pycom11g20930

Monodehydroascorbate reductase, seedling isozyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0013840
Physical Location & Seq
Forward (+)
24073438 .. 24074844
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g20930.1

Sequence Viewer

Length: 1407 bp
ATGTTCATATGGGCTCTGATTACACGATGGTTAAAGTATCAACAAAACTTTGAAGTTACTAATATGAACACTCTAAGAGAAATGTTGGAAGAGCTAATTAGCCAAAGTGAAGATGTAATCGAAAAAATAGAGGCCGAGTCTTTCCTTCCAAGTAAACCACAACGGGATGAAGTCTACGAGTGGTTAGAGAATGTGGAAAGGATCAATACTCAAATACTAACTATTGAAAAACTCGGCGAACGGAAATTTCTCTCGATTGCACATCTTGCAAAGCTTGTTGATGACAAAATTGGAGAAGTTGAAGAATTGCTCGAAAAGGGTAGTCTCTTTAGTGAGATTTCCAGTAAGAAGTCGTTTAAGTACGTGATCATCGGTGGTGGAGTTGCAGCTGGATACGCAGCAAGGGAGTTTGTCAAGCAAGGGCTTGAACCTGGTGAGTTAGCGATCATATCCAAAGAAGCCGTTGCACCTTATGAACGTCCGGCACTTAGTAAGGGCTATCTGCTTCCTGAGGGAGCTGCAAGGCTTCCGGGGTTCCATGTGTGTGTTGGAAGTGGAGGAGAGAAGCTGCTTCCGGAGTGGTATGCGGAGAAAGGCATTGAATTACTTCTTAGCACAGAAATAGTCGAAGCAGATCTTCATTCAAAGATCCTCACTAGTGCAACTGGGGGAATGTTTGAATTCGAAACTTTGATCATTGCAACTGGTTCCAGAGTTATAAGGTTGACAGAATTTGGTGTGCAAGGAGATGATACCAAAAACATTTTCTACTTGAGGGAAATCAGCGATGCCGATAAACTTGTGGAGGCAATTAGAGCCAAGAAAAACGGAAAGGTAGTGATTGTTGGAGGAGGGTACATTGGTCTCGAAGTCGGAGCAGCTATTAGAATCAACAAATTCAATGTCACCATGGTTTTTCCGGAACCGTGGTGCATGCCTCGGCTTTTCACTAAAGAGATAGCCGCTTTCTACGAGGGTTACTACGCCAATAGAGGAGTTCAAATGATCAAGGGCACCACTGCAGTAGGGTTCGATGCTGACACGAACGGAGAGGTAAAGGCAGTGAAACTCAAGGATGGTAGGGTGCTGCAAGCTGACATTGTTGTTGTTGGTGTCGGCGCAAAGCCTCTAATTGATTTGTTCAAAGGGCAAGTCGGTGAAGAGAAAGGCGGAATCCAAACTGATGGAATGTTCGAAACAGATGTCCCTGATGTATACTCCGTGGGGGACATTGCTACTTTCCCTATGAAATTGTATGACGACATGAGAAGAGTTGAGCATGTTGACCATGCCAGAAAATCAGCCGAGCATGCTGTGAGAGCTATCAGGGCAAGCGAGGAAGGGAAGTGGATTGAAGAGTATGACTATGTGCCGTACTTCTACTCCCATGCCTTTGATTTGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Pyr_redox_2

Protein Analysis

469

Amino Acids

52.24

Weight (kDa)

5.07

Isoelectric Point (pI)

30.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 119 - 435 1.3e-53 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 278 - 360 7.8e-14 Pyridine nucleotide-disulphide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013840)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09940 AT3G09940 AT5G03630
fragaria_vesca FvH4_3g20880
malus_domestica MD11G1237900.v1.1
prunus_persica Prupe.4G189300_v2.0.a1
pyrus_communis pycom03g16900 pycom11g20930
rosa_chinensis RchiOBHm_Chr5g0035251
rosa_laevigata RLG00000033613
rosa_multiflora Rmu_co8294091.1_g000001 Rmu_sc0007948.1_g000003
rosa_rugosa Rorug05G0151000
rosa_samantha Rh5BG243400 Rh5CG275100 Rh5DG252600
rosa_wichuraiana Rw5G022280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 1013
AccI GTMKAC 2 cut(s) 174, 1215
AccIII TCCGGA 2 cut(s) 574, 919
AciI CCGC 3 cut(s) 587, 963, 1170
AclWI GGATC 2 cut(s) 209, 643
AcsI RAATTY 4 cut(s) 245, 680, 731, 896
AfaI GTAC 3 cut(s) 362, 857, 1376
AhlI ACTAGT 1 cut(s) 656
AjnI CCWGG 1 cut(s) 430
AjuI GAANNNNNNNTTGG 2 cut(s) 749, 781
AluBI AGCT 8 cut(s) 94, 274, 389, 518, 568, 881, 1094, 1322
AluI AGCT 8 cut(s) 94, 274, 389, 518, 568, 881, 1094, 1322
Alw26I GTCTC 2 cut(s) 329, 869
AlwI GGATC 2 cut(s) 209, 643
Aor13HI TCCGGA 2 cut(s) 574, 919
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 6 cut(s) 386, 398, 518, 568, 878, 1087
ApoI RAATTY 4 cut(s) 245, 680, 731, 896
ArsI GACNNNNNNTTYG 6 cut(s) 888, 920, 1137, 1169, 1188, 1220
Asp700I GAANNNNTTC 1 cut(s) 606
AspLEI GCGC 1 cut(s) 1121
AsuC2I CCSGG 1 cut(s) 531
AsuHPI GGTGA 3 cut(s) 446, 898, 1169
AsuII TTCGAA 2 cut(s) 684, 1194
AxyI CCTNAGG 1 cut(s) 510
BaeGI GKGCMC 1 cut(s) 1016
BaeI ACNNNNGTAYC 2 cut(s) 847, 880
BanI GGYRCC 1 cut(s) 1013
BanII GRGCYC 1 cut(s) 16
BbvI GCAGC 6 cut(s) 398, 410, 505, 555, 890, 1074
BccI CCATC 3 cut(s) 21, 1070, 1178
BceAI ACGGC 2 cut(s) 446, 1357
BciT130I CCWGG 1 cut(s) 432
BciVI GTATCC 1 cut(s) 386
BclI TGATCA 3 cut(s) 366, 693, 1005
BcnI CCSGG 1 cut(s) 531
BcoDI GTCTC 2 cut(s) 329, 869
BcuI ACTAGT 1 cut(s) 656
BfaI CTAG 1 cut(s) 657
BfmI CTRYAG 1 cut(s) 1020
BfuI GTATCC 1 cut(s) 386
BglII AGATCT 1 cut(s) 634
BisI GCNGC 7 cut(s) 387, 399, 519, 569, 879, 963, 1088
BlsI GCNGC 7 cut(s) 388, 400, 520, 570, 880, 964, 1089
Bme1390I CCNGG 2 cut(s) 432, 531
BmiI GGNNCC 4 cut(s) 536, 709, 924, 1015
BmrFI CCNGG 2 cut(s) 432, 531
BmrI ACTGGG 1 cut(s) 675
BmsI GCATC 2 cut(s) 778, 1024
BmuI ACTGGG 1 cut(s) 675
Bpu14I TTCGAA 2 cut(s) 684, 1194
BpuEI CTTGAG 2 cut(s) 793, 1055
BpuMI CCSGG 1 cut(s) 531
BsaAI YACGTR 1 cut(s) 364
BsaI GGTCTC 1 cut(s) 869
BsaJI CCNNGG 5 cut(s) 530, 909, 926, 938, 1221
BsaWI WCCGGW 2 cut(s) 574, 919
BsaXI ACNNNNNCTCC 2 cut(s) 1367, 1397
Bse1I ACTGG 3 cut(s) 342, 670, 709
Bse21I CCTNAGG 1 cut(s) 510
Bse3DI GCAATG 2 cut(s) 696, 1230
BseAI TCCGGA 2 cut(s) 574, 919
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 5 cut(s) 530, 909, 926, 938, 1221
BseGI GGATG 2 cut(s) 172, 1081
BseMI GCAATG 2 cut(s) 696, 1230
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 3 cut(s) 342, 670, 709
BseRI GAGGAG 3 cut(s) 573, 864, 1008
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 6 cut(s) 398, 410, 505, 555, 890, 1074
BshFI GGCC 1 cut(s) 134
BshNI GGYRCC 1 cut(s) 1013
BsiSI CCGG 4 cut(s) 482, 530, 575, 920
BslFI GGGAC 2 cut(s) 1190, 1241
BsmAI GTCTC 2 cut(s) 329, 869
BsmFI GGGAC 2 cut(s) 1190, 1241
BsnI GGCC 1 cut(s) 134
Bso31I GGTCTC 1 cut(s) 869
Bsp119I TTCGAA 2 cut(s) 684, 1194
Bsp1286I GDGCHC 2 cut(s) 16, 1016
Bsp13I TCCGGA 2 cut(s) 574, 919
Bsp143I GATC 7 cut(s) 201, 366, 444, 634, 648, 693, 1005
Bsp19I CCATGG 1 cut(s) 909
BspACI CCGC 3 cut(s) 587, 963, 1170
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 502
BspEI TCCGGA 2 cut(s) 574, 919
BspLI GGNNCC 4 cut(s) 536, 709, 924, 1015
BspMAI CTGCAG 1 cut(s) 1024
BspPI GGATC 2 cut(s) 209, 643
BspQI GCTCTTC 1 cut(s) 84
BspT104I TTCGAA 2 cut(s) 684, 1194
BspT107I GGYRCC 1 cut(s) 1013
BspTNI GGTCTC 1 cut(s) 869
BsrDI GCAATG 2 cut(s) 696, 1230
BsrI ACTGG 3 cut(s) 342, 670, 709
BssECI CCNNGG 5 cut(s) 530, 909, 926, 938, 1221
BssMI GATC 7 cut(s) 201, 366, 444, 634, 648, 693, 1005
BssNAI GTATAC 1 cut(s) 1216
BssT1I CCWWGG 1 cut(s) 909
Bst1107I GTATAC 1 cut(s) 1216
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 1 cut(s) 927
Bst6I CTCTTC 4 cut(s) 84, 1155, 1264, 1350
BstAPI GCANNNNNTGC 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 364
BstBI TTCGAA 2 cut(s) 684, 1194
BstC8I GCNNGC 4 cut(s) 935, 1092, 1311, 1333
BstDEI CTNAG 4 cut(s) 74, 488, 510, 611
BstDSI CCRYGG 3 cut(s) 909, 926, 1221
BstF5I GGATG 2 cut(s) 172, 1081
BstHHI GCGC 1 cut(s) 1121
BstKTI GATC 7 cut(s) 204, 369, 447, 637, 651, 696, 1008
BstMAI GTCTC 2 cut(s) 329, 869
BstMBI GATC 7 cut(s) 201, 366, 444, 634, 648, 693, 1005
BstMWI GCNNNNNNNGC 6 cut(s) 266, 395, 815, 1310, 1319, 1328
BstNI CCWGG 1 cut(s) 432
BstNSI RCATGY 3 cut(s) 937, 1283, 1313
BstSCI CCNGG 2 cut(s) 430, 529
BstSFI CTRYAG 1 cut(s) 1020
BstSLI GKGCMC 1 cut(s) 1016
BstV1I GCAGC 6 cut(s) 398, 410, 505, 555, 890, 1074
BstX2I RGATCY 2 cut(s) 634, 648
BstXI CCANNNNNNTGG 1 cut(s) 1184
BstYI RGATCY 2 cut(s) 634, 648
BstZ17I GTATAC 1 cut(s) 1216
Bsu36I CCTNAGG 1 cut(s) 510
BsuI GTATCC 1 cut(s) 386
BsuRI GGCC 1 cut(s) 134
BtgI CCRYGG 3 cut(s) 909, 926, 1221
BtgZI GCGATG 1 cut(s) 801
BtsCI GGATG 2 cut(s) 172, 1081
BtsI GCAGTG 2 cut(s) 1017, 1068
BtsIMutI CAGTG 2 cut(s) 1017, 1068
Cac8I GCNNGC 4 cut(s) 935, 1092, 1311, 1333
CfoI GCGC 1 cut(s) 1121
CsiI ACCWGGT 1 cut(s) 430
Csp6I GTAC 3 cut(s) 361, 856, 1375
CviAII CATG 8 cut(s) 539, 910, 934, 1264, 1280, 1289, 1310, 1388
CviQI GTAC 3 cut(s) 361, 856, 1375
DdeI CTNAG 4 cut(s) 74, 488, 510, 611
DpnI GATC 7 cut(s) 203, 368, 446, 636, 650, 695, 1007
DpnII GATC 7 cut(s) 201, 366, 444, 634, 648, 693, 1005
Eam1104I CTCTTC 4 cut(s) 84, 1155, 1264, 1350
EarI CTCTTC 4 cut(s) 84, 1155, 1264, 1350
EciI GGCGGA 1 cut(s) 1185
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 1 cut(s) 16
Eco31I GGTCTC 1 cut(s) 869
Eco81I CCTNAGG 1 cut(s) 510
EcoRI GAATTC 1 cut(s) 680
EcoRII CCWGG 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 909
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 909
FaeI CATG 8 cut(s) 542, 913, 937, 1267, 1283, 1292, 1313, 1391
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FaqI GGGAC 2 cut(s) 1190, 1241
FatI CATG 8 cut(s) 538, 909, 933, 1263, 1279, 1288, 1309, 1387
FauNDI CATATG 1 cut(s) 8
FbaI TGATCA 3 cut(s) 366, 693, 1005
FblI GTMKAC 2 cut(s) 174, 1215
Fnu4HI GCNGC 7 cut(s) 387, 399, 519, 569, 879, 963, 1088
FokI GGATG 2 cut(s) 179, 1088
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 7 cut(s) 387, 399, 519, 569, 879, 963, 1088
FspBI CTAG 1 cut(s) 657
GlaI GCGC 1 cut(s) 1120
GluI GCNGC 7 cut(s) 387, 399, 519, 569, 879, 963, 1088
HaeIII GGCC 1 cut(s) 134
HapII CCGG 4 cut(s) 482, 530, 575, 920
HhaI GCGC 1 cut(s) 1121
Hin1II CATG 8 cut(s) 542, 913, 937, 1267, 1283, 1292, 1313, 1391
Hin6I GCGC 1 cut(s) 1119
HinP1I GCGC 1 cut(s) 1119
HincII GTYRAC 2 cut(s) 726, 1285
HindII GTYRAC 2 cut(s) 726, 1285
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 3 cut(s) 137, 888, 1173
HpaII CCGG 4 cut(s) 482, 530, 575, 920
HphI GGTGA 3 cut(s) 446, 898, 1169
Hpy166II GTNNAC 5 cut(s) 155, 175, 726, 1216, 1285
Hpy188I TCNGA 2 cut(s) 18, 875
Hpy188III TCNNGA 7 cut(s) 253, 509, 575, 711, 866, 920, 1404
Hpy8I GTNNAC 5 cut(s) 155, 175, 726, 1216, 1285
HpyAV CCTTC 2 cut(s) 155, 1334
HpyCH4III ACNGT 1 cut(s) 927
HpyCH4IV ACGT 2 cut(s) 363, 478
HpyF10VI GCNNNNNNNGC 6 cut(s) 266, 395, 815, 1310, 1319, 1328
HpyF3I CTNAG 4 cut(s) 74, 488, 510, 611
HpySE526I ACGT 2 cut(s) 363, 478
Hsp92II CATG 8 cut(s) 542, 913, 937, 1267, 1283, 1292, 1313, 1391
HspAI GCGC 1 cut(s) 1119
Kpn2I TCCGGA 2 cut(s) 574, 919
Ksp22I TGATCA 3 cut(s) 366, 693, 1005
Kzo9I GATC 7 cut(s) 201, 366, 444, 634, 648, 693, 1005
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 2 cut(s) 515, 875
Lsp1109I GCAGC 6 cut(s) 398, 410, 505, 555, 890, 1074
LweI GCATC 2 cut(s) 778, 1024
MabI ACCWGGT 1 cut(s) 430
MaeI CTAG 1 cut(s) 657
MaeII ACGT 2 cut(s) 363, 478
MaeIII GTNAC 3 cut(s) 55, 904, 977
MalI GATC 7 cut(s) 203, 368, 446, 636, 650, 695, 1007
MboI GATC 7 cut(s) 201, 366, 444, 634, 648, 693, 1005
MboII GAAGA 7 cut(s) 101, 122, 314, 629, 1172, 1281, 1367
MflI RGATCY 2 cut(s) 634, 648
MhlI GDGCHC 2 cut(s) 16, 1016
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 4 cut(s) 66, 529, 826, 853
MroI TCCGGA 2 cut(s) 574, 919
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 2 cut(s) 32, 357
MslI CAYNNNNRTG 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 4 cut(s) 482, 530, 575, 920
MspR9I CCNGG 2 cut(s) 432, 531
MvaI CCWGG 1 cut(s) 432
MwoI GCNNNNNNNGC 6 cut(s) 266, 395, 815, 1310, 1319, 1328
NciI CCSGG 1 cut(s) 531
NcoI CCATGG 1 cut(s) 909
NdeI CATATG 1 cut(s) 8
NdeII GATC 7 cut(s) 201, 366, 444, 634, 648, 693, 1005
NlaIII CATG 8 cut(s) 542, 913, 937, 1267, 1283, 1292, 1313, 1391
NlaIV GGNNCC 4 cut(s) 536, 709, 924, 1015
NmeAIII GCCGAG 4 cut(s) 160, 213, 919, 1330
NmuCI GTSAC 1 cut(s) 904
NspI RCATGY 3 cut(s) 937, 1283, 1313
NspV TTCGAA 2 cut(s) 684, 1194
PaeI GCATGC 2 cut(s) 937, 1313
PciSI GCTCTTC 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 2 cut(s) 888, 1173
PkrI GCNGC 7 cut(s) 388, 400, 520, 570, 880, 964, 1089
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
Ppu21I YACGTR 1 cut(s) 364
PsiI TTATAA 1 cut(s) 719
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 4 cut(s) 536, 709, 924, 1015
PstI CTGCAG 1 cut(s) 1024
PsuI RGATCY 2 cut(s) 634, 648
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 3 cut(s) 362, 857, 1376
RsaNI GTAC 3 cut(s) 361, 856, 1375
RseI CAYNNNNRTG 1 cut(s) 543
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 32, 357
SatI GCNGC 7 cut(s) 387, 399, 519, 569, 879, 963, 1088
Sau3AI GATC 7 cut(s) 201, 366, 444, 634, 648, 693, 1005
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 432, 531
SduI GDGCHC 2 cut(s) 16, 1016
SexAI ACCWGGT 1 cut(s) 430
SfaNI GCATC 2 cut(s) 778, 1024
SfcI CTRYAG 1 cut(s) 1020
SfuI TTCGAA 2 cut(s) 684, 1194
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 2 cut(s) 772, 1070
SmoI CTYRAG 2 cut(s) 772, 1070
SpeI ACTAGT 1 cut(s) 656
SphI GCATGC 2 cut(s) 937, 1313
SsiI CCGC 3 cut(s) 587, 963, 1170
SspMI CTAG 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 430, 529
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 1 cut(s) 927
TaiI ACGT 2 cut(s) 366, 481
TaqI TCGA 8 cut(s) 120, 254, 312, 627, 684, 867, 1032, 1194
TauI GCSGC 1 cut(s) 965
TfiI GAWTC 2 cut(s) 888, 1173
Tru1I TTAA 2 cut(s) 32, 357
Tru9I TTAA 2 cut(s) 32, 357
TscAI CASTG 2 cut(s) 1024, 1068
TseFI GTSAC 1 cut(s) 904
TseI GCWGC 6 cut(s) 386, 398, 518, 568, 878, 1087
Tsp45I GTSAC 1 cut(s) 904
TspDTI ATGAA 5 cut(s) 80, 183, 489, 629, 1262
TspGWI ACGGA 4 cut(s) 256, 843, 1062, 1210
TspRI CASTG 2 cut(s) 1024, 1068
XapI RAATTY 4 cut(s) 245, 680, 731, 896
XceI RCATGY 3 cut(s) 937, 1283, 1313
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XmiI GTMKAC 2 cut(s) 174, 1215
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.