Rw5G022280

Monodehydroascorbate reductase, seedling isozyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013840
Physical Location & Seq
Reverse (-)
30560854 .. 30562520
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022280.1

Sequence Viewer

Length: 1638 bp
ATGGCATTGTTGAAGCCAATTACTGAAAGAATACTAGTCATATGGGCTCTGATTACTAGCTGGTTGAAGTATCAAAGAAACCGTGAAGGAAATATGGAACTTCTGAACAGATTGTTAGAAGAGCTCGATAGCCAAACTGCAGATATAGAGCAAAAGCTAGACTCTGAGCCTCCTCATCCAATCAAAAGACAAGAAGATGAAGTCTATGAGTGGTTGGAAAATGTGGAAAGGATCAAAACTGAAGTGCAAACCATTTACCAAATCGCTGAAAGGAACTATCTCTCACCGGAACATTTCGGGAAGCTTTTGCTCCAGGAAGGAAAATTGTACACTGGGATTTCCGAGGAGAAGAGTTTCAAGTACTTGATCATTGGTGGTGGAGTTGCAGCTGGATATGCAGCCAGGGAGTTTGTCAAACAAGGGCTCAAGCCAGGTGAGTTAGCAATCATATCAAAAGAAGCAGTGGCGCCTTATGAACGTCCTGCGCTTAGTAAGGCCTACCTGTTTCCTGAGGGAGCTGTAAGACTTCCAGGGTTCAATGTCTGCGTTGGAAGTAGAGGTGAGAGGCTGCTACCTGAGTGGTATGTGGAGAAAGGTATTGAATTAATTCTTAGTACAGAAATTATCAAAGCAGATCTTGATTCGAAGATCCTCACCAGTGCAGCTGGGATGAAATATAGTTATGATATTTTGATAATTGCAACTGGTTCCAGAGCACTAAGGTTGACAGAGTTTGGTGTACACGGAGCTGATGCAAAGAACATTTTCTACTTGAGAGAAATCAGTGATGCTGATAAGCTTGTAGAAGAAATTAGAGCCAAGAAAAAGGGAAAGGTAGTAGTTGTTGGAGGAGGATACATTGGTCTTGAAGTTGGAGCTGCTATGAGAATCAACCAGTTTGATGTTACCATAGTTTATCGCGAACAATTGTTCATGGCACGGCTTTTCACTCAAGAGATAGCTGCTTTCTATGAGGCTTACTATGAAAACAAAGGAATCAAACTCATCAAAGGTACAGCTGCTGTTGCCTTTGATTCTGGTGCGAATGGAGAGGTAAAAGCAGTCAAACTCAAGGATGGTAGGGTGCTTGAAGCTGACATTGTTGTTGCTGGTGTTGGTGCAAAACCTCTCATTGAGTTATTTGAAGGGCAGCTTGCTAAAGCTAAAGATGGAATCAAAACTGATGGATTCTTTGAAACAGATGATCCCGACGTATATGCAGTAGGCGATGTGGCTACTTTCCCTATAAAGTTGTACAATGACATGAGAAGAGTTGAGCATGTTGACCATGCTAGGAAATCAGCAGAGCAAGCGGTAAAAGTTATCAAGGCAAGTGAAGAAAATAGATCTATTCCAGAGTATGATTGTCTTCCATACTTCTATTCGCGCGTCTTCAATCTTTCGTGGTCGTTTTATGGAGACAATGTGGGCAATCCTGTGCTATTTGGAGACAACAATCCGGCATCAGCGAAACCAAAGTTTGGAACAGCATGGATCAAGAATGGGAAGATTGTGGGAGTTTTCTTAGAGGGAGGGACAGCTGAAGAAAACAAGGCTATTGCCCAAATTGCAAGGGTCCAACCGCCAGCTGATAAGTATTTGGATCAGCTGAGAAAGGGTGATTATTTCTTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Pyr_redox_2

Protein Analysis

545

Amino Acids

60.87

Weight (kDa)

5.65

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 120 - 435 4.1e-56 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 278 - 360 2.4e-15 Pyridine nucleotide-disulphide oxidoreductase
MDHAR3-like_C PF21791 457 - 539 2.9e-34 Monodehydroascorbate reductase 3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013840)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09940 AT3G09940 AT5G03630
fragaria_vesca FvH4_3g20880
malus_domestica MD11G1237900.v1.1
prunus_persica Prupe.4G189300_v2.0.a1
pyrus_communis pycom03g16900 pycom11g20930
rosa_chinensis RchiOBHm_Chr5g0035251
rosa_laevigata RLG00000033613
rosa_multiflora Rmu_co8294091.1_g000001 Rmu_sc0007948.1_g000003
rosa_rugosa Rorug05G0151000
rosa_samantha Rh5BG243400 Rh5CG275100 Rh5DG252600
rosa_wichuraiana Rw5G022280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 466
AccB7I CCANNNNNTGG 1 cut(s) 1481
AccII CGCG 3 cut(s) 921, 1387, 1389
AciI CCGC 2 cut(s) 1313, 1583
AclWI GGATC 5 cut(s) 239, 643, 1199, 1502, 1611
AcuI CTGAAG 2 cut(s) 261, 1563
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 6 cut(s) 329, 362, 616, 741, 1015, 1256
AfiI CCNNNNNNNGG 1 cut(s) 1481
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 4 cut(s) 312, 401, 430, 529
Alw21I GWGCWC 2 cut(s) 126, 718
Alw26I GTCTC 2 cut(s) 1413, 1443
AlwI GGATC 5 cut(s) 239, 643, 1199, 1502, 1611
AlwNI CAGNNNCTG 1 cut(s) 1022
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 8 cut(s) 386, 398, 568, 662, 878, 962, 1019, 1150
AseI ATTAAT 1 cut(s) 605
Asp700I GAANNNNTTC 3 cut(s) 353, 606, 764
AspLEI GCGC 3 cut(s) 469, 487, 1389
AspS9I GGNCC 1 cut(s) 1576
AsuHPI GGTGA 5 cut(s) 276, 446, 572, 646, 1631
AsuII TTCGAA 1 cut(s) 644
AvaII GGWCC 1 cut(s) 1576
AxyI CCTNAGG 1 cut(s) 510
BaeI ACNNNNGTAYC 2 cut(s) 847, 880
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 3 cut(s) 49, 126, 426
BbsI GAAGAC 2 cut(s) 1361, 1384
Bbv12I GWGCWC 2 cut(s) 126, 718
BbvI GCAGC 8 cut(s) 398, 410, 555, 674, 865, 949, 1006, 1162
BccI CCATC 3 cut(s) 1070, 1163, 1178
BceAI ACGGC 1 cut(s) 956
BcgI CGANNNNNNTGC 2 cut(s) 1199, 1233
BciT130I CCWGG 4 cut(s) 314, 403, 432, 531
BciVI GTATCC 1 cut(s) 848
BclI TGATCA 1 cut(s) 366
BcoDI GTCTC 2 cut(s) 1413, 1443
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 4 cut(s) 35, 57, 158, 1293
BfmI CTRYAG 1 cut(s) 138
BfoI RGCGCY 1 cut(s) 470
BfuI GTATCC 1 cut(s) 848
BglII AGATCT 2 cut(s) 634, 1346
BisI GCNGC 8 cut(s) 387, 399, 569, 663, 879, 963, 1020, 1151
BlsI GCNGC 8 cut(s) 388, 400, 570, 664, 880, 964, 1021, 1152
BmcAI AGTACT 1 cut(s) 362
Bme1390I CCNGG 4 cut(s) 314, 403, 432, 531
Bme18I GGWCC 1 cut(s) 1576
BmgT120I GGNCC 1 cut(s) 1576
BmiI GGNNCC 3 cut(s) 468, 709, 1577
BmrFI CCNGG 4 cut(s) 314, 403, 432, 531
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 3 cut(s) 742, 778, 1472
BmuI ACTGGG 1 cut(s) 342
BpiI GAAGAC 2 cut(s) 1361, 1384
BpmI CTGGAG 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 644
BpuEI CTTGAG 4 cut(s) 410, 793, 936, 1055
BsaHI GRCGYC 1 cut(s) 467
BsaJI CCNNGG 3 cut(s) 342, 402, 530
BsaWI WCCGGW 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 1410, 1440
Bsc4I CCNNNNNNNGG 1 cut(s) 1481
Bse1I ACTGG 4 cut(s) 337, 657, 709, 895
Bse21I CCTNAGG 1 cut(s) 510
BseBI CCWGG 4 cut(s) 314, 403, 432, 531
BseDI CCNNGG 3 cut(s) 342, 402, 530
BseGI GGATG 3 cut(s) 175, 675, 1081
BseLI CCNNNNNNNGG 1 cut(s) 1481
BseMII CTCAG 4 cut(s) 156, 501, 567, 1601
BseNI ACTGG 4 cut(s) 337, 657, 709, 895
BseRI GAGGAG 3 cut(s) 162, 359, 864
BseXI GCAGC 8 cut(s) 398, 410, 555, 674, 865, 949, 1006, 1162
BseYI CCCAGC 1 cut(s) 665
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 3 cut(s) 921, 1387, 1389
BshFI GGCC 1 cut(s) 497
BshNI GGYRCC 1 cut(s) 466
BsiHKAI GWGCWC 2 cut(s) 126, 718
BsiSI CCGG 2 cut(s) 287, 1460
BslFI GGGAC 1 cut(s) 1549
BslI CCNNNNNNNGG 1 cut(s) 1481
BsmAI GTCTC 2 cut(s) 1413, 1443
BsmFI GGGAC 1 cut(s) 1549
BsnI GGCC 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 644
Bsp1286I GDGCHC 4 cut(s) 49, 126, 426, 718
Bsp1407I TGTACA 3 cut(s) 327, 739, 1254
Bsp143I GATC 8 cut(s) 231, 366, 634, 648, 1204, 1346, 1494, 1603
Bsp68I TCGCGA 1 cut(s) 921
BspACI CCGC 2 cut(s) 1313, 1583
BspANI GGCC 1 cut(s) 497
BspCNI CTCAG 4 cut(s) 157, 502, 568, 1602
BspFNI CGCG 3 cut(s) 921, 1387, 1389
BspLI GGNNCC 3 cut(s) 468, 709, 1577
BspMAI CTGCAG 1 cut(s) 142
BspPI GGATC 5 cut(s) 239, 643, 1199, 1502, 1611
BspQI GCTCTTC 1 cut(s) 114
BspT104I TTCGAA 1 cut(s) 644
BspT107I GGYRCC 1 cut(s) 466
BsrGI TGTACA 3 cut(s) 327, 739, 1254
BsrI ACTGG 4 cut(s) 337, 657, 709, 895
BssECI CCNNGG 3 cut(s) 342, 402, 530
BssMI GATC 8 cut(s) 231, 366, 634, 648, 1204, 1346, 1494, 1603
BssNI GRCGYC 1 cut(s) 467
Bst2UI CCWGG 4 cut(s) 314, 403, 432, 531
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 3 cut(s) 114, 344, 1264
BstACI GRCGYC 1 cut(s) 467
BstAUI TGTACA 3 cut(s) 327, 739, 1254
BstBI TTCGAA 1 cut(s) 644
BstC8I GCNNGC 3 cut(s) 1155, 1311, 1587
BstDEI CTNAG 8 cut(s) 165, 488, 510, 576, 611, 719, 1525, 1610
BstF5I GGATG 3 cut(s) 175, 675, 1081
BstFNI CGCG 3 cut(s) 921, 1387, 1389
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 3 cut(s) 469, 487, 1389
BstKTI GATC 8 cut(s) 234, 369, 637, 651, 1207, 1349, 1497, 1606
BstMAI GTCTC 2 cut(s) 1413, 1443
BstMBI GATC 8 cut(s) 231, 366, 634, 648, 1204, 1346, 1494, 1603
BstMWI GCNNNNNNNGC 4 cut(s) 395, 1025, 1310, 1568
BstNI CCWGG 4 cut(s) 314, 403, 432, 531
BstNSI RCATGY 1 cut(s) 1283
BstSCI CCNGG 4 cut(s) 312, 401, 430, 529
BstSFI CTRYAG 1 cut(s) 138
BstUI CGCG 3 cut(s) 921, 1387, 1389
BstV1I GCAGC 8 cut(s) 398, 410, 555, 674, 865, 949, 1006, 1162
BstV2I GAAGAC 2 cut(s) 1361, 1384
BstX2I RGATCY 3 cut(s) 634, 648, 1346
BstYI RGATCY 3 cut(s) 634, 648, 1346
Bsu36I CCTNAGG 1 cut(s) 510
BsuI GTATCC 1 cut(s) 848
BsuRI GGCC 1 cut(s) 497
BtgZI GCGATG 1 cut(s) 1242
BtsCI GGATG 3 cut(s) 175, 675, 1081
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 4 cut(s) 330, 468, 664, 790
BtuMI TCGCGA 1 cut(s) 921
Cac8I GCNNGC 3 cut(s) 1155, 1311, 1587
CaiI CAGNNNCTG 1 cut(s) 1022
CfoI GCGC 3 cut(s) 469, 487, 1389
Cfr13I GGNCC 1 cut(s) 1576
CseI GACGC 1 cut(s) 1378
Csp6I GTAC 6 cut(s) 328, 361, 615, 740, 1014, 1255
CviAII CATG 5 cut(s) 934, 1264, 1280, 1289, 1491
CviQI GTAC 6 cut(s) 328, 361, 615, 740, 1014, 1255
DdeI CTNAG 8 cut(s) 165, 488, 510, 576, 611, 719, 1525, 1610
DinI GGCGCC 1 cut(s) 468
DpnI GATC 8 cut(s) 233, 368, 636, 650, 1206, 1348, 1496, 1605
DpnII GATC 8 cut(s) 231, 366, 634, 648, 1204, 1346, 1494, 1603
Eam1104I CTCTTC 3 cut(s) 114, 344, 1264
EarI CTCTTC 3 cut(s) 114, 344, 1264
Ecl136II GAGCTC 1 cut(s) 124
Eco147I AGGCCT 1 cut(s) 497
Eco24I GRGCYC 3 cut(s) 49, 126, 426
Eco47I GGWCC 1 cut(s) 1576
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 2 cut(s) 261, 1563
Eco81I CCTNAGG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 124
EcoRII CCWGG 4 cut(s) 312, 401, 430, 529
EcoT38I GRGCYC 3 cut(s) 49, 126, 426
EgeI GGCGCC 1 cut(s) 468
EheI GGCGCC 1 cut(s) 468
FaeI CATG 5 cut(s) 937, 1267, 1283, 1292, 1494
FalI AAGNNNNNCTT 4 cut(s) 621, 653, 1137, 1169
FaqI GGGAC 1 cut(s) 1549
FatI CATG 5 cut(s) 933, 1263, 1279, 1288, 1490
FauNDI CATATG 1 cut(s) 41
FbaI TGATCA 1 cut(s) 366
Fnu4HI GCNGC 8 cut(s) 387, 399, 569, 663, 879, 963, 1020, 1151
FokI GGATG 3 cut(s) 162, 682, 1088
FriOI GRGCYC 3 cut(s) 49, 126, 426
Fsp4HI GCNGC 8 cut(s) 387, 399, 569, 663, 879, 963, 1020, 1151
FspBI CTAG 4 cut(s) 35, 57, 158, 1293
GlaI GCGC 3 cut(s) 468, 486, 1388
GluI GCNGC 8 cut(s) 387, 399, 569, 663, 879, 963, 1020, 1151
GsaI CCCAGC 1 cut(s) 669
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 470
HaeIII GGCC 1 cut(s) 497
HapII CCGG 2 cut(s) 287, 1460
HgaI GACGC 1 cut(s) 1378
HhaI GCGC 3 cut(s) 469, 487, 1389
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 5 cut(s) 937, 1267, 1283, 1292, 1494
Hin6I GCGC 3 cut(s) 467, 485, 1387
HinP1I GCGC 3 cut(s) 467, 485, 1387
HincII GTYRAC 2 cut(s) 726, 1285
HindII GTYRAC 2 cut(s) 726, 1285
HindIII AAGCTT 2 cut(s) 302, 797
HinfI GANTC 7 cut(s) 161, 641, 888, 996, 1034, 1173, 1188
HpaII CCGG 2 cut(s) 287, 1460
HphI GGTGA 5 cut(s) 276, 446, 572, 646, 1631
Hpy166II GTNNAC 5 cut(s) 330, 726, 740, 742, 1285
Hpy188I TCNGA 4 cut(s) 51, 105, 166, 343
Hpy8I GTNNAC 5 cut(s) 330, 726, 740, 742, 1285
Hpy99I CGWCG 1 cut(s) 1214
HpyAV CCTTC 3 cut(s) 80, 311, 1139
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 2 cut(s) 478, 1212
HpyF10VI GCNNNNNNNGC 4 cut(s) 395, 1025, 1310, 1568
HpyF3I CTNAG 8 cut(s) 165, 488, 510, 576, 611, 719, 1525, 1610
HpySE526I ACGT 2 cut(s) 478, 1212
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 5 cut(s) 937, 1267, 1283, 1292, 1494
HspAI GCGC 3 cut(s) 467, 485, 1387
KasI GGCGCC 1 cut(s) 466
Ksp22I TGATCA 1 cut(s) 366
Kzo9I GATC 8 cut(s) 231, 366, 634, 648, 1204, 1346, 1494, 1603
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 4 cut(s) 315, 515, 746, 875
Lsp1109I GCAGC 8 cut(s) 398, 410, 555, 674, 865, 949, 1006, 1162
LweI GCATC 3 cut(s) 742, 778, 1472
MaeI CTAG 4 cut(s) 35, 57, 158, 1293
MaeII ACGT 2 cut(s) 478, 1212
MaeIII GTNAC 1 cut(s) 904
MalI GATC 8 cut(s) 233, 368, 636, 650, 1206, 1348, 1496, 1605
MboI GATC 8 cut(s) 231, 366, 634, 648, 1204, 1346, 1494, 1603
MfeI CAATTG 1 cut(s) 926
MflI RGATCY 3 cut(s) 634, 648, 1346
MhlI GDGCHC 4 cut(s) 49, 126, 426, 718
MluCI AATT 9 cut(s) 18, 323, 602, 606, 621, 696, 810, 926, 1566
Mly113I GGCGCC 1 cut(s) 467
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 5 cut(s) 195, 529, 826, 853, 1603
MroXI GAANNNNTTC 3 cut(s) 353, 606, 764
MseI TTAA 1 cut(s) 605
MspA1I CMGCKG 6 cut(s) 389, 665, 1019, 1541, 1589, 1609
MspI CCGG 2 cut(s) 287, 1460
MspR9I CCNGG 4 cut(s) 314, 403, 432, 531
MunI CAATTG 1 cut(s) 926
MvaI CCWGG 4 cut(s) 314, 403, 432, 531
MvnI CGCG 3 cut(s) 921, 1387, 1389
MwoI GCNNNNNNNGC 4 cut(s) 395, 1025, 1310, 1568
NarI GGCGCC 1 cut(s) 467
NdeI CATATG 1 cut(s) 41
NdeII GATC 8 cut(s) 231, 366, 634, 648, 1204, 1346, 1494, 1603
NlaIII CATG 5 cut(s) 937, 1267, 1283, 1292, 1494
NlaIV GGNNCC 3 cut(s) 468, 709, 1577
NruI TCGCGA 1 cut(s) 921
NspI RCATGY 1 cut(s) 1283
NspV TTCGAA 1 cut(s) 644
PceI AGGCCT 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 114
PdmI GAANNNNTTC 3 cut(s) 353, 606, 764
PfeI GAWTC 6 cut(s) 641, 888, 996, 1034, 1173, 1188
PflMI CCANNNNNTGG 1 cut(s) 1481
PfoI TCCNGGA 1 cut(s) 312
PkrI GCNGC 8 cut(s) 388, 400, 570, 664, 880, 964, 1021, 1152
PleI GAGTC 1 cut(s) 155
PluTI GGCGCC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 155
PshBI ATTAAT 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 4 cut(s) 312, 401, 430, 529
PspFI CCCAGC 1 cut(s) 665
PspGI CCWGG 4 cut(s) 312, 401, 430, 529
PspN4I GGNNCC 3 cut(s) 468, 709, 1577
PspPI GGNCC 1 cut(s) 1576
PsrI GAACNNNNNNTAC 2 cut(s) 752, 784
PstI CTGCAG 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 3 cut(s) 634, 648, 1346
PvuII CAGCTG 6 cut(s) 389, 665, 1019, 1541, 1589, 1609
RruI TCGCGA 1 cut(s) 921
RsaI GTAC 6 cut(s) 329, 362, 616, 741, 1015, 1256
RsaNI GTAC 6 cut(s) 328, 361, 615, 740, 1014, 1255
SacI GAGCTC 1 cut(s) 126
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 605
SatI GCNGC 8 cut(s) 387, 399, 569, 663, 879, 963, 1020, 1151
Sau3AI GATC 8 cut(s) 231, 366, 634, 648, 1204, 1346, 1494, 1603
Sau96I GGNCC 1 cut(s) 1576
ScaI AGTACT 1 cut(s) 362
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 4 cut(s) 314, 403, 432, 531
SduI GDGCHC 4 cut(s) 49, 126, 426, 718
SfaNI GCATC 3 cut(s) 742, 778, 1472
SfcI CTRYAG 1 cut(s) 138
SfoI GGCGCC 1 cut(s) 468
SfuI TTCGAA 1 cut(s) 644
SinI GGWCC 1 cut(s) 1576
SmlI CTYRAG 4 cut(s) 425, 772, 951, 1070
SmoI CTYRAG 4 cut(s) 425, 772, 951, 1070
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 9 cut(s) 18, 323, 602, 606, 621, 696, 810, 926, 1566
SseBI AGGCCT 1 cut(s) 497
SsiI CCGC 2 cut(s) 1313, 1583
SspDI GGCGCC 1 cut(s) 466
SspMI CTAG 4 cut(s) 35, 57, 158, 1293
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 1 cut(s) 497
StyD4I CCNGG 4 cut(s) 312, 401, 430, 529
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 2 cut(s) 481, 1215
TaqI TCGA 2 cut(s) 126, 644
TasI AATT 9 cut(s) 18, 323, 602, 606, 621, 696, 810, 926, 1566
TatI WGTACW 5 cut(s) 327, 360, 614, 739, 1254
TfiI GAWTC 6 cut(s) 641, 888, 996, 1034, 1173, 1188
Tru1I TTAA 1 cut(s) 605
Tru9I TTAA 1 cut(s) 605
TscAI CASTG 4 cut(s) 337, 468, 664, 790
TseI GCWGC 8 cut(s) 386, 398, 568, 662, 878, 962, 1019, 1150
TspDTI ATGAA 5 cut(s) 213, 489, 686, 922, 999
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 4 cut(s) 337, 468, 664, 790
Van91I CCANNNNNTGG 1 cut(s) 1481
VpaK11BI GGWCC 1 cut(s) 1576
VspI ATTAAT 1 cut(s) 605
XceI RCATGY 1 cut(s) 1283
XmnI GAANNNNTTC 3 cut(s) 353, 606, 764
XspI CTAG 4 cut(s) 35, 57, 158, 1293
ZrmI AGTACT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.