Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0007807

Physical Location & Seq

Genomic Coordinates Forward (+)

7415838 .. 7431202

Gene Structure 15365 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00391120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGAAGGCCGTCGTCTTGCTCTGCTGCTGCTTTCTGTTCCAATGTTTCGCTGTTGGAGCTGATACGAACCAGACAGCAGAGCTGCTTGTTGATGCTTCTCAATCATCCGGACGACCAATATCTCCAACACTCTTTGGAATTTTCTTCGAGGAGATTAACCATGCTGGCGCTGGTGGGTTGTGGGCTGAACTTGTAAGCAACAGAGGTTTTGAATCTGGGGGACCTAACACTCCTTCCAACATTGATCCCTGGTCAATTATTGGGAATGAGTCATCTTTGCTTGTATCGACGGACCGGTCGTCATGTTTCGAGCGCAATAAAGTTGCACTTCGAATGGAGGTTCTATGTGACAGCCAAGATGCCAATATCTGTCCTTCTGGGGGTGTTGGTATTTATAACCCTGGGTTCTGGGGCATGAATGTCGAAAAAGGGAAGACCTACATTGTAACTCTTTATGTTCGTTCATCTGGAGCAATCAATGTATCTGTATCGTTGACGAGTTCCAATGGATTGCAGACTCTTGCTGCTGATAACATTATAGCTTCTGTTTCAGAAGTTTCAAACTGGACGAAATTTGAAGTTCTGTTGGAAGCCAAAGCAACAAATCCTAGTTCAAGATTGCAATTGACAACCACCAGAAAAGGGTTGATATGGTTTGATCAAGTGTCAGTCATGCCTTCGGACACATACAAGGGACATGGATTTAGGAAGGACCTTGTCGACATGTTGGCAGATCTAAAACCCCAATTTATTAGATTTCCAGGTGGCTGTTTTGTTGAAGGTGAATGGTTAAGAAATGCATTTCGTTGGAAAGAAACAATTGGACCATGGGAAGAGAGACCTGGACACTTTGGTGATGTTTGGATGTACTGGACTGACGATGGACTTGGTTATTTTGAGTTTTTGCAACTAGCAGAGGACCTTGGTACACGGCCAATATGGGTGTTCAACAATGGAATCAGCCACAATGATCAAGTTGATACTTCCAATGTCTTACCGTTTGTACAGGAAGCTCTTGATGGTATTGAGTTTGCTAGGGGCGATCCTGATTCTACATGGGGTTCTCTCCGTGCCGCAATGGGACACCCAGAGCCCTTTGACTTGAGATATGTTGCTGTTGGGAATGAGGATTGTGGCAAAAAGAATTACCGAGGAAATTACCTTAAGTTCTACAGTGCTATAAAGCGTGCCTATCCGGACATCCAAATAATTTCTAACTGCGATGGCTCTTCTCAACCTTTGGATCATCCAGCTGATTTGTATGACTTTCATGTTTATACTTCAGCAAGCAACATGTTTTCGATGGCTCATCGGTTTGATCGTGCACCACGTAAGGGTCCCAAGGCTTTTGTGAGTGAGTATGCTGTGACCGGAAAAGATGCTGGAGCAGGAAGTCTTCTCGCAGCATTGGCAGAAGCCGGATTTCTGATTGGGCTTGAAAAAAACAGTGATGTTGTTGAGATGGCAAGTTATGCACCACTCTTTGTGAATGCCAATAACAAGCGGTGGAACCCAGATGCAATCGTGTTTAACTCCTCACAGCTGTATGGAACTCCTAGCTACTGGGTGCAATGTTTTTTTAGTGAATCAAGTGGAGCAACTATTTTTAATGCAACACTCCAAACAAATTCTTCTACTTCACTTCTTGCATCTGCAATTTCGTGGAAAAGTTCTGAAGATCAGAATACTTACCTAAGAATTAAGGTAACTGTTCTATGGAAACAATTTTCGACTGCTGGGAGTGACAACTTCTTGTTCTTGAGGGGAGTTGCTGAATTGCATTGGTTAGTTCAGATCATGGGTGGTTTGCCTTATGGTGGATCCGATGAGGAAGGTGACAGCTCTGTAATTGAGGGCCTGGAAGTTGGTTCTCAGCCACTCGTGTCTTTGGCTTGCATATGGTCGATCAGGGATGCTGACGGGCCTGTCCACCTGAGTTGGAGGGCCAGGATTGTGAACTTTGGAAGCAACATAGTAAATCTTACGATCCGTGTTGATGGGTTGGGGCCAAATTTTGTTGGTCTGTCTGGATCAACCAAGACTGTGCTCACATCTAAAAATGTGATGGATGAGAACTCGTTTGGTGAGCCAAAGAAGGTCATACCGAACCTGAGTCTACTTGAACAAGCTGGTGAGGAAATGGATGTTGGACTTTCTGCACATTCCTTCACTTCAATTGATTTGTTAATGGAACCAAGTTATATCAGGACTGGAGCTGATTCTTTCTCCCTGTCTTCTATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009505 GO:0009987 GO:0010383 GO:0010410 GO:0016052 GO:0016787 GO:0016798 GO:0030312 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045493 GO:0046556 GO:0048046 GO:0071554 GO:0071704 GO:0071944 GO:0097599 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Alpha-L-AF_C

Protein Analysis

747

Amino Acids

81.8

Weight (kDa)

4.9

Isoelectric Point (pI)

33.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ASD1_dom	PF22848	243 - 444	4.3e-59	Alpha-L-arabinofuranosidase 1 domain
Alpha-L-AF_C	PF06964	464 - 722	1.5e-21	Alpha-L-arabinofuranosidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007807)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10740 AT3G10740 AT3G10740 AT3G10740
fragaria_vesca	FvH4_1g29140 FvH4_1g29140
malus_domestica	MD12G1112100.v1.1 MD14G1101200.v1.1
prunus_persica	Prupe.7G021200_v2.0.a1 Prupe.7G021200_v2.0.a1 Prupe.7G021200_v2.0.a1
pyrus_communis	pycom12g09150 pycom14g08790
rosa_chinensis	RchiOBHm_Chr3g0494401
rosa_laevigata	RLG00000022674
rosa_multiflora	Rmu_sc0001481.1_g000002
rosa_roxburghii	Rroxscaffold_6G00391120
rosa_rugosa	Rorug03G0265100 Rorug03G0265200
rosa_samantha	Rh3AG315400 Rh3BG350600 Rh3CG347300 Rh3DG349900
rosa_wichuraiana	Rw3G027540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	396
AasI	GACNNNNNNGTC	2 cut(s)	295, 1925
AccI	GTMKAC	2 cut(s)	720, 2116
AccIII	TCCGGA	2 cut(s)	107, 1195
AciI	CCGC	2 cut(s)	1074, 1504
AclWI	GGATC	7 cut(s)	239, 1037, 1251, 1815, 1828, 1981, 2038
AcoI	YGGCCR	1 cut(s)	932
AcsI	RAATTY	4 cut(s)	138, 572, 1627, 2011
AcuI	CTGAAG	2 cut(s)	1266, 1695
AfaI	GTAC	3 cut(s)	869, 928, 1005
AfiI	CCNNNNNNNGG	4 cut(s)	380, 642, 1334, 1817
AflII	CTTAAG	1 cut(s)	1163
AflIII	ACRYGT	2 cut(s)	723, 1293
AgeI	ACCGGT	1 cut(s)	294
AgsI	TTSAA	9 cut(s)	212, 561, 578, 615, 779, 949, 1439, 2123, 2175
AhdI	GACNNNNNGTC	1 cut(s)	298
AjnI	CCWGG	6 cut(s)	248, 400, 760, 841, 1857, 1946
AjuI	GAANNNNNNNTTGG	2 cut(s)	2130, 2162
AleI	CACNNNNGTG	1 cut(s)	852
Alw21I	GWGCWC	2 cut(s)	1327, 2049
Alw26I	GTCTC	1 cut(s)	832
Alw44I	GTGCAC	1 cut(s)	1323
AlwI	GGATC	7 cut(s)	239, 1037, 1251, 1815, 1828, 1981, 2038
Aor13HI	TCCGGA	2 cut(s)	107, 1195
AoxI	GGCC	6 cut(s)	6, 932, 1855, 1922, 1944, 2006
ApaLI	GTGCAC	1 cut(s)	1323
ApeKI	GCWGC	5 cut(s)	24, 27, 82, 524, 1403
ApoI	RAATTY	4 cut(s)	138, 572, 1627, 2011
AsiGI	ACCGGT	1 cut(s)	294
AspLEI	GCGC	2 cut(s)	170, 315
AsuHPI	GGTGA	5 cut(s)	794, 866, 1847, 2096, 2144
AsuII	TTCGAA	1 cut(s)	331
AvaII	GGWCC	6 cut(s)	221, 292, 712, 824, 919, 1337
BaeGI	GKGCMC	1 cut(s)	1327
BamHI	GGATCC	1 cut(s)	1820
BanII	GRGCYC	1 cut(s)	1095
BauI	CACGAG	1 cut(s)	1880
BbsI	GAAGAC	3 cut(s)	440, 1388, 2226
Bbv12I	GWGCWC	2 cut(s)	1327, 2049
BbvI	GCAGC	5 cut(s)	11, 14, 69, 511, 1415
BccI	CCATC	7 cut(s)	875, 1013, 1217, 1297, 1456, 1991, 2059
BceAI	ACGGC	1 cut(s)	947
BcgI	CGANNNNNNTGC	2 cut(s)	403, 437
BciT130I	CCWGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
BclI	TGATCA	2 cut(s)	658, 970
BcoDI	GTCTC	1 cut(s)	832
BfaI	CTAG	4 cut(s)	609, 911, 1035, 1557
BfmI	CTRYAG	1 cut(s)	1171
BfoI	RGCGCY	1 cut(s)	171
BfrI	CTTAAG	1 cut(s)	1163
BglII	AGATCT	1 cut(s)	733
BisI	GCNGC	6 cut(s)	25, 28, 83, 525, 1074, 1404
BlsI	GCNGC	6 cut(s)	26, 29, 84, 526, 1075, 1405
Bme1390I	CCNGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
Bme18I	GGWCC	6 cut(s)	221, 292, 712, 824, 919, 1337
BmeRI	GACNNNNNGTC	1 cut(s)	298
BmiI	GGNNCC	7 cut(s)	222, 1338, 1339, 1511, 1822, 2007, 2193
BmrFI	CCNGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
BmrI	ACTGGG	1 cut(s)	1573
BmsI	GCATC	6 cut(s)	82, 349, 1369, 1507, 1658, 1903
BmuI	ACTGGG	1 cut(s)	1573
BpiI	GAAGAC	3 cut(s)	440, 1388, 2226
BpmI	CTGGAG	3 cut(s)	489, 1404, 2232
Bpu14I	TTCGAA	1 cut(s)	331
BpuEI	CTTGAG	2 cut(s)	1123, 1780
BsaAI	YACGTR	1 cut(s)	1331
BsaI	GGTCTC	1 cut(s)	832
BsaJI	CCNNGG	7 cut(s)	248, 400, 401, 827, 922, 1150, 1341
BsaWI	WCCGGW	4 cut(s)	107, 294, 1195, 1370
Bsc4I	CCNNNNNNNGG	4 cut(s)	380, 642, 1334, 1817
Bse118I	RCCGGY	1 cut(s)	294
Bse1I	ACTGG	4 cut(s)	569, 875, 1568, 2215
Bse3DI	GCAATG	2 cut(s)	1083, 1577
BseAI	TCCGGA	2 cut(s)	107, 1195
BseBI	CCWGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
BseDI	CCNNGG	7 cut(s)	248, 400, 401, 827, 922, 1150, 1341
BseGI	GGATG	7 cut(s)	104, 870, 1200, 1246, 1918, 2074, 2149
BseLI	CCNNNNNNNGG	4 cut(s)	380, 642, 1334, 1817
BseMI	GCAATG	2 cut(s)	1083, 1577
BseMII	CTCAG	3 cut(s)	1886, 1925, 2102
BseNI	ACTGG	4 cut(s)	569, 875, 1568, 2215
BseRI	GAGGAG	2 cut(s)	164, 1525
BseSI	GKGCMC	1 cut(s)	1327
BseXI	GCAGC	5 cut(s)	11, 14, 69, 511, 1415
BseYI	CCCAGC	1 cut(s)	1736
BsgI	GTGCAG	1 cut(s)	2142
Bsh1285I	CGRYCG	1 cut(s)	299
BshFI	GGCC	6 cut(s)	8, 934, 1857, 1924, 1946, 2008
BshTI	ACCGGT	1 cut(s)	294
BsiEI	CGRYCG	1 cut(s)	299
BsiHKAI	GWGCWC	2 cut(s)	1327, 2049
BsiSI	CCGG	5 cut(s)	108, 295, 1196, 1371, 1419
BslFI	GGGAC	4 cut(s)	234, 708, 1095, 1323
BslI	CCNNNNNNNGG	4 cut(s)	380, 642, 1334, 1817
BsmAI	GTCTC	1 cut(s)	832
BsmFI	GGGAC	4 cut(s)	234, 708, 1095, 1323
BsmI	GAATGC	1 cut(s)	1495
BsnI	GGCC	6 cut(s)	8, 934, 1857, 1924, 1946, 2008
Bso31I	GGTCTC	1 cut(s)	832
Bsp119I	TTCGAA	1 cut(s)	331
Bsp1286I	GDGCHC	3 cut(s)	1095, 1327, 2049
Bsp13I	TCCGGA	2 cut(s)	107, 1195
Bsp1407I	TGTACA	1 cut(s)	1003
Bsp19I	CCATGG	1 cut(s)	827
BspACI	CCGC	2 cut(s)	1074, 1504
BspANI	GGCC	6 cut(s)	8, 934, 1857, 1924, 1946, 2008
BspCNI	CTCAG	3 cut(s)	1885, 1926, 2103
BspEI	TCCGGA	2 cut(s)	107, 1195
BspLI	GGNNCC	7 cut(s)	222, 1338, 1339, 1511, 1822, 2007, 2193
BspPI	GGATC	7 cut(s)	239, 1037, 1251, 1815, 1828, 1981, 2038
BspQI	GCTCTTC	1 cut(s)	1234
BspT104I	TTCGAA	1 cut(s)	331
BspTI	CTTAAG	1 cut(s)	1163
BspTNI	GGTCTC	1 cut(s)	832
BsrDI	GCAATG	2 cut(s)	1083, 1577
BsrFI	RCCGGY	1 cut(s)	294
BsrGI	TGTACA	1 cut(s)	1003
BsrI	ACTGG	4 cut(s)	569, 875, 1568, 2215
BssAI	RCCGGY	1 cut(s)	294
BssECI	CCNNGG	7 cut(s)	248, 400, 401, 827, 922, 1150, 1341
BssSI	CACGAG	1 cut(s)	1880
BssT1I	CCWWGG	3 cut(s)	827, 922, 1341
Bst2BI	CACGAG	1 cut(s)	1880
Bst2UI	CCWGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
Bst4CI	ACNGT	5 cut(s)	999, 1175, 1448, 1711, 2044
Bst6I	CTCTTC	2 cut(s)	828, 1234
BstAFI	CTTAAG	1 cut(s)	1163
BstAPI	GCANNNNNTGC	1 cut(s)	1472
BstAUI	TGTACA	1 cut(s)	1003
BstBAI	YACGTR	1 cut(s)	1331
BstBI	TTCGAA	1 cut(s)	331
BstC8I	GCNNGC	4 cut(s)	166, 1188, 1288, 1894
BstDEI	CTNAG	4 cut(s)	1694, 1872, 1934, 2111
BstDSI	CCRYGG	1 cut(s)	827
BstF5I	GGATG	7 cut(s)	104, 870, 1200, 1246, 1918, 2074, 2149
BstH2I	RGCGCY	1 cut(s)	171
BstHHI	GCGC	2 cut(s)	170, 315
BstMAI	GTCTC	1 cut(s)	832
BstMCI	CGRYCG	1 cut(s)	299
BstMWI	GCNNNNNNNGC	3 cut(s)	56, 1409, 1472
BstNI	CCWGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
BstNSI	RCATGY	2 cut(s)	727, 1297
BstSCI	CCNGG	6 cut(s)	248, 400, 760, 841, 1857, 1946
BstSFI	CTRYAG	1 cut(s)	1171
BstSLI	GKGCMC	1 cut(s)	1327
BstV1I	GCAGC	5 cut(s)	11, 14, 69, 511, 1415
BstV2I	GAAGAC	3 cut(s)	440, 1388, 2226
BstX2I	RGATCY	2 cut(s)	733, 1820
BstYI	RGATCY	2 cut(s)	733, 1820
BsuRI	GGCC	6 cut(s)	8, 934, 1857, 1924, 1946, 2008
BtgI	CCRYGG	1 cut(s)	827
BtgZI	GCGATG	1 cut(s)	1236
BtsCI	GGATG	7 cut(s)	104, 870, 1200, 1246, 1918, 2074, 2149
BtsIMutI	CAGTG	2 cut(s)	1180, 1453
Cac8I	GCNNGC	4 cut(s)	166, 1188, 1288, 1894
CfoI	GCGC	2 cut(s)	170, 315
Cfr10I	RCCGGY	1 cut(s)	294
CpoI	CGGWCCG	1 cut(s)	292
Csp6I	GTAC	3 cut(s)	868, 927, 1004
CspAI	ACCGGT	1 cut(s)	294
CspCI	CAANNNNNGTGG	2 cut(s)	1919, 1954
CspI	CGGWCCG	1 cut(s)	292
CviQI	GTAC	3 cut(s)	868, 927, 1004
DdeI	CTNAG	4 cut(s)	1694, 1872, 1934, 2111
DrdI	GACNNNNNNGTC	2 cut(s)	295, 1925
DriI	GACNNNNNGTC	1 cut(s)	298
DseDI	GACNNNNNNGTC	2 cut(s)	295, 1925
EaeI	YGGCCR	1 cut(s)	932
Eam1104I	CTCTTC	2 cut(s)	828, 1234
Eam1105I	GACNNNNNGTC	1 cut(s)	298
EarI	CTCTTC	2 cut(s)	828, 1234
Eco130I	CCWWGG	3 cut(s)	827, 922, 1341
Eco24I	GRGCYC	1 cut(s)	1095
Eco31I	GGTCTC	1 cut(s)	832
Eco47I	GGWCC	6 cut(s)	221, 292, 712, 824, 919, 1337
Eco57I	CTGAAG	2 cut(s)	1266, 1695
EcoO109I	RGGNCCY	5 cut(s)	221, 712, 919, 1337, 1855
EcoRII	CCWGG	6 cut(s)	248, 400, 760, 841, 1857, 1946
EcoT14I	CCWWGG	3 cut(s)	827, 922, 1341
EcoT22I	ATGCAT	1 cut(s)	802
EcoT38I	GRGCYC	1 cut(s)	1095
ErhI	CCWWGG	3 cut(s)	827, 922, 1341
FalI	AAGNNNNNCTT	2 cut(s)	312, 344
FaqI	GGGAC	4 cut(s)	234, 708, 1095, 1323
FauNDI	CATATG	1 cut(s)	1898
FbaI	TGATCA	2 cut(s)	658, 970
FblI	GTMKAC	2 cut(s)	720, 2116
Fnu4HI	GCNGC	6 cut(s)	25, 28, 83, 525, 1074, 1404
FokI	GGATG	7 cut(s)	91, 877, 1187, 1233, 1925, 2081, 2156
FriOI	GRGCYC	1 cut(s)	1095
Fsp4HI	GCNGC	6 cut(s)	25, 28, 83, 525, 1074, 1404
FspBI	CTAG	4 cut(s)	609, 911, 1035, 1557
GlaI	GCGC	2 cut(s)	169, 314
GluI	GCNGC	6 cut(s)	25, 28, 83, 525, 1074, 1404
GsaI	CCCAGC	1 cut(s)	1740
GsuI	CTGGAG	3 cut(s)	489, 1404, 2232
HaeII	RGCGCY	1 cut(s)	171
HaeIII	GGCC	6 cut(s)	8, 934, 1857, 1924, 1946, 2008
HapII	CCGG	5 cut(s)	108, 295, 1196, 1371, 1419
HhaI	GCGC	2 cut(s)	170, 315
Hin6I	GCGC	2 cut(s)	168, 313
HinP1I	GCGC	2 cut(s)	168, 313
HincII	GTYRAC	2 cut(s)	495, 721
HindII	GTYRAC	2 cut(s)	495, 721
HinfI	GANTC	8 cut(s)	212, 269, 517, 957, 1049, 1586, 2113, 2219
HpaII	CCGG	5 cut(s)	108, 295, 1196, 1371, 1419
HphI	GGTGA	5 cut(s)	794, 866, 1847, 2096, 2144
Hpy166II	GTNNAC	7 cut(s)	495, 721, 929, 1325, 1930, 1957, 2117
Hpy188I	TCNGA	7 cut(s)	553, 682, 1428, 1675, 1683, 1794, 1825
Hpy188III	TCNNGA	9 cut(s)	108, 468, 615, 1016, 1046, 1196, 1759, 2028, 2206
Hpy8I	GTNNAC	7 cut(s)	495, 721, 929, 1325, 1930, 1957, 2117
Hpy99I	CGWCG	2 cut(s)	14, 292
HpyAV	CCTTC	8 cut(s)	243, 384, 687, 703, 773, 1826, 2089, 2176
HpyCH4III	ACNGT	5 cut(s)	999, 1175, 1448, 1711, 2044
HpyCH4IV	ACGT	1 cut(s)	1330
HpyF10VI	GCNNNNNNNGC	3 cut(s)	56, 1409, 1472
HpyF3I	CTNAG	4 cut(s)	1694, 1872, 1934, 2111
HpySE526I	ACGT	1 cut(s)	1330
HspAI	GCGC	2 cut(s)	168, 313
KflI	GGGWCCC	1 cut(s)	1337
Kpn2I	TCCGGA	2 cut(s)	107, 1195
Ksp22I	TGATCA	2 cut(s)	658, 970
LguI	GCTCTTC	1 cut(s)	1234
LmnI	GCTCC	5 cut(s)	56, 470, 1385, 1595, 2213
Lsp1109I	GCAGC	5 cut(s)	11, 14, 69, 511, 1415
LweI	GCATC	6 cut(s)	82, 349, 1369, 1507, 1658, 1903
MaeI	CTAG	4 cut(s)	609, 911, 1035, 1557
MaeII	ACGT	1 cut(s)	1330
MaeIII	GTNAC	6 cut(s)	347, 445, 1366, 1705, 1742, 1835
MboII	GAAGA	8 cut(s)	136, 445, 845, 1221, 1388, 1623, 1688, 2226
MfeI	CAATTG	3 cut(s)	623, 819, 2175
MflI	RGATCY	2 cut(s)	733, 1820
MhlI	GDGCHC	3 cut(s)	1095, 1327, 2049
MlyI	GAGTC	3 cut(s)	278, 511, 2122
MmeI	TCCRAC	7 cut(s)	34, 149, 261, 567, 788, 1919, 2128
Mph1103I	ATGCAT	1 cut(s)	802
MroI	TCCGGA	2 cut(s)	107, 1195
MseI	TTAA	7 cut(s)	156, 791, 1164, 1530, 1608, 1701, 2186
MslI	CAYNNNNRTG	1 cut(s)	852
MspA1I	CMGCKG	2 cut(s)	1253, 1543
MspCI	CTTAAG	1 cut(s)	1163
MspI	CCGG	5 cut(s)	108, 295, 1196, 1371, 1419
MspR9I	CCNGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
MunI	CAATTG	3 cut(s)	623, 819, 2175
Mva1269I	GAATGC	1 cut(s)	1495
MvaI	CCWGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
MwoI	GCNNNNNNNGC	3 cut(s)	56, 1409, 1472
NcoI	CCATGG	1 cut(s)	827
NdeI	CATATG	1 cut(s)	1898
NlaIV	GGNNCC	7 cut(s)	222, 1338, 1339, 1511, 1822, 2007, 2193
NmuCI	GTSAC	4 cut(s)	347, 1366, 1742, 1835
NsiI	ATGCAT	1 cut(s)	802
NspI	RCATGY	2 cut(s)	727, 1297
NspV	TTCGAA	1 cut(s)	331
OliI	CACNNNNGTG	1 cut(s)	852
PasI	CCCWGGG	1 cut(s)	401
PciI	ACATGT	2 cut(s)	723, 1293
PciSI	GCTCTTC	1 cut(s)	1234
PcsI	WCGNNNNNNNCGW	3 cut(s)	296, 1318, 1327
PctI	GAATGC	1 cut(s)	1495
PfeI	GAWTC	5 cut(s)	212, 957, 1049, 1586, 2219
PflFI	GACNNNGTC	1 cut(s)	716
PinAI	ACCGGT	1 cut(s)	294
PkrI	GCNGC	6 cut(s)	26, 29, 84, 526, 1075, 1405
PleI	GAGTC	3 cut(s)	277, 511, 2121
PpsI	GAGTC	3 cut(s)	277, 511, 2121
Ppu21I	YACGTR	1 cut(s)	1331
PpuMI	RGGWCCY	4 cut(s)	221, 712, 919, 1337
PscI	ACATGT	2 cut(s)	723, 1293
PsiI	TTATAA	1 cut(s)	396
Psp5II	RGGWCCY	4 cut(s)	221, 712, 919, 1337
Psp6I	CCWGG	6 cut(s)	248, 400, 760, 841, 1857, 1946
PspFI	CCCAGC	1 cut(s)	1736
PspGI	CCWGG	6 cut(s)	248, 400, 760, 841, 1857, 1946
PspN4I	GGNNCC	7 cut(s)	222, 1338, 1339, 1511, 1822, 2007, 2193
PspPPI	RGGWCCY	4 cut(s)	221, 712, 919, 1337
PsuI	RGATCY	2 cut(s)	733, 1820
PsyI	GACNNNGTC	1 cut(s)	716
PvuII	CAGCTG	2 cut(s)	1253, 1543
RsaI	GTAC	3 cut(s)	869, 928, 1005
RsaNI	GTAC	3 cut(s)	868, 927, 1004
RseI	CAYNNNNRTG	1 cut(s)	852
Rsr2I	CGGWCCG	1 cut(s)	292
RsrII	CGGWCCG	1 cut(s)	292
SalI	GTCGAC	1 cut(s)	719
SapI	GCTCTTC	1 cut(s)	1234
SaqAI	TTAA	7 cut(s)	156, 791, 1164, 1530, 1608, 1701, 2186
SatI	GCNGC	6 cut(s)	25, 28, 83, 525, 1074, 1404
SchI	GAGTC	3 cut(s)	278, 511, 2122
ScrFI	CCNGG	6 cut(s)	250, 402, 762, 843, 1859, 1948
SduI	GDGCHC	3 cut(s)	1095, 1327, 2049
SfaNI	GCATC	6 cut(s)	82, 349, 1369, 1507, 1658, 1903
SfcI	CTRYAG	1 cut(s)	1171
SfuI	TTCGAA	1 cut(s)	331
SinI	GGWCC	6 cut(s)	221, 292, 712, 824, 919, 1337
SmiMI	CAYNNNNRTG	1 cut(s)	852
SmlI	CTYRAG	3 cut(s)	1102, 1163, 1759
SmoI	CTYRAG	3 cut(s)	1102, 1163, 1759
SsiI	CCGC	2 cut(s)	1074, 1504
SspMI	CTAG	4 cut(s)	609, 911, 1035, 1557
StyD4I	CCNGG	6 cut(s)	248, 400, 760, 841, 1857, 1946
StyI	CCWWGG	3 cut(s)	827, 922, 1341
TaaI	ACNGT	5 cut(s)	999, 1175, 1448, 1711, 2044
TaiI	ACGT	1 cut(s)	1333
TaqI	TCGA	9 cut(s)	147, 287, 309, 331, 423, 720, 1301, 1730, 1904
TatI	WGTACW	2 cut(s)	867, 1003
TauI	GCSGC	1 cut(s)	1076
TfiI	GAWTC	5 cut(s)	212, 957, 1049, 1586, 2219
Tru1I	TTAA	7 cut(s)	156, 791, 1164, 1530, 1608, 1701, 2186
Tru9I	TTAA	7 cut(s)	156, 791, 1164, 1530, 1608, 1701, 2186
TscAI	CASTG	2 cut(s)	1180, 1453
TseFI	GTSAC	4 cut(s)	347, 1366, 1742, 1835
TseI	GCWGC	5 cut(s)	24, 27, 82, 524, 1403
Tsp45I	GTSAC	4 cut(s)	347, 1366, 1742, 1835
TspDTI	ATGAA	4 cut(s)	17, 431, 453, 1259
TspGWI	ACGGA	3 cut(s)	305, 1058, 1979
TspRI	CASTG	2 cut(s)	1180, 1453
Tth111I	GACNNNGTC	1 cut(s)	716
Vha464I	CTTAAG	1 cut(s)	1163
VneI	GTGCAC	1 cut(s)	1323
VpaK11BI	GGWCC	6 cut(s)	221, 292, 712, 824, 919, 1337
XapI	RAATTY	4 cut(s)	138, 572, 1627, 2011
XceI	RCATGY	2 cut(s)	727, 1297
XcmI	CCANNNNNNNNNTGG	1 cut(s)	167
XmiI	GTMKAC	2 cut(s)	720, 2116
XspI	CTAG	4 cut(s)	609, 911, 1035, 1557
Zsp2I	ATGCAT	1 cut(s)	802

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.