Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006005

Physical Location & Seq

Genomic Coordinates Reverse (-)

3994051 .. 3999058

Gene Structure 5008 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1037100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1410 bp

ATGTCCGGCTGCGCTCAAGCTCCCAACCGCCAACCAGCGCCGGCGACGGCACCCACAGGCGGCGGCGCCCAGATCCTGCCGCCGTTGAGGCGTCACCTCGCGTTCGAGTCCACAAAGCCGCAGCCGTTCCCTCCCGACAATTACCACCGATTCGCGGGCGGTGCTCGCAGAGCTGGAGACCAAGAACCTGAAGCTATTTATGTTCGATCTCCTCAACTTACTCGGAAGGACACCAATGAAGTTGAGTACAGTGATTGGACCTGCAGTCCTGGAAATACTAATATTATTCAGACTCCCCTTCAGACTCCTCCAATGTCTACAAAAGGGGGAAGGGTAAACAATAGGTCAAAGGCTTCCAAGGGAGGTAGATCTGGACCTCAGACTCCTGTGTCAAATGTTGGTTCCCCTTCTACTCCAGCCGGAAGCTGTCGTTATGACAGTTCCTTAGGTCTGTTGACAAAAAAGTTCATCAATTTGATTAAGCGTGCAGAGGATGGAATTCTTGATCTAAACAAAGCAGCGGAAACTTTGGAGGTTCAGAAGAGGCGGATATATGACATTACAAATGTTTTGGAAGGAATAGGTCTCATTGAAAAGAAGCTGAAGAACAGGATACGTTGGAAGGGATTTGATGCTTCAATGCCAGGAGAGTTGGATGGTGATCTCTCTATATTACAGGCAGAAGTTGAAAATCTATCCTTACAAGAGCGCAGATTAGATGACCGAATAAGAGAGATGCAAGAAAAGTTGAGGGATTTGAGCGAAGATGAAAACAACCGAAAGTGGCTTTTTGTGACTGAAGATGACATTAAGGGCCTACCTTGCTTCCACAATGAAACCCTGATAGCAATCAAAGCTCCACATGGAACCACCTTGGAAGTCCCAGATCCTGATGAAGCTGTTGACTATCTACAGAGGAGATACAGGATAATACTTAGAAGCACAATGGGACCCATTGATGTCTACCTTGTCAGTCAATTTGAGGAAAAGTTTGAGGATGTGCATGGAGCTGAGCCACCTGTGAGCTGCCCAATGGCTTCTGGTTCTGGGTCCAGTGAACACCCAATAACAGAAGTGGTAACTGTTGAAAGCAGTGGGAAGGAAATTGAGCCTTGGCTGCAACAGGCTCATCACACGTGCTCTGATTTAAACGCTTCTCAGGAGTTTGCTGGGGGAATGATGAAGATCGTTCCTTCTGATGTTGATAATGATGCAGACTACTGGCTTTTGTCTGATGCCGAAGTTAGCATTACAGATATGTGGAGGACGGATTCTGGTGTTGGATGGAATGGGACAGACATTCTTCATGCTGACTTTGGAGTGACTGATGTTAGTACACCAAGGCCACAAACTCCGCCATCTAGGGTTGTTGAAGTCCCATCTCATGCAGTTAACTTCGCTCAAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0045787 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051445 GO:0051446 GO:0051726 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko01522 ko03018 ko04110 ko04218 ko04934 ko05161 ko05166 ko05167 ko05200 ko05206 ko05212 ko05214 ko05215 ko05218 ko05219 ko05220 ko05222 ko05223 ko05224 ko05225 ko05226 map01522 map03018 map04110 map04218 map04934 map05161 map05166 map05167 map05200 map05206 map05212 map05214 map05215 map05218 map05219 map05220 map05222 map05223 map05224 map05225 map05226

Pfam Domains

Protein Families

E2F_CC-MB E2F_TDP

Protein Analysis

470

Amino Acids

51.81

Weight (kDa)

5.25

Isoelectric Point (pI)

58.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_E2F_TDP	PF02319	146 - 209	2.7e-27	E2F/DP family winged-helix DNA-binding domain
E2F_CC-MB	PF16421	225 - 325	1.1e-34	E2F transcription factor CC-MB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36010 AT2G36010 AT2G36010
fragaria_vesca	FvH4_6g19970 FvH4_6g19970 FvH4_6g19970 FvH4_6g19970 FvH4_6g19970 FvH4_6g19970 FvH4_6g19970
malus_domestica	MD12G1037100.v1.1 MD14G1036100.v1.1
prunus_persica	Prupe.7G098100_v2.0.a1
pyrus_communis	pycom12g03210 pycom14g03180
rosa_chinensis	RchiOBHm_Chr3g0475421
rosa_laevigata	RLG00000023845
rosa_multiflora	Rmu_sc0016637.1_g000006 Rmu_sc0018174.1_g000003
rosa_roxburghii	Rroxscaffold_6G00406320
rosa_rugosa	Rorug03G0148700 Rorug03G0148700
rosa_samantha	Rh3AG198900 Rh3BG228700 Rh3CG223100 Rh3DG224100
rosa_wichuraiana	Rw3G018050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	388
Acc36I	ACCTGC	1 cut(s)	269
AccB1I	GGYRCC	2 cut(s)	49, 65
AccI	GTMKAC	2 cut(s)	317, 963
AccII	CGCG	2 cut(s)	101, 155
AclWI	GGATC	2 cut(s)	67, 881
AcsI	RAATTY	1 cut(s)	498
AcuI	CTGAAG	4 cut(s)	210, 284, 623, 819
AcvI	CACGTG	1 cut(s)	1137
AcyI	GRCGYC	2 cut(s)	66, 91
AfaI	GTAC	2 cut(s)	248, 1336
AfiI	CCNNNNNNNGG	2 cut(s)	59, 154
AflIII	ACRYGT	1 cut(s)	1134
AgsI	TTSAA	5 cut(s)	593, 639, 689, 1088, 1373
AhdI	GACNNNNNGTC	1 cut(s)	264
AjnI	CCWGG	2 cut(s)	268, 643
AluBI	AGCT	9 cut(s)	20, 173, 194, 426, 601, 857, 899, 1010, 1026
AluI	AGCT	9 cut(s)	20, 173, 194, 426, 601, 857, 899, 1010, 1026
Alw21I	GWGCWC	2 cut(s)	166, 1142
Alw26I	GTCTC	2 cut(s)	171, 590
AlwI	GGATC	2 cut(s)	67, 881
AlwNI	CAGNNNCTG	2 cut(s)	76, 890
AoxI	GGCC	2 cut(s)	814, 1343
ApeKI	GCWGC	5 cut(s)	9, 121, 518, 1026, 1117
ApoI	RAATTY	1 cut(s)	498
AspLEI	GCGC	4 cut(s)	14, 40, 68, 711
AspS9I	GGNCC	5 cut(s)	258, 374, 814, 950, 1050
AsuHPI	GGTGA	2 cut(s)	86, 671
AvaII	GGWCC	4 cut(s)	258, 374, 950, 1050
AxyI	CCTNAGG	1 cut(s)	445
BanI	GGYRCC	2 cut(s)	49, 65
BbrPI	CACGTG	1 cut(s)	1137
Bbv12I	GWGCWC	2 cut(s)	166, 1142
BbvI	GCAGC	4 cut(s)	133, 530, 1013, 1104
BccI	CCATC	5 cut(s)	488, 650, 1278, 1366, 1387
BceAI	ACGGC	3 cut(s)	63, 67, 109
BciT130I	CCWGG	2 cut(s)	270, 645
BciVI	GTATCC	1 cut(s)	606
BcoDI	GTCTC	2 cut(s)	171, 590
BfaI	CTAG	1 cut(s)	1362
BfmI	CTRYAG	2 cut(s)	262, 911
BfoI	RGCGCY	2 cut(s)	41, 69
BfuAI	ACCTGC	1 cut(s)	269
BfuI	GTATCC	1 cut(s)	606
BglI	GCCNNNNNGGC	1 cut(s)	88
BglII	AGATCT	1 cut(s)	368
BisI	GCNGC	9 cut(s)	10, 61, 64, 80, 119, 122, 519, 1027, 1118
BlpI	GCTNAGC	1 cut(s)	1011
BlsI	GCNGC	9 cut(s)	11, 62, 65, 81, 120, 123, 520, 1028, 1119
Bme1390I	CCNGG	2 cut(s)	270, 645
Bme18I	GGWCC	4 cut(s)	258, 374, 950, 1050
BmeRI	GACNNNNNGTC	1 cut(s)	264
BmgT120I	GGNCC	5 cut(s)	258, 374, 814, 950, 1050
BmiI	GGNNCC	7 cut(s)	51, 67, 403, 868, 951, 952, 1051
BmrFI	CCNGG	2 cut(s)	270, 645
BmsI	GCATC	4 cut(s)	622, 726, 1201, 1225
BpmI	CTGGAG	2 cut(s)	195, 399
Bpu1102I	GCTNAGC	1 cut(s)	1011
BsaAI	YACGTR	1 cut(s)	1137
BsaBI	GATNNNNATC	3 cut(s)	660, 848, 1184
BsaHI	GRCGYC	2 cut(s)	66, 91
BsaI	GGTCTC	2 cut(s)	171, 590
BsaJI	CCNNGG	4 cut(s)	357, 873, 1112, 1340
Bsc4I	CCNNNNNNNGG	2 cut(s)	59, 154
Bse118I	RCCGGY	1 cut(s)	40
Bse1I	ACTGG	2 cut(s)	1053, 1226
Bse21I	CCTNAGG	1 cut(s)	445
Bse8I	GATNNNNATC	3 cut(s)	660, 848, 1184
BseBI	CCWGG	2 cut(s)	270, 645
BseDI	CCNNGG	4 cut(s)	357, 873, 1112, 1340
BseGI	GGATG	4 cut(s)	499, 661, 1003, 1289
BseJI	GATNNNNATC	3 cut(s)	660, 848, 1184
BseLI	CCNNNNNNNGG	2 cut(s)	59, 154
BseMII	CTCAG	3 cut(s)	392, 1002, 1172
BseNI	ACTGG	2 cut(s)	1053, 1226
BseRI	GAGGAG	3 cut(s)	201, 297, 931
BseXI	GCAGC	4 cut(s)	133, 530, 1013, 1104
BseYI	CCCAGC	1 cut(s)	1169
BsgI	GTGCAG	1 cut(s)	507
Bsh1236I	CGCG	2 cut(s)	101, 155
BshFI	GGCC	2 cut(s)	816, 1345
BshNI	GGYRCC	2 cut(s)	49, 65
BsiHKAI	GWGCWC	2 cut(s)	166, 1142
BsiSI	CCGG	3 cut(s)	6, 41, 420
BslFI	GGGAC	4 cut(s)	866, 963, 1306, 1361
BslI	CCNNNNNNNGG	2 cut(s)	59, 154
BsmAI	GTCTC	2 cut(s)	171, 590
BsmFI	GGGAC	4 cut(s)	866, 963, 1306, 1361
BsnI	GGCC	2 cut(s)	816, 1345
Bso31I	GGTCTC	2 cut(s)	171, 590
Bsp1286I	GDGCHC	2 cut(s)	166, 1142
Bsp143I	GATC	7 cut(s)	72, 206, 368, 505, 661, 886, 1185
Bsp1720I	GCTNAGC	1 cut(s)	1011
BspANI	GGCC	2 cut(s)	816, 1345
BspCNI	CTCAG	3 cut(s)	391, 1003, 1171
BspFNI	CGCG	2 cut(s)	101, 155
BspLI	GGNNCC	7 cut(s)	51, 67, 403, 868, 951, 952, 1051
BspMAI	CTGCAG	1 cut(s)	266
BspMI	ACCTGC	1 cut(s)	269
BspPI	GGATC	2 cut(s)	67, 881
BspT107I	GGYRCC	2 cut(s)	49, 65
BspTNI	GGTCTC	2 cut(s)	171, 590
BsrFI	RCCGGY	1 cut(s)	40
BsrI	ACTGG	2 cut(s)	1053, 1226
BssAI	RCCGGY	1 cut(s)	40
BssECI	CCNNGG	4 cut(s)	357, 873, 1112, 1340
BssMI	GATC	7 cut(s)	72, 206, 368, 505, 661, 886, 1185
BssNI	GRCGYC	2 cut(s)	66, 91
BssT1I	CCWWGG	4 cut(s)	357, 873, 1112, 1340
Bst2UI	CCWGG	2 cut(s)	270, 645
Bst4CI	ACNGT	3 cut(s)	251, 440, 1084
Bst6I	CTCTTC	1 cut(s)	536
BstACI	GRCGYC	2 cut(s)	66, 91
BstBAI	YACGTR	1 cut(s)	1137
BstC8I	GCNNGC	4 cut(s)	42, 157, 166, 486
BstDEI	CTNAG	5 cut(s)	378, 445, 935, 1011, 1158
BstF5I	GGATG	4 cut(s)	499, 661, 1003, 1289
BstFNI	CGCG	2 cut(s)	101, 155
BstH2I	RGCGCY	2 cut(s)	41, 69
BstHHI	GCGC	4 cut(s)	14, 40, 68, 711
BstKTI	GATC	7 cut(s)	75, 209, 371, 508, 664, 889, 1188
BstMAI	GTCTC	2 cut(s)	171, 590
BstMBI	GATC	7 cut(s)	72, 206, 368, 505, 661, 886, 1185
BstMWI	GCNNNNNNNGC	7 cut(s)	88, 161, 165, 170, 822, 854, 1117
BstNI	CCWGG	2 cut(s)	270, 645
BstSCI	CCNGG	2 cut(s)	268, 643
BstSFI	CTRYAG	2 cut(s)	262, 911
BstUI	CGCG	2 cut(s)	101, 155
BstV1I	GCAGC	4 cut(s)	133, 530, 1013, 1104
BstX2I	RGATCY	3 cut(s)	72, 368, 886
BstYI	RGATCY	3 cut(s)	72, 368, 886
Bsu36I	CCTNAGG	1 cut(s)	445
BsuI	GTATCC	1 cut(s)	606
BsuRI	GGCC	2 cut(s)	816, 1345
BtsCI	GGATG	4 cut(s)	499, 661, 1003, 1289
BtsI	GCAGTG	1 cut(s)	1099
BtsIMutI	CAGTG	3 cut(s)	256, 1060, 1099
BveI	ACCTGC	1 cut(s)	269
Cac8I	GCNNGC	4 cut(s)	42, 157, 166, 486
CaiI	CAGNNNCTG	2 cut(s)	76, 890
CfoI	GCGC	4 cut(s)	14, 40, 68, 711
Cfr10I	RCCGGY	1 cut(s)	40
Cfr13I	GGNCC	5 cut(s)	258, 374, 814, 950, 1050
CseI	GACGC	1 cut(s)	80
Csp6I	GTAC	2 cut(s)	247, 1335
CviAII	CATG	4 cut(s)	863, 1004, 1307, 1385
CviQI	GTAC	2 cut(s)	247, 1335
DdeI	CTNAG	5 cut(s)	378, 445, 935, 1011, 1158
DinI	GGCGCC	1 cut(s)	67
DpnI	GATC	7 cut(s)	74, 208, 370, 507, 663, 888, 1187
DpnII	GATC	7 cut(s)	72, 206, 368, 505, 661, 886, 1185
DraI	TTTAAA	1 cut(s)	1149
DrdI	GACNNNNNNGTC	1 cut(s)	388
DriI	GACNNNNNGTC	1 cut(s)	264
DseDI	GACNNNNNNGTC	1 cut(s)	388
Eam1104I	CTCTTC	1 cut(s)	536
Eam1105I	GACNNNNNGTC	1 cut(s)	264
EarI	CTCTTC	1 cut(s)	536
EciI	GGCGGA	2 cut(s)	562, 1344
Eco130I	CCWWGG	4 cut(s)	357, 873, 1112, 1340
Eco31I	GGTCTC	2 cut(s)	171, 590
Eco47I	GGWCC	4 cut(s)	258, 374, 950, 1050
Eco57I	CTGAAG	4 cut(s)	210, 284, 623, 819
Eco72I	CACGTG	1 cut(s)	1137
Eco81I	CCTNAGG	1 cut(s)	445
EcoO109I	RGGNCCY	2 cut(s)	814, 950
EcoRI	GAATTC	1 cut(s)	498
EcoRII	CCWGG	2 cut(s)	268, 643
EcoT14I	CCWWGG	4 cut(s)	357, 873, 1112, 1340
EgeI	GGCGCC	1 cut(s)	67
EheI	GGCGCC	1 cut(s)	67
ErhI	CCWWGG	4 cut(s)	357, 873, 1112, 1340
FaeI	CATG	4 cut(s)	866, 1007, 1310, 1388
FaqI	GGGAC	4 cut(s)	866, 963, 1306, 1361
FatI	CATG	4 cut(s)	862, 1003, 1306, 1384
FauI	CCCGC	1 cut(s)	148
FblI	GTMKAC	2 cut(s)	317, 963
Fnu4HI	GCNGC	9 cut(s)	10, 61, 64, 80, 119, 122, 519, 1027, 1118
FokI	GGATG	4 cut(s)	506, 668, 1010, 1296
Fsp4HI	GCNGC	9 cut(s)	10, 61, 64, 80, 119, 122, 519, 1027, 1118
FspBI	CTAG	1 cut(s)	1362
GlaI	GCGC	4 cut(s)	13, 39, 67, 710
GluI	GCNGC	9 cut(s)	10, 61, 64, 80, 119, 122, 519, 1027, 1118
GsaI	CCCAGC	1 cut(s)	1173
GsuI	CTGGAG	2 cut(s)	195, 399
HaeII	RGCGCY	2 cut(s)	41, 69
HaeIII	GGCC	2 cut(s)	816, 1345
HapII	CCGG	3 cut(s)	6, 41, 420
HgaI	GACGC	1 cut(s)	80
HhaI	GCGC	4 cut(s)	14, 40, 68, 711
Hin1I	GRCGYC	2 cut(s)	66, 91
Hin1II	CATG	4 cut(s)	866, 1007, 1310, 1388
Hin6I	GCGC	4 cut(s)	12, 38, 66, 709
HinP1I	GCGC	4 cut(s)	12, 38, 66, 709
HincII	GTYRAC	3 cut(s)	456, 904, 1393
HindII	GTYRAC	3 cut(s)	456, 904, 1393
HinfI	GANTC	6 cut(s)	107, 150, 292, 304, 382, 1271
HpaI	GTTAAC	1 cut(s)	1393
HpaII	CCGG	3 cut(s)	6, 41, 420
HphI	GGTGA	2 cut(s)	86, 671
Hpy166II	GTNNAC	9 cut(s)	111, 318, 337, 456, 904, 964, 1058, 1337, 1393
Hpy188I	TCNGA	8 cut(s)	225, 291, 303, 381, 540, 1144, 1198, 1234
Hpy188III	TCNNGA	5 cut(s)	134, 372, 503, 890, 1160
Hpy8I	GTNNAC	9 cut(s)	111, 318, 337, 456, 904, 964, 1058, 1337, 1393
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	8 cut(s)	220, 308, 324, 417, 569, 616, 1093, 1203
HpyCH4III	ACNGT	3 cut(s)	251, 440, 1084
HpyCH4IV	ACGT	2 cut(s)	616, 1136
HpyCH4V	TGCA	7 cut(s)	264, 488, 739, 1003, 1120, 1214, 1388
HpyF10VI	GCNNNNNNNGC	7 cut(s)	88, 161, 165, 170, 822, 854, 1117
HpyF3I	CTNAG	5 cut(s)	378, 445, 935, 1011, 1158
HpySE526I	ACGT	2 cut(s)	616, 1136
Hsp92I	GRCGYC	2 cut(s)	66, 91
Hsp92II	CATG	4 cut(s)	866, 1007, 1310, 1388
HspAI	GCGC	4 cut(s)	12, 38, 66, 709
KasI	GGCGCC	1 cut(s)	65
KflI	GGGWCCC	1 cut(s)	950
KroI	GCCGGC	1 cut(s)	40
KroNI	GCCGGC	1 cut(s)	42
KspAI	GTTAAC	1 cut(s)	1393
Kzo9I	GATC	7 cut(s)	72, 206, 368, 505, 661, 886, 1185
LmnI	GCTCC	3 cut(s)	25, 862, 1007
Lsp1109I	GCAGC	4 cut(s)	133, 530, 1013, 1104
LweI	GCATC	4 cut(s)	622, 726, 1201, 1225
MaeI	CTAG	1 cut(s)	1362
MaeII	ACGT	2 cut(s)	616, 1136
MaeIII	GTNAC	4 cut(s)	92, 793, 1078, 1321
MalI	GATC	7 cut(s)	74, 208, 370, 507, 663, 888, 1187
MboI	GATC	7 cut(s)	72, 206, 368, 505, 661, 886, 1185
MboII	GAAGA	6 cut(s)	553, 616, 776, 812, 1195, 1295
MflI	RGATCY	3 cut(s)	72, 368, 886
MhlI	GDGCHC	2 cut(s)	166, 1142
MluCI	AATT	5 cut(s)	139, 472, 498, 977, 1104
Mly113I	GGCGCC	1 cut(s)	66
MlyI	GAGTC	4 cut(s)	116, 286, 298, 376
MmeI	TCCRAC	3 cut(s)	599, 633, 1261
MreI	CGCCGGCG	1 cut(s)	40
MroNI	GCCGGC	1 cut(s)	40
MseI	TTAA	4 cut(s)	480, 810, 1148, 1392
MspA1I	CMGCKG	1 cut(s)	521
MspI	CCGG	3 cut(s)	6, 41, 420
MspR9I	CCNGG	2 cut(s)	270, 645
MvaI	CCWGG	2 cut(s)	270, 645
MvnI	CGCG	2 cut(s)	101, 155
MwoI	GCNNNNNNNGC	7 cut(s)	88, 161, 165, 170, 822, 854, 1117
NaeI	GCCGGC	1 cut(s)	42
NarI	GGCGCC	1 cut(s)	66
NdeII	GATC	7 cut(s)	72, 206, 368, 505, 661, 886, 1185
NgoMIV	GCCGGC	1 cut(s)	40
NlaIII	CATG	4 cut(s)	866, 1007, 1310, 1388
NlaIV	GGNNCC	7 cut(s)	51, 67, 403, 868, 951, 952, 1051
NmuCI	GTSAC	3 cut(s)	92, 793, 1321
PdiI	GCCGGC	1 cut(s)	42
PfeI	GAWTC	2 cut(s)	150, 1271
PfoI	TCCNGGA	1 cut(s)	268
PkrI	GCNGC	9 cut(s)	11, 62, 65, 81, 120, 123, 520, 1028, 1119
PleI	GAGTC	4 cut(s)	115, 286, 298, 376
PluTI	GGCGCC	1 cut(s)	69
PmaCI	CACGTG	1 cut(s)	1137
PmlI	CACGTG	1 cut(s)	1137
PpsI	GAGTC	4 cut(s)	115, 286, 298, 376
Ppu21I	YACGTR	1 cut(s)	1137
PpuMI	RGGWCCY	1 cut(s)	950
Psp5II	RGGWCCY	1 cut(s)	950
Psp6I	CCWGG	2 cut(s)	268, 643
PspCI	CACGTG	1 cut(s)	1137
PspFI	CCCAGC	1 cut(s)	1169
PspGI	CCWGG	2 cut(s)	268, 643
PspN4I	GGNNCC	7 cut(s)	51, 67, 403, 868, 951, 952, 1051
PspPI	GGNCC	5 cut(s)	258, 374, 814, 950, 1050
PspPPI	RGGWCCY	1 cut(s)	950
PstI	CTGCAG	1 cut(s)	266
PstNI	CAGNNNCTG	2 cut(s)	76, 890
PsuI	RGATCY	3 cut(s)	72, 368, 886
RsaI	GTAC	2 cut(s)	248, 1336
RsaNI	GTAC	2 cut(s)	247, 1335
SaqAI	TTAA	4 cut(s)	480, 810, 1148, 1392
SatI	GCNGC	9 cut(s)	10, 61, 64, 80, 119, 122, 519, 1027, 1118
Sau3AI	GATC	7 cut(s)	72, 206, 368, 505, 661, 886, 1185
Sau96I	GGNCC	5 cut(s)	258, 374, 814, 950, 1050
SchI	GAGTC	4 cut(s)	116, 286, 298, 376
ScrFI	CCNGG	2 cut(s)	270, 645
SduI	GDGCHC	2 cut(s)	166, 1142
SfaNI	GCATC	4 cut(s)	622, 726, 1201, 1225
SfcI	CTRYAG	2 cut(s)	262, 911
SfoI	GGCGCC	1 cut(s)	67
SgrAI	CRCCGGYG	1 cut(s)	40
SinI	GGWCC	4 cut(s)	258, 374, 950, 1050
SmlI	CTYRAG	1 cut(s)	15
SmoI	CTYRAG	1 cut(s)	15
Sse9I	AATT	5 cut(s)	139, 472, 498, 977, 1104
SspDI	GGCGCC	1 cut(s)	65
SspI	AATATT	1 cut(s)	283
SspMI	CTAG	1 cut(s)	1362
StyD4I	CCNGG	2 cut(s)	268, 643
StyI	CCWWGG	4 cut(s)	357, 873, 1112, 1340
TaaI	ACNGT	3 cut(s)	251, 440, 1084
TaiI	ACGT	2 cut(s)	619, 1139
TaqI	TCGA	2 cut(s)	105, 205
TaqII	GACCGA	1 cut(s)	738
TasI	AATT	5 cut(s)	139, 472, 498, 977, 1104
TatI	WGTACW	2 cut(s)	246, 1334
TauI	GCSGC	4 cut(s)	63, 66, 82, 121
TfiI	GAWTC	2 cut(s)	150, 1271
Tru1I	TTAA	4 cut(s)	480, 810, 1148, 1392
Tru9I	TTAA	4 cut(s)	480, 810, 1148, 1392
TscAI	CASTG	3 cut(s)	256, 1060, 1099
TseFI	GTSAC	3 cut(s)	92, 793, 1321
TseI	GCWGC	5 cut(s)	9, 121, 518, 1026, 1117
Tsp45I	GTSAC	3 cut(s)	92, 793, 1321
TspDTI	ATGAA	7 cut(s)	252, 457, 783, 849, 909, 1196, 1295
TspGWI	ACGGA	1 cut(s)	1283
TspRI	CASTG	3 cut(s)	256, 1060, 1099
VpaK11BI	GGWCC	4 cut(s)	258, 374, 950, 1050
XapI	RAATTY	1 cut(s)	498
XmiI	GTMKAC	2 cut(s)	317, 963
XspI	CTAG	1 cut(s)	1362

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.