Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003863

Physical Location & Seq

Genomic Coordinates Forward (+)

7666314 .. 7672190

Gene Structure 5877 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1065800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGGAAGATTATGAAGTAATAGAGCAGATTGGGAGAGGGGCTTTTGGAGCCGCCTTTCTTGTTCTTCATAAGACTCAGAAAAAGAAGTATGTATTGAAAAAGATTCGTTTGACTAAGCAAGCGGAGAAGTTCAAACGCACAGCTCACCAAGAGATGAATTTAATTGCAAAGTTGAATCATCCATATATTGTGGATTATAAAGATGCTTGGGTGGACAAGGGAGACTGCATATGTATTGTAACGGGCTATTGTGAAGGAGGTGACATGGCTGATATGGTAAAGAAGGCCAGAGGACAATTTTTTCCAGAGGAGAAGCTCTGCAAATGGCTGACTCAGTTGTTGCTAGCTGTGGACTACTTGCACTCCAATCGTGTACTTCATAGGGATCTTAAGTGCTCAAATGTATTCCTTACAAAGGACAATGACATCCGACTTGGTGACTTTGGGCTTGCAAAACTACTCAACACAGAAGATCTTACTTCTTCGGTTGTCGGAACTCCAAACTACATGTGTCCTGAGCTCCTTGCAGATATACCTTATGGCTATAAATCTGACATATGGTCGCTTGGTTGCTGTATGTTTGAGATTGCCGCACATCAACCTGCATTTAGAGCGCCTGACATGGCTGGGCTTATCAACAAAATTAACAGATCCACCATTTCTCCTCTTCCAATTGTGTATTCTTCCACACTGAAACAAATCATCAAGAGCATGCTCAGGAAAAGTCCAGAACACAGACCAACAGCTGCAGAGTTATTAAGGCATCCACACTTACAACCATACGTCCTTCAATGCCGCAATGCATCATCTGTTTTTCTTCCTGTATGTCCACAAACCAACTTAAAAGAAAAAACTCCAAGAAAATCACCACCTAGCAAGCGCAGCAGTGGCAAAGACAACAGAGATAGAGAGGCTGGAAAAATGAACCACGTGGAAAAGGTCCAACCATTTGGGAGCAATGCTGATCTGACGCATAGATATGTAACAAACATTGATAACCCTGTATTCACAGCTAGTGCAGAAGAGCTTGAAACCAAGAGAGTTGATCCTACTAGCTGTTCTTTGGAAGTATCCAATGCCACTGATGGTTCAAAAGATGGGCCGACTGATTCAGAAGCATCTGTTTGCGATAAGCAAGCTGACACTATCAGTATAGCACAAAAGGAAAATACTGAATCTGACATTGAGGTTACATCAGAGAGCGCATCAAATTCTCAGCATGAAGAACTAGAAGAACCAACTGCCTCAAATTTCAAACACTTGAAAGAGGTTGATTTCAAGACTCTGAACAGTGAGGACAGAAAGGCATATGAAAATCAACAAGTTCTTGAAGGAGCTAAAGCAGAAGGAGAGGGGGCTAATGAAGAAAATTGCAGGGTATTGGCGGTGCCCAGTGTAAGCGGTACTGAAAAAGTTGGGTGGATTGATGATACTGAAGTGGAACCCAGTTGTTGTTTAGAGAAGGAAAGGTCCAATGTGTATACTGAAGTTGGTAATGCGGAATACTTGTTATCTGAAAATAACAATGAAAGCTCCATACAGAAAGCAAAACATGAAGTGGTGGGAAAGGCAGACAATAACAATTGCTCTGCGCAGATAGAAAAAGGTGAGGCTCGTGTGATTAATCAAGCAGCAGGTGATGTAGCGGCAGTAGGCAGTGATGATTCCAAGAGTGACTGGGAAAATCCAAGTCAGCAAAGAGCTGATGCCCTTGAGTCTCTGCTTGAGCTATGCGCACGGCTACTAAAGCAGGACAAACTTGATGAGCTTGCCGGCGTGCTAAGACCATTTGGAGAAGACGCCGTCTCTTCTAGAGAAACAGCTATCTGGTTGACAAAGAGTCTCATGTCTGCACAGAAATCTAATGGAGAATCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

626

Amino Acids

69.39

Weight (kDa)

5.42

Isoelectric Point (pI)

36.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	4 - 257	9e-68	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	6 - 254	3.1e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20860
fragaria_vesca	FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080
malus_domestica	MD12G1065800.v1.1 MD14G1065800.v1.1
prunus_persica	Prupe.7G068700_v2.0.a1
pyrus_communis	pycom12g05710 pycom14g05320
rosa_chinensis	RchiOBHm_Chr3g0480931
rosa_laevigata	RLG00000023440
rosa_multiflora	Rmu_sc0006028.1_g000015 Rmu_sc0006048.1_g000005
rosa_roxburghii	Rroxscaffold_6G00400870
rosa_rugosa	Rorug03G0187700 Rorug03G0187800 Rorug03G0187900 Rorug03G0188000
rosa_samantha	Rh3AG239200 Rh3BG273400 Rh3CG269000 Rh3DG265000
rosa_wichuraiana	Rw3G021400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	198
AarI	CACCTGC	1 cut(s)	1625
Acc16I	TGCGCA	2 cut(s)	1593, 1735
Acc36I	ACCTGC	2 cut(s)	610, 1625
AccB1I	GGYRCC	1 cut(s)	1387
AccI	GTMKAC	1 cut(s)	1481
AciI	CCGC	8 cut(s)	51, 122, 591, 796, 1385, 1401, 1499, 1646
AclWI	GGATC	3 cut(s)	393, 645, 1040
AcsI	RAATTY	3 cut(s)	157, 1210, 1249
AcuI	CTGAAG	2 cut(s)	1455, 1506
AcvI	CACGTG	1 cut(s)	931
AcyI	GRCGYC	1 cut(s)	1800
AfaI	GTAC	2 cut(s)	375, 1405
AfiI	CCNNNNNNNGG	1 cut(s)	415
AflII	CTTAAG	1 cut(s)	389
AflIII	ACRYGT	1 cut(s)	507
AhdI	GACNNNNNGTC	2 cut(s)	559, 1839
AjuI	GAANNNNNNNTTGG	2 cut(s)	664, 696
Alw21I	GWGCWC	2 cut(s)	398, 522
Alw26I	GTCTC	4 cut(s)	216, 1722, 1810, 1847
AlwI	GGATC	3 cut(s)	393, 645, 1040
AoxI	GGCC	2 cut(s)	285, 1100
ApeKI	GCWGC	3 cut(s)	746, 882, 1631
ApoI	RAATTY	3 cut(s)	157, 1210, 1249
AseI	ATTAAT	1 cut(s)	1623
AspLEI	GCGC	5 cut(s)	616, 882, 1205, 1594, 1736
AspS9I	GGNCC	3 cut(s)	940, 1100, 1470
AsuHPI	GGTGA	6 cut(s)	137, 272, 449, 858, 1619, 1649
AsuNHI	GCTAGC	1 cut(s)	343
AvaII	GGWCC	2 cut(s)	940, 1470
BaeGI	GKGCMC	1 cut(s)	1392
BaeI	ACNNNNGTAYC	2 cut(s)	1422, 1455
BanI	GGYRCC	1 cut(s)	1387
BanII	GRGCYC	1 cut(s)	522
BauI	CACGAG	1 cut(s)	1614
BbrPI	CACGTG	1 cut(s)	931
BbsI	GAAGAC	1 cut(s)	1803
Bbv12I	GWGCWC	2 cut(s)	398, 522
BbvI	GCAGC	3 cut(s)	733, 894, 1643
BccI	CCATC	2 cut(s)	1079, 1091
BceAI	ACGGC	2 cut(s)	1754, 1787
BcgI	CGANNNNNNTGC	2 cut(s)	350, 384
BciVI	GTATCC	1 cut(s)	1081
BcoDI	GTCTC	4 cut(s)	216, 1722, 1810, 1847
BfaI	CTAG	6 cut(s)	344, 873, 1014, 1053, 1229, 1812
BfmI	CTRYAG	1 cut(s)	747
BfoI	RGCGCY	1 cut(s)	617
BfrI	CTTAAG	1 cut(s)	389
BfuAI	ACCTGC	2 cut(s)	610, 1625
BfuI	GTATCC	1 cut(s)	1081
BglII	AGATCT	1 cut(s)	472
BisI	GCNGC	7 cut(s)	51, 591, 747, 796, 883, 1632, 1647
BlsI	GCNGC	7 cut(s)	52, 592, 748, 797, 884, 1633, 1648
Bme18I	GGWCC	2 cut(s)	940, 1470
BmeRI	GACNNNNNGTC	2 cut(s)	559, 1839
BmgT120I	GGNCC	3 cut(s)	940, 1100, 1470
BmiI	GGNNCC	3 cut(s)	49, 1389, 1443
BmrI	ACTGGG	3 cut(s)	1386, 1440, 1687
BmsI	GCATC	6 cut(s)	193, 772, 812, 1127, 1214, 1696
BmtI	GCTAGC	1 cut(s)	347
BmuI	ACTGGG	3 cut(s)	1386, 1440, 1687
BpiI	GAAGAC	1 cut(s)	1803
Bpu10I	CCTNAGC	2 cut(s)	516, 716
BpuEI	CTTGAG	2 cut(s)	1733, 1745
BsaAI	YACGTR	1 cut(s)	931
BsaHI	GRCGYC	1 cut(s)	1800
BsaXI	ACNNNNNCTCC	4 cut(s)	347, 377, 646, 676
Bsc4I	CCNNNNNNNGG	1 cut(s)	415
Bse118I	RCCGGY	1 cut(s)	1772
Bse1I	ACTGG	3 cut(s)	1392, 1446, 1682
Bse3DI	GCAATG	2 cut(s)	805, 964
BseGI	GGATG	3 cut(s)	178, 426, 763
BseLI	CCNNNNNNNGG	1 cut(s)	415
BseMI	GCAATG	2 cut(s)	805, 964
BseMII	CTCAG	5 cut(s)	89, 347, 507, 730, 1229
BseNI	ACTGG	3 cut(s)	1392, 1446, 1682
BseRI	GAGGAG	2 cut(s)	323, 654
BseSI	GKGCMC	1 cut(s)	1392
BseXI	GCAGC	3 cut(s)	733, 894, 1643
BseYI	CCCAGC	1 cut(s)	626
BsgI	GTGCAG	2 cut(s)	1038, 1836
BshFI	GGCC	2 cut(s)	287, 1102
BshNI	GGYRCC	1 cut(s)	1387
BsiHKAI	GWGCWC	2 cut(s)	398, 522
BsiSI	CCGG	1 cut(s)	1773
BslI	CCNNNNNNNGG	1 cut(s)	415
BsmAI	GTCTC	4 cut(s)	216, 1722, 1810, 1847
BsmBI	CGTCTC	1 cut(s)	1810
BsnI	GGCC	2 cut(s)	287, 1102
Bsp1286I	GDGCHC	3 cut(s)	398, 522, 1392
Bsp143I	GATC	5 cut(s)	385, 472, 650, 964, 1045
BspACI	CCGC	8 cut(s)	51, 122, 591, 796, 1385, 1401, 1499, 1646
BspANI	GGCC	2 cut(s)	287, 1102
BspCNI	CTCAG	5 cut(s)	88, 346, 508, 729, 1228
BspLI	GGNNCC	3 cut(s)	49, 1389, 1443
BspMAI	CTGCAG	1 cut(s)	751
BspMI	ACCTGC	2 cut(s)	610, 1625
BspOI	GCTAGC	1 cut(s)	347
BspPI	GGATC	3 cut(s)	393, 645, 1040
BspQI	GCTCTTC	1 cut(s)	1017
BspT107I	GGYRCC	1 cut(s)	1387
BspTI	CTTAAG	1 cut(s)	389
BsrDI	GCAATG	2 cut(s)	805, 964
BsrFI	RCCGGY	1 cut(s)	1772
BsrI	ACTGG	3 cut(s)	1392, 1446, 1682
BssAI	RCCGGY	1 cut(s)	1772
BssMI	GATC	5 cut(s)	385, 472, 650, 964, 1045
BssNAI	GTATAC	1 cut(s)	1482
BssNI	GRCGYC	1 cut(s)	1800
BssSI	CACGAG	1 cut(s)	1614
Bst1107I	GTATAC	1 cut(s)	1482
Bst2BI	CACGAG	1 cut(s)	1614
Bst4CI	ACNGT	1 cut(s)	1292
Bst6I	CTCTTC	3 cut(s)	672, 1017, 1813
BstACI	GRCGYC	1 cut(s)	1800
BstAFI	CTTAAG	1 cut(s)	389
BstBAI	YACGTR	1 cut(s)	931
BstC8I	GCNNGC	9 cut(s)	120, 345, 450, 713, 878, 1137, 1770, 1774, 1778
BstDEI	CTNAG	7 cut(s)	75, 114, 333, 516, 716, 1215, 1781
BstENI	CCTNNNNNAGG	1 cut(s)	413
BstF5I	GGATG	3 cut(s)	178, 426, 763
BstH2I	RGCGCY	1 cut(s)	617
BstHHI	GCGC	5 cut(s)	616, 882, 1205, 1594, 1736
BstKTI	GATC	5 cut(s)	388, 475, 653, 967, 1048
BstMAI	GTCTC	4 cut(s)	216, 1722, 1810, 1847
BstMBI	GATC	5 cut(s)	385, 472, 650, 964, 1045
BstMWI	GCNNNNNNNGC	5 cut(s)	47, 611, 882, 888, 1747
BstNSI	RCATGY	2 cut(s)	511, 715
BstSFI	CTRYAG	1 cut(s)	747
BstSLI	GKGCMC	1 cut(s)	1392
BstV1I	GCAGC	3 cut(s)	733, 894, 1643
BstV2I	GAAGAC	1 cut(s)	1803
BstX2I	RGATCY	3 cut(s)	385, 472, 650
BstXI	CCANNNNNNTGG	1 cut(s)	950
BstYI	RGATCY	3 cut(s)	385, 472, 650
BstZ17I	GTATAC	1 cut(s)	1482
BsuI	GTATCC	1 cut(s)	1081
BsuRI	GGCC	2 cut(s)	287, 1102
BtsCI	GGATG	3 cut(s)	178, 426, 763
BtsI	GCAGTG	2 cut(s)	892, 1663
BtsIMutI	CAGTG	6 cut(s)	689, 892, 1080, 1297, 1399, 1663
BveI	ACCTGC	2 cut(s)	610, 1625
Cac8I	GCNNGC	9 cut(s)	120, 345, 450, 713, 878, 1137, 1770, 1774, 1778
CfoI	GCGC	5 cut(s)	616, 882, 1205, 1594, 1736
Cfr10I	RCCGGY	1 cut(s)	1772
Cfr13I	GGNCC	3 cut(s)	940, 1100, 1470
CseI	GACGC	2 cut(s)	979, 1808
Csp6I	GTAC	2 cut(s)	374, 1404
CviAII	CATG	7 cut(s)	265, 508, 622, 712, 1220, 1553, 1846
CviQI	GTAC	2 cut(s)	374, 1404
DdeI	CTNAG	7 cut(s)	75, 114, 333, 516, 716, 1215, 1781
DpnI	GATC	5 cut(s)	387, 474, 652, 966, 1047
DpnII	GATC	5 cut(s)	385, 472, 650, 964, 1045
DriI	GACNNNNNGTC	2 cut(s)	559, 1839
Eam1104I	CTCTTC	3 cut(s)	672, 1017, 1813
Eam1105I	GACNNNNNGTC	2 cut(s)	559, 1839
EarI	CTCTTC	3 cut(s)	672, 1017, 1813
Ecl136II	GAGCTC	1 cut(s)	520
Eco24I	GRGCYC	1 cut(s)	522
Eco47I	GGWCC	2 cut(s)	940, 1470
Eco53kI	GAGCTC	1 cut(s)	520
Eco57I	CTGAAG	2 cut(s)	1455, 1506
Eco72I	CACGTG	1 cut(s)	931
EcoICRI	GAGCTC	1 cut(s)	520
EcoNI	CCTNNNNNAGG	1 cut(s)	413
EcoT22I	ATGCAT	1 cut(s)	805
EcoT38I	GRGCYC	1 cut(s)	522
Esp3I	CGTCTC	1 cut(s)	1810
FaeI	CATG	7 cut(s)	268, 511, 625, 715, 1223, 1556, 1849
FatI	CATG	7 cut(s)	264, 507, 621, 711, 1219, 1552, 1845
FauNDI	CATATG	3 cut(s)	230, 557, 1309
FblI	GTMKAC	1 cut(s)	1481
Fnu4HI	GCNGC	7 cut(s)	51, 591, 747, 796, 883, 1632, 1647
FokI	GGATG	3 cut(s)	165, 413, 750
FriOI	GRGCYC	1 cut(s)	522
Fsp4HI	GCNGC	7 cut(s)	51, 591, 747, 796, 883, 1632, 1647
FspAI	RTGCGCAY	1 cut(s)	1735
FspBI	CTAG	6 cut(s)	344, 873, 1014, 1053, 1229, 1812
FspI	TGCGCA	2 cut(s)	1593, 1735
GlaI	GCGC	5 cut(s)	615, 881, 1204, 1593, 1735
GluI	GCNGC	7 cut(s)	51, 591, 747, 796, 883, 1632, 1647
GsaI	CCCAGC	1 cut(s)	630
HaeII	RGCGCY	1 cut(s)	617
HaeIII	GGCC	2 cut(s)	287, 1102
HapII	CCGG	1 cut(s)	1773
HgaI	GACGC	2 cut(s)	979, 1808
HhaI	GCGC	5 cut(s)	616, 882, 1205, 1594, 1736
Hin1I	GRCGYC	1 cut(s)	1800
Hin1II	CATG	7 cut(s)	268, 511, 625, 715, 1223, 1556, 1849
Hin6I	GCGC	5 cut(s)	614, 880, 1203, 1592, 1734
HinP1I	GCGC	5 cut(s)	614, 880, 1203, 1592, 1734
HincII	GTYRAC	1 cut(s)	1833
HindII	GTYRAC	1 cut(s)	1833
HpaII	CCGG	1 cut(s)	1773
HphI	GGTGA	6 cut(s)	137, 272, 449, 858, 1619, 1649
Hpy166II	GTNNAC	6 cut(s)	214, 352, 374, 830, 1482, 1833
Hpy188III	TCNNGA	8 cut(s)	305, 515, 706, 718, 728, 1279, 1328, 1812
Hpy8I	GTNNAC	6 cut(s)	214, 352, 374, 830, 1482, 1833
HpyAV	CCTTC	6 cut(s)	248, 277, 797, 1325, 1340, 1456
HpyCH4III	ACNGT	1 cut(s)	1292
HpyCH4IV	ACGT	2 cut(s)	783, 930
HpyF10VI	GCNNNNNNNGC	5 cut(s)	47, 611, 882, 888, 1747
HpyF3I	CTNAG	7 cut(s)	75, 114, 333, 516, 716, 1215, 1781
HpySE526I	ACGT	2 cut(s)	783, 930
Hsp92I	GRCGYC	1 cut(s)	1800
Hsp92II	CATG	7 cut(s)	268, 511, 625, 715, 1223, 1556, 1849
HspAI	GCGC	5 cut(s)	614, 880, 1203, 1592, 1734
KroI	GCCGGC	1 cut(s)	1772
KroNI	GCCGGC	1 cut(s)	1774
Kzo9I	GATC	5 cut(s)	385, 472, 650, 964, 1045
LguI	GCTCTTC	1 cut(s)	1017
LmnI	GCTCC	5 cut(s)	47, 525, 954, 1334, 1538
Lsp1109I	GCAGC	3 cut(s)	733, 894, 1643
LweI	GCATC	6 cut(s)	193, 772, 812, 1127, 1214, 1696
MaeI	CTAG	6 cut(s)	344, 873, 1014, 1053, 1229, 1812
MaeII	ACGT	2 cut(s)	783, 930
MaeIII	GTNAC	6 cut(s)	238, 260, 437, 982, 1189, 1673
MalI	GATC	5 cut(s)	387, 474, 652, 966, 1047
MboI	GATC	5 cut(s)	385, 472, 650, 964, 1045
MfeI	CAATTG	2 cut(s)	672, 1582
MflI	RGATCY	3 cut(s)	385, 472, 650
MhlI	GDGCHC	3 cut(s)	398, 522, 1392
MluCI	AATT	9 cut(s)	157, 162, 296, 642, 672, 1210, 1249, 1369, 1582
MlyI	GAGTC	5 cut(s)	67, 325, 1276, 1724, 1849
MmeI	TCCRAC	3 cut(s)	454, 472, 967
Mph1103I	ATGCAT	1 cut(s)	805
MroNI	GCCGGC	1 cut(s)	1772
MseI	TTAA	6 cut(s)	161, 390, 645, 758, 842, 1623
MslI	CAYNNNNRTG	1 cut(s)	978
MspA1I	CMGCKG	1 cut(s)	746
MspCI	CTTAAG	1 cut(s)	389
MspI	CCGG	1 cut(s)	1773
MunI	CAATTG	2 cut(s)	672, 1582
MwoI	GCNNNNNNNGC	5 cut(s)	47, 611, 882, 888, 1747
NaeI	GCCGGC	1 cut(s)	1774
NdeI	CATATG	3 cut(s)	230, 557, 1309
NdeII	GATC	5 cut(s)	385, 472, 650, 964, 1045
NgoMIV	GCCGGC	1 cut(s)	1772
NheI	GCTAGC	1 cut(s)	343
NlaIII	CATG	7 cut(s)	268, 511, 625, 715, 1223, 1556, 1849
NlaIV	GGNNCC	3 cut(s)	49, 1389, 1443
NmuCI	GTSAC	3 cut(s)	260, 437, 1673
NsbI	TGCGCA	2 cut(s)	1593, 1735
NsiI	ATGCAT	1 cut(s)	805
NspI	RCATGY	2 cut(s)	511, 715
PaeI	GCATGC	1 cut(s)	715
PaqCI	CACCTGC	1 cut(s)	1625
PciI	ACATGT	1 cut(s)	507
PciSI	GCTCTTC	1 cut(s)	1017
PdiI	GCCGGC	1 cut(s)	1774
PfeI	GAWTC	6 cut(s)	103, 175, 1109, 1175, 1664, 1871
PflFI	GACNNNGTC	1 cut(s)	1802
PkrI	GCNGC	7 cut(s)	52, 592, 748, 797, 884, 1633, 1648
PleI	GAGTC	5 cut(s)	67, 325, 1276, 1723, 1848
PmaCI	CACGTG	1 cut(s)	931
PmlI	CACGTG	1 cut(s)	931
PpsI	GAGTC	5 cut(s)	67, 325, 1276, 1723, 1848
Ppu21I	YACGTR	1 cut(s)	931
PscI	ACATGT	1 cut(s)	507
PshBI	ATTAAT	1 cut(s)	1623
PsiI	TTATAA	1 cut(s)	198
Psp124BI	GAGCTC	1 cut(s)	522
PspCI	CACGTG	1 cut(s)	931
PspFI	CCCAGC	1 cut(s)	626
PspN4I	GGNNCC	3 cut(s)	49, 1389, 1443
PspPI	GGNCC	3 cut(s)	940, 1100, 1470
PstI	CTGCAG	1 cut(s)	751
PsuI	RGATCY	3 cut(s)	385, 472, 650
PsyI	GACNNNGTC	1 cut(s)	1802
PvuII	CAGCTG	1 cut(s)	746
RsaI	GTAC	2 cut(s)	375, 1405
RsaNI	GTAC	2 cut(s)	374, 1404
RseI	CAYNNNNRTG	1 cut(s)	978
SacI	GAGCTC	1 cut(s)	522
SapI	GCTCTTC	1 cut(s)	1017
SaqAI	TTAA	6 cut(s)	161, 390, 645, 758, 842, 1623
SatI	GCNGC	7 cut(s)	51, 591, 747, 796, 883, 1632, 1647
Sau3AI	GATC	5 cut(s)	385, 472, 650, 964, 1045
Sau96I	GGNCC	3 cut(s)	940, 1100, 1470
SchI	GAGTC	5 cut(s)	67, 325, 1276, 1724, 1849
SduI	GDGCHC	3 cut(s)	398, 522, 1392
SfaNI	GCATC	6 cut(s)	193, 772, 812, 1127, 1214, 1696
SfcI	CTRYAG	1 cut(s)	747
SinI	GGWCC	2 cut(s)	940, 1470
SmiMI	CAYNNNNRTG	1 cut(s)	978
SmlI	CTYRAG	3 cut(s)	389, 1712, 1724
SmoI	CTYRAG	3 cut(s)	389, 1712, 1724
SphI	GCATGC	1 cut(s)	715
Sse9I	AATT	9 cut(s)	157, 162, 296, 642, 672, 1210, 1249, 1369, 1582
SsiI	CCGC	8 cut(s)	51, 122, 591, 796, 1385, 1401, 1499, 1646
SspMI	CTAG	6 cut(s)	344, 873, 1014, 1053, 1229, 1812
SstI	GAGCTC	1 cut(s)	522
TaaI	ACNGT	1 cut(s)	1292
TaiI	ACGT	2 cut(s)	786, 933
TasI	AATT	9 cut(s)	157, 162, 296, 642, 672, 1210, 1249, 1369, 1582
TatI	WGTACW	1 cut(s)	373
TauI	GCSGC	4 cut(s)	53, 593, 798, 1649
TfiI	GAWTC	6 cut(s)	103, 175, 1109, 1175, 1664, 1871
Tru1I	TTAA	6 cut(s)	161, 390, 645, 758, 842, 1623
Tru9I	TTAA	6 cut(s)	161, 390, 645, 758, 842, 1623
TscAI	CASTG	6 cut(s)	696, 892, 1087, 1297, 1399, 1663
TseFI	GTSAC	3 cut(s)	260, 437, 1673
TseI	GCWGC	3 cut(s)	746, 882, 1631
Tsp45I	GTSAC	3 cut(s)	260, 437, 1673
TspRI	CASTG	6 cut(s)	696, 892, 1087, 1297, 1399, 1663
Tth111I	GACNNNGTC	1 cut(s)	1802
Vha464I	CTTAAG	1 cut(s)	389
VpaK11BI	GGWCC	2 cut(s)	940, 1470
VspI	ATTAAT	1 cut(s)	1623
XagI	CCTNNNNNAGG	1 cut(s)	413
XapI	RAATTY	3 cut(s)	157, 1210, 1249
XbaI	TCTAGA	1 cut(s)	1811
XceI	RCATGY	2 cut(s)	511, 715
XmiI	GTMKAC	1 cut(s)	1481
XspI	CTAG	6 cut(s)	344, 873, 1014, 1053, 1229, 1812
Zsp2I	ATGCAT	1 cut(s)	805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.