Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007807

Physical Location & Seq

Genomic Coordinates Reverse (-)

17766554 .. 17776129

Gene Structure 9576 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1112100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGGGCTCTTGCAAATCCCCTCACATTGTTCTTCTGCTATATCTCTTGCTTTGCTTCTTCTATCATTGTTTTGCCATTGGAGTTGATACGGACCAGACAGCAAACCTGCTTGTCGATGCTTCTCAAGCATCCGCACGCCCAATATCTGACACACTGTTTGGAATTTTCTTTGAGGAGATTAATCACGCTGGTGCTGGCGGGGTGTGGGCTGAGCTTGTAAGCAACAGAGGTTTTGAAGCTGGGGGACCTAACACTCCTTCTAACATTGATCCTTGGGCTATAATTGGGAATGAATCCTCTTTGATTGTGTCAACAGACCGTTCATCATGCTTTGACCGCAATAAGGTTGCACTCCGAATGGAGGTACTATGTGACAGTGAAGGCGCCAATAGCTGCCCTGCTGGGGGTGTTGGAATTTATAACCCTGGGTTCTGGGGCATGAATATAGAAAAGGGGAAGACCTACAGTGTTGTGCTTCATGTTCGTTCATCTGGATCAATCAATGTATCCCTATCACTGACGGGCTCAGATGGGTTGCAGAAACTTGCTGCTGCTAACATTATCGCTTCTGGTTCAGAAGTTTCAAACTGGACAAAATTCCAGATTCAAATGGAAGCCCAAGGAACAAATCCTAATTCAAGACTGCAATTGACAACAAATAGTAAAGGGGTGATATGGTTTGATCAAGTGTCGGCCATTCCCTTGGATACACATAAGGGACATGGATTTCGGAAGGACCTTGTCCAAATGTTGGCTGATTTAAAACCTCGGTTTTTCAGATTTCCAGGTGGCTGTTTCGTTGAAGGTGAATGGTTACGAAATGCATTTCGTTGGAAAGAAACAATTGGACCCTGGGAGGAGAGACCTGGACACTTTGGTGATGTTTGGATGTACTGGACTGATGATGGACTTGGTTATTTTGAGTTTCTCCAACTATCAGAGGACCTTGGCGCATTGCCAATATGGGTGTTCAATAATGGAATCAGTCACAATGATCAAGTTGATACTTCCAATGTCTTACCATTTGTACAGGAAGCTCTTGATGGTATTGAATTTGCTAGAGGCAGTCCTAATTCTACATGGGGTTCTCTTCGTGTTAAAATGGGACACCCAGAACCTTTTGACTTGAGATATGTTGCTATTGGGAATGAGGATTGTAAAAAAACGAACTACCTAGGAAATTACCTTAAGTTCTATAGTGCTATAAGAAAAGCCTATCCTGACATCAAGATGATTTTCTCAAAAATCTATACATCTTCCAGCGACTTGTTTTCTATGGCTAATCATTTTGATCACACATCACGTGATGGTCCCAAGGCTTTTGTGAGCGAGTATGCCGTGACTGGAAAAGATGCCGGAACAGGAAGTCTTCTAGCAGCATTAGCTGAAGCCGGATTTCTTATTGGACTGGAGAAAAACAGTGATGTTGTTGAGATGGCAAGTTATGCACCACTCTTTGTCAATACCCATGACAGGCGGTGGAATCCGGATGCAATTGTCTTTGACTCCTCACAGCTTTACGGGACTCCTAGCTACTGGGTGCAATGTCTTTTCAACGAATCTAGCGGAGCAACTATTTACAACACAACACTCCAAACAAATTCTTCTACTTCACTTCTTGCATCTGCAATTTCGTGGAAAAACTCAAAAAACAAGAATACTTACCTAAGAATAAAGGTTGTGAACTTGGGAACCAACACAGTGAATCTGAAGGTTTCTGTGAATGGGTTGGAGCCAAATTCTATTAGTCCATCTGGATCGACAAAAACTGTGCTTACATCAAACAACTTGATGGATGAGAATTCCTTTAAAGAACCAAAGAAGGTGATACCGAAACAGAGCTTGCTGGAGTCGGCTGGTGAGGAAATGGAAGTTGTAATCTCCCCGCAGTCCTTCACCTCAATTGATTTGTTACTGGAATCTCTCTTTGTTTCTTCTATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009505 GO:0009987 GO:0010383 GO:0010410 GO:0016052 GO:0016787 GO:0016798 GO:0030312 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045493 GO:0046556 GO:0048046 GO:0071554 GO:0071704 GO:0071944 GO:0097599 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Alpha-L-AF_C

Protein Analysis

648

Amino Acids

71.13

Weight (kDa)

5.36

Isoelectric Point (pI)

36.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ASD1_dom	PF22848	251 - 444	1.7e-51	Alpha-L-arabinofuranosidase 1 domain
Alpha-L-AF_C	PF06964	454 - 631	2.2e-25	Alpha-L-arabinofuranosidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007807)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10740 AT3G10740 AT3G10740 AT3G10740
fragaria_vesca	FvH4_1g29140 FvH4_1g29140
malus_domestica	MD12G1112100.v1.1 MD14G1101200.v1.1
prunus_persica	Prupe.7G021200_v2.0.a1 Prupe.7G021200_v2.0.a1 Prupe.7G021200_v2.0.a1
pyrus_communis	pycom12g09150 pycom14g08790
rosa_chinensis	RchiOBHm_Chr3g0494401
rosa_laevigata	RLG00000022674
rosa_multiflora	Rmu_sc0001481.1_g000002
rosa_roxburghii	Rroxscaffold_6G00391120
rosa_rugosa	Rorug03G0265100 Rorug03G0265200
rosa_samantha	Rh3AG315400 Rh3BG350600 Rh3CG347300 Rh3DG349900
rosa_wichuraiana	Rw3G027540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	420
Acc36I	ACCTGC	1 cut(s)	114
AccB1I	GGYRCC	1 cut(s)	383
AccB7I	CCANNNNNTGG	1 cut(s)	751
AccIII	TCCGGA	1 cut(s)	1486
AciI	CCGC	6 cut(s)	132, 198, 337, 1477, 1566, 1886
AclWI	GGATC	3 cut(s)	263, 502, 1765
AcoI	YGGCCR	1 cut(s)	693
AcsI	RAATTY	7 cut(s)	162, 414, 596, 1052, 1600, 1738, 1801
AcuI	CTGAAG	2 cut(s)	1407, 1730
AcvI	CACGTG	1 cut(s)	1304
AcyI	GRCGYC	1 cut(s)	384
AfaI	GTAC	3 cut(s)	366, 893, 1029
AfiI	CCNNNNNNNGG	6 cut(s)	343, 361, 403, 404, 751, 1473
AflII	CTTAAG	1 cut(s)	1187
AgsI	TTSAA	8 cut(s)	236, 585, 608, 639, 803, 973, 1052, 1555
AjnI	CCWGG	4 cut(s)	424, 784, 851, 865
AleI	CACNNNNGTG	2 cut(s)	189, 876
AluBI	AGCT	8 cut(s)	214, 239, 393, 1037, 1385, 1516, 1533, 1842
AluI	AGCT	8 cut(s)	214, 239, 393, 1037, 1385, 1516, 1533, 1842
Alw26I	GTCTC	1 cut(s)	856
AlwI	GGATC	3 cut(s)	263, 502, 1765
Aor13HI	TCCGGA	1 cut(s)	1486
AoxI	GGCC	1 cut(s)	693
ApeKI	GCWGC	4 cut(s)	393, 548, 551, 1376
ApoI	RAATTY	7 cut(s)	162, 414, 596, 1052, 1600, 1738, 1801
ArsI	GACNNNNNNTTYG	4 cut(s)	140, 172, 711, 743
AseI	ATTAAT	1 cut(s)	180
AspA2I	CCTAGG	1 cut(s)	1174
AspLEI	GCGC	2 cut(s)	386, 953
AspS9I	GGNCC	6 cut(s)	91, 245, 736, 848, 943, 1310
AsuHPI	GGTGA	6 cut(s)	682, 818, 890, 1837, 1871, 1888
AvaII	GGWCC	6 cut(s)	91, 245, 736, 848, 943, 1310
AvrII	CCTAGG	1 cut(s)	1174
BanI	GGYRCC	1 cut(s)	383
BanII	GRGCYC	2 cut(s)	8, 527
BbrPI	CACGTG	1 cut(s)	1304
BbsI	GAAGAC	2 cut(s)	464, 1361
BbvI	GCAGC	4 cut(s)	380, 535, 538, 1388
BccI	CCATC	7 cut(s)	524, 899, 1037, 1301, 1429, 1759, 1786
BceAI	ACGGC	1 cut(s)	1322
BciT130I	CCWGG	4 cut(s)	426, 786, 853, 867
BciVI	GTATCC	2 cut(s)	517, 700
BclI	TGATCA	3 cut(s)	682, 994, 1291
BcoDI	GTCTC	1 cut(s)	856
BfaI	CTAG	5 cut(s)	1059, 1175, 1373, 1530, 1563
BfmI	CTRYAG	2 cut(s)	463, 1195
BfoI	RGCGCY	1 cut(s)	387
BfrI	CTTAAG	1 cut(s)	1187
BfuAI	ACCTGC	1 cut(s)	114
BfuI	GTATCC	2 cut(s)	517, 700
BisI	GCNGC	4 cut(s)	394, 549, 552, 1377
BlnI	CCTAGG	1 cut(s)	1174
BlpI	GCTNAGC	1 cut(s)	210
BlsI	GCNGC	4 cut(s)	395, 550, 553, 1378
Bme1390I	CCNGG	4 cut(s)	426, 786, 853, 867
Bme18I	GGWCC	6 cut(s)	91, 245, 736, 848, 943, 1310
BmgT120I	GGNCC	6 cut(s)	91, 245, 736, 848, 943, 1310
BmiI	GGNNCC	6 cut(s)	246, 385, 850, 1312, 1693, 1734
BmrFI	CCNGG	4 cut(s)	426, 786, 853, 867
BmrI	ACTGGG	1 cut(s)	1546
BmsI	GCATC	5 cut(s)	106, 137, 1342, 1480, 1631
BmuI	ACTGGG	1 cut(s)	1546
BpiI	GAAGAC	2 cut(s)	464, 1361
BpmI	CTGGAG	2 cut(s)	1430, 1868
Bpu1102I	GCTNAGC	1 cut(s)	210
BpuEI	CTTGAG	2 cut(s)	108, 1147
BsaAI	YACGTR	1 cut(s)	1304
BsaHI	GRCGYC	1 cut(s)	384
BsaI	GGTCTC	1 cut(s)	856
BsaWI	WCCGGW	1 cut(s)	1486
Bsc4I	CCNNNNNNNGG	6 cut(s)	343, 361, 403, 404, 751, 1473
Bse1I	ACTGG	6 cut(s)	593, 899, 1348, 1413, 1541, 1920
Bse3DI	GCAATG	2 cut(s)	953, 1550
BseAI	TCCGGA	1 cut(s)	1486
BseBI	CCWGG	4 cut(s)	426, 786, 853, 867
BseGI	GGATG	4 cut(s)	128, 894, 1495, 1801
BseLI	CCNNNNNNNGG	6 cut(s)	343, 361, 403, 404, 751, 1473
BseMI	GCAATG	2 cut(s)	953, 1550
BseMII	CTCAG	2 cut(s)	201, 540
BseNI	ACTGG	6 cut(s)	593, 899, 1348, 1413, 1541, 1920
BseRI	GAGGAG	3 cut(s)	188, 872, 1498
BseXI	GCAGC	4 cut(s)	380, 535, 538, 1388
BseYI	CCCAGC	2 cut(s)	239, 401
BshFI	GGCC	1 cut(s)	695
BshNI	GGYRCC	1 cut(s)	383
BsiSI	CCGG	3 cut(s)	1356, 1392, 1487
BslFI	GGGAC	5 cut(s)	258, 732, 1119, 1296, 1537
BslI	CCNNNNNNNGG	6 cut(s)	343, 361, 403, 404, 751, 1473
BsmAI	GTCTC	1 cut(s)	856
BsmFI	GGGAC	5 cut(s)	258, 732, 1119, 1296, 1537
BsnI	GGCC	1 cut(s)	695
Bso31I	GGTCTC	1 cut(s)	856
Bsp1286I	GDGCHC	2 cut(s)	8, 527
Bsp13I	TCCGGA	1 cut(s)	1486
Bsp1407I	TGTACA	1 cut(s)	1027
Bsp143I	GATC	6 cut(s)	268, 494, 682, 994, 1291, 1757
Bsp1720I	GCTNAGC	1 cut(s)	210
BspACI	CCGC	6 cut(s)	132, 198, 337, 1477, 1566, 1886
BspANI	GGCC	1 cut(s)	695
BspCNI	CTCAG	2 cut(s)	202, 539
BspEI	TCCGGA	1 cut(s)	1486
BspLI	GGNNCC	6 cut(s)	246, 385, 850, 1312, 1693, 1734
BspMI	ACCTGC	1 cut(s)	114
BspPI	GGATC	3 cut(s)	263, 502, 1765
BspT107I	GGYRCC	1 cut(s)	383
BspTI	CTTAAG	1 cut(s)	1187
BspTNI	GGTCTC	1 cut(s)	856
BsrDI	GCAATG	2 cut(s)	953, 1550
BsrGI	TGTACA	1 cut(s)	1027
BsrI	ACTGG	6 cut(s)	593, 899, 1348, 1413, 1541, 1920
BssMI	GATC	6 cut(s)	268, 494, 682, 994, 1291, 1757
BssNI	GRCGYC	1 cut(s)	384
BssT1I	CCWWGG	6 cut(s)	272, 619, 702, 946, 1174, 1314
Bst2UI	CCWGG	4 cut(s)	426, 786, 853, 867
Bst4CI	ACNGT	7 cut(s)	156, 320, 377, 467, 1421, 1702, 1771
Bst6I	CTCTTC	1 cut(s)	1095
BstACI	GRCGYC	1 cut(s)	384
BstAFI	CTTAAG	1 cut(s)	1187
BstAPI	GCANNNNNTGC	1 cut(s)	1445
BstAUI	TGTACA	1 cut(s)	1027
BstBAI	YACGTR	1 cut(s)	1304
BstC8I	GCNNGC	3 cut(s)	136, 196, 1844
BstDEI	CTNAG	3 cut(s)	210, 526, 1667
BstF5I	GGATG	4 cut(s)	128, 894, 1495, 1801
BstH2I	RGCGCY	1 cut(s)	387
BstHHI	GCGC	2 cut(s)	386, 953
BstKTI	GATC	6 cut(s)	271, 497, 685, 997, 1294, 1760
BstMAI	GTCTC	1 cut(s)	856
BstMBI	GATC	6 cut(s)	268, 494, 682, 994, 1291, 1757
BstMWI	GCNNNNNNNGC	5 cut(s)	125, 336, 390, 1382, 1445
BstNI	CCWGG	4 cut(s)	426, 786, 853, 867
BstSCI	CCNGG	4 cut(s)	424, 784, 851, 865
BstSFI	CTRYAG	2 cut(s)	463, 1195
BstV1I	GCAGC	4 cut(s)	380, 535, 538, 1388
BstV2I	GAAGAC	2 cut(s)	464, 1361
BstXI	CCANNNNNNTGG	1 cut(s)	703
BsuI	GTATCC	2 cut(s)	517, 700
BsuRI	GGCC	1 cut(s)	695
BtsCI	GGATG	4 cut(s)	128, 894, 1495, 1801
BtsIMutI	CAGTG	6 cut(s)	152, 382, 472, 515, 1426, 1707
BveI	ACCTGC	1 cut(s)	114
Cac8I	GCNNGC	3 cut(s)	136, 196, 1844
CfoI	GCGC	2 cut(s)	386, 953
Cfr13I	GGNCC	6 cut(s)	91, 245, 736, 848, 943, 1310
Csp6I	GTAC	3 cut(s)	365, 892, 1028
CviAII	CATG	6 cut(s)	327, 439, 479, 722, 1080, 1469
CviQI	GTAC	3 cut(s)	365, 892, 1028
DdeI	CTNAG	3 cut(s)	210, 526, 1667
DinI	GGCGCC	1 cut(s)	385
DpnI	GATC	6 cut(s)	270, 496, 684, 996, 1293, 1759
DpnII	GATC	6 cut(s)	268, 494, 682, 994, 1291, 1757
DraI	TTTAAA	2 cut(s)	762, 1810
EaeI	YGGCCR	1 cut(s)	693
Eam1104I	CTCTTC	1 cut(s)	1095
EarI	CTCTTC	1 cut(s)	1095
Eco130I	CCWWGG	6 cut(s)	272, 619, 702, 946, 1174, 1314
Eco24I	GRGCYC	2 cut(s)	8, 527
Eco31I	GGTCTC	1 cut(s)	856
Eco47I	GGWCC	6 cut(s)	91, 245, 736, 848, 943, 1310
Eco57I	CTGAAG	2 cut(s)	1407, 1730
Eco72I	CACGTG	1 cut(s)	1304
EcoO109I	RGGNCCY	3 cut(s)	245, 736, 943
EcoRI	GAATTC	1 cut(s)	1801
EcoRII	CCWGG	4 cut(s)	424, 784, 851, 865
EcoT14I	CCWWGG	6 cut(s)	272, 619, 702, 946, 1174, 1314
EcoT22I	ATGCAT	1 cut(s)	826
EcoT38I	GRGCYC	2 cut(s)	8, 527
EgeI	GGCGCC	1 cut(s)	385
EheI	GGCGCC	1 cut(s)	385
ErhI	CCWWGG	6 cut(s)	272, 619, 702, 946, 1174, 1314
FaeI	CATG	6 cut(s)	330, 442, 482, 725, 1083, 1472
FaqI	GGGAC	5 cut(s)	258, 732, 1119, 1296, 1537
FatI	CATG	6 cut(s)	326, 438, 478, 721, 1079, 1468
FauI	CCCGC	2 cut(s)	191, 1893
FbaI	TGATCA	3 cut(s)	682, 994, 1291
Fnu4HI	GCNGC	4 cut(s)	394, 549, 552, 1377
FokI	GGATG	4 cut(s)	115, 901, 1502, 1808
FriOI	GRGCYC	2 cut(s)	8, 527
Fsp4HI	GCNGC	4 cut(s)	394, 549, 552, 1377
FspBI	CTAG	5 cut(s)	1059, 1175, 1373, 1530, 1563
GlaI	GCGC	2 cut(s)	385, 952
GluI	GCNGC	4 cut(s)	394, 549, 552, 1377
GsaI	CCCAGC	2 cut(s)	243, 405
GsuI	CTGGAG	2 cut(s)	1430, 1868
HaeII	RGCGCY	1 cut(s)	387
HaeIII	GGCC	1 cut(s)	695
HapII	CCGG	3 cut(s)	1356, 1392, 1487
HhaI	GCGC	2 cut(s)	386, 953
Hin1I	GRCGYC	1 cut(s)	384
Hin1II	CATG	6 cut(s)	330, 442, 482, 725, 1083, 1472
Hin6I	GCGC	2 cut(s)	384, 951
HinP1I	GCGC	2 cut(s)	384, 951
HincII	GTYRAC	1 cut(s)	312
HindII	GTYRAC	1 cut(s)	312
HpaII	CCGG	3 cut(s)	1356, 1392, 1487
HphI	GGTGA	6 cut(s)	682, 818, 890, 1837, 1871, 1888
Hpy166II	GTNNAC	2 cut(s)	312, 1684
Hpy188I	TCNGA	8 cut(s)	148, 356, 529, 577, 732, 779, 940, 1710
Hpy188III	TCNNGA	8 cut(s)	492, 601, 639, 1040, 1220, 1228, 1487, 1755
Hpy8I	GTNNAC	2 cut(s)	312, 1684
HpyAV	CCTTC	7 cut(s)	267, 374, 727, 797, 1705, 1816, 1903
HpyCH4III	ACNGT	7 cut(s)	156, 320, 377, 467, 1421, 1702, 1771
HpyCH4IV	ACGT	1 cut(s)	1303
HpyF10VI	GCNNNNNNNGC	5 cut(s)	125, 336, 390, 1382, 1445
HpyF3I	CTNAG	3 cut(s)	210, 526, 1667
HpySE526I	ACGT	1 cut(s)	1303
Hsp92I	GRCGYC	1 cut(s)	384
Hsp92II	CATG	6 cut(s)	330, 442, 482, 725, 1083, 1472
HspAI	GCGC	2 cut(s)	384, 951
KasI	GGCGCC	1 cut(s)	383
Kpn2I	TCCGGA	1 cut(s)	1486
Ksp22I	TGATCA	3 cut(s)	682, 994, 1291
Kzo9I	GATC	6 cut(s)	268, 494, 682, 994, 1291, 1757
LmnI	GCTCC	2 cut(s)	1568, 1732
Lsp1109I	GCAGC	4 cut(s)	380, 535, 538, 1388
LweI	GCATC	5 cut(s)	106, 137, 1342, 1480, 1631
MaeI	CTAG	5 cut(s)	1059, 1175, 1373, 1530, 1563
MaeII	ACGT	1 cut(s)	1303
MaeIII	GTNAC	5 cut(s)	371, 813, 986, 1339, 1911
MalI	GATC	6 cut(s)	270, 496, 684, 996, 1293, 1759
MboI	GATC	6 cut(s)	268, 494, 682, 994, 1291, 1757
MboII	GAAGA	8 cut(s)	23, 49, 469, 1082, 1248, 1361, 1596, 1926
MfeI	CAATTG	4 cut(s)	647, 843, 1494, 1902
MhlI	GDGCHC	2 cut(s)	8, 527
Mly113I	GGCGCC	1 cut(s)	384
MlyI	GAGTC	3 cut(s)	1499, 1519, 1859
MmeI	TCCRAC	4 cut(s)	391, 812, 955, 1710
Mph1103I	ATGCAT	1 cut(s)	826
MroI	TCCGGA	1 cut(s)	1486
MseI	TTAA	5 cut(s)	180, 761, 1098, 1188, 1809
MslI	CAYNNNNRTG	3 cut(s)	189, 876, 1229
MspCI	CTTAAG	1 cut(s)	1187
MspI	CCGG	3 cut(s)	1356, 1392, 1487
MspR9I	CCNGG	4 cut(s)	426, 786, 853, 867
MunI	CAATTG	4 cut(s)	647, 843, 1494, 1902
MvaI	CCWGG	4 cut(s)	426, 786, 853, 867
MwoI	GCNNNNNNNGC	5 cut(s)	125, 336, 390, 1382, 1445
NarI	GGCGCC	1 cut(s)	384
NdeII	GATC	6 cut(s)	268, 494, 682, 994, 1291, 1757
NlaIII	CATG	6 cut(s)	330, 442, 482, 725, 1083, 1472
NlaIV	GGNNCC	6 cut(s)	246, 385, 850, 1312, 1693, 1734
NmuCI	GTSAC	3 cut(s)	371, 986, 1339
NsiI	ATGCAT	1 cut(s)	826
OliI	CACNNNNGTG	2 cut(s)	189, 876
PasI	CCCWGGG	2 cut(s)	425, 852
PfeI	GAWTC	7 cut(s)	293, 604, 981, 1483, 1559, 1705, 1919
PflFI	GACNNNGTC	1 cut(s)	740
PflMI	CCANNNNNTGG	1 cut(s)	751
PkrI	GCNGC	4 cut(s)	395, 550, 553, 1378
PleI	GAGTC	3 cut(s)	1499, 1519, 1858
PluTI	GGCGCC	1 cut(s)	387
PmaCI	CACGTG	1 cut(s)	1304
PmlI	CACGTG	1 cut(s)	1304
PpsI	GAGTC	3 cut(s)	1499, 1519, 1858
Ppu21I	YACGTR	1 cut(s)	1304
PpuMI	RGGWCCY	3 cut(s)	245, 736, 943
PshBI	ATTAAT	1 cut(s)	180
PsiI	TTATAA	1 cut(s)	420
Psp5II	RGGWCCY	3 cut(s)	245, 736, 943
Psp6I	CCWGG	4 cut(s)	424, 784, 851, 865
PspCI	CACGTG	1 cut(s)	1304
PspFI	CCCAGC	2 cut(s)	239, 401
PspGI	CCWGG	4 cut(s)	424, 784, 851, 865
PspN4I	GGNNCC	6 cut(s)	246, 385, 850, 1312, 1693, 1734
PspPI	GGNCC	6 cut(s)	91, 245, 736, 848, 943, 1310
PspPPI	RGGWCCY	3 cut(s)	245, 736, 943
PsyI	GACNNNGTC	1 cut(s)	740
RsaI	GTAC	3 cut(s)	366, 893, 1029
RsaNI	GTAC	3 cut(s)	365, 892, 1028
RseI	CAYNNNNRTG	3 cut(s)	189, 876, 1229
SaqAI	TTAA	5 cut(s)	180, 761, 1098, 1188, 1809
SatI	GCNGC	4 cut(s)	394, 549, 552, 1377
Sau3AI	GATC	6 cut(s)	268, 494, 682, 994, 1291, 1757
Sau96I	GGNCC	6 cut(s)	91, 245, 736, 848, 943, 1310
SchI	GAGTC	3 cut(s)	1499, 1519, 1859
ScrFI	CCNGG	4 cut(s)	426, 786, 853, 867
SduI	GDGCHC	2 cut(s)	8, 527
SfaNI	GCATC	5 cut(s)	106, 137, 1342, 1480, 1631
SfcI	CTRYAG	2 cut(s)	463, 1195
SfoI	GGCGCC	1 cut(s)	385
SinI	GGWCC	6 cut(s)	91, 245, 736, 848, 943, 1310
SmiMI	CAYNNNNRTG	3 cut(s)	189, 876, 1229
SmlI	CTYRAG	3 cut(s)	123, 1126, 1187
SmoI	CTYRAG	3 cut(s)	123, 1126, 1187
SsiI	CCGC	6 cut(s)	132, 198, 337, 1477, 1566, 1886
SspDI	GGCGCC	1 cut(s)	383
SspMI	CTAG	5 cut(s)	1059, 1175, 1373, 1530, 1563
StyD4I	CCNGG	4 cut(s)	424, 784, 851, 865
StyI	CCWWGG	6 cut(s)	272, 619, 702, 946, 1174, 1314
TaaI	ACNGT	7 cut(s)	156, 320, 377, 467, 1421, 1702, 1771
TaiI	ACGT	1 cut(s)	1306
TaqI	TCGA	2 cut(s)	114, 1760
TatI	WGTACW	2 cut(s)	891, 1027
TfiI	GAWTC	7 cut(s)	293, 604, 981, 1483, 1559, 1705, 1919
Tru1I	TTAA	5 cut(s)	180, 761, 1098, 1188, 1809
Tru9I	TTAA	5 cut(s)	180, 761, 1098, 1188, 1809
TscAI	CASTG	6 cut(s)	159, 382, 472, 522, 1426, 1707
TseFI	GTSAC	3 cut(s)	371, 986, 1339
TseI	GCWGC	4 cut(s)	393, 548, 551, 1376
Tsp45I	GTSAC	3 cut(s)	371, 986, 1339
TspDTI	ATGAA	5 cut(s)	306, 312, 455, 467, 477
TspGWI	ACGGA	1 cut(s)	104
TspRI	CASTG	6 cut(s)	159, 382, 472, 522, 1426, 1707
Tth111I	GACNNNGTC	1 cut(s)	740
Van91I	CCANNNNNTGG	1 cut(s)	751
Vha464I	CTTAAG	1 cut(s)	1187
VpaK11BI	GGWCC	6 cut(s)	91, 245, 736, 848, 943, 1310
VspI	ATTAAT	1 cut(s)	180
XapI	RAATTY	7 cut(s)	162, 414, 596, 1052, 1600, 1738, 1801
XmaJI	CCTAGG	1 cut(s)	1174
XspI	CTAG	5 cut(s)	1059, 1175, 1373, 1530, 1563
Zsp2I	ATGCAT	1 cut(s)	826

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.