Rorug03G0188000

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003863
Physical Location & Seq
Reverse (-)
16359257 .. 16364062
4806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0188000.1

Sequence Viewer

Length: 2697 bp
ATGGGTTCCTTTGCGGGTACTTCTGAGATTGTTGAAGCGACAGACGAGCCCAGTTCAAGTCAATATTCTCGTGGAACATATCGACCAAATACAGGTTTGGGTGAGAATGATAGGAAGCTTCCTGCACTTAAACTAGGATCATCCAGGGATTCTATAGAAGATGATATTAATAAGCTTTTTGCGGCAATCCATCTCAAAACTTCATCCAAGGGTTCAGATCATTCGAAGCAAGTAGGCACTAGCCCTTTGAATAAAAATGCCTTAAAGAAGCCAATTACAATGGGTGTGTCTCACTCACCAAGGTTTGGGACTTCTGAGCCAGCAACTCTAAAGCAGGCATTAAGGGAGCTGTCTATTACCAAGGCATCGGAAATGGCTGCTATGAAACGGTTAACAAAGTTGACAAGTCCTTCAAGAGCCTCTGAAGCTGGGAGGATCAAGACATTGTACAATGCGGTTGTTGTTCAAGCCACTCAATGTGGTCCTTCCTCACATGAAGACAGGGGAAATATAGTTGAAATATCTCTGGTCCCAGAAGAAAGCACATCATATTCTCGTCAGAAGATGCCTGATCATCCTCCAATGCCCAAGACGAAGTCATCAAGCCAGAGTGCACATTCTTCTCCTCGATTTGTAAATGGGACAACGGGAACTAGTGCTGGGAGCACAGCAGTACAAAATGAAAGTGCTTCCACGTTGAGGACAGTTGGGGTACAAACAATAAAGTCGGAGCTGGGTCTGGAAGATAAAAATGAATCTGGTAGCGACCTTATTTCAGAAGTTGCTGAAAATTTACCTGCCAAAGCTGTTTCGAAGACAACATTAAAGGCAGAGCTGGGGAAGAAAGAGAAACATAAATCTACAAAGGAAAATAATTCAGTATCTGCTCGAAATAGTCCTACCAAAACCAAGTTAGCAAATAAGGTCTCAGCAGCAAAACCGGGGCGAAAAGTCAAGGTGCATGGAGTGCCTTCTTCATCTAGCTTCGTCAATGGAAATGTGGCAAGCAAGTTTTCCAGAAACACCCTCCGCAGTGTCAAACCATCCAGCAGGAATAAAAGTATTCTAAAGAAGAAGCAAAAACAAGATTCAATGTCTCCTGCCTGCAAATCTAGTAAGAATAATGAAGCTGATGCTGATATTGTTGCATGTACAAGTCAGCTGGTCTGTGAAAGATGCCATATTGCCCTAAGAGGTGCCGGAAAACAATCCAATCAAGCTTTGGCAACCCCCAGTCTTGCTACTGATATAAGCTCAAGCAATGTACGGTCCAGTCCAAGTAAACCAGGCTTCACCGTAGATACAGGTGGGGGTAATGTCGCAAAAGCAAAAAAGAACCCAAAATCAAAAGGAACAGGGGAGTTCTCCCAAAGCTCAAAAAGTAGCCAAGGTGAGTATAGTAGTAGTACGAGTTTGAGTGATGAGAGTAATTTAAGTAGGACTAGTTGTAGCAATAAACCTCACATGTCGAAGGATGTGAGGTGGGAAGCCATCCGTCATGTTCGGACGCAGTATGGAGCCCTGGGCTTGAGACACTTTAATCTTTTGAAGAAGCTTGGTTGTGGAGACATTGGAACTGTATATCTTGCTGAGCTAATCAGTACAAACTGCCTTTTCGCCATAAAGGTAATGGACAATGAGTTCCTGGCAAGAAGGAAGAAGATGCCAAGGGCTCACACAGAAAGTGAAATACTGAGGATGCTTGATCATCCTTTTCTCCCTACATTGTATTCCCAGTTTACATCAGATAATCTTTCATGTTTAGTAATGGAGTATTGTCCTGGTGGAGATCTTCATGTCCTCCGGCAGAAACAGGTCGGAAGGTGTTTTTCTGAAGCAGCTACAAGGTTTTATGTGGCTGAAGTCCTGCTTGCTTTGGAGTACTTGCACATGCTTGGTGTAGTTTATCGGGATCTGAAACCGGAAAACATTCTTGTCCGGGAAGATGGTCACATAATGCTTACAGATTTTGACCTGTCTCTCAGATGCTCTGTCAGTCCAACTCTTTTGAAATCACCTTCATGTGATGCAGATCCTGCGAGAATGTCTGGTCCGTGTACAGCATCTAACTGCGCTGAGCCCTTCTGCATTGAACCCTCCTCTTGTCGAGTCTCATGCTTCAGCCCTAGGTTTCTACCTGCTGCCGCAAAAACAAGGAAGTTGAAAAATGACCCTACATCTCAAGTCAGATCATTGCCACAGCTTGTGGCGGAGCCTACCGACGCACGGTCCAATTCCTTTGTTGGGACCCACGAGTACTTGGCCCCCGAGATCATCAAAGGAGACGGTCATGGTGCTGCTGTTGATTGGTGGACGTTTGGAATCTTTCTTTACGAGCTTCTATATGGCAGAACACCGTTTAAGGGTGTTAATAACGAGGACACATTAGGCAATGTGGTCATGCAGAGCCTAAGGTTCCCCGACACCCCGATTGTGAGCTTCCAGGCTAGGGATCTCATCAGAGGGCTGTTGGTAAAGGAACCAGAGAATCGGTTGGGCACGGAGAAAGGAGCTGGTGAGATTAAGCAGCATCCGTTCTTTGAAGGTTTGAATTGGGCGCTAATCCGCTGTGCTGTTCCACCCGAACTGCCTGATTTTTGCGACTTTGAAGTTCCAGACATGCCTGCTCAGGACGATAACGCCAAGTACTTAGAGTTCAAAGCTACCGGAGAGCACCTAGAGTTTGAGTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

898

Amino Acids

97.74

Weight (kDa)

9.11

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 513 - 664 4.4e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 514 - 663 2.3e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 744 - 847 3.7e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 751 - 832 4.5e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 805, 2146
AccB1I GGYRCC 1 cut(s) 1196
AccB7I CCANNNNNTGG 1 cut(s) 305
AciI CCGC 7 cut(s) 14, 182, 455, 1030, 2145, 2210, 2566
AclWI GGATC 5 cut(s) 145, 443, 1920, 2027, 2460
AcsI RAATTY 1 cut(s) 790
AcuI CTGAAG 4 cut(s) 444, 1854, 1881, 2104
AfiI CCNNNNNNNGG 7 cut(s) 92, 305, 699, 2226, 2244, 2363, 2449
AflIII ACRYGT 1 cut(s) 1464
AhdI GACNNNNNGTC 1 cut(s) 2227
AhlI ACTAGT 2 cut(s) 653, 1442
AjnI CCWGG 6 cut(s) 143, 1285, 1521, 1644, 1780, 2442
AloI GAACNNNNNNTCC 2 cut(s) 1625, 1657
Alw21I GWGCWC 3 cut(s) 616, 668, 2676
Alw26I GTCTC 8 cut(s) 294, 931, 1101, 1525, 1560, 1983, 2116, 2277
Alw44I GTGCAC 1 cut(s) 612
AlwI GGATC 5 cut(s) 145, 443, 1920, 2027, 2460
AlwNI CAGNNNCTG 2 cut(s) 884, 2036
Ama87I CYCGRG 1 cut(s) 2267
AoxI GGCC 1 cut(s) 2262
ApaLI GTGCAC 1 cut(s) 612
ApeKI GCWGC 6 cut(s) 377, 932, 1838, 2141, 2297, 2527
ApoI RAATTY 1 cut(s) 790
AseI ATTAAT 1 cut(s) 168
Asp700I GAANNNNTTC 1 cut(s) 1929
AspA2I CCTAGG 1 cut(s) 2126
AspLEI GCGC 2 cut(s) 2075, 2560
AspS9I GGNCC 7 cut(s) 482, 529, 1269, 2051, 2229, 2247, 2263
AsuC2I CCSGG 2 cut(s) 942, 1940
AsuHPI GGTGA 6 cut(s) 113, 288, 1285, 1403, 2007, 2528
AsuII TTCGAA 2 cut(s) 224, 812
AvaI CYCGRG 1 cut(s) 2267
AvaII GGWCC 6 cut(s) 482, 529, 1269, 2051, 2229, 2247
AvrII CCTAGG 1 cut(s) 2126
AxyI CCTNAGG 1 cut(s) 2411
BaeGI GKGCMC 2 cut(s) 616, 2501
BanI GGYRCC 1 cut(s) 1196
BanII GRGCYC 4 cut(s) 51, 1522, 1675, 2082
BauI CACGAG 2 cut(s) 69, 2252
BbsI GAAGAC 2 cut(s) 504, 821
Bbv12I GWGCWC 3 cut(s) 616, 668, 2676
BbvI GCAGC 6 cut(s) 364, 944, 1850, 2128, 2284, 2539
BccI CCATC 4 cut(s) 198, 1051, 1499, 1940
BcgI CGANNNNNNTGC 2 cut(s) 2097, 2131
BciT130I CCWGG 6 cut(s) 145, 1287, 1523, 1646, 1782, 2444
BclI TGATCA 2 cut(s) 571, 1705
BcnI CCSGG 2 cut(s) 942, 1940
BcoDI GTCTC 8 cut(s) 294, 931, 1101, 1525, 1560, 1983, 2116, 2277
BcuI ACTAGT 2 cut(s) 653, 1442
BfaI CTAG 9 cut(s) 134, 240, 654, 981, 1113, 1443, 2127, 2448, 2678
BfmI CTRYAG 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 2561
BfuAI ACCTGC 2 cut(s) 805, 2146
BglII AGATCT 1 cut(s) 1789
BisI GCNGC 8 cut(s) 183, 378, 933, 1839, 2142, 2145, 2298, 2528
BlnI CCTAGG 1 cut(s) 2126
BlpI GCTNAGC 2 cut(s) 1590, 2076
BlsI GCNGC 8 cut(s) 184, 379, 934, 1840, 2143, 2146, 2299, 2529
BmcAI AGTACT 3 cut(s) 1883, 2258, 2648
Bme1390I CCNGG 8 cut(s) 145, 942, 1287, 1523, 1646, 1782, 1940, 2444
Bme18I GGWCC 6 cut(s) 482, 529, 1269, 2051, 2229, 2247
BmeRI GACNNNNNGTC 1 cut(s) 2227
BmeT110I CYCGRG 1 cut(s) 2267
BmgT120I GGNCC 7 cut(s) 482, 529, 1269, 2051, 2229, 2247, 2263
BmrFI CCNGG 8 cut(s) 145, 942, 1287, 1523, 1646, 1782, 1940, 2444
BmrI ACTGGG 3 cut(s) 45, 1227, 1729
BmuI ACTGGG 3 cut(s) 45, 1227, 1729
BpiI GAAGAC 2 cut(s) 504, 821
Bpu10I CCTNAGC 1 cut(s) 2628
Bpu1102I GCTNAGC 2 cut(s) 1590, 2076
Bpu14I TTCGAA 2 cut(s) 224, 812
BpuEI CTTGAG 3 cut(s) 1240, 1549, 2166
BpuMI CCSGG 2 cut(s) 942, 1940
BsaBI GATNNNNATC 1 cut(s) 2031
BsaI GGTCTC 1 cut(s) 931
BsaWI WCCGGW 2 cut(s) 1921, 2666
Bsc4I CCNNNNNNNGG 7 cut(s) 92, 305, 699, 2226, 2244, 2363, 2449
Bse1I ACTGG 4 cut(s) 51, 1233, 1272, 1735
Bse21I CCTNAGG 1 cut(s) 2411
Bse3DI GCAATG 3 cut(s) 1267, 2192, 2398
Bse8I GATNNNNATC 1 cut(s) 2031
BseBI CCWGG 6 cut(s) 145, 1287, 1523, 1646, 1782, 2444
BseGI GGATG 9 cut(s) 140, 203, 574, 1043, 1480, 1491, 1704, 1708, 2530
BseJI GATNNNNATC 1 cut(s) 2031
BseLI CCNNNNNNNGG 7 cut(s) 92, 305, 699, 2226, 2244, 2363, 2449
BseMI GCAATG 3 cut(s) 1267, 2192, 2398
BseMII CTCAG 8 cut(s) 15, 306, 942, 1581, 1685, 1996, 2067, 2642
BseNI ACTGG 4 cut(s) 51, 1233, 1272, 1735
BseRI GAGGAG 2 cut(s) 615, 2089
BseSI GKGCMC 2 cut(s) 616, 2501
BseXI GCAGC 6 cut(s) 364, 944, 1850, 2128, 2284, 2539
BseYI CCCAGC 4 cut(s) 428, 659, 733, 835
BsgI GTGCAG 1 cut(s) 108
BshFI GGCC 1 cut(s) 2264
BshNI GGYRCC 1 cut(s) 1196
BsiHKAI GWGCWC 3 cut(s) 616, 668, 2676
BsiHKCI CYCGRG 1 cut(s) 2267
BsiSI CCGG 6 cut(s) 941, 1200, 1804, 1922, 1939, 2667
BslFI GGGAC 4 cut(s) 322, 515, 655, 2260
BslI CCNNNNNNNGG 7 cut(s) 92, 305, 699, 2226, 2244, 2363, 2449
BsmAI GTCTC 8 cut(s) 294, 931, 1101, 1525, 1560, 1983, 2116, 2277
BsmBI CGTCTC 1 cut(s) 2277
BsmFI GGGAC 4 cut(s) 322, 515, 655, 2260
BsnI GGCC 1 cut(s) 2264
Bso31I GGTCTC 1 cut(s) 931
BsoBI CYCGRG 1 cut(s) 2267
Bsp119I TTCGAA 2 cut(s) 224, 812
Bsp1286I GDGCHC 8 cut(s) 51, 616, 668, 1522, 1675, 2082, 2501, 2676
Bsp1407I TGTACA 3 cut(s) 447, 1151, 2057
Bsp1720I GCTNAGC 2 cut(s) 1590, 2076
BspACI CCGC 7 cut(s) 14, 182, 455, 1030, 2145, 2210, 2566
BspANI GGCC 1 cut(s) 2264
BspCNI CTCAG 8 cut(s) 16, 307, 941, 1582, 1686, 1995, 2068, 2641
BspMI ACCTGC 2 cut(s) 805, 2146
BspPI GGATC 5 cut(s) 145, 443, 1920, 2027, 2460
BspT104I TTCGAA 2 cut(s) 224, 812
BspT107I GGYRCC 1 cut(s) 1196
BspTNI GGTCTC 1 cut(s) 931
BsrDI GCAATG 3 cut(s) 1267, 2192, 2398
BsrGI TGTACA 3 cut(s) 447, 1151, 2057
BsrI ACTGG 4 cut(s) 51, 1233, 1272, 1735
BssSI CACGAG 2 cut(s) 69, 2252
BssT1I CCWWGG 6 cut(s) 207, 299, 360, 1387, 1667, 2126
Bst2BI CACGAG 2 cut(s) 69, 2252
Bst2UI CCWGG 6 cut(s) 145, 1287, 1523, 1646, 1782, 2444
Bst4CI ACNGT 8 cut(s) 390, 706, 1269, 1297, 1579, 2229, 2288, 2358
BstAPI GCANNNNNTGC 2 cut(s) 967, 2036
BstAUI TGTACA 3 cut(s) 447, 1151, 2057
BstBI TTCGAA 2 cut(s) 224, 812
BstC8I GCNNGC 6 cut(s) 321, 336, 1006, 1105, 1872, 2625
BstF5I GGATG 9 cut(s) 140, 203, 574, 1043, 1480, 1491, 1704, 1708, 2530
BstH2I RGCGCY 1 cut(s) 2561
BstHHI GCGC 2 cut(s) 2075, 2560
BstMAI GTCTC 8 cut(s) 294, 931, 1101, 1525, 1560, 1983, 2116, 2277
BstMWI GCNNNNNNNGC 3 cut(s) 425, 967, 2036
BstNI CCWGG 6 cut(s) 145, 1287, 1523, 1646, 1782, 2444
BstNSI RCATGY 4 cut(s) 1152, 1468, 1894, 2623
BstSCI CCNGG 8 cut(s) 143, 940, 1285, 1521, 1644, 1780, 1938, 2442
BstSFI CTRYAG 1 cut(s) 153
BstSLI GKGCMC 2 cut(s) 616, 2501
BstV1I GCAGC 6 cut(s) 364, 944, 1850, 2128, 2284, 2539
BstV2I GAAGAC 2 cut(s) 504, 821
BstX2I RGATCY 4 cut(s) 1789, 1912, 2032, 2452
BstYI RGATCY 4 cut(s) 1789, 1912, 2032, 2452
Bsu36I CCTNAGG 1 cut(s) 2411
BsuRI GGCC 1 cut(s) 2264
BtsCI GGATG 9 cut(s) 140, 203, 574, 1043, 1480, 1491, 1704, 1708, 2530
BtsI GCAGTG 1 cut(s) 1039
BtsIMutI CAGTG 1 cut(s) 1039
BveI ACCTGC 2 cut(s) 805, 2146
Cac8I GCNNGC 6 cut(s) 321, 336, 1006, 1105, 1872, 2625
CaiI CAGNNNCTG 2 cut(s) 884, 2036
CfoI GCGC 2 cut(s) 2075, 2560
Cfr13I GGNCC 7 cut(s) 482, 529, 1269, 2051, 2229, 2247, 2263
CseI GACGC 2 cut(s) 1516, 2231
DriI GACNNNNNGTC 1 cut(s) 2227
Eam1105I GACNNNNNGTC 1 cut(s) 2227
EciI GGCGGA 1 cut(s) 2225
Eco130I CCWWGG 6 cut(s) 207, 299, 360, 1387, 1667, 2126
Eco24I GRGCYC 4 cut(s) 51, 1522, 1675, 2082
Eco31I GGTCTC 1 cut(s) 931
Eco47I GGWCC 6 cut(s) 482, 529, 1269, 2051, 2229, 2247
Eco57I CTGAAG 4 cut(s) 444, 1854, 1881, 2104
Eco81I CCTNAGG 1 cut(s) 2411
Eco88I CYCGRG 1 cut(s) 2267
EcoO109I RGGNCCY 1 cut(s) 2247
EcoRII CCWGG 6 cut(s) 143, 1285, 1521, 1644, 1780, 2442
EcoT14I CCWWGG 6 cut(s) 207, 299, 360, 1387, 1667, 2126
EcoT38I GRGCYC 4 cut(s) 51, 1522, 1675, 2082
ErhI CCWWGG 6 cut(s) 207, 299, 360, 1387, 1667, 2126
Esp3I CGTCTC 1 cut(s) 2277
FalI AAGNNNNNCTT 2 cut(s) 1854, 1886
FaqI GGGAC 4 cut(s) 322, 515, 655, 2260
FauI CCCGC 1 cut(s) 7
FbaI TGATCA 2 cut(s) 571, 1705
Fnu4HI GCNGC 8 cut(s) 183, 378, 933, 1839, 2142, 2145, 2298, 2528
FokI GGATG 9 cut(s) 127, 190, 561, 1030, 1478, 1487, 1695, 1711, 2517
FriOI GRGCYC 4 cut(s) 51, 1522, 1675, 2082
Fsp4HI GCNGC 8 cut(s) 183, 378, 933, 1839, 2142, 2145, 2298, 2528
FspBI CTAG 9 cut(s) 134, 240, 654, 981, 1113, 1443, 2127, 2448, 2678
GlaI GCGC 2 cut(s) 2074, 2559
GluI GCNGC 8 cut(s) 183, 378, 933, 1839, 2142, 2145, 2298, 2528
GsaI CCCAGC 4 cut(s) 432, 663, 737, 839
HaeII RGCGCY 1 cut(s) 2561
HaeIII GGCC 1 cut(s) 2264
HapII CCGG 6 cut(s) 941, 1200, 1804, 1922, 1939, 2667
HgaI GACGC 2 cut(s) 1516, 2231
HhaI GCGC 2 cut(s) 2075, 2560
Hin6I GCGC 2 cut(s) 2073, 2558
HinP1I GCGC 2 cut(s) 2073, 2558
HincII GTYRAC 2 cut(s) 393, 402
HindII GTYRAC 2 cut(s) 393, 402
HindIII AAGCTT 4 cut(s) 116, 173, 1218, 1553
HinfI GANTC 6 cut(s) 149, 755, 1088, 2109, 2322, 2488
HpaI GTTAAC 1 cut(s) 393
HpaII CCGG 6 cut(s) 941, 1200, 1804, 1922, 1939, 2667
HphI GGTGA 6 cut(s) 113, 288, 1285, 1403, 2007, 2528
Hpy166II GTNNAC 7 cut(s) 393, 402, 614, 1283, 1740, 2058, 2313
Hpy188III TCNNGA 7 cut(s) 414, 439, 740, 1017, 1910, 2615, 2630
Hpy8I GTNNAC 7 cut(s) 393, 402, 614, 1283, 1740, 2058, 2313
Hpy99I CGWCG 1 cut(s) 2225
HpyAV CCTTC 9 cut(s) 420, 495, 981, 1465, 1647, 1815, 2028, 2092, 2537
HpyCH4III ACNGT 8 cut(s) 390, 706, 1269, 1297, 1579, 2229, 2288, 2358
HpyCH4IV ACGT 2 cut(s) 695, 2315
HpyCH4V TGCA 9 cut(s) 125, 614, 961, 1107, 1148, 1888, 2030, 2088, 2404
HpyF10VI GCNNNNNNNGC 3 cut(s) 425, 967, 2036
HpySE526I ACGT 2 cut(s) 695, 2315
HspAI GCGC 2 cut(s) 2073, 2558
KflI GGGWCCC 1 cut(s) 2247
Ksp22I TGATCA 2 cut(s) 571, 1705
KspAI GTTAAC 1 cut(s) 393
LmnI GCTCC 6 cut(s) 346, 663, 730, 1517, 2212, 2510
Lsp1109I GCAGC 6 cut(s) 364, 944, 1850, 2128, 2284, 2539
MaeI CTAG 9 cut(s) 134, 240, 654, 981, 1113, 1443, 2127, 2448, 2678
MaeII ACGT 2 cut(s) 695, 2315
MaeIII GTNAC 1 cut(s) 1949
MflI RGATCY 4 cut(s) 1789, 1912, 2032, 2452
MhlI GDGCHC 8 cut(s) 51, 616, 668, 1522, 1675, 2082, 2501, 2676
MluCI AATT 6 cut(s) 273, 790, 874, 1429, 2233, 2551
MlyI GAGTC 1 cut(s) 2118
MmeI TCCRAC 3 cut(s) 708, 1798, 2024
MroXI GAANNNNTTC 1 cut(s) 1929
MslI CAYNNNNRTG 1 cut(s) 2020
MspA1I CMGCKG 2 cut(s) 1162, 2568
MspI CCGG 6 cut(s) 941, 1200, 1804, 1922, 1939, 2667
MspR9I CCNGG 8 cut(s) 145, 942, 1287, 1523, 1646, 1782, 1940, 2444
MvaI CCWGG 6 cut(s) 145, 1287, 1523, 1646, 1782, 2444
MwoI GCNNNNNNNGC 3 cut(s) 425, 967, 2036
NciI CCSGG 2 cut(s) 942, 1940
NmuCI GTSAC 1 cut(s) 1949
NspI RCATGY 4 cut(s) 1152, 1468, 1894, 2623
NspV TTCGAA 2 cut(s) 224, 812
PasI CCCWGGG 1 cut(s) 1522
PciI ACATGT 1 cut(s) 1464
PdmI GAANNNNTTC 1 cut(s) 1929
PfeI GAWTC 5 cut(s) 149, 755, 1088, 2322, 2488
PflFI GACNNNGTC 1 cut(s) 595
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 1938
PkrI GCNGC 8 cut(s) 184, 379, 934, 1840, 2143, 2146, 2299, 2529
PleI GAGTC 1 cut(s) 2117
PpsI GAGTC 1 cut(s) 2117
PpuMI RGGWCCY 1 cut(s) 2247
PscI ACATGT 1 cut(s) 1464
PshBI ATTAAT 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 2247
Psp6I CCWGG 6 cut(s) 143, 1285, 1521, 1644, 1780, 2442
PspFI CCCAGC 4 cut(s) 428, 659, 733, 835
PspGI CCWGG 6 cut(s) 143, 1285, 1521, 1644, 1780, 2442
PspPI GGNCC 7 cut(s) 482, 529, 1269, 2051, 2229, 2247, 2263
PspPPI RGGWCCY 1 cut(s) 2247
PsrI GAACNNNNNNTAC 2 cut(s) 2639, 2671
PstNI CAGNNNCTG 2 cut(s) 884, 2036
PsuI RGATCY 4 cut(s) 1789, 1912, 2032, 2452
PsyI GACNNNGTC 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 1162
RseI CAYNNNNRTG 1 cut(s) 2020
SatI GCNGC 8 cut(s) 183, 378, 933, 1839, 2142, 2145, 2298, 2528
Sau96I GGNCC 7 cut(s) 482, 529, 1269, 2051, 2229, 2247, 2263
ScaI AGTACT 3 cut(s) 1883, 2258, 2648
SchI GAGTC 1 cut(s) 2118
ScrFI CCNGG 8 cut(s) 145, 942, 1287, 1523, 1646, 1782, 1940, 2444
SduI GDGCHC 8 cut(s) 51, 616, 668, 1522, 1675, 2082, 2501, 2676
SfcI CTRYAG 1 cut(s) 153
SfuI TTCGAA 2 cut(s) 224, 812
SinI GGWCC 6 cut(s) 482, 529, 1269, 2051, 2229, 2247
SmiMI CAYNNNNRTG 1 cut(s) 2020
SmlI CTYRAG 3 cut(s) 1255, 1528, 2181
SmoI CTYRAG 3 cut(s) 1255, 1528, 2181
SpeI ACTAGT 2 cut(s) 653, 1442
Sse9I AATT 6 cut(s) 273, 790, 874, 1429, 2233, 2551
SsiI CCGC 7 cut(s) 14, 182, 455, 1030, 2145, 2210, 2566
SspI AATATT 1 cut(s) 65
SspMI CTAG 9 cut(s) 134, 240, 654, 981, 1113, 1443, 2127, 2448, 2678
StyD4I CCNGG 8 cut(s) 143, 940, 1285, 1521, 1644, 1780, 1938, 2442
StyI CCWWGG 6 cut(s) 207, 299, 360, 1387, 1667, 2126
TaaI ACNGT 8 cut(s) 390, 706, 1269, 1297, 1579, 2229, 2288, 2358
TaiI ACGT 2 cut(s) 698, 2318
TaqI TCGA 7 cut(s) 82, 224, 628, 812, 889, 1469, 2107
TasI AATT 6 cut(s) 273, 790, 874, 1429, 2233, 2551
TatI WGTACW 8 cut(s) 447, 673, 1151, 1601, 1881, 2057, 2256, 2646
TauI GCSGC 2 cut(s) 185, 2147
TfiI GAWTC 5 cut(s) 149, 755, 1088, 2322, 2488
TscAI CASTG 1 cut(s) 1039
TseFI GTSAC 1 cut(s) 1949
TseI GCWGC 6 cut(s) 377, 932, 1838, 2141, 2297, 2527
Tsp45I GTSAC 1 cut(s) 1949
TspGWI ACGGA 4 cut(s) 1484, 2043, 2516, 2523
TspRI CASTG 1 cut(s) 1039
Tth111I GACNNNGTC 1 cut(s) 595
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 612
VpaK11BI GGWCC 6 cut(s) 482, 529, 1269, 2051, 2229, 2247
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 1 cut(s) 790
XceI RCATGY 4 cut(s) 1152, 1468, 1894, 2623
XcmI CCANNNNNNNNNTGG 2 cut(s) 1219, 1627
XmaJI CCTAGG 1 cut(s) 2126
XmnI GAANNNNTTC 1 cut(s) 1929
XspI CTAG 9 cut(s) 134, 240, 654, 981, 1113, 1443, 2127, 2448, 2678
ZrmI AGTACT 3 cut(s) 1883, 2258, 2648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.