Rorug03G0120100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0018896
Physical Location & Seq
Reverse (-)
9682207 .. 9685500
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0120100.1

Sequence Viewer

Length: 3159 bp
ATGAAGGGGCATTCACTATCAAATTGTAGGTGGTTTTTACACAAATTCCTTCCTGTGTTCATTGTTTTATGCATAAGCACAGGTCTAGAATCTGCAACACTACCAAATTTTGGAAATGAATCTGATAGTCTGGCACTGCTAGACTTCAAGAAAAGAATCACTGAAGATCCTCTCCATATCATGAGCTCATGGAATGAATCCATGCATTTCTGCAGTTGGATAGGCGTCACATGCAACAATTCCACCAGAAGAGTCGTGACTTTGAACTTGCAAGCTCAAAAGTTGGCTGGCTCCGTACCACCTTCTATAGGGAATCTCACTTATCTCACTGGAATCAACTTGATAAACAACAACTTTTATGGTGAAATTCCTCAGGAAATGGGTCGTCTCTTGCGCCTGCAATATCTCAACATGTCTCAAAACTCCTTCGGTGCGAAAATTCCAACTAATATATCTCACTGTACGCAGCTGACAATGATTGATGTTTTTTCCAATAAACTCATTGGGCCAATTCCCGACCAACTCAGTTCATTGTTGAAATTAACTGTGTTATGGATTGGTCGGAACAATCTCACTGGAACCATCCCACGTTGGATTGGGAACTTTTCTTCATTGTTTGCACTTTCTCTTGGTGAAAACAACTTGCAAGGAAGCATACCCAATGAGCTGGGGCGTCTAACTGCTTTGCAGAGATTCATACTTACCTCAAATAATCTTTCTGGTATGATTCCTTCTTCGATATATAACATTTCCTCCATATCCTATTTCACTGTTACTCAGAATCAACTGCATGGGGAAATCCCACAAAACATTGGCATTACTCTTCCTAATCTGGAGGTATTTGCCGGAGGTGCCAACAAGTTCACAGGAACTATTCCTGTGTCCTTGTCAAATGCTTCTCAGCTATCCATTCTTGAATTTTCCCGAAATAGTTTTACTGGGAAACTCCCTGCTGAAAGTCTTGGGCGTTTGAAAAGGTTAGTTAGACTATACTTTGACTTAAACAGACTGGGAAGTGGTCAAGCTGGTGGCCTGAATTTTCTCAATTTCCTGACTAATTGTACTAGTCTTGAGGTGTTGGGTCTTAGCTATAATCGCTTTGGAGGAGAATTACCAGCATCCATAGCCAACCTTTCTACCCAGCTAAAAGTTCTTACTCTGGGGGGAAATTTGATACATGGAAGCCTCCCTACTGGCATTGGAAATCTGGTAAACTTGACACTTCTGGGAATAGAAGGTAACCACATATCCGGTAGTCTCCCTGATGCAATCGGGAAGCTTCACAAGTTAGAAGGACTGCATTTGAATCTGAACAGATTTTCAGGGCCAATCCCATTCTCCCTAGGTAACTTGACTTCAGTGACAAGGCTTTTCATGGAGGGGAATAGGTTTCAGGGAAGCATACCTCCAAGTCTTGGGAACTGCCAAAATCTATTGATACTCAACCTTTCTAGTAACAATCTAAGTAGCACAATACCTAGAGAGGTAGTGGAGATTAAATCCCTTTCGATTTCTTTGACAATGTCTAACAACAATTTGACTGGTTCACTACCATCTGAAGTGGGTGGTTTGGTAAATCTAGCAGAGCTAGACATATCAGGAAACAAGTTATCAGGTGAAATCCCCGAAACCCTTGGCAGTTGTATTAGTTTGGGGCGCCTGATTTTGGAAGGTAATGAATTCAGTGGACATATTCCTCAGTCTATGGAAAAGCTGAGAGGCTTGGAAGAGTTGGATATTTCACGCAATAACTTATCTGGCCAGGTTCCTAAATTTGTAGGCAAGTTTCGAGCTCTCAAGTATCTCAATCTTTCTCACAATGATTTTGAGGGTGAATTGCCTAAAGAAGGAATCTTTTCAAATGCAACTGGTATCTCAGTGCTAGGAAATGATAAGCTCTGTGGTGGAATTCCAGAATTAGTTCTACCTGCATGTTCCAGCAGAAAGCCTCCTTCATCGAGAGGACTACTTGCCCCAAAAGTGATAATCCCTCTAACTAGTGCAATTGCATTAATAATTGCTCTTTCCTGCTTGGTTGCTGCACGTTCAATTATGAAAAAGCCAAGAGATGGACCTCTGACTTCATCTTCTCATAATGATTCATATCCAAGTATCTCTTACTCGGAACTTGTTCAGTCAACTAACGGGTTTTCTGTGGACAATTTGATTGGTTCAGGAAGCTTTGGTTCTGTATACAAAGGATTACTCAGTAGTACTGGAATGGTAGTTGCTGTTAAGGTATTAAACCTTCAACAACAAGGAGCTTCAAAGAGTTTCATTGATGAATGCAGAGCTCTAAGAAGTGTAAGGCACCGCAATCTTCTAAAGATCATAACTACCTGCTCAAGCACCGATAATCAGGGTAACGACTTCAAAAGTCTAGTTTTCAATTTCATGGCAAATGGAAGTCTAGATCCTTGGCTGCACCCTAGAGAAGATGACGGATCTCAAAGTAAGAGATTGAGCCTTATCCAAAGGCTCAATATTGCCATTGATGTTGCTTGCGCATTAGATTACCTACACAACGATTGTGAAATATCCATTGTTCATTGTGATCTAAAGCCAAGTAATGTCCTTCTCGATGAGTATATGGTAGCCCATGTTGGTGACTTTGGTTTAGCAAGCTTCCTCTTCGAAGCATCAAATAATCATTCCAAAAGTCAGACCATGTCAGCTAGGCTAAAGGGTTCCATAGGCTACATTCCTCCAGAATATGGCATGGGATGTCAAGTTTCCATTCTGGGAGACATCTATAGCTATGGGATTCTATTGCTAGAAATGTTCACAGGGAAGAGGCCAACGGACGAAATGTTCAAAGATGGTCTAAGCATTCACCAATTCACAGCCATGGCTTTCCCTGACCGTGTCATGGACATAGTTGACACTTCATTGCTCCTGGAAACAGATGAGGATAAGGATAATGACGATGATGACAAATACAGCAATGATATACAAGAAAGGCCAATAGCCGGGTATCAAGATATAGGCCTAGTCAAAGCAAGAAGATTGGAGGAATGCTTGGTTTCAGTGATGCAGATCGGTCTCTCATGCTCCACAGTATCACCAAGTGAGCGCATTCTCATGAATGTGGTTGTGAACAAGATGACTGCAATTAGAGATTCCTATCTCAAATTGACCAAGACCAAGTTTGTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

1052

Amino Acids

114.56

Weight (kDa)

6.86

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 40 - 79 6.6e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 125 - 333 1.4e-12 Leucine-rich repeat region
LRR_8 PF13855 180 - 239 5.5e-07 Leucine rich repeat
LRR_14 PF23598 350 - 490 1.1e-09 Leucine-rich repeat region
LRR_14 PF23598 468 - 607 1.2e-08 Leucine-rich repeat region
LRR_8 PF13855 550 - 609 5e-07 Leucine rich repeat
Pkinase PF00069 718 - 941 1.7e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 719 - 939 5.6e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018896)

Species Orthologous Gene IDs
malus_domestica MD12G1099300.v1.1 MD16G1115700.v1.1
rosa_chinensis RchiOBHm_Chr1g0346031 RchiOBHm_Chr4g0430061 RchiOBHm_Chr7g0218011
rosa_laevigata RLG00000024131
rosa_roxburghii Rroxscaffold_5G00360940
rosa_rugosa Rorug03G0120100 Rorug03G0120400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2506
Acc36I ACCTGC 2 cut(s) 1936, 2348
AccB1I GGYRCC 3 cut(s) 851, 1656, 2310
AccB7I CCANNNNNTGG 4 cut(s) 110, 1415, 2069, 2714
AccI GTMKAC 1 cut(s) 2193
AciI CCGC 1 cut(s) 2314
AclWI GGATC 3 cut(s) 161, 2408, 2452
AcoI YGGCCR 1 cut(s) 1759
AcuI CTGAAG 3 cut(s) 183, 1341, 1578
AcyI GRCGYC 3 cut(s) 225, 673, 1657
AdeI CACNNNGTG 1 cut(s) 3068
AfaI GTAC 4 cut(s) 297, 463, 1063, 2215
AfiI CCNNNNNNNGG 9 cut(s) 110, 308, 1415, 1666, 1847, 2069, 2714, 2869, 2969
AflIII ACRYGT 1 cut(s) 411
AhlI ACTAGT 2 cut(s) 1064, 1997
AjnI CCWGG 2 cut(s) 1761, 2895
AloI GAACNNNNNNTCC 2 cut(s) 2795, 2827
Alw21I GWGCWC 3 cut(s) 188, 1795, 2296
Alw26I GTCTC 5 cut(s) 392, 420, 1262, 2739, 3047
AlwI GGATC 3 cut(s) 161, 2408, 2452
AoxI GGCC 7 cut(s) 506, 1030, 1325, 1759, 2795, 2960, 2986
ApeKI GCWGC 3 cut(s) 466, 2039, 2422
AseI ATTAAT 1 cut(s) 2012
Asp700I GAANNNNTTC 3 cut(s) 1855, 1920, 2130
AspA2I CCTAGG 1 cut(s) 1342
AspLEI GCGC 4 cut(s) 396, 1659, 2507, 3075
AspS9I GGNCC 3 cut(s) 506, 1325, 2072
AsuC2I CCSGG 1 cut(s) 2971
AsuHPI GGTGA 8 cut(s) 374, 644, 1628, 1844, 2618, 2825, 3054, 3145
AsuII TTCGAA 1 cut(s) 2634
AvaII GGWCC 1 cut(s) 2072
AvrII CCTAGG 1 cut(s) 1342
AxyI CCTNAGG 1 cut(s) 372
BalI TGGCCA 1 cut(s) 1761
BanI GGYRCC 3 cut(s) 851, 1656, 2310
BanII GRGCYC 3 cut(s) 188, 1795, 2296
BarI GAAGNNNNNNTAC 8 cut(s) 715, 747, 1174, 1206, 1339, 1371, 2232, 2264
Bbv12I GWGCWC 3 cut(s) 188, 1795, 2296
BbvI GCAGC 3 cut(s) 478, 2026, 2409
BccI CCATC 4 cut(s) 590, 1561, 2063, 2813
BciT130I CCWGG 2 cut(s) 1763, 2897
BcnI CCSGG 1 cut(s) 2971
BcoDI GTCTC 5 cut(s) 392, 420, 1262, 2739, 3047
BcuI ACTAGT 2 cut(s) 1064, 1997
BfmI CTRYAG 3 cut(s) 211, 306, 2752
BfoI RGCGCY 1 cut(s) 1660
BfuAI ACCTGC 2 cut(s) 1936, 2348
BisI GCNGC 3 cut(s) 467, 2040, 2423
BlnI CCTAGG 1 cut(s) 1342
BlsI GCNGC 3 cut(s) 468, 2041, 2424
BmcAI AGTACT 1 cut(s) 2215
Bme1390I CCNGG 3 cut(s) 1763, 2897, 2971
Bme18I GGWCC 1 cut(s) 2072
BmgT120I GGNCC 3 cut(s) 506, 1325, 2072
BmiI GGNNCC 7 cut(s) 292, 580, 853, 1658, 1767, 2312, 2689
BmrFI CCNGG 3 cut(s) 1763, 2897, 2971
BmrI ACTGGG 2 cut(s) 948, 1019
BmsI GCATC 4 cut(s) 1127, 1255, 2648, 3021
BmuI ACTGGG 2 cut(s) 948, 1019
BpmI CTGGAG 2 cut(s) 854, 2691
Bpu14I TTCGAA 1 cut(s) 2634
BpuEI CTTGAG 3 cut(s) 1091, 1781, 2329
BpuMI CCSGG 1 cut(s) 2971
BsaBI GATNNNNATC 3 cut(s) 2103, 3035, 3123
BsaHI GRCGYC 3 cut(s) 225, 673, 1657
BsaI GGTCTC 1 cut(s) 3047
BsaJI CCNNGG 4 cut(s) 1342, 1633, 2417, 2847
BsaWI WCCGGW 1 cut(s) 1250
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bsc4I CCNNNNNNNGG 9 cut(s) 110, 308, 1415, 1666, 1847, 2069, 2714, 2869, 2969
Bse1I ACTGG 8 cut(s) 334, 580, 943, 1014, 1198, 1546, 1873, 2221
Bse21I CCTNAGG 1 cut(s) 372
Bse3DI GCAATG 2 cut(s) 2888, 2950
Bse8I GATNNNNATC 3 cut(s) 2103, 3035, 3123
BseBI CCWGG 2 cut(s) 1763, 2897
BseDI CCNNGG 4 cut(s) 1342, 1633, 2417, 2847
BseGI GGATG 3 cut(s) 582, 1118, 2729
BseJI GATNNNNATC 3 cut(s) 2103, 3035, 3123
BseLI CCNNNNNNNGG 9 cut(s) 110, 308, 1415, 1666, 1847, 2069, 2714, 2869, 2969
BseMI GCAATG 2 cut(s) 2888, 2950
BseMII CTCAG 8 cut(s) 386, 538, 791, 914, 1706, 1712, 1890, 2221
BseNI ACTGG 8 cut(s) 334, 580, 943, 1014, 1198, 1546, 1873, 2221
BseRI GAGGAG 1 cut(s) 1119
BseXI GCAGC 3 cut(s) 478, 2026, 2409
BseYI CCCAGC 2 cut(s) 667, 1140
BsgI GTGCAG 2 cut(s) 2025, 2408
BshFI GGCC 7 cut(s) 508, 1032, 1327, 1761, 2797, 2962, 2988
BshNI GGYRCC 3 cut(s) 851, 1656, 2310
BsiHKAI GWGCWC 3 cut(s) 188, 1795, 2296
BsiSI CCGG 3 cut(s) 846, 1251, 2970
BslI CCNNNNNNNGG 9 cut(s) 110, 308, 1415, 1666, 1847, 2069, 2714, 2869, 2969
BsmAI GTCTC 5 cut(s) 392, 420, 1262, 2739, 3047
BsmBI CGTCTC 1 cut(s) 392
BsmI GAATGC 5 cut(s) 10, 2291, 2829, 3020, 3075
BsnI GGCC 7 cut(s) 508, 1032, 1327, 1761, 2797, 2962, 2988
Bso31I GGTCTC 1 cut(s) 3047
Bsp119I TTCGAA 1 cut(s) 2634
Bsp1286I GDGCHC 3 cut(s) 188, 1795, 2296
Bsp143I GATC 6 cut(s) 166, 2328, 2413, 2444, 2554, 3036
Bsp19I CCATGG 1 cut(s) 2847
BspACI CCGC 1 cut(s) 2314
BspANI GGCC 7 cut(s) 508, 1032, 1327, 1761, 2797, 2962, 2988
BspCNI CTCAG 8 cut(s) 385, 537, 790, 913, 1707, 1711, 1889, 2220
BspHI TCATGA 2 cut(s) 180, 3081
BspLI GGNNCC 7 cut(s) 292, 580, 853, 1658, 1767, 2312, 2689
BspMAI CTGCAG 1 cut(s) 215
BspMI ACCTGC 2 cut(s) 1936, 2348
BspPI GGATC 3 cut(s) 161, 2408, 2452
BspT104I TTCGAA 1 cut(s) 2634
BspT107I GGYRCC 3 cut(s) 851, 1656, 2310
BspTNI GGTCTC 1 cut(s) 3047
BsrDI GCAATG 2 cut(s) 2888, 2950
BsrI ACTGG 8 cut(s) 334, 580, 943, 1014, 1198, 1546, 1873, 2221
BssECI CCNNGG 4 cut(s) 1342, 1633, 2417, 2847
BssMI GATC 6 cut(s) 166, 2328, 2413, 2444, 2554, 3036
BssNAI GTATAC 1 cut(s) 2194
BssNI GRCGYC 3 cut(s) 225, 673, 1657
BssT1I CCWWGG 4 cut(s) 1342, 1633, 2417, 2847
Bst1107I GTATAC 1 cut(s) 2194
Bst2UI CCWGG 2 cut(s) 1763, 2897
Bst4CI ACNGT 5 cut(s) 461, 547, 772, 2864, 3058
Bst6I CTCTTC 5 cut(s) 244, 828, 1722, 2636, 2786
BstACI GRCGYC 3 cut(s) 225, 673, 1657
BstBI TTCGAA 1 cut(s) 2634
BstC8I GCNNGC 5 cut(s) 273, 289, 398, 2503, 2623
BstDSI CCRYGG 1 cut(s) 2847
BstEII GGTNACC 1 cut(s) 1238
BstENI CCTNNNNNAGG 2 cut(s) 306, 1845
BstF5I GGATG 3 cut(s) 582, 1118, 2729
BstH2I RGCGCY 1 cut(s) 1660
BstHHI GCGC 4 cut(s) 396, 1659, 2507, 3075
BstKTI GATC 6 cut(s) 169, 2331, 2416, 2447, 2557, 3039
BstMAI GTCTC 5 cut(s) 392, 420, 1262, 2739, 3047
BstMBI GATC 6 cut(s) 166, 2328, 2413, 2444, 2554, 3036
BstMWI GCNNNNNNNGC 4 cut(s) 231, 851, 1095, 1124
BstNI CCWGG 2 cut(s) 1763, 2897
BstNSI RCATGY 3 cut(s) 234, 415, 1935
BstPI GGTNACC 1 cut(s) 1238
BstSCI CCNGG 3 cut(s) 1761, 2895, 2969
BstSFI CTRYAG 3 cut(s) 211, 306, 2752
BstV1I GCAGC 3 cut(s) 478, 2026, 2409
BstX2I RGATCY 3 cut(s) 166, 2413, 2444
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 3 cut(s) 166, 2413, 2444
BstZ17I GTATAC 1 cut(s) 2194
Bsu36I CCTNAGG 1 cut(s) 372
BsuRI GGCC 7 cut(s) 508, 1032, 1327, 1761, 2797, 2962, 2988
BtgI CCRYGG 1 cut(s) 2847
BtsCI GGATG 3 cut(s) 582, 1118, 2729
BtsI GCAGTG 1 cut(s) 134
BveI ACCTGC 2 cut(s) 1936, 2348
Cac8I GCNNGC 5 cut(s) 273, 289, 398, 2503, 2623
CciI TCATGA 2 cut(s) 180, 3081
CfoI GCGC 4 cut(s) 396, 1659, 2507, 3075
Cfr13I GGNCC 3 cut(s) 506, 1325, 2072
CseI GACGC 2 cut(s) 214, 662
Csp6I GTAC 4 cut(s) 296, 462, 1062, 2214
CviQI GTAC 4 cut(s) 296, 462, 1062, 2214
DinI GGCGCC 1 cut(s) 1658
DpnI GATC 6 cut(s) 168, 2330, 2415, 2446, 2556, 3038
DpnII GATC 6 cut(s) 166, 2328, 2413, 2444, 2554, 3036
DraIII CACNNNGTG 1 cut(s) 3068
EaeI YGGCCR 1 cut(s) 1759
Eam1104I CTCTTC 5 cut(s) 244, 828, 1722, 2636, 2786
EarI CTCTTC 5 cut(s) 244, 828, 1722, 2636, 2786
Ecl136II GAGCTC 3 cut(s) 186, 1793, 2294
Eco130I CCWWGG 4 cut(s) 1342, 1633, 2417, 2847
Eco147I AGGCCT 1 cut(s) 2988
Eco24I GRGCYC 3 cut(s) 188, 1795, 2296
Eco31I GGTCTC 1 cut(s) 3047
Eco47I GGWCC 1 cut(s) 2072
Eco53kI GAGCTC 3 cut(s) 186, 1793, 2294
Eco57I CTGAAG 3 cut(s) 183, 1341, 1578
Eco81I CCTNAGG 1 cut(s) 372
Eco91I GGTNACC 1 cut(s) 1238
EcoICRI GAGCTC 3 cut(s) 186, 1793, 2294
EcoNI CCTNNNNNAGG 2 cut(s) 306, 1845
EcoO65I GGTNACC 1 cut(s) 1238
EcoRI GAATTC 2 cut(s) 1679, 1908
EcoRII CCWGG 2 cut(s) 1761, 2895
EcoT14I CCWWGG 4 cut(s) 1342, 1633, 2417, 2847
EcoT22I ATGCAT 2 cut(s) 74, 207
EcoT38I GRGCYC 3 cut(s) 188, 1795, 2296
EgeI GGCGCC 1 cut(s) 1658
EheI GGCGCC 1 cut(s) 1658
ErhI CCWWGG 4 cut(s) 1342, 1633, 2417, 2847
Esp3I CGTCTC 1 cut(s) 392
FalI AAGNNNNNCTT 2 cut(s) 2101, 2133
FblI GTMKAC 1 cut(s) 2193
Fnu4HI GCNGC 3 cut(s) 467, 2040, 2423
FokI GGATG 3 cut(s) 569, 1105, 2736
FriOI GRGCYC 3 cut(s) 188, 1795, 2296
Fsp4HI GCNGC 3 cut(s) 467, 2040, 2423
FspI TGCGCA 1 cut(s) 2506
GlaI GCGC 4 cut(s) 395, 1658, 2506, 3074
GluI GCNGC 3 cut(s) 467, 2040, 2423
GsaI CCCAGC 2 cut(s) 671, 1144
GsuI CTGGAG 2 cut(s) 854, 2691
HaeII RGCGCY 1 cut(s) 1660
HaeIII GGCC 7 cut(s) 508, 1032, 1327, 1761, 2797, 2962, 2988
HapII CCGG 3 cut(s) 846, 1251, 2970
HgaI GACGC 2 cut(s) 214, 662
HhaI GCGC 4 cut(s) 396, 1659, 2507, 3075
Hin1I GRCGYC 3 cut(s) 225, 673, 1657
Hin6I GCGC 4 cut(s) 394, 1657, 2505, 3073
HinP1I GCGC 4 cut(s) 394, 1657, 2505, 3073
HincII GTYRAC 2 cut(s) 2139, 2881
HindII GTYRAC 2 cut(s) 2139, 2881
HindIII AAGCTT 3 cut(s) 1277, 2179, 2623
HpaII CCGG 3 cut(s) 846, 1251, 2970
HphI GGTGA 8 cut(s) 374, 644, 1628, 1844, 2618, 2825, 3054, 3145
Hpy188I TCNGA 8 cut(s) 124, 564, 780, 1311, 1558, 2079, 2125, 2664
HpyCH4III ACNGT 5 cut(s) 461, 547, 772, 2864, 3058
HpyCH4IV ACGT 2 cut(s) 589, 2044
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 851, 1095, 1124
HpySE526I ACGT 2 cut(s) 589, 2044
Hsp92I GRCGYC 3 cut(s) 225, 673, 1657
HspAI GCGC 4 cut(s) 394, 1657, 2505, 3073
KasI GGCGCC 1 cut(s) 1656
Kzo9I GATC 6 cut(s) 166, 2328, 2413, 2444, 2554, 3036
LmnI GCTCC 4 cut(s) 296, 2261, 2898, 3056
Lsp1109I GCAGC 3 cut(s) 478, 2026, 2409
LweI GCATC 4 cut(s) 1127, 1255, 2648, 3021
MaeII ACGT 2 cut(s) 589, 2044
MalI GATC 6 cut(s) 168, 2330, 2415, 2446, 2556, 3038
MboI GATC 6 cut(s) 166, 2328, 2413, 2444, 2554, 3036
MfeI CAATTG 1 cut(s) 2004
MflI RGATCY 3 cut(s) 166, 2413, 2444
MhlI GDGCHC 3 cut(s) 188, 1795, 2296
MlsI TGGCCA 1 cut(s) 1761
MluNI TGGCCA 1 cut(s) 1761
Mly113I GGCGCC 1 cut(s) 1657
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 5 cut(s) 197, 467, 542, 572, 1713
Mox20I TGGCCA 1 cut(s) 1761
Mph1103I ATGCAT 2 cut(s) 74, 207
MroXI GAANNNNTTC 3 cut(s) 1855, 1920, 2130
MscI TGGCCA 1 cut(s) 1761
MseI TTAA 6 cut(s) 542, 1001, 1497, 2012, 2235, 2243
MslI CAYNNNNRTG 4 cut(s) 2604, 2846, 3080, 3086
Msp20I TGGCCA 1 cut(s) 1761
MspA1I CMGCKG 1 cut(s) 469
MspI CCGG 3 cut(s) 846, 1251, 2970
MspR9I CCNGG 3 cut(s) 1763, 2897, 2971
MunI CAATTG 1 cut(s) 2004
Mva1269I GAATGC 5 cut(s) 10, 2291, 2829, 3020, 3075
MvaI CCWGG 2 cut(s) 1763, 2897
MwoI GCNNNNNNNGC 4 cut(s) 231, 851, 1095, 1124
NarI GGCGCC 1 cut(s) 1657
NciI CCSGG 1 cut(s) 2971
NcoI CCATGG 1 cut(s) 2847
NdeII GATC 6 cut(s) 166, 2328, 2413, 2444, 2554, 3036
NlaIV GGNNCC 7 cut(s) 292, 580, 853, 1658, 1767, 2312, 2689
NmuCI GTSAC 5 cut(s) 226, 256, 1360, 2606, 3151
NsbI TGCGCA 1 cut(s) 2506
NsiI ATGCAT 2 cut(s) 74, 207
NspI RCATGY 3 cut(s) 234, 415, 1935
NspV TTCGAA 1 cut(s) 2634
PagI TCATGA 2 cut(s) 180, 3081
PceI AGGCCT 1 cut(s) 2988
PciI ACATGT 1 cut(s) 411
PctI GAATGC 5 cut(s) 10, 2291, 2829, 3020, 3075
PdmI GAANNNNTTC 3 cut(s) 1855, 1920, 2130
PflFI GACNNNGTC 3 cut(s) 1522, 2668, 2864
PflMI CCANNNNNTGG 4 cut(s) 110, 1415, 2069, 2714
PfoI TCCNGGA 1 cut(s) 2895
PkrI GCNGC 3 cut(s) 468, 2041, 2424
PleI GAGTC 1 cut(s) 260
PluTI GGCGCC 1 cut(s) 1660
PpsI GAGTC 1 cut(s) 260
PscI ACATGT 1 cut(s) 411
PshBI ATTAAT 1 cut(s) 2012
Psp124BI GAGCTC 3 cut(s) 188, 1795, 2296
Psp6I CCWGG 2 cut(s) 1761, 2895
PspEI GGTNACC 1 cut(s) 1238
PspFI CCCAGC 2 cut(s) 667, 1140
PspGI CCWGG 2 cut(s) 1761, 2895
PspN4I GGNNCC 7 cut(s) 292, 580, 853, 1658, 1767, 2312, 2689
PspPI GGNCC 3 cut(s) 506, 1325, 2072
PstI CTGCAG 1 cut(s) 215
PsuI RGATCY 3 cut(s) 166, 2413, 2444
PsyI GACNNNGTC 3 cut(s) 1522, 2668, 2864
PvuII CAGCTG 1 cut(s) 469
RsaI GTAC 4 cut(s) 297, 463, 1063, 2215
RsaNI GTAC 4 cut(s) 296, 462, 1062, 2214
RseI CAYNNNNRTG 4 cut(s) 2604, 2846, 3080, 3086
SacI GAGCTC 3 cut(s) 188, 1795, 2296
SaqAI TTAA 6 cut(s) 542, 1001, 1497, 2012, 2235, 2243
SatI GCNGC 3 cut(s) 467, 2040, 2423
Sau3AI GATC 6 cut(s) 166, 2328, 2413, 2444, 2554, 3036
Sau96I GGNCC 3 cut(s) 506, 1325, 2072
ScaI AGTACT 1 cut(s) 2215
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 3 cut(s) 1763, 2897, 2971
SduI GDGCHC 3 cut(s) 188, 1795, 2296
SfaNI GCATC 4 cut(s) 1127, 1255, 2648, 3021
SfcI CTRYAG 3 cut(s) 211, 306, 2752
SfoI GGCGCC 1 cut(s) 1658
SfuI TTCGAA 1 cut(s) 2634
SinI GGWCC 1 cut(s) 2072
SmiMI CAYNNNNRTG 4 cut(s) 2604, 2846, 3080, 3086
SmlI CTYRAG 3 cut(s) 1070, 1796, 2344
SmoI CTYRAG 3 cut(s) 1070, 1796, 2344
SpeI ACTAGT 2 cut(s) 1064, 1997
SseBI AGGCCT 1 cut(s) 2988
SsiI CCGC 1 cut(s) 2314
SspDI GGCGCC 1 cut(s) 1656
SspI AATATT 1 cut(s) 2485
SstI GAGCTC 3 cut(s) 188, 1795, 2296
StuI AGGCCT 1 cut(s) 2988
StyD4I CCNGG 3 cut(s) 1761, 2895, 2969
StyI CCWWGG 4 cut(s) 1342, 1633, 2417, 2847
TaaI ACNGT 5 cut(s) 461, 547, 772, 2864, 3058
TaiI ACGT 2 cut(s) 592, 2047
TaqI TCGA 6 cut(s) 737, 1508, 1789, 1958, 2580, 2634
TaqII GACCGA 1 cut(s) 3029
TatI WGTACW 2 cut(s) 1061, 2213
Tru1I TTAA 6 cut(s) 542, 1001, 1497, 2012, 2235, 2243
Tru9I TTAA 6 cut(s) 542, 1001, 1497, 2012, 2235, 2243
TseFI GTSAC 5 cut(s) 226, 256, 1360, 2606, 3151
TseI GCWGC 3 cut(s) 466, 2039, 2422
Tsp45I GTSAC 5 cut(s) 226, 256, 1360, 2606, 3151
TspGWI ACGGA 3 cut(s) 283, 2457, 2816
Tth111I GACNNNGTC 3 cut(s) 1522, 2668, 2864
Van91I CCANNNNNTGG 4 cut(s) 110, 1415, 2069, 2714
VpaK11BI GGWCC 1 cut(s) 2072
VspI ATTAAT 1 cut(s) 2012
XagI CCTNNNNNAGG 2 cut(s) 306, 1845
XbaI TCTAGA 2 cut(s) 85, 2410
XceI RCATGY 3 cut(s) 234, 415, 1935
XmaJI CCTAGG 1 cut(s) 1342
XmiI GTMKAC 1 cut(s) 2193
XmnI GAANNNNTTC 3 cut(s) 1855, 1920, 2130
ZrmI AGTACT 1 cut(s) 2215
Zsp2I ATGCAT 2 cut(s) 74, 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.