 cut(s) 175, 252, 984, 990, 1029, 1575
PspPI GGNCC 3 cut(s) 250, 983, 1079
PsuI RGATCY 2 cut(s) 1146, 1573
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 8 cut(s) 683, 704, 749, 770, 824, 854, 1066, 1198
RsaNI GTAC 8 cut(s) 682, 703, 748, 769, 823, 853, 1065, 1197
RseI CAYNNNNRTG 1 cut(s) 377
SaqAI TTAA 2 cut(s) 1440, 1485
SatI GCNGC 4 cut(s) 12, 15, 315, 1341
Sau96I GGNCC 3 cut(s) 250, 983, 1079
SchI GAGTC 4 cut(s) 42, 188, 245, 966
ScrFI CCNGG 3 cut(s) 261, 744, 745
SduI GDGCHC 1 cut(s) 790
SfaNI GCATC 2 cut(s) 1288, 1423
SfcI CTRYAG 2 cut(s) 52, 658
SinI GGWCC 2 cut(s) 250, 1079
SmaI CCCGGG 1 cut(s) 745
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 3 cut(s) 446, 1109, 1313
SsiI CCGC 9 cut(s) 12, 15, 314, 323, 375, 608, 1131, 1340, 1495
SspMI CTAG 2 cut(s) 95, 128
StyD4I CCNGG 3 cut(s) 259, 742, 743
StyI CCWWGG 3 cut(s) 360, 564, 1049
TaaI ACNGT 4 cut(s) 754, 1069, 1138, 1405
TaiI ACGT 4 cut(s) 393, 513, 822, 908
TaqI TCGA 4 cut(s) 117, 405, 600, 1525
TasI AATT 3 cut(s) 446, 1109, 1313
TatI WGTACW 1 cut(s) 681
TauI GCSGC 4 cut(s) 14, 17, 317, 1343
TfiI GAWTC 3 cut(s) 402, 499, 1446
Tru1I TTAA 2 cut(s) 1440, 1485
Tru9I TTAA 2 cut(s) 1440, 1485
TscAI CASTG 3 cut(s) 483, 884, 1575
TseFI GTSAC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 8 cut(s) 152, 347, 410, 886, 927, 1064, 1200, 1523
TspGWI ACGGA 3 cut(s) 13, 177, 1237
TspMI CCCGGG 1 cut(s) 743
TspRI CASTG 3 cut(s) 483, 884, 1575
VneI GTGCAC 1 cut(s) 786
VpaK11BI GGWCC 2 cut(s) 250, 1079
XapI RAATTY 3 cut(s) 446, 1109, 1313
XceI RCATGY 2 cut(s) 127, 725
XmaI CCCGGG 1 cut(s) 743
XmiI GTMKAC 1 cut(s) 426
XmnI GAANNNNTTC 2 cut(s) 401, 1148
XspI CTAG 2 cut(s) 95, 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.