Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006005

Physical Location & Seq

Genomic Coordinates Reverse (-)

3249419 .. 3253868

Gene Structure 4450 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1036100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGTCCGGCTTCGCTCGAGCACCCAACCGCCAAGCACCGCCGGCGACGGCAGCTAACGGCGGCGGCGGCGGCTCCCAGATTCTGCCGCCGTTGAGGCGTCACCTCGCGTTCGAGTCCACGAAGCCGCAGCCGTTCCCTCCCGACAATTATCATCGATTCTCCGGCGGTCCTCGCAGAGCTGGGGACCAAGAACCTGAAGCTATTCTTGTTCGATCTCCTCAACTTACTCAGAAGGACAACAATGAAGTAGGGTACAGTGATTGGACCAGCAGTCCTGGAAATACTAATGTTATACAGAGTCCCCTTCAGACTCCTGTGTCTGCAAAAGGGGGAAGGGTAAACAACAGGTCAAAGGCTTCCAAGGGTGGTAGATCCGGACCTCAGACGCCTGTGTCAATTGTTGGTTCCCCTTCTACTCCAGCCGGAAGCTGTCGTTATGACAGTTCTCTAGGTCTGTTGACAAAAAAGTTCATCAATTTGATTAAGCGTGCAGAGGATGGAATTCTTGATCTAAACAAAGCAGCAGAAACTTTGGAGGTTCAGAAGAGGCGGATATATGACATTACAAATGTTTTGGAAGGAATAGGTCTCATTGAAAAGAAGCTGAAGAACAGGATACGTTGGAAGGGATTTGATGCTTCAATGCCAGGGGAGTTGGATGGTGATCTCTCTATATTACAGGCAGAAGTTGAAAACCTATCCTTACAAGAGCGCAGATTAGATGATCGAATAAGAGAGATGCAAGAAAAGTTGAGGGATTTGAGCGAAAATGAAAACAACCGAAAGTGGCTTTTTGTGACTGAAGATGACATTAAGGGCCTACCTTGCTTCCACAATGAAACCCTGATAGCAATTAAAGCTCCACATGGAACCACCTTGGAAGTCCCAGATCCTGATGAAGCTGTTGACTATCCACAGAGGAGATACAGGATAATACTTAGAAGCACAATGGGGCCCATTGATGTCTACCTTGTCAGTCAATTTGAGGAAAAGTTCGAGGATGTGCATGGTGCTGAACCACCTGTGAACTTCCCAATTGCTTCTGGTTCTGGGTCCAACGAACATTCAACAACAGAAGTGGTAACTGTGGAAAGCAGCGGGAAGGAAATTGAGCCTTGGCTGCAACAGGTTCATCACACGTGCTCTGACATGAACGCTTCTCAGGAGTTTGCTGGGGGAATGATGAAGATTGTTCCTTCTGATGTTGATAATGATGCAGACTACTGGCTTTTGTCTGATGCCGAAGTTAGCATTACAGATATGTGGAGGACGGATTCTGGTGTTGGATGGAATGGGACGGACATTCTTCATGCTGACTTTGGAGTGACTGATGTTAGTACTTCAAGGCCGCAAACTCCTCCATCTAGGGTTGTTGAAGTCCCATCTCATGCTGTTAACTTCACTCAAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0045787 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051445 GO:0051446 GO:0051726 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko01522 ko03018 ko04110 ko04218 ko04934 ko05161 ko05166 ko05167 ko05200 ko05206 ko05212 ko05214 ko05215 ko05218 ko05219 ko05220 ko05222 ko05223 ko05224 ko05225 ko05226 map01522 map03018 map04110 map04218 map04934 map05161 map05166 map05167 map05200 map05206 map05212 map05214 map05215 map05218 map05219 map05220 map05222 map05223 map05224 map05225 map05226

Pfam Domains

Protein Families

E2F_CC-MB E2F_TDP

Protein Analysis

471

Amino Acids

51.8

Weight (kDa)

5.37

Isoelectric Point (pI)

57.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_E2F_TDP	PF02319	147 - 210	2.7e-27	E2F/DP family winged-helix DNA-binding domain
E2F_CC-MB	PF16421	226 - 326	9.5e-35	E2F transcription factor CC-MB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36010 AT2G36010 AT2G36010
fragaria_vesca	FvH4_6g19970 FvH4_6g19970 FvH4_6g19970 FvH4_6g19970 FvH4_6g19970 FvH4_6g19970 FvH4_6g19970
malus_domestica	MD12G1037100.v1.1 MD14G1036100.v1.1
prunus_persica	Prupe.7G098100_v2.0.a1
pyrus_communis	pycom12g03210 pycom14g03180
rosa_chinensis	RchiOBHm_Chr3g0475421
rosa_laevigata	RLG00000023845
rosa_multiflora	Rmu_sc0016637.1_g000006 Rmu_sc0018174.1_g000003
rosa_roxburghii	Rroxscaffold_6G00406320
rosa_rugosa	Rorug03G0148700 Rorug03G0148700
rosa_samantha	Rh3AG198900 Rh3BG228700 Rh3CG223100 Rh3DG224100
rosa_wichuraiana	Rw3G018050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	316, 391
AccI	GTMKAC	1 cut(s)	966
AccII	CGCG	1 cut(s)	107
AccIII	TCCGGA	1 cut(s)	374
AclWI	GGATC	2 cut(s)	366, 884
AcsI	RAATTY	1 cut(s)	501
AcuI	CTGAAG	4 cut(s)	216, 290, 626, 822
AcvI	CACGTG	1 cut(s)	1140
AcyI	GRCGYC	2 cut(s)	97, 386
AfaI	GTAC	2 cut(s)	254, 1339
AflIII	ACRYGT	1 cut(s)	1137
AgsI	TTSAA	6 cut(s)	596, 642, 692, 1068, 1344, 1376
AhdI	GACNNNNNGTC	1 cut(s)	270
AjnI	CCWGG	2 cut(s)	274, 646
AluBI	AGCT	7 cut(s)	53, 179, 200, 429, 604, 860, 902
AluI	AGCT	7 cut(s)	53, 179, 200, 429, 604, 860, 902
Alw21I	GWGCWC	2 cut(s)	22, 1145
Alw26I	GTCTC	1 cut(s)	593
AlwI	GGATC	2 cut(s)	366, 884
AlwNI	CAGNNNCTG	2 cut(s)	82, 893
Ama87I	CYCGRG	1 cut(s)	15
Aor13HI	TCCGGA	1 cut(s)	374
AoxI	GGCC	3 cut(s)	817, 953, 1346
ApaI	GGGCCC	1 cut(s)	957
ApeKI	GCWGC	5 cut(s)	50, 127, 521, 1095, 1120
ApoI	RAATTY	1 cut(s)	501
Asp700I	GAANNNNTTC	1 cut(s)	201
AspLEI	GCGC	1 cut(s)	714
AspS9I	GGNCC	8 cut(s)	167, 184, 264, 377, 817, 953, 954, 1053
AsuHPI	GGTGA	2 cut(s)	92, 674
AvaI	CYCGRG	1 cut(s)	15
AvaII	GGWCC	5 cut(s)	167, 184, 264, 377, 1053
BaeGI	GKGCMC	1 cut(s)	957
BanII	GRGCYC	1 cut(s)	957
BbrPI	CACGTG	1 cut(s)	1140
Bbv12I	GWGCWC	2 cut(s)	22, 1145
BbvI	GCAGC	5 cut(s)	62, 139, 533, 1107, 1107
BccI	CCATC	5 cut(s)	491, 653, 1281, 1369, 1390
BceAI	ACGGC	4 cut(s)	63, 73, 73, 115
BciT130I	CCWGG	2 cut(s)	276, 648
BciVI	GTATCC	1 cut(s)	609
BcoDI	GTCTC	1 cut(s)	593
BfaI	CTAG	2 cut(s)	449, 1365
BfuI	GTATCC	1 cut(s)	609
BglI	GCCNNNNNGGC	1 cut(s)	94
BmcAI	AGTACT	1 cut(s)	1339
Bme1390I	CCNGG	2 cut(s)	276, 648
Bme18I	GGWCC	5 cut(s)	167, 184, 264, 377, 1053
BmeRI	GACNNNNNGTC	1 cut(s)	270
BmeT110I	CYCGRG	1 cut(s)	15
BmgT120I	GGNCC	8 cut(s)	167, 184, 264, 377, 817, 953, 954, 1053
BmiI	GGNNCC	7 cut(s)	73, 185, 406, 871, 954, 955, 1054
BmrFI	CCNGG	2 cut(s)	276, 648
BmsI	GCATC	4 cut(s)	625, 729, 1204, 1228
BpmI	CTGGAG	1 cut(s)	402
Bsa29I	ATCGAT	1 cut(s)	154
BsaAI	YACGTR	1 cut(s)	1140
BsaBI	GATNNNNATC	1 cut(s)	663
BsaHI	GRCGYC	2 cut(s)	97, 386
BsaI	GGTCTC	1 cut(s)	593
BsaJI	CCNNGG	4 cut(s)	360, 647, 876, 1115
BsaWI	WCCGGW	1 cut(s)	374
Bse118I	RCCGGY	1 cut(s)	40
Bse1I	ACTGG	1 cut(s)	1229
Bse8I	GATNNNNATC	1 cut(s)	663
BseAI	TCCGGA	1 cut(s)	374
BseBI	CCWGG	2 cut(s)	276, 648
BseCI	ATCGAT	1 cut(s)	154
BseDI	CCNNGG	4 cut(s)	360, 647, 876, 1115
BseGI	GGATG	4 cut(s)	502, 664, 1006, 1292
BseJI	GATNNNNATC	1 cut(s)	663
BseMII	CTCAG	3 cut(s)	242, 395, 1175
BseNI	ACTGG	1 cut(s)	1229
BseRI	GAGGAG	3 cut(s)	207, 934, 1347
BseSI	GKGCMC	1 cut(s)	957
BseXI	GCAGC	5 cut(s)	62, 139, 533, 1107, 1107
BseYI	CCCAGC	2 cut(s)	179, 1172
BsgI	GTGCAG	1 cut(s)	510
Bsh1236I	CGCG	1 cut(s)	107
BshFI	GGCC	3 cut(s)	819, 955, 1348
BshVI	ATCGAT	1 cut(s)	154
BsiHKAI	GWGCWC	2 cut(s)	22, 1145
BsiHKCI	CYCGRG	1 cut(s)	15
BsiSI	CCGG	5 cut(s)	6, 41, 162, 375, 423
BslFI	GGGAC	5 cut(s)	197, 285, 869, 1309, 1364
BsmAI	GTCTC	1 cut(s)	593
BsmFI	GGGAC	5 cut(s)	197, 285, 869, 1309, 1364
BsnI	GGCC	3 cut(s)	819, 955, 1348
Bso31I	GGTCTC	1 cut(s)	593
BsoBI	CYCGRG	1 cut(s)	15
Bsp120I	GGGCCC	1 cut(s)	953
Bsp1286I	GDGCHC	3 cut(s)	22, 957, 1145
Bsp13I	TCCGGA	1 cut(s)	374
Bsp143I	GATC	6 cut(s)	212, 371, 508, 664, 724, 889
BspANI	GGCC	3 cut(s)	819, 955, 1348
BspCNI	CTCAG	3 cut(s)	241, 394, 1174
BspDI	ATCGAT	1 cut(s)	154
BspEI	TCCGGA	1 cut(s)	374
BspFNI	CGCG	1 cut(s)	107
BspLI	GGNNCC	7 cut(s)	73, 185, 406, 871, 954, 955, 1054
BspPI	GGATC	2 cut(s)	366, 884
BspTNI	GGTCTC	1 cut(s)	593
BsrFI	RCCGGY	1 cut(s)	40
BsrI	ACTGG	1 cut(s)	1229
BssAI	RCCGGY	1 cut(s)	40
BssECI	CCNNGG	4 cut(s)	360, 647, 876, 1115
BssMI	GATC	6 cut(s)	212, 371, 508, 664, 724, 889
BssNI	GRCGYC	2 cut(s)	97, 386
BssT1I	CCWWGG	3 cut(s)	360, 876, 1115
Bst2UI	CCWGG	2 cut(s)	276, 648
Bst4CI	ACNGT	3 cut(s)	257, 443, 1087
Bst6I	CTCTTC	1 cut(s)	539
BstACI	GRCGYC	2 cut(s)	97, 386
BstBAI	YACGTR	1 cut(s)	1140
BstC8I	GCNNGC	2 cut(s)	42, 489
BstDEI	CTNAG	4 cut(s)	228, 381, 938, 1161
BstF5I	GGATG	4 cut(s)	502, 664, 1006, 1292
BstFNI	CGCG	1 cut(s)	107
BstHHI	GCGC	1 cut(s)	714
BstKTI	GATC	6 cut(s)	215, 374, 511, 667, 727, 892
BstMAI	GTCTC	1 cut(s)	593
BstMBI	GATC	6 cut(s)	212, 371, 508, 664, 724, 889
BstMWI	GCNNNNNNNGC	9 cut(s)	41, 50, 66, 69, 94, 171, 825, 857, 1120
BstNI	CCWGG	2 cut(s)	276, 648
BstSCI	CCNGG	2 cut(s)	274, 646
BstSLI	GKGCMC	1 cut(s)	957
BstUI	CGCG	1 cut(s)	107
BstV1I	GCAGC	5 cut(s)	62, 139, 533, 1107, 1107
BstX2I	RGATCY	2 cut(s)	371, 889
BstYI	RGATCY	2 cut(s)	371, 889
Bsu15I	ATCGAT	1 cut(s)	154
BsuI	GTATCC	1 cut(s)	609
BsuRI	GGCC	3 cut(s)	819, 955, 1348
BsuTUI	ATCGAT	1 cut(s)	154
BtsCI	GGATG	4 cut(s)	502, 664, 1006, 1292
BtsIMutI	CAGTG	1 cut(s)	262
Cac8I	GCNNGC	2 cut(s)	42, 489
CaiI	CAGNNNCTG	2 cut(s)	82, 893
CfoI	GCGC	1 cut(s)	714
Cfr10I	RCCGGY	1 cut(s)	40
Cfr13I	GGNCC	8 cut(s)	167, 184, 264, 377, 817, 953, 954, 1053
ClaI	ATCGAT	1 cut(s)	154
CseI	GACGC	2 cut(s)	86, 394
Csp6I	GTAC	2 cut(s)	253, 1338
CspCI	CAANNNNNGTGG	2 cut(s)	1059, 1094
CviAII	CATG	5 cut(s)	866, 1007, 1150, 1310, 1388
CviQI	GTAC	2 cut(s)	253, 1338
DdeI	CTNAG	4 cut(s)	228, 381, 938, 1161
DpnI	GATC	6 cut(s)	214, 373, 510, 666, 726, 891
DpnII	GATC	6 cut(s)	212, 371, 508, 664, 724, 889
DrdI	GACNNNNNNGTC	2 cut(s)	316, 391
DriI	GACNNNNNGTC	1 cut(s)	270
DseDI	GACNNNNNNGTC	2 cut(s)	316, 391
Eam1104I	CTCTTC	1 cut(s)	539
Eam1105I	GACNNNNNGTC	1 cut(s)	270
EarI	CTCTTC	1 cut(s)	539
EciI	GGCGGA	1 cut(s)	565
Eco130I	CCWWGG	3 cut(s)	360, 876, 1115
Eco24I	GRGCYC	1 cut(s)	957
Eco31I	GGTCTC	1 cut(s)	593
Eco47I	GGWCC	5 cut(s)	167, 184, 264, 377, 1053
Eco57I	CTGAAG	4 cut(s)	216, 290, 626, 822
Eco72I	CACGTG	1 cut(s)	1140
Eco88I	CYCGRG	1 cut(s)	15
EcoO109I	RGGNCCY	2 cut(s)	817, 953
EcoRI	GAATTC	1 cut(s)	501
EcoRII	CCWGG	2 cut(s)	274, 646
EcoT14I	CCWWGG	3 cut(s)	360, 876, 1115
EcoT38I	GRGCYC	1 cut(s)	957
ErhI	CCWWGG	3 cut(s)	360, 876, 1115
FaeI	CATG	5 cut(s)	869, 1010, 1153, 1313, 1391
FalI	AAGNNNNNCTT	2 cut(s)	189, 221
FaqI	GGGAC	5 cut(s)	197, 285, 869, 1309, 1364
FatI	CATG	5 cut(s)	865, 1006, 1149, 1309, 1387
FauI	CCCGC	1 cut(s)	1091
FblI	GTMKAC	1 cut(s)	966
FokI	GGATG	4 cut(s)	509, 671, 1013, 1299
FriOI	GRGCYC	1 cut(s)	957
FspBI	CTAG	2 cut(s)	449, 1365
GlaI	GCGC	1 cut(s)	713
GsaI	CCCAGC	2 cut(s)	183, 1176
GsuI	CTGGAG	1 cut(s)	402
HaeIII	GGCC	3 cut(s)	819, 955, 1348
HapII	CCGG	5 cut(s)	6, 41, 162, 375, 423
HgaI	GACGC	2 cut(s)	86, 394
HhaI	GCGC	1 cut(s)	714
Hin1I	GRCGYC	2 cut(s)	97, 386
Hin1II	CATG	5 cut(s)	869, 1010, 1153, 1313, 1391
Hin6I	GCGC	1 cut(s)	712
HinP1I	GCGC	1 cut(s)	712
HincII	GTYRAC	3 cut(s)	459, 907, 1396
HindII	GTYRAC	3 cut(s)	459, 907, 1396
HinfI	GANTC	6 cut(s)	79, 113, 156, 298, 310, 1274
HpaI	GTTAAC	1 cut(s)	1396
HpaII	CCGG	5 cut(s)	6, 41, 162, 375, 423
HphI	GGTGA	2 cut(s)	92, 674
Hpy166II	GTNNAC	7 cut(s)	117, 340, 459, 907, 967, 1027, 1396
Hpy188I	TCNGA	7 cut(s)	231, 309, 384, 543, 1147, 1201, 1237
Hpy188III	TCNNGA	5 cut(s)	140, 375, 506, 893, 1163
Hpy8I	GTNNAC	7 cut(s)	117, 340, 459, 907, 967, 1027, 1396
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	8 cut(s)	226, 314, 327, 420, 572, 619, 1096, 1206
HpyCH4III	ACNGT	3 cut(s)	257, 443, 1087
HpyCH4IV	ACGT	2 cut(s)	619, 1139
HpyCH4V	TGCA	6 cut(s)	323, 491, 742, 1006, 1123, 1217
HpyF10VI	GCNNNNNNNGC	9 cut(s)	41, 50, 66, 69, 94, 171, 825, 857, 1120
HpyF3I	CTNAG	4 cut(s)	228, 381, 938, 1161
HpySE526I	ACGT	2 cut(s)	619, 1139
Hsp92I	GRCGYC	2 cut(s)	97, 386
Hsp92II	CATG	5 cut(s)	869, 1010, 1153, 1313, 1391
HspAI	GCGC	1 cut(s)	712
Kpn2I	TCCGGA	1 cut(s)	374
KroI	GCCGGC	1 cut(s)	40
KroNI	GCCGGC	1 cut(s)	42
KspAI	GTTAAC	1 cut(s)	1396
Kzo9I	GATC	6 cut(s)	212, 371, 508, 664, 724, 889
LmnI	GCTCC	2 cut(s)	77, 865
Lsp1109I	GCAGC	5 cut(s)	62, 139, 533, 1107, 1107
LweI	GCATC	4 cut(s)	625, 729, 1204, 1228
MaeI	CTAG	2 cut(s)	449, 1365
MaeII	ACGT	2 cut(s)	619, 1139
MaeIII	GTNAC	4 cut(s)	98, 796, 1081, 1324
MalI	GATC	6 cut(s)	214, 373, 510, 666, 726, 891
MboI	GATC	6 cut(s)	212, 371, 508, 664, 724, 889
MboII	GAAGA	5 cut(s)	556, 619, 815, 1198, 1298
MfeI	CAATTG	2 cut(s)	396, 1035
MflI	RGATCY	2 cut(s)	371, 889
MhlI	GDGCHC	3 cut(s)	22, 957, 1145
MluCI	AATT	8 cut(s)	145, 396, 475, 501, 852, 980, 1035, 1107
MlyI	GAGTC	3 cut(s)	122, 304, 307
MmeI	TCCRAC	4 cut(s)	602, 636, 1080, 1264
MreI	CGCCGGCG	1 cut(s)	40
MroI	TCCGGA	1 cut(s)	374
MroNI	GCCGGC	1 cut(s)	40
MroXI	GAANNNNTTC	1 cut(s)	201
MseI	TTAA	4 cut(s)	483, 813, 855, 1395
MspA1I	CMGCKG	1 cut(s)	1098
MspI	CCGG	5 cut(s)	6, 41, 162, 375, 423
MspR9I	CCNGG	2 cut(s)	276, 648
MunI	CAATTG	2 cut(s)	396, 1035
MvaI	CCWGG	2 cut(s)	276, 648
MvnI	CGCG	1 cut(s)	107
MwoI	GCNNNNNNNGC	9 cut(s)	41, 50, 66, 69, 94, 171, 825, 857, 1120
NaeI	GCCGGC	1 cut(s)	42
NdeII	GATC	6 cut(s)	212, 371, 508, 664, 724, 889
NgoMIV	GCCGGC	1 cut(s)	40
NlaIII	CATG	5 cut(s)	869, 1010, 1153, 1313, 1391
NlaIV	GGNNCC	7 cut(s)	73, 185, 406, 871, 954, 955, 1054
NmuCI	GTSAC	3 cut(s)	98, 796, 1324
PaeR7I	CTCGAG	1 cut(s)	15
PdiI	GCCGGC	1 cut(s)	42
PdmI	GAANNNNTTC	1 cut(s)	201
PfeI	GAWTC	3 cut(s)	79, 156, 1274
PfoI	TCCNGGA	1 cut(s)	274
PleI	GAGTC	3 cut(s)	121, 304, 306
PmaCI	CACGTG	1 cut(s)	1140
PmlI	CACGTG	1 cut(s)	1140
PpsI	GAGTC	3 cut(s)	121, 304, 306
Ppu21I	YACGTR	1 cut(s)	1140
Psp6I	CCWGG	2 cut(s)	274, 646
PspCI	CACGTG	1 cut(s)	1140
PspFI	CCCAGC	2 cut(s)	179, 1172
PspGI	CCWGG	2 cut(s)	274, 646
PspN4I	GGNNCC	7 cut(s)	73, 185, 406, 871, 954, 955, 1054
PspOMI	GGGCCC	1 cut(s)	953
PspPI	GGNCC	8 cut(s)	167, 184, 264, 377, 817, 953, 954, 1053
PspXI	VCTCGAGB	1 cut(s)	15
PstNI	CAGNNNCTG	2 cut(s)	82, 893
PsuI	RGATCY	2 cut(s)	371, 889
RsaI	GTAC	2 cut(s)	254, 1339
RsaNI	GTAC	2 cut(s)	253, 1338
SaqAI	TTAA	4 cut(s)	483, 813, 855, 1395
Sau3AI	GATC	6 cut(s)	212, 371, 508, 664, 724, 889
Sau96I	GGNCC	8 cut(s)	167, 184, 264, 377, 817, 953, 954, 1053
ScaI	AGTACT	1 cut(s)	1339
SchI	GAGTC	3 cut(s)	122, 304, 307
ScrFI	CCNGG	2 cut(s)	276, 648
SduI	GDGCHC	3 cut(s)	22, 957, 1145
SfaNI	GCATC	4 cut(s)	625, 729, 1204, 1228
Sfr274I	CTCGAG	1 cut(s)	15
SgrAI	CRCCGGYG	1 cut(s)	40
SinI	GGWCC	5 cut(s)	167, 184, 264, 377, 1053
SlaI	CTCGAG	1 cut(s)	15
SmlI	CTYRAG	1 cut(s)	15
SmoI	CTYRAG	1 cut(s)	15
Sse9I	AATT	8 cut(s)	145, 396, 475, 501, 852, 980, 1035, 1107
SspMI	CTAG	2 cut(s)	449, 1365
StyD4I	CCNGG	2 cut(s)	274, 646
StyI	CCWWGG	3 cut(s)	360, 876, 1115
TaaI	ACNGT	3 cut(s)	257, 443, 1087
TaiI	ACGT	2 cut(s)	622, 1142
TaqI	TCGA	6 cut(s)	16, 111, 154, 211, 727, 996
TasI	AATT	8 cut(s)	145, 396, 475, 501, 852, 980, 1035, 1107
TatI	WGTACW	1 cut(s)	1337
TauI	GCSGC	7 cut(s)	63, 66, 69, 72, 88, 127, 1351
TfiI	GAWTC	3 cut(s)	79, 156, 1274
Tru1I	TTAA	4 cut(s)	483, 813, 855, 1395
Tru9I	TTAA	4 cut(s)	483, 813, 855, 1395
TscAI	CASTG	1 cut(s)	262
TseFI	GTSAC	3 cut(s)	98, 796, 1324
TseI	GCWGC	5 cut(s)	50, 127, 521, 1095, 1120
Tsp45I	GTSAC	3 cut(s)	98, 796, 1324
TspDTI	ATGAA	9 cut(s)	258, 460, 786, 852, 912, 1121, 1166, 1199, 1298
TspGWI	ACGGA	2 cut(s)	1286, 1313
TspRI	CASTG	1 cut(s)	262
VpaK11BI	GGWCC	5 cut(s)	167, 184, 264, 377, 1053
XapI	RAATTY	1 cut(s)	501
XhoI	CTCGAG	1 cut(s)	15
XmiI	GTMKAC	1 cut(s)	966
XmnI	GAANNNNTTC	1 cut(s)	201
XspI	CTAG	2 cut(s)	449, 1365
ZrmI	AGTACT	1 cut(s)	1339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.