Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0003863

Physical Location & Seq

Genomic Coordinates Forward (+)

26672153 .. 26680333

Gene Structure 8181 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG273400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGTTTGACATCACAGCTACGCGCTGGAAAACATTCCATGGTGACCCGCTGAGAAGGAAGATCGACCTGATCTCCCATGAACCTGAAAATGCAAGCAAATCTCCAGCACCCAAGACTCCTACTTTGAGGAATTTCACTTGCCAGTTGGAGCTGTACAGAGCTGATTTGTGCTTTGTTTTGGTGTTAGAGCAGATTGGGAGAGGGGCTTTTGGAGCCGCTTTTCTTGTTCTTCATAAGGGACAGAGAAAGAAGTATGTGTTAAAGAAGATTCGTTTATCTAAGCAAACGGAGAAGTTCAAACGCACAGCTCACCAAGAGATGGATTTAATAGCAAAGTTAAATCATCCATATATTGTGGAGTATAAAGATGCTTGGGTGGACAAGGGAGACTGTGTATGCATTGTAACCAGCTATTGCGAAGGAGGTGACATGGCTGAGATGGTAAAGAAGGCCAGAGGACAATTTTTTCCAGAGGAGAAACTTTGCAAATGGTTGACTCAATTGCTGCTAGCTGTTGACTACTTGCACTCTAATCGTGTGATTCATAGAGATGTTAAGTGTTCAAATATATTCCTTACAAAGGATAATGGCATCCGACTTGGTGATTTTGGACTAGCAAAATTACTGAATACAGAAGATCTTACTTCTTCGGTTGTTGGAACTCCAAATTACATGTGTCCTGAGCTCCTTGCAGACATACCTTACGGCTATAAATCTGATATATGGTCACTTGGGTGCTGTATGTTTGAAATTGCTGCACACCAACCTGCATTTAGAGCTCCTGACATGGCTGCGCTCATCAATAAAATTAACCGTTCCTCCATTTCTCCTCTTCCAATTGTGTATTCTTCCACACTGAAACAAATTATCAAGAGCATGCTCAGGAAGAGTCCTGAACATAGACCAACAGCTGCAGAGTTATTAAGGCATCCACACTTACAACCATACCTCCTTCAATGCCGCAATGCATCATCTGTTTTTCTTCCAGTATTTCCTTTAAACAACTTGAAAGATAAAACTACAAGGAAATCACCATCTAGCAAGCCCATTGGTGGCAAGGACAGCCGAGACAAAGAAGCTGCAACACCCAAGCACTTGGAAAAGGTTCATCCATTCGAGACCAGACCTGATGCTATGAAGAGATATTCAATAAACATTGCCAACCCTGTATTCACAGCTAGTACTGAAGAAAAGCTTGAAACCAAGAGAGTTGATCCTAGTAGCTGTACTGTGGAACCATCTAATGCCACTGCTGGTCCAAAAGATGGGCCAATTGATCCACAAGCGTCTATTTGCAATGAAGATAAGCAATCTGAACCTAATATCTACAATGTGGAGTTATCTAATACCACTGATGGATCAAAAGATGGACCAACTTATTCAGAAGCATCTGTCTGCAATGGAGATAAGCAAGCTGATTGTACTAGTCGTGCTAGCTGTAGTCTGGAATTATCTAATACCAGTGATGGCTCAAAAGATGGTCCAACTGATTCAGAAGCATCTGTTTGTAATGCAGATAAGCAAGCTGACGCCATCAGTATACCCCAAAAGGAGACTACCATGTCCGATGTTGAAACTACATCAGAGAGTACCCCAAATCGGAATGAAGAACTAGAAGAACCTGCTAGTACAAATTCCAAACAATTGCAAGAGGTTGCTTGCAAGACTCTGAACTTTGAGGATGAAAAGACACTTAAAAACCAACAAGTTCTTGAAGTAGCTAGAACAGAAGGAGAGGGAGCTAAAGAAGAAAATTGCAGGGAACTGGTAATGGACAGTTCAGGCGGCACCGACAAAGTTGGTTCCCCAAATGATAATTGCTCCTCATCAGCTAAATCCGAGGTTGAACCCAGATGTTGTTTACAAAAGGAAAGTTCTAATGTACATATTGAAGGTGCTCAAATGAATTGCTTGTCATCTGGAAGAAACAATGATACTTCACCACAGAAAGAAAAAGATGAAGTGGGAAGAAAGGCAGGTCATAGTAATAGCTCCCTGCATATAGAAAAAGATGATGCTCACATGAGTAATCAGGCACTCAGTGATGTATCCTTGCTGAGTACACTAACTGCAATAGGTGGTGATGATTCCAAGAGTGAGTGGGAAAATCCAACACAGCAAAGAGCTGATGCCCTGGAGTCTCTGCTTGAGCTATGTGCACGGCTACTAAAGCAGGACAAACTTGAAGAGCTTGCTGGGGTTCTAAGACCATTTGGAGAAGATGCTGTCTCATCTAGAGAAACTGCTATCTGGTTGACAAAGAGTCTCATGTCTGCACAGAAAGATAATGGAGGATCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

766

Amino Acids

84.58

Weight (kDa)

5.84

Isoelectric Point (pI)

46.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	62 - 312	8e-67	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	62 - 309	3e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20860
fragaria_vesca	FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080 FvH4_6g23080
malus_domestica	MD12G1065800.v1.1 MD14G1065800.v1.1
prunus_persica	Prupe.7G068700_v2.0.a1
pyrus_communis	pycom12g05710 pycom14g05320
rosa_chinensis	RchiOBHm_Chr3g0480931
rosa_laevigata	RLG00000023440
rosa_multiflora	Rmu_sc0006028.1_g000015 Rmu_sc0006048.1_g000005
rosa_roxburghii	Rroxscaffold_6G00400870
rosa_rugosa	Rorug03G0187700 Rorug03G0187800 Rorug03G0187900 Rorug03G0188000
rosa_samantha	Rh3AG239200 Rh3BG273400 Rh3CG269000 Rh3DG265000
rosa_wichuraiana	Rw3G021400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1561
Acc36I	ACCTGC	3 cut(s)	775, 1630, 1967
AccB1I	GGYRCC	1 cut(s)	1787
AccB7I	CCANNNNNTGG	2 cut(s)	319, 1265
AccI	GTMKAC	1 cut(s)	1540
AccII	CGCG	1 cut(s)	22
AciI	CCGC	4 cut(s)	47, 216, 961, 1785
AclWI	GGATC	4 cut(s)	1208, 1271, 1366, 2289
AcsI	RAATTY	2 cut(s)	130, 1633
AcuI	CTGAAG	1 cut(s)	1206
AcyI	GRCGYC	1 cut(s)	1530
AdeI	CACNNNGTG	1 cut(s)	2042
AfaI	GTAC	8 cut(s)	155, 1183, 1228, 1423, 1591, 1630, 1884, 2062
AfiI	CCNNNNNNNGG	5 cut(s)	319, 580, 1052, 1265, 1599
AflIII	ACRYGT	1 cut(s)	672
AhdI	GACNNNNNGTC	1 cut(s)	2262
AhlI	ACTAGT	1 cut(s)	1424
AjnI	CCWGG	1 cut(s)	2133
AjuI	GAANNNNNNNTTGG	4 cut(s)	829, 861, 1154, 1186
AleI	CACNNNNGTG	1 cut(s)	733
Alw21I	GWGCWC	4 cut(s)	687, 781, 1900, 2161
Alw26I	GTCTC	7 cut(s)	381, 1062, 1112, 1547, 2145, 2233, 2270
Alw44I	GTGCAC	1 cut(s)	2157
AlwI	GGATC	4 cut(s)	1208, 1271, 1366, 2289
AoxI	GGCC	2 cut(s)	450, 1268
ApaLI	GTGCAC	1 cut(s)	2157
ApeKI	GCWGC	5 cut(s)	505, 755, 791, 911, 1079
ApoI	RAATTY	2 cut(s)	130, 1633
ArsI	GACNNNNNNTTYG	2 cut(s)	106, 138
Asp700I	GAANNNNTTC	2 cut(s)	32, 1104
AspLEI	GCGC	2 cut(s)	24, 796
AspS9I	GGNCC	4 cut(s)	1256, 1268, 1370, 1481
AsuHPI	GGTGA	7 cut(s)	53, 302, 437, 614, 1023, 1932, 2093
AsuNHI	GCTAGC	2 cut(s)	508, 1433
AvaII	GGWCC	3 cut(s)	1256, 1370, 1481
BaeGI	GKGCMC	1 cut(s)	2161
BamHI	GGATCC	1 cut(s)	2294
BanI	GGYRCC	1 cut(s)	1787
BanII	GRGCYC	2 cut(s)	687, 781
Bbv12I	GWGCWC	4 cut(s)	687, 781, 1900, 2161
BbvI	GCAGC	5 cut(s)	492, 742, 778, 898, 1066
BceAI	ACGGC	2 cut(s)	721, 2177
BcgI	CGANNNNNNTGC	2 cut(s)	515, 549
BciT130I	CCWGG	1 cut(s)	2135
BciVI	GTATCC	1 cut(s)	2059
BcoDI	GTCTC	7 cut(s)	381, 1062, 1112, 1547, 2145, 2233, 2270
BcuI	ACTAGT	1 cut(s)	1424
BfmI	CTRYAG	2 cut(s)	912, 1438
BfuAI	ACCTGC	3 cut(s)	775, 1630, 1967
BfuI	GTATCC	1 cut(s)	2059
BglII	AGATCT	1 cut(s)	637
BisI	GCNGC	8 cut(s)	216, 506, 756, 792, 912, 961, 1080, 1786
BlsI	GCNGC	8 cut(s)	217, 507, 757, 793, 913, 962, 1081, 1787
BmcAI	AGTACT	1 cut(s)	1183
Bme1390I	CCNGG	1 cut(s)	2135
Bme18I	GGWCC	3 cut(s)	1256, 1370, 1481
BmeRI	GACNNNNNGTC	1 cut(s)	2262
BmgT120I	GGNCC	4 cut(s)	1256, 1268, 1370, 1481
BmiI	GGNNCC	5 cut(s)	214, 1236, 1789, 1804, 2296
BmrFI	CCNGG	1 cut(s)	2135
BmtI	GCTAGC	2 cut(s)	512, 1437
BpmI	CTGGAG	2 cut(s)	87, 2156
Bpu10I	CCTNAGC	2 cut(s)	681, 881
BpuEI	CTTGAG	1 cut(s)	2168
BsaHI	GRCGYC	1 cut(s)	1530
BsaI	GGTCTC	1 cut(s)	1112
BsaJI	CCNNGG	3 cut(s)	37, 1839, 2133
BsaXI	ACNNNNNCTCC	8 cut(s)	56, 86, 378, 408, 803, 833, 933, 963
Bsc4I	CCNNNNNNNGG	5 cut(s)	319, 580, 1052, 1265, 1599
Bse1I	ACTGG	4 cut(s)	142, 986, 1461, 1770
Bse3DI	GCAATG	4 cut(s)	970, 1155, 1303, 1405
BseBI	CCWGG	1 cut(s)	2135
BseDI	CCNNGG	3 cut(s)	37, 1839, 2133
BseGI	GGATG	5 cut(s)	343, 591, 928, 1108, 1687
BseLI	CCNNNNNNNGG	5 cut(s)	319, 580, 1052, 1265, 1599
BseMI	GCAATG	4 cut(s)	970, 1155, 1303, 1405
BseMII	CTCAG	6 cut(s)	41, 426, 672, 895, 2048, 2053
BseNI	ACTGG	4 cut(s)	142, 986, 1461, 1770
BseRI	GAGGAG	3 cut(s)	488, 819, 1813
BseSI	GKGCMC	1 cut(s)	2161
BseXI	GCAGC	5 cut(s)	492, 742, 778, 898, 1066
BseYI	CCCAGC	1 cut(s)	2195
BsgI	GTGCAG	2 cut(s)	741, 2259
Bsh1236I	CGCG	1 cut(s)	22
BshFI	GGCC	2 cut(s)	452, 1270
BshNI	GGYRCC	1 cut(s)	1787
BsiHKAI	GWGCWC	4 cut(s)	687, 781, 1900, 2161
BslFI	GGGAC	1 cut(s)	252
BslI	CCNNNNNNNGG	5 cut(s)	319, 580, 1052, 1265, 1599
BsmAI	GTCTC	7 cut(s)	381, 1062, 1112, 1547, 2145, 2233, 2270
BsmFI	GGGAC	1 cut(s)	252
BsnI	GGCC	2 cut(s)	452, 1270
Bso31I	GGTCTC	1 cut(s)	1112
Bsp1286I	GDGCHC	4 cut(s)	687, 781, 1900, 2161
Bsp1407I	TGTACA	2 cut(s)	153, 1882
Bsp143I	GATC	7 cut(s)	60, 69, 637, 1213, 1276, 1358, 2294
Bsp19I	CCATGG	1 cut(s)	37
BspACI	CCGC	4 cut(s)	47, 216, 961, 1785
BspANI	GGCC	2 cut(s)	452, 1270
BspCNI	CTCAG	6 cut(s)	42, 427, 673, 894, 2049, 2052
BspFNI	CGCG	1 cut(s)	22
BspLI	GGNNCC	5 cut(s)	214, 1236, 1789, 1804, 2296
BspMAI	CTGCAG	1 cut(s)	916
BspMI	ACCTGC	3 cut(s)	775, 1630, 1967
BspOI	GCTAGC	2 cut(s)	512, 1437
BspPI	GGATC	4 cut(s)	1208, 1271, 1366, 2289
BspQI	GCTCTTC	1 cut(s)	2181
BspT107I	GGYRCC	1 cut(s)	1787
BspTNI	GGTCTC	1 cut(s)	1112
BsrDI	GCAATG	4 cut(s)	970, 1155, 1303, 1405
BsrGI	TGTACA	2 cut(s)	153, 1882
BsrI	ACTGG	4 cut(s)	142, 986, 1461, 1770
BssECI	CCNNGG	3 cut(s)	37, 1839, 2133
BssMI	GATC	7 cut(s)	60, 69, 637, 1213, 1276, 1358, 2294
BssNAI	GTATAC	1 cut(s)	1541
BssNI	GRCGYC	1 cut(s)	1530
BssT1I	CCWWGG	1 cut(s)	37
Bst1107I	GTATAC	1 cut(s)	1541
Bst2UI	CCWGG	1 cut(s)	2135
Bst4CI	ACNGT	4 cut(s)	392, 815, 1231, 1778
Bst6I	CTCTTC	4 cut(s)	837, 881, 1133, 2181
BstACI	GRCGYC	1 cut(s)	1530
BstAUI	TGTACA	2 cut(s)	153, 1882
BstC8I	GCNNGC	9 cut(s)	94, 510, 878, 1043, 1413, 1435, 1524, 1660, 2193
BstDEI	CTNAG	8 cut(s)	50, 279, 435, 681, 881, 2039, 2057, 2204
BstDSI	CCRYGG	1 cut(s)	37
BstEII	GGTNACC	1 cut(s)	41
BstENI	CCTNNNNNAGG	1 cut(s)	578
BstF5I	GGATG	5 cut(s)	343, 591, 928, 1108, 1687
BstFNI	CGCG	1 cut(s)	22
BstHHI	GCGC	2 cut(s)	24, 796
BstKTI	GATC	7 cut(s)	63, 72, 640, 1216, 1279, 1361, 2297
BstMAI	GTCTC	7 cut(s)	381, 1062, 1112, 1547, 2145, 2233, 2270
BstMBI	GATC	7 cut(s)	60, 69, 637, 1213, 1276, 1358, 2294
BstMWI	GCNNNNNNNGC	4 cut(s)	212, 776, 1062, 2170
BstNI	CCWGG	1 cut(s)	2135
BstNSI	RCATGY	2 cut(s)	676, 880
BstPI	GGTNACC	1 cut(s)	41
BstSCI	CCNGG	1 cut(s)	2133
BstSFI	CTRYAG	2 cut(s)	912, 1438
BstSLI	GKGCMC	1 cut(s)	2161
BstUI	CGCG	1 cut(s)	22
BstV1I	GCAGC	5 cut(s)	492, 742, 778, 898, 1066
BstX2I	RGATCY	2 cut(s)	637, 2294
BstXI	CCANNNNNNTGG	1 cut(s)	1096
BstYI	RGATCY	2 cut(s)	637, 2294
BstZ17I	GTATAC	1 cut(s)	1541
BsuI	GTATCC	1 cut(s)	2059
BsuRI	GGCC	2 cut(s)	452, 1270
BtgI	CCRYGG	1 cut(s)	37
BtsCI	GGATG	5 cut(s)	343, 591, 928, 1108, 1687
BtsI	GCAGTG	1 cut(s)	1248
BtsIMutI	CAGTG	5 cut(s)	854, 1248, 1350, 1468, 2047
BveI	ACCTGC	3 cut(s)	775, 1630, 1967
Cac8I	GCNNGC	9 cut(s)	94, 510, 878, 1043, 1413, 1435, 1524, 1660, 2193
CfoI	GCGC	2 cut(s)	24, 796
Cfr13I	GGNCC	4 cut(s)	1256, 1268, 1370, 1481
CseI	GACGC	2 cut(s)	1275, 1538
Csp6I	GTAC	8 cut(s)	154, 1182, 1227, 1422, 1590, 1629, 1883, 2061
CviAII	CATG	9 cut(s)	38, 77, 430, 673, 787, 877, 1561, 2023, 2269
CviQI	GTAC	8 cut(s)	154, 1182, 1227, 1422, 1590, 1629, 1883, 2061
DdeI	CTNAG	8 cut(s)	50, 279, 435, 681, 881, 2039, 2057, 2204
DpnI	GATC	7 cut(s)	62, 71, 639, 1215, 1278, 1360, 2296
DpnII	GATC	7 cut(s)	60, 69, 637, 1213, 1276, 1358, 2294
DraI	TTTAAA	1 cut(s)	999
DraIII	CACNNNGTG	1 cut(s)	2042
DrdI	GACNNNNNNGTC	1 cut(s)	1561
DriI	GACNNNNNGTC	1 cut(s)	2262
DseDI	GACNNNNNNGTC	1 cut(s)	1561
Eam1104I	CTCTTC	4 cut(s)	837, 881, 1133, 2181
Eam1105I	GACNNNNNGTC	1 cut(s)	2262
EarI	CTCTTC	4 cut(s)	837, 881, 1133, 2181
Ecl136II	GAGCTC	2 cut(s)	685, 779
Eco130I	CCWWGG	1 cut(s)	37
Eco24I	GRGCYC	2 cut(s)	687, 781
Eco31I	GGTCTC	1 cut(s)	1112
Eco47I	GGWCC	3 cut(s)	1256, 1370, 1481
Eco53kI	GAGCTC	2 cut(s)	685, 779
Eco57I	CTGAAG	1 cut(s)	1206
Eco91I	GGTNACC	1 cut(s)	41
EcoICRI	GAGCTC	2 cut(s)	685, 779
EcoNI	CCTNNNNNAGG	1 cut(s)	578
EcoO65I	GGTNACC	1 cut(s)	41
EcoRII	CCWGG	1 cut(s)	2133
EcoT14I	CCWWGG	1 cut(s)	37
EcoT22I	ATGCAT	2 cut(s)	401, 970
EcoT38I	GRGCYC	2 cut(s)	687, 781
ErhI	CCWWGG	1 cut(s)	37
FaeI	CATG	9 cut(s)	41, 80, 433, 676, 790, 880, 1564, 2026, 2272
FalI	AAGNNNNNCTT	2 cut(s)	1179, 1211
FaqI	GGGAC	1 cut(s)	252
FatI	CATG	9 cut(s)	37, 76, 429, 672, 786, 876, 1560, 2022, 2268
FauI	CCCGC	1 cut(s)	54
FblI	GTMKAC	1 cut(s)	1540
Fnu4HI	GCNGC	8 cut(s)	216, 506, 756, 792, 912, 961, 1080, 1786
FokI	GGATG	5 cut(s)	330, 578, 915, 1095, 1694
FriOI	GRGCYC	2 cut(s)	687, 781
Fsp4HI	GCNGC	8 cut(s)	216, 506, 756, 792, 912, 961, 1080, 1786
GlaI	GCGC	2 cut(s)	23, 795
GluI	GCNGC	8 cut(s)	216, 506, 756, 792, 912, 961, 1080, 1786
GsaI	CCCAGC	1 cut(s)	2199
GsuI	CTGGAG	2 cut(s)	87, 2156
HaeIII	GGCC	2 cut(s)	452, 1270
HgaI	GACGC	2 cut(s)	1275, 1538
HhaI	GCGC	2 cut(s)	24, 796
Hin1I	GRCGYC	1 cut(s)	1530
Hin1II	CATG	9 cut(s)	41, 80, 433, 676, 790, 880, 1564, 2026, 2272
Hin6I	GCGC	2 cut(s)	22, 794
HinP1I	GCGC	2 cut(s)	22, 794
HincII	GTYRAC	3 cut(s)	495, 517, 2256
HindII	GTYRAC	3 cut(s)	495, 517, 2256
HindIII	AAGCTT	1 cut(s)	1193
HphI	GGTGA	7 cut(s)	53, 302, 437, 614, 1023, 1932, 2093
Hpy166II	GTNNAC	8 cut(s)	379, 495, 517, 1541, 1862, 2063, 2159, 2256
Hpy8I	GTNNAC	8 cut(s)	379, 495, 517, 1541, 1862, 2063, 2159, 2256
HpyAV	CCTTC	6 cut(s)	48, 413, 442, 962, 1724, 1886
HpyCH4III	ACNGT	4 cut(s)	392, 815, 1231, 1778
HpyF10VI	GCNNNNNNNGC	4 cut(s)	212, 776, 1062, 2170
HpyF3I	CTNAG	8 cut(s)	50, 279, 435, 681, 881, 2039, 2057, 2204
Hsp92I	GRCGYC	1 cut(s)	1530
Hsp92II	CATG	9 cut(s)	41, 80, 433, 676, 790, 880, 1564, 2026, 2272
HspAI	GCGC	2 cut(s)	22, 794
Kzo9I	GATC	7 cut(s)	60, 69, 637, 1213, 1276, 1358, 2294
LguI	GCTCTTC	1 cut(s)	2181
LmnI	GCTCC	7 cut(s)	148, 212, 690, 784, 1739, 1826, 1997
Lsp1109I	GCAGC	5 cut(s)	492, 742, 778, 898, 1066
MaeIII	GTNAC	4 cut(s)	41, 403, 425, 726
MalI	GATC	7 cut(s)	62, 71, 639, 1215, 1278, 1360, 2296
MboI	GATC	7 cut(s)	60, 69, 637, 1213, 1276, 1358, 2294
MfeI	CAATTG	4 cut(s)	500, 837, 1272, 1643
MflI	RGATCY	2 cut(s)	637, 2294
MhlI	GDGCHC	4 cut(s)	687, 781, 1900, 2161
MlyI	GAGTC	6 cut(s)	109, 490, 898, 1660, 2147, 2272
MmeI	TCCRAC	5 cut(s)	126, 619, 637, 1508, 2135
Mph1103I	ATGCAT	2 cut(s)	401, 970
MroXI	GAANNNNTTC	2 cut(s)	32, 1104
MseI	TTAA	8 cut(s)	260, 326, 338, 555, 810, 923, 998, 1695
MslI	CAYNNNNRTG	2 cut(s)	549, 733
MspA1I	CMGCKG	2 cut(s)	49, 911
MspR9I	CCNGG	1 cut(s)	2135
MunI	CAATTG	4 cut(s)	500, 837, 1272, 1643
MvaI	CCWGG	1 cut(s)	2135
MvnI	CGCG	1 cut(s)	22
MwoI	GCNNNNNNNGC	4 cut(s)	212, 776, 1062, 2170
NcoI	CCATGG	1 cut(s)	37
NdeII	GATC	7 cut(s)	60, 69, 637, 1213, 1276, 1358, 2294
NheI	GCTAGC	2 cut(s)	508, 1433
NlaIII	CATG	9 cut(s)	41, 80, 433, 676, 790, 880, 1564, 2026, 2272
NlaIV	GGNNCC	5 cut(s)	214, 1236, 1789, 1804, 2296
NmeAIII	GCCGAG	1 cut(s)	1091
NmuCI	GTSAC	3 cut(s)	41, 425, 726
NsiI	ATGCAT	2 cut(s)	401, 970
NspI	RCATGY	2 cut(s)	676, 880
OliI	CACNNNNGTG	1 cut(s)	733
PaeI	GCATGC	1 cut(s)	880
PciI	ACATGT	1 cut(s)	672
PciSI	GCTCTTC	1 cut(s)	2181
PdmI	GAANNNNTTC	2 cut(s)	32, 1104
PfeI	GAWTC	4 cut(s)	268, 541, 1490, 2087
PflMI	CCANNNNNTGG	2 cut(s)	319, 1265
PkrI	GCNGC	8 cut(s)	217, 507, 757, 793, 913, 962, 1081, 1787
PleI	GAGTC	6 cut(s)	109, 490, 897, 1660, 2146, 2271
PpsI	GAGTC	6 cut(s)	109, 490, 897, 1660, 2146, 2271
PscI	ACATGT	1 cut(s)	672
Psp124BI	GAGCTC	2 cut(s)	687, 781
Psp6I	CCWGG	1 cut(s)	2133
PspEI	GGTNACC	1 cut(s)	41
PspFI	CCCAGC	1 cut(s)	2195
PspGI	CCWGG	1 cut(s)	2133
PspN4I	GGNNCC	5 cut(s)	214, 1236, 1789, 1804, 2296
PspPI	GGNCC	4 cut(s)	1256, 1268, 1370, 1481
PstI	CTGCAG	1 cut(s)	916
PsuI	RGATCY	2 cut(s)	637, 2294
PvuII	CAGCTG	1 cut(s)	911
RsaI	GTAC	8 cut(s)	155, 1183, 1228, 1423, 1591, 1630, 1884, 2062
RsaNI	GTAC	8 cut(s)	154, 1182, 1227, 1422, 1590, 1629, 1883, 2061
RseI	CAYNNNNRTG	2 cut(s)	549, 733
SacI	GAGCTC	2 cut(s)	687, 781
SapI	GCTCTTC	1 cut(s)	2181
SaqAI	TTAA	8 cut(s)	260, 326, 338, 555, 810, 923, 998, 1695
SatI	GCNGC	8 cut(s)	216, 506, 756, 792, 912, 961, 1080, 1786
Sau3AI	GATC	7 cut(s)	60, 69, 637, 1213, 1276, 1358, 2294
Sau96I	GGNCC	4 cut(s)	1256, 1268, 1370, 1481
ScaI	AGTACT	1 cut(s)	1183
SchI	GAGTC	6 cut(s)	109, 490, 898, 1660, 2147, 2272
ScrFI	CCNGG	1 cut(s)	2135
SduI	GDGCHC	4 cut(s)	687, 781, 1900, 2161
SfcI	CTRYAG	2 cut(s)	912, 1438
SinI	GGWCC	3 cut(s)	1256, 1370, 1481
SmiMI	CAYNNNNRTG	2 cut(s)	549, 733
SmlI	CTYRAG	1 cut(s)	2147
SmoI	CTYRAG	1 cut(s)	2147
SpeI	ACTAGT	1 cut(s)	1424
SphI	GCATGC	1 cut(s)	880
SsiI	CCGC	4 cut(s)	47, 216, 961, 1785
SstI	GAGCTC	2 cut(s)	687, 781
StyD4I	CCNGG	1 cut(s)	2133
StyI	CCWWGG	1 cut(s)	37
TaaI	ACNGT	4 cut(s)	392, 815, 1231, 1778
TaqI	TCGA	2 cut(s)	63, 1116
TatI	WGTACW	7 cut(s)	153, 1181, 1226, 1421, 1628, 1882, 2060
TauI	GCSGC	3 cut(s)	218, 963, 1788
TfiI	GAWTC	4 cut(s)	268, 541, 1490, 2087
Tru1I	TTAA	8 cut(s)	260, 326, 338, 555, 810, 923, 998, 1695
Tru9I	TTAA	8 cut(s)	260, 326, 338, 555, 810, 923, 998, 1695
TscAI	CASTG	5 cut(s)	861, 1255, 1357, 1468, 2047
TseFI	GTSAC	3 cut(s)	41, 425, 726
TseI	GCWGC	5 cut(s)	505, 755, 791, 911, 1079
Tsp45I	GTSAC	3 cut(s)	41, 425, 726
TspGWI	ACGGA	1 cut(s)	302
TspRI	CASTG	5 cut(s)	861, 1255, 1357, 1468, 2047
Van91I	CCANNNNNTGG	2 cut(s)	319, 1265
VneI	GTGCAC	1 cut(s)	2157
VpaK11BI	GGWCC	3 cut(s)	1256, 1370, 1481
XagI	CCTNNNNNAGG	1 cut(s)	578
XapI	RAATTY	2 cut(s)	130, 1633
XbaI	TCTAGA	1 cut(s)	2234
XceI	RCATGY	2 cut(s)	676, 880
XmiI	GTMKAC	1 cut(s)	1540
XmnI	GAANNNNTTC	2 cut(s)	32, 1104
ZrmI	AGTACT	1 cut(s)	1183
Zsp2I	ATGCAT	2 cut(s)	401, 970

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.