MD14G1016600.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014762
Physical Location & Seq
Forward (+)
1505013 .. 1505927
915 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1016600.v1.1.491

Sequence Viewer

Length: 915 bp
ATGAGAAGCATAGCAACTTGTTACAGCGAGCATGCCATAAAGGTCTCAGATTCATATTGCTCAGGCCCATCAAACCAAGCTTATGTGACCCCAAAATTAACCCCATCAATCCCAAGTGAAGTGACATGTTTATACAGAGCCAAGCTCTCAACCCAGAACCGGATTTTGATCACACTCACCTGGTTCAACAAATTCACATTCCAAGGCCTCACAATCACCATTGGAGACAACAAATCTCAGCCGTCCAAATCGAGTACCAGCACCCACAAGTTGGAAAACCAAAAGGGCACCTTGGCATTCCAATCCTGCAATACCAAGATTGAAGTTTTCTGGGATTTGTCCACTGCCAATTATGAATCCGGACCGGAACCGGCAACCGGGTTTTTCCTCATGGTTCTGGCTGACTTGGAACTCTGCCTCCTCCTCGGCGACAAGGTCGAACAAGCTTTGGACCTGGAGAAGTACAAAACCATCATGTCACAAAAAATATTCACATTGGTTTCTAGGAGTGAGCATTTCTCAGGCAACGCTGTGTATGCAACAAAAGCTCAATTCTGTGACACTGGAGTATCACATGACATTGTGATCAAGTGTGATCAAACCGAAGAACTGGGCTCGAAAACCCCGGTTTTATCTGTCTGCATTGACAAGAAGAAGATTTTTCAGGTGAAGAGGTTGAGGTGGAATTTCAGAGGGAACCAAACAATTTTTTTGGATGGTTTACTGGTGGATTTTATGTGGGATATTCATGACTGGTTGTTCAATCCAAAATCTGGAATTGCTGTGTTTATGTTCAGGACAAGGAGTGGATTGGATTCCAGGCTCTGGTTGGAAGACAAGAATTTGGAAAAGAAAGGGCAAGAAAATAGTGATGAATTTTCTTTGTTGATCTGTGCATGTAAGAGCTCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

305

Amino Acids

34.35

Weight (kDa)

7.5

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 38 - 301 2.1e-80 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014762)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17950
malus_domestica MD12G1018600.v1.1 MD14G1016600.v1.1
prunus_persica Prupe.7G116700_v2.0.a1
pyrus_communis pycom12g01540 pycom14g01420
rosa_chinensis RchiOBHm_Chr3g0472211
rosa_laevigata RLG00000024097
rosa_multiflora Rmu_sc0002532.1_g000035
rosa_roxburghii Rroxscaffold_6G00409150
rosa_rugosa Rorug03G0122800
rosa_samantha Rh3AG175200 Rh3BG200200 Rh3CG191700 Rh3CG199000 Rh3DG174200
rosa_wichuraiana Rw3G016610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 287
AccB7I CCANNNNNTGG 3 cut(s) 271, 773, 825
AccIII TCCGGA 1 cut(s) 359
AcsI RAATTY 4 cut(s) 191, 685, 841, 875
AfaI GTAC 2 cut(s) 256, 464
AfiI CCNNNNNNNGG 5 cut(s) 159, 271, 377, 773, 825
AflIII ACRYGT 1 cut(s) 125
AgsI TTSAA 3 cut(s) 187, 323, 763
AjnI CCWGG 3 cut(s) 179, 453, 818
AloI GAACNNNNNNTCC 2 cut(s) 402, 434
AluBI AGCT 5 cut(s) 80, 145, 446, 548, 906
AluI AGCT 5 cut(s) 80, 145, 446, 548, 906
Alw21I GWGCWC 1 cut(s) 908
Alw26I GTCTC 2 cut(s) 49, 219
AlwNI CAGNNNCTG 1 cut(s) 825
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 2 cut(s) 64, 205
ApoI RAATTY 4 cut(s) 191, 685, 841, 875
AspS9I GGNCC 3 cut(s) 65, 362, 451
AsuC2I CCSGG 2 cut(s) 379, 626
AsuHPI GGTGA 3 cut(s) 169, 208, 679
AvaII GGWCC 2 cut(s) 362, 451
BaeGI GKGCMC 1 cut(s) 290
BaeI ACNNNNGTAYC 2 cut(s) 552, 585
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 2 cut(s) 617, 908
BbsI GAAGAC 1 cut(s) 840
Bbv12I GWGCWC 1 cut(s) 908
BccI CCATC 4 cut(s) 76, 112, 479, 710
BceAI ACGGC 1 cut(s) 226
BciT130I CCWGG 3 cut(s) 181, 455, 820
BclI TGATCA 3 cut(s) 168, 585, 595
BcnI CCSGG 2 cut(s) 379, 626
BcoDI GTCTC 2 cut(s) 49, 219
BfaI CTAG 1 cut(s) 504
Bme1390I CCNGG 5 cut(s) 181, 379, 455, 626, 820
Bme18I GGWCC 2 cut(s) 362, 451
BmgT120I GGNCC 3 cut(s) 65, 362, 451
BmiI GGNNCC 3 cut(s) 289, 369, 698
BmrFI CCNGG 5 cut(s) 181, 379, 455, 626, 820
BmrI ACTGGG 1 cut(s) 620
BmuI ACTGGG 1 cut(s) 620
BpiI GAAGAC 1 cut(s) 840
BplI GAGNNNNNCTC 4 cut(s) 129, 161, 503, 535
BpmI CTGGAG 2 cut(s) 476, 585
Bpu10I CCTNAGC 1 cut(s) 61
BpuMI CCSGG 2 cut(s) 379, 626
BsaBI GATNNNNATC 1 cut(s) 167
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 4 cut(s) 202, 291, 424, 624
BsaWI WCCGGW 3 cut(s) 159, 359, 364
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 5 cut(s) 159, 271, 377, 773, 825
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 4 cut(s) 568, 615, 729, 758
Bse8I GATNNNNATC 1 cut(s) 167
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 3 cut(s) 181, 455, 820
BseDI CCNNGG 4 cut(s) 202, 291, 424, 624
BseGI GGATG 1 cut(s) 721
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 5 cut(s) 159, 271, 377, 773, 825
BseMII CTCAG 4 cut(s) 60, 75, 251, 534
BseNI ACTGG 4 cut(s) 568, 615, 729, 758
BseRI GAGGAG 2 cut(s) 410, 413
BseSI GKGCMC 1 cut(s) 290
BshFI GGCC 2 cut(s) 66, 207
BshNI GGYRCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 908
BsiSI CCGG 6 cut(s) 160, 360, 365, 371, 378, 626
BslI CCNNNNNNNGG 5 cut(s) 159, 271, 377, 773, 825
BsmAI GTCTC 2 cut(s) 49, 219
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 2 cut(s) 66, 207
Bso31I GGTCTC 1 cut(s) 49
Bsp1286I GDGCHC 3 cut(s) 290, 617, 908
Bsp13I TCCGGA 1 cut(s) 359
Bsp143I GATC 4 cut(s) 168, 585, 595, 888
BspANI GGCC 2 cut(s) 66, 207
BspCNI CTCAG 4 cut(s) 59, 74, 250, 533
BspEI TCCGGA 1 cut(s) 359
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 3 cut(s) 289, 369, 698
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 49
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 4 cut(s) 568, 615, 729, 758
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 4 cut(s) 202, 291, 424, 624
BssMI GATC 4 cut(s) 168, 585, 595, 888
BssT1I CCWWGG 2 cut(s) 202, 291
Bst2UI CCWGG 3 cut(s) 181, 455, 820
Bst6I CTCTTC 1 cut(s) 665
BstC8I GCNNGC 2 cut(s) 29, 33
BstDEI CTNAG 4 cut(s) 46, 61, 237, 520
BstF5I GGATG 1 cut(s) 721
BstKTI GATC 4 cut(s) 171, 588, 598, 891
BstMAI GTCTC 2 cut(s) 49, 219
BstMBI GATC 4 cut(s) 168, 585, 595, 888
BstMWI GCNNNNNNNGC 2 cut(s) 536, 545
BstNI CCWGG 3 cut(s) 181, 455, 820
BstNSI RCATGY 3 cut(s) 35, 129, 900
BstSCI CCNGG 5 cut(s) 179, 377, 453, 624, 818
BstSLI GKGCMC 1 cut(s) 290
BstV2I GAAGAC 1 cut(s) 840
BsuRI GGCC 2 cut(s) 66, 207
BtsCI GGATG 1 cut(s) 721
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 342, 561
Cac8I GCNNGC 2 cut(s) 29, 33
CaiI CAGNNNCTG 1 cut(s) 825
CciI TCATGA 1 cut(s) 748
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 3 cut(s) 65, 362, 451
CpoI CGGWCCG 1 cut(s) 362
CsiI ACCWGGT 1 cut(s) 179
Csp6I GTAC 2 cut(s) 255, 463
CspI CGGWCCG 1 cut(s) 362
CviAII CATG 7 cut(s) 32, 126, 391, 475, 575, 749, 897
CviQI GTAC 2 cut(s) 255, 463
DdeI CTNAG 4 cut(s) 46, 61, 237, 520
DpnI GATC 4 cut(s) 170, 587, 597, 890
DpnII GATC 4 cut(s) 168, 585, 595, 888
Eam1104I CTCTTC 1 cut(s) 665
EarI CTCTTC 1 cut(s) 665
Ecl136II GAGCTC 1 cut(s) 906
Eco130I CCWWGG 2 cut(s) 202, 291
Eco147I AGGCCT 1 cut(s) 207
Eco24I GRGCYC 2 cut(s) 617, 908
Eco31I GGTCTC 1 cut(s) 49
Eco47I GGWCC 2 cut(s) 362, 451
Eco53kI GAGCTC 1 cut(s) 906
EcoICRI GAGCTC 1 cut(s) 906
EcoRII CCWGG 3 cut(s) 179, 453, 818
EcoT14I CCWWGG 2 cut(s) 202, 291
EcoT38I GRGCYC 2 cut(s) 617, 908
ErhI CCWWGG 2 cut(s) 202, 291
FaeI CATG 7 cut(s) 35, 129, 394, 478, 578, 752, 900
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FatI CATG 7 cut(s) 31, 125, 390, 474, 574, 748, 896
FbaI TGATCA 3 cut(s) 168, 585, 595
FokI GGATG 1 cut(s) 728
FriOI GRGCYC 2 cut(s) 617, 908
FspBI CTAG 1 cut(s) 504
GsuI CTGGAG 2 cut(s) 476, 585
HaeIII GGCC 2 cut(s) 66, 207
HapII CCGG 6 cut(s) 160, 360, 365, 371, 378, 626
Hin1II CATG 7 cut(s) 35, 129, 394, 478, 578, 752, 900
HindIII AAGCTT 2 cut(s) 78, 444
HinfI GANTC 3 cut(s) 50, 356, 815
HpaII CCGG 6 cut(s) 160, 360, 365, 371, 378, 626
HphI GGTGA 3 cut(s) 169, 208, 679
Hpy166II GTNNAC 2 cut(s) 342, 722
Hpy188I TCNGA 3 cut(s) 49, 692, 910
Hpy188III TCNNGA 4 cut(s) 360, 749, 774, 796
Hpy8I GTNNAC 2 cut(s) 342, 722
HpyCH4V TGCA 4 cut(s) 309, 539, 642, 896
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 545
HpyF3I CTNAG 4 cut(s) 46, 61, 237, 520
Hsp92II CATG 7 cut(s) 35, 129, 394, 478, 578, 752, 900
Kpn2I TCCGGA 1 cut(s) 359
Ksp22I TGATCA 3 cut(s) 168, 585, 595
Kzo9I GATC 4 cut(s) 168, 585, 595, 888
MabI ACCWGGT 1 cut(s) 179
MaeI CTAG 1 cut(s) 504
MaeIII GTNAC 5 cut(s) 20, 85, 121, 477, 557
MalI GATC 4 cut(s) 170, 587, 597, 890
MboI GATC 4 cut(s) 168, 585, 595, 888
MboII GAAGA 5 cut(s) 617, 664, 667, 682, 845
MhlI GDGCHC 3 cut(s) 290, 617, 908
MluCI AATT 9 cut(s) 95, 191, 349, 551, 685, 705, 777, 841, 875
MmeI TCCRAC 2 cut(s) 252, 810
MnlI CCTC 8 cut(s) 218, 398, 428, 431, 434, 666, 672, 686
MroI TCCGGA 1 cut(s) 359
MseI TTAA 1 cut(s) 98
MspI CCGG 6 cut(s) 160, 360, 365, 371, 378, 626
MspR9I CCNGG 5 cut(s) 181, 379, 455, 626, 820
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 3 cut(s) 181, 455, 820
MwoI GCNNNNNNNGC 2 cut(s) 536, 545
NciI CCSGG 2 cut(s) 379, 626
NdeII GATC 4 cut(s) 168, 585, 595, 888
NlaIII CATG 7 cut(s) 35, 129, 394, 478, 578, 752, 900
NlaIV GGNNCC 3 cut(s) 289, 369, 698
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 4 cut(s) 85, 121, 477, 557
NspI RCATGY 3 cut(s) 35, 129, 900
PaeI GCATGC 1 cut(s) 35
PagI TCATGA 1 cut(s) 748
PceI AGGCCT 1 cut(s) 207
PciI ACATGT 1 cut(s) 125
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 3 cut(s) 50, 356, 815
PflFI GACNNNGTC 1 cut(s) 434
PflMI CCANNNNNTGG 3 cut(s) 271, 773, 825
PscI ACATGT 1 cut(s) 125
Psp124BI GAGCTC 1 cut(s) 908
Psp6I CCWGG 3 cut(s) 179, 453, 818
PspGI CCWGG 3 cut(s) 179, 453, 818
PspN4I GGNNCC 3 cut(s) 289, 369, 698
PspPI GGNCC 3 cut(s) 65, 362, 451
PstNI CAGNNNCTG 1 cut(s) 825
PsyI GACNNNGTC 1 cut(s) 434
RsaI GTAC 2 cut(s) 256, 464
RsaNI GTAC 2 cut(s) 255, 463
Rsr2I CGGWCCG 1 cut(s) 362
RsrII CGGWCCG 1 cut(s) 362
SacI GAGCTC 1 cut(s) 908
SaqAI TTAA 1 cut(s) 98
Sau3AI GATC 4 cut(s) 168, 585, 595, 888
Sau96I GGNCC 3 cut(s) 65, 362, 451
ScrFI CCNGG 5 cut(s) 181, 379, 455, 626, 820
SduI GDGCHC 3 cut(s) 290, 617, 908
SexAI ACCWGGT 1 cut(s) 179
SinI GGWCC 2 cut(s) 362, 451
SphI GCATGC 1 cut(s) 35
Sse9I AATT 9 cut(s) 95, 191, 349, 551, 685, 705, 777, 841, 875
SseBI AGGCCT 1 cut(s) 207
SspI AATATT 1 cut(s) 489
SspMI CTAG 1 cut(s) 504
SstI GAGCTC 1 cut(s) 908
StuI AGGCCT 1 cut(s) 207
StyD4I CCNGG 5 cut(s) 179, 377, 453, 624, 818
StyI CCWWGG 2 cut(s) 202, 291
TaqI TCGA 3 cut(s) 251, 438, 617
TasI AATT 9 cut(s) 95, 191, 349, 551, 685, 705, 777, 841, 875
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 3 cut(s) 50, 356, 815
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 2 cut(s) 349, 568
TseFI GTSAC 4 cut(s) 85, 121, 477, 557
Tsp45I GTSAC 4 cut(s) 85, 121, 477, 557
TspDTI ATGAA 4 cut(s) 42, 369, 737, 888
TspRI CASTG 2 cut(s) 349, 568
Tth111I GACNNNGTC 1 cut(s) 434
Van91I CCANNNNNTGG 3 cut(s) 271, 773, 825
VpaK11BI GGWCC 2 cut(s) 362, 451
XapI RAATTY 4 cut(s) 191, 685, 841, 875
XceI RCATGY 3 cut(s) 35, 129, 900
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XspI CTAG 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.