RLG00000033613

Monodehydroascorbate reductase, seedling isozyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013840
Physical Location & Seq
Reverse (-)
29610723 .. 29615610
4888 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033613

Sequence Viewer

Length: 2361 bp
ATGGGGATATGGGATACCATTTCTGGGACAACCGATTCACTGAAACGGAACGCGCCGGATCCAACGGCCTTGAAGAGCTGGTGCCCCACAACCGATTCGCTCAAGCGAATCACACCTGATGTAACAGCTGCCAAGAACGTCTGCTCCACCGCTTACGGTTATGGCTCCGCCGCCGTTACCAAAGTCGACGGCGCCGTCAGGGGTAACTTGATCCCTTACTTTCGAAACGAGGAAGCATGGTCCAAGATGGCCCAATTCGGCACCATCGTTGCTAAAAACACAACCTATGAGGGCCTCAAACTCGTTCCAGGTGGAAGTACATTTTATAACATTGCTGCCGGTTCCATCCGTGATTTTAACAATTCCAATAAGGATGAAGTGGATGTGAAAGCATTGCAAGCTGAACTTTTGAGTCTGAAGAAAGAATTGAGTGAATACCGGAAAATGAATAAGCCATGTGCAGATGTGAAGTCTCCCAGCATAGCCAACGTTCAATCAGTTGCGACTTGTAAACCTGAAGATGTGATCAATGTCTTTATGATGAAGGAGTTCATGGGCAGGCAATTCTGTGGTGATCTATTGGTTCCGGTTGCGCCCAGGAAGAGGGCAGTGACAACTACGAGATTTCTTAGAGGAGAGGCATTGGTGCATTCACCGAAAAGGATTCGCATCAGTGAGGCCAGGAACTTACCTATGGCATTGTTGAAGCAAATTACTGAAAGAATACTAGTCATATGGGCTCTGATTACTAACTGGTCGAAGTATCAAAGAAACCGTGAAGGAAATATGGAGCTTCTGAACAGATTGTTAGAAGAGCTAGATAGCCAAACTGCAGATATAGAGCAAAAGCTAGACGCTGAGCCTCCTCATCCAATCAAAAGACAAGAAGATGAAGTCTATGAGTGGTTGGAAAATGTGGAAAGGATCAAAACTGAAGTGCAAACCATTTACCAAATTGCTGAAAGGAACTATCTCTCACTTGAACATTTCAGGAAGCTTGTTAATGAAAAAATTGTAGAGGTTAAGGAGTTGCTCCAGGAAGGAAAATTGTACACTGGGATTTCCGAGGAGAAGTGTTTCAAGTACTTGATCATTGGTGGTGGAGTTGCAGCTGGATATGCAGCCAGGGAGTTTGTCAATCAAGGGCTCAAGCCAGGTGAGTTAGCAATCATATCAAAAGAAGCAGTGGCGCCTTATGAACGTCCTGCGCTTAGTAAGGCCTACCTGTTTCCTGAGGGAGCTGTAAGACTTCCAGGGTTCCATGTCTGCGTTGGAAGTGGAGGTGAGAGGCTGCTACCTGAGTGGTATGCGGAGAAAGGTATTGAATTAATTCTTAGTACAGAAATTATCAAAGCAGATCTTGATTCGAAGATCCTCACCAGTGCAGCTGGGATGCAATATAGTTATGATATTTTGATAATTGCAACTGGTTCCAGAGCAATAAGGTTGACAGAGTGTGGTGCACACGGAACTGATGCAAAGAACATTTTCTACTTGAGAGAAATCAGTGATGCTGATAAGCTTGTAGAAGAAATTAGAGCCAAGAAAAAGGGAAAGGTAGTGGTTGTTGGAGGAGGATACATTGGTCTTGAAGTTGGAGCTGCTATGAGAATCAACCAGTTTGATGTTACCATAGTTTATCGCGATCAATGGTGCATGGCACGGCTTTTCACTCGAGAGATAGCTGCTTTCTACGAGACTTACTATGAAAACAAAGGAATCAAACTCATCAAAGGTACAGTTGCTGTTGCCTTTGATTCTGGTGCAAATGGAGAGGTAAAAGCAGTCAAACTCAAGGATGGTAGGGTGCTTGAAGCTGACATTGTTGTTGTTGGTGTCGGTGCAAAACCTCTCATTGAGTTATTTGAAGGGCAGCTTGATGAAGCTAAAGATGGAATCAAAACTGATGGATTCTTTGAAACAGATGATCCTGACGTATATGCTGTAGGCGATGTGGCTACTTTCCCTATAAAGTTGTACAATGACATGAGAAGAGTAGAGCATGTTGACCATGCTAGGAAATCAGCAGAGCAAGCAGTAAAAGTTATCAAGGCAAGTGAAGAAAATAGATCTATTCCAGAGTATGATTATCTTCCATACTTCTATTCACGTGTCTTCAATCTTTCGTGGTCGTTTTATGGAGACAATGTGGGTGATCCTGTGATATTTGGAGACAACAATCCGGCATCAGCGAAACCAAAGTTTGGAACAGCATGGATCAAGAATGGGAAGATTGTGGGAGTTTTCTTAGAGGGAGGAACAGCTGAAGAAAACAAGGCTATCTGCCAGATTGCGAGGGTCCAACCTTCAGCTGATAAGTATTTGGATCAGCTGAGAAAGGGTGATTATTTCTTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Pyr_redox_2

Protein Analysis

787

Amino Acids

87.62

Weight (kDa)

6.33

Isoelectric Point (pI)

28.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 361 - 676 3.4e-55 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 519 - 601 3.6e-15 Pyridine nucleotide-disulphide oxidoreductase
MDHAR3-like_C PF21791 698 - 780 7.1e-34 Monodehydroascorbate reductase 3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013840)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09940 AT3G09940 AT5G03630
fragaria_vesca FvH4_3g20880
malus_domestica MD11G1237900.v1.1
prunus_persica Prupe.4G189300_v2.0.a1
pyrus_communis pycom03g16900 pycom11g20930
rosa_chinensis RchiOBHm_Chr5g0035251
rosa_laevigata RLG00000033613
rosa_multiflora Rmu_co8294091.1_g000001 Rmu_sc0007948.1_g000003
rosa_rugosa Rorug05G0151000
rosa_samantha Rh5BG243400 Rh5CG275100 Rh5DG252600
rosa_wichuraiana Rw5G022280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AasI GACNNNNNNGTC 1 cut(s) 194
AccB1I GGYRCC 4 cut(s) 81, 191, 260, 1189
AccB7I CCANNNNNTGG 1 cut(s) 2204
AccI GTMKAC 1 cut(s) 186
AccII CGCG 2 cut(s) 53, 1644
AciI CCGC 4 cut(s) 150, 168, 171, 1310
AclI AACGTT 1 cut(s) 489
AclWI GGATC 9 cut(s) 53, 66, 205, 932, 1366, 1922, 2150, 2225, 2334
AcuI CTGAAG 5 cut(s) 437, 537, 954, 2286, 2292
AcvI CACGTG 1 cut(s) 2111
AcyI GRCGYC 2 cut(s) 192, 1190
AfaI GTAC 6 cut(s) 319, 1052, 1085, 1339, 1738, 1979
AfiI CCNNNNNNNGG 3 cut(s) 24, 603, 2204
AflIII ACRYGT 1 cut(s) 2110
AhlI ACTAGT 1 cut(s) 727
AjnI CCWGG 7 cut(s) 307, 596, 680, 1035, 1124, 1153, 1252
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
Alw21I GWGCWC 1 cut(s) 1465
Alw26I GTCTC 4 cut(s) 477, 1691, 2136, 2166
Alw44I GTGCAC 1 cut(s) 1461
AlwI GGATC 9 cut(s) 53, 66, 205, 932, 1366, 1922, 2150, 2225, 2334
AlwNI CAGNNNCTG 1 cut(s) 1745
Ama87I CYCGRG 1 cut(s) 1674
AoxI GGCC 5 cut(s) 66, 249, 292, 678, 1218
ApaLI GTGCAC 1 cut(s) 1461
ApeKI GCWGC 9 cut(s) 128, 335, 1109, 1121, 1291, 1385, 1601, 1685, 1873
AseI ATTAAT 1 cut(s) 1328
Asp700I GAANNNNTTC 4 cut(s) 548, 1076, 1329, 1487
AspLEI GCGC 5 cut(s) 55, 194, 595, 1192, 1210
AspS9I GGNCC 4 cut(s) 240, 250, 292, 2299
AsuHPI GGTGA 7 cut(s) 584, 645, 1169, 1295, 1369, 2165, 2354
AsuII TTCGAA 2 cut(s) 223, 1367
AvaI CYCGRG 1 cut(s) 1674
AvaII GGWCC 2 cut(s) 240, 2299
AxyI CCTNAGG 1 cut(s) 1233
BaeGI GKGCMC 2 cut(s) 86, 1465
BaeI ACNNNNGTAYC 2 cut(s) 1570, 1603
BamHI GGATCC 1 cut(s) 58
BanI GGYRCC 4 cut(s) 81, 191, 260, 1189
BanII GRGCYC 2 cut(s) 742, 1149
BbrPI CACGTG 1 cut(s) 2111
BbsI GAAGAC 1 cut(s) 2107
Bbv12I GWGCWC 1 cut(s) 1465
BbvI GCAGC 9 cut(s) 115, 322, 1121, 1133, 1278, 1397, 1588, 1672, 1885
BccI CCATC 6 cut(s) 241, 272, 353, 1793, 1886, 1901
BceAI ACGGC 5 cut(s) 81, 158, 179, 205, 1679
BciT130I CCWGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
BciVI GTATCC 2 cut(s) 7, 1571
BclI TGATCA 2 cut(s) 525, 1089
BcoDI GTCTC 4 cut(s) 477, 1691, 2136, 2166
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 4 cut(s) 728, 818, 851, 2016
BfmI CTRYAG 2 cut(s) 831, 1944
BfoI RGCGCY 2 cut(s) 195, 1193
BfuI GTATCC 2 cut(s) 7, 1571
BglII AGATCT 2 cut(s) 1357, 2069
BlpI GCTNAGC 1 cut(s) 858
BmcAI AGTACT 1 cut(s) 1085
Bme1390I CCNGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
Bme18I GGWCC 2 cut(s) 240, 2299
BmeT110I CYCGRG 1 cut(s) 1674
BmgT120I GGNCC 4 cut(s) 240, 250, 292, 2299
BmrFI CCNGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
BmrI ACTGGG 1 cut(s) 1065
BmsI GCATC 5 cut(s) 678, 1383, 1465, 1501, 2195
BmuI ACTGGG 1 cut(s) 1065
BpiI GAAGAC 1 cut(s) 2107
BpmI CTGGAG 1 cut(s) 1019
Bpu1102I GCTNAGC 1 cut(s) 858
Bpu14I TTCGAA 2 cut(s) 223, 1367
BpuEI CTTGAG 4 cut(s) 86, 1133, 1516, 1778
BsaAI YACGTR 1 cut(s) 2111
BsaBI GATNNNNATC 1 cut(s) 668
BsaHI GRCGYC 2 cut(s) 192, 1190
BsaJI CCNNGG 4 cut(s) 596, 1065, 1125, 1253
BsaWI WCCGGW 2 cut(s) 438, 586
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 2133, 2163
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 603, 2204
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 5 cut(s) 758, 1060, 1380, 1432, 1618
Bse21I CCTNAGG 1 cut(s) 1233
Bse3DI GCAATG 2 cut(s) 330, 392
Bse8I GATNNNNATC 1 cut(s) 668
BseBI CCWGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
BseDI CCNNGG 4 cut(s) 596, 1065, 1125, 1253
BseGI GGATG 6 cut(s) 345, 379, 388, 868, 1398, 1804
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 3 cut(s) 24, 603, 2204
BseMI GCAATG 2 cut(s) 330, 392
BseMII CTCAG 4 cut(s) 849, 1224, 1290, 2324
BseNI ACTGG 5 cut(s) 758, 1060, 1380, 1432, 1618
BseRI GAGGAG 4 cut(s) 648, 855, 1082, 1587
BseSI GKGCMC 2 cut(s) 86, 1465
BseXI GCAGC 9 cut(s) 115, 322, 1121, 1133, 1278, 1397, 1588, 1672, 1885
BseYI CCCAGC 2 cut(s) 476, 1388
BsgI GTGCAG 2 cut(s) 480, 1404
Bsh1236I CGCG 2 cut(s) 53, 1644
BshFI GGCC 5 cut(s) 68, 251, 294, 680, 1220
BshNI GGYRCC 4 cut(s) 81, 191, 260, 1189
BsiHKAI GWGCWC 1 cut(s) 1465
BsiHKCI CYCGRG 1 cut(s) 1674
BsiSI CCGG 5 cut(s) 56, 339, 439, 587, 2183
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 3 cut(s) 24, 603, 2204
BsmAI GTCTC 4 cut(s) 477, 1691, 2136, 2166
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 5 cut(s) 68, 251, 294, 680, 1220
BsoBI CYCGRG 1 cut(s) 1674
Bsp119I TTCGAA 2 cut(s) 223, 1367
Bsp1286I GDGCHC 4 cut(s) 86, 742, 1149, 1465
Bsp1407I TGTACA 2 cut(s) 1050, 1977
Bsp1720I GCTNAGC 1 cut(s) 858
Bsp68I TCGCGA 1 cut(s) 1644
BspACI CCGC 4 cut(s) 150, 168, 171, 1310
BspANI GGCC 5 cut(s) 68, 251, 294, 680, 1220
BspCNI CTCAG 4 cut(s) 850, 1225, 1291, 2325
BspFNI CGCG 2 cut(s) 53, 1644
BspMAI CTGCAG 1 cut(s) 835
BspPI GGATC 9 cut(s) 53, 66, 205, 932, 1366, 1922, 2150, 2225, 2334
BspQI GCTCTTC 2 cut(s) 68, 807
BspT104I TTCGAA 2 cut(s) 223, 1367
BspT107I GGYRCC 4 cut(s) 81, 191, 260, 1189
BsrDI GCAATG 2 cut(s) 330, 392
BsrFI RCCGGY 1 cut(s) 338
BsrGI TGTACA 2 cut(s) 1050, 1977
BsrI ACTGG 5 cut(s) 758, 1060, 1380, 1432, 1618
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 4 cut(s) 596, 1065, 1125, 1253
BssNI GRCGYC 2 cut(s) 192, 1190
Bst2UI CCWGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
Bst4CI ACNGT 3 cut(s) 158, 776, 1741
Bst6I CTCTTC 4 cut(s) 68, 596, 807, 1987
BstACI GRCGYC 2 cut(s) 192, 1190
BstAUI TGTACA 2 cut(s) 1050, 1977
BstBAI YACGTR 1 cut(s) 2111
BstBI TTCGAA 2 cut(s) 223, 1367
BstC8I GCNNGC 3 cut(s) 399, 560, 2034
BstDEI CTNAG 8 cut(s) 629, 858, 1211, 1233, 1299, 1334, 2248, 2333
BstF5I GGATG 6 cut(s) 345, 379, 388, 868, 1398, 1804
BstFNI CGCG 2 cut(s) 53, 1644
BstH2I RGCGCY 2 cut(s) 195, 1193
BstHHI GCGC 5 cut(s) 55, 194, 595, 1192, 1210
BstMAI GTCTC 4 cut(s) 477, 1691, 2136, 2166
BstMWI GCNNNNNNNGC 3 cut(s) 398, 1118, 2033
BstNI CCWGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
BstNSI RCATGY 1 cut(s) 2006
BstSCI CCNGG 7 cut(s) 307, 596, 680, 1035, 1124, 1153, 1252
BstSFI CTRYAG 2 cut(s) 831, 1944
BstSLI GKGCMC 2 cut(s) 86, 1465
BstUI CGCG 2 cut(s) 53, 1644
BstV1I GCAGC 9 cut(s) 115, 322, 1121, 1133, 1278, 1397, 1588, 1672, 1885
BstV2I GAAGAC 1 cut(s) 2107
BstX2I RGATCY 4 cut(s) 58, 1357, 1371, 2069
BstYI RGATCY 4 cut(s) 58, 1357, 1371, 2069
Bsu36I CCTNAGG 1 cut(s) 1233
BsuI GTATCC 2 cut(s) 7, 1571
BsuRI GGCC 5 cut(s) 68, 251, 294, 680, 1220
BtgZI GCGATG 1 cut(s) 1965
BtsCI GGATG 6 cut(s) 345, 379, 388, 868, 1398, 1804
BtsI GCAGTG 2 cut(s) 615, 1191
BtsIMutI CAGTG 7 cut(s) 38, 615, 679, 1053, 1191, 1387, 1513
BtuMI TCGCGA 1 cut(s) 1644
Cac8I GCNNGC 3 cut(s) 399, 560, 2034
CaiI CAGNNNCTG 1 cut(s) 1745
CfoI GCGC 5 cut(s) 55, 194, 595, 1192, 1210
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 4 cut(s) 240, 250, 292, 2299
CseI GACGC 1 cut(s) 863
Csp6I GTAC 6 cut(s) 318, 1051, 1084, 1338, 1737, 1978
CviAII CATG 9 cut(s) 237, 456, 553, 1262, 1657, 1987, 2003, 2012, 2214
CviQI GTAC 6 cut(s) 318, 1051, 1084, 1338, 1737, 1978
DdeI CTNAG 8 cut(s) 629, 858, 1211, 1233, 1299, 1334, 2248, 2333
DinI GGCGCC 2 cut(s) 193, 1191
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
Eam1104I CTCTTC 4 cut(s) 68, 596, 807, 1987
EarI CTCTTC 4 cut(s) 68, 596, 807, 1987
EciI GGCGGA 1 cut(s) 157
Eco147I AGGCCT 1 cut(s) 1220
Eco24I GRGCYC 2 cut(s) 742, 1149
Eco47I GGWCC 2 cut(s) 240, 2299
Eco57I CTGAAG 5 cut(s) 437, 537, 954, 2286, 2292
Eco72I CACGTG 1 cut(s) 2111
Eco81I CCTNAGG 1 cut(s) 1233
Eco88I CYCGRG 1 cut(s) 1674
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 7 cut(s) 307, 596, 680, 1035, 1124, 1153, 1252
EcoT38I GRGCYC 2 cut(s) 742, 1149
EgeI GGCGCC 2 cut(s) 193, 1191
EheI GGCGCC 2 cut(s) 193, 1191
FaeI CATG 9 cut(s) 240, 459, 556, 1265, 1660, 1990, 2006, 2015, 2217
FalI AAGNNNNNCTT 6 cut(s) 390, 422, 1344, 1376, 1860, 1892
FaqI GGGAC 1 cut(s) 40
FatI CATG 9 cut(s) 236, 455, 552, 1261, 1656, 1986, 2002, 2011, 2213
FauNDI CATATG 1 cut(s) 734
FbaI TGATCA 2 cut(s) 525, 1089
FblI GTMKAC 1 cut(s) 186
FokI GGATG 6 cut(s) 332, 386, 395, 855, 1405, 1811
FriOI GRGCYC 2 cut(s) 742, 1149
FspBI CTAG 4 cut(s) 728, 818, 851, 2016
GlaI GCGC 5 cut(s) 54, 193, 594, 1191, 1209
GsaI CCCAGC 2 cut(s) 480, 1392
GsuI CTGGAG 1 cut(s) 1019
HaeII RGCGCY 2 cut(s) 195, 1193
HaeIII GGCC 5 cut(s) 68, 251, 294, 680, 1220
HapII CCGG 5 cut(s) 56, 339, 439, 587, 2183
HgaI GACGC 1 cut(s) 863
HhaI GCGC 5 cut(s) 55, 194, 595, 1192, 1210
Hin1I GRCGYC 2 cut(s) 192, 1190
Hin1II CATG 9 cut(s) 240, 459, 556, 1265, 1660, 1990, 2006, 2015, 2217
Hin6I GCGC 5 cut(s) 53, 192, 593, 1190, 1208
HinP1I GCGC 5 cut(s) 53, 192, 593, 1190, 1208
HincII GTYRAC 3 cut(s) 187, 1449, 2008
HindII GTYRAC 3 cut(s) 187, 1449, 2008
HindIII AAGCTT 2 cut(s) 995, 1520
HpaII CCGG 5 cut(s) 56, 339, 439, 587, 2183
HphI GGTGA 7 cut(s) 584, 645, 1169, 1295, 1369, 2165, 2354
Hpy166II GTNNAC 6 cut(s) 187, 512, 1053, 1449, 1463, 2008
Hpy188I TCNGA 4 cut(s) 417, 744, 798, 1066
Hpy8I GTNNAC 6 cut(s) 187, 512, 1053, 1449, 1463, 2008
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 5 cut(s) 538, 773, 1034, 1862, 2316
HpyCH4III ACNGT 3 cut(s) 158, 776, 1741
HpyCH4IV ACGT 5 cut(s) 138, 489, 1201, 1935, 2110
HpyF10VI GCNNNNNNNGC 3 cut(s) 398, 1118, 2033
HpyF3I CTNAG 8 cut(s) 629, 858, 1211, 1233, 1299, 1334, 2248, 2333
HpySE526I ACGT 5 cut(s) 138, 489, 1201, 1935, 2110
Hsp92I GRCGYC 2 cut(s) 192, 1190
Hsp92II CATG 9 cut(s) 240, 459, 556, 1265, 1660, 1990, 2006, 2015, 2217
HspAI GCGC 5 cut(s) 53, 192, 593, 1190, 1208
KasI GGCGCC 2 cut(s) 191, 1189
Ksp22I TGATCA 2 cut(s) 525, 1089
LguI GCTCTTC 2 cut(s) 68, 807
LmnI GCTCC 6 cut(s) 149, 170, 790, 1038, 1238, 1598
Lsp1109I GCAGC 9 cut(s) 115, 322, 1121, 1133, 1278, 1397, 1588, 1672, 1885
LweI GCATC 5 cut(s) 678, 1383, 1465, 1501, 2195
MaeI CTAG 4 cut(s) 728, 818, 851, 2016
MaeII ACGT 5 cut(s) 138, 489, 1201, 1935, 2110
MaeIII GTNAC 5 cut(s) 121, 175, 203, 610, 1627
MflI RGATCY 4 cut(s) 58, 1357, 1371, 2069
MhlI GDGCHC 4 cut(s) 86, 742, 1149, 1465
Mly113I GGCGCC 2 cut(s) 192, 1190
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 6 cut(s) 86, 888, 1252, 1549, 1576, 2326
MroXI GAANNNNTTC 4 cut(s) 548, 1076, 1329, 1487
MseI TTAA 4 cut(s) 357, 1002, 1023, 1328
MspA1I CMGCKG 6 cut(s) 128, 1112, 1388, 2264, 2312, 2332
MspI CCGG 5 cut(s) 56, 339, 439, 587, 2183
MspR9I CCNGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
MvnI CGCG 2 cut(s) 53, 1644
MwoI GCNNNNNNNGC 3 cut(s) 398, 1118, 2033
NarI GGCGCC 2 cut(s) 192, 1190
NdeI CATATG 1 cut(s) 734
NlaIII CATG 9 cut(s) 240, 459, 556, 1265, 1660, 1990, 2006, 2015, 2217
NmuCI GTSAC 1 cut(s) 610
NruI TCGCGA 1 cut(s) 1644
NspI RCATGY 1 cut(s) 2006
NspV TTCGAA 2 cut(s) 223, 1367
PaeR7I CTCGAG 1 cut(s) 1674
PceI AGGCCT 1 cut(s) 1220
PciSI GCTCTTC 2 cut(s) 68, 807
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 264
PctI GAATGC 1 cut(s) 649
PdmI GAANNNNTTC 4 cut(s) 548, 1076, 1329, 1487
PflMI CCANNNNNTGG 1 cut(s) 2204
PfoI TCCNGGA 1 cut(s) 1035
PleI GAGTC 1 cut(s) 420
PluTI GGCGCC 2 cut(s) 195, 1193
PmaCI CACGTG 1 cut(s) 2111
PmlI CACGTG 1 cut(s) 2111
PpsI GAGTC 1 cut(s) 420
Ppu21I YACGTR 1 cut(s) 2111
PshBI ATTAAT 1 cut(s) 1328
PsiI TTATAA 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 489
Psp6I CCWGG 7 cut(s) 307, 596, 680, 1035, 1124, 1153, 1252
PspCI CACGTG 1 cut(s) 2111
PspFI CCCAGC 2 cut(s) 476, 1388
PspGI CCWGG 7 cut(s) 307, 596, 680, 1035, 1124, 1153, 1252
PspPI GGNCC 4 cut(s) 240, 250, 292, 2299
PsrI GAACNNNNNNTAC 2 cut(s) 1475, 1507
PstI CTGCAG 1 cut(s) 835
PstNI CAGNNNCTG 1 cut(s) 1745
PsuI RGATCY 4 cut(s) 58, 1357, 1371, 2069
PvuII CAGCTG 6 cut(s) 128, 1112, 1388, 2264, 2312, 2332
RruI TCGCGA 1 cut(s) 1644
RsaI GTAC 6 cut(s) 319, 1052, 1085, 1339, 1738, 1979
RsaNI GTAC 6 cut(s) 318, 1051, 1084, 1338, 1737, 1978
SalI GTCGAC 1 cut(s) 185
SapI GCTCTTC 2 cut(s) 68, 807
SaqAI TTAA 4 cut(s) 357, 1002, 1023, 1328
Sau96I GGNCC 4 cut(s) 240, 250, 292, 2299
ScaI AGTACT 1 cut(s) 1085
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 7 cut(s) 309, 598, 682, 1037, 1126, 1155, 1254
SduI GDGCHC 4 cut(s) 86, 742, 1149, 1465
SfaNI GCATC 5 cut(s) 678, 1383, 1465, 1501, 2195
SfcI CTRYAG 2 cut(s) 831, 1944
SfoI GGCGCC 2 cut(s) 193, 1191
Sfr274I CTCGAG 1 cut(s) 1674
SfuI TTCGAA 2 cut(s) 223, 1367
SinI GGWCC 2 cut(s) 240, 2299
SlaI CTCGAG 1 cut(s) 1674
SmlI CTYRAG 5 cut(s) 101, 1148, 1495, 1674, 1793
SmoI CTYRAG 5 cut(s) 101, 1148, 1495, 1674, 1793
SpeI ACTAGT 1 cut(s) 727
SseBI AGGCCT 1 cut(s) 1220
SsiI CCGC 4 cut(s) 150, 168, 171, 1310
SspDI GGCGCC 2 cut(s) 191, 1189
SspMI CTAG 4 cut(s) 728, 818, 851, 2016
StuI AGGCCT 1 cut(s) 1220
StyD4I CCNGG 7 cut(s) 307, 596, 680, 1035, 1124, 1153, 1252
TaaI ACNGT 3 cut(s) 158, 776, 1741
TaiI ACGT 5 cut(s) 141, 492, 1204, 1938, 2113
TaqI TCGA 5 cut(s) 186, 223, 758, 1367, 1675
TatI WGTACW 5 cut(s) 317, 1050, 1083, 1337, 1977
TauI GCSGC 1 cut(s) 173
Tru1I TTAA 4 cut(s) 357, 1002, 1023, 1328
Tru9I TTAA 4 cut(s) 357, 1002, 1023, 1328
TscAI CASTG 7 cut(s) 45, 615, 679, 1060, 1191, 1387, 1513
TseFI GTSAC 1 cut(s) 610
TseI GCWGC 9 cut(s) 128, 335, 1109, 1121, 1291, 1385, 1601, 1685, 1873
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 9 cut(s) 390, 461, 541, 557, 906, 1020, 1212, 1722, 1896
TspGWI ACGGA 3 cut(s) 61, 338, 1482
TspRI CASTG 7 cut(s) 45, 615, 679, 1060, 1191, 1387, 1513
Van91I CCANNNNNTGG 1 cut(s) 2204
VneI GTGCAC 1 cut(s) 1461
VpaK11BI GGWCC 2 cut(s) 240, 2299
VspI ATTAAT 1 cut(s) 1328
XceI RCATGY 1 cut(s) 2006
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XhoI CTCGAG 1 cut(s) 1674
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 4 cut(s) 548, 1076, 1329, 1487
XspI CTAG 4 cut(s) 728, 818, 851, 2016
ZrmI AGTACT 1 cut(s) 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.