MD14G1001500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007300
Physical Location & Seq
Reverse (-)
280986 .. 286222
5237 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1001500.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCCGATTCCAAACCTGAAACTAGTACTGGCATGAGATGCCATCATTGTGCAGGTCCTCTTTCTAAGAAGATGGAAACGAGCGAGTGGACTGTTGCACCTCTTATTAGGGATAGCTTTTCCATGATTGGGTCGGCTGTTGGTGGCACAGCAAGTGCTTTTTATGGATTCAACCATGTGATGCCTATTGTTCGAAGATGGGTAAAAGGACCTATGTGGTTGCATTTTTTTTTTGGTGCTCCGCCTGTGATTGTATTCTCTTCAGCTTGTGCAGGTTTGGCAGGGGGTGCCGTTCCAGCGTTGGCCCAACTTGCTTCTTCATCTTATCGTGCAGTGGTCTCACCGTCCTCGTTGCCTCCAACTTGTCAAGATGAGAAGATGCACAAGTCCAGAACTTCCTCGATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.47

Weight (kDa)

9.3

Isoelectric Point (pI)

63.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 44, 263
AccB1I GGYRCC 1 cut(s) 287
AciI CCGC 1 cut(s) 242
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 28
AfiI CCNNNNNNNGG 1 cut(s) 129
AgsI TTSAA 1 cut(s) 172
AhlI ACTAGT 1 cut(s) 23
AluBI AGCT 2 cut(s) 117, 266
AluI AGCT 2 cut(s) 117, 266
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 1 cut(s) 343
AoxI GGCC 2 cut(s) 3, 303
AspS9I GGNCC 3 cut(s) 56, 209, 304
AsuHPI GGTGA 1 cut(s) 333
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 2 cut(s) 56, 209
BanI GGYRCC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 241
BccI CCATC 3 cut(s) 51, 67, 192
BceAI ACGGC 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 343
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 1 cut(s) 24
BfuAI ACCTGC 2 cut(s) 44, 263
BmcAI AGTACT 1 cut(s) 28
Bme18I GGWCC 2 cut(s) 56, 209
BmgT120I GGNCC 3 cut(s) 56, 209, 304
BmiI GGNNCC 1 cut(s) 289
BmsI GCATC 3 cut(s) 29, 171, 369
Bpu14I TTCGAA 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 343
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 34
BseLI CCNNNNNNNGG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 34
BsgI GTGCAG 3 cut(s) 72, 291, 351
BshFI GGCC 2 cut(s) 5, 305
BshNI GGYRCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 241
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 1 cut(s) 343
BsnI GGCC 2 cut(s) 5, 305
Bso31I GGTCTC 1 cut(s) 343
Bsp119I TTCGAA 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 241
BspACI CCGC 1 cut(s) 242
BspANI GGCC 2 cut(s) 5, 305
BspLI GGNNCC 1 cut(s) 289
BspMI ACCTGC 2 cut(s) 44, 263
BspT104I TTCGAA 1 cut(s) 193
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 343
BsrI ACTGG 1 cut(s) 34
Bst4CI ACNGT 2 cut(s) 94, 345
Bst6I CTCTTC 1 cut(s) 265
BstAPI GCANNNNNTGC 2 cut(s) 39, 287
BstBI TTCGAA 1 cut(s) 193
BstDEI CTNAG 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 343
BstMWI GCNNNNNNNGC 5 cut(s) 39, 278, 287, 296, 311
BsuRI GGCC 2 cut(s) 5, 305
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 339
BveI ACCTGC 2 cut(s) 44, 263
Cfr13I GGNCC 3 cut(s) 56, 209, 304
Csp6I GTAC 1 cut(s) 27
CviAII CATG 3 cut(s) 34, 124, 176
CviJI RGCY 5 cut(s) 5, 117, 137, 266, 305
CviKI_1 RGCY 5 cut(s) 5, 117, 137, 266, 305
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
EciI GGCGGA 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 343
Eco47I GGWCC 2 cut(s) 56, 209
Eco57I CTGAAG 1 cut(s) 246
EcoO109I RGGNCCY 2 cut(s) 56, 209
FaeI CATG 3 cut(s) 37, 127, 179
FaiI YATR 5 cut(s) 35, 125, 165, 177, 215
FatI CATG 3 cut(s) 33, 123, 175
FspBI CTAG 1 cut(s) 24
HaeIII GGCC 2 cut(s) 5, 305
Hin1II CATG 3 cut(s) 37, 127, 179
HinfI GANTC 3 cut(s) 8, 168, 403
HphI GGTGA 1 cut(s) 333
Hpy166II GTNNAC 1 cut(s) 90
Hpy188III TCNNGA 2 cut(s) 368, 390
Hpy8I GTNNAC 1 cut(s) 90
HpyCH4III ACNGT 2 cut(s) 94, 345
HpyCH4V TGCA 6 cut(s) 53, 98, 223, 272, 332, 382
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 278, 287, 296, 311
HpyF3I CTNAG 1 cut(s) 66
Hsp92II CATG 3 cut(s) 37, 127, 179
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 8 cut(s) 15, 30, 39, 258, 258, 267, 309, 403
LweI GCATC 3 cut(s) 29, 171, 369
MaeI CTAG 1 cut(s) 24
MboII GAAGA 5 cut(s) 82, 207, 252, 309, 388
MhlI GDGCHC 1 cut(s) 241
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 5 cut(s) 69, 111, 358, 366, 409
MslI CAYNNNNRTG 1 cut(s) 48
MwoI GCNNNNNNNGC 5 cut(s) 39, 278, 287, 296, 311
NlaIII CATG 3 cut(s) 37, 127, 179
NlaIV GGNNCC 1 cut(s) 289
NspV TTCGAA 1 cut(s) 193
PfeI GAWTC 3 cut(s) 8, 168, 403
PpuMI RGGWCCY 2 cut(s) 56, 209
Psp5II RGGWCCY 2 cut(s) 56, 209
PspN4I GGNNCC 1 cut(s) 289
PspPI GGNCC 3 cut(s) 56, 209, 304
PspPPI RGGWCCY 2 cut(s) 56, 209
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
RseI CAYNNNNRTG 1 cut(s) 48
Sau96I GGNCC 3 cut(s) 56, 209, 304
ScaI AGTACT 1 cut(s) 28
SduI GDGCHC 1 cut(s) 241
SetI ASST 7 cut(s) 19, 58, 103, 119, 214, 268, 277
SfaNI GCATC 3 cut(s) 29, 171, 369
SfuI TTCGAA 1 cut(s) 193
SinI GGWCC 2 cut(s) 56, 209
SmiMI CAYNNNNRTG 1 cut(s) 48
SpeI ACTAGT 1 cut(s) 23
SsiI CCGC 1 cut(s) 242
SspMI CTAG 1 cut(s) 24
TaaI ACNGT 2 cut(s) 94, 345
TaqI TCGA 2 cut(s) 193, 401
TatI WGTACW 1 cut(s) 26
TfiI GAWTC 3 cut(s) 8, 168, 403
TscAI CASTG 1 cut(s) 339
TspDTI ATGAA 1 cut(s) 309
TspRI CASTG 1 cut(s) 339
VpaK11BI GGWCC 2 cut(s) 56, 209
XspI CTAG 1 cut(s) 24
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.