MD12G1001600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007300
Physical Location & Seq
Reverse (-)
288155 .. 298327
10173 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1001600.v1.1.491

Sequence Viewer

Length: 405 bp
ATGGCCGATTCCAAACCTGAAACTAGTATGAGATGCCATCATTGTGCAGGTCCTCTTTCTATGAAGATGGAAACGAGCGAGTGGACTGTTGCACCTCTTATTAGGGATAGCTTTTCAATGATTGGCTCAGCTGTTGGTGGCACAGCAAGTGCTTTTTATGGATTCAACCATGTGATGCCTATAGTTCGGAGGTGGGTAAAAGGACCCATGTGGTTGCACTTTTTCATTGGTGCTCCGCCTGTGATTGTATTCTCTTCAGCTTGTGCAGGTTTAGCAGGCGGTGCCGTTCCAGCGTTGGCCCAACTTGCTTCTTCATCTTATCATGCAGTGATCTCATCGTCCTCGTTGCCGCCAACTTCTCAAGATGAAAAGATGCAGGAGTCCAGAACTTCCTCGATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.25

Weight (kDa)

7.8

Isoelectric Point (pI)

63.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 38, 257
AccB1I GGYRCC 1 cut(s) 281
AciI CCGC 3 cut(s) 236, 279, 350
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 240
AgsI TTSAA 2 cut(s) 117, 166
AhlI ACTAGT 1 cut(s) 23
AluBI AGCT 3 cut(s) 111, 131, 260
AluI AGCT 3 cut(s) 111, 131, 260
Alw21I GWGCWC 1 cut(s) 235
AoxI GGCC 2 cut(s) 3, 297
AspS9I GGNCC 3 cut(s) 50, 203, 298
AvaII GGWCC 2 cut(s) 50, 203
BanI GGYRCC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 235
BccI CCATC 2 cut(s) 45, 61
BceAI ACGGC 1 cut(s) 269
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 180
BfuAI ACCTGC 2 cut(s) 38, 257
BisI GCNGC 1 cut(s) 350
BlpI GCTNAGC 1 cut(s) 127
BlsI GCNGC 1 cut(s) 351
Bme18I GGWCC 2 cut(s) 50, 203
BmgT120I GGNCC 3 cut(s) 50, 203, 298
BmiI GGNNCC 2 cut(s) 205, 283
BmsI GCATC 3 cut(s) 23, 165, 363
Bpu1102I GCTNAGC 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 141
BsgI GTGCAG 2 cut(s) 66, 285
BshFI GGCC 2 cut(s) 5, 299
BshNI GGYRCC 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 235
BsnI GGCC 2 cut(s) 5, 299
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 1 cut(s) 330
Bsp1720I GCTNAGC 1 cut(s) 127
BspACI CCGC 3 cut(s) 236, 279, 350
BspANI GGCC 2 cut(s) 5, 299
BspCNI CTCAG 1 cut(s) 140
BspLI GGNNCC 2 cut(s) 205, 283
BspMI ACCTGC 2 cut(s) 38, 257
BspT107I GGYRCC 1 cut(s) 281
BssMI GATC 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 1 cut(s) 259
BstAPI GCANNNNNTGC 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 1 cut(s) 127
BstKTI GATC 1 cut(s) 333
BstMBI GATC 1 cut(s) 330
BstMWI GCNNNNNNNGC 4 cut(s) 272, 281, 290, 305
BstSFI CTRYAG 1 cut(s) 180
BsuRI GGCC 2 cut(s) 5, 299
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 333
BveI ACCTGC 2 cut(s) 38, 257
Cac8I GCNNGC 1 cut(s) 277
Cfr13I GGNCC 3 cut(s) 50, 203, 298
CviAII CATG 3 cut(s) 170, 208, 323
CviJI RGCY 6 cut(s) 5, 111, 126, 131, 260, 299
CviKI_1 RGCY 6 cut(s) 5, 111, 126, 131, 260, 299
DdeI CTNAG 1 cut(s) 127
DpnI GATC 1 cut(s) 332
DpnII GATC 1 cut(s) 330
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 259
EciI GGCGGA 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 50, 203
Eco57I CTGAAG 1 cut(s) 240
EcoO109I RGGNCCY 2 cut(s) 50, 203
FaeI CATG 3 cut(s) 173, 211, 326
FaiI YATR 7 cut(s) 29, 62, 159, 171, 182, 209, 324
FatI CATG 3 cut(s) 169, 207, 322
Fnu4HI GCNGC 1 cut(s) 350
Fsp4HI GCNGC 1 cut(s) 350
FspBI CTAG 1 cut(s) 24
GluI GCNGC 1 cut(s) 350
HaeIII GGCC 2 cut(s) 5, 299
Hin1II CATG 3 cut(s) 173, 211, 326
HinfI GANTC 4 cut(s) 8, 162, 380, 397
Hpy166II GTNNAC 1 cut(s) 84
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 2 cut(s) 362, 384
Hpy8I GTNNAC 1 cut(s) 84
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4V TGCA 6 cut(s) 47, 92, 217, 266, 326, 376
HpyF10VI GCNNNNNNNGC 4 cut(s) 272, 281, 290, 305
HpyF3I CTNAG 1 cut(s) 127
Hsp92II CATG 3 cut(s) 173, 211, 326
Kzo9I GATC 1 cut(s) 330
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 8 cut(s) 30, 33, 252, 252, 261, 303, 362, 397
LweI GCATC 3 cut(s) 23, 165, 363
MaeI CTAG 1 cut(s) 24
MalI GATC 1 cut(s) 332
MboI GATC 1 cut(s) 330
MboII GAAGA 3 cut(s) 76, 246, 303
MhlI GDGCHC 1 cut(s) 235
MlyI GAGTC 1 cut(s) 389
MnlI CCTC 5 cut(s) 63, 105, 183, 352, 403
MslI CAYNNNNRTG 1 cut(s) 42
MspA1I CMGCKG 1 cut(s) 131
MwoI GCNNNNNNNGC 4 cut(s) 272, 281, 290, 305
NdeII GATC 1 cut(s) 330
NlaIII CATG 3 cut(s) 173, 211, 326
NlaIV GGNNCC 2 cut(s) 205, 283
PfeI GAWTC 3 cut(s) 8, 162, 397
PkrI GCNGC 1 cut(s) 351
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PpuMI RGGWCCY 2 cut(s) 50, 203
Psp5II RGGWCCY 2 cut(s) 50, 203
PspN4I GGNNCC 2 cut(s) 205, 283
PspPI GGNCC 3 cut(s) 50, 203, 298
PspPPI RGGWCCY 2 cut(s) 50, 203
PvuII CAGCTG 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 42
SatI GCNGC 1 cut(s) 350
Sau3AI GATC 1 cut(s) 330
Sau96I GGNCC 3 cut(s) 50, 203, 298
SchI GAGTC 1 cut(s) 389
SduI GDGCHC 1 cut(s) 235
SetI ASST 8 cut(s) 19, 52, 97, 113, 133, 194, 262, 271
SfaNI GCATC 3 cut(s) 23, 165, 363
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 2 cut(s) 50, 203
SmiMI CAYNNNNRTG 1 cut(s) 42
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
SpeI ACTAGT 1 cut(s) 23
SsiI CCGC 3 cut(s) 236, 279, 350
SspMI CTAG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 88
TaqI TCGA 1 cut(s) 395
TauI GCSGC 1 cut(s) 352
TfiI GAWTC 3 cut(s) 8, 162, 397
TscAI CASTG 1 cut(s) 333
TspDTI ATGAA 4 cut(s) 77, 214, 303, 381
TspRI CASTG 1 cut(s) 333
VpaK11BI GGWCC 2 cut(s) 50, 203
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.