MD12G1018600.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014762
Physical Location & Seq
Forward (+)
1779531 .. 1780445
915 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1018600.v1.1.491

Sequence Viewer

Length: 915 bp
ATGAGAAGCATAGCAACTTGTTATAGCGAGCATGCCATAAAGGTCTCAGATTCGTATTGCTCAGGCCCATCAAACCAAGCTTATGTGACCCCAAAATTAACCCCATCAATCCCAAACGAAGTGTCATGTTTATACAGAGCCAGGCTCTCAACCCAGAACCGGATTTTGATCACCCTCACCTGGTTCAACAAATTCACTGCCCAAGGACTCACGGTCACCGTTGGAGACAACAAATCTCAGCCGTCCAAATCCAATTCCGATTCCCACAAGTTGGAAAACCAAAAGGGCACCTTGGCATTCCAATCCTGCAATACCAAGATTGAAGTCTTCTGGGATTTGTCCACTGCCAATTATGATTCCGGACCGGAACCGGCAACCGGGTTTTTCGTCATGGTTCTAGCTGACTCGGAACTCTGCCTCCTCCTCGGCGACAAGGTCGAACAAGTTCTGGACTTGGAGAAGTACAAAACCATCATGTCACAGAAAAAATTCACATTGGTTTCTAGGAGTGAGCATTTTTCAGGCAGTGCTGTGTATGCAACAAAAGCTCAATTCTGTGACACTGGACTATCACATGACATCGTGATCAAGTGTGATCAACCCGAAGAACCGGGCTCGAAAAGCCCGGTTTTATCGGTCTGCATTGACAAGAAGAAGGTTTTTCAGGTGAAGAGGTTGAGGTGGAATTTCAGAGGAAACCAAACAATTTTTTTGGATGGTTTGCTGGTGGATTTGATGTGGGATATCCATGACTGGTTGTTCAATCCAAAATCTGGGGTAGCTGTCTTTATGTTCAGGACAAGGAGTGGATTGGATAGCAGGCTCTGGTTGGAAGACAAGAATTTAGAAAAGAAAGGGCAAGAAAGTGCTGATGAATTTTCTTTCTTGATCTGTGCGTGTAAGAGACCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

305

Amino Acids

34.26

Weight (kDa)

7.97

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 38 - 301 1.4e-80 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014762)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17950
malus_domestica MD12G1018600.v1.1 MD14G1016600.v1.1
prunus_persica Prupe.7G116700_v2.0.a1
pyrus_communis pycom12g01540 pycom14g01420
rosa_chinensis RchiOBHm_Chr3g0472211
rosa_laevigata RLG00000024097
rosa_multiflora Rmu_sc0002532.1_g000035
rosa_roxburghii Rroxscaffold_6G00409150
rosa_rugosa Rorug03G0122800
rosa_samantha Rh3AG175200 Rh3BG200200 Rh3CG191700 Rh3CG199000 Rh3DG174200
rosa_wichuraiana Rw3G016610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 287
AccB7I CCANNNNNTGG 2 cut(s) 271, 773
AccIII TCCGGA 1 cut(s) 359
AcsI RAATTY 5 cut(s) 191, 488, 685, 841, 875
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 5 cut(s) 159, 180, 271, 377, 773
AgsI TTSAA 3 cut(s) 187, 323, 763
AhdI GACNNNNNGTC 1 cut(s) 212
AjnI CCWGG 2 cut(s) 140, 179
AloI GAACNNNNNNTCC 2 cut(s) 402, 434
AluBI AGCT 4 cut(s) 80, 401, 548, 782
AluI AGCT 4 cut(s) 80, 401, 548, 782
Alw26I GTCTC 3 cut(s) 49, 219, 898
AlwNI CAGNNNCTG 1 cut(s) 825
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 1 cut(s) 64
ApoI RAATTY 5 cut(s) 191, 488, 685, 841, 875
ArsI GACNNNNNNTTYG 2 cut(s) 107, 139
Asp700I GAANNNNTTC 2 cut(s) 444, 488
AspS9I GGNCC 2 cut(s) 65, 362
AsuC2I CCSGG 3 cut(s) 379, 612, 626
AsuHPI GGTGA 4 cut(s) 163, 169, 208, 679
AvaII GGWCC 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 290
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 617
BbsI GAAGAC 2 cut(s) 319, 840
BccI CCATC 4 cut(s) 76, 112, 479, 710
BceAI ACGGC 1 cut(s) 226
BciT130I CCWGG 2 cut(s) 142, 181
BclI TGATCA 3 cut(s) 168, 585, 595
BcnI CCSGG 3 cut(s) 379, 612, 626
BcoDI GTCTC 3 cut(s) 49, 219, 898
BfaI CTAG 2 cut(s) 398, 504
Bme1390I CCNGG 5 cut(s) 142, 181, 379, 612, 626
Bme18I GGWCC 1 cut(s) 362
BmeRI GACNNNNNGTC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 65, 362
BmiI GGNNCC 2 cut(s) 289, 369
BmrFI CCNGG 5 cut(s) 142, 181, 379, 612, 626
BpiI GAAGAC 2 cut(s) 319, 840
BplI GAGNNNNNCTC 2 cut(s) 129, 161
Bpu10I CCTNAGC 1 cut(s) 61
BpuMI CCSGG 3 cut(s) 379, 612, 626
BsaBI GATNNNNATC 1 cut(s) 167
BsaI GGTCTC 2 cut(s) 49, 898
BsaJI CCNNGG 3 cut(s) 202, 291, 424
BsaWI WCCGGW 3 cut(s) 159, 359, 364
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 5 cut(s) 159, 180, 271, 377, 773
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 2 cut(s) 568, 758
Bse8I GATNNNNATC 1 cut(s) 167
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 2 cut(s) 142, 181
BseDI CCNNGG 3 cut(s) 202, 291, 424
BseGI GGATG 1 cut(s) 721
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 5 cut(s) 159, 180, 271, 377, 773
BseMII CTCAG 3 cut(s) 60, 75, 251
BseNI ACTGG 2 cut(s) 568, 758
BseRI GAGGAG 2 cut(s) 410, 413
BseSI GKGCMC 1 cut(s) 290
BshFI GGCC 1 cut(s) 66
BshNI GGYRCC 1 cut(s) 287
BsiSI CCGG 7 cut(s) 160, 360, 365, 371, 378, 611, 626
BslI CCNNNNNNNGG 5 cut(s) 159, 180, 271, 377, 773
BsmAI GTCTC 3 cut(s) 49, 219, 898
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 1 cut(s) 66
Bso31I GGTCTC 2 cut(s) 49, 898
Bsp1286I GDGCHC 2 cut(s) 290, 617
Bsp13I TCCGGA 1 cut(s) 359
Bsp143I GATC 4 cut(s) 168, 585, 595, 888
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 3 cut(s) 59, 74, 250
BspEI TCCGGA 1 cut(s) 359
BspLI GGNNCC 2 cut(s) 289, 369
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 2 cut(s) 49, 898
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 2 cut(s) 568, 758
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 3 cut(s) 202, 291, 424
BssMI GATC 4 cut(s) 168, 585, 595, 888
BssT1I CCWWGG 2 cut(s) 202, 291
Bst2UI CCWGG 2 cut(s) 142, 181
Bst4CI ACNGT 2 cut(s) 214, 220
Bst6I CTCTTC 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 29, 33, 821
BstDEI CTNAG 3 cut(s) 46, 61, 237
BstEII GGTNACC 1 cut(s) 214
BstF5I GGATG 1 cut(s) 721
BstKTI GATC 4 cut(s) 171, 588, 598, 891
BstMAI GTCTC 3 cut(s) 49, 219, 898
BstMBI GATC 4 cut(s) 168, 585, 595, 888
BstMWI GCNNNNNNNGC 3 cut(s) 536, 545, 621
BstNI CCWGG 2 cut(s) 142, 181
BstNSI RCATGY 1 cut(s) 35
BstPI GGTNACC 1 cut(s) 214
BstSCI CCNGG 5 cut(s) 140, 179, 377, 610, 624
BstSLI GKGCMC 1 cut(s) 290
BstV2I GAAGAC 2 cut(s) 319, 840
BsuRI GGCC 1 cut(s) 66
BtsCI GGATG 1 cut(s) 721
BtsI GCAGTG 3 cut(s) 195, 342, 532
BtsIMutI CAGTG 4 cut(s) 195, 342, 532, 561
Cac8I GCNNGC 3 cut(s) 29, 33, 821
CaiI CAGNNNCTG 1 cut(s) 825
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 2 cut(s) 65, 362
CpoI CGGWCCG 1 cut(s) 362
CsiI ACCWGGT 1 cut(s) 179
Csp6I GTAC 1 cut(s) 463
CspI CGGWCCG 1 cut(s) 362
CviAII CATG 6 cut(s) 32, 126, 391, 475, 575, 749
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 3 cut(s) 46, 61, 237
DpnI GATC 4 cut(s) 170, 587, 597, 890
DpnII GATC 4 cut(s) 168, 585, 595, 888
DriI GACNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 1 cut(s) 665
Eam1105I GACNNNNNGTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 665
Eco130I CCWWGG 2 cut(s) 202, 291
Eco24I GRGCYC 1 cut(s) 617
Eco31I GGTCTC 2 cut(s) 49, 898
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 362
Eco91I GGTNACC 1 cut(s) 214
EcoO65I GGTNACC 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 140, 179
EcoRV GATATC 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 202, 291
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 2 cut(s) 202, 291
FaeI CATG 6 cut(s) 35, 129, 394, 478, 578, 752
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FatI CATG 6 cut(s) 31, 125, 390, 474, 574, 748
FbaI TGATCA 3 cut(s) 168, 585, 595
FokI GGATG 1 cut(s) 728
FriOI GRGCYC 1 cut(s) 617
FspBI CTAG 2 cut(s) 398, 504
HaeIII GGCC 1 cut(s) 66
HapII CCGG 7 cut(s) 160, 360, 365, 371, 378, 611, 626
Hin1II CATG 6 cut(s) 35, 129, 394, 478, 578, 752
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 5 cut(s) 50, 207, 260, 356, 404
HpaII CCGG 7 cut(s) 160, 360, 365, 371, 378, 611, 626
HphI GGTGA 4 cut(s) 163, 169, 208, 679
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 4 cut(s) 49, 259, 409, 692
Hpy188III TCNNGA 5 cut(s) 360, 449, 583, 796, 886
Hpy8I GTNNAC 1 cut(s) 342
HpyAV CCTTC 1 cut(s) 649
HpyCH4III ACNGT 2 cut(s) 214, 220
HpyCH4V TGCA 3 cut(s) 309, 539, 642
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 545, 621
HpyF3I CTNAG 3 cut(s) 46, 61, 237
Hsp92II CATG 6 cut(s) 35, 129, 394, 478, 578, 752
Kpn2I TCCGGA 1 cut(s) 359
Ksp22I TGATCA 3 cut(s) 168, 585, 595
Kzo9I GATC 4 cut(s) 168, 585, 595, 888
MabI ACCWGGT 1 cut(s) 179
MaeI CTAG 2 cut(s) 398, 504
MaeIII GTNAC 4 cut(s) 85, 214, 477, 557
MalI GATC 4 cut(s) 170, 587, 597, 890
MboI GATC 4 cut(s) 168, 585, 595, 888
MboII GAAGA 5 cut(s) 319, 617, 664, 682, 845
MhlI GDGCHC 2 cut(s) 290, 617
MlyI GAGTC 2 cut(s) 201, 398
MmeI TCCRAC 3 cut(s) 202, 252, 810
MnlI CCTC 7 cut(s) 185, 428, 431, 434, 666, 672, 686
MroI TCCGGA 1 cut(s) 359
MroXI GAANNNNTTC 2 cut(s) 444, 488
MseI TTAA 1 cut(s) 98
MspI CCGG 7 cut(s) 160, 360, 365, 371, 378, 611, 626
MspR9I CCNGG 5 cut(s) 142, 181, 379, 612, 626
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 2 cut(s) 142, 181
MwoI GCNNNNNNNGC 3 cut(s) 536, 545, 621
NciI CCSGG 3 cut(s) 379, 612, 626
NdeII GATC 4 cut(s) 168, 585, 595, 888
NlaIII CATG 6 cut(s) 35, 129, 394, 478, 578, 752
NlaIV GGNNCC 2 cut(s) 289, 369
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 4 cut(s) 85, 214, 477, 557
NspI RCATGY 1 cut(s) 35
PaeI GCATGC 1 cut(s) 35
PctI GAATGC 1 cut(s) 296
PdmI GAANNNNTTC 2 cut(s) 444, 488
PfeI GAWTC 3 cut(s) 50, 260, 356
PflFI GACNNNGTC 1 cut(s) 434
PflMI CCANNNNNTGG 2 cut(s) 271, 773
PleI GAGTC 2 cut(s) 201, 398
PpsI GAGTC 2 cut(s) 201, 398
Psp6I CCWGG 2 cut(s) 140, 179
PspEI GGTNACC 1 cut(s) 214
PspGI CCWGG 2 cut(s) 140, 179
PspN4I GGNNCC 2 cut(s) 289, 369
PspPI GGNCC 2 cut(s) 65, 362
PstNI CAGNNNCTG 1 cut(s) 825
PsyI GACNNNGTC 1 cut(s) 434
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
Rsr2I CGGWCCG 1 cut(s) 362
RsrII CGGWCCG 1 cut(s) 362
SaqAI TTAA 1 cut(s) 98
Sau3AI GATC 4 cut(s) 168, 585, 595, 888
Sau96I GGNCC 2 cut(s) 65, 362
SchI GAGTC 2 cut(s) 201, 398
ScrFI CCNGG 5 cut(s) 142, 181, 379, 612, 626
SduI GDGCHC 2 cut(s) 290, 617
SexAI ACCWGGT 1 cut(s) 179
SinI GGWCC 1 cut(s) 362
SphI GCATGC 1 cut(s) 35
SspMI CTAG 2 cut(s) 398, 504
StyD4I CCNGG 5 cut(s) 140, 179, 377, 610, 624
StyI CCWWGG 2 cut(s) 202, 291
TaaI ACNGT 2 cut(s) 214, 220
TaqI TCGA 2 cut(s) 438, 617
TaqII GACCGA 1 cut(s) 625
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 3 cut(s) 50, 260, 356
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 4 cut(s) 202, 349, 532, 568
TseFI GTSAC 4 cut(s) 85, 214, 477, 557
Tsp45I GTSAC 4 cut(s) 85, 214, 477, 557
TspDTI ATGAA 1 cut(s) 888
TspRI CASTG 4 cut(s) 202, 349, 532, 568
Tth111I GACNNNGTC 1 cut(s) 434
Van91I CCANNNNNTGG 2 cut(s) 271, 773
VpaK11BI GGWCC 1 cut(s) 362
XapI RAATTY 5 cut(s) 191, 488, 685, 841, 875
XceI RCATGY 1 cut(s) 35
XmnI GAANNNNTTC 2 cut(s) 444, 488
XspI CTAG 2 cut(s) 398, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.