MD11G1230300.v1.1

DAG protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016573
Physical Location & Seq
Reverse (-)
33463367 .. 33465501
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1230300.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCAATGGCGCCGAGCCTCTTTCTCTCATCCAAAGCCCTAATACTATCCCCAAGGCCCTCCCTTTTCCCTCCTTCTCCCCAACCCTCTTTCTCTACGCTCCGATTCAACTCGGATACTCGGCCTTTGACTCGCTCCGTTCCCTCTAAGCTGCGCGTTCGAGCTGTGAACGACAGTGACTACTCGTCGAGGAGAAGCAGCAGCAATGAGCAGCGGGAGACGATTATGTTACCGGGCTGTGACTATAACCACTGGCTTATAGTCATGGAGTTCCCTAAGGACCCTGCCCCAACTAGGGAGCAAATGATTGAGACCTACCTCAACACTCTCGCTTCTGTTCTTGGAAGCATGGAAGAAGCAAAGAAGAACATGTATGCTTTTAGCACCACCACCTACACTGGATTCCAATGTACTGTATCTGAAGAAACATCTGAAAAATTCAAAGGATTGCCTGGTGTTCTCTGGGTGTTGCCAGATTCATATATAGATGTTAAAAACAAGGATTATGGAGGTGATAAATATGTTAATGGAGAGATAATTCCTGGAAACTACCCCGTTTATCAACCAAAGAAACGGAGAGAATCAAAATTTGAGAGCAGAAGATATGAAAGACGAAGAGATGGACCTCCTCCTGAACGAAGAAAACCAAAACAAGAAGCAACTCCATCAGAATCAGCATCTGGATGA

Protein Analysis

229

Amino Acids

25.92

Weight (kDa)

9.33

Isoelectric Point (pI)

79.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 82 - 174 1e-41 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11430
fragaria_vesca FvH4_3g21470 FvH4_3g21470
malus_domestica MD03G1215700.v1.1 MD11G1230300.v1.1
prunus_persica Prupe.4G197100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0036911
rosa_laevigata RLG00000033733
rosa_roxburghii Rroxscaffold_1G00044050
rosa_rugosa Rorug05G0158700
rosa_samantha Rh5AG251200 Rh5BG252700 Rh5CG285200
rosa_wichuraiana Rw5G023090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 10
AccII CGCG 1 cut(s) 156
AciI CCGC 1 cut(s) 214
AcsI RAATTY 2 cut(s) 437, 587
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 2 cut(s) 294, 295
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 2 cut(s) 109, 442
AhdI GACNNNNNGTC 1 cut(s) 184
AjnI CCWGG 2 cut(s) 451, 541
AluBI AGCT 2 cut(s) 151, 164
AluI AGCT 2 cut(s) 151, 164
Alw26I GTCTC 2 cut(s) 212, 305
AlwNI CAGNNNCTG 1 cut(s) 680
AoxI GGCC 2 cut(s) 56, 122
ApeKI GCWGC 4 cut(s) 151, 198, 201, 211
ApoI RAATTY 2 cut(s) 437, 587
AspLEI GCGC 2 cut(s) 13, 156
AspS9I GGNCC 3 cut(s) 57, 280, 623
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 524
AvaII GGWCC 2 cut(s) 280, 623
AxyI CCTNAGG 1 cut(s) 276
BanI GGYRCC 1 cut(s) 10
BbvI GCAGC 4 cut(s) 138, 210, 213, 223
BccI CCATC 2 cut(s) 614, 673
BciT130I CCWGG 2 cut(s) 453, 543
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 212, 305
BfaI CTAG 1 cut(s) 294
BfoI RGCGCY 1 cut(s) 14
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 4 cut(s) 152, 199, 202, 212
BlsI GCNGC 4 cut(s) 153, 200, 203, 213
Bme1390I CCNGG 3 cut(s) 234, 453, 543
Bme18I GGWCC 2 cut(s) 280, 623
BmeRI GACNNNNNGTC 1 cut(s) 184
BmgT120I GGNCC 3 cut(s) 57, 280, 623
BmiI GGNNCC 2 cut(s) 12, 282
BmrFI CCNGG 3 cut(s) 234, 453, 543
BpuMI CCSGG 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 53
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 295
Bse1I ACTGG 2 cut(s) 257, 403
Bse21I CCTNAGG 1 cut(s) 276
Bse3DI GCAATG 2 cut(s) 12, 211
BseBI CCWGG 2 cut(s) 453, 543
BseDI CCNNGG 1 cut(s) 53
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 2 cut(s) 294, 295
BseMI GCAATG 2 cut(s) 12, 211
BseNI ACTGG 2 cut(s) 257, 403
BseRI GAGGAG 2 cut(s) 205, 618
BseXI GCAGC 4 cut(s) 138, 210, 213, 223
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 2 cut(s) 58, 124
BshNI GGYRCC 1 cut(s) 10
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 294, 295
BsmAI GTCTC 2 cut(s) 212, 305
BsmBI CGTCTC 1 cut(s) 212
BsnI GGCC 2 cut(s) 58, 124
Bso31I GGTCTC 1 cut(s) 305
BspACI CCGC 1 cut(s) 214
BspANI GGCC 2 cut(s) 58, 124
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 12, 282
BspT107I GGYRCC 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 12, 211
BsrI ACTGG 2 cut(s) 257, 403
BssECI CCNNGG 1 cut(s) 53
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 453, 543
Bst4CI ACNGT 2 cut(s) 176, 415
Bst6I CTCTTC 1 cut(s) 610
BstACI GRCGYC 1 cut(s) 11
BstDEI CTNAG 2 cut(s) 147, 276
BstF5I GGATG 1 cut(s) 29
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 2 cut(s) 13, 156
BstMAI GTCTC 2 cut(s) 212, 305
BstNI CCWGG 2 cut(s) 453, 543
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 3 cut(s) 232, 451, 541
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 4 cut(s) 138, 210, 213, 223
Bsu36I CCTNAGG 1 cut(s) 276
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 2 cut(s) 58, 124
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 3 cut(s) 181, 250, 396
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 2 cut(s) 13, 156
Cfr13I GGNCC 3 cut(s) 57, 280, 623
Csp6I GTAC 1 cut(s) 411
CviAII CATG 3 cut(s) 265, 349, 370
CviJI RGCY 8 cut(s) 18, 38, 58, 124, 151, 164, 237, 256
CviKI_1 RGCY 8 cut(s) 18, 38, 58, 124, 151, 164, 237, 256
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 2 cut(s) 147, 276
DinI GGCGCC 1 cut(s) 12
DriI GACNNNNNGTC 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 610
Eam1105I GACNNNNNGTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 610
Eco130I CCWWGG 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 2 cut(s) 280, 623
Eco57I CTGAAG 1 cut(s) 441
Eco81I CCTNAGG 1 cut(s) 276
EcoO109I RGGNCCY 2 cut(s) 57, 280
EcoRII CCWGG 2 cut(s) 451, 541
EcoT14I CCWWGG 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 12
EheI GGCGCC 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 53
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 3 cut(s) 268, 352, 373
FatI CATG 3 cut(s) 264, 348, 369
FauI CCCGC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 152, 199, 202, 212
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 4 cut(s) 152, 199, 202, 212
FspBI CTAG 1 cut(s) 294
GlaI GCGC 2 cut(s) 12, 155
GluI GCNGC 4 cut(s) 152, 199, 202, 212
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 2 cut(s) 58, 124
HapII CCGG 1 cut(s) 233
HhaI GCGC 2 cut(s) 13, 156
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 3 cut(s) 268, 352, 373
Hin6I GCGC 2 cut(s) 11, 154
HinP1I GCGC 2 cut(s) 11, 154
HinfI GANTC 6 cut(s) 105, 130, 402, 476, 581, 671
HpaII CCGG 1 cut(s) 233
HphI GGTGA 1 cut(s) 524
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 5 cut(s) 104, 115, 421, 433, 670
Hpy188III TCNNGA 2 cut(s) 632, 681
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 1 cut(s) 190
HpyAV CCTTC 1 cut(s) 84
HpyCH4III ACNGT 2 cut(s) 176, 415
HpyF3I CTNAG 2 cut(s) 147, 276
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 3 cut(s) 268, 352, 373
HspAI GCGC 2 cut(s) 11, 154
KasI GGCGCC 1 cut(s) 10
LmnI GCTCC 3 cut(s) 105, 140, 298
Lsp1109I GCAGC 4 cut(s) 138, 210, 213, 223
MaeI CTAG 1 cut(s) 294
MaeIII GTNAC 3 cut(s) 176, 228, 239
MboII GAAGA 6 cut(s) 365, 376, 434, 612, 627, 651
MluCI AATT 3 cut(s) 437, 537, 587
Mly113I GGCGCC 1 cut(s) 11
MlyI GAGTC 1 cut(s) 124
MseI TTAA 2 cut(s) 492, 525
MslI CAYNNNNRTG 1 cut(s) 682
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 234, 453, 543
MvaI CCWGG 2 cut(s) 453, 543
MvnI CGCG 1 cut(s) 156
NarI GGCGCC 1 cut(s) 11
NciI CCSGG 1 cut(s) 234
NlaIII CATG 3 cut(s) 268, 352, 373
NlaIV GGNNCC 2 cut(s) 12, 282
NmeAIII GCCGAG 2 cut(s) 39, 100
NmuCI GTSAC 2 cut(s) 176, 239
NspI RCATGY 1 cut(s) 373
PciI ACATGT 1 cut(s) 369
PfeI GAWTC 5 cut(s) 105, 402, 476, 581, 671
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 4 cut(s) 153, 200, 203, 213
PleI GAGTC 1 cut(s) 124
PluTI GGCGCC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 124
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 451, 541
PspGI CCWGG 2 cut(s) 451, 541
PspN4I GGNNCC 2 cut(s) 12, 282
PspPI GGNCC 3 cut(s) 57, 280, 623
PspPPI RGGWCCY 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 680
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 1 cut(s) 682
SaqAI TTAA 2 cut(s) 492, 525
SatI GCNGC 4 cut(s) 152, 199, 202, 212
Sau96I GGNCC 3 cut(s) 57, 280, 623
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 3 cut(s) 234, 453, 543
SetI ASST 7 cut(s) 153, 166, 317, 321, 395, 514, 628
SfoI GGCGCC 1 cut(s) 12
SinI GGWCC 2 cut(s) 280, 623
SmiMI CAYNNNNRTG 1 cut(s) 682
Sse9I AATT 3 cut(s) 437, 537, 587
SsiI CCGC 1 cut(s) 214
SspDI GGCGCC 1 cut(s) 10
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 3 cut(s) 232, 451, 541
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 2 cut(s) 176, 415
TaqI TCGA 2 cut(s) 160, 188
TasI AATT 3 cut(s) 437, 537, 587
TatI WGTACW 1 cut(s) 410
TfiI GAWTC 5 cut(s) 105, 402, 476, 581, 671
Tru1I TTAA 2 cut(s) 492, 525
Tru9I TTAA 2 cut(s) 492, 525
TscAI CASTG 3 cut(s) 181, 257, 403
TseFI GTSAC 2 cut(s) 176, 239
TseI GCWGC 4 cut(s) 151, 198, 201, 211
Tsp45I GTSAC 2 cut(s) 176, 239
TspDTI ATGAA 2 cut(s) 468, 621
TspGWI ACGGA 2 cut(s) 127, 589
TspRI CASTG 3 cut(s) 181, 257, 403
VpaK11BI GGWCC 2 cut(s) 280, 623
XapI RAATTY 2 cut(s) 437, 587
XceI RCATGY 1 cut(s) 373
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.