MD12G1096800.v1.1
MYB Family

Myb/SANT-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013052
Physical Location & Seq
Forward (+)
15223375 .. 15225082
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1096800.v1.1.491

Sequence Viewer

Length: 1356 bp
ATGGAAGCCAATGGGTTGTTTTCTGGAATGCTTGGCCTGGAAATGCCTTTGCACCAAAACCCCCAAAACCCTCACCACCTTATGCACCAACCCCAAATGGTTGCCTATGCCCCCCACCATGACCCCGACAACCACCAAAACCCTCACCAATCTGTGAAACAGAGGCACCCATTTGCCCCCAAATCCAAACAAATTGCCCTCAGTGATGATGACGAACCCGGTTTCGGTGCTGATGACAACAGCAATGGGGATAACAAGAAGAAGATATCGCCATGGCAGAGAATGAAGTGGACCGACACCATGAAGCCTGCTGGTGGGTTGCTGCAGAAGAAAGGGAAGTGGAAATCGGTGTCCCGAGCTATGATGGAGAAGGGTTTCTACGTGTCGCCACAGCAATGTGAGGACAAGTTCAATGATTTGAATAAGAGGTATAAGAGGGTTAATGATATTCTTGGAAAGGGGACAGCTTGCCGGGTTGTGGAGAATCAGGGCTTGTTGGAAAAGATGGATTTGGCACCAAAGATGAAGGAAGAAGTTCGTAAATTGCTGAATTCGAAGCACCTGTTTTTCAGAGAAATGTGTGCTTATCATAATAGTTGTGGTCATGAAACTGTTGGTGGTCGGTCTAATGGCGCGCATAACTCCCCGCCACAGGTGGCCACAGAGCCGTCTGAGATACAAGTGTGGCCGCAGCCGCACGAGCAGCTCCAGCAGCAGAGATGCTTTCATTCATCAGAAAATGCTCAAGCGGTTGCCAATTCAGGAAGGTCAGAGACCAAGGGATCCAAGGTAATAAAAGGAGGAAGTGGCTGCGAGGACGAAGATGAAGAATTCGAGGATGATGATTCGGATGACGAGGTGGTTGGAGGTGGTTCTAGAGGTCACATTGGGCATGGAAATCACGAAGACAACGATGACAACGATGACAACGATGAAAGAGCTCCCCAGAAAGTGCCGAGAAAGGAAGGCTGTTTTTCGGGGTCATCCTCATCCACCCATTTAATGCAGCAATTGGGCTGTGAGGTGAGGGTTGTGCTGCAAGATGTGTCAAAGACCCCGTGGGAGAAGAAGCAGTGGTTGAAAACAAGGATAATAAAATTGCAGGAACAGCAAGTGAGCTACCAACACCGAGCTTTCGAGCTCGAGAAGCAGAGGCTCAAGTGGATCAAGTACAGTGGGAAGAAGGAAAGGTACATGGAGAGACGCAAGCTTGAGAACGAGAGAAGGCGGCTTGAGAACGAGAGGATGTTGTTGGTCGTCCGCGGGAAGGAGTTTGATTTGCTGGAGCTTCATCACCAGCAGCAGCAACAGAATTCTTCAAACAAAAGAAGTGATCCATCTTCCCTCACTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-bind_4

Protein Analysis

452

Amino Acids

51.64

Weight (kDa)

7.35

Isoelectric Point (pI)

55.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 105 - 160 2.9e-12 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10040
fragaria_vesca FvH4_6g17470
malus_domestica MD12G1096800.v1.1 MD14G1094300.v1.1
prunus_persica Prupe.7G120800_v2.0.a1
pyrus_communis pycom12g09810 pycom14g09210
rosa_chinensis RchiOBHm_Chr3g0471711
rosa_laevigata RLG00000024140
rosa_multiflora Rmu_sc0005631.1_g000025 Rmu_sc0026537.1_g000004
rosa_roxburghii Rroxscaffold_6G00409620
rosa_rugosa Rorug03G0118900
rosa_samantha Rh3AG170500 Rh3BG196200 Rh3CG186900 Rh3DG178800
rosa_wichuraiana Rw0G009030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 165, 514
AccII CGCG 2 cut(s) 635, 1263
AciI CCGC 7 cut(s) 647, 689, 695, 749, 1228, 1261, 1263
AclWI GGATC 4 cut(s) 777, 790, 1172, 1328
AcoI YGGCCR 2 cut(s) 657, 686
AcsI RAATTY 3 cut(s) 550, 830, 1312
AfaI GTAC 2 cut(s) 1172, 1193
AfiI CCNNNNNNNGG 5 cut(s) 224, 314, 478, 652, 1267
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 4 cut(s) 412, 421, 1081, 1320
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 9 cut(s) 359, 467, 706, 941, 1119, 1133, 1141, 1210, 1288
AluI AGCT 9 cut(s) 359, 467, 706, 941, 1119, 1133, 1141, 1210, 1288
Alw21I GWGCWC 2 cut(s) 943, 1143
Alw26I GTCTC 2 cut(s) 767, 1195
AlwI GGATC 4 cut(s) 777, 790, 1172, 1328
Ama87I CYCGRG 2 cut(s) 354, 1142
AoxI GGCC 3 cut(s) 34, 657, 686
ApeKI GCWGC 9 cut(s) 322, 691, 703, 712, 810, 1006, 1036, 1300, 1303
ApoI RAATTY 3 cut(s) 550, 830, 1312
Asp700I GAANNNNTTC 2 cut(s) 374, 534
AspLEI GCGC 2 cut(s) 635, 637
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 219, 473
AsuHPI GGTGA 4 cut(s) 65, 137, 1036, 1286
AsuII TTCGAA 1 cut(s) 554
AvaI CYCGRG 2 cut(s) 354, 1142
AvaII GGWCC 1 cut(s) 291
BaeI ACNNNNGTAYC 2 cut(s) 668, 701
BalI TGGCCA 1 cut(s) 659
BamHI GGATCC 1 cut(s) 782
BanI GGYRCC 2 cut(s) 165, 514
BanII GRGCYC 2 cut(s) 943, 1143
BarI GAAGNNNNNNTAC 4 cut(s) 362, 394, 1175, 1207
BauI CACGAG 1 cut(s) 698
BbsI GAAGAC 1 cut(s) 912
Bbv12I GWGCWC 2 cut(s) 943, 1143
BbvI GCAGC 9 cut(s) 309, 703, 715, 724, 797, 1018, 1023, 1312, 1315
BccI CCATC 3 cut(s) 358, 499, 1345
BceAI ACGGC 1 cut(s) 652
BciT130I CCWGG 1 cut(s) 38
BcnI CCSGG 2 cut(s) 219, 473
BcoDI GTCTC 2 cut(s) 767, 1195
BfaI CTAG 1 cut(s) 876
BfmI CTRYAG 1 cut(s) 323
Bme1390I CCNGG 3 cut(s) 38, 219, 473
Bme18I GGWCC 1 cut(s) 291
BmeT110I CYCGRG 2 cut(s) 354, 1142
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 3 cut(s) 167, 516, 784
BmrFI CCNGG 3 cut(s) 38, 219, 473
BmsI GCATC 1 cut(s) 710
BpiI GAAGAC 1 cut(s) 912
BpmI CTGGAG 2 cut(s) 692, 1304
Bpu14I TTCGAA 1 cut(s) 554
BpuEI CTTGAG 4 cut(s) 729, 1142, 1232, 1253
BpuMI CCSGG 2 cut(s) 219, 473
BsaAI YACGTR 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 767
BsaJI CCNNGG 5 cut(s) 272, 777, 786, 1058, 1261
Bsc4I CCNNNNNNNGG 5 cut(s) 224, 314, 478, 652, 1267
Bse1I ACTGG 1 cut(s) 1354
Bse3DI GCAATG 2 cut(s) 250, 401
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 5 cut(s) 272, 777, 786, 1058, 1261
BseGI GGATG 5 cut(s) 844, 856, 983, 989, 1251
BseLI CCNNNNNNNGG 5 cut(s) 224, 314, 478, 652, 1267
BseMI GCAATG 2 cut(s) 250, 401
BseMII CTCAG 2 cut(s) 214, 663
BseNI ACTGG 1 cut(s) 1354
BsePI GCGCGC 1 cut(s) 633
BseXI GCAGC 9 cut(s) 309, 703, 715, 724, 797, 1018, 1023, 1312, 1315
Bsh1236I CGCG 2 cut(s) 635, 1263
BshFI GGCC 3 cut(s) 36, 659, 688
BshNI GGYRCC 2 cut(s) 165, 514
BsiHKAI GWGCWC 2 cut(s) 943, 1143
BsiHKCI CYCGRG 2 cut(s) 354, 1142
BsiSI CCGG 2 cut(s) 219, 472
BslFI GGGAC 2 cut(s) 337, 475
BslI CCNNNNNNNGG 5 cut(s) 224, 314, 478, 652, 1267
BsmAI GTCTC 2 cut(s) 767, 1195
BsmBI CGTCTC 1 cut(s) 1195
BsmFI GGGAC 2 cut(s) 337, 475
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 3 cut(s) 36, 659, 688
Bso31I GGTCTC 1 cut(s) 767
BsoBI CYCGRG 2 cut(s) 354, 1142
Bsp119I TTCGAA 1 cut(s) 554
Bsp1286I GDGCHC 2 cut(s) 943, 1143
Bsp143I GATC 3 cut(s) 782, 1164, 1333
Bsp19I CCATGG 1 cut(s) 272
BspACI CCGC 7 cut(s) 647, 689, 695, 749, 1228, 1261, 1263
BspANI GGCC 3 cut(s) 36, 659, 688
BspCNI CTCAG 2 cut(s) 213, 664
BspFNI CGCG 2 cut(s) 635, 1263
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 3 cut(s) 167, 516, 784
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 4 cut(s) 777, 790, 1172, 1328
BspT104I TTCGAA 1 cut(s) 554
BspT107I GGYRCC 2 cut(s) 165, 514
BspTNI GGTCTC 1 cut(s) 767
BsrDI GCAATG 2 cut(s) 250, 401
BsrI ACTGG 1 cut(s) 1354
BssECI CCNNGG 5 cut(s) 272, 777, 786, 1058, 1261
BssHII GCGCGC 1 cut(s) 633
BssMI GATC 3 cut(s) 782, 1164, 1333
BssSI CACGAG 1 cut(s) 698
BssT1I CCWWGG 3 cut(s) 272, 777, 786
Bst2BI CACGAG 1 cut(s) 698
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 613, 1175
BstBAI YACGTR 1 cut(s) 382
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 4 cut(s) 309, 469, 635, 1208
BstDEI CTNAG 2 cut(s) 200, 672
BstDSI CCRYGG 3 cut(s) 272, 1058, 1261
BstF5I GGATG 5 cut(s) 844, 856, 983, 989, 1251
BstFNI CGCG 2 cut(s) 635, 1263
BstHHI GCGC 2 cut(s) 635, 637
BstKTI GATC 3 cut(s) 785, 1167, 1336
BstMAI GTCTC 2 cut(s) 767, 1195
BstMBI GATC 3 cut(s) 782, 1164, 1333
BstMWI GCNNNNNNNGC 7 cut(s) 694, 700, 703, 709, 712, 1108, 1147
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 3 cut(s) 36, 217, 471
BstSFI CTRYAG 1 cut(s) 323
BstUI CGCG 2 cut(s) 635, 1263
BstV1I GCAGC 9 cut(s) 309, 703, 715, 724, 797, 1018, 1023, 1312, 1315
BstV2I GAAGAC 1 cut(s) 912
BstX2I RGATCY 1 cut(s) 782
BstYI RGATCY 1 cut(s) 782
BsuRI GGCC 3 cut(s) 36, 659, 688
BtgI CCRYGG 3 cut(s) 272, 1058, 1261
BtsCI GGATG 5 cut(s) 844, 856, 983, 989, 1251
BtsI GCAGTG 1 cut(s) 1079
BtsIMutI CAGTG 4 cut(s) 208, 1079, 1180, 1347
Cac8I GCNNGC 4 cut(s) 309, 469, 635, 1208
CciI TCATGA 1 cut(s) 604
CfoI GCGC 2 cut(s) 635, 637
Cfr13I GGNCC 1 cut(s) 291
Cfr42I CCGCGG 1 cut(s) 1264
CseI GACGC 1 cut(s) 1212
Csp6I GTAC 2 cut(s) 1171, 1192
CspCI CAANNNNNGTGG 2 cut(s) 1156, 1191
CviAII CATG 6 cut(s) 119, 273, 301, 605, 893, 1195
CviQI GTAC 2 cut(s) 1171, 1192
DdeI CTNAG 2 cut(s) 200, 672
DpnI GATC 3 cut(s) 784, 1166, 1335
DpnII GATC 3 cut(s) 782, 1164, 1333
EaeI YGGCCR 2 cut(s) 657, 686
Ecl136II GAGCTC 2 cut(s) 941, 1141
Eco130I CCWWGG 3 cut(s) 272, 777, 786
Eco24I GRGCYC 2 cut(s) 943, 1143
Eco31I GGTCTC 1 cut(s) 767
Eco32I GATATC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 291
Eco53kI GAGCTC 2 cut(s) 941, 1141
Eco88I CYCGRG 2 cut(s) 354, 1142
EcoICRI GAGCTC 2 cut(s) 941, 1141
EcoRI GAATTC 3 cut(s) 550, 830, 1312
EcoRII CCWGG 1 cut(s) 36
EcoRV GATATC 1 cut(s) 267
EcoT14I CCWWGG 3 cut(s) 272, 777, 786
EcoT38I GRGCYC 2 cut(s) 943, 1143
ErhI CCWWGG 3 cut(s) 272, 777, 786
Esp3I CGTCTC 1 cut(s) 1195
FaeI CATG 6 cut(s) 122, 276, 304, 608, 896, 1198
FaqI GGGAC 2 cut(s) 337, 475
FatI CATG 6 cut(s) 118, 272, 300, 604, 892, 1194
FauI CCCGC 2 cut(s) 654, 1256
FokI GGATG 5 cut(s) 851, 863, 970, 976, 1258
FriOI GRGCYC 2 cut(s) 943, 1143
FspBI CTAG 1 cut(s) 876
GlaI GCGC 2 cut(s) 634, 636
GsuI CTGGAG 2 cut(s) 692, 1304
HaeIII GGCC 3 cut(s) 36, 659, 688
HapII CCGG 2 cut(s) 219, 472
HgaI GACGC 1 cut(s) 1212
HhaI GCGC 2 cut(s) 635, 637
Hin1II CATG 6 cut(s) 122, 276, 304, 608, 896, 1198
Hin6I GCGC 2 cut(s) 633, 635
HinP1I GCGC 2 cut(s) 633, 635
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 2 cut(s) 484, 845
HpaII CCGG 2 cut(s) 219, 472
HphI GGTGA 4 cut(s) 65, 137, 1036, 1286
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 5 cut(s) 572, 673, 736, 772, 850
Hpy188III TCNNGA 7 cut(s) 24, 354, 605, 762, 876, 902, 1144
Hpy8I GTNNAC 1 cut(s) 291
HpyAV CCTTC 7 cut(s) 364, 520, 759, 959, 1177, 1218, 1261
HpyCH4III ACNGT 2 cut(s) 613, 1175
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 6 cut(s) 52, 85, 325, 1006, 1039, 1102
HpyF10VI GCNNNNNNNGC 7 cut(s) 694, 700, 703, 709, 712, 1108, 1147
HpyF3I CTNAG 2 cut(s) 200, 672
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 6 cut(s) 122, 276, 304, 608, 896, 1198
HspAI GCGC 2 cut(s) 633, 635
KspI CCGCGG 1 cut(s) 1264
Kzo9I GATC 3 cut(s) 782, 1164, 1333
LmnI GCTCC 3 cut(s) 711, 946, 1285
Lsp1109I GCAGC 9 cut(s) 309, 703, 715, 724, 797, 1018, 1023, 1312, 1315
LweI GCATC 1 cut(s) 710
MaeI CTAG 1 cut(s) 876
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 881
MalI GATC 3 cut(s) 784, 1166, 1335
MboI GATC 3 cut(s) 782, 1164, 1333
MfeI CAATTG 1 cut(s) 1010
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 2 cut(s) 943, 1143
MlsI TGGCCA 1 cut(s) 659
MluCI AATT 8 cut(s) 192, 542, 550, 757, 830, 1010, 1097, 1312
MluNI TGGCCA 1 cut(s) 659
MmeI TCCRAC 2 cut(s) 477, 844
Mox20I TGGCCA 1 cut(s) 659
MroXI GAANNNNTTC 2 cut(s) 374, 534
MscI TGGCCA 1 cut(s) 659
MseI TTAA 2 cut(s) 441, 1001
MslI CAYNNNNRTG 1 cut(s) 394
Msp20I TGGCCA 1 cut(s) 659
MspA1I CMGCKG 1 cut(s) 1263
MspI CCGG 2 cut(s) 219, 472
MspR9I CCNGG 3 cut(s) 38, 219, 473
MunI CAATTG 1 cut(s) 1010
Mva1269I GAATGC 1 cut(s) 33
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 2 cut(s) 635, 1263
MwoI GCNNNNNNNGC 7 cut(s) 694, 700, 703, 709, 712, 1108, 1147
NciI CCSGG 2 cut(s) 219, 473
NcoI CCATGG 1 cut(s) 272
NdeII GATC 3 cut(s) 782, 1164, 1333
NlaIII CATG 6 cut(s) 122, 276, 304, 608, 896, 1198
NlaIV GGNNCC 3 cut(s) 167, 516, 784
NmeAIII GCCGAG 1 cut(s) 981
NmuCI GTSAC 1 cut(s) 881
NspV TTCGAA 1 cut(s) 554
PaeR7I CTCGAG 1 cut(s) 1142
PagI TCATGA 1 cut(s) 604
PauI GCGCGC 1 cut(s) 633
PcsI WCGNNNNNNNCGW 3 cut(s) 909, 918, 927
PctI GAATGC 1 cut(s) 33
PdmI GAANNNNTTC 2 cut(s) 374, 534
PfeI GAWTC 2 cut(s) 484, 845
Ppu21I YACGTR 1 cut(s) 382
Psp124BI GAGCTC 2 cut(s) 943, 1143
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 3 cut(s) 167, 516, 784
PspPI GGNCC 1 cut(s) 291
PstI CTGCAG 1 cut(s) 327
PsuI RGATCY 1 cut(s) 782
PteI GCGCGC 1 cut(s) 633
RsaI GTAC 2 cut(s) 1172, 1193
RsaNI GTAC 2 cut(s) 1171, 1192
RseI CAYNNNNRTG 1 cut(s) 394
SacI GAGCTC 2 cut(s) 943, 1143
SacII CCGCGG 1 cut(s) 1264
SaqAI TTAA 2 cut(s) 441, 1001
Sau3AI GATC 3 cut(s) 782, 1164, 1333
Sau96I GGNCC 1 cut(s) 291
ScrFI CCNGG 3 cut(s) 38, 219, 473
SduI GDGCHC 2 cut(s) 943, 1143
SfaNI GCATC 1 cut(s) 710
SfcI CTRYAG 1 cut(s) 323
Sfr274I CTCGAG 1 cut(s) 1142
Sfr303I CCGCGG 1 cut(s) 1264
SfuI TTCGAA 1 cut(s) 554
SgrBI CCGCGG 1 cut(s) 1264
SinI GGWCC 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 1142
SmiMI CAYNNNNRTG 1 cut(s) 394
SmlI CTYRAG 5 cut(s) 744, 1142, 1157, 1211, 1232
SmoI CTYRAG 5 cut(s) 744, 1142, 1157, 1211, 1232
Sse9I AATT 8 cut(s) 192, 542, 550, 757, 830, 1010, 1097, 1312
SsiI CCGC 7 cut(s) 647, 689, 695, 749, 1228, 1261, 1263
SspMI CTAG 1 cut(s) 876
SstI GAGCTC 2 cut(s) 943, 1143
StyD4I CCNGG 3 cut(s) 36, 217, 471
StyI CCWWGG 3 cut(s) 272, 777, 786
TaaI ACNGT 2 cut(s) 613, 1175
TaiI ACGT 1 cut(s) 384
TaqI TCGA 4 cut(s) 554, 834, 1137, 1143
TaqII GACCGA 2 cut(s) 308, 612
TasI AATT 8 cut(s) 192, 542, 550, 757, 830, 1010, 1097, 1312
TatI WGTACW 1 cut(s) 1170
TauI GCSGC 3 cut(s) 691, 697, 1231
TfiI GAWTC 2 cut(s) 484, 845
Tru1I TTAA 2 cut(s) 441, 1001
Tru9I TTAA 2 cut(s) 441, 1001
TscAI CASTG 4 cut(s) 208, 1079, 1180, 1354
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 9 cut(s) 322, 691, 703, 712, 810, 1006, 1036, 1300, 1303
Tsp45I GTSAC 1 cut(s) 881
TspDTI ATGAA 9 cut(s) 299, 317, 539, 621, 716, 720, 840, 948, 1280
TspRI CASTG 4 cut(s) 208, 1079, 1180, 1354
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 3 cut(s) 550, 830, 1312
XbaI TCTAGA 1 cut(s) 875
XhoI CTCGAG 1 cut(s) 1142
XmnI GAANNNNTTC 2 cut(s) 374, 534
XspI CTAG 1 cut(s) 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.