MD11G1237900.v1.1

Monodehydroascorbate reductase, seedling isozyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013840
Physical Location & Seq
Forward (+)
34581530 .. 34583224
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1237900.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGGGATTTCTGACACCGATTTATGAACGAATAATGTTCATATGGGCTCTGATTACACGATGGTTAAAGTATCGACAAAACTTTGAAGTTAATAATATGAACACTCTAAGAGGAATGTTGGAAGAGCTAATTAGCCAAAGTGAAGATATAATCGAAAAACTAGAGGCCGAGCCTCTCCTTCCAAGTAAACCACAATGCGATGAAGTCTACGAGTGGTTAGAGAACGTGGAAAGGATCAAAACTCAAATACTAACTATTGAAAAACTCGGCGAAAGGAAATTTCTCTCGATTGCACATCTTGGAAAGCTTGTTGATGAAAAAATTAGAGAAGTTGAAGAATTGCTTGAAAAGGGTAGTCTCTTTAGTGAGATTTCCCTTAAGAAGTCGTTTAAGTACGTGATCATCGGTGGTGGAGTTGCAGCTGGATACGCAGCAAGGGAGTTTGTCAAGCAAGGGCTTGAACCGGGTGAGTTAGCGATCATATCCAAAGAAGCCGTTGCACCTTATGAACGTCCGGCACTTAGTAAGGGCTATCTGCTTCCTGAAGGAGCTACAAGGCTTCCAGGGTTCCATGTCTGTGTCGGAAGTGGAGGAGAGAAGCTGCTTCCGGAGTGGTATGCGGAGAAAGGCATTGTGTTACTTCTTAGCACAGAAATAGTCGAAGCAGATCTTCATTCAAAGATCCTCACTAGTGCAACTGGGGGAATGTTTGAATTCGAAACTTTGATCATTGCAACTGGTTCCAGAGTTATAAGGTTGACAGAATTTGGTGTGCAAGGAGATCATGCCAAAAACATTTTCTACTTGAGGGAAATCAGTGATGCCGATAAACTTGTGGAGGCAATTAGAGCCAAGAAAAACGGAAAGGTAGTGATTGTTGGAGGAGGGTACATTGGTCTTGAAGTCGGAGCAGCTATTAGAATCAACAAGTTCGATGTCACCATGGTTTTTCCGGAACCGTGGTGCATGCCTCGGCTTTTCACTAAAGAGATAGCCGCTTTCTACGAGGGTTACTACGCCAATGAAGGAGTTCAAATGATCAAGGGCACCACTGCAGTAGGGTTCGATGTTGACACAAACGGAGAGGTAAAGGCAGTGAAACTCAAGGATGGTAGGGTGCTGCAAGCTGATATTGTTGTTGTTGGTGTCGGCGCAAAGCCTCTAATTGATTTGTTCAAGGGGCTAGTCGGTGAAGAGAAAGGCGGAATCCAAACTGATGGAATGTTCGAAACAGATGTCCCTGATGTATACGCCGTGGGGGACGTTGCTACTTTCCATATGAAATTGTACGATGACATGAGAAGAGTTGAGCATGTTGACCATGCCAGAAAATCAGCCGAGCATGCTGTGAGAGCTATCAGGGCAAGTGAGGAAGGGAAGTGCATCGAAGAGTACGACTATGTTCCGTACTCCTACTCCCGTGCCTTTGATTTGTCGTGGACGTTTTATGGAGACAATGTAGGTGATCTGTTACTGTTTGGGGACCCCAATCCAGCATCAAGCCAACTGAAGTTTGGAGCAGCATGGATCAAGGACGGGAGGATTGTGGGAGCGTTTTTGGAGGGAGGGACAGATGAAGAAAACAAGGCTATTGCCAAAGTTGCAAGGGCACAACCTTCAGCTGAGTATCTGGATACACTGAAAAGGGAGGGCTCTTCCCTTCATATTTCCATGTTAACAAATTCCCAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Pyr_redox_2

Protein Analysis

565

Amino Acids

62.65

Weight (kDa)

5.2

Isoelectric Point (pI)

28.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 130 - 446 3.3e-53 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 289 - 371 7.8e-14 Pyridine nucleotide-disulphide oxidoreductase
MDHAR3-like_C PF21791 468 - 552 8.7e-34 Monodehydroascorbate reductase 3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013840)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09940 AT3G09940 AT5G03630
fragaria_vesca FvH4_3g20880
malus_domestica MD11G1237900.v1.1
prunus_persica Prupe.4G189300_v2.0.a1
pyrus_communis pycom03g16900 pycom11g20930
rosa_chinensis RchiOBHm_Chr5g0035251
rosa_laevigata RLG00000033613
rosa_multiflora Rmu_co8294091.1_g000001 Rmu_sc0007948.1_g000003
rosa_rugosa Rorug05G0151000
rosa_samantha Rh5BG243400 Rh5CG275100 Rh5DG252600
rosa_wichuraiana Rw5G022280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 752
AccB1I GGYRCC 1 cut(s) 1046
AccI GTMKAC 2 cut(s) 207, 1248
AccIII TCCGGA 2 cut(s) 607, 952
AciI CCGC 3 cut(s) 620, 996, 1203
AclWI GGATC 3 cut(s) 242, 676, 1535
AcsI RAATTY 4 cut(s) 278, 713, 764, 1681
AcuI CTGAAG 3 cut(s) 564, 1529, 1602
AfaI GTAC 5 cut(s) 395, 890, 1289, 1394, 1409
AflII CTTAAG 1 cut(s) 377
AhlI ACTAGT 1 cut(s) 689
AjnI CCWGG 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AluBI AGCT 9 cut(s) 127, 307, 422, 551, 601, 914, 1127, 1355, 1622
AluI AGCT 9 cut(s) 127, 307, 422, 551, 601, 914, 1127, 1355, 1622
Alw26I GTCTC 2 cut(s) 362, 1446
AlwI GGATC 3 cut(s) 242, 676, 1535
Aor13HI TCCGGA 2 cut(s) 607, 952
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 6 cut(s) 419, 431, 601, 911, 1120, 1520
ApoI RAATTY 4 cut(s) 278, 713, 764, 1681
ArsI GACNNNNNNTTYG 2 cut(s) 1221, 1253
Asp700I GAANNNNTTC 1 cut(s) 1029
AspLEI GCGC 1 cut(s) 1154
AspS9I GGNCC 1 cut(s) 1483
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 4 cut(s) 479, 931, 1202, 1475
AsuII TTCGAA 2 cut(s) 717, 1227
AvaII GGWCC 1 cut(s) 1483
BaeGI GKGCMC 2 cut(s) 1049, 1612
BaeI ACNNNNGTAYC 2 cut(s) 880, 913
BanI GGYRCC 1 cut(s) 1046
BanII GRGCYC 2 cut(s) 49, 1655
BbvI GCAGC 6 cut(s) 431, 443, 588, 923, 1107, 1532
BccI CCATC 3 cut(s) 54, 1103, 1211
BceAI ACGGC 2 cut(s) 479, 1238
BciT130I CCWGG 1 cut(s) 564
BciVI GTATCC 2 cut(s) 419, 1627
BclI TGATCA 3 cut(s) 399, 726, 1038
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 2 cut(s) 362, 1446
BcuI ACTAGT 1 cut(s) 689
BfaI CTAG 3 cut(s) 161, 690, 1184
BfmI CTRYAG 1 cut(s) 1053
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 2 cut(s) 419, 1627
BglII AGATCT 1 cut(s) 667
BisI GCNGC 7 cut(s) 420, 432, 602, 912, 996, 1121, 1521
BlsI GCNGC 7 cut(s) 421, 433, 603, 913, 997, 1122, 1522
Bme1390I CCNGG 2 cut(s) 465, 564
Bme18I GGWCC 1 cut(s) 1483
BmgT120I GGNCC 1 cut(s) 1483
BmiI GGNNCC 6 cut(s) 569, 742, 957, 1048, 1484, 1485
BmrFI CCNGG 2 cut(s) 465, 564
BmrI ACTGGG 1 cut(s) 708
BmsI GCATC 3 cut(s) 811, 1392, 1505
BmuI ACTGGG 1 cut(s) 708
Bpu14I TTCGAA 2 cut(s) 717, 1227
BpuEI CTTGAG 2 cut(s) 826, 1088
BpuMI CCSGG 1 cut(s) 465
BsaAI YACGTR 1 cut(s) 397
BsaJI CCNNGG 5 cut(s) 563, 942, 959, 971, 1254
BsaWI WCCGGW 2 cut(s) 607, 952
BsaXI ACNNNNNCTCC 4 cut(s) 1400, 1430, 1443, 1473
Bse1I ACTGG 2 cut(s) 703, 742
Bse3DI GCAATG 1 cut(s) 729
BseAI TCCGGA 2 cut(s) 607, 952
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 5 cut(s) 563, 942, 959, 971, 1254
BseGI GGATG 1 cut(s) 1114
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 1 cut(s) 1614
BseNI ACTGG 2 cut(s) 703, 742
BseRI GAGGAG 2 cut(s) 606, 897
BseSI GKGCMC 2 cut(s) 1049, 1612
BseXI GCAGC 6 cut(s) 431, 443, 588, 923, 1107, 1532
BshFI GGCC 1 cut(s) 167
BshNI GGYRCC 1 cut(s) 1046
BsiSI CCGG 4 cut(s) 464, 515, 608, 953
BslFI GGGAC 4 cut(s) 1223, 1274, 1496, 1582
BsmAI GTCTC 2 cut(s) 362, 1446
BsmFI GGGAC 4 cut(s) 1223, 1274, 1496, 1582
BsnI GGCC 1 cut(s) 167
Bsp119I TTCGAA 2 cut(s) 717, 1227
Bsp1286I GDGCHC 4 cut(s) 49, 1049, 1612, 1655
Bsp13I TCCGGA 2 cut(s) 607, 952
Bsp19I CCATGG 1 cut(s) 942
BspACI CCGC 3 cut(s) 620, 996, 1203
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 1 cut(s) 1615
BspEI TCCGGA 2 cut(s) 607, 952
BspLI GGNNCC 6 cut(s) 569, 742, 957, 1048, 1484, 1485
BspMAI CTGCAG 1 cut(s) 1057
BspPI GGATC 3 cut(s) 242, 676, 1535
BspQI GCTCTTC 2 cut(s) 117, 1660
BspT104I TTCGAA 2 cut(s) 717, 1227
BspT107I GGYRCC 1 cut(s) 1046
BspTI CTTAAG 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 729
BsrI ACTGG 2 cut(s) 703, 742
BssECI CCNNGG 5 cut(s) 563, 942, 959, 971, 1254
BssNAI GTATAC 1 cut(s) 1249
BssT1I CCWWGG 1 cut(s) 942
Bst1107I GTATAC 1 cut(s) 1249
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 2 cut(s) 960, 1476
Bst6I CTCTTC 5 cut(s) 117, 1188, 1297, 1383, 1660
BstAFI CTTAAG 1 cut(s) 377
BstBAI YACGTR 1 cut(s) 397
BstBI TTCGAA 2 cut(s) 717, 1227
BstC8I GCNNGC 3 cut(s) 968, 1125, 1344
BstDEI CTNAG 4 cut(s) 107, 521, 644, 1623
BstDSI CCRYGG 3 cut(s) 942, 959, 1254
BstF5I GGATG 1 cut(s) 1114
BstHHI GCGC 1 cut(s) 1154
BstMAI GTCTC 2 cut(s) 362, 1446
BstMWI GCNNNNNNNGC 6 cut(s) 428, 848, 1343, 1352, 1361, 1601
BstNI CCWGG 1 cut(s) 564
BstNSI RCATGY 3 cut(s) 970, 1316, 1346
BstSCI CCNGG 2 cut(s) 463, 562
BstSFI CTRYAG 1 cut(s) 1053
BstSLI GKGCMC 2 cut(s) 1049, 1612
BstV1I GCAGC 6 cut(s) 431, 443, 588, 923, 1107, 1532
BstX2I RGATCY 2 cut(s) 667, 681
BstXI CCANNNNNNTGG 1 cut(s) 1217
BstYI RGATCY 2 cut(s) 667, 681
BstZ17I GTATAC 1 cut(s) 1249
BsuI GTATCC 2 cut(s) 419, 1627
BsuRI GGCC 1 cut(s) 167
BtgI CCRYGG 3 cut(s) 942, 959, 1254
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 1 cut(s) 1114
BtsI GCAGTG 2 cut(s) 1050, 1101
BtsIMutI CAGTG 4 cut(s) 823, 1050, 1101, 1637
Cac8I GCNNGC 3 cut(s) 968, 1125, 1344
CfoI GCGC 1 cut(s) 1154
Cfr13I GGNCC 1 cut(s) 1483
Csp6I GTAC 5 cut(s) 394, 889, 1288, 1393, 1408
CviQI GTAC 5 cut(s) 394, 889, 1288, 1393, 1408
DdeI CTNAG 4 cut(s) 107, 521, 644, 1623
Eam1104I CTCTTC 5 cut(s) 117, 1188, 1297, 1383, 1660
EarI CTCTTC 5 cut(s) 117, 1188, 1297, 1383, 1660
EciI GGCGGA 1 cut(s) 1218
Eco130I CCWWGG 1 cut(s) 942
Eco24I GRGCYC 2 cut(s) 49, 1655
Eco47I GGWCC 1 cut(s) 1483
Eco57I CTGAAG 3 cut(s) 564, 1529, 1602
EcoO109I RGGNCCY 1 cut(s) 1483
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 942
EcoT38I GRGCYC 2 cut(s) 49, 1655
ErhI CCWWGG 1 cut(s) 942
FalI AAGNNNNNCTT 4 cut(s) 327, 359, 654, 686
FaqI GGGAC 4 cut(s) 1223, 1274, 1496, 1582
FauNDI CATATG 2 cut(s) 41, 1278
FbaI TGATCA 3 cut(s) 399, 726, 1038
FblI GTMKAC 2 cut(s) 207, 1248
Fnu4HI GCNGC 7 cut(s) 420, 432, 602, 912, 996, 1121, 1521
FokI GGATG 1 cut(s) 1121
FriOI GRGCYC 2 cut(s) 49, 1655
Fsp4HI GCNGC 7 cut(s) 420, 432, 602, 912, 996, 1121, 1521
FspBI CTAG 3 cut(s) 161, 690, 1184
GlaI GCGC 1 cut(s) 1153
GluI GCNGC 7 cut(s) 420, 432, 602, 912, 996, 1121, 1521
HaeIII GGCC 1 cut(s) 167
HapII CCGG 4 cut(s) 464, 515, 608, 953
HhaI GCGC 1 cut(s) 1154
Hin6I GCGC 1 cut(s) 1152
HinP1I GCGC 1 cut(s) 1152
HincII GTYRAC 4 cut(s) 759, 1072, 1318, 1677
HindII GTYRAC 4 cut(s) 759, 1072, 1318, 1677
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 2 cut(s) 921, 1206
HpaI GTTAAC 1 cut(s) 1677
HpaII CCGG 4 cut(s) 464, 515, 608, 953
HphI GGTGA 4 cut(s) 479, 931, 1202, 1475
Hpy166II GTNNAC 8 cut(s) 188, 208, 759, 1072, 1249, 1318, 1440, 1677
Hpy188I TCNGA 4 cut(s) 12, 51, 584, 908
Hpy188III TCNNGA 7 cut(s) 286, 542, 608, 744, 899, 953, 1631
Hpy8I GTNNAC 8 cut(s) 188, 208, 759, 1072, 1249, 1318, 1440, 1677
HpyAV CCTTC 6 cut(s) 188, 539, 1019, 1367, 1626, 1670
HpyCH4III ACNGT 2 cut(s) 960, 1476
HpyCH4IV ACGT 5 cut(s) 225, 396, 511, 1263, 1442
HpyF10VI GCNNNNNNNGC 6 cut(s) 428, 848, 1343, 1352, 1361, 1601
HpyF3I CTNAG 4 cut(s) 107, 521, 644, 1623
HpySE526I ACGT 5 cut(s) 225, 396, 511, 1263, 1442
HspAI GCGC 1 cut(s) 1152
KflI GGGWCCC 1 cut(s) 1483
Kpn2I TCCGGA 2 cut(s) 607, 952
Ksp22I TGATCA 3 cut(s) 399, 726, 1038
KspAI GTTAAC 1 cut(s) 1677
LguI GCTCTTC 2 cut(s) 117, 1660
LmnI GCTCC 4 cut(s) 548, 908, 1517, 1550
Lsp1109I GCAGC 6 cut(s) 431, 443, 588, 923, 1107, 1532
LweI GCATC 3 cut(s) 811, 1392, 1505
MaeI CTAG 3 cut(s) 161, 690, 1184
MaeII ACGT 5 cut(s) 225, 396, 511, 1263, 1442
MaeIII GTNAC 4 cut(s) 636, 937, 1010, 1470
MboII GAAGA 9 cut(s) 134, 155, 347, 662, 1205, 1314, 1400, 1589, 1647
MflI RGATCY 2 cut(s) 667, 681
MhlI GDGCHC 4 cut(s) 49, 1049, 1612, 1655
MmeI TCCRAC 4 cut(s) 99, 562, 859, 886
MroI TCCGGA 2 cut(s) 607, 952
MroXI GAANNNNTTC 1 cut(s) 1029
MseI TTAA 5 cut(s) 65, 90, 378, 390, 1676
MslI CAYNNNNRTG 1 cut(s) 576
MspA1I CMGCKG 2 cut(s) 422, 1622
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 4 cut(s) 464, 515, 608, 953
MspR9I CCNGG 2 cut(s) 465, 564
MvaI CCWGG 1 cut(s) 564
MwoI GCNNNNNNNGC 6 cut(s) 428, 848, 1343, 1352, 1361, 1601
NciI CCSGG 1 cut(s) 465
NcoI CCATGG 1 cut(s) 942
NdeI CATATG 2 cut(s) 41, 1278
NlaIV GGNNCC 6 cut(s) 569, 742, 957, 1048, 1484, 1485
NmeAIII GCCGAG 4 cut(s) 193, 246, 952, 1363
NmuCI GTSAC 1 cut(s) 937
NspI RCATGY 3 cut(s) 970, 1316, 1346
NspV TTCGAA 2 cut(s) 717, 1227
PaeI GCATGC 2 cut(s) 970, 1346
PciSI GCTCTTC 2 cut(s) 117, 1660
PcsI WCGNNNNNNNCGW 1 cut(s) 1392
PdmI GAANNNNTTC 1 cut(s) 1029
PfeI GAWTC 2 cut(s) 921, 1206
PkrI GCNGC 7 cut(s) 421, 433, 603, 913, 997, 1122, 1522
Ppu21I YACGTR 1 cut(s) 397
PpuMI RGGWCCY 1 cut(s) 1483
PsiI TTATAA 1 cut(s) 752
Psp5II RGGWCCY 1 cut(s) 1483
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 6 cut(s) 569, 742, 957, 1048, 1484, 1485
PspPI GGNCC 1 cut(s) 1483
PspPPI RGGWCCY 1 cut(s) 1483
PstI CTGCAG 1 cut(s) 1057
PsuI RGATCY 2 cut(s) 667, 681
PvuII CAGCTG 2 cut(s) 422, 1622
RsaI GTAC 5 cut(s) 395, 890, 1289, 1394, 1409
RsaNI GTAC 5 cut(s) 394, 889, 1288, 1393, 1408
RseI CAYNNNNRTG 1 cut(s) 576
SapI GCTCTTC 2 cut(s) 117, 1660
SaqAI TTAA 5 cut(s) 65, 90, 378, 390, 1676
SatI GCNGC 7 cut(s) 420, 432, 602, 912, 996, 1121, 1521
Sau96I GGNCC 1 cut(s) 1483
ScrFI CCNGG 2 cut(s) 465, 564
SduI GDGCHC 4 cut(s) 49, 1049, 1612, 1655
SfaNI GCATC 3 cut(s) 811, 1392, 1505
SfcI CTRYAG 1 cut(s) 1053
SfuI TTCGAA 2 cut(s) 717, 1227
SinI GGWCC 1 cut(s) 1483
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 3 cut(s) 377, 805, 1103
SmoI CTYRAG 3 cut(s) 377, 805, 1103
SpeI ACTAGT 1 cut(s) 689
SphI GCATGC 2 cut(s) 970, 1346
SsiI CCGC 3 cut(s) 620, 996, 1203
SspMI CTAG 3 cut(s) 161, 690, 1184
StyD4I CCNGG 2 cut(s) 463, 562
StyI CCWWGG 1 cut(s) 942
TaaI ACNGT 2 cut(s) 960, 1476
TaiI ACGT 5 cut(s) 228, 399, 514, 1266, 1445
TaqI TCGA 9 cut(s) 73, 153, 287, 660, 717, 933, 1065, 1227, 1386
TauI GCSGC 1 cut(s) 998
TfiI GAWTC 2 cut(s) 921, 1206
Tru1I TTAA 5 cut(s) 65, 90, 378, 390, 1676
Tru9I TTAA 5 cut(s) 65, 90, 378, 390, 1676
TscAI CASTG 4 cut(s) 823, 1057, 1101, 1644
TseFI GTSAC 1 cut(s) 937
TseI GCWGC 6 cut(s) 419, 431, 601, 911, 1120, 1520
Tsp45I GTSAC 1 cut(s) 937
TspGWI ACGGA 3 cut(s) 876, 1095, 1395
TspRI CASTG 4 cut(s) 823, 1057, 1101, 1644
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 1483
XapI RAATTY 4 cut(s) 278, 713, 764, 1681
XceI RCATGY 3 cut(s) 970, 1316, 1346
XcmI CCANNNNNNNNNTGG 1 cut(s) 1511
XmiI GTMKAC 2 cut(s) 207, 1248
XmnI GAANNNNTTC 1 cut(s) 1029
XspI CTAG 3 cut(s) 161, 690, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.