MD12G1099300.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018896
Physical Location & Seq
Forward (+)
15469052 .. 15469871
820 bp
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UTR
Exon/CDS
Intron
MD12G1099300.v1.1.491

Sequence Viewer

Length: 663 bp
ATGTGTGGTCCGGTGCAGGAAATATTTCGGGCTGGTTTGGAAACAACAAGCAGCACCACAGAATGGGCATTGACCGAGCTGTTATGCAACCCAGCAACACTAATTGAGGCGAAAGCCGAGCTCACTCAAGTAATATGTCCGAGCAGAAAGATTGAAGAGAGTGATATTGACAATCTTCCATACTTGCAGGGCATAATCAACGAAACGCTAAGATTGCATCCACCAATTCCATTCCTAGTACCAAGAAAGGCAATGCATGACACCAGTTTCATGGGGTACTTTGCACCCAAAGACACACAAGTGTTTGTGAATGCTTATGCAATTGGAAGAGATCGAGATGTGTGGCGTGAAGAGCCGATGTCGTCTAAGCCCGAGAGGTTCGTAGTGTTAGGAGTCTGTGACAGATGGCATAATTCTGTTGGTTCTGTTGGTAATAGTTGGATGAGAGTTAGTTGGAATTATGAGTTGATTCCATTTGGAGCTGGGAGAAGAATGTGTGCAGGTATTCCTCTGGCTCATAGAATGCTGCATCTTACATTGGGGATGTTGCTTCAACGATTTGATTGGTCACTTGACGCAAATGTTACTAGAGAAACTATGGATTGGAAGGACAAGTTGGGGATAACAATGACAAAATCTGAGCCATTGCTAGCAGTGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

221

Amino Acids

24.98

Weight (kDa)

5.97

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 5 - 213 1.7e-55 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018896)

Species Orthologous Gene IDs
malus_domestica MD12G1099300.v1.1 MD16G1115700.v1.1
rosa_chinensis RchiOBHm_Chr1g0346031 RchiOBHm_Chr4g0430061 RchiOBHm_Chr7g0218011
rosa_laevigata RLG00000024131
rosa_roxburghii Rroxscaffold_5G00360940
rosa_rugosa Rorug03G0120100 Rorug03G0120400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 491
AccB7I CCANNNNNTGG 1 cut(s) 63
AfaI GTAC 2 cut(s) 240, 278
AfiI CCNNNNNNNGG 1 cut(s) 63
AgsI TTSAA 2 cut(s) 155, 554
AjuI GAANNNNNNNTTGG 2 cut(s) 599, 631
AleI CACNNNNGTG 1 cut(s) 299
AluBI AGCT 3 cut(s) 79, 121, 482
AluI AGCT 3 cut(s) 79, 121, 482
Alw21I GWGCWC 1 cut(s) 123
Ama87I CYCGRG 1 cut(s) 371
ApeKI GCWGC 2 cut(s) 51, 526
Asp700I GAANNNNTTC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 8
AsuNHI GCTAGC 1 cut(s) 649
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 63, 513
BccI CCATC 1 cut(s) 399
BfaI CTAG 3 cut(s) 236, 588, 650
BfuAI ACCTGC 1 cut(s) 491
BisI GCNGC 2 cut(s) 52, 527
BlsI GCNGC 2 cut(s) 53, 528
Bme18I GGWCC 1 cut(s) 8
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 8
BmsI GCATC 2 cut(s) 226, 538
BmtI GCTAGC 1 cut(s) 653
BpuEI CTTGAG 1 cut(s) 111
BsaWI WCCGGW 1 cut(s) 10
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse1I ACTGG 1 cut(s) 264
Bse3DI GCAATG 2 cut(s) 258, 644
BseGI GGATG 3 cut(s) 217, 447, 549
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMI GCAATG 2 cut(s) 258, 644
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 264
BseXI GCAGC 2 cut(s) 63, 513
BseYI CCCAGC 2 cut(s) 91, 482
BsgI GTGCAG 2 cut(s) 35, 519
BsiHKAI GWGCWC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 11
BslI CCNNNNNNNGG 1 cut(s) 63
BsmI GAATGC 2 cut(s) 316, 528
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 1 cut(s) 331
BspCNI CTCAG 1 cut(s) 631
BspMI ACCTGC 1 cut(s) 491
BspOI GCTAGC 1 cut(s) 653
BspQI GCTCTTC 1 cut(s) 345
BsrDI GCAATG 2 cut(s) 258, 644
BsrI ACTGG 1 cut(s) 264
BssMI GATC 1 cut(s) 331
Bst6I CTCTTC 3 cut(s) 150, 322, 345
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 4 cut(s) 209, 366, 639, 660
BstF5I GGATG 3 cut(s) 217, 447, 549
BstKTI GATC 1 cut(s) 334
BstMBI GATC 1 cut(s) 331
BstMWI GCNNNNNNNGC 3 cut(s) 214, 352, 655
BstV1I GCAGC 2 cut(s) 63, 513
BstXI CCANNNNNNTGG 1 cut(s) 271
BtsCI GGATG 3 cut(s) 217, 447, 549
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 1 cut(s) 660
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 1 cut(s) 651
Cfr13I GGNCC 1 cut(s) 8
CseI GACGC 1 cut(s) 584
Csp6I GTAC 2 cut(s) 239, 277
CviAII CATG 2 cut(s) 257, 271
CviJI RGCY 9 cut(s) 32, 79, 116, 121, 355, 370, 482, 515, 643
CviKI_1 RGCY 9 cut(s) 32, 79, 116, 121, 355, 370, 482, 515, 643
CviQI GTAC 2 cut(s) 239, 277
DdeI CTNAG 4 cut(s) 209, 366, 639, 660
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
Eam1104I CTCTTC 3 cut(s) 150, 322, 345
EarI CTCTTC 3 cut(s) 150, 322, 345
Ecl136II GAGCTC 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 123
FaeI CATG 2 cut(s) 260, 274
FatI CATG 2 cut(s) 256, 270
Fnu4HI GCNGC 2 cut(s) 52, 527
FokI GGATG 3 cut(s) 204, 454, 556
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 2 cut(s) 52, 527
FspBI CTAG 3 cut(s) 236, 588, 650
GluI GCNGC 2 cut(s) 52, 527
GsaI CCCAGC 2 cut(s) 95, 486
HapII CCGG 1 cut(s) 11
HgaI GACGC 1 cut(s) 584
Hin1II CATG 2 cut(s) 260, 274
HinfI GANTC 2 cut(s) 393, 469
HpaII CCGG 1 cut(s) 11
Hpy188I TCNGA 2 cut(s) 141, 640
Hpy188III TCNNGA 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 601
HpyCH4V TGCA 9 cut(s) 16, 87, 187, 217, 256, 284, 320, 500, 529
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 352, 655
HpyF3I CTNAG 4 cut(s) 209, 366, 639, 660
Hsp92II CATG 2 cut(s) 260, 274
Kzo9I GATC 1 cut(s) 331
LguI GCTCTTC 1 cut(s) 345
LmnI GCTCC 1 cut(s) 479
LpnPI CCDG 9 cut(s) 2, 18, 24, 105, 173, 277, 468, 486, 497
Lsp1109I GCAGC 2 cut(s) 63, 513
LweI GCATC 2 cut(s) 226, 538
MaeI CTAG 3 cut(s) 236, 588, 650
MaeIII GTNAC 3 cut(s) 398, 567, 583
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 5 cut(s) 167, 167, 339, 362, 501
MfeI CAATTG 1 cut(s) 321
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 5 cut(s) 102, 225, 321, 412, 457
MlyI GAGTC 1 cut(s) 402
MmeI TCCRAC 2 cut(s) 419, 434
MnlI CCTC 3 cut(s) 100, 369, 519
Mph1103I ATGCAT 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 24
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 1 cut(s) 11
MunI CAATTG 1 cut(s) 321
Mva1269I GAATGC 2 cut(s) 316, 528
MwoI GCNNNNNNNGC 3 cut(s) 214, 352, 655
NdeII GATC 1 cut(s) 331
NheI GCTAGC 1 cut(s) 649
NlaIII CATG 2 cut(s) 260, 274
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 2 cut(s) 398, 567
NsiI ATGCAT 1 cut(s) 258
OliI CACNNNNGTG 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 345
PctI GAATGC 2 cut(s) 316, 528
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 469
PflMI CCANNNNNTGG 1 cut(s) 63
PkrI GCNGC 2 cut(s) 53, 528
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
Psp124BI GAGCTC 1 cut(s) 123
PspFI CCCAGC 2 cut(s) 91, 482
PspPI GGNCC 1 cut(s) 8
RsaI GTAC 2 cut(s) 240, 278
RsaNI GTAC 2 cut(s) 239, 277
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 123
SapI GCTCTTC 1 cut(s) 345
SatI GCNGC 2 cut(s) 52, 527
Sau3AI GATC 1 cut(s) 331
Sau96I GGNCC 1 cut(s) 8
SchI GAGTC 1 cut(s) 402
SduI GDGCHC 1 cut(s) 123
SetI ASST 5 cut(s) 81, 123, 380, 484, 505
SfaNI GCATC 2 cut(s) 226, 538
SinI GGWCC 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 126
SmoI CTYRAG 1 cut(s) 126
Sse9I AATT 5 cut(s) 102, 225, 321, 412, 457
SspI AATATT 1 cut(s) 24
SspMI CTAG 3 cut(s) 236, 588, 650
SstI GAGCTC 1 cut(s) 123
TaqI TCGA 1 cut(s) 334
TaqII GACCGA 1 cut(s) 89
TasI AATT 5 cut(s) 102, 225, 321, 412, 457
TfiI GAWTC 1 cut(s) 469
TscAI CASTG 1 cut(s) 660
TseFI GTSAC 2 cut(s) 398, 567
TseI GCWGC 2 cut(s) 51, 526
Tsp45I GTSAC 2 cut(s) 398, 567
TspDTI ATGAA 1 cut(s) 259
TspRI CASTG 1 cut(s) 660
Van91I CCANNNNNTGG 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 3 cut(s) 236, 588, 650
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.