RLG00000033733

DAG protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016573
Physical Location & Seq
Forward (+)
31555688 .. 31557435
1748 bp
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UTR
Exon/CDS
Intron
RLM00000033733

Sequence Viewer

Length: 687 bp
ATGGCAATGGCGCCGAGCCTCTCTCTCACACCCAAAACCCTAATAATACCGACCCCAAGACTCTCCCTCTTCCAGCCCTCTATCTCCTCCCTCGGATTCAGCTCCTCCGCCGCCGCGACTCGCCCTCTTACTTGCTCGGCGCCGTCAAAGCCAACCCTTCGGGCTGTGAGCGACAGCGAGTACTCGTCCAGAAGGAGCAGCAGTAATGAGCAGAGGGAGACGATAATGTTACCGGGCTGTGACTATAACCACTGGCTCATAGTCATGGAGTTCCCCAAAGACCCTGCCCCTACTAGGGACCAAATGATTGAGACCTACCTCAACACTCTCGCTACTGTTCTCGGAAGTATGGAAGAAGCAAAGAAGAATATGTATGCTTTTAGTACCACCACCTACACTGGATTTCAGTGTACTGTATCCGAAGAAACATCTGAAAAATTTAAAGGCTTGCCTGGTGTCCTCTGGGTGTTGCCAGATTCGTATATAGATGTTAAAAATAAGGATTATGGAGGTGATAAATATGTTAATGGAGAGATAATTCCTGGTAACTACCCTGTTTATCAACCGAAGAAACGAAGAGAATCAAAGTTTGAGAGCAGAAGATATGAAAGACGAAGAGATGGACCTCCTCCAGAACGAACACGGCCAAAGCAAGAAGCAACTCCATCAGAATCAGCATCTGGTTGA

Protein Analysis

229

Amino Acids

25.5

Weight (kDa)

8.87

Isoelectric Point (pI)

67.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 82 - 174 1.1e-41 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11430
fragaria_vesca FvH4_3g21470 FvH4_3g21470
malus_domestica MD03G1215700.v1.1 MD11G1230300.v1.1
prunus_persica Prupe.4G197100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0036911
rosa_laevigata RLG00000033733
rosa_roxburghii Rroxscaffold_1G00044050
rosa_rugosa Rorug05G0158700
rosa_samantha Rh5AG251200 Rh5BG252700 Rh5CG285200
rosa_wichuraiana Rw5G023090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 10, 139
AccII CGCG 1 cut(s) 116
AciI CCGC 3 cut(s) 108, 111, 114
AcoI YGGCCR 1 cut(s) 644
AcsI RAATTY 1 cut(s) 437
AcyI GRCGYC 2 cut(s) 11, 140
AfaI GTAC 3 cut(s) 182, 385, 412
AfiI CCNNNNNNNGG 2 cut(s) 294, 295
AjnI CCWGG 2 cut(s) 451, 541
AluBI AGCT 1 cut(s) 102
AluI AGCT 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 212, 305
AlwNI CAGNNNCTG 1 cut(s) 680
AoxI GGCC 1 cut(s) 644
ApeKI GCWGC 1 cut(s) 198
ApoI RAATTY 1 cut(s) 437
AspLEI GCGC 2 cut(s) 13, 142
AspS9I GGNCC 2 cut(s) 298, 623
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 524
AvaII GGWCC 2 cut(s) 298, 623
BanI GGYRCC 2 cut(s) 10, 139
BbvI GCAGC 1 cut(s) 210
BccI CCATC 2 cut(s) 614, 673
BceAI ACGGC 2 cut(s) 127, 659
BciT130I CCWGG 2 cut(s) 453, 543
BciVI GTATCC 1 cut(s) 427
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 212, 305
BfaI CTAG 1 cut(s) 294
BfoI RGCGCY 2 cut(s) 14, 143
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 3 cut(s) 111, 114, 199
BlsI GCNGC 3 cut(s) 112, 115, 200
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 3 cut(s) 234, 453, 543
Bme18I GGWCC 2 cut(s) 298, 623
BmgT120I GGNCC 2 cut(s) 298, 623
BmiI GGNNCC 3 cut(s) 12, 141, 299
BmrFI CCNGG 3 cut(s) 234, 453, 543
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 615
BpuMI CCSGG 1 cut(s) 234
BsaHI GRCGYC 2 cut(s) 11, 140
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 295
Bse1I ACTGG 2 cut(s) 257, 403
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 2 cut(s) 453, 543
BseDI CCNNGG 1 cut(s) 91
BseLI CCNNNNNNNGG 2 cut(s) 294, 295
BseMI GCAATG 1 cut(s) 12
BseNI ACTGG 2 cut(s) 257, 403
BseRI GAGGAG 3 cut(s) 76, 94, 618
BseXI GCAGC 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 1 cut(s) 646
BshNI GGYRCC 2 cut(s) 10, 139
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 294, 295
BsmAI GTCTC 2 cut(s) 212, 305
BsmBI CGTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 1 cut(s) 646
Bso31I GGTCTC 1 cut(s) 305
BspACI CCGC 3 cut(s) 108, 111, 114
BspANI GGCC 1 cut(s) 646
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 3 cut(s) 12, 141, 299
BspT107I GGYRCC 2 cut(s) 10, 139
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 2 cut(s) 257, 403
BssECI CCNNGG 1 cut(s) 91
BssNI GRCGYC 2 cut(s) 11, 140
Bst2UI CCWGG 2 cut(s) 453, 543
Bst4CI ACNGT 2 cut(s) 337, 415
Bst6I CTCTTC 3 cut(s) 74, 571, 610
BstACI GRCGYC 2 cut(s) 11, 140
BstC8I GCNNGC 1 cut(s) 449
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 2 cut(s) 14, 143
BstHHI GCGC 2 cut(s) 13, 142
BstMAI GTCTC 2 cut(s) 212, 305
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNI CCWGG 2 cut(s) 453, 543
BstSCI CCNGG 3 cut(s) 232, 451, 541
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 210
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 1 cut(s) 646
BtsIMutI CAGTG 3 cut(s) 250, 396, 413
Cac8I GCNNGC 1 cut(s) 449
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 2 cut(s) 13, 142
Cfr13I GGNCC 2 cut(s) 298, 623
Csp6I GTAC 3 cut(s) 181, 384, 411
CviAII CATG 1 cut(s) 265
CviJI RGCY 9 cut(s) 18, 76, 102, 151, 164, 237, 256, 447, 646
CviKI_1 RGCY 9 cut(s) 18, 76, 102, 151, 164, 237, 256, 447, 646
CviQI GTAC 3 cut(s) 181, 384, 411
DinI GGCGCC 2 cut(s) 12, 141
DraI TTTAAA 1 cut(s) 442
EaeI YGGCCR 1 cut(s) 644
Eam1104I CTCTTC 3 cut(s) 74, 571, 610
EarI CTCTTC 3 cut(s) 74, 571, 610
EciI GGCGGA 1 cut(s) 97
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 2 cut(s) 298, 623
EcoRII CCWGG 2 cut(s) 451, 541
EgeI GGCGCC 2 cut(s) 12, 141
EheI GGCGCC 2 cut(s) 12, 141
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 1 cut(s) 268
FaqI GGGAC 1 cut(s) 311
FatI CATG 1 cut(s) 264
Fnu4HI GCNGC 3 cut(s) 111, 114, 199
Fsp4HI GCNGC 3 cut(s) 111, 114, 199
FspBI CTAG 1 cut(s) 294
GlaI GCGC 2 cut(s) 12, 141
GluI GCNGC 3 cut(s) 111, 114, 199
GsuI CTGGAG 1 cut(s) 615
HaeII RGCGCY 2 cut(s) 14, 143
HaeIII GGCC 1 cut(s) 646
HapII CCGG 1 cut(s) 233
HhaI GCGC 2 cut(s) 13, 142
Hin1I GRCGYC 2 cut(s) 11, 140
Hin1II CATG 1 cut(s) 268
Hin6I GCGC 2 cut(s) 11, 140
HinP1I GCGC 2 cut(s) 11, 140
HinfI GANTC 6 cut(s) 60, 96, 118, 476, 581, 671
HpaII CCGG 1 cut(s) 233
HphI GGTGA 1 cut(s) 524
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 5 cut(s) 95, 344, 421, 433, 670
Hpy188III TCNNGA 2 cut(s) 189, 632
Hpy8I GTNNAC 1 cut(s) 411
HpyAV CCTTC 2 cut(s) 167, 186
HpyCH4III ACNGT 2 cut(s) 337, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
Hsp92I GRCGYC 2 cut(s) 11, 140
Hsp92II CATG 1 cut(s) 268
HspAI GCGC 2 cut(s) 11, 140
KasI GGCGCC 2 cut(s) 10, 139
LmnI GCTCC 2 cut(s) 107, 195
Lsp1109I GCAGC 1 cut(s) 210
MaeI CTAG 1 cut(s) 294
MaeIII GTNAC 3 cut(s) 228, 239, 545
MboII GAAGA 8 cut(s) 61, 365, 376, 434, 580, 588, 612, 627
MluCI AATT 2 cut(s) 437, 537
Mly113I GGCGCC 2 cut(s) 11, 140
MlyI GAGTC 2 cut(s) 54, 112
MseI TTAA 3 cut(s) 441, 492, 525
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 234, 453, 543
MvaI CCWGG 2 cut(s) 453, 543
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 1 cut(s) 148
NarI GGCGCC 2 cut(s) 11, 140
NciI CCSGG 1 cut(s) 234
NlaIII CATG 1 cut(s) 268
NlaIV GGNNCC 3 cut(s) 12, 141, 299
NmeAIII GCCGAG 2 cut(s) 39, 116
NmuCI GTSAC 1 cut(s) 239
PfeI GAWTC 4 cut(s) 96, 476, 581, 671
PkrI GCNGC 3 cut(s) 112, 115, 200
PleI GAGTC 2 cut(s) 54, 112
PluTI GGCGCC 2 cut(s) 14, 143
PpsI GAGTC 2 cut(s) 54, 112
Psp6I CCWGG 2 cut(s) 451, 541
PspGI CCWGG 2 cut(s) 451, 541
PspN4I GGNNCC 3 cut(s) 12, 141, 299
PspPI GGNCC 2 cut(s) 298, 623
PstNI CAGNNNCTG 1 cut(s) 680
RsaI GTAC 3 cut(s) 182, 385, 412
RsaNI GTAC 3 cut(s) 181, 384, 411
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 3 cut(s) 441, 492, 525
SatI GCNGC 3 cut(s) 111, 114, 199
Sau96I GGNCC 2 cut(s) 298, 623
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 2 cut(s) 54, 112
ScrFI CCNGG 3 cut(s) 234, 453, 543
SetI ASST 6 cut(s) 104, 317, 321, 395, 514, 628
SfoI GGCGCC 2 cut(s) 12, 141
SinI GGWCC 2 cut(s) 298, 623
SmiMI CAYNNNNRTG 1 cut(s) 263
Sse9I AATT 2 cut(s) 437, 537
SsiI CCGC 3 cut(s) 108, 111, 114
SspDI GGCGCC 2 cut(s) 10, 139
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 3 cut(s) 232, 451, 541
TaaI ACNGT 2 cut(s) 337, 415
TasI AATT 2 cut(s) 437, 537
TatI WGTACW 2 cut(s) 180, 410
TauI GCSGC 2 cut(s) 113, 116
TfiI GAWTC 4 cut(s) 96, 476, 581, 671
Tru1I TTAA 3 cut(s) 441, 492, 525
Tru9I TTAA 3 cut(s) 441, 492, 525
TscAI CASTG 3 cut(s) 257, 403, 413
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 621
TspRI CASTG 3 cut(s) 257, 403, 413
VpaK11BI GGWCC 2 cut(s) 298, 623
XapI RAATTY 1 cut(s) 437
XspI CTAG 1 cut(s) 294
ZrmI AGTACT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.