MD12G1054400.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018458
Physical Location & Seq
Reverse (-)
6169088 .. 6169831
744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1054400.v1.1.491

Sequence Viewer

Length: 744 bp
ATGTTCAACAATTCCAAATCCTCAGCTCACCGCTCTAACATCGCTACATGTAACCGTAGCCCTAGCATTGCTCCATGTAACCCTATCACCTCAGATACCTCAATTCCAATGATGTACCCCATGTCACCAATCCTTGGTTCATGTCTCTATTTTCAAAATCGTGGTTCCTGGAAGTACGCATGTAGTAGTTGCGACAAGGCCTTCCCAACAACTCAGGCTTTAGGAGGCCACCAGAACGCTCACCGGAAAGAGCGAAACGATCTCCGTGTGAATGAGGTTGCGCGCACTGCTCAGGAAAATCGCAGGTTCAGACCCTACGTTTTCCAACATGACCCCTGGAGATGGGCACCGCCATCTATCTACAACGATTTCATTAACAAAACGAAGCCTCGAATGCGTTCCTACGATTTCTACCATTTCCTGGGAATGGCGGGGGATTGTAATCCAAAATGGGGTGCAGGGTTAGGGTTTGGATCAGGTTCTAAAAGAAAGAAGCGTGGTTATGATGACAACGACGATGCCGGGGCGGATCGTGACAAGAAGAAGGGTAAGTATGTTGGTGTCATTGATGATTTGAAAGTGTCTATGTCTAATGTGACTCTGAGCTTGGCAACAGGAAGTGGTGACGTGGACAAGTCCGATTCTGGTTTAGATTGGGAGGACGGGGAGTGTATGTACGTTGGTGGTGACGGTGAAGCAAGTACTTCCAAGACTGAAGAACTGGACTTAAATCTGAAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2_6

Protein Analysis

248

Amino Acids

27.53

Weight (kDa)

8.59

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 59 - 84 8.5e-08 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018458)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g21782
malus_domestica MD04G1139700.v1.1 MD12G1054400.v1.1 MD14G1053400.v1.1
prunus_persica Prupe.7G082300_v2.0.a1
rosa_laevigata RLG00000023601
rosa_multiflora Rmu_sc0012914.1_g000004
rosa_roxburghii Rroxscaffold_6G00402610
rosa_rugosa Rorug03G0171600
rosa_samantha Rh3DG247600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 294
AccB1I GGYRCC 1 cut(s) 346
AccB7I CCANNNNNTGG 2 cut(s) 134, 421
AccBSI CCGCTC 1 cut(s) 33
AccII CGCG 1 cut(s) 283
AciI CCGC 4 cut(s) 31, 350, 431, 527
AclWI GGATC 2 cut(s) 481, 537
AcuI CTGAAG 1 cut(s) 735
AfaI GTAC 4 cut(s) 116, 176, 677, 703
AfiI CCNNNNNNNGG 5 cut(s) 134, 342, 421, 427, 452
AflIII ACRYGT 1 cut(s) 47
AgsI TTSAA 3 cut(s) 7, 155, 577
AjiI CACGTC 1 cut(s) 628
AjnI CCWGG 3 cut(s) 167, 335, 420
AjuI GAANNNNNNNTTGG 2 cut(s) 121, 153
AluBI AGCT 2 cut(s) 26, 606
AluI AGCT 2 cut(s) 26, 606
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 2 cut(s) 481, 537
AoxI GGCC 2 cut(s) 198, 226
Asp700I GAANNNNTTC 1 cut(s) 397
AspLEI GCGC 2 cut(s) 283, 285
AsuC2I CCSGG 1 cut(s) 523
AsuHPI GGTGA 7 cut(s) 20, 79, 117, 233, 635, 698, 704
BaeGI GKGCMC 1 cut(s) 349
BanI GGYRCC 1 cut(s) 346
BbvCI CCTCAGC 1 cut(s) 22
BccI CCATC 2 cut(s) 336, 361
BciT130I CCWGG 3 cut(s) 169, 337, 422
BcnI CCSGG 1 cut(s) 523
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 1 cut(s) 63
BfuAI ACCTGC 1 cut(s) 294
BmcAI AGTACT 1 cut(s) 703
Bme1390I CCNGG 4 cut(s) 169, 337, 422, 523
BmgBI CACGTC 1 cut(s) 628
BmiI GGNNCC 2 cut(s) 166, 348
BmrFI CCNGG 4 cut(s) 169, 337, 422, 523
BmsI GCATC 1 cut(s) 508
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 2 cut(s) 22, 291
BpuMI CCSGG 1 cut(s) 523
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 4 cut(s) 133, 335, 421, 522
BsaWI WCCGGW 1 cut(s) 243
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 5 cut(s) 134, 342, 421, 427, 452
Bse1I ACTGG 1 cut(s) 726
Bse3DI GCAATG 1 cut(s) 66
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 3 cut(s) 169, 337, 422
BseDI CCNNGG 4 cut(s) 133, 335, 421, 522
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 5 cut(s) 134, 342, 421, 427, 452
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 5 cut(s) 36, 105, 227, 305, 593
BseNI ACTGG 1 cut(s) 726
BsePI GCGCGC 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 349
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 2 cut(s) 200, 228
BshNI GGYRCC 1 cut(s) 346
BsiSI CCGG 2 cut(s) 244, 522
BslI CCNNNNNNNGG 5 cut(s) 134, 342, 421, 427, 452
BsmAI GTCTC 1 cut(s) 149
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 2 cut(s) 200, 228
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 3 cut(s) 259, 473, 529
BspACI CCGC 4 cut(s) 31, 350, 431, 527
BspANI GGCC 2 cut(s) 200, 228
BspCNI CTCAG 5 cut(s) 35, 104, 226, 304, 594
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 166, 348
BspMI ACCTGC 1 cut(s) 294
BspPI GGATC 2 cut(s) 481, 537
BspT107I GGYRCC 1 cut(s) 346
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 66
BsrI ACTGG 1 cut(s) 726
BssECI CCNNGG 4 cut(s) 133, 335, 421, 522
BssHII GCGCGC 1 cut(s) 281
BssMI GATC 3 cut(s) 259, 473, 529
BssT1I CCWWGG 1 cut(s) 133
Bst2UI CCWGG 3 cut(s) 169, 337, 422
Bst4CI ACNGT 2 cut(s) 56, 692
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 5 cut(s) 22, 91, 213, 291, 602
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 2 cut(s) 283, 285
BstKTI GATC 3 cut(s) 262, 476, 532
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 3 cut(s) 259, 473, 529
BstMWI GCNNNNNNNGC 2 cut(s) 287, 394
BstNI CCWGG 3 cut(s) 169, 337, 422
BstNSI RCATGY 2 cut(s) 51, 183
BstSCI CCNGG 4 cut(s) 167, 335, 420, 521
BstSLI GKGCMC 1 cut(s) 349
BstUI CGCG 1 cut(s) 283
BsuRI GGCC 2 cut(s) 200, 228
BtgZI GCGATG 1 cut(s) 25
BtrI CACGTC 1 cut(s) 628
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 285
BveI ACCTGC 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 283
CfoI GCGC 2 cut(s) 283, 285
Csp6I GTAC 4 cut(s) 115, 175, 676, 702
CviAII CATG 6 cut(s) 48, 75, 121, 141, 180, 329
CviJI RGCY 7 cut(s) 26, 60, 200, 218, 228, 388, 606
CviKI_1 RGCY 7 cut(s) 26, 60, 200, 218, 228, 388, 606
CviQI GTAC 4 cut(s) 115, 175, 676, 702
DdeI CTNAG 5 cut(s) 22, 91, 213, 291, 602
DpnI GATC 3 cut(s) 261, 475, 531
DpnII GATC 3 cut(s) 259, 473, 529
EciI GGCGGA 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 133
Eco147I AGGCCT 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 735
EcoRII CCWGG 3 cut(s) 167, 335, 420
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 6 cut(s) 51, 78, 124, 144, 183, 332
FatI CATG 6 cut(s) 47, 74, 120, 140, 179, 328
FauI CCCGC 1 cut(s) 424
FspBI CTAG 1 cut(s) 63
GlaI GCGC 2 cut(s) 282, 284
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 2 cut(s) 200, 228
HapII CCGG 2 cut(s) 244, 522
HhaI GCGC 2 cut(s) 283, 285
Hin1II CATG 6 cut(s) 51, 78, 124, 144, 183, 332
Hin6I GCGC 2 cut(s) 281, 283
HinP1I GCGC 2 cut(s) 281, 283
HinfI GANTC 2 cut(s) 598, 641
HpaII CCGG 2 cut(s) 244, 522
HphI GGTGA 7 cut(s) 20, 79, 117, 233, 635, 698, 704
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 5 cut(s) 94, 311, 603, 640, 735
Hpy188III TCNNGA 2 cut(s) 293, 533
Hpy8I GTNNAC 1 cut(s) 631
Hpy99I CGWCG 1 cut(s) 518
HpyAV CCTTC 2 cut(s) 211, 538
HpyCH4III ACNGT 2 cut(s) 56, 692
HpyCH4IV ACGT 3 cut(s) 318, 627, 678
HpyCH4V TGCA 1 cut(s) 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 394
HpyF3I CTNAG 5 cut(s) 22, 91, 213, 291, 602
HpySE526I ACGT 3 cut(s) 318, 627, 678
Hsp92II CATG 6 cut(s) 51, 78, 124, 144, 183, 332
HspAI GCGC 2 cut(s) 281, 283
Kzo9I GATC 3 cut(s) 259, 473, 529
LmnI GCTCC 1 cut(s) 76
LweI GCATC 1 cut(s) 508
MaeI CTAG 1 cut(s) 63
MaeII ACGT 3 cut(s) 318, 627, 678
MaeIII GTNAC 7 cut(s) 50, 77, 123, 533, 595, 623, 686
MalI GATC 3 cut(s) 261, 475, 531
MbiI CCGCTC 1 cut(s) 33
MboI GATC 3 cut(s) 259, 473, 529
MboII GAAGA 2 cut(s) 553, 728
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 2 cut(s) 10, 102
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 7 cut(s) 31, 100, 109, 218, 268, 399, 652
MroXI GAANNNNTTC 1 cut(s) 397
MseI TTAA 2 cut(s) 375, 728
MspI CCGG 2 cut(s) 244, 522
MspR9I CCNGG 4 cut(s) 169, 337, 422, 523
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 3 cut(s) 169, 337, 422
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 2 cut(s) 287, 394
NciI CCSGG 1 cut(s) 523
NdeII GATC 3 cut(s) 259, 473, 529
NlaIII CATG 6 cut(s) 51, 78, 124, 144, 183, 332
NlaIV GGNNCC 2 cut(s) 166, 348
NmuCI GTSAC 5 cut(s) 123, 533, 595, 623, 686
NspI RCATGY 2 cut(s) 51, 183
PauI GCGCGC 1 cut(s) 281
PceI AGGCCT 1 cut(s) 200
PciI ACATGT 1 cut(s) 47
PctI GAATGC 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 397
PfeI GAWTC 1 cut(s) 641
PflMI CCANNNNNTGG 2 cut(s) 134, 421
PfoI TCCNGGA 1 cut(s) 167
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PscI ACATGT 1 cut(s) 47
Psp6I CCWGG 3 cut(s) 167, 335, 420
PspGI CCWGG 3 cut(s) 167, 335, 420
PspN4I GGNNCC 2 cut(s) 166, 348
PteI GCGCGC 1 cut(s) 281
RsaI GTAC 4 cut(s) 116, 176, 677, 703
RsaNI GTAC 4 cut(s) 115, 175, 676, 702
SaqAI TTAA 2 cut(s) 375, 728
Sau3AI GATC 3 cut(s) 259, 473, 529
ScaI AGTACT 1 cut(s) 703
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 4 cut(s) 169, 337, 422, 523
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 1 cut(s) 508
Sse9I AATT 2 cut(s) 10, 102
SseBI AGGCCT 1 cut(s) 200
SsiI CCGC 4 cut(s) 31, 350, 431, 527
SspMI CTAG 1 cut(s) 63
StuI AGGCCT 1 cut(s) 200
StyD4I CCNGG 4 cut(s) 167, 335, 420, 521
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 56, 692
TaiI ACGT 3 cut(s) 321, 630, 681
TaqI TCGA 1 cut(s) 391
TasI AATT 2 cut(s) 10, 102
TatI WGTACW 1 cut(s) 701
TfiI GAWTC 1 cut(s) 641
Tru1I TTAA 2 cut(s) 375, 728
Tru9I TTAA 2 cut(s) 375, 728
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 5 cut(s) 123, 533, 595, 623, 686
Tsp45I GTSAC 5 cut(s) 123, 533, 595, 623, 686
TspDTI ATGAA 2 cut(s) 129, 361
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 1 cut(s) 292
Van91I CCANNNNNTGG 2 cut(s) 134, 421
XceI RCATGY 2 cut(s) 51, 183
XmnI GAANNNNTTC 1 cut(s) 397
XspI CTAG 1 cut(s) 63
ZrmI AGTACT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.